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26 results about "Monooxygenase" patented technology
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Monooxygenases are enzymes that incorporate one hydroxyl group into substrates in many metabolic pathways. In this reaction, the two atoms of dioxygen are reduced to one hydroxyl group and one H₂O molecule by the concomitant oxidation of NAD(P)H. One important subset of the monooxygenases, the cytochrome P450 omega hydroxylases, is used by cells to metabolize arachidonic acid (i.e. eicosatetraenoic acid) to the cell signaling molecules, 20-hydroxyeicosatetraenoic acid or to reduce or totally inactivate the activate signaling molecules for example by hydroxylating leukotriene B4 to 20-hydroxy-leukotriene B5, 5-hydroxyeicosatetraenoic acid to 5,20-dihydroxyeicosatetraenoic acid, 5-oxo-eicosatetraenoic acid to 5-oxo-20-hydroxyeicosatetraenoic acid, 12-hydroxyeicosatetraenoic acid to 12,20-dihydroxyeicosatetraenoic acid, and epoxyeicosatrienoic acids to 20-hydroxy-epoxyeicosatrienoic acids.
Compounds and methods for persistently increasing PAM
This invention relates to increasing the level of peptidyl glycine α-amidyl monooxygenase (PAM) in human circulation as a long-acting drug and for treating subjects, wherein said treatment includes: reducing the likelihood or risk of disease or condition, and / or reducing the occurrence of disease or condition, and / or reducing the severity of disease or condition.
Owner:PAM THERAPEUTICS & DIAGNOSTICS LTD
A method of facilitating transport to increase retinyl synthesis
The application discloses a method for promoting transport and improving retinal synthesis, which is obtained by introducing an exogenous ABC transport protein Pdr5 into a host fungus and adding a copy of beta-carotene-15, 15'-monooxygenase BLH; wherein the host fungus is a genetically modified Pichia pastoris strain PP-BCCIV. The application further verifies the retinal production performance of the recombinant strain by screening different surfactants, and further improves the ability of Pichia pastoris to produce retinal. At the same time, all the retinal is extracted into the extracellular, and all the carotenes are left in the intracellular, so as to achieve the co-production of carotenes and retinal. The recombinant Pichia pastoris of the application has a simple construction method, can better promote the synthesis of retinal and other vitamin A, and can make the engineering strain utilize glucose and methanol to efficiently synthesize retinal through a 5 L fermentation tank, which is beneficial to industrial production.
Owner:NANJING TECH UNIV
Molecular marker of wheat kernel type related gene ta cyt-5a and application
ActiveCN118879908BMicrobiological testing/measurementPlant genotype modificationStart codonCoding region
The application relates to the technical field of wheat kernel type genes, and provides a wheat kernel type gene TaCYT-5A TaCYT-5A The application of the molecular marker is KASP-TaCYT-5A, the marker is a SNP site (C / T) detected at 495818646 bp in a QTL cluster for controlling the wheat kernel type and kernel length traits of a 5A chromosome 484432128-495883712 bp, that is, a 4-hydroxyphenylacetaldehyde oxime monooxygenase gene TaCYT-5A The coding region is 124 bp away from the start codon. The functional KASP molecular marker of the wheat 4-hydroxyphenylacetaldehyde oxime monooxygenase gene TaCYT-5A provided by the application can be used for identifying whether an TaCYT-5A excellent allele exists in a wheat variety / strain, and applied in assisted selection breeding and breeding in combination with other known kernel type related genes.
Owner:GANSU AGRI UNIV
2,3-dihydro-4h-benzo[b][1,4]oxazin-4-yl)(5-(phenyl)-pyridin-3-yl)methanone derivatives and analogous compounds as cyp11a1 inhibitors for the treatment of prostate cancer
The present invention relates to compounds of formula (I) wherein A is a 3-10 membered carbocyclic ring or a 4-12 membered heterocyclic ring containing 1-4 heteroatoms selected from O, N or S; B is any one of the following groups (1), (2) or (3); C is any one of the following groups (1'), (2'), (3') or (4'); G1 is CH2, NH or O; G2 and G3 are independently of each other CH or N; Z is -C(O)-, -SO2-, -C 1‑3 alkyl- or -CH2-C(O)-; L is a bond, -C 1‑7 alkyl- or -C 1‑7 alkenyl-. The compounds of formula (I) are inhibitors of cytochrome P450 monooxygenase 11A1 (CYP11A1). The compounds are useful as medicaments for the treatment of steroid receptor, e.g. androgen receptor or estrogen receptor dependent diseases and disorders such as cancer including prostate cancer and estrogen cancer.
![2,3-dihydro-4h-benzo[b][1,4]oxazin-4-yl)(5-(phenyl)-pyridin-3-yl)methanone derivatives and analogous compounds as cyp11a1 inhibitors for the treatment of prostate cancer](https://images-eureka.patsnap.com/patent_img/11b7d4da-8984-4477-b1aa-15fd7bf9c345/QLYQS_1.png "2,3-dihydro-4h-benzo[b][1,4]oxazin-4-yl)(5-(phenyl)-pyridin-3-yl)methanone derivatives and analogous compounds as cyp11a1 inhibitors for the treatment of prostate cancer")
![2,3-dihydro-4h-benzo[b][1,4]oxazin-4-yl)(5-(phenyl)-pyridin-3-yl)methanone derivatives and analogous compounds as cyp11a1 inhibitors for the treatment of prostate cancer](https://images-eureka.patsnap.com/patent_img/11b7d4da-8984-4477-b1aa-15fd7bf9c345/QLYQS_2.png "2,3-dihydro-4h-benzo[b][1,4]oxazin-4-yl)(5-(phenyl)-pyridin-3-yl)methanone derivatives and analogous compounds as cyp11a1 inhibitors for the treatment of prostate cancer")
![2,3-dihydro-4h-benzo[b][1,4]oxazin-4-yl)(5-(phenyl)-pyridin-3-yl)methanone derivatives and analogous compounds as cyp11a1 inhibitors for the treatment of prostate cancer](https://images-eureka.patsnap.com/patent_img/11b7d4da-8984-4477-b1aa-15fd7bf9c345/QLYQS_3.png "2,3-dihydro-4h-benzo[b][1,4]oxazin-4-yl)(5-(phenyl)-pyridin-3-yl)methanone derivatives and analogous compounds as cyp11a1 inhibitors for the treatment of prostate cancer")
Owner:ORION CORP(FI)
A bacteria-enzyme complex microbial agent, a preparation method and application thereof
本发明涉及微生物菌肥技术领域，特别是涉及一种菌酶复合微生物菌剂及其制备方法和应用。本发明针对田间秸秆还田腐解慢、影响下茬作物生长的问题，提供了一种菌酶复合微生物菌剂。所述菌酶复合微生物菌剂由草酸青霉（Penicillium oxalicum）菌株F12的孢子悬液与裂解性多糖单加氧酶（LPMO）按特定配比复配而成。田间施用时，将秸秆粉碎抛撒后，通过无人机喷洒菌剂，翻压入土，并调节土壤相对湿度。本发明解决了秸秆腐解慢的技术难题，有助于促进种子出苗和减少病虫害发生，可在秸秆还田场景和菌剂生产企业中推广应用。
Owner:ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES +2
A gonicoccus strain yf2 degrading polyethylene mulch, microbial preparation, method and application
This invention relates to the field of microbial technology, disclosing a *Strombus adhesiveus* strain YF2 that degrades polyethylene mulch film, microbial preparations, methods, and applications, addressing the problems of insufficient efficacy and unclear mechanisms in existing PE-degrading strains. This strain is deposited at the Guangdong Provincial Microbial Culture Collection Center, with accession number GDMCC No:67086, and a deposit date of October 13, 2025. Through systematic parallel comparisons, strain YF2 significantly outperformed the control strain in PE degradation efficiency, biofilm formation ability, and induction of PE polymer oxidation. Genomic analysis showed that YF2 contains a genetic network encoding key degrading enzyme systems such as nitroreductase, cytochrome P450, and alkane monooxygenase, and particularly possesses unique nitrification degradation potential. Microbial preparations prepared based on this strain can be used for the bioremediation of PE mulch film residues in farmland soils, compost, and other environments.
Owner:SHIHEZI UNIVERSITY
Double-stranded RNA and its use in the control of citrus aphids
This invention relates to the field of pest control technology, and discloses a double-stranded RNA and its application in the control of citrus aphids. The double-stranded RNA comprises a double-stranded RNA consisting of the nucleotide sequence shown in SEQ ID NO:2 and its reverse complementary nucleotide sequence; the nucleic acid molecule encoding the double-stranded RNA is shown in SEQ ID NO:1 or SEQ ID NO:3. The double-stranded RNA provided by this invention can simultaneously target the chitin synthase gene, cytochrome P450 monooxygenase gene, and epidermal protein gene of the brown citrus aphid, and simultaneously silence the expression of these three key genes through an RNA interference mechanism, leading to inhibited growth and development or death of the brown citrus aphid. Therefore, the double-stranded RNA provided by this invention can efficiently and specifically control the brown citrus aphid; it can reduce the use of chemical pesticides, is green and environmentally friendly, and safe and harmless.
Owner:SHENZHEN UNIV
Carbohydrolytic and lipogenic recombinant yeasts
Recombinant yeasts bioengineered to overexpress genes for utilization of cellulosic and hemicellulosic fibers and / or produce lipid, and methods of use thereof. The yeasts are modified to express, constitutively express, or overexpress an exo-cellobiohydrolase I, an exo-cellobiohydrolase II, an endoglucanase, an endoxylanase, or any combination thereof. The yeasts may also be modified to express, constitutively express, or overexpress a lytic polysaccharide monooxygenase, an arabionofuranosidase, a xylanases, an acetylxylan esterase, or any combination thereof. The methods include cultivating the recombinant yeasts to convert low value organic stillage byproducts such as cellulose and hemicellulose into lipids suitable for biodiesel production and other higher value uses.
Owner:XYLOME CORP
Kynurenine-3-monooxygenase inhibitors, pharmaceutical comositions, and methods of use thereof
Owner:CHDI FOUNDATION INC
Preparation method and application of chitooligosaccharide uronic acid
The application discloses a preparation method and application of chitooligosaccharide alduronic acid and belongs to the technical field of marine bioengineering. The preparation method comprises the following steps: after beta-chitin and a cleaving polysaccharide monooxygenase SmLPMO10 are dissolved in a Tris-hydrochloric acid buffer solution, a reducing agent is added, and an enzymatic reaction is carried out; after the reaction is carried out for 24 hours, the reducing agent is supplemented, the reaction is continuously carried out, the reducing agent is supplemented once every 24 hours, after the reaction is carried out for 96 hours, supernatant is obtained by filtration, and the chitooligosaccharide alduronic acid is obtained after inactivation, centrifugal removal of supernatant, desalination and freeze-drying. The chitin can be cleaved by using a single cleaving polysaccharide monooxygenase, without additional addition of chitinase, and the reaction condition is mild, so that the generation of by-products and environmental pollution are avoided. The application provides an environment-friendly preparation method of chitooligosaccharide alduronic acid, and the research and development of a green conversion technology of chitin and chitin-like biomass resources are greatly promoted.
Owner:INST OF OCEANOLOGY - CHINESE ACAD OF SCI
Method for rapidly identifying ganoderma lucidum stem length by gene expression amount
PendingCN122104979AMicrobiological testing/measurementMicroorganism based processesBiotechnologyMycelium
The application discloses a method for rapidly identifying Ganoderma lucidum stem length by using gene expression, and belongs to the technical field of Ganoderma lucidum breeding and cultivation. The application detects the relative expression of cytochrome P450 monooxygenase gene (P450 gene) and protein kinase gene (Pkinase gene) in Ganoderma lucidum mycelium, uses specific primers q0054762 and q0088881 to perform qPCR amplification, and establishes a correlation model between gene expression and stem phenotype, so that the length of Ganoderma lucidum stem can be rapidly and accurately predicted in the mycelium stage, traditional mushroom cultivation is not needed, the breeding cycle is significantly shortened, the detection cost is reduced, and the workload is reduced, and the application provides an efficient and simple technical means for molecular assisted breeding of Ganoderma lucidum.
Owner:INST OF BIOLOGICAL & MEDICAL ENG GUANGDONG ACAD OF SCI
Bayer Williger monooxygenase mutant and application thereof
The invention discloses a Bayer Williger monooxygenase mutant and an application of the Bayer Williger monooxygenase mutant. The invention provides a monooxygenase mutant, and compared with an amino acid sequence shown as SEQ ID NO: 1, an amino acid sequence of the monooxygenase mutant contains amino acid residue difference of one or more sites; the activity of the monooxygenase mutant is improved compared with that of Bayer-Williger monooxygenase with the amino acid sequence shown as SEQ ID NO: 1, wherein the amino acid sequence of the Bayer-Williger monooxygenase is shown as SEQ ID NO: 1. According to the monooxygenase mutant provided by the invention, the chiral thiosulfinate compound can be generated with relatively high yield and stereoselectivity, and the reaction condition is mild.
Owner:SHANGHAI SYNTHEALL PHARM CO LTD
Hydiitzia sp. c2 and application thereof
This invention relates to a strain of Hedizella ( Dietzia maris The strain C2, with its strain preservation number CCTCC NO: M2025275, is also described in this invention. This invention relates to the application of the above-mentioned strain in the biological treatment of organic pollution from petroleum and petrochemical industries. This strain, isolated from core samples from the Daqing Oilfield, can simultaneously secrete denitrification reductase and monooxygenase under aerobic conditions, allowing for simultaneous oxidation and reduction. AlkB hydroxylase is responsible for the degradation of long-chain alkanes (C5-C16), P450 monooxygenase is responsible for the oxidative degradation of benzene compounds and low-cyclic aromatic hydrocarbons, and denitrification reductase is responsible for the dechlorination of low-chlorinated organic compounds. It can significantly improve the degradation rate of recalcitrant components in petroleum, laying the foundation for the treatment of oily sludge, petroleum-contaminated soil, petroleum wastewater, and petrochemical wastewater, and providing a new avenue for the green and low-carbon remediation of petroleum and petrochemical wastewater and petroleum-contaminated soil in my country.
Owner:HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
Biosynthesis of hue-tunable indigo dyes
A biosynthetic method and composition for producing indigo dyes comprising indigotin and isatin. A tryptophanase (TRP) and a flavin monooxygenase (FMO) can be produced through a transgenic organism having carrier encoded tryptophanase and flavin monooxygenase. The produced tryptophanase and flavin monooxygenase convert L-tryptophan into indigo dyes in the presence of one or more bioactive additives selected from cysteine, 2-hydroxyindole, or 2-indoxyl, and the weight ratio of indigotin to isatin in the dyes is between 1:0.1 to 1:4. The dyes have a hue of X: 0.1920 to 0.2481, Y: 0.2537 to 0.2019, Z: 0.5543 to 0.5501 in the CIE XYZ color space definition. Selectable and repeatable dyeing hues are provided.
Owner:NANO & ADVANCED MATERIALS INST
Lytic polysaccharide monooxygenases and their use in detergents
The present invention relates to newly identified lytic polysaccharide monooxygenases (LPMOs) and their use as enzyme benefit enhancers in detergents, as well as detergent compositions comprising a combination of said LPMOs with a detersive enzyme.
Owner:NOVOZYMES AS
Lytic polysaccharide monooxygenase slpmo10 and use thereof
The application discloses a kind of lytic polysaccharide monooxygenase SLPMO10 and application thereof, belong to functional enzyme technical field.SLPMO10 amino acid sequence is as shown in SEQ ID NO.1, the nucleotide sequence of its encoding gene is as shown in SEQ ID NO.2.The enzyme belongs to AA10 family, optimum reaction temperature is 60 DEG C, optimum pH is 8.0, by C1 oxidation mode cleavage cellulose, and specifically produce polymeric degree 6 and 8 chitooligosaccharide acid.SLPMO10 and endoglucanase or complex cellulase synergistic effect, can significantly improve the degradation efficiency of cellulose;Especially with complex cellulase synergistic treatment seaweed powder, reducing sugar yield increases 23.5%.The application has important industrial application value in enzyme method treatment cellulose and seaweed biomass conversion.
Owner:OCEAN UNIV OF CHINA
Flavoenzyme-dependent monooxygenase mutants and their use in the production of indigo
This invention relates to a flavin-dependent monooxygenase mutant and its application in indigo production, belonging to the field of enzyme engineering technology. Based on a flavin-dependent monooxygenase derived from *Methvlophaga aminisulfidivorans*, this invention employs deep learning methods to modify protein molecules, obtaining multiple single- or multi-site mutants. Experimental verification of their catalytic effect on indigo synthesis yielded mutants with mutations at five specific amino acid sites: W319A, C78D, K198A, D197E, and Y320A. These mutants exhibit significantly enhanced catalytic activity compared to the wild type. Specifically, the three-site combination mutant K198A / D197E / Y320A showed a 2.58-fold increase in enzyme activity compared to the wild type. In indigo production, these mutants can effectively increase indigo yield.
Owner:JIANGNAN UNIV
Use of a small molecule compound KA-C1 in the preparation of a medicament
PendingCN122097353AOrganic active ingredientsCardiovascular disorderCardiac fibrosisProtein activity
The application belongs to the field of medicine, and specifically discloses application of a small molecule compound KA-C1 in preparation of a medicine for preventing or treating cardiac hypertrophy, cardiac fibrosis and / or heart failure. The application finds that kynurenine-3-monooxygenase (KMO) is an effective medicine target for treating pathological cardiac hypertrophy and cardiac fibrosis through research. Through computer-aided virtual screening, the application finds a KMO-specific agonist KA-C1, and KA-C1, as a medicine active ingredient, can directly stimulate KMO protein activity, thereby effectively resisting pathological cardiac hypertrophy and cardiac fibrosis, and further preventing or treating heart failure. Therefore, the discovery of the small molecule compound KA-C1 provides a new medicine target strategy for treating pathological cardiac hypertrophy, cardiac fibrosis and heart failure, and has important and wide application prospects.
Owner:ZHONGSHAN HOSPITAL FUDAN UNIV
Kynurenine-3-monooxygenase inhibitors, pharmaceutical composition, and methods of use thereof
Owner:CHDI FOUNDATION INC
Aspergillus niger polysaccharide lytic monooxygenase and use thereof in promoting rumen degradation of straw
ActiveCN121320276Bchange surface structurepromote growthBacteriaMicroorganism based processesRumenFodder
The application belongs to the technical field of animal feed processing, and particularly relates to an Aspergillus niger polysaccharide-lytic monooxygenase and application thereof in promoting rumen degradation of straw. The amino acid sequence of the Aspergillus niger polysaccharide-lytic monooxygenase is shown in SEQ ID NO. 2. The Aspergillus niger polysaccharide-lytic monooxygenase can change the surface structure of the straw, promote the growth of fiber degradation-related classification bacteria in the rumen, and improve the rumen degradation rate of the straw.
Owner:JIANGXI AGRICULTURAL UNIVERSITY
A method for preparing chitooligosaccharides based on combined degradation of two enzymes
Owner:SHANDONG UNIV
Certain kynurenine-3-monooxygenase inhibitors, pharmaceutical compositions, and methods of use thereof
Owner:CHDI FOUNDATION INC
Cyp11a1 inhibitors and uses thereof
PendingCN122234041AOrganic active ingredientsGroup 5/15 element organic compoundsPregnenoloneProstate cancer
This invention relates to compounds of Formula I and their isotopic forms, stereoisomers, tautomers, pharmaceutically acceptable salts, pharmaceutically acceptable solvates, hydrates, prodrugs, and polymorphs. The compounds of Formula I can be used as inhibitors of cytochrome P450 monooxygenase 11A1 (CYP11A1), effectively inhibiting the synthesis of testosterone and pregnenolone, and have good application prospects in the preparation of drugs for diseases such as prostate cancer and breast cancer.
Owner:ARROMAX PHARMATECH