4 results about "Transaldolase" patented technology

A method to make tertiary β-hydroxy α-amino acids. The method includes reacting a ketone substrate and a primary or secondary β-hydroxy α-amino acid with an L-threonine aldolase and / or an L-threonine transaldolase for a time, at a temperature, and at a pH wherein the reaction yields a tertiary β-hydroxy α-amino acid product.

The present invention relates to a recombinant microorganism for producing 1,3-propanediol and a method for producing 1,3-propanediol using same. More specifically, the present invention provides a recombinant microorganism having an improved ability to produce 1,3-propanediol through overexpression of a transaldolase-encoding gene (tal), a succinyl-CoA synthetase-encoding gene (sucCD), a cystathionine γ-synthase-encoding gene (metB), an aspartate ammonia lyase-encoding gene(aspA), an aspartate aminotransferase-encoding gene (aspB), or a functional fragment thereof, and a method for producing 1,3-propanediol using same.

The application discloses a Yarrowia lipolytica gene engineering bacterium for producing erythritol by glycerol, and the Yarrowia lipolytica gene engineering bacterium is obtained by random insertion mutation mediated by non-homologous end connection, and strains with high cell growth and high erythritol yield are screened. Further, the gene engineering bacterium overexpresses glycerol dehydrogenase gene GCY3 or ARA1 and dihydroxyacetone kinase gene DAK2 of a glycerol metabolism dihydroxyacetone pathway. Further, the gene engineering bacterium overexpresses transketolase gene TKL1 and transaldolase gene TAL1 of a non-oxidative pentose phosphate pathway. The application further discloses a construction method and application of the gene engineering bacterium. The superior gene engineering bacterium obtained by constructing a mutant library has the characteristics of tolerating high-concentration glycerol, and the substrate conversion rate and glycerol utilization rate are significantly higher than those of a starting strain.

The present invention relates to a recombinant cell. The recombinant cell may express a first enzyme, a second enzyme and a third enzyme and produce a nonstandard amino acid (nsAA). The first enzyme may be a L-threonine transaldolase (L-TTA) or a threonine aldolase (TAs). The second enzyme may be a phenylserine dehydratase or a threonine deaminase (TD). The third enzyme may be an aminotransferase (AT), a L-amino acid dehydrogenase (L-AADH) or a D-amino acid aminotransferase. At least one of the first enzyme, the second enzyme and the third enzyme may be heterologous to the recombinant cell. Also provided are a method for producing a nonstandard amino acid (nsAA) by the recombinant cell and a method for producing a nsAA in a container in the presence of the first enzyme, the second enzyme and the third enzyme.