The invention provides a method of making mangel immune polysaccharides and yeasts extract, belonging to the technique field of preparing biological products, such as polysaccharides, by yeasts. Said method includes the following steps: brewers' yeast residue bitter-removing, foreign substance-removing, brewers' yeast autolysis, brewers' yeast complex enzymolysis and deactivation, segregation of polysaccharides and protein hydrolysate liquid, blink spray drying of mangel immune polysaccharides and yeasts extract, etc; it is characterized on: high yield, good quality, low cost, and could achieve the aim of preparing mangel immune polysaccharides and yeasts extract at the same time from identical yeast material, enhance the availability ratio of yeasts, avoid pollution to the environment in traditional method as a result of simply preparing one certain product and discharging other assistant liquid.