15 results about "Paraffin embedded" patented technology

The invention discloses application of jellyfish and snow rabbits in inhibition of ERK phosphorylation level and MAPK pathway and an evaluation method of the jellyfish and snow rabbits, and relates to the technical field of biological medicines.The method comprises the following steps that 1, a treated jellyfish and snow rabbits breast cancer cell group and a control breast cancer cell group are established; and step 2, respectively dividing the two groups of samples in the step 1 into two parts, fixing one part in paraformaldehyde with the mass concentration of 10%, treating, embedding in paraffin for immunohistochemical analysis, and quickly freezing the other part for transcriptomics analysis and the like. According to the invention, UPLC-MS is adopted to determine the main components of SMM, the influence of SMM on breast cancer (BC) is evaluated through in-vivo experiments, in order to deeply clarify the mechanism, network pharmacology and transcriptomics analysis are integrated, finally, immunohistochemistry (IHC) is adopted to verify and explore the exact breast cancer resisting mechanism of SMM, and a scientific basis is provided for potential clinical application of SMM.

The present disclosure encompasses methods for molecularly subtyping formalin-fixed paraffin-embedded tumor samples. The disclosure works particularly well for old and degraded (archival) samples for which standard methods are unfeasible. Further, the methods disclosed allow for the correlation of patient outcome data with the molecular subtype of the tumor and provides a wealth of information which will guide treatment decisions and / or selection of therapeutic agents.

The disclosure provides methods for the use of gene expression measurements to classify or identify neuroendocrine cancer in samples obtained from a subject in a clinical setting, such as in cases of formalin fixed, paraffin embedded (FFPE) samples.

Aspects of the present disclosure are directed to methods, compositions, and kits for detection and analysis of DNA and RNA cytosine methylation and / or RNA pseudouridylation. Certain aspects include methods, compositions and kits useful in bisulfite sequencing of methylated and / or pseudouridinylated nucleic acids, including methylated and / or pseudouridinylated nucleic acids from low-input samples such as cell-free DNA (cfDNA) and cell-free RNA, long fragment polynucleotides, and / or cfDNA and DNA from formalin-fixed paraffin-embedded (FFPE) samples. Also disclosed herein are methods, compositions and kits useful in bisulfite sequencing of methylated and / or pseudouridinylated nucleic acids with low nucleic acid damage, higher true positive results, and / or lower false negative results.

The application relates to an experimental method for improving the accuracy of laboratory pollution monitoring and the quality control capability in molecular pathology detection. The method comprises the following steps: preparing a blank wax block without any tissue component while preparing a tissue paraffin-embedded sample to be detected; slicing the tissue paraffin-embedded sample and the blank wax block respectively; extracting nucleic acid of the tissue sample and nucleic acid of the blank wax block respectively; carrying out nucleic acid amplification detection by taking the nucleic acid of the tissue sample as a sample to be detected, taking the nucleic acid of the blank wax block as a negative whole-process control, and combining a positive control; judging the pollution and analyzing the quality control of the whole experimental process according to the detection result of the negative whole-process control of the blank wax block, so that the whole-process pollution risk of molecular pathology detection is continuously monitored. The application has the effects of improving the accuracy of laboratory pollution monitoring, the reliability of experimental results and the whole-process quality control capability of the laboratory.

The invention discloses application of a detection reagent (a reagent for simultaneously detecting MET, PCDHGA3 and FAM3C) in detection of ZM fusion gene (PTPRZ1-MET fusion gene) positive secondary glioblastoma (sGBM), belongs to the technical field of biological medicine, and aims to solve the problems that an existing ZM fusion detection method is complex in operation, poor in specificity, not suitable for conventional clinical detection and the like. Based on high-throughput RNA sequencing and machine learning algorithm (XGBoost) analysis, it is found for the first time that a combined expression profile of the three genes MET, PCDHGA3 and FAM3C can remarkably distinguish ZM fusion positive and negative samples, the diagnosis accuracy (AUC) of the combined expression profile reaches up to 99.8%, and the combined expression profile has high sensitivity and specificity. The detection product is suitable for paraffin embedding (FFPE) pathological tissue samples and frozen samples, detection can be completed in combination with mature platforms such as multiple immunohistochemistry or high-throughput RNA sequencing, and good sample compatibility and operation feasibility are achieved.

The application discloses a method for relieving obesity cardiomyopathy lipid toxicity by Chemerin and belongs to the technical field of pharmacological research. Mice are selected to establish an obesity mouse model, and are equally divided into a control group, a high-fat diet group, an AAV CMKLR1 virus group, and a high-fat + AAV CMKLR1 virus group. In succession, mouse heart ultrasound, TSE system detection, glucose tolerance test, and insulin tolerance test are carried out, and mouse blood glucose is measured at intervals. Then, tissue protein is extracted for protein immunoblotting, and tissue RNA is extracted for reverse transcription, and then qPCR is used to detect the expression of related genes. After paraffin embedding and slicing of mouse tissues, HE, WGA, immunofluorescence and other pathological staining are carried out. MTBE method is used for lipid extraction and detection of lipid content. ELISA method is used to measure serum Chemerin, and Acyl-RAC method is used to detect protein palmitoylation. Finally, the experimental results are analyzed to draw a conclusion. The present application proves by clinical data and mouse models that Chemerin compensatorily increases in the process of obesity, and Chemerin can relieve cardiac lipid toxicity caused by obesity and protect cardiac function.

The present disclosure discloses a library construction method for detecting endometrial cancer-related gene mutations based on high-throughput sequencing, and belongs to the field of biotechnology. The method can detect the mutation types of endometrial cancer-related genes MSH2, PMS2, MLH1, MSH6 EPCAM, TP53, POLE, and PTEN in surgically removed fresh pathological tissues, formaldehyde-fixed and paraffin-embedded pathological tissues, paraffin sections, and specimens of whole blood, plasma, serum, and pleural effusion, etc. It may be used for multiple target sequences in a single tube to quickly complete the library construction. The entire library construction process only takes 3 hours, and the manual operation only needs 30 minutes. Combined with high-throughput sequencing, the platform may effectively solve the current difficulty in the detection of somatic multi-gene all-exon mutations in clinical endometrial cancer samples based on small numbers of clinical samples, and the cost is low.

This invention discloses a method for extracting cell nuclei from formaldehyde-fixed and paraffin-embedded samples, comprising: (1) extracting cell nuclei: cutting PA / PFA-fixed or FFPE samples into pieces, adding cell nuclei extraction solution, incubating, collecting the supernatant, centrifuging, and discarding the supernatant; (2) washing cell nuclei: washing the cell nuclei precipitate from step 1) with PBS mix, centrifuging, discarding the supernatant, and resuspending the cell nuclei with PBS mix. The cell nuclei extracted by this method have advantages such as good morphological integrity, high purity, good RNA integrity, and high RNA yield, which are of great significance for transcriptomics studies of PA / PFA-fixed and FFPE samples.

Provided herein are methods for detecting the presence or absence of a chromosome rearrangement in a formalin-fixed paraffin embedded (FFPE) sample with no detectable genomic variant associated with cancer. In certain embodiments, the methods include selecting a sample and performing a nucleic acid analysis on the selected sample and detecting whether a chromosome rearrangement is present or absent in the selected sample according to the nucleic acid analysis. In preferred embodiments, the nucleic acid analysis is a method that preserves spatial-proximal contiguity information.

A method for processing tissue section images is provided. The method includes: (S1) providing a paraffin-embedded tissue block; (S2) determining at least three first coordinates on tissue sections to be sliced off from the paraffin-embedded tissue block, ablating at the first coordinates to form marker points on the tissue sections, and slicing the ablated tissue sections off from the paraffin-embedded tissue block; (S3) acquiring a slide image of each of the ablated tissue sections; and (S4) determining a second coordinate corresponding to each marker point on the slide image, comparing for each marker point the second coordinate with the first coordinate, and processing the slide image according to the comparison results. A system for performing the method for processing tissue section images is also provided.

The application relates to the field of organoid embedding technology, and particularly relates to a mold device for organoid embedding, which solves the problems of the inability to simplify the whole process of 'dehydration-embedding', the risk of organoid damage and position deviation in the prior art, and comprises a bottom plate and a top cover, the bottom plate and the top cover are detachably buckled and form an embedding groove; a plurality of dehydration channels are formed in the mold device to enable the embedding groove to communicate with the outside; the top cover is provided with a wax liquid flow guide mechanism, and the mold device further comprises a demolding mechanism, the demolding mechanism comprises a knob, a threaded rod and a demolding unit; the knob is rotationally connected to the bottom surface of the bottom plate, the top surface of the knob is fixedly connected with one end of the threaded rod, the demolding unit is sleeved on the threaded rod, and the threaded rod is rotated to lift or lower the demolding unit. The device has the dual functions of dehydration treatment and paraffin embedding, realizes in-situ operation of the whole process from dehydration to molding of the organoid sample, completely eliminates the manual transfer link, and avoids structural damage and position deviation of the pre-embedded sample.

The application discloses a kind of construction methods of sunlight dermatitis mouse model, specifically belongs to animal experiment model experimental technical field, the construction method includes the following steps: S01, ultraviolet lamp intensity measurement and selection;S02, the establishment of sunlight dermatitis model;S03, fresh tissue paraffin embedding section;S04, wax tissue section respectively by H-E staining experiment, Masson staining experiment, immunohistochemical staining experiment, transmission electron microscopy sheet observation, obtain the structure characteristic change of material organization.This construction method is by controlling the consistency of keeping radiation dose, to restore actual situation to a greater extent, provides real and reliable basis for developing sunlight dermatitis drug.

Provided herein is a multiplexed immunohistochemistry staining and digital pathology analysis dual-process quality control product, comprising a formalin-fixed paraffin-embedded (FFPE) tonsil sample, and a cell dispersion zone located in the center of the FFPE tonsil sample, the cell dispersion zone comprising dispersed PBMCs embedded with agarose gel and embedded with paraffin. Also provided herein are methods of preparing and using the quality control product.