16 results about "Fresh Tissue" patented technology

The invention discloses a preparation method of a beef frozen wet powder matrix standard substance, and solves the technical problems of poor authenticity, low fidelity, poor uniformity and high energy consumption when a beef ractopamine residual matrix standard substance is prepared in the prior art. Obtaining a ractopamine positive beef sample; performing acid discharge treatment on the beef sample; pretreating the beef subjected to acid discharge, and cutting the beef into diced meat with the particle size of 5-10mm; preparing a frozen wet powder matrix standard substance by adopting a multi-stage freezing and crushing and pulse screening process; and uniformly mixing the final product at a low temperature, sub-packaging, and storing in an environment of-40 DEG C or-80 DEG C in a sealing manner. The traditional freeze-dried powder process is abandoned, the frozen wet powder is used as a carrier form, and the original physicochemical characteristics of fresh tissues are perfectly reserved by the form.

The invention relates to the technical field of biology, in particular to a preserving fluid for preserving in-vitro fresh tissues and single cell activity and a preparation method thereof. The preserving fluid can preserve in-vitro fresh tissues and single cell activity at the low temperature of 4 DEG C, and comprises the following components: D-glucose, sodium chloride, potassium chloride, magnesium chloride, sodium dihydrogen phosphate, L-glutamine, L-histidine, L-phenylalanine, L-methionine, L-cysteine, L-glutamic acid, L-tryptophan, L-arginine, L-serine and L-alanine. The feed additive is prepared from L-propylamine-glutamine, vitamin E, vitamin C and bovine serum albumin. The tissue and cell viability preservation reagent has the following technical effects that the tissue and cell viability preservation reagent provided by the invention can stably preserve in-vitro fresh tissues under the condition of 4 DEG C, the viability of a single cell is at least 85% or above within 72 hours, and the tissue and cell viability preservation reagent is suitable for transportation and detection of third-party detection institutions and hospital samples; and a technical support is provided for a single cell sequencing experiment with a high motility rate requirement.

This invention discloses a primer pair for identifying *Ulmus pumila* and *Ulmus chinensis* germplasm and its application. The primer pair is Ulmus_p104 or / and Ulmus_p40. Fresh tissue samples of *Ulmus pumila* or *Ulmus chinensis* to be tested are collected, and DNA is extracted from the tissue samples. Using this DNA as a template, PCR amplification is performed using the primer pair. The amplification products are detected by electrophoresis. Based on the detection results, the sample is determined to be either *Ulmus pumila* or *Ulmus chinensis*. This invention utilizes SSR molecular markers from transcriptome sequencing to identify *Ulmus pumila* and *Ulmus chinensis* germplasm resources, which is beneficial for promoting the breeding of superior varieties of *Ulmus pumila* and *Ulmus chinensis* and accelerating the utilization of superior germplasm. The specific primers of this invention amplify tissues of *Ulmus chinensis* and *Ulmus pumila* respectively, and the difference in product length between the two is significant, indicating that these specific primers can effectively identify *Ulmus pumila* and *Ulmus chinensis* germplasm.

This application discloses a method for awakening dormant Sanghuang fruiting bodies and rapidly restoring their growth, comprising the following steps: Pretreatment: placing dormant Sanghuang fruiting bodies in an environment with a temperature of 18℃-20℃, and spraying water on the surface of the fruiting bodies to achieve a surface moisture content of 65%-75%; Cutting the surface tissue: using a sterile scalpel to cut the aged tissue layer on the surface of the pretreated Sanghuang fruiting bodies, exposing the fresh tissue wound. The cutting thickness is 0.5mm-1mm, and the cutting area accounts for 50%-90% of the surface area of ​​the non-growing area of ​​the Sanghuang fruiting body; Covering with film: covering the cut area of ​​the Sanghuang fruiting body with a dark film and allowing it to stand for 3-6 days; Conventional cultivation: removing the dark film and cultivating and managing the Sanghuang fruiting bodies under conventional cultivation conditions. This method shortens the awakening cycle of Sanghuang, improves the recovery growth rate and yield of Sanghuang fruiting bodies, and reduces the infection rate of miscellaneous bacteria.

The invention discloses a method for directly cryopreserving pig ear edge tissues by cycloastragenol and application of cycloastragenol, and relates to the technical field of refrigeration or cryopreservation. On the basis of an existing refrigerating fluid formula, the concentration of DMSO is reduced, high-glucose DMEM is used for replacing DPBS, meanwhile, trehalose, cycloastragenol and the like are added, and a brand-new refrigerating fluid formula is provided. The method has the following advantages: 1) an unfreezing solution and an equilibrium solution do not need to be additionally prepared; the unfreezing method is easy to operate, only simple melting is needed, and the method is suitable for large-scale preservation. And 3) the tissue does not need to be subjected to complex pretreatment, and only the tissue needs to be simply cleaned. The method has the advantages that the method is suitable for field sampling, strict sterile conditions are not needed, the cell survival rate, the cell apoptosis rate, the mitochondrial membrane potential, the active oxygen, the cell proliferation rate and the like are not obviously different from those of cells cultured from fresh tissue, and the volume of the supported cryopreserved tissue is the maximum cryopreserved volume in the cryopreserved pig tissue which can be found at present.

The invention provides a fresh tissue fixing device. The upper half part of the fresh tissue fixing device comprises a pressurizing device, a moisture absorption buffer layer, a first pressurizing layer and a first pressurizing adjusting piece; and the lower half part of the fresh tissue fixing device comprises a group of fixing boxes and a pressurizing infusion device. According to the fixing device for the fresh tissue, a slice specimen of the fresh tissue can be conveniently fixed, stored and taken out, the slice of the fresh tissue can be kept flat through pressurization, the state of the slice of the fresh tissue can be well kept through permeation of fixing liquid, and subsequent detection and observation are facilitated. The fixing device for the fresh tissue has very important application value.

Provided herein are methods for multiplexed in situ analysis of biomolecules in a tissue. In particular, provided herein are methods for multiplexed single-cell in situ protein and nucleic acid profiling in fixed or fresh tissues, and also allows the investigation of the different cell compositions and their spatial organizations in intact tissues through consecutive cycles of probe hybridization, fluorescence imaging, and signal removal.

The invention discloses a kit for extracting plant tissue cell nucleuses, and belongs to the technical field of biology. The kit comprises a cell nucleus lysis solution and a cell nucleus cleaning solution, the cell nucleus lysis solution is prepared from cane sugar, Tris-HCl with the pH value of 7-8, NaCl, MgCl2, a surfactant, DTT, RNase inhibitor and spermidine by using nuclease-free water, and the cell nucleus cleaning solution is prepared from sucrose, Tris-HCl with the pH value of 7-8, NaCl, MgCl2, a surfactant, DTT, RNase inhibitor and spermidine by using nuclease-free water. The cell nucleus cleaning solution is prepared from sucrose, Tris-HCl, NaCl, MgCl2, DTT (dithiothreitol), RNase inhibitor and spermidine by using nuclease-free water, wherein the pH (Potential of Hydrogen) of the Tris-HCl is 7-8. The kit is suitable for cell nucleus extraction of fresh tissues of taxus chinensis stems, the obtained cell nucleus is complete in nuclear membrane, stable in gene expression, high in purity and few in impurity, and the kit can be used for single cell sequencing.

The invention provides a lung cancer pleural invasion auxiliary diagnosis system and method based on a multiphoton microscopy technology, and belongs to the technical field of medical artificial intelligence and intraoperative pathology assistion.The method comprises the steps that multiphoton images of unstained paraffin sections, frozen sections and fresh tissue are collected through the multiphoton microscopy system; constructing a multi-source data set and extracting 142-dimensional tissue features; after cross-domain data enhancement of the generative adversarial network, semantic features extracted by the deep learning pre-training model are fused, and a pleura infringement state classifier is constructed; in the operation, a real-time image is obtained through a portable multi-photon system and input into a classifier to rapidly predict VPI negative and positive. According to the method, rapid, accurate and label-free diagnosis in the operation of the VPI is realized, real-time support is provided for decision-making of the resection range of the lung cancer operation, insufficient treatment or excessive resection is avoided, and the prognosis of a patient is effectively improved.

Provided herein are methods for multiplexed in situ analysis of biomolecules in a tissue. In particular, provided herein are methods for multiplexed single-cell in situ protein and nucleic acid profiling in fixed or fresh tissues, that allows the investigation of the different cell compositions and their spatial organizations in intact tissues through consecutive cycles of probe hybridization, fluorescence imaging, and signal removal.

The application provides a glass-like cryopreservation solution and application thereof, and belongs to the technical field of tissue cryopreservation. The glass-like cryopreservation solution provided by the application contains specific final concentrations of nitrogen ketone, cellobiose and alpha-lactose, and overcomes the problem of reduced activity of stem cells in the process of umbilical cord Wharton's jelly cryopreservation in the prior art. The glass-like cryopreservation solution provided by the application can make the cryopreserved tissue well preserved at low temperature, so that the tissue can produce a low degree of dehydration in a short time but does not affect physiological properties, and is more conducive to preservation. When the tissue contains stem cells, the glass-like cryopreservation solution provided by the application can effectively avoid the problem that the stem cells in the tissue are caused to die in large quantities due to ice crystal formation in the cryopreservation process, and when the tissue is resuscitated, the stem cells can be quickly extracted, without affecting the activity of the stem cells, and without significant difference compared with fresh tissue.

The application discloses a primer pair for identifying Ulmus pumila and Ulmus lamellosa germplasm and application thereof, wherein the primer pair is Ulmus_p20, Ulmus_p61 or / and Ulmus_p165. Fresh tissue samples of the Ulmus pumila or Ulmus lamellosa to be detected are collected, and DNA of the tissue samples is extracted. The DNA is used as a template, and PCR amplification is carried out by using the primer pair. The amplification product is detected by electrophoresis. According to the detection result, it is determined that the sample to be detected is Ulmus pumila or Ulmus lamellosa. The application uses SSR molecular markers of transcriptome sequencing to identify Ulmus pumila and Ulmus lamellosa germplasm resources, is beneficial to promoting Ulmus pumila and Ulmus lamellosa breeding, and accelerates the utilization process of excellent germplasm. The specific primers of the application are used for amplifying Ulmus lamellosa and Ulmus pumila tissues respectively, and the length difference of the products of the two is significant, which indicates that the specific primers can effectively identify Ulmus pumila and Ulmus lamellosa germplasm.

The invention discloses an efficient freezing slicing method suitable for large tissue and application of the efficient freezing slicing method in MALDI-MSI imaging, and the method comprises the following steps: pouring crushed dry ice into a first container, putting a second container into the first container, and filling a gap between the second container and the first container with the crushed dry ice; freezing liquid is poured into the second container, and the freezing liquid reaches a pre-cooled low-temperature state under the action of dry ice; pouring a pre-cooled CMC solution into the embedding container, putting the fresh tissue into the CMC solution, and enabling the CMC solution to submerge the fresh tissue; putting the embedding container into a freezing liquid for freezing treatment, so that the fresh tissues are preliminarily frozen and formed; then transferring into a refrigerator for storage; before slicing, the frozen and formed tissue is subjected to rewarming treatment and then is cut. According to the freezing method provided by the invention, the generation of ice crystals can be remarkably reduced, the slice quality is improved, and high-quality large-tissue frozen slices and accurate mass spectrum imaging are realized.

The utility model relates to the technical field of biology and new medicine, and discloses a storable, efficient and high-precision tissue cutter which comprises an upper cover, a bearing disc and a transverse cutting blade, wherein a cutting assembly is arranged at the lower end of the upper cover, an opening is formed in the upper end of the bearing disc, a transverse knife edge is formed in the side wall of the bearing disc, the bearing disc is used for bearing a tissue sample, the upper cover can cover the opening in the upper end of the bearing disc, and the cutting assembly is used for cutting the tissue sample on the bearing disc; the transverse cutting blade is used for stretching into the bearing disc through the transverse cutting edge so as to cut the tissue sample. According to the utility model, the problems that the existing tissue treatment process is complicated, the manual shearing precision is not enough, and time and labor are wasted are solved, the taken fresh tissue blocks can be directly put into the tool, a plurality of perfect organ precursors with high precision can be obtained by completing two-step operation, the initial activity of cells is retained, the waste and damage are reduced, and the working efficiency is improved. Time and labor are saved.

The application discloses a kind of construction methods of sunlight dermatitis mouse model, specifically belongs to animal experiment model experimental technical field, the construction method includes the following steps: S01, ultraviolet lamp intensity measurement and selection;S02, the establishment of sunlight dermatitis model;S03, fresh tissue paraffin embedding section;S04, wax tissue section respectively by H-E staining experiment, Masson staining experiment, immunohistochemical staining experiment, transmission electron microscopy sheet observation, obtain the structure characteristic change of material organization.This construction method is by controlling the consistency of keeping radiation dose, to restore actual situation to a greater extent, provides real and reliable basis for developing sunlight dermatitis drug.

Provided herein are methods for multiplexed in situ analysis of biomolecules in a tissue. In particular, provided herein are methods for multiplexed single-cell in situ protein and nucleic acid profiling in fixed or fresh tissues, and also allows the investigation of the different cell compositions and their spatial organizations in intact tissues through consecutive cycles of probe hybridization, fluorescence imaging, and signal removal.