123 results about "Fresh Tissue" patented technology

The invention discloses a probe set for detecting whole exons of extended genetic diseases. The probe set for detecting the whole exons of the extended genetic diseases comprises a standard whole exonprobe set, a whole genome copy number variation probe, and a mitochondrial loop full-length probe, and the genetic diseases comprise 6161 genetic diseases; the standard whole exon probe set can detect the genetic diseases caused by whole exon mutation, the genetic diseases comprise nervous system diseases, metabolic system diseases, endocrine system diseases, digestive system diseases, skeletal system diseases, urinary system diseases, immune system diseases, cardiovascular system diseases, blood system diseases, integument system diseases, ophthalmic system diseases, ear system diseases, respiratory system diseases, and genital system diseases; and the density of the mitochondrial probe is 6X; test samples comprise blood, fresh tissue, FFPE samples, and saliva. The invention discloses using method and kit and application of the probe set for detecting the whole exons of the extended genetic diseases.

Methods for decellularizing mammalian tissue for use in transplantation and tissue engineering. The invention includes methods for simultaneous application of an ionic detergent and a nonionic detergent for a long time period, which may exceed five days. One method utilizes SDS as the ionic detergent and Triton-X 100 as the nonionic detergent. A long rinse step follows, which may also exceed five days in length. This long duration, simultaneous extraction with two detergents produced tissue showing stress-strain curves and DSC data similar to that of fresh, unprocessed tissue. The processed tissue is largely devoid of cells, has the underlying structure essentially intact, and also shows a significantly improved inflammatory response relative to fresh tissue, even without glutaraldehyde fixation. Significantly reduced in situ calcification has also been demonstrated relative to glutaraldehyde fixed tissue. Applicants believe the ionic and non-ionic detergents may act synergistically to bind protein to the ionic detergent and may remove an ionic detergent-protein complex from the tissue using the non-ionic detergent. The present methods find one exemplary use in decellularizing porcine heart valve leaflet and wall tissue for use in transplantation.

A workstation that provides an efficient method to collect biological tissues in a column tissue array format from blocks of embedded, frozen tissues, or fresh tissues. The workstation has a control unit for directing operations of the workstation and the operation unit for performing the production of the tissue column array. The operation unit comprises an array of vertical tubes in a platform, an arbor which engages and presses down the designated tube in the array, the embedded tissue block which is mounted directly below the designated tube, assemblies of motors responsive to the control unit for driving the platform and the tissue block, a light source block for generating an alignment signal, and a light detector block which measures the signal from the light source to determine the degree of alignment between the arbor, punch tubes, and the specimen block.

An apparatus for sectioning fresh unfixed tissue into very thin layers with preserved tissue architecture, antigenicity, mRNA content, and amenable to 3-D computer reconstruction without mechanical or thermal damage by employing a sectioning tool having an electrode with an intense focused electrical field at an edge. A computer controlled x-y-z translation stage moves the sectioning tool through the tissue as defined by a predetermined program. The sectioning tool produces consecutive thin sections of fresh tissue for immunohistochemical and nucleic acids analyses without mechanical or thermal damage, ultimately allowing high-resolution volumetric reconstruction of gene and protein expression patterns of large tissue specimens. The geometry of the sectioning tool is selected so as to produce a spatially localized electrical field of sufficient intensity to sever molecular bonds or propagate flaws in tissue without mechanical cutting.

A rapid method of microwave-assisted formalin fixation of tissue that is described as: (a) a two-step process; (b) applicable to large (>5 mm thick) and small (<5 mm thick) tissues; (c) exposure of fresh tissue (i.e. a surgical biopsy) in a formalin solution to continuous microwave irradiation; (d) formalin circulated and cooled to maintain a constant temperature; (e) microwave-assisted formalin fixation performed at a temperature between 4° C. and 40° C.; (f) a temperature probe used to monitor fixative temperature in the cavity (g) after processing via microwave-assisted formalin fixation, the tissue can be processed by microwave methods or in an automatic tissue processor into paraffin for diagnostic evaluation. The two step process consists of a first low power microwave run followed by a second high power microwave run. An alternate embodiment of the present invention employing a one step MW oven process using other selected fixatives (glyoxal, glyoxal based and other aldehyde fixatives, alcohol, acetone, etc.)

The disclosure relates to the automatic staining, imaging, cutting and collection of samples in a sample-processing device, in particular in a microtome. It provides a general-purpose sample holder, which comprises fluid exchange means for, e.g., surface staining and washing of a specimen. Furthermore, it provides a microtome chamber comprising sample collection means. A directional flow is used in the microtome chamber to transport a cut section of the specimen to a sample collection chamber. The present microtome chamber is especially useful to automatically collect delicate microtome samples, such as thin fresh tissue slices prepared by a vibratome. The microtome chamber may further comprise a sample holder receptacle and means to transport fluids to and from the fluids exchange means of the sample holder.

The invention discloses a cigarette filter stick additive for reducing smoke polycyclic aromatic hydrocarbon and a preparation method and application thereof and belongs to the technical field of cigarette additives. Tobacco fresh tissue is used as raw materials, tobacco deoxyribonucleic acid (DNA) is extracted, the extracted DNA is dissolved in water, and the water solution is added in a section of a binary composite filter stick close to tobacco shred so as to enable the filter stick to hold back the polycyclic aromatic hydrocarbon in smoke. Through detection, content of the polycyclic aromatic hydrocarbon in the smoke of cigarettes adopting the cigarette filter stick additive can be reduced effectively, effect for reducing the smoke polycyclic aromatic hydrocarbon is improved along with increasing of addition amount of the cigarette filter stick additive, and the content of the polycyclic aromatic hydrocarbon in the smoke can be reduced by 25% when 2g DNA is added in each kilogram of filter sticks. Through sensory evaluation suction, cigarettes adopting the cigarette filter stick additive have no foreign smell during suction, and suction taste effect of the cigarettes is not influenced. The tobacco is used as extraction raw materials, and the cigarette filter stick additive is natural and safe, can be accepted by consumers easily, and has high application value.

Femtosecond laser pulses are used to iteratively cut and image fixed as well as exsanguinated fresh tissue. Such images help to automate three-dimensional histological analysis of biological tissue. Cuts are accomplished with approximately 0.3 to 100 microJoule pulses to ablate tissue with one-micrometer precision. Permeability, immunoreactivity, and optical clarity of the remaining tissue is retained after pulsed laser cutting. Samples from transgenic mice that express fluorescent proteins retained their fluorescence to within micrometers of the cut surface.

A workstation that provides an efficient method to collect biological tissues in a column tissue array format from blocks of embedded, frozen tissues, or fresh tissues. The workstation has a control unit for directing operations of the workstation and the operation unit for performing the production of the tissue column array. The operation unit comprises an array of vertical tubes in a platform, an arbor which engages and presses down the designated tube in the array, the embedded tissue block which is mounted directly below the designated tube, assemblies of motors responsive to the control unit for driving the platform and the tissue block, a light source block for generating an alignment signal, and a light detector block which measures the signal from the light source to determine the degree of alignment between the arbor, punch tubes, and the specimen block.

A mobile intra-operative microscopic diagnosis laboratory capable of analyzing fresh tissue specimens and providing intra-operative consultation within 20 minutes is described. The mobile laboratory is preferably a van and contains a cryostat for freezing the fresh tissue specimens and a means for cutting the specimens. In addition, it also contains the means for reading the slides to make microscopic diagnosis and a means for handling for fresh tissue and a means for indicating various locations in the specimen, preferably by inking them. It preferably contains a stainer for staining the samples and an intercom for communicating the microscopic diagnosis back to the operating room.

Systems and methods are provided for evaluating a fresh tissue sample, prepared as to fluoresce under illumination, during a medical procedure. A structured light source is configured to project a spatially patterned light beam onto the fresh tissue sample. An imaging system is configured to produce an image from fluorescence emitted from the illuminated fresh tissue sample. A system control is configured to provide a human-comprehensible clinically useful output associated with the medical procedure.

A mobile intra-operative microscopic diagnosis laboratory capable of analyzing fresh tissue specimens and providing intra-operative consultation within 20 minutes is described. The mobile laboratory is preferably a van and contains a cryostat for freezing the fresh tissue specimens and a means for cutting the specimens. In addition, it also contains the means for reading the slides to make microscopic diagnosis and a means for handling for fresh tissue and a means for indicating various locations in the specimen, preferably by inking them. It preferably contains a stainer for staining the samples and an intercom for communicating the microscopic diagnosis back to the operating room.

A tissue chip for detecting autoantibody is composed of substrate and the matrix on the substrate. Said matrix may be paraffin wax coated tissue, frozen fresh tissue, or cell specimen. An indirect immunofluorescence technique is used to detect, screen and analyze a great deal of autoantibodies in the blood serum of autoimmunopathy patient.

The invention provides a preserving fluid and a preserving method for maintaining high cell activity of a tissue sample, wherein the preserving fluid comprises the components: ion buffer solution components, carbohydrate components,, components avoiding the generation of ice crystals in and out solutions of tissue cells at a low temperature of 0-6 DEG C, components providing supplement, antioxidant and anti-apoptosis components for anabolism of the tissue cells; the preserving fluid has good biocompatibility, does not contain toxic or harmful components, and also does not contain components such as animal-derived protein, antibiotics and hormones influencing tissue gene expression. Different from traditional ultralow-temperature cryopreservation (-80 DEG C), the cryopreservation method disclosed by the invention can effectively realize low-temperature storage (0-6 DEG C) of fresh tissue samples of human beings and animals, maintains high cell activity of tissues, maintains tissue morphology, and is used for preservation and transportation of the tissue samples. Meanwhile, the nucleic acid integrity of the tissue and the stability of gene expression can be effectively maintained, the original state of the tissue is maintained, and the method can be used for gene detection, scientific research and other related experiments.

Fresh tissue slices can be cut from tissue specimens embedded in a low gel point agarose by the use of a vibrating, sliding-blade microtome. The microtome has a blade which vibrates in a generally horizontal direction and which is supported for movement in a generally diagonal, sliding direction with respect to the tissue specimen. The compound vibrating and sliding motion of the cutting blade of the microtome is very effective in cutting fresh tissue slices that have a slice viability, measured as a ratio of live to dead cells, which is much better than that which has been obtained using conventional vibrating microtomes.

The disclosed technology brings histopathology into the operating theatre, to enable real-time intra-operative digital pathology. The disclosed technology utilizes confocal imaging devices image, in the operating theatre, “optical slices” of fresh tissue—without the need to physically slice and otherwise process the resected tissue as required by frozen section analysis (FSA). The disclosed technology, in certain embodiments, includes a simple, operating-table-side digital histology scanner, with the capability of rapidly scanning all outer margins of a tissue sample (e.g., resection lump, removed tissue mass). Using point-scanning microscopy technology, the disclosed technology, in certain embodiments, precisely scans a thin “optical section” of the resected tissue, and sends the digital image to a pathologist rather than the real tissue, thereby providing the pathologist with the opportunity to analyze the tissue intra-operatively. Thus, the disclosed technology provides digital images with similar information content as FSA, but faster and without destroying the tissue sample itself.

Improved methods and systems for processing of solid tissue are described. The method may be performed manually or automatically. The system may have modules such as (i) a grossing module where a fresh tissue is sliced to prepare a tissue specimen, (ii) a hardening module that hardens the tissue specimen, (iii) an impregnating module that impregnates the tissue specimen that was hardened, and (iv) an embedding module that embeds a tissue specimen that was hardened and impregnated. Fresh (i.e., not fixed or frozen) tissue, which was excised to diagnose disease or to assess surgical treatment, is grossed to about 0.6 mm Preferably, the hardening of fresh tissue is initiated, but not completed, during grossing by contact with a chemical admixture. Preferably, dry ice, a thermoelectric device, or a gas condenser cools a metal mold containing the embedded specimen. It is sectioned and then microscopically examined as an alternative to histologic examination of a frozen section to avoid the known problems of discordant and deferred diagnosis.

The invention discloses a method for fully obtaining an interstitial ribosome nascent-chain complex. The method comprises the following steps: cleaning fresh tissues by using an isotonic saline liquid (containing translation extension inhibitors and magnesium ions) of 0-4 DEG C; removing an excessive liquid from the cleaned tissues, and then carrying out quick freezing treatment by using liquid nitrogen until the temperature of the tissues is stable; storing the quickly frozen tissues in an environment which is not higher than -80 DEG C; carrying out storage and transportation under the condition; and extracting RNC from the stored tissue samples, so as to obtain the interstitial ribosome nascent-chain complex. According to the method, interstitial RNC long-term storage and transportation can be carried out; the RNC of the tissue samples can be effectively obtained; and the restriction of time and space is broken through, thus convenience is provided for further research of the RNC and commercializing service of the RNC.

The invention relates to the field of the preparation of urethral stents, and discloses a polycaprolactone-collagen-fibroin nano three-dimensional porous stent and a preparation method thereof. The preparation method comprises the following steps of dissolving waste mulberry silk in a 0.1 percent of Na2CO3 aqueous solution, and repeatedly boiling for half an hour, so as to extract silk fibroin; dissolving the silk fibroin at a high temperature by using a ternary solution, dialyzing and filtering, and then putting at a room temperature for drying to form a regenerated silk fibroin membrane; dissolving the regenerated silk fibroin membrane, water-soluble collagen and polycaprolactone in hexafluoroisopropanol together, so as to form a mixed solution; carrying out electrostatic spinning on the mixed solution through a rotary receiving device, so as to obtain the nano three-dimensional porous stent, wherein a rotation speed is 300 to 500 revolutions per minute, and the diameter of a rotating shaft is 2 to 6 millimeters. The stent provided by the invention is convenient and efficient to process and manufacture, is convenient to apply, moreover, is convenient for adjusting various performances of a material, is stale in structure, and has certain mechanical strength to support the growth of a fresh tissue.

The invention aims to provide a solution for preserving and diluting DNA samples, and belongs to the technical field of polymerase chain reaction (PCR) and the category of dilution and preservation of RNA (DNA) samples. The solution is prepared by the following steps: adding certain amounts of DNA preservative and chemical reagents into deionized purified water; adjusting the pH value to be 7.8-8.2, and enabling the volume to be constant; sealing and conducting autoclaved sterilization for later use at the room temperature. The solution is white and transparent, has the advantages of being stable, easy to preserve, convenient to use and the like, and is suitable for preserving and diluting the DNA samples of serum, plasma, tissue, urine, secreta and the like. When immersed into the solution for diluting and preserving the DNA samples, DNA in fresh tissue cells can be preserved for 1 week at 37 DEG C, 1 month at 25 DEG C, 6 months at 4 DEG C and long term at minus 20 DEG C to minus 80 DEG C in good condition.

The invention relates to a method using umbilical cord tissue cryopreservation to preserve various stem cells, a thawing method and application. The method specifically includes: cleaning umbilical cord tissue, shearing into tissue sections, and stripping mesenchyme Wharton's jelly tissue; using an alpha-MEM protecting agent containing DMSO for preprocessing, performing concentration balance under4-10 DEG C for 10-20 minutes, performing low-temperature centrifuging, and removing supernate; adding dextran or fetal calf serum to allow the dextran or fetal calf serum and the alpha-MEM protectingagent containing DMSO to jointly form cryopreservation liquid, and using a program-controlled temperature lower or graded temperature lowering manner to perform cryopreservation on the tissue. The cryopreservation method has the advantages that the cryopreserved umbilical cord tissue can be effectively protected, subsequent thawing operation is facilitated, and the activity of cells cultured by the thawed tissue is the same as that of cells in fresh tissue.

The invention relates to a burn dressing applicable to department of burn and having an antibacterial effect, and belongs to the technical field of medical dressings. The burn dressing is mainly prepared by the following steps: preparing a mixed solution which consists of algin acid, vitamin E, sodium ascorbyl phosphate, sodium polyacrylate and glycerol; preparing extracting solutions of herba portulacae, radix astragali and honeysuckle flower; and conducting a cross-linking reaction by virtue of a water-soluble polymer, starch, modified microcrystalline cellulose, water, an antibacterial agent and a cross-linking agent, so that the dressing is prepared. According to the burn dressing disclosed by the invention, the radix astragali has functions of replenishing qi to strengthen superficial body resistance, inducing diuresis and draining toxins, expelling pus, healing sores and promoting generation of fresh tissue. Astragaloside, as a main active ingredient in the radix astragali, has a certain effect on promoting healing of traumatic wounds; the herba portulacae is effective on promoting wound recovery and enhancing the antibacterial effect; the algin acid is capable of promoting absorption of medicines and improving a recovery effect; and the burn dressing, in the dosage form of a hydrogel, has a good effect on the recovery of burns.

The invention relates to a biological medical hemostatic material, in particular to a medical micro-hole composite hemostatic material and a preparing method thereof. The medical micro-hole composite hemostatic material is formed by collagen, hyaluronic acid, puffball conidial powder and hemostatic Chinese herb extracts, wherein the collagen can quickly combine with hemoglobin in blood to form hemostatic coagulum, the hyaluronic acid can timely absorb water in the blood to form hydrogel to plug exudation, and the puffball conidial powder contains a lot of porous fiber which can quickly absorb water in the blood and mechanically plug crevasses of a blood vessel and exudation of blood capillaries. The three matters can form physical barriers to isolate a wound and achieve effects of preventing adhesion and stopping bleeding timely. The hemostatic Chinese herb extracts have effects of stopping bleeding, resisting inflammation, resisting bacteria, easing pain, cooling blood, eliminating stasis, subduing swelling, performing convergence, activating a coagulating system and promoting wound healing. The four matters organically combine with each other, are obvious in hemostatic effect, are suitable for hemostasis of all kinds of wound, fresh tissue wound surface exudation, small blood vessel hemorrhage and bleeding areas and can prevent adhesion.

The invention discloses stationary liquid for preserving pathological tissues. The stationary liquid is prepared from, by weight, formalin, Na<2>HPO<4>.12H<2>O, NH<2>PO<4>.2H<2>O, sodium chloride, acetic acid and ethyl alcohol; the mentioned constituents are mixed into a solution to serve as the stationary liquid for preserving a product, and a collected pathological specimen and the stationary liquid are both contained in a container, so that the pathological specimen can be preserved and fixed. By means of the stationary liquid, pathological tissue preservation and fixation can be achieved by one step, the preservation and fixation effect of the pathological tissues is remarkably improved, influence on active substances is reduced, the problem that the medical worker needs to compound stationary liquid temporarily is solved, convenience and rapidness are achieved, compounding is not required, and the stationary liquid can be used just after the container is opened; when the stationary liquid is used for preserving the pathological tissues, the specimen with the fresh tissues, in particular to specimen tissues rich in fibers, can be expanded and fix chromatin rapidly, biological enzyme is not closed, and fixation for section staining is facilitated; the neutral environment of body fluid is simulated, the pathological tissues which are preserved for a long time remain fresh, and the color is not changed. Preservation and fixation of the pathological tissues can be more stable, and it is helpful to improve the pathological section detection effect.

The invention discloses a method for extracting essential oil from fresh tissues of plants, which comprises the following steps of: 1, adding a certain proportion of distilled water into the fresh tissues of the plants and then carrying out breaking and homogenate; 2, placing the product in the step 1 in a container, and adding an extracting agent, i.e., 30 to 60 DEG C petroleum ether, wherein the volume ratio of the petroleum ether to homogenate tissues is 1: (3-5); 3, placing an amplitude transformer of an ultrasonic wave generator in a long container for ultrasonic extraction; 4, separating a mixed liquor obtained in the step 3 by using a centrifugal machine with the speed of 3000 to 6000 turn / m, and taking a supernatant liquid; 5, decompressing the extracting liquid in the step 4 in a constant temperature water bath so as to remove an organic solvent, and obtaining oil; and 6, dewaxing the oil to obtain the essential oil. According to the method disclosed by the invention, the collection and extraction of oil components in the fresh tissues of the plants can be realized in one step; and few natural aroma of the prepared essential oil product is lost.

The invention relates to a rootstock grafting method for rapid rooting and germinating of Pitaya, which belongs to the field of agriculture. The technical scheme adopted includes the following steps:selecting rootstocks; cutting the rootstocks to be not less than 30cm, and obliquely cutting the fleshy stems at the bases to expose 3cm vascular bundles; removing all the thorns on the rootstocks; drying the rootstocks in a cool place for 24 hours; soaking the vascular bundles in compound solution for 48 hours to promote rooting, cutting scion branches to 2.5cm-long and 0.6cm-wide scions, flat-cutting the tops of the rootstocks to expose fresh tissues, making the opposite sides of the scion thorn buds fit the vascular bundles of the rootstocks, using adhesive tapes to tighten and bind the scion thorn buds, and using fresh-keeping films to wrap the top grafting parts of the rootstocks for moisturizing; and inserting the grafted rootstock vascular bundles into wet sand, keeping the room temperature at 30 DEG C, and waiting for 30 days. The rooting of cutting seedlings is promoted and the number of roots is increased through the compound solution, the disease resistance of seedlings is improved by grafting, and the yield of Pitaya is improved.

The invention provides an organoid technology-based individualized lung cancer primary cell culture method. According to the method, high-throughput screening is carried out tumor cell models in vitrothrough organoid models established in tumor tissues of patients, so as to instruct individualized treatment of the patients. Specifically, excised lung cancer fresh tissues are utilized to carry outorganoid culture to establish individualized lung cancer primary cell models and carry out authentication, so that basis is provided for accurate treatment evaluation of lung cancer.

The invention belongs to the technical field of gene detection, and particularly relates to a low-frequency mutation detection method based on a snapback primer technology. The method comprises: designing a snapback primer, preparing a DNA template, preparing a detection system, setting a reaction procedure and determining a Ts value, judging a result, and other steps. According to the present invention, the detection method is the snapback ARMS and snapback primer HRM combined low-frequency mutation detection technology, such that the detection sensitivity of 1:10000 or even higher is achieved, clinical requirements of pollution resistance, high sensitivity, strong specificity, easy performing and result judgment on the mutation detection technology are met, and the method can be used for gene screening, genotyping (SNP, deletion mutation, insertion mutation) and other fields of various types of tissues including fresh tissue samples, whole blood, serum, tissue slices and other samples.