7 results about "Fresh Tissue" patented technology

This invention discloses a primer pair for identifying *Ulmus pumila* and *Ulmus chinensis* germplasm and its application. The primer pair is Ulmus_p104 or / and Ulmus_p40. Fresh tissue samples of *Ulmus pumila* or *Ulmus chinensis* to be tested are collected, and DNA is extracted from the tissue samples. Using this DNA as a template, PCR amplification is performed using the primer pair. The amplification products are detected by electrophoresis. Based on the detection results, the sample is determined to be either *Ulmus pumila* or *Ulmus chinensis*. This invention utilizes SSR molecular markers from transcriptome sequencing to identify *Ulmus pumila* and *Ulmus chinensis* germplasm resources, which is beneficial for promoting the breeding of superior varieties of *Ulmus pumila* and *Ulmus chinensis* and accelerating the utilization of superior germplasm. The specific primers of this invention amplify tissues of *Ulmus chinensis* and *Ulmus pumila* respectively, and the difference in product length between the two is significant, indicating that these specific primers can effectively identify *Ulmus pumila* and *Ulmus chinensis* germplasm.

This application discloses a method for awakening dormant Sanghuang fruiting bodies and rapidly restoring their growth, comprising the following steps: Pretreatment: placing dormant Sanghuang fruiting bodies in an environment with a temperature of 18℃-20℃, and spraying water on the surface of the fruiting bodies to achieve a surface moisture content of 65%-75%; Cutting the surface tissue: using a sterile scalpel to cut the aged tissue layer on the surface of the pretreated Sanghuang fruiting bodies, exposing the fresh tissue wound. The cutting thickness is 0.5mm-1mm, and the cutting area accounts for 50%-90% of the surface area of ​​the non-growing area of ​​the Sanghuang fruiting body; Covering with film: covering the cut area of ​​the Sanghuang fruiting body with a dark film and allowing it to stand for 3-6 days; Conventional cultivation: removing the dark film and cultivating and managing the Sanghuang fruiting bodies under conventional cultivation conditions. This method shortens the awakening cycle of Sanghuang, improves the recovery growth rate and yield of Sanghuang fruiting bodies, and reduces the infection rate of miscellaneous bacteria.

The invention discloses a method for directly cryopreserving pig ear edge tissues by cycloastragenol and application of cycloastragenol, and relates to the technical field of refrigeration or cryopreservation. On the basis of an existing refrigerating fluid formula, the concentration of DMSO is reduced, high-glucose DMEM is used for replacing DPBS, meanwhile, trehalose, cycloastragenol and the like are added, and a brand-new refrigerating fluid formula is provided. The method has the following advantages: 1) an unfreezing solution and an equilibrium solution do not need to be additionally prepared; the unfreezing method is easy to operate, only simple melting is needed, and the method is suitable for large-scale preservation. And 3) the tissue does not need to be subjected to complex pretreatment, and only the tissue needs to be simply cleaned. The method has the advantages that the method is suitable for field sampling, strict sterile conditions are not needed, the cell survival rate, the cell apoptosis rate, the mitochondrial membrane potential, the active oxygen, the cell proliferation rate and the like are not obviously different from those of cells cultured from fresh tissue, and the volume of the supported cryopreserved tissue is the maximum cryopreserved volume in the cryopreserved pig tissue which can be found at present.

The invention provides a fresh tissue fixing device. The upper half part of the fresh tissue fixing device comprises a pressurizing device, a moisture absorption buffer layer, a first pressurizing layer and a first pressurizing adjusting piece; and the lower half part of the fresh tissue fixing device comprises a group of fixing boxes and a pressurizing infusion device. According to the fixing device for the fresh tissue, a slice specimen of the fresh tissue can be conveniently fixed, stored and taken out, the slice of the fresh tissue can be kept flat through pressurization, the state of the slice of the fresh tissue can be well kept through permeation of fixing liquid, and subsequent detection and observation are facilitated. The fixing device for the fresh tissue has very important application value.

The invention provides a lung cancer pleural invasion auxiliary diagnosis system and method based on a multiphoton microscopy technology, and belongs to the technical field of medical artificial intelligence and intraoperative pathology assistion.The method comprises the steps that multiphoton images of unstained paraffin sections, frozen sections and fresh tissue are collected through the multiphoton microscopy system; constructing a multi-source data set and extracting 142-dimensional tissue features; after cross-domain data enhancement of the generative adversarial network, semantic features extracted by the deep learning pre-training model are fused, and a pleura infringement state classifier is constructed; in the operation, a real-time image is obtained through a portable multi-photon system and input into a classifier to rapidly predict VPI negative and positive. According to the method, rapid, accurate and label-free diagnosis in the operation of the VPI is realized, real-time support is provided for decision-making of the resection range of the lung cancer operation, insufficient treatment or excessive resection is avoided, and the prognosis of a patient is effectively improved.

Provided herein are methods for multiplexed in situ analysis of biomolecules in a tissue. In particular, provided herein are methods for multiplexed single-cell in situ protein and nucleic acid profiling in fixed or fresh tissues, that allows the investigation of the different cell compositions and their spatial organizations in intact tissues through consecutive cycles of probe hybridization, fluorescence imaging, and signal removal.

The application discloses a kind of construction methods of sunlight dermatitis mouse model, specifically belongs to animal experiment model experimental technical field, the construction method includes the following steps: S01, ultraviolet lamp intensity measurement and selection;S02, the establishment of sunlight dermatitis model;S03, fresh tissue paraffin embedding section;S04, wax tissue section respectively by H-E staining experiment, Masson staining experiment, immunohistochemical staining experiment, transmission electron microscopy sheet observation, obtain the structure characteristic change of material organization.This construction method is by controlling the consistency of keeping radiation dose, to restore actual situation to a greater extent, provides real and reliable basis for developing sunlight dermatitis drug.