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Embedding medium suitable for plant tissue frozen section and frozen section method

A technology of frozen section and plant tissue, applied in the field of analysis, can solve the problems of inability to use ethanol, inability to dissolve target chemical substances in alcohol substances, dehydration, etc., and achieve the effect of low price, clear microscopic results, and smooth slices

Inactive Publication Date: 2012-12-12
HUNAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This new technology allows researchers to study plants without damaging them during their preparation process by creating tiny sections from frostbaked samples or other materials like roots. These small areas are very easy to see because they have no visible signs on top when observed under microscope. They also maintain this shape even after being dried up due to water evaporation. Overall, these technical improvements make studying crops more accessible and cost-effective compared to previous methods such as cutting out parts off at different locations.

Problems solved by technology

This patented technical solution described by this patents relates to improving ice core technologies during various experiments involving studying plants' physiologically active components or proteins. However, current techniques have limitations making them less effective than desired because they require specialized equipment like freezing section tools. Therefore, new ways were needed to develop better solutions without requiring extensive laboratory resources.

Method used

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  • Embedding medium suitable for plant tissue frozen section and frozen section method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] (1) Preparation of embedding agent

[0015] The embedding agent is made of the following raw materials: polyvinyl alcohol 9g; Polyethylene glycol 2g; Sodium carboxymethyl cellulose 1.0g; Glycerol 2g; Formaldehyde 4g; Collagen 0.8g; Plant gel 0.4 g; deionized water 100g; when preparing, first add polyvinyl alcohol into deionized water, fully heat and dissolve, and when the temperature drops to 80°C, add the above-mentioned other raw materials in turn, heat and dissolve in a water bath, and then cool down to room temperature for later use.

[0016] (2) Frozen embedding and sectioning

[0017] Turn on the cooling switch of the frozen ultrathin microtome (YD-1900, produced by Zhejiang Jinhua Yidi Medical Equipment Factory), lower the temperature of the box and maintain it at -30°C, and when the temperature of the freezing table is lowered to -20°C, in order to observe the proanthocyanidins Distribution in rapeseeds, fresh mustard rapeseeds 25 days after pollination were hi...

Embodiment 2

[0023] (1) Preparation of embedding agent

[0024] The embedding agent is made of the following raw materials: polyvinyl alcohol 8g; polyethylene glycol 1g; sodium carboxymethyl cellulose 0.5g; glycerol 1g; formaldehyde 7g; collagen 0.5g; plant gel 0.9 g; 90g of deionized water; when preparing, first add polyvinyl alcohol into deionized water, fully heat and dissolve, and when the temperature drops to 90°C, add the above-mentioned other raw materials in turn, heat and dissolve in a water bath, and then cool down to room temperature for later use.

[0025] (2) Frozen embedding and sectioning

[0026] Turn on the cooling switch of the frozen ultrathin microtome (YD-1900, produced by Zhejiang Jinhua Yidi Medical Equipment Factory), lower the temperature of the box and maintain it at -20°C, and when the temperature of the freezing table is lowered to -40°C, in order to observe proanthocyanidins Distribution in rapeseeds, fresh mustard rapeseeds 25 days after pollination were hist...

Embodiment 3

[0031] (1) Preparation of embedding agent

[0032] The embedding agent is made of the following raw materials: polyvinyl alcohol 10g; polyethylene glycol 1.5g; sodium carboxymethyl cellulose 0.8g; glycerol 1.5g; formaldehyde 6g; collagen 0.6g; Glue 0.3g; deionized water 95g; when preparing, first add polyvinyl alcohol into deionized water, fully heat and dissolve, when the temperature drops to 85°C, add the other raw materials mentioned above one by one, heat and dissolve in a water bath, and then cool down to room temperature for later use.

[0033] (2) Frozen embedding and sectioning

[0034] Turn on the cooling switch of the frozen ultrathin microtome (YD-1900, produced by Zhejiang Jinhua Yidi Medical Equipment Factory), lower the temperature of the box and maintain it at -40°C, and when the temperature of the freezing table is lowered to -30°C, in order to observe the proanthocyanidins Distribution in rapeseeds, fresh mustard rapeseeds 25 days after pollination were histo...

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Abstract

Relating to methods able to conduct fast histochemistry and other analyses in plant tissues, the invention specifically relates to an embedding medium and a direct frozen section method. The embedding medium is composed of polyvinyl alcohol, polyethylene glycol, carboxymethylcellulose sodium, glycerol, formaldehyde, collagen, plant gel, and deionized water. According to the frozen section method, a plant material is not fixed, and a sample subjected to histochemical staining and other treatments or a fresh tissue is wrapped on a sample table directly to perform frozen section. The method solves the problem that in the microscopic observation of phytohistochemistry, immunolocalization, in-situ hybridization and other studies, a target chemical substance is easy to dissolve in a fixation agent, and is impossible to be fixed first and then sectioned, or the target chemical substance dissolves in an alcohol substance and is impossible to be dehydrated by ethanol.

Description

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Claims

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Application Information

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Owner HUNAN UNIV OF SCI & TECH
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