1303 results about "Microscopic observation" patented technology

A robot system for use in surgical procedures has two movable arms each carried on a wheeled base with each arm having a six of degrees of freedom of movement and an end effector which can be rolled about its axis and an actuator which can slide along the axis for operating different tools adapted to be supported by the effector. Each end effector including optical force sensors for detecting forces applied to the tool by engagement with the part of the patient. A microscope is located at a position for viewing the part of the patient. The position of the tool tip can be digitized relative to fiducial markers visible in an MRI experiment. The workstation and control system has a pair of hand-controllers simultaneously manipulated by an operator to control movement of a respective one or both of the arms. The image from the microscope is displayed on a monitor in 2D and stereoscopically on a microscope viewer. A second MRI display shows an image of the part of the patient the real-time location of the tool. The robot is MRI compatible and can be configured to operate within a closed magnet bore. The arms are driven about vertical and horizontal axes by piezoelectric motors.

The invention provides an implantable membrane for regulating the transport of analytes therethrough that includes a matrix including a first polymer; and a second polymer dispersed throughout the matrix, wherein the second polymer forms a network of microdomains which when hydrated are not observable using photomicroscopy at 400× magnification or less. In one aspect, the homogeneous membrane of the present invention has hydrophilic domains dispersed substantially throughout a hydrophobic matrix to provide an optimum balance between oxygen and glucose transport to an electrochemical glucose sensor.

There is disclosed an operation microscope in which an observing and displaying system of an operating instrument are selected, and an endoscope image for observing a dead angle of the microscope and a navigation image are selectively displayed in a microscope observation field, so that a tomographic image, three-dimensionally constructed image, and the like can be selectively displayed in a display screen in accordance with a treatment position displayed in a monitor or an observation position of the operation microscope.

There are provided modified and rounded graphite particles derived from scaly natural graphite particles by modification so as to bring their form close to a spherical form and satisfying all the following requirements (a) to (c): (a) that the degree of circularity should be not less than 0.86; (b) that, upon microscopic observation, the broken-out section should show a cabbage-like appearance with graphite slices taking various directions; and (c) that, upon X ray diffraction, the peak intensity ratio (Ih110/Ih002) between the 002 face (parallel to graphite layers) and 110 face (perpendicular to graphite layers), which serves as an index of the randomness of orientation, should be not less than 0.0050. They retain good qualities of the raw material scaly natural graphite particles and are additionally unique in structure and characteristics. A method of producing such modified particles is also provided. Further provided are secondary cells or batteries in which the modified particles showing good slurry characteristics are used as an electrode material and with which the decrease in discharge capacity is small even at high discharge current values.

A method captures cellular components from a single cell and performs mass spectrometry on the components. The method includes inserting a nanospray ionization capillary tip into a specific region of the cell under observation with a microscope. The nanospray ionization capillary tip can include a filament in the interior. The method further includes capturing the cellular components of the specific region of the cell into the opening of the nanospray ionization capillary tip and keeping the components at the nanospray ionization capillary tip, supplying an ionization supporting solvent from a back-end of the nanospray ionization capillary tip, applying an electric field between a sample inlet of a mass spectrometer and the nanospray ionization capillary tip, whereby nanospray ionization to the cellular components is implemented, and performing the mass spectrometry on the cellular components captured at the nanospray ionization capillary tip.

A microscope system includes an acquisition unit that obtains a specimen image acquired by capturing the specimen stained by an element identification dye that visualizes a predetermined cell constituent element and a molecule target dye that visualizes a predetermined target molecule by using a microscope; a dye amount unit that obtains dye amounts of the element identification dye and the molecule target dye that stain corresponding positions on the specimen for each pixel of the image; an element area identification unit that identifies an area of the cell constituent element based on the dye amount of the element identification dye; a condition setting unit that sets the presence or absence of the predetermined target molecule on the cell constituent element as a condition; and an extraction unit that extracts an area of a target portion that satisfies the condition based on the dye amount of the molecule target dye.

A method for applying gold nanoparticles mimetic enzyme in biological detection adopts gold nanoparticles instead of horse radish peroxidase (HRP) in the biological detection. The detection comprises the following steps: coupling the gold nanoparticles and a specific molecular probe to construct a specific nano-probe; performing specific binding between the nano-probe and the corresponding target molecule to be detected; developing with coloring solution containing peroxide and hydrogen-donating substrate, and measuring absorbance or performing microscopic observation so as to realize the qualitative and quantitative detection of the target molecule. The method belongs to the nanometer material and biomedical nanometer technical field. In the method, the size of the used gold nanoparticles mimetic enzyme is 1-1000nm, the gold nanoparticles mimetic enzyme can imitate HRP to catalyze peroxide and hydrogen-donating substrate to perform a color development reaction, and the enzymatic activity increases with the decrease of the size of the gold nanoparticles. The method of the invention uses the gold nanoparticles to label antibody and other biological molecules to build the similar enzyme labeled antibody and other diagnose preparations, thus having extensive application value.

A microscope stage capable of always heating the entire culture vessel even when the culture vessel is moved in two-dimensional directions, and allowing an object lens and a condenser even in a high-power microscope to approach an object of observation such as a cell until they come into focus. A heating unit (58) faces a well plate (37) on a drive base (49) even when the drive base (49) is driven to any position in two-dimensional directions. Therefore, all the cells A in the small compartments (45) of the well plate (37) are always heated. Since the microscope stage (25) is constituted such that a lower-side base (71) houses an upper-side base (73) and a fixed base (47) in recessed portions, its thickness is considerably small. Accordingly, an object lens (5) and a condenser (3) can approach close to the cells A. Therefore, it is possible for high-power object lens (5) and condenser (3) to focus on the cells A.

Devices, systems and methods for scaling the size and/or position of a marker on a magnified image of an object. In preferred embodiments, the object is an eye that is undergoing laser eye surgery. The eye is viewed through a magnification system or microscope and an image of the eye is presented on a display. One or more markers are present on the image, each identifying a specific target location or landmark on the eye. When a desired magnification setting is selected, the image is scaled accordingly. In addition, one or more of the markers is scaled in size and/or position to reflect the magnification setting. This allows the marker to maintain identification of the target location while reflecting the selected magnification level.

A panel chip for supporting biological samples for observation with an imaging microscope. The glass panel defines a top flat surface, a bottom bearing surface, and at least a few channels extending generally parallel to each other from top to bottom surfaces. Each of the channels defines a top access mouth for ingress of biological. The channels are arranged in subgroups made of a number of unit cells. Instead of being cylindroid, these channels may form cross sectional rectangular or arcuate channels, or cross sectionally water droplet like channels. Each channel has such an inner diameter as to accommodate flow through viscosity of a biological sample containing fluid. The porous substrate that is created by slot channel can provide the maximum binding surface area per unit cell or pixel, as compared to cylindrical pores, thus achieving higher sensitivity and better imaging. Also, this panel chip is capable of being used as direct in situ synthesizing of molecules and drugs. Other impurities in test solutions are however allowed to pass freely therethrough and be least prone to blockage.

Improved assemblies, systems, and methods provide safeguarding against tissue injury during surgical procedures and/or identify nerve damage occurring prior to surgery and/or verify range of motion or attributes of muscle contraction during reconstructive surgery. Such improved assemblies may be utilized in embodiments of methods according to the present invention allowing, preferably by a single hand, manipulation, carrying, parameter adjustment and/or informational feedback generation from within a surgical field. Single-handed operation and easily identifiable feedback generation is desirable especially where focused attention is required, such as during surgery observed through a microscope.

An apparatus and method for selecting and dispensing coverglasses over specimens on slides for the purpose of viewing specimens through a microscope. The selecting device contains a suctioning mechanism for picking up a coverglass from a stack of coverglasses. It also contains the ability to bend the coverglass to assist in separating the coverglasses. The apparatus further contains a matched barrier to eliminate any coverglasses that may stick to the selected coverglass. The selecting device also contains spring members which aid in the dispensing of the coverglass. After the suctioning mechanism releases the coverglass, the spring members exert a force onto the coverglass to insure that it is released from the selecting device and placed onto the slide. After placement of the coverglass onto the slide, capillary action pushes air bubbles out from underneath the coverglass.

The number of seams between magnified images in a created virtual slide is reduced to make the virtual slide clear and sharp. Provided is a microscope apparatus including an objective lens that collects light from a sample on a slide; a focus position detecting section that detects a focus position of the objective lens with respect to the sample; a focus state adjustment section that adjusts a focus state with respect to the sample based on a detection result from the focus position detecting section; and a magnified-image acquisition section that acquires a magnified image of each part of the sample, in which, if the focus position detected by the focus position detecting section is changed by more than a predetermined threshold with respect to a focus state in which an adjacent magnified image was obtained, the focus state adjustment section limits the adjustment in the focus state to the predetermined threshold or less.

An improved microscope stage mount with built-in fiduciary markers is used for fluorescence microscopy, and comprises: (a) an optically-transparent glass plate adapted for specimen mounting and microscope viewing and comprising a specimen mounting area; and (b) a defined and ordered, two-dimensional microscopic array of fiduciary markers, wherein the markers are polymeric pillars affixed to the plate about the specimen mounting area, wherein the markers provide a three-dimensional spatial reference for the specimen.

There is provided a compact image-acquisition optical system which is capable of observing from normal observation to microscopic observation with a single image-acquisition optical system, whose overall length is short, and whose lens outer diameter is small. The invention provides an image-acquisition optical system comprising a plurality of lens groups, wherein, by moving at least one of the plurality of lens groups on the optical axis, it is possible to change a state from a normal observation state (wide-angle end) to a close-up magnified-observation state (telephoto end), and wherein the magnification of the image-acquisition optical system at the telephoto end satisfies β_TELE<−2.0.

The pattern printed sheet 1 of the present invention includes a substrate 2 and a non-visible light-reflective transparent pattern 3 printed on a surface of the substrate, wherein an ink for forming the transparent pattern 3 contains a non-visible light-reflective material capable of selectively reflecting a light having a wavelength in a non-visible light range, and the transparent pattern 3 printed on the surface of the substrate 2 has a multilayer structure in section which is repeated at predetermined intervals as observed by a scanning electron microscope, and reflects only a circular polarization component in a predetermined rotation direction relative to an incident light applied thereto. The pattern printed sheet is usable as a coordinate detecting means which is applicable a data input system of a type capable of directly hand-writing input data on an image screen of a display device, and has a reduced weight and a low price, and is readily obtained in the form of a large area sheet and can be mass-produced.

A closed observational device for an electron microscope is formed of a housing. The housing includes a liquid chamber formed therein, at least one view hole formed at each of a top side thereof and a bottom side thereof and communicating with the liquid chamber and coaxially aligned with the other, and a film mounted to and sealing each of the view holes. Accordingly, a general specimen or a live cell can be placed into the liquid chamber for microscopic observation under the electron microscope. Besides, the present invention can enclose the liquid inside the housing to prevent the liquid from exhausting outward or volatilization.

The invention relates to establishment for a test device and a test method for the bending fracture performance of thin-film materials, in particular to an in situ evaluation system and a method of the mechanical reliability of a layer or a plurality of layers of thin-film materials with the micron to nanometer thickness on a flexible electronic substrate. The system comprises a high-precision micrometer caliper, a balance spring, a translational slide block, a freely supported beam fixed end, a freely supported beam movable end, and the like, wherein a composite beam comprising the micrometer caliper and a flexible substrate exerts precise and controllable step displacement to implement freely supported beam bending experiments; real-time exerted strain corresponding to a freely supported beam span is computed according to parameters and geometrical relationship of the freely supported beam span, sample sizes, and the like; and the bending fracture performance and critical cracking strain of the kind of thin film are tested and evaluated by combining in situ microscopic observation and subsequent scanning electron microscope characterization. The invention does not need to consider the electroconductibility of the thin-film materials, and is still adaptable for non-conductive thin-film materials. The experiment operation is simple and fast, in situ real-time positioning observation and analysis can be carried out on samples.