198 results about "Microscopic observation" patented technology

The invention provides a fracture deformation-seepage cross-scale visualization experiment device and a test method, and relates to the technical field of hydraulic rock mechanics and measurement. Comprising a macroscopic observation assembly, a microscopic observation assembly, a microscopic seepage model, a temperature-stress-seepage loading system, a monochromatic coaxial light source and a data acquisition and analysis system, the microscopic seepage model is used for placing a fractured rock mass; the temperature-stress-seepage loading system is used for injecting fluid with fluorescent microspheres into the microcosmic seepage model, applying load, regulating and controlling temperature and collecting data; the data acquisition and analysis system is used for processing data and images. By arranging the macroscopic observation assembly and the microcosmic observation assembly, double-scale synchronous observation is achieved, the whole process of fracture internal fluid interface evolution and rock mass deformation can be obtained in real time under the multi-physics field coupling conditions of temperature, stress, seepage and the like, visual correlation of deformation, flow and stress is achieved, and the measurement accuracy is improved. And an experimental basis is provided for establishing a fracture multi-field coupling mechanism model.

This invention discloses a method for analyzing the fracture surface of high-Cr steel or alloy steel that cracks in a high-temperature environment. The method involves cutting a metallographic sample at the crack tip of a crack-containing block, polishing it, visually observing the crack morphology, and analyzing the composition of corrosion products on the sample. The sample is then etched with an etchant, and the relationship between the crack direction and the microstructure of the sample is observed under a metallographic microscope to obtain the crack metallographic test results. The remaining crack in the crack-containing block is opened, and the macroscopic features of the fracture surface are visually observed. The microscopic features of different regions of the fracture surface are observed using a scanning electron microscope, and the composition is tested to obtain the micro-region composition. The surface corrosion of the fracture surface is removed using an electrostatic descaling method, and the microscopic features of different regions of the fracture surface are observed under a scanning electron microscope. Based on the crack metallographic test results, the macroscopic and microscopic features of the fracture surface, and the micro-region composition, the cause of the cracking is determined. This invention provides a complete analytical procedure, yields abundant information, and effectively analyzes the causes of component cracking.

According to the living cell observation device, the observation system and the observation method, a light beam generated by an illumination element arranged below a living cell is incident to a pyramid lens array module located above the living cell, the pyramid lens array module reflects the incident light beam at the same direction angle, and the reflected light beam is diffracted into direct light and diffracted light when passing through the living cell; the phase plate causes the direct light and the diffracted light to generate a phase difference, and the direct light and the diffracted light having the phase difference interfere with each other on the imaging element to generate a phase difference image of the living cells. The volume of the observation device is greatly reduced, especially the reduction in the height direction enables the observation device to be easily placed in an existing culture box, real-time and full-life-cycle observation of cells is realized, meanwhile, the pollution of the cells and the change of a culture environment are avoided, and the observation efficiency is improved. The advantages of a current phase difference type microscopic observation system in the aspect of image effects are reserved, and the requirements for precision and stability of a movement mechanism are lowered.

1. Name of the designed product: digital microscope. 2. Use of the designed product: for microscopic observation of microstructure. 3. Design points of the designed product: in shape. 4. Picture or photo best indicating the design points: perspective view.

The utility model discloses a precise gluer, and relates to the technical field of gluers. The device comprises a base with a limiting groove, a limiting check block, a supporting rod, an adjusting arm and a spring clamp which is installed at the front end of the adjusting arm and used for clamping a disposable gluing brush. The supporting rod slides in the limiting groove, and the length of a gluing path is limited through the two limiting check blocks arranged front and back. When the gluing device is used for gluing, the brush head of the disposable gluing brush abuts against the surface of an object to be glued, and the moving path of the supporting rod in the limiting groove is the brushing path of the brush head; the length of glue needing to be coated can be recorded through microscopic observation, the length is converted into the limiting length formed by limiting of the limiting check block on the limiting groove, and after the supporting rod stably slides in the limiting groove, the sliding path is the path of the surface of a glued object coated by the brush head, so that accurate gluing is achieved, and the working efficiency is improved. And dislocation, missed coating, glue leakage and excessive coating of local positions are not easy to occur.

The invention belongs to the technical field of biology, and relates to a bronchoalveolar lavage fluid (BALF) preserving fluid, a kit and a cytological test method. The preservation solution is composed of TCEP, N-acetylcysteine, EDTA.2Na, trehalose, Proclin300, methanol, sodium chloride and HEPES, and all the components have a synergistic effect to achieve sample viscosity removal, oxidation resistance, corrosion prevention, cell protection and pH stabilization. The preserving fluid is suitable for morphological observation and nucleic acid detection of BALF cells, and can stably preserve samples at room temperature and maintain completeness of cell membranes and clear morphological structure. The kit comprises two preparations which are mixed to form a working solution, and the working solution can be directly used for sample preservation on a sampling site. The cytological test method comprises the steps of sample collection, preservation, centrifugal slide preparation, Wright-Giemsa staining and microscopic observation. Experimental results show that preservation solutions of different formulas are compatible with a chromosome system, the cell morphology is kept stable within 3 days, the dyeing effect is good, and it is proved that the preservation system can remarkably improve the preservation stability and detection reliability of BALF samples and is suitable for cytology and molecular diagnosis application of lower respiratory diseases.

The purpose of the present invention is to provide a carbon nanotube dispersion liquid which has excellent time stability in the dispersed state and which is capable of forming a molded article having excellent electrical conductivity, or which is capable of forming a molded article having excellent electrical conductivity. The present invention is a carbon nanotube dispersion liquid or the like that contains a plurality of carbon nanotubes, a solvent, and a thickener, the plurality of carbon nanotubes including single-walled carbon nanotubes, at least a portion of the plurality of carbon nanotubes forming a carbon nanotube bundle, and when the carbon nanotube bundle is observed using a scanning electron microscope, the carbon nanotube bundle has a specific surface area, and the specific surface area of the carbon nanotube bundle is larger than the specific surface area of the carbon nanotube bundle. The average length of the carbon nanotube bundles is greater than 1.50 [mu] m, the average diameter of the carbon nanotube bundles is 18.0 nm or less, the number of carbon nanotube bundles having a diameter greater than 30.0 nm is 15 or less among 100 carbon nanotube bundles, and the average length of the carbon nanotube bundles is 8.0 [mu] m or more when the carbon nanotube bundles are observed with an optical microscope.

The application provides a corrosion agent and method for displaying G20CrNi4 granular bainite structure, the corrosion agent is prepared by 2%-4% nitric acid alcohol solution, hydrochloric acid and ferric chloride in the proportion of 100ml:0.3-0.7ml:0.4g-0.6g; the method for displaying granular bainite structure comprises sample preparation, corrosion agent preparation, granular bainite structure corrosion and metallographic microscope observation. The corrosion agent provided by the application is simple to prepare, the method for displaying granular bainite structure is stable and reliable, simple to operate, and can realize corrosion and clear display of granular bainite structure of carburizing bearing steel.

The present application belongs to the technical field of petroleum geological exploration and analysis, and particularly relates to a single-period oil inclusion composition analysis method based on crude oil inclusions. The present application combines fluorescence microscopic observation and quantitative fluorescence analysis methods, can accurately determine the diameter of oil inclusions, determine the appropriate grinding mesh, and identify the period of oil inclusions in the microscopic observation stage, and provides clear basis for the subsequent experimental process. The analysis of the present application covers the differential processing method of oil inclusions in calcite and quartz minerals, can efficiently and completely perform group composition pretreatment on oil inclusion-containing particles, is convenient to operate, has good reproducibility, and provides reliable technical support for revealing the oil and gas charging history and restoring the reservoir forming process. The present application adopts a crushing device equipped with a low-cost, large-capacity closed zirconium oxide grinding tank, realizes synchronous crushing of multiple samples on the basis of optimizing experimental equipment, and significantly improves the sample processing flux and experimental efficiency.

The invention provides a material surface and interface damage in-situ characterization system for multi-factor coupling corrosion, and relates to the technical field of in-situ detection equipment. The technical problem that in the prior art, in-situ microscopic observation cannot be conducted on a material surface interface in a simulated service environment, and the dynamic evolution rule of surface interface corrosion behaviors in the same area in the corrosion process cannot be accurately tracked is solved. According to the material surface and interface damage in-situ characterization system for multi-factor coupling corrosion, simulation of heat, salt and force multi-field coupling corrosion is achieved through a salt mist generating device, a multi-point heating module and a corrosion-resistant loading table, an X-ray diffractometer is allowed to conduct in-situ diffraction testing through an X-ray incident window and an X-ray emergent window, corrosion product in-situ phase analysis under the simulated working condition is achieved, and in-situ phase analysis of the corrosion product under the simulated working condition is achieved. And the corrosion-resistant loading table can enter the electron microscope for micro-area structure and micro-area component analysis, the multi-point heating module can keep the consistency of the sample state in the X-ray diffractometer and the electron microscope, and different heating sites can be used for heat, salt and force environment experiments.

The present invention relates to a method for correcting a spherochromatic aberration that occurs when examining a sample with a microscope. The microscope comprises an objective lens and a corrective device arranged in a beam path of the microscope for correcting a spherical aberration. According to the method, data for describing the spherical aberration as a function of a wavelength of light imaged by the microscope are provided. A sample is examined with the microscope, and at least one spectral range of the light imaged by the microscope is determined.A control parameter of the correction device is determined based on the provided data describing the spherical aberration as a function of wavelength for the at least one determined spectral range, in order to minimize the spherical aberration for the at least one determined spectral range. The correction device is then set to the determined control parameter. The invention further relates to a microscope, which is in particular a light microscope.

1. Name of the designed product: portable operating microscope. 2. Use of the designed product: for operating microscopic observation. 3. Design points of the designed product: in shape. 4. Picture or photo best indicating the design points: design 1 perspective view. 5. Design 1 is designated as the basic design.

The invention discloses a high-power microscopic chip mounter which comprises a base, a supporting frame and a carrying table are arranged on the base, a high-power microscope lens and an industrial camera are arranged on the supporting frame, the high-power microscope lens is provided with a plurality of objective lenses capable of being rotatably switched, and the industrial camera is connected with the opposite sides of the objective lenses of the high-power microscope lens; a vacuum transparent suction nozzle is movably arranged on the side edge of the support frame, the vacuum transparent suction nozzle is used for adsorbing a chip, and an objective lens of the high-power microscope lens is positioned above a transparent part of the vacuum transparent suction nozzle; the industrial camera can collect a front image of a substrate located on the carrying table and displayed in an objective lens of the high-power microscope lens and a front image of a chip adsorbed by the vacuum transparent suction nozzle. According to the invention, the objective lens end of the high-power microscope lens capable of rotating and switching different multiplying powers observes the substrate and the patch through the vacuum transparent suction nozzle, so that the high-power microscopic observation of the substrate and the patch is realized, the structure of the chip mounter is simplified, and the production efficiency is improved.

This invention relates to a method for assembling and adjusting a holographic concave grating monochromator, belonging to the field of optical instrument assembly and adjustment technology. It solves the problems of existing monochromator assembly and adjustment methods being cumbersome, time-consuming, labor-intensive, or having high theoretical requirements and low efficiency. The method involves leveling the holographic concave grating monochromator and laser; recording the initial spot height of the laser; directing the laser perpendicularly to the entrance slit of the monochromator to the center of the grating; measuring and recording the heights of the 0th and ±1st order diffraction spots; plotting the trajectories of the multi-order spectral spots; adjusting the pitch and / or tilt angle of the grating according to the trajectory until the center of each diffraction spot is collinear with the initial spot height; rotating the grating to allow the 0th order light to exit from the exit slit; using a right-angle fixture to adjust the grating shaft lever to be perpendicular to the drive screw and locking it; placing an external light source at the entrance slit; observing the clarity of the spectral lines at the exit slit using a microscope; and adjusting the thickness of the shim at the exit slit to achieve precise conjugate matching between the entrance and exit slits. This invention is simple to operate, quick to assemble and adjust, and highly accurate.

The application relates to the technical field of microscopes, and discloses an adjustable fluorescence microscope which comprises a base, a mirror arm arranged on the top of the base, and a sample platform arranged on one side of the mirror arm; the top surface of the base is provided with a lifting device; and one end of the sample platform is movably connected with the lifting device. The fluorescence microscope controls the sample platform to move downwards through the lifting device; a first filter automatically turns over around a fixed shaft to the outside of the sample platform under the action of gravity, thereby automatically providing a horizontal operation space for the sample platform; after sample replacement is completed, the sample platform only needs to be lifted to a preset observation position; when the sample is replaced, the sample platform only needs to be controlled to move, manual operation of the filter is not required, so that the sample can be replaced by the staff in a high-frequency sample replacement scene, the human time cost for sample detection is shortened, and the overall working efficiency and operation convenience of the microscopic observation system are improved.

The utility model discloses a cell medicine handles microscopical observation special culture dish belongs to culture dish technical field. Including dish body and dish cover, dish cover covers the top surface of dish body, the inside installation of dish body has cross bulkhead, and cross bulkhead divides the space in dish body into four groups of culture cavities, and the top surface of dish cover is provided with four groups of through -holes, and four groups of through -holes and four groups of culture cavities correspond one -to -one, and the bottom surface of through -hole all has the through -pipe that communicates, and the inside installation of through -pipe all has rubber plug, and the center of rubber plug is provided with liquid injection hole, this technical scheme divides the space into four groups of independent culture cavities through the cross bulkhead in dish body, and cooperates the through -hole, through -pipe and rubber plug with liquid injection hole of corresponding one -to -one on dish cover, effectively solved the problem that the traditional single cavity culture dish needs to use multiple culture dishes in multiple drug concentration gradient experiments, resulting in the problems of large consumable consumption, poor consistency between groups, complicated operation and high error risk.

The invention relates to the technical field of radioactive waste treatment, and provides a metal solidified body analysis method which comprises the following steps: acquiring a plurality of samples from a plurality of set positions of a metal solidified body; part of the sample is dissolved to prepare an analysis solution, and the other part of the sample is prepared to form an observation surface; obtaining the concentration of a target component in each analysis solution, wherein the target component comprises a zirconium element; based on the concentration of the target component in each analysis solution, obtaining the average concentration and data discrete index of the target component; performing microscopic observation on each observation surface to obtain the volume percentage of a Fe-Zr intermetallic compound phase and the volume percentage of an iron-based solid solution phase in each sample with the observation surface; it is determined whether the volume percent of the Fe-Zr intermetallic compound phase satisfies a first threshold range and whether the volume percent of the iron-based solid solution phase satisfies a second threshold range. By analyzing the target components, the Fe-Zr intermetallic compound phase and the iron-based solid solution phase, the performance of the metal solidified body product is verified.

The invention discloses a biological tissue sample preparation sleeve solution, and belongs to the technical field of biological sample preparation. The stationary liquid comprises: 8-12% of an aldehyde cross-linking agent; 1-3% of a phosphate buffer system; and the balance of purified water. The dehydration liquid comprises the following components: 55-70% of an alcohol solvent; 10 to 20 percent of isopropanol; 10-20% of an ester organic solvent; 0.5 to 2 percent of a non-ionic surface active agent; the transparent liquid comprises the following components: 50-70% of a polyol compound; 10-20% of an ether cosolvent; 10 to 30 percent of isopropanol; the refractive index of the transparent liquid is 1.47 to 1.52; the cleaning solution comprises the following components: 60-85% of a polyol solvent; 10-20% of an ether solvent; 1-3% of an anionic surfactant; 0.1-1% of a complexing agent; and the balance of purified water. Tissue shrinkage is effectively reduced, the cell structure is kept complete, the transparency effect is improved, and microscopic observation and dyeing analysis are facilitated.

The invention discloses an exocytosis protein subcellular localization method for mannitol-induced plasm-wall separation, and relates to the technical field of cytobiology, the method comprises the following specific steps: constructing a fusion expression vector: connecting a plant target exocytosis protein gene with a fluorescent protein gene through a gene cloning technology to obtain a fusion expression vector; inserting into a plant expression vector to obtain a recombinant expression vector containing the target extracellular secretory protein-fluorescent protein fusion gene; performing bacterial colony PCR identification and sequencing verification on the recombinant expression vector; by adopting an agrobacterium tumefaciens-mediated plant leaf instantaneous transformation technology, the fusion gene is rapidly expressed in plant cells, subsequent experiments can be carried out after culture is carried out for 2-3 days, the experiment period is greatly shortened, meanwhile, leaf cells are induced to be subjected to plasmon-wall separation through a gradient mannitol solution, positive cell screening and identification are not needed, and the application prospect is wide. And positioning can be realized by directly observing fluorescence signal distribution by using a laser confocal microscope.

The invention discloses a mudstone interlayer high-pressure grouting test device and a grouting diffusion and reinforcement mechanism test method, the device comprises a constant-speed constant-pressure injection pump, an intermediate container, a constant-temperature injection cabin and a data acquisition system, and the inlet end of the constant-speed constant-pressure injection pump is connected with an injection pressure sensor; the intermediate container comprises a fluid container and a slurry container, an inlet end of the constant-temperature injection cabin is connected with an inlet pressure sensor, a cavity of the constant-temperature injection cabin is used for placing a mudstone sample, and a heating and temperature control unit is arranged; the two ends of the mudstone sample are connected with the micro-seismic monitoring device, and the micro-seismic monitoring device comprises at least one group of micro-seismic sensors. The device can accurately control the rate and pressure of grouting fluid, capture microseismic signals during slurry diffusion or crack expansion in real time through the microseismic monitoring sensor, and can quantitatively analyze high-pressure grouting diffusion and reinforcement mechanisms in combination with chemical reaction analysis and microscopic observation, so that the research precision and reliability are improved, and the research cost is reduced. And a method reference is provided for research of similar geological engineering problems.

The invention relates to the technical field of biological flaking, and particularly discloses a grading rapid flaking method for high-flux sporopollen analysis, and the method comprises the following steps: collecting pollen samples of a plurality of modern plants, carrying out drying pretreatment on the pollen samples, carrying out primary flaking on the dried and pretreated pollen samples, and carrying out microscope observation; screening based on the result of the first microscopic observation, and mixing at least two target samples with significant morphological difference to form a mixed sample; according to the method, the mixed sample is subjected to acetic anhydride decomposition treatment, and the mixed sample subjected to acetic anhydride decomposition treatment is subjected to secondary flaking and microscope observation, so that when a large number of modern pollen samples are treated, the flaking efficiency can be effectively improved, and meanwhile, a high-definition pollen form micrograph is ensured.

The utility model discloses a kind of microbial microscopic observation auxiliary devices, it is related to the technical field of auxiliary tool for microbial observation, including microscope body for observing microorganism, the outside of microscope body is provided with dust cover for dust prevention, the left and right sides of dust cover are respectively fixedly installed with the storage rack one and storage rack two for receiving experimental apparatus, the top of storage rack two has the storage cavity for placing experimental apparatus, the dust cover is made of transparent material, the dust cover is rectangular structure.The utility model said a kind of microbial microscopic observation auxiliary device, by the effect of handle one, dust cover can be placed in the outside of microscope body, realize dust prevention, solve the microorganism microscope in prior art is not used for a long time and is exposed to the outside world, leading to dust adheres to its surface, use convenient, and by the effect of non-slip pad, can play the effect of antiskid, ensure the stability of the auxiliary device placed.

The utility model relates to the technical field of bioengineering, and provides an array chip integrating impedance measurement and microscopic observation, which comprises a transparent substrate, a circuit board, a 3D printing board and an electrode unit, and one side of the transparent substrate is provided with a conductive film; the circuit board is arranged on the conductive film, and a plurality of micro-channels are arranged on the circuit board in an array manner; the 3D printing plate is arranged on the side, away from the conductive film, of the circuit board, a plurality of micro-cavities are arranged on the 3D printing plate in an array mode, and the positions of the micro-cavities correspond to the positions of the micro-channels one to one; the electrode unit comprises a first electrode plate and a second electrode plate, the 3D printing plate is covered with the first electrode plate, the circuit board is surrounded by the second electrode plate, and the second electrode plate and the first electrode plate form a closed loop through a conductive film. Through innovative fusion of micromachining and bioelectrochemistry, synchronization of electrophysiology (impedance) and morphology (microscopy) is realized, and the dynamic growth process of organisms is conveniently and continuously monitored.

The application discloses a method for evaluating the scratch resistance of materials, and belongs to the technical field of material performance detection. The method comprises the following steps: under a preset friction condition simulating a daily use scene, a hard abrasive particle-containing abrasive is used to apply a friction action to a surface of a to-be-tested base material to form a scratch area; a gray-scale image containing the scratch area is obtained by using an optical imaging system; and based on the image, a quantitative characterization parameter for characterizing the scratch resistance of the to-be-tested base material is calculated. Through real abrasive particle scratch simulation, automatic imaging and a quantitative algorithm associated with perception, the application realizes objective and repeatable quantitative evaluation of the scratch resistance of hard materials such as glass and metal, effectively overcomes the problem that traditional hardness testing or manual microscopic observation is disconnected with actual user experience, and is suitable for material research and development and quality control of consumer electronic products.

The present application relates to the technical field of waxy crude oil pipeline transportation and storage, and particularly relates to a method for determining the effective diffusion coefficient of wax molecules, comprising the following steps: S1, establishing a calculation model of the effective diffusion coefficient of wax molecules in wax deposits; S2, obtaining multiple wax crystal microscopic images of the wax deposit sample at different temperatures through a microscopic observation device; S3, performing image preprocessing on each wax crystal microscopic image, and combining the preprocessed wax crystal microscopic images, the calculation model of step S1 is used to calculate the effective diffusion coefficient of wax molecules in the wax deposit sample in each wax crystal microscopic image; S4, fitting the effective diffusion coefficient of wax molecules in the wax deposit sample in each wax crystal microscopic image, and obtaining the change relationship between the ratio of the effective diffusion coefficient of wax molecules in the wax deposit and the diffusion coefficient of wax molecules in the crude oil and the solid-phase wax crystal particle concentration. The method for determining the effective diffusion coefficient of wax molecules can accurately calculate the effective diffusion coefficient of wax molecules.

The utility model provides a kind of microscope external optical fiber lighting device, it is related to microscope lighting device technical field, including optical fiber, the one end of optical fiber corresponds with light source, and the other end extends to the light path entrance of the illumination system of microscope;The illumination system is sequentially provided with variable aperture diaphragm, condensing system and objective along light path propagation direction, and the aperture size of variable aperture diaphragm is changed to control the numerical aperture of illumination;The light emitted by light source is transmitted to variable aperture diaphragm by optical fiber, and clear micrograph is formed by condensing system, objective.The utility model has the beneficial effect of not only breaking through the constraint of traditional built-in illumination in physical space, and heat source can be completely isolated in microscope outside, avoid the occupation of light source to microscope internal space and the influence of cooling fan vibration on microscopic observation, in addition, different microscopes can be matched by different light sources, with flexible adaptive expansibility, can be widely applied to various microscopic observation systems.

The invention belongs to the technical field of insect collectors, and particularly relates to a portable insect collector with a replaceable collecting device. The device comprises a collection host, a collection head, a negative pressure generator, a threaded connection interface, a collection device and a guide hopper. The acquisition host is in a transparent tubular shape; the collection head is sleeved on the top of the collection host; the negative pressure generator is inserted and mounted on the outer wall of the acquisition host; the threaded connection interface is fixedly mounted at the bottom of the collection host; the collection device is mounted in the threaded connection interface in a butt joint manner; through the modular concept of one host and multiple slave tubes, a user can quickly switch the collection terminal according to target insects and subsequent requirements in the field, multiple functions such as living body observation, specimen poisoning, microscopic observation and sample screening are achieved, portability and functionality are achieved, the insect escape rate and the damage rate are greatly reduced, and the cost is reduced. And seamless connection from acquisition to processing is realized.

The invention discloses staining agent uniform smearing equipment for a clinical laboratory, and belongs to the technical field of medical instruments, the staining agent uniform smearing equipment comprises an automatic smear conveying belt, the automatic smear conveying belt is provided with a plurality of smear placing mechanisms, each smear placing mechanism is provided with a bubble left-right swing removing mechanism, and the top of the automatic smear conveying belt is provided with a uniform smearing mechanism. And a bubble rolling removal mechanism is arranged on the automatic smear conveying belt. The bubble left-right swing removing mechanism works to push deep bubbles to the surface, the bubble rolling removing mechanism thoroughly discharges surface bubbles and corner-containing tiny bubbles, and for areas such as four corners and the edge of a smear where the bubble left-right swing removing mechanism cannot reach, due to the fact that stress on the edge of the smear is weak during swinging, tiny bubbles are prone to remaining, and the smear is not prone to falling off. The bubbles at the corners can be thoroughly removed through rolling coverage of the driving wheel, so that the surface of the smear is not blocked by any bubble, the situation of misjudgment of cell vacuoles cannot occur during subsequent microscope observation, and the blood cell morphology recognition accuracy is improved.

This invention relates to the field of microscopic observation technology, specifically to an extended depth-of-focus microscopic imaging system incorporating optical tweezers functionality. The system includes a beam generation and combining module, a signal acquisition module, a signal separation module, an image reconstruction module, and an optical tweezers analysis module. The system generates a phase-modulated extended depth-of-focus illumination beam and an optical tweezers manipulation beam, which are then spatially coaxially combined to form a combined beam, which is then guided into the microscope objective to act on the sample region. The acquired optical signal is divided into independent detection channels through beam splitting processing. The imaging channel, combined with a depth-of-field extension point spread function model, optimizes the fusion algorithm to reconstruct the three-dimensional structure of the sample. The optical tweezers monitoring channel analyzes the optical path signal, calculates the three-dimensional position of particles, trapping stiffness, and optical force, and visualizes the three-dimensional relative relationship between these two parameters by combining the imaging results. This scheme achieves integrated design of the imaging optical path and the optical tweezers optical path, optimizes image reconstruction quality under large depth of field, and enables synchronous and collaborative operation of microscopic imaging and optical manipulation.