The invention relates to a device and a method for detecting the retention performance of a liquid-phase solute of
articular cartilage. The method comprises the following steps: adhering and fixing a treated
cartilage on a perforated rubber plug; the
cartilage pasted with the rubber plug is placed in a retention device with a perforated plate at the bottom, and water is added till the
cartilage completely exceeds an overflow opening; the retention pressing rod provided with the rubber plug is pressed into the retention device, and whether liquid leakage exists or not is detected in advance; discharging the water, adding a solution required by a retention experiment, and pressing the retention pressure rod again; the retention device is arranged in the cylindrical base, and the screws on the two sides are fastened; the base of the device is fixed on a lower disc of a testing
machine; a retention compression bar of the device is fixed on an upper disc of a testing
machine; and setting expected pressure and time, starting to load experimental pressure, and then starting a cartilage retention experiment. And after the retention experiment is finished, observing the retention state of the fluorescent solute molecules by using a
slicing machine and a
laser confocal microscope. The cartilage
liquid phase solute retention performance is effectively detected, the problem that sealing performance is difficult in a retention experiment is solved, a cartilage
mass transfer research method is expanded, and the detection precision and stability are improved. The method is expected to be popularized to become a
standard test technology of cartilage retention performance, and plays a positive role in promoting cartilage in-vitro experiments.