648 results about "Horse radish peroxidase" patented technology

A method for applying gold nanoparticles mimetic enzyme in biological detection adopts gold nanoparticles instead of horse radish peroxidase (HRP) in the biological detection. The detection comprises the following steps: coupling the gold nanoparticles and a specific molecular probe to construct a specific nano-probe; performing specific binding between the nano-probe and the corresponding target molecule to be detected; developing with coloring solution containing peroxide and hydrogen-donating substrate, and measuring absorbance or performing microscopic observation so as to realize the qualitative and quantitative detection of the target molecule. The method belongs to the nanometer material and biomedical nanometer technical field. In the method, the size of the used gold nanoparticles mimetic enzyme is 1-1000nm, the gold nanoparticles mimetic enzyme can imitate HRP to catalyze peroxide and hydrogen-donating substrate to perform a color development reaction, and the enzymatic activity increases with the decrease of the size of the gold nanoparticles. The method of the invention uses the gold nanoparticles to label antibody and other biological molecules to build the similar enzyme labeled antibody and other diagnose preparations, thus having extensive application value.

The invention relates to a magnetic gamma-Fe2O3 nano-particle mimetic enzyme and provides a method for applying a magnetic gamma-Fe2O3 nano-particle mimetic enzyme to biological detection. The magnetic gamma-Fe2O3 nano-particle mimetic enzyme replaces a horse radish peroxidase (HRP) to be used in biological detection. The method comprises the following steps: coupling carboxyl groups on the surface of magnetic gamma-Fe2O3 nano-particles and a specific molecular probe to construct a specific nano probe; combining the nano probe with the specificity of the corresponding target molecules to be detected; developing by using a developer containing peroxides and hydrogen-supply zymolytes; and measuring the absorbency or carrying out the micro-observation so as to realize the qualitative and quantitative detection on target molecules.

The invention discloses a bio-medical adhesive, which can be cross-linked with a wound in situ, and a preparation method of the bio-medical adhesive. The preparation method is characterized by comprising the steps of first, preparing oxidized sodium alginate by using sodium periodate to oxidize sodium alginate; dissolving the oxidized sodium alginate in an MES (Methyl Ester Sulfonate) buffer solution, dissolving 1-(3-dimethyl aminopropyl)-3-ethyl carbodiimide hydrochloride and N-hydroxyl succinimide in the MES buffer solution as well under nitrogen protection, continuously adding dopamine, carrying out dialyzing and freeze-drying after reacting for 8 to 24 hours, and obtaining A liquid by dissolving a reaction product in a PBS (Phosphate Buffer Solution) or a sodium borate solution; then, dissolving I-shaped collagen in an acid solution, and obtaining B liquid by neutralizing the solution pH (potential of Hydrogen) to be 5 to 8; firstly pre-treating the wound by using hydrogen peroxide or HRP (Horse Radish Peroxidase) before the bio-medical adhesive is used, then immediately coating the wound with the bio-medical adhesive after mixing the A liquid with the B liquid, and forming gel in 30 to 120 s, wherein the gel is firmly adhered to the surface of the wound. The bio-medical adhesive disclosed by the invention has a stronger adhesive force under a moist environment in vivo, integrates filling, sealing and bleeding stopping functions, is good in biocompatibility, is bio-degradable and can be used for promoting wound healing.

The invention discloses a chemiluminescence reagent, and relates to a chemiluminescence reagent which is mainly applied to a biotechnology, bioscience, medical diagnosis, food safety, and environment-friendly detection. The chemiluminescence reagent is prepared from the materials by mass percent: at least one 0.001-0.1% luminous reagent, at least one 0.001-0.2% oxidant, at least one 0.01-0.5% luminous enhancer, at least one 0.000001-0.05% background control agent, and a buffer solution of which the pH value is 8-11. The key is as follows: chemiluminescence is adjusted by utilizing the background control agent (particularly the antioxidant); the chemiluminescence reagent is valid on background control of enhanced chemiluminescence enzyme immunoassay detection, improvement of the signal to noise ratio and the like. The basis is how to control luminol free radical and concentration of related free radical in a horse radish peroxidase-luminol luminescence system.

The invention discloses a preparation method of a magnetic sandwich nano immunosensor and application of the preparation method. The preparation method is characterized by comprising the following steps: mixing and stirring a collaurum solution and silane ferriferrous oxide into a colorless and transparent solution, and carrying out magnetic separation by externally applying a magnet to obtain FE3O4/Au colloid nano magnetic beads; loading horse radish peroxidase (HRP) and secondary antibodies of an object to be measured to the FE3O4/Au colloid nano magnetic beads to obtain a secondary antibody probe Fe3O4/Au-HRP-Ab2; electrically depositing Au to a glassy carbon electrode, loading primary antibodies of the object to be measured, and then closing non-specific active sites by using protein to obtain an Abl/Au/GCE electrode; and finally, loading antigen to the Abl/Au/GCE electrode, and then obtaining the sandwich nano immunosensor by combining the magnet probe. The magnetic sandwich nano immunosensor can be used for measuring the concentration of melamine, tonyred, clenbuterol or estrogen in food and has the advantages of high detection speed, high accuracy and sensitivity and low cost.

The invention discloses a kit for detecting the estradiol (E2) by utilizing magnetic particle chemiluminescence immunoassay. The kit comprises estradiol series calibrator, magnetic particle solution enveloped by the second antibody, estradiol marked by horse radish peroxidase, estradiol antibody, chemiluminescence substrate liquid and concentrated washing liquid. The invention further discloses apreparation method of the kit. The kit of the invention can quantitively detect the content of estradiol in the serum and blood plasma samples of human body, and can simultaneously detect a large number of samples, thus having advantages of simpleness, convenience, rapidness, stability, high sensitivity and the like, and providing a extremely valuable detection method for clinical diagnosis and scientific research.

The invention discloses a quantitative detection kit combining magnetic particles with chemiluminescence immunoassay for beta human chorionic gonadotropin (beta-hCG). The kit comprises beta-hCG calibrators, magnetic particle suspension coupled with streptavidin, a beta-hCG antibody labeled with biotin, a beta-hCG abzyme combination, a beta-hCG quality controller, chemiluminescence liquor A, chemiluminescence liquor B, 20 times concentrated washing liquor, and a reaction tube, wherein enzyme adopted by the beta-hCG abzyme combination is horse radish peroxidase with the purity RZ being more than or equal to 3.0 and the activity being more than or equal to 250U/ml. The invention also discloses a preparation method of the kit. Compared with the conventional kit, the quantitative detection kit is simple and convenient to operate, is safe, does not cause environment pollution, and also has the advantages of wide concentration range, low cost, good stability and the like of detection samples.

The invention discloses an aptamer-based Sandwich ELASA method for detecting nervous necrosis virus infection of groupers. The method comprises the following steps: fixing a biotin-labeled aptamer onto a pore plate pre-coated with streptavidin; capturing CP proteins in a to-be-detected sample by the fixed aptamer, and combining the CP proteins by the biotin-labeled aptamer; adding horse radish peroxidase-streptavidin for incubating and combining, developing by a horse radish peroxidase developing kit, and analyzing whether NNV virus infection exists according to light absorption value change. According to the invention, by utilizing the characteristics of high affinity and high specificity of the aptamer, a Sandwich ELASA detection method based on aptamer detection is established and can be used for detecting nervous necrosis viruses during grouper culture. Compared with the traditional ELISA and other detection methods, the method disclosed by the invention has the characteristics of high detection speed, high sensitivity and specificity, low cost and high simplicity in operation.

The invention discloses a kit for chemiluminescence immunity quantitative detection of an MYO nano magnetic particle. The kit comprises an MYO calibrator, a nano magnetic particle suspension liquid coupled with streptavidin, bioti-labeled MYO antibodies, MYO abzyme conjugate, an MYO quality control product, a chemiluminescence liquid A and a chemiluminescence liquid B, a 20-time concentrated washing liquor and a reaction tube, wherein for the MYO abzyme conjugate, used enzyme adopts horse radish peroxidase with purity RZ larger than or equal to 3.0 and activity larger than or equal to 250 U/mL. Besides, the invention further discloses a preparation method of the kit. Compared with the conventional kit, the kit provided by the invention has the advantages of high sensibility and test automation, wide measurable concentration range, long useful life of a reagent, simple operation and the like.

The invention discloses a toxoplasma gondii IgM antibody detection kit combined with the FITC-anti-FITC indirect coating technology and the chemiluminescent immunoassay technology, and a preparation method thereof. The kit of the invention is composed of a negative control, a positive control, solid-phase vectors for anti-FITC antibodies, anti-human Mu-chain monoclonal antibodies of FITC markers, toxoplasma gondii antigens which are marked by horse radish peroxidase, chemiluminescent substrates and concentrated washing solutions. The kit of the invention can be used as the aided detection index for prenatal prepotency diagnosis, and has vital significances for improving the birth population quality and doing the family planning and the prepotency well.

The invention relates to a biosensor based on nitrogen-hybridized mesoporous carbon as well as a preparation method and application of the biosensor. The biosensor comprises a working electrode, wherein the working electrode comprises a glassy carbon electrode; the surface of the detecting end of the glassy carbon electrode is sequentially connected with an L-cysteine, nitrogen-hybridized mesoporous carbon, a gold nanoparticle film and a sulfydryl-modified capture probe; the gold nanoparticle film is also connected with mercaptoethannol. The invention also provides an application of the biosensor. The application comprises the following steps: dropwise adding an object chain contained liquid to be detected and a signal probe contained liquid on the surface of the working electrode; dropwise adding a nanogold cluster labeled horse radish peroxidase-streptavidin contained buffer solution; and measuring in an electrolytic tank with hydroquinone and hydrogen peroxide as substrates, wherein the electrolytic tank is connected with a three-electrode system. The biosensor is high in sensitivity, rapid in response, high in detecting precision, relatively strong in anti-interference, simple and convenient in application operation, high in efficiency and low in detecting cost.

The invention discloses a preparation method of an electrochemical chlordimeform sensor based on a composite cerium-doped porous nanocomposite, and belongs to the technical field of novel nano functional materials and biosensors. The method comprises the steps that the novel two-dimensional nanocomposite Ce-MoO3/TiO2@g-C3N4 is prepared firstly, and by means of the good biocompatibility and large specific surface area of the material, the material is loaded with a chlordimeform antibody; horse radish peroxidase is fixed by means of the crosslinking action of glutaraldehyde; when detection is conducted, the horse radish peroxidase can catalyze hydrogen peroxide to generate an electrochemical signal, and by means of the influence of specific quantitative combination of the antibody and an antigen on electron transmission capability, the current intensity is correspondingly decreased, and the electrochemical biosensor which is low in cost, high in sensitivity, good in specificity, rapid in detection and easy to prepare is finally prepared for detecting chlordimeform.

The invention relates to a diagnostic reagent kit for testing the hepatitis c virus (HCV) and the preparation and test method, which is to add the HCV recombinant antigen used for peridium into the buffer solution, blend it, move into the luminous microplate, make incubation for 18 hours under 4DEG.C, wash the luminous microplate, add into the confining liquid, leave the liquid after incubation and fully dry the luminous microplate to complete the preparation of the pre-peridium luminous microplate; combine the anti-human IgG used for marking and the horse radish peroxidase by improving the sodium periodate to complete the preparation of the enzyme marker; prepare the chemical luminous substrate solution A with luminal, Tween20 and luminous intensifier and prepare the chemical luminous substrate solution B with the hydrogen peroxide. The reagent kit also comprises the sample diluent and concentrated scrub solution. The negative corresponds to the normal human serum while the positive corresponds to the people with serum of pooled serum with HCV antibody. The reagent kit provided in the invention has much higher detection sensitivity than the ELISA, which is safe and reliable, easy to operate with low cost, and without any expensive full-automatic chemical luminous measuring apparatus required.

The invention discloses a biological medical adhesive capable of producing in situ crosslinking with a wound and a preparation method of the adhesive.The adhesive is prepared with the following method: firstly, sodium periodate oxidative sodium carboxymethylcellulose-dopamine is used for preparing dialdehyde sodium carboxymethylcellulose, a product is dissolved in an MES buffering solution after dialysis and drying, then a certain amount of 1(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-H-hydroxysuccinimide are dissolved in the MES buffering solution as well, stirring reaction is performed by 10-40 min, dopamine is added then, stirring reaction is performed by 8-24h under nitrogen protection, and dialysis, lyophilization and vacuum preservation are performed.Before using, hydrogen peroxide or horse radish peroxidase is used for pretreating the wound, then the wound is coated with the lyophilized adhesive after the lyophilized adhesive is dissolved in a PBS buffering solution, and the wound can be bonded within 2-10 min.The biological adhesive has higher bonding strength under a moist environment in a body, the biocompatibility is good, and the adhesive is biodegradable and capable of promoting wound healing.

The invention discloses preparation and detection methods of a screen-printed electrode immunosensor for rapidly detecting microcystin, and belongs to the field of environment monitoring technologies. Through a magnetic field formed by a magnet below a screen-printed electrode, core-shell magnetic nanoparticles Fe3O4@Au are fixed on the surface of a working electrode; through the adsorption between nano-Au and a microcystin antibody, the microcystin antibody is fixed on the surface of the electrode to prepare an MCLR antibody electrode; a certain concentration of MCLR and MCLR which is marked by horse radish peroxidase are together modified on the surface of the working electrode; after immunoreaction is performed for a period of time, a peak current value is measured by using DPV (differential pulse voltammetry) to obtain standard curves of the MCLR and the oxidation peak current; the steps are repeatedly carried out on water samples to be detected, and the obtained oxidation peak currents are compared with standard curves to obtain the MCLR concentration. According to the preparation and detection methods of the screen-printed electrode immunosensor for rapidly detecting microcystin, benefit is brought to rapidly detect the MCLR, the water samples do not need purification treatment, simplicity and rapidness are achieved, and the detection cost is reduced.

The invention belongs to the field of detecting a trace amount of ions in the water environment, and particularly relates to a visualization method for rapidly detecting a trace amount of uranyl ions in the water environment. The method mainly includes the steps that DNAzyme with the specific recognition function on UO2 <2+> is fixed to the surfaces of magnetic beads, and horse radish peroxidase is preassembled on the surface of nano-gold; then the magnetic beads are connected with the nano-gold through the cutting effect of the UO2<2+> on the DNAzyme and the hybridization reaction of DNA, after separation and collection are carried out through an external magnetic field, H2O2 oxidation tetramethyl benzidine is efficiently catalyzed through the horse radish peroxidase to enable a solution to be changed from the blank to the blue, and therefore sensitive and specific visualization rapid detection of the UO2<2+> ions is achieved. As the method has the advantages of being high in sensitivity, high in specificity, high in matrix interference resistance, simple, rapid, low in cost and the like, the method can be used for site rapid visualization detection of the trace amount of UO2<2+> ions in various water samples.

The invention discloses an on-site detection immuno-chip, comprising a chip carrier and an agarose gel layer which is paved on the chip carrier, wherein, the agarose gel layer is fixed with a plurality of antibody microarrays of 4*4, and chip-dedicated fences or Super PAP Pen scribings are used for separating the microarrays from each other; the double antibody sandwich principle is adopted to detect the antigen to carry out the dot matrix of corresponding capture antibody, positive control and negative control on the solid phase carrier simultaneously; the antibody protein is connected with the solid phase carrier through the covalent bond and physical adsorption; the sample liquid to be detected and the chip are directly incubated; the antigen to be detected in the samples combine with the corresponding antibody which is fixed on the chip; a specific monoclonal antibody probe marked by horse radish peroxidase is added and the macroscopic detection results can be obtained after the coloration of substrates. The invention can detect viruses in multiple aquatic animal samples and the results are macroscopic, thus being applicable to rapid and accurate detection of viruses of aquatic animals in breeding production.

The invention discloses a copper ion detection kit based on click chemistry and G tetramer with HRP (Horse Radish Peroxidase) activity and a detection method of the copper ion detection kit. A G tetramer formation sequence is formed by two DNA (deoxyribonucleic acid) sequences modified with an azide group and an alkynyl group through click chemistry in a reaction system, and then ferriheme and KCl are added to form a ferriheme/G tetramer structure. The ferriheme/G tetramer has the HRP activity and can catalyze a colourless substrate TMB (tetramethylbenzidine) to form a colored substrate, and the experiment result can be judged by eyes. When quantitative analysis is performed, the final reaction solution is transferred to an elisa plate, and the OD (optical density) value of the solution under 450nm can be read by an elisa meter. The copper ion detection kit can be used for performing qualitative and quantitative analysis of concentration of Cu<2+>, the detection system is simple, the specificity is good, the detection is not affected by other substances, the operation is easy, the reaction is fast, the detection result is reliable, and the copper ion detection kit is suitable for local laboratories.

The invention relates to a method for rapidly detecting urine sugar and uric acid in a solution. According to a method, a printer is adopted, paper AKD is used as a hydrophobic material, a paper chip is made on a piece of filter paper to serve as a detection carrier. Glucose oxidase and horse radish peroxidase are used as catalysis reagents for urine sugar detection, and potassium iodide is used as a coloration reagent. The uric acid detection is performed by adopting a UOD-POD two-enzyme method. The reagents are supported in a detection zone on the paper chip. After the chip is completely dried, standard urine sugar and uric acid solutions or sample solutions different in concentration are detected, photographing is performed, pictures are processed to obtain RGB color intensity values, and standard curves about the RGB color intensity values and the concentrations of the standard solutions are established. The method utilizes high selectivity of enzymatic reaction, combines the simple and convenient paper chip and the visual properties and high sensitivity of multielement detection and chemiluminescence imaging, fully plays the advantages of the three parts, and has the advantages of being low in cost, simple in operation, rapid, easy to operate and the like.

The invention discloses a kit for quantitatively testing free triiodothyronine, which comprises 3,3'-L-Diiodothyronine-gelatin enveloped magnetic bead suspension, a free triiodothyronine series calibrator, a horse radish peroxidase labeled free triiodothyronine antibody, chemiluminescent substrate A solution, chemiluminescent substrate B solution, and PBS buffer solution. The invention also discloses a method for preparing the kit. The kit has the advantages that: the chemiluminescence technology is combined with the immunomagnetic bead technology, the immunomagnetic beads are taken as a reaction carrier, the effective envelop amount of antigen is increased, raw materials are saved and the detection sensitivity and detection speed are obviously improved. A method for enveloping antigen analogs by the labeled antibody is adopted, so the analogs can be specifically combined with the antibody of hormone, but the combination capacity with thyroxine-binding protein is greatly reduced, and the influence of the binding protein on a measurement system is reduced to a large extent.

The invention discloses a preparation method for a sensor based on hydrophilic up-conversion nano-particles and application of the sensor to detection of hydrogen peroxide and glucose. Monodisperse up-conversion nano-particles which are good in water solubility, stable and uniform in size are prepared under an amphiphilic system by adopting an ultrasonic micro emulsion process, utilizing hydrophobic-hydrophobic effects between oleylamine-modified polysuccinimide high polymers and oleic acid on the surfaces of the up-conversion nano-particles and hydrolyzing part of chain elements of the polysuccinimide high polymers under an alkaline condition to form a hydrophilic group; the up-conversion nano-particles are mixed with tetramethyl benzidine and horse radish peroxidase to obtain the hydrogen peroxide and glucose sensor. Compared with a conventional detecting method, the method is small in background interference, strong in signal and low in cost, and has the characteristics of being quick, accurate, high in flexibility and high in selectivity. The hydrogen peroxide and glucose sensor can be used for providing a novel opportunity for monitoring hydrogen peroxide and glucose in real time in a living microenvironment in future.

The invention discloses a preparing method and application of a dual-response sandwich-type immunosensor based on TiO2mesomorphic nanomerter material. The invention is characterized in that a novel dual-response sandwich-type immunosensor is prepared by combining electrochemistry and an electrochemical luminance method on the basis of two kinds of TiO2 mesomorphic nanomerter materials, and the dual-response sandwich-type immunosensor is applicable to detection of interleukin-6. Anatase type TiO2 mesocages materials and ionic liquid are used for fixing Ru(bpy)<3>2+ and an IL-6 antibody respectively, and the Ru(bpy)<3>2+ and the IL-6 antibody are used as a signal probe and a molecular recognition probe; octahedron anatase type TiO2 mesocages materials are used for fixing IL-6 second antibody and acid phosphatase which are marked by horse radish peroxidase, and the IL-6 second antibody and acid phosphatase are subjected to a typical sandwich-type immunoreaction to conduct self-assembly on surface of the electrode to prepare the IL-6 sandwich-type immunosensor. The prepared sandwich-type immunosensor can produce electrochemistry signals and electrochemical luminance signals, wherein signal values and IL-6 concentration present linear states within a range of 10<-6>-90 pg/ml and a range of 10<-8>-90 pg/ml respectively.

The invention provides a preparation method of a determination kit for triazophos chemoluminescence immunoassay and relates to determination kits for triazophos chemoluminescence immunoassay and a preparation method and a using method thereof. The invention solves the technical problem of high minimum detection limit in the conventional direct competitive enzyme-linked immunosorbent assay method. The determination kit for triazophos chemoluminescence immunoassay consists of a triazophos standard substance, a carrier coating a triazophos monoclonal antibody, horse radish peroxidase-labeled triazophos hapten, a chemiluminescent sensitizing solution and phosphate buffer saline (PBS). The kit is prepared by putting the prepared triazophos standard substance, the carrier coating the triazophos monoclonal antibody, the chemiluminescent sensitizing solution and the PBS buffer solution into a box to obtain the determination kit. When the kit is used, the triazophosstandard substance and samples are added into carrier reactive pores respectively and washed, and the chemiluminescent sensitizing solution is added, then the luminescent intensity is determined and specification curve is drawn, and finally the concentration of triazophos in the sample is determined. The minimum detection limit of triazophos is 0.06ng/mL, so the invention can be used in the field of foods.