Silk fibroin hydrogel adopting IPN (interpenetrating polymer network) structure and preparation method of silk fibroin hydrogel

A silk fibroin, interpenetrating network technology, applied in medical science, prosthesis, tissue regeneration, etc., can solve the problem of low elastic recovery rate of hydrogel, achieve excellent light transmittance, excellent compressive strength, gel time short effect

Active Publication Date: 2016-10-12
PHARSUN MEDICAL BIOTECHNICS (SHANGHAI) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese invention patent (CN101502670) discloses a preparation method of silk fibroin hydrogel, which uses ultrasonic vibration to form hydrogel in a short time, but the elastic recovery rate of the formed hydrogel is low

Method used

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  • Silk fibroin hydrogel adopting IPN (interpenetrating polymer network) structure and preparation method of silk fibroin hydrogel

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] This embodiment prepares a silk fibroin interpenetrating network hydrogel, and the specific steps are as follows:

[0023] 1. Preparation of silk fibroin solution:

[0024] Weigh 80 g silkworm cocoon shells with a precision balance, configure 4000 milliliters of sodium bicarbonate sodium carbonate aqueous solution with a mass fraction of 0.01MpH=9.5, (add 4000 milliliters of deionized water to stainless steel, 1 gram of sodium carbonate, 3 grams of sodium bicarbonate ), heated to boiling with an induction cooker, then added cocoon shells, adjusted the power of the induction cooker to the lowest level, continued to boil slightly for 30 minutes, took out the silk and cleaned it with deionized water. Repeat the above test three times to remove sericin, then take it out and place it in an oven at 60°C to dry to obtain pure silk fiber. Dissolve pure silk fiber in lithium bromide solution with a concentration of 9.3 mol / L at a bath ratio of 20:150, dissolve in a constant tem...

Embodiment 2

[0031]1. Preparation of silk fibroin solution: weigh 80 g silkworm cocoon shells with a precision balance, configure 4000 milliliters of sodium bicarbonate and sodium carbonate aqueous solution with a mass fraction of 0.01MpH=9.5, (add 4000 milliliters of deionized water and sodium carbonate to stainless steel 1 g, sodium bicarbonate 3 g), heated to boiling with an induction cooker, then added cocoon shells, adjusted the power of the induction cooker to the lowest level, continued to boil slightly for 30 minutes, took out the silk and cleaned it with deionized water. Repeat the above test three times to remove sericin, then take it out and place it in an oven at 60°C to dry to obtain pure silk fiber. Dissolve pure silk fiber in lithium bromide solution with a concentration of 9.3 mol / L at a bath ratio of 20:150, dissolve in a constant temperature magnetic stirrer at 60±2°C for about 1 h, take it out after cooling, put it into a dialysis bag and seal it, Dialyze in deionized wa...

Embodiment 3

[0036] 1. Preparation of silk fibroin solution: weigh 80 g silkworm cocoon shells with a precision balance, configure 4000 milliliters of sodium bicarbonate and sodium carbonate aqueous solution with a mass fraction of 0.01MpH=9.5, (add 4000 milliliters of deionized water and sodium carbonate to stainless steel 1 g, sodium bicarbonate 3 g), heated to boiling with an induction cooker, then added cocoon shells, adjusted the power of the induction cooker to the lowest level, continued to boil slightly for 30 minutes, took out the silk and cleaned it with deionized water. Repeat the above test three times to remove sericin, then take it out and place it in an oven at 60°C to dry to obtain pure silk fiber. Dissolve pure silk fiber in lithium bromide solution with a concentration of 9.3 mol / L at a bath ratio of 20:150, dissolve in a constant temperature magnetic stirrer at 60±2°C for about 1 h, take it out after cooling, put it into a dialysis bag and seal it, Dialyze in deionized w...

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Abstract

The invention discloses silk fibroin hydrogel adopting an IPN (interpenetrating polymer network) structure and a preparation method of the silk fibroin hydrogel. N-vinylpyrrolidone monomers are subjected to a polymerization reaction under catalysis of horse radish peroxidase to produce polyvinylpyrrolidone, polyvinylpyrrolidone and silk fibroin macromolecules are subjected to molecular chain entanglement, and the IPN hydrogel with a random coil structure as a main structure is formed. According to the solid content of the hydrogel, the content of silk fibroin is 50%-90% by mass, and the content of polyvinylpyrrolidone is 10%-50% by mass. The compression strength reaches 2 kPa or above and the resiliency reaches 90%-100% when the compression ratio of the IPN silk fibroin hydrogel is 60%. The provided silk fibroin hydrogel has the characteristics of high strength, elasticity and transparency and can be used as a polymer material for cornea repairing.

Description

technical field [0001] The present invention relates to a hydrogel material and a preparation method thereof, in particular to a silk fibroin hydrogel with an interpenetrating network and a preparation method thereof. The silk fibroin hydrogel has the characteristics of high strength, elasticity and transparency , can be used as a polymer material for corneal repair. Background technique [0002] Hydrogel is a special soft and wet material with a three-dimensional network structure and a porous interior. It uses water as a dispersion system, has a certain strength and is soft in nature. The porous structure inside the hydrogel is interconnected, which provides a good channel for the transportation of water, nutrients and drugs, and provides an excellent environment for the growth and proliferation of cells. Many tissues in organisms have a hydrogel structure. The gas permeability, ion permeability, and protein deposition of hydrogel materials will directly affect the biomed...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L89/00C08L39/06C08F126/10C08F4/30C08J3/075A61L27/16A61L27/22A61L27/50A61L27/52
CPCA61L27/16A61L27/227A61L27/50A61L27/52A61L2430/16C08F4/30C08F126/10C08J3/075C08L39/06C08L89/00C08L2201/10C08L2205/04
Inventor 卢神州吴峰朱天付华
Owner PHARSUN MEDICAL BIOTECHNICS (SHANGHAI) CO LTD
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