837results about How to "Good biocompatibility" patented technology

The invention encompasses the treatment of urinary incontinence, gastroesophageal reflux disease and the amelioration of skin wrinkles using biocompatible hydrophilic cationic microparticles and a cell adhesion promoter.

The invention discloses a self-crosslinking antibacterial hydrogel material containing carboxymethyl chitosan/oxidized sodium alginate. The material is made from the following components by mass percent: 1-35% of carboxymethyl chitosan, 1-25% of oxidized sodium alginate, 0.01-0.5% of nano-silver and distilled water. The material is prepared by the following steps: preparing the carboxymethyl chitosan by a carboxymethylation reaction of chitosan at the temperature of 10-80 DEG C; preparing the oxidized sodium alginate by an oxidation reaction of sodium alginate at the temperature of 10-45 DEG C; respectively preparing aqueous solution of the carboxymethyl chitosan and aqueous solution of the oxidized sodium alginate; adding the nano-silver solution and blending at certain ratio; and allowing self-crosslinking of the components at the temperature of 10-50 DEG C to produce the antibacterial hydrogel material. The antibacterial hydrogel material has the advantages of low cost, environmental protection, simple process and the like; as the carboxymethyl chitosan has good biocompatibility, the oxidized sodium alginate is nontoxic to human body, and the nano-silver has excellent antibacterial property, the obtained material can be applied to wound dressing, hemostatic materials and the like.

The invention discloses composite hydrogel for adsorption of metal ions and a preparation method thereof. The hydrogel comprises, by weight, 5-95 parts of polyvinyl alcohol, 5-95 parts of chitosan, 0.1-20 parts of modified graphene oxide and 2-6 parts of sodium alginate, the weight ratio between the polyvinyl alcohol and the chitosan is (5: 95)-(95: 5), modified graphite oxide includes modified graphene oxide modified by dopamine and/or modified graphene oxide modified by ferroferric oxide, all components are uniformly mixed in a certain sequence, and the composite hydrogel is obtained under special conditions. The composite hydrogel for adsorption of the heavy metal ions and the preparation method thereof have the advantages that high speed, high efficiency, high adsorption capacity and high selectivity are achieved, the adsorption effect on the heavy metal ions is remarkable, meanwhile, the preparation process is simple, and the operability is high.

The invention belongs to the technical field of high polymer materials, in particular to a thiolated hyaluronic acid conjugate modified by cysteamine, a preparation method and an application thereof. The method comprises the steps of adding 1-ethyl-3-(3-dimethylamino propyl)-carbodiimide (EDAC) and N-hydroxysuccinimide (NHS) in the hyaluronic acid solution to activate the carboxyl of the hyaluronic acid solution, adding cysteamine hydrochloride to make the amino of cysteamine hydrochloride and the carboxyl of hyaluronic acid to form amido link, regulating the proportions of each material and reaction conditions to obtain the thiolated hyaluronic acid conjugates with different sulfhdryl contents. The invention widens the application range of the thiolated reaction. The adhesive properties of the mucosa and the biocompatibility of the conjugate are good. The invention has strong in-situ gel property and supplies good bio-adhesive materials for non-injected preparations, tissue engineering and the like.

The invention belongs to the technical field of pharmaceutical preparations and high molecular materials, in particular to a polymer with tumor organization pH responsiveness, a micelle and a preparation method thereof. In the invention, an amphipathic block polymer is firstly synthesized, and histidine with pH-sensitive groups or a derivate thereof is introduced to the both ends of the amphipathic block polymer. The materials are dissolved in an aqueous solvent in definite proportion to form the micelle by self-assembly; or the materials and medicines are dissolved in an organic solvent together to form a membrane by rotation, and then the membrane is dispersed in water to form a medicine-carrying micelle. The size of the micelle can be controlled by the pH adjustment. The medicine-carrying micelle has different medicine releasing rates for the tumor organization pH and the normal physiologic pH of a human body. The polymer and the micelle thereof are well-behaved carrier materials for the slow release of the medicines.

The invention discloses a hollow core-shell nanometer mesoporous medicament carrying system with magnetism and luminescent performance. In the medicament carrying system, a magnetic Fe3O4 hollow capsule is used as an inner core, mesoporous SiO2 is used as a shell layer, and an organic luminescent material and a biocompatible polymer are modified on the outer surface of the shell layer. A preparation method of the medicament carrying system comprises the following steps of: firstly, preparing monodisperse fusiform beta-FeOOH nanoparticles by a hydro-thermal method, coating mesoporous silicon dioxide on the surfaces of the nanoparticles, removing a pore-forming agent through thermal treatment, and simultaneously, converting the inner core into alpha-Fe2O3 from the beta-FeOOH, so that a cavity is generated in the inner core so as to form a hollow nanometer capsule structure; secondly, converting the inner core into the magnetic Fe3O4 hollow capsule through reduction treatment; and finally, modifying the organic luminescent material and the biocompatible polymer on the surface of the magnetic mesoporous nanometer capsule. The nanometer composite capsule of the invention has the advantages of core-shell structure and cavity structure, uniform particle size, high biocompatibility and excellent magnetism.

The invention provides a preparation method of a calcium silicate/calcium alginate hybrid material with mesoporous silica gel on a surface, which comprises the following steps of: preparing mixed solution of sodium silicate with the mass percentage concentration of 0.5-15 percent and sodium alginate with the mass percentage concentration of 0.1-10 percent, keeping static to remove foam, preparingcalcium chloride solution with the mass percentage concentration of 0.5-10 percent, preparing drops or a film of mixed solution of sodium silicate and sodium alginate via suspension and dispersion orfilm wiping, adding the mixed solution into the calcium chloride solution, cross-linking for 0.5-2h, washing three to five times with deionized water to prepare microspheres or a film material of calcium silicate/calcium alginate hybrid gel, preparing solution with the pH value of 0.1-6.5 using hydrochloric acid, and soaking the prepared calcium silicate/calcium alginate hybrid gel in the prepared solution for 0.5-12h, thereby obtaining the microspheres or the film material of the calcium silicate/calcium alginate hybrid gel with the mesoporous silica gel on the surface.

The invention discloses a high-intensity biological ink material for 3D printing. According to the high-intensity biological ink material for 3D printing, a double-crosslinking network structure is formed by using fibroin for forming a beta folding structure and alginic acid for ionic crosslinking, the thermosensitive quick gelatinizing performance of gelatin is used at the same time, and a good forming effect is achieved. According to the high-intensity biological ink material for 3D printing, the mechanical strength of biological ink can be controlled, and the strength of hydrogel is remarkably improved. Aiming at the application characteristics of different organizations, a series of biological ink with different strengths can be prepared through matching of different materials, the customization design of 3D printing can be achieved for a patient, and the material is more suitable for clinical application.

The invention provides a preparation method of a silk fibroin/metal nanoparticle composite system, belonging to the technical field of nanocomposite materials and preparation methods thereof. The preparation method comprises the steps of fully mixing metal nanoparticles with silk fibroin in a weight ratio of (1:9)-(9:1) so that silk fibroin is non-covalently adsorbed on the surfaces of the metal nanoparticles, thus obtaining the silk fibroin/metal nanoparticle composite system. The metal nanoparticles in the composite system prepared by the preparation method can not aggregate under the conditions that the pH value is 2-11 and the salinity is 0-1000mM, thus improving the stability and biocompatibility of the metal nanoparticles in the physiological environment. The silk fibroin/metal nanoparticle composite system provided by the invention is simple to prepare, is low in cost, has good stability and biocompatibility and has wide application prospects in the biomedical fields of drug sustained release, gene transmission, tissue engineering and the like.

The invention discloses a method for preparing network interpenetrating functional aquagel through the click chemistry and the atom transfer radical polymerization (ATRP) synchronous reaction. The polymer network is generated through the click reaction of monomers A containing three or more than three end alkynyl radicals and monomers B at least containing two end laminated nitrogen radicals, and network interpenetrating polymers are obtained through the atom transfer radical polymerization (ATRP) by double-bond-containing monomers C with functional groups. The click chemistry and the atom transfer radical polymerization are synchronously carried out in the same catalytic system, and the network interpenetrating functional aquagel with the regular structure is obtained. The method of the invention has the advantages of the click chemistry and the ATRP, and also has the advantages of simple operation and fast reaction. The functional aquagel prepared by the method of the invention based on polyethyleneglycol has the characteristics of good biocompatibility, high tensile strength, high water absorption capacity, high swelling ratio, regular structure and the like.

The invention discloses a preparation method of a self-adhering super strong hydrogel, and is characterized in that the preparation method comprises the steps: adding dopamine grafted modified hyaluronic acid into an acrylamide aqueous solution, adding N,N-methylene bisacrylamide and ammonium persulfate, fully stirring evenly, and carrying out sealed vacuumization for 10-15 min; in an ice water bath and under a stirring condition, adding tetramethylethylenediamine, placing at the temperature of 0-4 DEG C, and crosslinking for 12-16 h, to obtain the self-adhering super strong polyacrylamide hydrogel. The prepared self-adhering super strong hydrogel has the following advantages: 1, the adhesion strength is large, is up to 12.3 kPa and is 3-4 times that of a pure polyacrylamide gel; and during use, an auxiliary adhesive or adhesive tape and the like does not need, and the self-adhering super strong hydrogel can adhere onto the surface of any material; and 2, the self-adhering super stronghydrogel has self-healing and self-repairing properties and can be self-healed in 20-30 min.

The invention provides a process for preparing a novel nanometer/ micrometer inorganic- biological degradable polyester composite material comprising, subjecting hydroxyapatite nanometer/micrometer particles and lactic acid to dewatering polycondensation reaction in organic solvent under the condition of heating and stirring, further reacting under toluene azeotropic dewatering condition, the dewatering polycondensation reaction can also be conducted to hydroxyapatite and lactic acid without the presence of solvent, grafting the hydroxyl of the particle surface of the hydroxyapatite to obtain the chemical constitution of lactic acid ester or low polylactic acid ester - hydroxyapatite, under the condition of waterless and oxygen free, using stannous octoate as initiating agent to trigger epsi-caprolactone, lactide, glycolide or cyclic ester monomer, and ring-opening polymerizing to obtain the homopolymer of epsi-caprolactone, lactide or glycolide biodegradable polyester and the nanometer/micrometer composite material of their dyadic and ternary copolymer.

The invention discloses application of nano-selenium in an antineoplastic drug carrier. The nano-selenium is used as a carrier and is used for loading an antineoplastic drug to obtain an antineoplastic drug compound system. The nano-selenium is applied to the antineoplastic drug compound system, so that the prepared antineoplastic drug compound system can bring the synergistic action of an antineoplastic drug and the nano-selenium in resisting tumors into play, and while the penetrability of the antineoplastic drug is enhanced, the toxic or side effect of the antineoplastic drug to human body normal tissues is also reduced. The antineoplastic drug compound system can be preserved in the form of hydrosol in a liquid phase, the preparation process is simple, the condition is mild, the stability is high, and the preservation time is long, so that the antineoplastic drug compound system is suitable for popularization and application.

The invention relates to oxidized graphene modified by hyaluronic acid and a preparation method and an application of a medicinal composition of the oxidized graphene modified by the hyaluronic acid and can effectively solve the problem of non-targeting property of conventional photothermal therapy and chemotherapy technology. For solving the problem, the invention adopts the technical scheme that the hyaluronic acid is in covalent linkage with oxidized graphene by alkylene diamine which is used as a link arm and a nano layer can be formed in a water medium; the hyaluronic acid is low molecular weight hyaluronic acid of which the molecular weight is equal to or less than 400 kD and is equal to or higher than 500 Daltons; and the link arm is alkylene diamine of which the carbon number is 2 to 12. The oxidized graphene modified by the hyaluronic acid has excellent biocompatibility, water solubility and stability and is an innovation on cancer treatment medicaments.

The invention relates to a preparation method of a composite nanofiber tissue repair scaffold which contains collagen. The steps are as follows: firstly, collogen spinning solution is prepared; secondly, thermosplastic polyurethane spinning solution is prepared; thirdly, the collogen spinning solution and the thermosplastic polyurethane spinning solution are mixed according to the proportion and agitated to be even, and then static spinning solution is obtained; fourthly, the static spinning solution is arranged in a static spinning device, the spinning industry parameter is adjusted, the mold shape of a receiving device is changed, and then static spinning composite nanofiber tissue repair scaffolds with different shapes are obtained. The scaffold with the material has good biological compatibility and mechanical property, and higher void factor, and the scaffold is hopeful to be used as the repair scaffold materials of the tissues such as blood vessel, nerve, valve, etc.

The invention relates to a flexible temperature sensor based on a graphene nanometer wall and a preparing method thereof. The temperature sensor comprises polymer and a graphene nanometer wall body. The polymer is combined to the lower surface of the graphene nanometer wall body or embedded in the upper surface of the graphene nanometer wall body. The preparing method comprises the steps that after the graphene nanometer wall body made of the tubular CVD is transferred through the polymer transferring method, a graphene nanometer wall body/polymer double-layer structure is formed, the two ends of the graphene nanometer wall body are connected through a silver paste coating method to serve as an electrode with silver or other metal wires, spin coating can be carried out on upper layer of polymer, and a polymer/graphene nanometer wall/polymer three-layer structure is formed. The temperature sensor made of the graphene nanometer wall/polymer has the advantages of being simple in structure, convenient to machine, high in flexibility, light in weight, good in flexibility and biology compatibility and the like, and has very good application prospects in the fields of instruments, meters, photoelectric devices, flexible electronic skins and the like.

Belonging to the field of biomedical materials, the invention relates to a preparation method of a polysaccharide-nanometer bacterial cellulose composite wound dressing. The method specifically consists of the steps of: taking a nanometer bacterial cellulose membrane for pretreatment and purification treatment so as to obtain purified nanometer bacterial cellulose, then preparing a polysaccharide solution with a concentration of 0.1-10%, removing part of water from the nanometer bacterial cellulose membrane, then compounding it with a polysaccharide solution by means of soaking, tape casting or spraying so as to obtain a polysaccharide-nanometer bacterial cellulose composite membrane, then conducting extrusion dewatering and ionizing radiation disinfection, thus obtaining the polysaccharide-nanometer bacterial cellulose composite wound dressing. The dressing of the invention has the advantages of good biocompatibility, cellular affinity and antibacterial property as well as mechanical property, excellent permeability, breathability or drainage, and moisture absorption effects, and has the efficacy of sterilization, inflammation diminishing, hemostasis, pain relieving, infection reduction and the like. At the same time, the dressing can promote wound surface drying, incrustation and healing, and can be used to treat burns or scalds, open wounds, ulcers, chronic wounds and stubborn infected wound surfaces, etc.

The invention discloses a method for preparing a medical high molecular material by a magnetron sputtering technology. The method comprises the following steps of: fixing or moving a medical high molecular material to or through a treatment bench in a vacuum chamber of magnetron sputtering equipment, pumping vacuum, introducing gas, switching on power supplies of the treatment bench and a target bench, and treating to obtain the medical high molecular material with high surface infection resistance and biological compatibility. The method is simple in operation and does not affect the physical-chemical structures of the material, the treated high molecular material has a stable surface structure and long-acting broad-spectrum infection resistance, and the cell compatibility of the treated high molecular material is not lower than the inherent biological compatibility of the material.

The invention discloses a growth factor-loaded collagen group composite material and a preparation method thereof. The growth factor-loaded collagen group composite material is prepared from collagen, a growth factor, chitosan and sodium heparin, a dense layer collagen film and a loose layer collagen film form a double-layer structure, and a growth factor-loaded chitosan-heparin nano particle is compounded between the double layers. The dosage of the growth factor is 50-10,000ng/cm<2>; and the average thickness of the double-layer film is 1-4mm. The preparation method comprises the steps of preparing the dense collagen film and the loose collagen film, preparing the growth factor-loaded nano particle, compounding the collagen films and the nano particle, and the like. The collagen group composite material prepared by the invention has orient difference control slow release function on the loaded growth factor, has a slow release period longer than 20 days, maintains the activity of the growth factor and can be used for the restoration treatment of the disease defect tissue.

The invention relates to a pH and oxidation-reduction dual-sensitive layer cross-linking nanoparticle as well as a preparation method and an application thereof. A hydrophilic layer which at least contains PCB (Polycarboxylate Betaine) or PEG (Polyethylene Glycol) is positioned on the surface of the nanoparticle, a hydrophobic nuclei which at least contains a pH-sensitive polymer unit is positioned in the nanoparticle, and an S-S (Disulfide Bond) cross-linking layer which is formed through cross-linking reaction between PDS (Polymethacrylamide Ehtylpyridine Disulfide) units is positioned between the hydrophilic layer and the hydrophobic nuclei. A tumor targeted group can be modified on the hydrophilic layer positioned on the surface of the pH and oxidation-reduction dual-sensitive layer cross-linking nanoparticle, and the gathering of the nanoparticle on a tumor tissue and the endocytosis of the nanoparticle on the tumor cell are accelerated through the specific targeted effect of the nanoparticle on a tumor cell. The pH and oxidation-reduction dual-sensitive layer cross-linking nanoparticle disclosed by the invention has the advantages of good biocompatibility and low toxicity. According to the pH and oxidation-reduction dual-sensitive layer cross-linking nanoparticle, a cross-linked structure is dissociated under the action of glutathione inside the tumor cell, so that the release of a medicine is promoted. The preparation method disclosed by the invention is simple, convenient, good in stability and conveniently operated and popularized.

The invention discloses a collagen/chitosan micro-nano fiber composite hemostatic membrane material and a preparation method thereof. The preparation method is characterized by comprising the following steps of: extracting a medical biological skin sheet serving as a raw material by an ultrasonic technology to prepare I type collagen; mixing hexafluoroisopropanol and acetic acid according to different volume ratios to prepare a spinning solvent; mixing the prepared I type collagen and chitosan in a certain mass ratio and adding the mixture into the spinning solvent; stirring in an ultrasonic cleaner at room temperature until the material is transparent to prepare electrostatic spinning mother liquid at the concentration of 4 to 10 percent; injecting the electrostatic spinning mother liquid into an electrostatic spinning machine and performing electrostatic spinning to obtain a collagen/chitosan micro-nano fiber composite membrane; soaking the collagen/chitosan micro-nano fiber composite membrane into natural biomacromolecules and Chinese herbal medicines sequentially; and freeze-drying to obtain the collagen/chitosan micro-nano fiber composite hemostatic membrane material. The collagen/chitosan micro-nano fiber composite hemostatic membrane material has excellent biocompatibility, biodegradability, adhesion property and the like, can quickly stop bleeding, resist inflammation, ease pain and promote wound healing, and can be applied to trauma hemostasis and repair and general civil hemostasis emergency treatment.

A staltic soluble cellulose sponge is proportionally prepared from carboxymethyl cellulose sodium and chitosan through dissolving carboxymethyl cellulose sodium in distilled water, dissolving chitosan in diluted acetic acid, mixing, adding polyethanediol 400, cane sugar and glycerin, stirring, laying aside while defoaming, filming, and vacuum freeze-drying.

The invention provides devices, compositions and methods for maintaining conditions in a cell culture and for measurement of conditions in the cell culture. In particular, the invention provides hydrogel materials, apparatus and methods for several non-invasive techniques of maintaining glucose and pH levels in cell cultures at near-optimal levels and the non-invasive measurement of pH levels in cell cultures.

The invention discloses a preparation method of biological active glass/chitosan composite porous support material, which adopts a perfusion method to compound chitosan and sol-gel biological glass porous support material prepared by a foam impregnation method. The invention comprises the steps of preparing pure biological active glass support material through the foam impregnation method, preparing chitosan solution and compounding biological active glass/chitosan composite support through the perfusion method, wherein the preparation of pure biological active glass support material through the foam impregnation method comprises preparation of size of the biological active glass, selection, impregnation and desiccation of polyurethane foam template.. During the preparation process of theinvention, shape, porosity and distribution of aperture are easy to be regulated and controlled. The prepared support material is provided with three-dimensional reticulate backbone structure, and themechanical intensity, biocompatibility and bioactivity thereof are excellent and degradable; the pressure strength thereof can achieve 1.15-1.30 MPa; apparent porosity can achieve 70%-80%. The support material is capable of meeting the requirements of providing support for defective parts of tissue. The invention has simple and practical method, thus being easy for large-scale production.

A bone tissue regeneration guiding membrane. The invention employs an antigen-extracted mammal tissue membrane, which has a double layer structure of a compact layer and a loosening layer. The compact layer is formed by tightly arranged collagen fibers, and an external side of the compact layer is composited with a collagen coating containing active polypeptides; the loosening layer is formed by interlaced collagen fibers and is composited with ossification active factors. The prepared guiding membrane can prevent soft tissue from evolving and promote new bone formation, has good biological compatibility, hydrophilism and bone induction capability, and can be completely degraded in body without generation of harmful substances. Besides, the guiding membrane has an effect of isolating cell evolving, a characteristic of slow degradation and a longer retaining time in body than a current guiding membrane prepared from natural high-molecular polymer, and can provide enough time and space for new bone growth. The method of the invention is simple, and raw materials have wide sources and low prices, so that the method is suitable for large-scale industrial production and beneficial to lowering medical costs and mitigating patient burdens.

The present invention discloses the preparation process of microcapsule with included anticancer medicine. The process adopts colloid particle containing polyelectrolyte as template, assembles polyelectrolytes with dislike charges via layer-by-layer self-assembling to the surface of colloid particle, and dissolves or decomposes the colloid particle to obtain hollow polymer microcapsule containing polyelectrolyte. The microcapsule has wall micro structure and thickness capable of being regulated precisely in nanometer size, and the contained polyelectrolyte can interact with the medicine so as to include the medicine into the microcapsule. The included medicine has controllable release speed. Introducing polyelectrolyte with grafted polyglycol to the surface of the microcapsule can raise the biocompatibility of the microcapsule. The process of the present invention is simple, feasible and repeatable, and suitable for inclusion and controllable release of various kinds of water soluble anticancer medicine.

The invention discloses a biomedical adhesive for crosslinking reaction with wound skin tissues and a preparation method thereof. The biomedical adhesive is prepared by a method with following steps of firstly, using sodium periodate and oxidized sodium alginate to prepare dialdehyde sodium alginate, dialyzing, drying, and dissolving a product into an MES (methyl ester sulfonate) buffer solution; then, adding carboxymethyl chitosan, stirring and reacting for 6-12h, sequentially adding a certain amount of N-hydroxy succinimide, 1-(3-dimethylamino propyl)-3-ethylcarbodiimide hydrochloride and dopamine, continuing to stir and react for 8-24h under the nitrogen protection, dialyzing, freeze-drying, and storing in the shade and dry area. Before use, a wound is pretreated by hydrogen peroxide, the freeze-drying adhesive is dissolved by a PBS (poly butylenes succinate) buffer solution, and is immediately coated to the wound, and the wound can be adhered within 2-10min. The biomedical adhesive has the advantages that the adhering strength in the in-vitro wetting environment is stronger, the biocompatibility is good, the biodegradation is realized, and the curing of wounds is effectively promoted.

The invention belongs to the field of biomedical materials, and relates to a biological functionalized nano titanium material. The surface of the titanium material is provided with a TiO2 nano tube array with tube diameter of 30-100nm, and the surface of the TiO2 nano tube array is also fixed with a bone morphogenetic protein 2(BMP2). A preparation method of the biological functionalized nano titanium material comprises the steps of: preparing the TiO2 nano tube array with the tube diameter of 30-100nm on the surface of the titanium material by adopting an anode oxidizing method, and fixing the BMP2 on the surface of the TiO2 nano tube array through polydopamine to obtain the biological functionalized nano titanium material. The biological functionalized nano titanium material has better biocompatibility; on one hand, the TiO2 nano tube array with proper tube diameter can provide a proper topological structure for the cell growth, on the other hand, the BMP2 is used as a growth factor capable of promoting the cell differentiation, and the proliferation and differentiation capacity of the cells on the surface of the titanium material can be improved through the synergistic effect of the topological structure and the chemical components, thus the osseointegration of the titanium material is improved.

The invention discloses a preparation method of an injectable hyaluronic acid hydrogel. The method comprises the following steps: initiating a condensation reaction of an amino group of adipic dihydrazide and a carboxyl group of the main chain of HA (hyaluronic acid) by 1-ethyl-3-(3-dimethylamino)carbodiimide hydrochloride to obtain a hyaluronic acid-adipic dihydrazide (HA-ADH) derivative with the main chain possessing the amino group, and reacting N-acryloxysuccinimide with the amino group of the HA-ADH to obtain an acrylate functionalized hyaluronic acid derivative; and cross-linking the acrylate functionalized hyaluronic acid derivative with a bismercapto cross-linking agent to form the injectable hyaluronic acid hydrogel. The above acrylate functionalized HA-AC synthesis method is simple and easy, and is suitable for industrial amplified production; the environmental protection, biological inertness and reaction speed controllability of a Michael addition reaction lay a good foundation for the application of the Michael addition reaction in the injectable hyaluronic acid hydrogel; and the HA-AC chemically cross-linked hydrogel has better stability and mechanical performances than physically associated injectable gels.

Prosthetic ligaments and tendons comprising ligament- or tendon-mimicking nanofibers and methods of making such nanofibers and prosthetic ligaments and tendons.