176 results about "Superoxide" patented technology

The invention provides lactobacillus plantarum XZFMCC101.23155, a probiotic agent and application of the lactobacillus plantarum XZFMCC101.23155 and belongs to the technical field of microorganisms. The invention provides lactobacillus plantarum XZFMCC101.23155, and the preservation number of the lactobacillus plantarum XZFMCC101.23155 is GDMCC No: 65783. The lactobacillus plantarum XZFMCC101.23155 can tolerate hydrogen peroxide, a fermentation product of the lactobacillus plantarum XZFMCC101.23155 has the capacity of removing DPPH, hydroxyl free radicals and superoxide anions, and the total antioxidant activity is high. The fermentation product can also significantly reduce the MDA content in mouse serum, significantly improve the SOD level in mouse serum, improve the GSH content in mouse serum and the like. The lactobacillus plantarum XZFMCC101.23155 and commercial zymophyte are jointly used as a leavening agent to prepare the cheese, and the antioxidant activity of the cheese can be remarkably improved.

The invention belongs to the technical field of environmental engineering, and particularly relates to a method for enhancing the reaction efficiency of a Fenton-like fluidized bed through intelligent magnetic control. Specifically, customized composite magnetic particles are placed in a columnar Fenton-like fluidized bed reactor with an electromagnetic coil array distributed on the periphery, percarbonate activated by iron and aluminum phosphate is used as an oxidizing agent, and magnetic fields with different directions and intensities are applied to the magnetic particles by utilizing the electromagnetic coil array; the magnetic particles guide the fluid to form a required flowing form, and the fluid flowing path and the mixing process are highly accurately controlled, so that the aims of fully mixing the composite magnetic particles with the percarbonate ions, efficiently generating secondary oxidants such as hydroxyl free radicals, carbonate free radicals and superoxide anion free radicals and efficiently degrading pollutants are fulfilled.

The invention relates to the field of oral drinks, in particular to a composition and drink for improving eye health based on leavening and a preparation method of the composition and drink. 3.2 to 5.5 parts of a schizophyllum commune fermentation product; 2.1 to 4.5 parts of yeast powder containing SOD; 1.5 to 3.0 parts of sea rice leavening; 3.0 to 5.0 parts of mulberry fruit fermentation liquid; and 1.2 to 2.5 parts of a tomato extract. The yeast powder containing SOD can specifically remove superoxide anion free radicals, the mulberry fermentation liquor is rich in polyphenol, and the tomato extract has a relatively strong anti-oxidation effect and can achieve a synergistic effect, so that the ABTS free radical scavenging capacity and the microcirculation effect are remarkably improved in multiple dimensions; the sea rice leavening is rich in various trace elements and can effectively relieve asthenopia; schizophyllan contained in the schizophyllum commune leavening has a macromolecular polysaccharide spiral result, forms a gel-like substance in an aqueous solution, and can uniformly disperse and wrap each active ingredient, so that the slow-release effect of the active ingredients is improved.

The invention provides a coordination nano assembly of chiral cyclopeptide and manganese ions as well as a preparation method and application of the coordination nano assembly. According to the method, a manganese superoxide dismutase protein structural domain and a unique tumor microenvironment of melanoma high tyrosinase are combined, and polypeptide sequences L-YD-hL-DD-h, L-YL-HL-DL-H and D-yD-hD-dD-h with different chirality are designed for the first time; the chirality of the polypeptide is regulated and controlled, the in-vivo circulation stability and the cell entry efficiency of the nano assembly are improved, the coordination self-assembly of manganese and different chiral cyclopeptides is realized, and the in-situ oxidation of an in-vivo tumor microenvironment is simulated for photo-thermal therapy and cell membrane coating. The method disclosed by the invention is simple, mild in experimental condition and easy to operate, the prepared cell membrane coated nano assembly not only has long circulation stability and high cell entry efficiency, but also can realize mild photo-thermal therapy through in-situ oxidation, releases manganese ions to activate a CGAS-STING pathway for immunotherapy of tumors, and has a wide application prospect. The potential application value is realized in the field of combined treatment of tumors.

A lithium oxygen electrochemical cell includes an anode that includes lithium metal, a porous oxygen cathode that includes a conductive carbon and aluminum, and an electrolyte.

The invention provides an aza-BODIPY molecule with NIR-II fluorescence emission and I-type photodynamic activity and a preparation method thereof. The aza-BODIPY molecule has good light absorption in a near-infrared (NIR) region, the emission peak of the aza-BODIPY molecule is prolonged to a near-infrared second region (NIR-II), and the aza-BODIPY molecule shows an ultrahigh NIR-II fluorescence quantum yield of 13%. Meanwhile, the photosensitizer prepared by adopting aza-BODIPY molecules can effectively generate I-type active oxygen species, namely superoxide anion free radicals, under the excitation of 808nm laser. On the basis, high-resolution tumor fluorescence imaging can be realized by utilizing NIR-II fluorescence of the aza-fluorine boron fluorescent sensitizer, and the aza-fluorine boron fluorescent sensitizer is used for accurate and efficient NIR-II fluorescence imaging mediated I-type photodynamic therapy of deep tumor tissues.

The invention belongs to the technical field of organic dye synthesis and application, and discloses a conjugated compound containing a benzothiophenazine structure as well as a preparation method and application thereof, and the conjugated compound containing the benzothiophenazine structure has excellent photophysical properties, singlet oxygen yield and superoxide anion generation capacity. The novel conjugated compounds ENBO-NI and ENBS-NI containing benzothiophenazine structures are successfully prepared by replacing a benzene ring of Nile blue with a cycloalkane structure and adjusting the electron donating ability of an R1 group. The two compounds have near infrared fluorescence emission, good solubility and relatively high ROS yield. Compared with a classical photosensitizer NB, the singlet oxygen yield of ENBO-NI is increased by about 117.65%, and the singlet oxygen yield of ENBS-NI is as high as 70%. The absorption wavelength of the conjugated compound is in a red light wave band. The preparation process is simple and efficient, large-scale production is easy, and the preparation method has a wide clinical application prospect.

The invention discloses a superoxide photocatalytic oxidation waste vegetable wastewater treatment device and method, and relates to the technical field of sewage treatment.The superoxide photocatalytic oxidation waste vegetable wastewater treatment method comprises the following steps that S1, wastewater is input into an ME micro-electrolysis reactor, acid is added for a stirring reaction, then intermittent aeration is conducted, and wastewater is obtained; the wastewater is in contact with filler in the ME micro-electrolysis reactor under the action of airflow to generate micro-electrolysis reaction; and S2, inputting the wastewater subjected to the micro-electrolysis reaction into a sedimentation tank, and adding alkali for stirring reaction. According to the invention, decolorization, chain scission, ring scission and molecular structure destroy are carried out through micro-electrolysis, difficult degradation is changed into easy degradation, oxidation decolorization and peculiar smell removal are carried out through superoxide photocatalytic oxidation, macromolecules are oxidized into micromolecular substances, and the micromolecular substances, nitrogen, phosphorus and the like are finally converted into carbon dioxide, water, nitrogen and other inorganic substances by the ecological composite bed. The method plays an important role in developing the vegetable industry, solving the problem of environmental pollution caused by industrial development and realizing cyclic utilization of fresh water resources.

The invention provides a trimetal nano enzyme as well as a preparation method and application thereof. The ABC trimetal nano-enzyme disclosed by the invention has multi-enzyme activities of glucose oxidase, peroxidase, catalase, glutathione peroxidase, nicotinamide adenine dinucleotide phosphate oxidase and the like, and can be used for enhancing the catalytic treatment on tumors. In a tumor microenvironment, the component A has glucose oxidase-like activity, and can catalyze endogenous glucose to generate hydrogen peroxide to enhance the generation of hydroxyl radicals; the component B has peroxidase-like and glutathione-like peroxidase activity, and can generate hydroxyl radicals and consume glutathione; the component C has peroxidase-like activity and catalase-like activity and can generate hydroxyl radicals, and generated oxygen can improve the glucose oxidase-like activity of the component A. In addition, the component BC has the activity of nicotinamide adenine dinucleotide phosphate oxidase, and oxygen can be catalyzed to generate superoxide anions by utilizing the excellent electron transmission characteristic of the interface of the component BC.

The invention discloses a lactobacillus plantarum YM-7, the preservation number of the lactobacillus plantarum YM-7 in Guangdong Microbial Culture Collection Center is GDMCC No: 65465, and the lactobacillus plantarum YM-7 obtained from fermented food is high in safety, has an inhibition effect on staphylococcus aureus, escherichia coli and candida albicans, and can be used for preparing a food additive. The strain TN-7 has the effect of removing DPPH, hydroxyl free radicals and superoxide anion free radicals, so that the strain TN-7 can be used as a food additive, and a new way is provided for development of antioxidant and antibacterial food additives.

The invention relates to the technical field of natural product extraction, in particular to a licorice herb residue extract and an extraction method.The extraction method comprises the steps that firstly, choline chloride and glycerinum are mixed, heated and stirred, then pure water is added into a DES solvent, and a DES solution is obtained; then adding the licorice herb residue powder into a DES solution, and carrying out ultrasonic extraction at room temperature to obtain a primary extracting solution; centrifuging and precipitating the primary extracting solution, and freeze-drying the supernate to obtain the liquorice herb residue extract. Compared with a traditional ethanol reflux extraction method, the method disclosed by the invention has the advantages that the extraction of the active substances in the licorice herb residues is remarkably improved, and the clearance rates of the licorice herb residue extract prepared by the method to DPPH, superoxide anions and superoxide anions are 93.02%, 80.95% and 73.22% respectively, and are improved by 17.08%, 15.73% and 21.08% respectively compared with the ethanol reflux extraction method; the biological activity and the antioxidant activity are excellent.

The invention discloses a banana drought-enduring functional gene MaPUB21 and application of the banana drought-enduring functional gene MaPUB21 in improvement of plant drought-enduring capability. A nucleotide sequence of the gene is shown as SEQ ID NO.2. The invention further discloses a method for preparing the banana drought-enduring functional gene MaPUB21. According to the invention, a silence vector (MaPUB21-pTRV) aiming at the MaPUB21 gene is constructed, and an agrobacterium-mediated method is utilized to carry out instantaneous silence in the Musa communis. Drought tolerance analysis shows that after drought stress treatment, compared with a wild type, the content of malonaldehyde, superoxide anions and hydrogen peroxide of the MaPUB21 silent strain is remarkably reduced, and the content of proline and the activity of peroxidase are remarkably improved. The result shows that the drought tolerance of the plant can be effectively improved by silencing the MaPUB21 gene. The gene provided by the invention provides a new resource for plant stress-resistant molecular breeding, and is of great significance for cultivating drought-resistant plants and improving the utilization rate of drought land.

The invention belongs to the technical field of traditional Chinese medicine extraction, and particularly relates to a fructus forsythiae extract and a preparation method thereof.The method comprises the following steps that 1, pretreatment is conducted, specifically, fructus forsythiae is added into water to be braised, then dried and ground, and fructus forsythiae powder is obtained; (2) adding the fructus forsythiae powder into a deep-eutectic solvent, then adding an enzyme preparation, carrying out enzymolysis extraction under an ultrasonic condition, and after the enzymolysis extraction is finished, filtering out precipitates to obtain a fructus forsythiae extract; wherein the enzyme preparation comprises cellulase, pectinase and saccharifying enzyme; the eutectic solvent is prepared from choline chloride, glycerol and polyethylene glycol in a molar ratio of 1: 1: (2-3). The fructus forsythiae extract obtained by the method has a very high elimination rate on DPPH free radicals, superoxide anion free radicals and ABTS free radicals, and can be applied to antioxidant products as a raw material.

The invention provides a grain boundary defect-enriched cerium dioxide nano catalyst and a preparation method thereof, and the preparation method comprises the following steps: preparing a cerium salt solution with nitrate radicals, reacting to obtain cerium formate and basic cerium carbonate precursor products, and washing, drying and roasting to obtain the grain boundary defect-enriched cerium dioxide nano catalyst. The cerium dioxide nano-catalyst prepared by the method can form an oxygen vacancy defect, promote the adsorption and activation of O2, form peroxy, superoxide and other active oxygen species, and improve the catalytic oxidation activity. According to the method, the covalence of Ce-O bonds is effectively reduced by destroying the periodicity of CeO2 crystal lattices. The reduction of covalence can weaken the constraint strength that surface lattice oxygen atoms are surrounded by Ce atoms and enhance the release reactivity of the surface lattice oxygen atoms essentially, so that the formation of oxygen deficiency is further promoted, the CeO2 adsorption is enhanced, the activity of activating O2 to form active oxygen for oxidation reaction is enhanced, and the oxidation reaction efficiency is improved. Furthermore, the catalyst has good catalytic performance on toxic and harmful substances such as chlorobenzene, sarin toxic agent simulation agents, CO and the like.

The invention belongs to the technical field of fermentation and metagen preparations, and particularly relates to application of bifidobacterium breve metagen in the aspects of scavenging free radicals and relieving oxidative aging. According to the present invention, the oxidative free radical scavenging experiment results show that the bifidobacterium breve NKU BB 1-2 metagen has good oxidative free radical scavenging ability, and has good scavenging ability on hydroxyl free radicals, superoxide anions and DPPH free radicals; meanwhile, through component analysis, it is found that the bifidobacterium breve NKU BB 1-2 metagen contains various antioxidant and anti-aging bioactive substances such as flavonoids, indoles, amino acids, bile acids and ceramides; a cell model experiment verifies that the bifidobacterium breve NKU BB 1-2 metagen can obviously relieve oxidative damage to the cells and improve the oxidation resistance of the cells, so that oxidative aging is relieved.

The invention provides a new strain XP1 of Leuconostoc lactis, and the preservation number of the new strain XP1 of the Leuconostoc lactis is CGMCC (China General Microbiological Culture Collection Center) No.31789. The invention also provides a preparation method of the new strain XP1 of the Leuconostoc lactis. The novel leuconostoc lactis strain XP1 provided by the invention has relatively good pullulanase production activity and acid production characteristic; in a hemolysis experiment, no hemolysis phenomenon is generated, hemolysis is avoided, and safety and non-toxicity are achieved; the compound has drug resistance to rifampicin and has an inhibition effect on pathogenic bacteria such as staphylococcus aureus and escherichia coli; the strain XP1 has good oxidation resistance, the DPPH free radical scavenging rate is 85.20%, the superoxide anion free radical scavenging rate is 63.50%, the ABTS < + > free radical scavenging rate is 55.12%, and the strain XP1 has good application prospects in food development, health care product preparation and medicine preparation.

The invention relates to an application of a phosphorus vacancy mediated transition metal-based cathode material in electro-Fenton-like selective degradation of metronidazole antibiotics. The phosphorus vacancy-mediated transition metal-based cathode material is NiCoP-Vp, foamed nickel is used as a substrate, and a phosphorus vacancy-containing NiCoP catalyst is loaded on the surface of the substrate; the NiCoP catalyst containing the phosphorus vacancy is in a nanoneedle shape, and a large number of holes are formed in the surface of the NiCoP catalyst. The transition metal-based catalyst containing the phosphorus vacancy has higher electron transmission efficiency and relatively larger electrochemical active area, more active sites can be exposed, and 100% removal of metronidazole can be selectively realized. The fully exposed active sites provide more sites for the adsorption and activation of H2O2 and O2, the improved charge transfer can accelerate the redox cycle of transition metals, the two can cooperatively regulate and control to generate superoxide free radicals O2 <.-> and high-valence metal oxygen, and finally the selective efficient degradation of metronidazole antibiotics is realized.

The invention provides a method for delaying post-harvest physiological deterioration of cassava and reducing superoxide anion level of cassava through nickel sulfate treatment, and belongs to the technical field of post-harvest preservation of cassava. According to the method, cassava is treated through nickel sulfate contact, the superoxide anion level of cassava is reduced, and post-harvest physiological deterioration of cassava is delayed; therefore, physiological browning and deterioration of the cassava after picking are effectively prevented, and good storage quality of the cassava can be maintained. The method is high in practicability and simple and convenient to operate, can effectively prolong the storage time of the cassava, and has good development and application values in the links of storage, transportation, industrial production and the like of the cassava as an industrial raw material.

The invention relates to the technical field of nano-materials, and discloses a heterogeneous diatomic nano-enzyme as well as a preparation method and application thereof. The invention provides a space confinement regulation and control strategy which is used for constructing a heterodiatomic catalytic center on a nitrogen-doped carbon skeleton and preparing nano-enzyme with structural stability and synergistic catalytic performance. According to the method, a confinement framework formed by self-assembly of first metal ions such as cobalt ions and second metal ions such as zinc ions and organic ligands is used as a template, third metal ions such as iron ions are introduced, and then carbonization heat treatment is performed in a protective gas atmosphere, so that bimetal sites with stable bridging configuration are formed in situ. The obtained nano-enzyme has the catalytic activity of various antioxidant enzymes, including catalase and remarkable superoxide dismutase activity, and has more excellent free radical scavenging capacity compared with a single-metal or non-bridging system.

The invention discloses a photochromic TiO2-PEG200 (Polyethylene Glycol 200) nano particle and an application of the photochromic TiO2-PEG200 nano particle in photocatalytic oxidation of a C-H bond. The catalyst is synthesized through a solvothermal method, PEG200 is used as a solvent and a modifier, and self-doped Ti < 3 + > and surface oxygen vacancies are introduced into TiO2. Under the irradiation of blue light (405nm), the catalyst generates a photochromic effect, Ti < 4 + > is reduced into high-concentration Ti < 3 + > in situ, and meanwhile, a large amount of superoxide free radicals (O < 2 + >) are generated. A photo-generated hole directly activates an ethylbenzene alpha-C-H bond to generate a benzyl free radical, and the benzyl free radical reacts with a superoxide free radical (O2.) and is finally converted into acetophenone. The catalyst has excellent visible light response and charge separation efficiency, and the acetophenone yield is up to 56 mmol.g <-1 >. H <-1 > and is 10 times that of traditional TiO2. The reversible circulation of Ti < 3 + > / Ti < 4 + > enables the catalyst to maintain good stability, and the activity is still maintained at 95% or above after five times of circulation. The operation conditions are mild (room temperature and normal pressure), a strong oxidant is not needed, an efficient, stable and environment-friendly photocatalysis solution is provided for C-H bond activation, and the method has important application value in the field of fine chemical synthesis.

This invention relates to a vehicle air conditioner, and the object of this invention is to improve the assemblability, maintenance, and repairability of a photocatalyst module by improving the intake housing structure, and to reduce air vortices generated at the air intake port side during air intake by the blower and the rumbling noise caused by these air vortices. To achieve this object, the vehicle air conditioner of this invention includes: an intake section that draws in internal and external air to blow them into the interior space of the vehicle; and a photocatalyst module that releases superoxide radicals into the air blown from the intake section into the interior space of the vehicle to purify the air, wherein the photocatalyst module is disposed on the outer surface portion of the downstream side of the blower housing of the intake section, and interference-avoidance recesses are formed on the outer surface portion of the intake housing corresponding to the periphery of the photocatalyst module disposed in the blower housing.

The invention belongs to the field of superoxide anion free radical detection, and particularly relates to a reversible fluorescent probe for detecting superoxide anion free radicals as well as a synthesis method and application of the reversible fluorescent probe. The fluorescent reversible probe is 3-(benzothiazole-2-yl)-[1, 1 '-biphenyl]-2, 3', 4 '-triphenol. The preparation method comprises the following steps: dissolving 3-bromo-2-hydroxybenzaldehyde in absolute ethyl alcohol, adding sodium hydrogen sulfite and o-aminothiophenol, and carrying out heating reflux reaction; dissolving a reaction product and 3, 4-dimethoxyphenylboronic acid in tetrahydrofuran, adding a potassium carbonate solution and tetrakis (triphenylphosphine) palladium, and carrying out heating reflux reaction; and dissolving the product obtained by the reaction in dichloromethane to completely dissolve the solid, adding boron tribromide, and reacting under the ice salt bath condition to obtain the target probe. The fluorescent reversible probe can be used for superoxide anion free radical detection, and the detection method is simple, convenient to operate, good in selectivity, high in sensitivity, long in emission wavelength, large in Stokes shift and high in signal-to-noise ratio.

The invention belongs to the technical field of biological medicines, and discloses a carbazole / benzo [cd] indole compound as well as a preparation method and application thereof. The carbazole / benzo [cd] indole compound has a structure as shown in a general formula I: # imgabs0 #, wherein R is one of NO2, NH2, Br and N (Ph) 2. The carbazole / benzo [cd] indole compound can generate active oxygen molecules after illumination, including superoxide anions and hydroxyl radicals, and can be applied to preparation of drugs for photodynamic therapy.

This invention discloses the application of a porcine-derived Bacillus in antioxidant activity, wherein the porcine-derived Bacillus is used to prepare antioxidant products. The porcine-derived Bacillus is Bacillus subtilis YB-X-721, classified as Bacillus subtilis, and was deposited at the China General Microbiological Culture Collection Center on November 10, 2023, with accession number CGMCC No. 28942. The fermentation supernatant, bacterial suspension, and cell-free extract of the porcine-derived Bacillus subtilis YB-X-721 of this invention all possess certain DPPH free radical, superoxide anion free radical, and hydroxyl free radical scavenging abilities, as well as a certain reducing ability, and can be used to prepare antioxidant products. Furthermore, when the porcine-derived Bacillus subtilis YB-X-721 of this invention is added to piglet feed as an animal feed additive, it can improve the growth performance of weaned piglets, significantly increasing average daily weight gain, and significantly reducing average daily feed intake, feed conversion ratio, and diarrhea rate.

The invention relates to a substance with a Prussian blue structure, which can generate hydroxyl radicals, superoxide anions and singlet oxygen in parallel under ultrasonic irradiation, and in the presence of ultrasonic irradiation and H2O2, the substance with the Prussian blue is used as a sonodynamic therapy (SDT) means to kill focus cells by generating sonotoxic and cytotoxic free radicals, so as to achieve the purpose of improving the therapeutic effect of the substance with the Prussian blue structure. Therefore, diseases are effectively treated.

The use of a cell-permeant molecular probe to assay the presence, and monitor the production, of the reactive oxygen species, superoxide, in red blood cells (RBCs) is described. The assay is performed using electron paramagnetic resonance (EPR) spectroscopy and a cell-permeant hydroxylamine spin trap. The cell-permeant molecular probe is not detected or quantifiable by EPR in its reduced form. However, upon entering the RBCs, the probe is oxidized by reacting with superoxide to yield a stable nitroxide radical that is detected and quantified using EPR. Our studies indicate that superoxide production is consistent with RBC metabolism that predicts lipid oxidation, membrane injury and poor post-transfusion performance. Therefore, measurement of reactive oxygen species can be used as an indicator of RBC quality at the time of donation and after storage.

The present invention belongs to the field of pharmaceutical chemistry technology, and specifically relates to a binuclear ruthenium photocatalyst, its preparation method, and application. The present invention discloses a novel tridentate Ru(II) complex for antibacterial purposes. The present invention discloses a binuclear ruthenium complex (see the structural formula below) that has a photocatalytic therapeutic effect against Staphylococcus aureus (S. aureus) and exhibits proliferation inhibition (MIC) against Staphylococcus aureus under light conditions. 90 6.25μM), and can generate superoxide anions and singlet oxygen under light conditions, and has photocatalytic oxidation ability on NADH / NADPH in bacteria. This is of great significance for the research of binuclear ruthenium complex antibacterial drugs and provides new ideas for the clinical development of binuclear ruthenium complex antibacterial photocatalytic drugs or antibacterial metal photosensitizers. #imgabs0#

The invention discloses a preparation method of a thermophilic thermophilic bacteria fermentation lysate, which comprises the following steps: inoculating a seed culture medium with thermophilic thermophilic bacteria for culturing to prepare a seed bacteria solution; inoculating the seed bacterial liquid into an initial culture medium for culturing, adding a supplementary culture medium when residual sugar reaches 4-6g / L, maintaining the residual sugar at 4-6g / L, and fermenting for 30-40 hours to obtain fermentation liquor; and filtering the obtained fermentation liquor by using a ceramic membrane, cleaning by using 7 times of water volume until the conductivity of the supernate is 100 [mu] s / cm, diluting the bacterial suspension to OD = 20, and homogenizing at high pressure for 1-3 times to obtain the Thermus thermophilus lysate. According to the method, the activity of superoxide dismutase in the thermophilic thermophilic bacteria lysate is improved, and the stability of nucleic acid in the thermophilic thermophilic bacteria lysate at normal temperature is improved.

The invention provides a gold-copper nanocluster with efficient multi-source active oxygen generation performance and a preparation method and application of the gold-copper nanocluster, and belongs to the field of biomedical materials. The gold-copper nanocluster is synthesized through mediation of Tf, and Tf is full transferrin, iron-saturated transferrin or siderophore. The gold-copper nanocluster with Tf as the ligand is synthesized by adopting a simple one-pot method, and the problems that in the prior art, a multi-source active oxygen generation system is complex to construct and a preparation method is tedious are solved; the gold-copper nanocluster synthesized by taking Tf as a ligand can be used as a simple multi-source active oxygen generation system, superoxide anions can be generated through photosensitized oxidation activity sensitization oxygen, and hydroxyl radicals can be generated through peroxidase-like activity catalysis hydrogen peroxide, so that efficient generation of multi-source active oxygen is realized; and the effect of enhancing tumor treatment is achieved.

This invention discloses a method for enhancing the high-temperature resistance of citrus through exogenous spraying of naringin, belonging to the field of agricultural technology. The method involves preparing naringin into a formulation and spraying it exogenously onto the leaves of citrus plants. Experiments show that after treatment with naringin, phenotypic damage such as wilting and curling of citrus seedling leaves under high-temperature stress is significantly alleviated; the maximum photochemical efficiency of photosystem II (Fv / Fm) is significantly increased; electrolyte leakage rate and malondialdehyde content are significantly reduced; and the accumulation of hydrogen peroxide and superoxide anions is effectively inhibited. This invention utilizes naturally sourced naringin to significantly enhance the tolerance of citrus to high-temperature stress through multiple mechanisms, including maintaining photosynthetic system function, protecting cell membrane integrity, inhibiting membrane lipid peroxidation, and scavenging reactive oxygen species. Furthermore, the method is simple to operate, environmentally friendly, and low-cost, making it suitable for stress-resistant citrus cultivation.