46 results about "Superoxide" patented technology

The invention belongs to the technical field of organic dye synthesis and application, and discloses a conjugated compound containing a benzothiophenazine structure as well as a preparation method and application thereof, and the conjugated compound containing the benzothiophenazine structure has excellent photophysical properties, singlet oxygen yield and superoxide anion generation capacity. The novel conjugated compounds ENBO-NI and ENBS-NI containing benzothiophenazine structures are successfully prepared by replacing a benzene ring of Nile blue with a cycloalkane structure and adjusting the electron donating ability of an R1 group. The two compounds have near infrared fluorescence emission, good solubility and relatively high ROS yield. Compared with a classical photosensitizer NB, the singlet oxygen yield of ENBO-NI is increased by about 117.65%, and the singlet oxygen yield of ENBS-NI is as high as 70%. The absorption wavelength of the conjugated compound is in a red light wave band. The preparation process is simple and efficient, large-scale production is easy, and the preparation method has a wide clinical application prospect.

The invention relates to the technical field of nano-materials, and discloses a heterogeneous diatomic nano-enzyme as well as a preparation method and application thereof. The invention provides a space confinement regulation and control strategy which is used for constructing a heterodiatomic catalytic center on a nitrogen-doped carbon skeleton and preparing nano-enzyme with structural stability and synergistic catalytic performance. According to the method, a confinement framework formed by self-assembly of first metal ions such as cobalt ions and second metal ions such as zinc ions and organic ligands is used as a template, third metal ions such as iron ions are introduced, and then carbonization heat treatment is performed in a protective gas atmosphere, so that bimetal sites with stable bridging configuration are formed in situ. The obtained nano-enzyme has the catalytic activity of various antioxidant enzymes, including catalase and remarkable superoxide dismutase activity, and has more excellent free radical scavenging capacity compared with a single-metal or non-bridging system.

This invention relates to a vehicle air conditioner, and the object of this invention is to improve the assemblability, maintenance, and repairability of a photocatalyst module by improving the intake housing structure, and to reduce air vortices generated at the air intake port side during air intake by the blower and the rumbling noise caused by these air vortices. To achieve this object, the vehicle air conditioner of this invention includes: an intake section that draws in internal and external air to blow them into the interior space of the vehicle; and a photocatalyst module that releases superoxide radicals into the air blown from the intake section into the interior space of the vehicle to purify the air, wherein the photocatalyst module is disposed on the outer surface portion of the downstream side of the blower housing of the intake section, and interference-avoidance recesses are formed on the outer surface portion of the intake housing corresponding to the periphery of the photocatalyst module disposed in the blower housing.

This invention discloses the application of a porcine-derived Bacillus in antioxidant activity, wherein the porcine-derived Bacillus is used to prepare antioxidant products. The porcine-derived Bacillus is Bacillus subtilis YB-X-721, classified as Bacillus subtilis, and was deposited at the China General Microbiological Culture Collection Center on November 10, 2023, with accession number CGMCC No. 28942. The fermentation supernatant, bacterial suspension, and cell-free extract of the porcine-derived Bacillus subtilis YB-X-721 of this invention all possess certain DPPH free radical, superoxide anion free radical, and hydroxyl free radical scavenging abilities, as well as a certain reducing ability, and can be used to prepare antioxidant products. Furthermore, when the porcine-derived Bacillus subtilis YB-X-721 of this invention is added to piglet feed as an animal feed additive, it can improve the growth performance of weaned piglets, significantly increasing average daily weight gain, and significantly reducing average daily feed intake, feed conversion ratio, and diarrhea rate.

The invention discloses a preparation method of a thermophilic thermophilic bacteria fermentation lysate, which comprises the following steps: inoculating a seed culture medium with thermophilic thermophilic bacteria for culturing to prepare a seed bacteria solution; inoculating the seed bacterial liquid into an initial culture medium for culturing, adding a supplementary culture medium when residual sugar reaches 4-6g / L, maintaining the residual sugar at 4-6g / L, and fermenting for 30-40 hours to obtain fermentation liquor; and filtering the obtained fermentation liquor by using a ceramic membrane, cleaning by using 7 times of water volume until the conductivity of the supernate is 100 [mu] s / cm, diluting the bacterial suspension to OD = 20, and homogenizing at high pressure for 1-3 times to obtain the Thermus thermophilus lysate. According to the method, the activity of superoxide dismutase in the thermophilic thermophilic bacteria lysate is improved, and the stability of nucleic acid in the thermophilic thermophilic bacteria lysate at normal temperature is improved.

This invention discloses a method for enhancing the high-temperature resistance of citrus through exogenous spraying of naringin, belonging to the field of agricultural technology. The method involves preparing naringin into a formulation and spraying it exogenously onto the leaves of citrus plants. Experiments show that after treatment with naringin, phenotypic damage such as wilting and curling of citrus seedling leaves under high-temperature stress is significantly alleviated; the maximum photochemical efficiency of photosystem II (Fv / Fm) is significantly increased; electrolyte leakage rate and malondialdehyde content are significantly reduced; and the accumulation of hydrogen peroxide and superoxide anions is effectively inhibited. This invention utilizes naturally sourced naringin to significantly enhance the tolerance of citrus to high-temperature stress through multiple mechanisms, including maintaining photosynthetic system function, protecting cell membrane integrity, inhibiting membrane lipid peroxidation, and scavenging reactive oxygen species. Furthermore, the method is simple to operate, environmentally friendly, and low-cost, making it suitable for stress-resistant citrus cultivation.

The invention relates to the technical field of catalyst preparation, and particularly discloses a non-stoichiometric selenium-doped Cu7.2 S4 photocatalyst based on carbon coating and a preparation method and application thereof, and the preparation method comprises the following steps: preparing copper-based metal organic framework precursor powder, then mixing the copper-based metal organic framework precursor powder with a selenium source and a sulfur source, and carrying out high-temperature post-vulcanization, selenylation and carbonization processes to prepare the photocatalyst. The photocatalyst prepared by the invention comprises selenium-doped Cu7. 2S4 and a carbon matrix material, wherein the carbon matrix material is coated on the surfaces of selenium-doped Cu7. 2S4 particles. The prepared photocatalyst has good visible light response, excellent carrier transport performance and good stability, can generate superoxide free radicals and hydroxyl free radicals under visible light irradiation, further shows excellent water pollutant photocatalytic degradation capacity and has good application prospects.

The present disclosure relates to methods of treating a range of diseases or conditions. The methods involve administration of a superoxide dismutase mimetic.

The application provides a method for improving the biological manganese oxidation performance of manganese-oxidizing bacteria and application, and belongs to the technical field of wastewater treatment.The method improves the biological manganese oxidation performance of the manganese-oxidizing bacteria by adding biochar, and after the biochar is added into the manganese-oxidizing bacteria, the addition of divalent manganese can obviously improve the oxidation rate of the divalent manganese and the removal efficiency of heavy metals in wastewater.The biochar can be used as an electron shuttle, can improve the electron transfer capacity and accelerate the oxidation rate of the divalent manganese, and the biochar also improves the gene abundance and expression capacity of the manganese-oxidizing bacteria and the extracellular superoxide concentration, improves the biological activity of the manganese-oxidizing bacteria and accelerates the oxidation of the divalent manganese.Further, the biochar is widely available and low in cost, can realize the reuse of resources and reduce the treatment cost, is easy to prepare and put into use and is convenient for use in practical application.

The present disclosure provides for imaging agents and methods of making and using the same. The imaging agent comprises a luminol component comprising a luminol moiety or functional analogue thereof; a chelator; a linker group bonding or complexing the luminol component to the chelator; and a radiolabel component bound to the chelator. The imaging agent described herein is capable of monitoring, targeting, or imaging oxidative stress, reactive oxygen species (ROS), superoxide generation, or hydrogen peroxide generation, mediating pathophysiology of different disease states.

This invention belongs to the field of pharmaceutical chemistry technology, specifically relating to a near-infrared II luminescent iridium(III) complex photosensitizer, its preparation method, and its application. The near-infrared II luminescent iridium(III) complex photosensitizer disclosed in this invention generates singlet oxygen (…) under 550 nm illumination. 1 O2), hydroxyl radicals (•OH), superoxide anions (O2) •‑ We evaluated the antitumor activity of near-infrared luminescent iridium complexes using mouse breast cancer cells (4T1). Under unilluminated conditions, the near-infrared II luminescent iridium(III) complex photosensitizers showed no significant cytotoxicity to 4T1 cells, while under illumination, they exhibited good phototoxicity to 4T1 cells, with an IC50 value of [missing value]. 50 The concentration is 15.92 μM. We have invented a near-infrared II luminescent iridium(III) complex photosensitizer, which has excellent photoimmunodynamic therapeutic effects and will have important application prospects in the treatment of hypoxic tumors.

The invention discloses a hydrogel microneedle loaded with a photo-thermal Fe3O4-MXene nano material as well as preparation and application of the hydrogel microneedle, and belongs to the field of biological medicines. The invention finds that a Fe3O4-Mxene nano material mainly takes CAT enzyme under a weak alkaline condition and can quickly remove hydrogen peroxide, Fe3O4 and TI3C2 form a unique heterojunction structure, the photo-thermal conversion performance of Ti3C2, the catalase activity of Fe3O4 and the superoxide dismutase activity of Fe3O4 are improved, and removal of Fe3O4 to active oxygen is promoted. The hydrogel microneedle loaded with the photo-thermal Fe3O4-MXene nano material, provided by the invention, is applied to diabetic, infectious and chronic wound surfaces, can remove excessive active oxygen, improves local oxygen deficit and promotes proliferation and regeneration of skin, and has a good application effect.

The invention discloses conjugated molecule-based donor-receptor two-phase self-assembled heterojunction nanoparticles and application thereof, and relates to the field of pharmaceutical preparations. A conjugated polymer is used as an electron donor, a conjugated small molecule is used as an electron acceptor, and an amphiphilic block copolymer is used as a surfactant, so that the heterojunction nanoparticles are formed through co-assembly. In the heterojunction nano-particles, the charge separation efficiency under illumination reaches up to 97%, so that a large amount of various kinds of active oxygen including singlet oxygen (1O2), hydroxyl free radicals (. OH) and superoxide anions (O2.-) are generated, and the antibacterial and anti-tumor purposes are achieved. Besides, due to the special structural design, melanin can participate in a photocatalytic reaction, generation of active oxygen is further promoted, the killing capacity on melanin toxic tumors is improved, and a new direction is provided for developing novel nano-drugs with efficient quantum yield for antibacterial and anti-tumor treatment.

This invention discloses the application of *Pteris vittata* oil in skincare products. The preparation method of the *Pteris vittata* oil involves first extracting the raw material using supercritical CO2 extraction technology to obtain crude *Pteris vittata* oil; then purifying the crude *Pteris vittata* oil through multi-stage molecular distillation, collecting the light fraction to obtain the final *Pteris vittata* oil. This preparation method can efficiently enrich the antioxidant active ingredients such as tocopherols, squalene, and polyunsaturated fatty acids in the *Pteris vittata* oil. The prepared *Pteris vittata* oil exhibits excellent scavenging ability against DPPH free radicals, superoxide anions, and ABTS free radicals, while significantly enhancing SOD activity, reducing MDA levels, and minimizing oxidative stress damage, thereby exerting antioxidant and anti-aging effects. This provides a safe, gentle, and highly effective natural raw material for anti-aging, whitening, and skin barrier repair skincare products, and has promising market application prospects.

The application discloses an osmium complex, a preparation method thereof and application of the osmium complex in preparation of a medicine for treating endometriosis. The complex has a near-infrared light catalytic treatment effect on human endometrial ectopic cells (12Z), can generate superoxide anions and singlet oxygen under irradiation of a 700 nm light source, and has catalytic oxidation ability on NADH / NADPH in the 12Z cells. The series of complexes have good proliferation inhibition ability (IC 50 <40 μM) on the 12Z cells, and the proliferation inhibition ability on the 12Z cells is enhanced with optimization of a ligand structure, which has important significance for research on a new medicine for treating endometriosis and provides a new idea for developing a photodynamic treatment medicine for endometriosis in a clinic.

The superoxide micro-nano bubble generator comprises a supporting table and a bubble spraying pipe, an extension pipe is fixedly connected to the upper portion of the bubble spraying pipe, a liquid sliding ring is fixedly connected to the upper end of the extension pipe, and the fixed end of the liquid sliding ring is fixedly connected to the gas outlet end of a bubble generator through a pipeline. A driving assembly is fixedly connected below the supporting table, a shearing assembly is fixedly connected into the bubble spraying pipe, bubble spraying openings are formed in the two sides of the bottom of the bubble spraying pipe, an electric sliding ring fixing end is fixedly connected to one side of the liquid sliding ring fixing end, a first power supply circuit is fixedly connected to one side of the electric sliding ring fixing end, and an external power source is connected to the other end of the first power supply circuit. The rotating motor drives the bubble spraying pipe to rotate, the transmission gear set is combined with the crown gear to drive the shearing assembly to shear bubbles at a high speed, and the gas-liquid contact area is increased. And meanwhile, the coverage range is expanded through rotary spraying, and the problem of non-uniform traditional static spraying is solved.

This disclosure provides a method for alleviating aluminum toxicity in hydroponic peas based on menadione-mediated reactive oxygen species (ROS) metabolism. The core components include: regulating the concentration and duration of menadione treatment to optimize the spatiotemporal accumulation pattern of ROS (superoxide anion, hydrogen peroxide) in the roots of hydroponic peas; adjusting the dynamic response of endogenous antioxidant enzyme systems (SOD, POD, APX) to enhance menadione-mediated ROS scavenging capacity; controlling the initial ROS burst based on changes in plasma membrane NADPH oxidase (Rboh) gene expression and enzyme activity to inhibit the aluminum stress oxidative signaling cascade; and adjusting the timing and frequency of menadione administration based on subcellular-scale mitochondrial and chloroplast ROS leakage rates to alleviate organ-specific oxidative damage. This approach addresses five key issues: aluminum-induced oxidative damage, cellular redox imbalance, oxidative signaling cascade, organ-specific oxidative damage, and persistent aluminum toxicity metabolic disorders.

The application discloses an electrode material based on a non-haem iron catalyst and a preparation method and application thereof, and belongs to the field of electrocatalytic water treatment. The electrode material is used as a substrate, and an auxiliary ligand polymer layer and a non-haem iron catalyst layer are sequentially deposited on the substrate; the auxiliary ligand polymer layer is polymerized from organic monomers; the non-haem iron catalyst layer is formed by reaction of a ferrous salt and dopamine to form a precursor and then electrochemical polymerization; and the auxiliary ligand polymer layer and the non-haem iron catalyst layer are connected through coordination bonds. The auxiliary ligand polymer layer and the non-haem iron catalyst are electrochemically polymerized on the electrode material, so that the selectivity of the electrode material for electrocatalytic oxygen two-electron reduction to produce hydrogen peroxide is improved, and the hydrogen peroxide yield is increased. In addition, the non-haem iron catalyst can further produce superoxide free radicals through regulation of single-electron reduction of oxygen, promote the generation of hydroxyl free radicals from hydrogen peroxide, and maintain good electrocatalytic removal efficiency of organic pollutants under near-neutral conditions.

This invention belongs to the field of fluorescent probe technology, specifically relating to a fluorescent probe for nuclear-targeted detection of superoxide anions, its preparation method, and its application. The structural formula of the fluorescent probe is shown below. This fluorescent probe can visualize the dynamic changes in ROS concentration in the cell nucleus in situ in real time, which is of great significance for revealing the pathological mechanisms of oxidative stress-related diseases. It helps to advance the research on ROS-related signal transduction and gene regulation pathways in the cell nucleus and the early diagnosis of diseases, providing a reliable basis for clinical pathological detection.

This invention belongs to the field of agricultural stress resistance technology, and specifically relates to a compound regulator for alleviating maize kernel abortion under heat stress, its application, and method. This invention provides a compound regulator for alleviating maize kernel abortion under 35-40℃ heat stress. This compound regulator is prepared by adding salicylic acid, choline chloride, and potassium dihydrogen phosphate to water in a certain ratio. Spraying the compound regulator onto the entire maize plant leaves significantly increases the main axis length, number of florets on the main axis, and density of florets on branches under heat stress, improves pollen and silk viability, and reduces the number of aborted kernels; it also significantly increases ear length, ear diameter, number of kernels per row, number of kernels per ear, and 100-kernel weight. Furthermore, this compound regulator can also reduce the content of malondialdehyde (MDA) and superoxide anion free radicals (O2) in leaves under heat stress. ‑ It can improve the production rate of chlorophyll, delay the degradation of chlorophyll and leaf senescence in maize, prolong the green period of leaves, thereby effectively alleviating heat stress in maize, increasing dry matter accumulation and yield, and has broad application prospects.

The utility model discloses a superoxide molecule waste gas purification system which comprises a reaction tower, a gas inlet pipe is fixedly connected to the interior of the reaction tower, a first pump body is fixedly connected to the outer wall of the reaction tower, a first flow dividing pipe is fixedly connected to the output end of the first pump body, a first spraying pipe is fixedly connected to the first flow dividing pipe, and a second spraying pipe is fixedly connected to the second spraying pipe. A first pump body is fixedly connected to the outer wall of the reaction tower, a first connecting pipe is fixedly connected to the input end of the first pump body, a second pump body is fixedly connected to the outer wall of the reaction tower, a second flow dividing pipe is fixedly connected to the output end of the second pump body, a second spraying pipe is fixedly connected to the second flow dividing pipe, and a second connecting pipe is fixedly connected to the input end of the second pump body. The device can adapt to treatment of waste gas of any type and any concentration, is adjustable in air volume during treatment, has the advantages of low power consumption, simplicity in installation, small occupied area, high purification efficiency, long service life and the like, and realizes real non-toxic and harmless emission.

Provided are compositions comprising super-oxide dismutase and a soluble fiber. The compositions may additionally comprise other antioxidants, sweeteners, probiotics, vitamins and nutrients. The compositions can be used as dietary supplements and for improving health and well-being. Also provided are methods of using such compounds.

The invention discloses amino-functionalized Nile blue dye as well as a synthesis method and application thereof, and belongs to the field of organic synthesis. According to the method, by optimizing the catalyst type, the reaction temperature and the solvent system, the generation of isomer byproducts is effectively inhibited, and the reaction selectivity is improved. Compared with the prior art, the synthesis process disclosed by the invention has the advantages of simple reaction steps, mild and controllable conditions, high product yield and high purity; meanwhile, large-scale use of strong acid, strong alkali and expensive noble metal catalysts is avoided, and the production cost is remarkably reduced. The obtained amino-functionalized Nile blue dye has long wavelength absorption, excellent cell membrane permeability and wide pH tolerance range (pH 2-11), and can generate superoxide anion free radicals under red light excitation. Based on the characteristics, the compound can be widely applied to the fields of biological fluorescence labeling and functional material preparation.

The application discloses a 7-azaindole photoacoustic imaging probe for detecting superoxide anion, a preparation method and application, and the photoacoustic imaging probe is named AIH-OTF, which is a blue-green solid prepared from methyl trifluoromethanesulfonate, dye AIH-OH and the like; the AIH-OTF realizes high-contrast PA imaging of endogenous superoxide anion in living cells, and in-situ evaluation of occurrence and repair of acetaminophen-induced liver injury is also determined through non-invasive monitoring of the PA reaction of AIH-OTF to superoxide anion; the AIH-OTF probe can be used as a useful tool for accurately diagnosing drug-induced liver injury in vivo. The photoacoustic imaging probe can realize real-time detection of in-vivo superoxide anion through AIH-OTF and the ability of diagnosing DILI; the cell toxicity is small, the biological compatibility is good, and the photoacoustic imaging probe can be used for in-vivo imaging of occurrence and repair of DILI.

The application discloses an iridium complex and a preparation method and application thereof, and the iridium complex comprises a structure shown in the following formula: wherein X ‑ represents an anion. The compound of the application scheme has a spontaneous light emitting characteristic in an alkaline environment, can generate superoxide anions and singlet oxygen, and has catalytic oxidation ability on NADH / NADPH in tumor cells. The complex has good proliferation inhibition ability (IC 50 50 of 3.67 muM) on human non-small cell lung cancer cell lines (A549) under normal oxygen conditions, and also has proliferation inhibition ability (IC 50 50 of 6.85 muM) under anoxic conditions, which has important significance for researching the anti-tumor of the spontaneous light emitting metal iridium complex and provides a new idea for developing the photodynamic therapy of deep tumors in the clinic. In the complex of the application scheme, the donor and the acceptor are covalently attached, the covalent interaction is more stable, and the distance is closer, and therefore, higher transfer efficiency can be achieved.

The present disclosure relates to methods of treating a range of diseases or conditions. The methods involve administration of a superoxide dismutase mimetic.

The present application relates to a kind of side chain modified pentamethine cyanine dye and its synthesis method and application.The dye has general formula CY5-R, the dye has strong absorption and emission characteristics above 650nm, suitable for red light excitation, mitochondrial localization and type I photodynamic therapy.Experiments show that the dye and mitochondrial co-localization coefficient reaches 0.860, exhibit excellent deep tissue penetration ability and low dark toxicity, after illumination IC 50 0.15 μM, can efficiently generate superoxide anion free radical, significantly kill cancer cells, realize diagnosis and treatment integration.The synthesis method of the present application includes quaternary ammonium salt synthesis, condensation reaction and side chain modification and the like, with the characteristics of cheap raw material, high yield, simple operation.The dye has wide application in photocatalysis, photodynamic therapy, biomarker and deep tissue imaging and the like, especially suitable for deep tumor fluorescence imaging and photodynamic therapy.

The invention belongs to the field of organic matter preparation, and particularly relates to a two-photon fluorescent nanoprobe for detecting superoxide anions in glioma as well as a preparation method and application of the two-photon fluorescent nanoprobe. A compound containing a naphthalene ring, a trifluoromethanesulfonate group and a cyanovinyl group is prepared as a nanoprobe, and then the nanoprobe is compounded with transferrin to form a delivery platform. The two-photon fluorescent nanoprobe for detecting superoxide anions in glioma and a delivery platform prepared from the probe have strong lysosome targeting imaging property and have an effect of crossing a blood brain barrier, and precise boundary description beyond traditional anatomical imaging is realized.

The application provides a coordination nanometer assembly of a chiral cyclic peptide and a manganese ion, a preparation method and application thereof, the method of the application combines a manganese superoxide dismutase protein domain with a unique tumor microenvironment of a melanoma high tyrosinase, and first designs polypeptide sequences with different chirality L‑ Y D‑ h L‑ D D‑ h、 L‑ Y L‑ H L‑ D L‑ H and D‑ y D‑ h D‑ d D‑ h, regulates polypeptide chirality, improves in-vivo circulation stability and cell entry efficiency of the nanometer assembly, coordinates self-assembly of manganese and the chiral cyclic peptide, simulates in-situ oxidation of the tumor microenvironment for photothermal therapy, and coats cell membranes. The method of the application is simple, the experimental conditions are mild, and the method is easy to operate, the cell membrane coated nanometer assembly prepared by the method not only has long circulation stability and high cell entry efficiency, but also can realize mild photothermal therapy through in-situ oxidation, release manganese ions to activate the CGAS-STING pathway for tumor immunotherapy, and has potential application value in the field of tumor combination therapy.

This invention discloses a cryopreservation diluent containing fucoxanthin and its applications. Adding fucoxant to the cryopreservation diluent enhances the antioxidant capacity of frozen-thawed semen, reduces oxidative damage to goat sperm from reactive oxygen species during freezing-thawing, significantly improves sperm viability, motility, plasma membrane integrity, and total antioxidant capacity after freezing-thawing, enhances catalase and total superoxide dismutase activity, and reduces reactive oxygen species and malondialdehyde content. The effect is best when the fucoxant concentration is 2 μM during cryopreservation. The cryopreservation diluent of this invention is simple to prepare and operate, significantly improves the cryopreservation effect of semen, and has important practical application value.