55 results about "Catalase T" patented technology

A multifunctional membrane is provided. The multifunctional membrane is suitable for use in an analyte sensor. In a particular application, the multifunctional membrane may be used in connection with an amperometric biosensor, such as a transcutaneous amperometric biosensor. Some functions of the membrane are associated with properties of membrane itself, which is comprised of crosslinked polymers containing heterocyclic nitrogen groups. For example, the membrane, by virtue of its polymeric composition, may regulate the flux of an analyte to a sensor. Such regulation generally improves the kinetic performance of the sensor over a broad range of analyte concentration. Other functions of the membrane are associated with functional components, such as a superoxide-dismutating/catalase catalyst, either in the form of an enzyme or an enzyme mimic, that can be bound to the scaffold provided by the membrane. The effect of any such enzyme or enzyme mimic is to lower the concentration of a metabolite, such as superoxide and/or hydrogen peroxide, in the immediate vicinity of the sensing layer of the biosensor. Lowering the concentrations of such metabolites, which are generally deleterious to the function of the sensor, generally protects or enhances biosensor integrity and performance. The membrane is thus an important tool for use in connection with analyte sensors, amperometric sensors, biosensors, and particularly, transcutaneous biosensors. A membrane-covered sensor and a method for making same are also provided.

The present invention provides a composition, comprising: greater than about 0.1% by weight hydrogen peroxide; an aromatic acid component; surfactant; optionally, a solvent; and a carrier. The composition of the invention is useful as a disinfecting composition for killing microorganisms such as bacterium (including Mycobacterium), spores and fungi. The composition provides a pathogenic bacteria kill rate of 99.9% in about 30 seconds when bacteria are exposed to the composition and is effective in providing a Mycobacterium kill of 10⁶ with two minutes or less. Moreover, the compositions of the invention are generally more resistant to catalase deactivation than, for example, an aqueous solution of hydrogen peroxide. The concentration of hydrogen peroxide within the composition may range from about 1% by weight to about 7% by weight and the concentration of aromatic acid component may range from about 0.1% by weight to about 5% by weight. The invention also provides a method for disinfection of a substrate utilizing the composition. The composition of the invention may be used in the foregoing method on a medical instrument, such as an endoscope or the like. Applying the compositions to a substrate may be accomplished in any of a variety of application methods such as by roll coating, dipping, spraying, or rotational tumbling. The composition may be applied to the substrate for a period of time ranging from about 30 seconds to about ten minutes. In this aspect, the invention can further comprise drying the substrate after removing the composition.

An oxygen releasing bandage or dressing is based on a stable complex between polyvinyl acetal and hydrogen peroxide. Medical grade polyvinyl acetal sponge is treated with hydrogen peroxide, thereby forming a complex between the acetal plastic and the hydrogen peroxide. The dressing material is then affixed to a wound preferably with an oxygen impermeable covering. Fluids from the wound are drawn into the sponge where catalase and other enzymes in the fluids breakdown the hydrogen peroxide to release oxygen. If treatment of relatively dry wounds is desired, catalyst solutions can be introduced into the bandage to stimulate production of oxygen. The bandages are stable for a prolonged period of time.

Single amino acid compounds and methods for upregulating expression of a gene encoding an antioxidative enzyme, such as superoxide dismutase or catalase, to counteract harmful oxidative effects of reactive oxygen species and other free radicals are described. The single amino acid compounds may be used in compositions and methods to treat or prevent diseases and conditions characterized by undesirable elevation of reactive oxygen species and other free radicals.

In some embodiments of the apparatus detects the concentration of a substance selected from the group consisting of hydrogen peroxide, peroxyacetic acid, chlorinated compounds and other aqueous oxidizer compounds. The light source may be a laser, or light emitting diode. The source may be part of a transmitter and the detector may be part all of a receiver. The apparatus may further include a data logger cooperating with the light detector. The enzyme coated strip may be a strip coated with catalase and the the strip may be an inert paper. In some cases the enzyme coated strip changes color as a function of the concentration of the substance. The apparatus may further including fiber-optic cable cooperating with said light detector to detect transmitted light from the enzyme coated strip and a fiber-optic cable and/or a fiber optic cable cooperating with said source to direct light on the enzyme coated strip. The invention also includes the method for measuring the concentration of a substance which includes providing an enzyme coated strip, projecting a light beam on the enzyme coated strip, and detecting light from the enzyme coated strip.

Conversion in vitro of X-Gly to X-alpha-hydroxy-Gly or X—NH₂ (X being a peptide or any other compound having a carbonyl group capable of forming a covalent bond with glycine) is accomplished enzymatically in the presence of keto acids, or salts or esters thereof, to provide a good yield without the necessity of catalase or similar enzymatic reaction enhancers. Peptidylglycine α-amidating monooxygenase (PAM) is a preferred enzyme for catalyzing the conversion. Alternatively, peptidylglycine α-hydroxylating monooxygenase (PHM) is utilized to convert X-Gly to X-alpha-hydroxy-Gly which may be recovered, or optionally may be simultaneously or sequentially converted to an amide by either a Lewis base or action of the enzyme peptidyl α-hydroxyglycine α-amidating lyase (PAL). Both PHM and PAL are functional domains of PAM.

The invention provides a carbendazim pesticide residue degrading bacteria and a prodegradant, which belongs to biological high-tech domain. The used bacterial strain DJL-6 is judged as Rhodococcus sp. The main biological feature is: G+, the thallus is of ball rod and atrichosis; the reaction between catalase and V-P is positive; the oxidase reaction is negative; the barley sugar, carubinose, citrate, acetamide and agedoite can be used as unique carbon source or nitrogen source to carry out its growth, but the lactin and bitter almond acid can not be used as unique carbon source to carry out its growth; and the bacterial colony is orange when cultivated on LB culture medium. The pesticide residual quantity of the crop can be reduced by more than 95 % by directly applying the degrading bacteria product, which solves the overproof pesticide residue problem, and can produce non-toxic and nuisanceless environmental farm product.

A method for treating ship ballast water in which organisms viable in the ship ballast water are exterminated by adding to the ship ballast water hydrogen peroxide or a compound producing hydrogen peroxide in an amount such that a hydrogen peroxide concentration comes to be 10 to 500 mg/L and at least one type selected from a ferrous ion or a compound supplying ferrous ion in an amount such that a ferrous ion concentration comes to be 0.1 to 400 mg/L, catalase in an amount such that a concentration of catalase comes to be 0.5 to 2,500 unit/L and iodine or a compound supplying iodine amount such that an iodine concentration comes to be 0.1 to 100 mg/L.

The invention discloses a detection kit for LDL (low-density lipoprotein) cholesterol and a use method of the detection kit, relates to the field of biochemical detection and aims to provide a detection kit, having high stability, accuracy and precision as well as low toxicity, for LDL cholesterol and a use method of the detection kit. The kit comprises a reagent 1 and a reagent 2, wherein the reagent 1 is prepared from a surfactant, polyethylene glycol, SDS (sodium dodecyl sulfate), Emulgen A9 series, CHOD (cholesterol oxidase), CHER (cholesterol esterase), ascorbic acid oxidase, CAT (catalase), 4-AAP (4-aminoantipyrine) and the like; the reagent 2 is prepared from octylphenyl polyethylene glycol, cholate, a compound stabilizer, bovine serum albumin, POD (peroxidase), TOPS (sodium 3-(N-ethyl-3-methylanilino)propanesulfonate) and the like. The proclin series added to the kit is a novel biological preservative, has good compatibility with various enzymes, better stability and low toxicity, and the stability of the reagents is maintained.

The invention discloses a highly sensitive paper-based photoelectrochemical sensor for detecting microRNA. A paper chip is prepared by means of a wax printing technology, gold nano-particles are grownin situ in a hydrophilic working area to achieve functionalization of the paper chip, a cuprous oxide/bismuth sulfide/bismuth vanadate tertiary sensitizer is modified subsequently, and microRNA is captured through a fixed hairpin DNA chain; by means of identification of specificity and digestion effect of duplex-specific nuclease, signal amplification on microRNA is achieved, and then by means ofa multibranched hybrid chain, platinum nanoparticles are embedded into a main part of the chain, branch parts form a chlorhematin/G-tetrad structure, and a DNA concatemer with catalase bological characteristics is formed; signal amplification is further achieved, thus preparation of the photoelectrochemical sensor is completed, and highly sensitive and accurate detection on microRNA is achieved.

The invention relates to an environmental protection technical field, in particular to a method for the early detection of the heavy metal pollution in air by taking the moss catalase as a marking object; the steps of the method are as follows: firstly mosses are collected, and the catalase is extracted from the mosses, finally the catalase is analyzed and the extent of the heavy metal pollution in air is judged according to the activity of the catalase. Because the activity of the catalase of the mosses is analyzed and the extent of the heavy metal pollution in air is judged according to the activity of the catalase, the invention has the advantages of flexibility, high efficiency, easy operation and low cost; the method has good early warning effect for the heavy metal pollution in the air, and is particularly applied to conditions in which pollutants are too low to cause morphological changes of plants.

Methods and compositions are provided for determining ADP in the presence of ATP. These comprise including among the assay reagents at least one of the correcting components creatine phosphokinase and phosphocreatine, pyruvate kinase and phosphoenolpyruvate, peroxidase and a non-interfering peroxidase substrate, and catalase. One aspect of the method employs formation of hydrogen peroxide from the ADP by pyruvate kinase, phosphoenolpyruvate and pyruvate oxidase. The hydrogen peroxide is then determined. A combined reagent having all of the reagents may optionally include a peroxidase when the hydrogen peroxide is to be enzymatically determined. A peroxidase substrate is added to the sample in conjunction with the peroxidase substrate reagent, the mixture incubated and depending on whether the peroxidase substrate is a fluorescer or chemiluminescer, the mixture may be illuminated with excitation light and the emitted light determined as a measure of the ADP in the sample.

A highly safe, inexpensive and widely utilizable method, which has a mechanism backed by scientific grounds, for improving an activity of ROS (reactive oxygen species)-scavenging enzyme group; An increase in the amount of an enzyme or promotion of an enzyme activity of the ROS scavenging enzyme group such as superoxide dismutase, catalase or peroxidase is caused in an organism having the ROS-scavenging enzyme with one or two or more kinds of substances selected from the group consisting of erythritol, mannitol, sorbitol and xylitol in 0.01-10% administration concentration.

The invention relates to a calorimetric method for detecting pesticides by using acetylcholin esterase. According to the technical scheme, an enzymatic inhibition method and cascade reaction are adopted; the acetylcholin esterase is suppressed by pesticides, so that the enzymatic reaction rate is reduced; the quantity of choline products is reduced and is in direct proportion to the suppression degree; and furthermore, the amount of the choline can be obtained by cascade reaction heat of choline oxidase and catalase. By using the cascade reaction, the intensity of an original thermal signal as well as the detection sensitivity and the detection resolution are greatly improved, the detection limit is reduced, and the detection accuracy is effectively improved; a pesticide detection calorimetric method based on the acetylcholin esterase is realized; the advantages of high sensitivity of the acetylcholin esterase to organophosphorus and carbamic acid ester type pesticides and no interference caused by electrochemical active substances or colors and turbidity of the sample on the calorimetric method are integrated; the in-site quick pesticide residue detection is facilitated; and the large-scale application is facilitated.

The invention relates to a method for detecting activity of catalase, belonging to the field of enzymatic activity detection methods. The method for detecting activity of catalase comprises the following steps: diluting a catalase standard substance into a standard solution with a series of catalase activity concentrations, adding 10mL of chlorine peroxide phosphate buffer solution in a 100mL iodine measuring flask, putting the iodine measuring flask in water bath at 25 DEG C and carrying out heat preservation, then adding 2mL of a standard enzyme solution, accurately carrying out heat preservation for 3 minutes, instantly adding 2mL of 0.5mol/L sulfuric acid to end reaction, adding 2mL of 0.5mol/L sulfuric acid into a contrast tube of a contrast reaction solution to end reaction, and then adding the enzyme solution; then extracting 0.1mL of the reaction solution, adding 2.9mL of a peroxidase-dianisidine solution in a test tube and shaking to be uniform, firstly carrying out zero adjustment by a blank test tube in 436microns by virtue of a spectrophotometer at 25 DEG C, then detecting absorbancy of each reaction solution test tube to obtain a curve about the relation between the standard enzymatic activity and the absorbancy. The test enzyme is processed by the same method, so that the enzymatic activity of the test enzyme can be obtained according to the absorbancy and the standard curve. The detection method has the characteristics of being accurate, high in practicability, simple in instrument requirement and suitable for operation in the conventional condition; the detection method belongs to one reliable method of color rendering methods for detecting the activity of the catalase.

The invention discloses a process for preparing gold nano particles through reduction of chloroauric acid by catalase. The process comprises the steps of dropping a catalase solution into a chloroauric acid solution, and mixing uniformly; and adjusting the pH value of a mixed solution to be alkaline, reacting on the water bath condition of 20-37 DEG C, and obtaining gold nano particles through separation after the reaction is completed. According to the process, the catalase is introduced to serve as a reducing agent and a protective agent, reducing functional groups on the catalase are high in reducibility on the alkaline condition, the synthesis of gold nano particles is facilitated, and the produced gold nano particles are free from agglomeration on the high salinity condition (0.5MNaCl).

The invention provides a method for raising disease resistance of paddy rice to rice blast by the utilization of alginooligosaccharide. By spraying alginooligosaccharide during the rice seedling stage, activity of defensive enzymes--phenylalnine ammonialyase, catalase and peroxidase in the rice leaves is raised, and furthermore disease resistance of paddy rice to rice blast is enhanced. By the method, disease resistance of paddy rice is enhanced, and growth of paddy rice is promoted. Meanwhile, the method is simple and feasible and is beneficial to agricultural promotion and application.

The invention discloses a visualized enzyme linked immunoassay method which comprises the following steps: (1) Au NBP@Ag nanorod preparation; (2) enzyme-linked immunoassay. According to the visualized enzyme linked immunoassay method, a primary antibody has been laid on a plate, antigen is added, then catalase modified secondary antibody compound is added, and then Fe2<+> and hydrochloric acid are added after the equivalent of hydrogen peroxide is added; then a silver plated gold cone nanorod is added to react, a series of fresh colors are observed in an etching process, and a target antigen is analyzed visually and semi quantitatively. The visualized enzyme linked immunoassay method disclosed by the invention can generate varieties of color change, solves the defect that color change in a traditional colorimetric method is single, achieves quantitative detection on a target object and further has quick reaction time and a good effect; thus, accuracy of the method for naked eye detection is greatly improved, and a detection limit is lower.

A method for stabilizing particulate peroxycarboxylic acids, in particular imidoperoxycarboxylic acids, (such as, e.g., PAP), which are solid at an ambient temperature in a preferably aqueous dispersion containing surfactants. The dispersion is established in such a way that in the dispersed state a degradation of the peroxycarboxylic acids in the dispersion is prevented or at least reduced or retarded, or that the solubility of the peroxycarboxylic acids in the dispersion is diminished, in particular by minimizing the halide ion content, reducing the pH value to pH values ≦7, minimizing the content of free or active surfactants, minimizing the content of non-ionic surfactants, adding complexers, adding catalases or adding a solvent with a low solubility capability for peroxycarboxylic acids etc.

Peptide compounds and methods for upregulating expression of a gene encoding an antioxidative enzyme, such as superoxide dismutase or catalase, to counteract harmful oxidative effects of reactive oxygen species and other free radicals are described. The peptide compounds may be used to treat or prevent diseases and conditions characterized by undesirable elevation of reactive oxygen species and other free radicals, to upregulate AP-1 gene expression, and to treat pain. The peptide compounds may be used as components of pharmaceuticals and dietary supplements.

The invention is directed to a process for removing chlorite ion from a body of water containing unacceptably high levels of chlorite comprising adding to said body of water a chlorite removal chemical selected from the group comprising sodium dichloroisocyanurate dihydrate, sodium dichloroisocyanurate, trichloroisocyanurate, polyaluminum chloride, sodium permanganate, potassium permanganate, and catalase enzyme.

The molecular mechanisms of peroxisome biogenesis have begun to emerge: in contrast, relatively little is known about how the organelle functions as cells age. The present inventors characterized age-related changes in peroxisomes of human cells and showed that aging compromises peroxisomal targeting signal 1 (PTS1) protein import, with the critical antioxidant enzyme, catalase, especially affected. The number and appearance of peroxisomes are altered in these cells, and the organelles accumulate the PTS1-import receptor. Pex5p, on their membranes. Concomitantly, cells produce increasing amounts of the toxic metabolite, H₂O₂, and this increased load of reactive oxygen species (ROS) may further reduce peroxisomal protein import and exacerbate the effects of aging. Disclosed are novel compositions and methods for restoring catalase in peroxisomes by use of targeted catalase modified at its C-terminus and/or N-terminus, optionally in combination with polypeptides which promote cellular uptake of proteins, to prevent or overcome the changes that follows aging or that are associated with a number of diseases or disorders.

The invention relates to an analytical method of ecological effect influence of domestic-garbage-leachate-contaminated soil, comprising the following steps: 1. adjusting moisture contents of a contaminated soil sample and an uncontaminated soil sample to be 60% of field moisture capacity and culturing at the temperature of 20-30 DEG C; 2. selecting microbial activity indexes (respiratory intensity and soil enzymatic activity(catalase, amylase, urease and invertase)) and biotoxicity indexes (protozoon fatality rate) as ecological effect indexes of a soil sample; 3. comparing according to the difference of ecological indicators of the contaminated soil sample and the uncontaminated soil sample and analyzing the contaminated degree of the soil by the domestic garbage leachate. Indexes monitoring referred in the analytical method disclosed by the invention is convenient to operate and is easy to carry out, and can be a supplement for chemical index monitoring.

An amperometric sensor for uric acid and a manufacturing method thereof are disclosed, in which polyacrylamide is used to fix catalase, uricase and ferrocenecarboxylic acid on a working electrode. In determining concentration of uric acid, hydrogen peroxide is produced when enzyme and uric acid react with each other and then a reduction current generated from enzyme on the electrode with an external voltage 200 mV applied is detected. In determining concentration of uric acid, a concentration range of 2.5-20 mg/dl is achieved and sensibility of the sensor in a linear portion is 5.17 uAcm⁻²(mg/dl)⁻¹. In addition, reaction time required for the reaction between enzyme and uric acid is 5.17 uAcm⁻²(mg/dl)⁻¹.