7 results about "Concatemer" patented technology

Provided are methods and compositions used for paired-end sequencing. The method comprises providing a DNA array comprising a surface immobilized with DNA concatemers. Foreach of the plurality of DNA concatemers on the array, the method comprises annealing first read primers to primer binding sites on the DNA concatemer, extending at least some of the first read primers to incorporate dNTPs or dNTP analogs, thereby producing first read strands, each of the dNTPs or dNTP analogs being incorporated is identified to produce first reads, performing controlled MDA by extending at least some of the first read strands with a polymerase having strand-displacement activity to generate a plurallty of second strands, each second strand comprising a portion that is hybridized to the DNA concatemer, and an unhybridized, single-stranded branch; and annealing second read primers to the single-stranded branches of the plurality of second strands, and extending the second read primers to generate the second reads.

The invention relates to a method and a kit for generating nucleic acid for target capture. The method involves binding of a circ-olio sequence to at least one target oligonucleotide to generate a single stranded circular DNA. The single stranded circular DNA is bidirectionally amplified with the sense and antisense oligonucleotides, followed by biotinylation to obtain for a double-stranded concatemer of linear sequence. The double-stranded concatemer of linear sequence with the restriction endonucleases produces a target capture nucleic acid. The method offers a scalable targeted capture of pr eselected genomic regions, and is cost effective. The method of generating nucleic acid for target capture has application in the field of oncology hematology, inherited malignancies, germline disorders, microbiology and prenatal diagnosis. The invention generates baits over high GC-rich regions of the genome.

The present disclosure in some aspects relates to methods and compositions for accurately detecting and quantifying multiple analytes present in a biological sample. In some aspects, the methods and compositions provided herein address one or more issues associated with the stability and / or size of nucleic acid structures, such as RCPs, in the biological sample without the use of exogenously added oligonucleotide compaction probes. In some embodiments, provided herein are methods involving the use of self-hybridizing hybridizing regions for compacting and / or stabilizing nucleic acid concatemers (e.g., RCPs). In some embodiments, dynamic inter-strand annealing between tandem units of an RCP is used for compaction and / or stabilization. In some embodiments, short palindromic regions in an RCP are used for compaction and / or stabilization.

The present disclosure provides methods for reducing sequencing errors comprising one or any combination of: (i) removing deaminated bases in any nucleic acid molecule throughout a library preparation workflow which includes immobilised splints which bind to the library, the use of a compaction oligonucleotide, optionally with an intervening sequence, formation of closed circular nucleic acids, creating gaps using glycosylase and lyase activities at positions with deaminated bases. The library may be sequenced using pairwise sequencing, e.g. with dark sequencing and / or sequencing using a multivalent labelled probe for the formation of an avidity molecule and soluble primer and polymerase. Method for sequencing concatemers in which the concatermers are contacted with polymerases, soluble primers and a multivalent labelled molecule which forms a complex with the polymerase. Detecting polymerase position and nucleobase bound to the polymerase in the complex. These methods generate higher quality base calls during downstream sequencing workflows.

Provided herein are systems and methods for amplifying nucleic acid samples, immobilizing such amplified products onto substrates, and sequencing the amplified products on the substrates. The amplified products may comprise concatemers.

The application provides a method for detecting the purity of supercoiled DNA in plasmid samples with different molecular weights and application thereof, and comprises the following steps: preparing linearized plasmid samples and untreated plasmid samples for HPLC detection; performing HPLC detection by using an anion exchange column; collecting chromatograms of supercoiled plasmid DNA, open circle plasmid DNA, linear plasmid DNA and concatemer plasmid DNA in linearized and untreated plasmid samples with different molecular weights; and the mobile phase of the HPLC detection comprises: mobile phase A is a water solution containing tris-hydroxymethyl aminomethane, and mobile phase B is a water solution containing tris-hydroxymethyl aminomethane and sodium chloride. The method can ensure complete separation of supercoiled plasmid DNA, open circle plasmid DNA, linear plasmid DNA and concatemer plasmid DNA in plasmid samples with different molecular weights, has high resolution and high sensitivity, and has important application value in the field of nucleic acid drug analysis technology.