Patents
Literature
81 results about "Biotinylation" patented technology
Efficacy Topic
Property
Owner
Technical Advancement
Application Domain
Technology Topic
Technology Field Word
Patent Country/Region
Patent Type
Patent Status
Application Year
Inventor
In biochemistry, biotinylation is the process of covalently attaching biotin to a protein, nucleic acid or other molecule. Biotinylation is rapid, specific and is unlikely to disturb the natural function of the molecule due to the small size of biotin (MW = 244.31 g/mol). Biotin binds to streptavidin and avidin with an extremely high affinity, fast on-rate, and high specificity, and these interactions are exploited in many areas of biotechnology to isolate biotinylated molecules of interest. Biotin-binding to streptavidin and avidin is resistant to extremes of heat, pH and proteolysis, making capture of biotinylated molecules possible in a wide variety of environments. Also, multiple biotin molecules can be conjugated to a protein of interest, which allows binding of multiple streptavidin, avidin or neutravidin protein molecules and increases the sensitivity of detection of the protein of interest. There is a large number of biotinylation reagents available that exploit the wide range of possible labelling methods. Due to the strong affinity between biotin and streptavidin, the purification of biotinylated proteins has been a widely used approach to identify protein-protein interactions and post-translational events such as ubiquitylation in molecular biology.
Kit for detecting multiple cytokines in tumor immunity as well as preparation method and application of kit
The invention belongs to the technical field of biological detection, and provides a multiple cell factor detection kit in tumor immunity and a preparation method and application thereof, and the detection kit comprises a capture antibody, a detection antibody, a mixed protein standard substance, a diluent and a washing buffer solution. The capture antibody is prepared by coupling a biotinylated antibody with a fluorescent microsphere coated with biotin to obtain a capture antibody coupled with a fluorescent encoding microsphere, and the detection antibody is prepared by coupling derivatized phycoerythrin PE-SMCC with a specific thioether bond of a sulfhydrylated antibody to obtain a fluorescent labeled antibody; the mixed protein standard substance adopts a freeze-drying protection system containing BSA, trehalose and the like. The single hole of the kit can synchronously detect various cell factors, the sample dosage is only 25 microliters, the kit is suitable for immune monitoring of iron overload and other chronic inflammation models, compared with a traditional ELISA method, the efficiency is improved by 10 times, the cost is reduced, and the kit has the remarkable advantages of being high in throughput, wide in linear range and high in stability.
Owner:WUHAN SAIXIAOMAN BIOTECHNOLOGY CO LTD XIANNING BRANCH
Kit for detecting multiple cytokines in macrophage polarization as well as preparation method and application of kit
The invention belongs to the technical field of biological detection, and provides a multiple cell factor detection kit in macrophage polarization and a preparation method and application thereof, and the detection kit comprises a capture antibody, a detection antibody, a mixed protein standard substance, a diluent and a washing buffer solution. The capture system is used for preparing a capture antibody coupled with a fluorescence coding microsphere by coupling a biotinylated antibody with a fluorescence microsphere coated with biotin, and the detection system is used for preparing a fluorescence labeled antibody by coupling derivatized phycoerythrin PE-SMCC with a specific thioether bond of a sulfhydrylated antibody. The mixed protein standard substance adopts a freeze-drying protection system containing BSA, trehalose and the like. The single hole of the kit can synchronously detect multiple cell factors, the sample dosage is only 25 microliters, the kit is suitable for in-vitro tests of mouse mononuclear macrophage leukemia cell lines RAW264.7, compared with a traditional ELISA method, the efficiency is improved by 10 times, the cost is reduced, and the kit has the remarkable advantages of being high in throughput, wide in linear range and high in stability.
Owner:WUHAN SAIXIAOMAN BIOTECHNOLOGY CO LTD XIANNING BRANCH
Method for detecting hydrogen peroxide by utilizing self-biotinylation of guanine quadruplex
PCT designated stageWO2026043246A1Microbiological testing/measurementBiological testingGuanine-QuadruplexesHemin
The present invention relates to a composition, a kit and a detection method for detecting hydrogen peroxide on the basis of self-biotinylation according to the peroxidation activity of a guanine quadruplex, and, more specifically, to a composition, a kit and a detection method for detecting hydrogen peroxide, the composition comprising: i) a guanine quadruplex-hemin complex (G-quadruplex-hemin complex); ii) biotinyl tyramide; and iii) a probe capable of binding to the guanine quadruplex.
Owner:KOREA ADVANCED INST OF SCI & TECH
A single-line-state oxygen self-circulation aggregation-induced emission homogeneous microsphere and a preparation method and a no-washing detection method thereof
This invention discloses a homogeneous microsphere based on singlet oxygen self-circulating aggregation-induced emission, its preparation, and a wash-free detection method, which improves the sensitivity and efficiency of wash-free luminescent oxygen channel immunoassay. This invention successfully prepared AIE receptor microspheres by embedding the photosensitizer AIER dye, ultra-bright AIEG dye, and singlet oxygen-responsive dye SO into carboxyl-modified polystyrene nanoparticles. Simultaneously, an antibody-1-labeled AIE receptor microsphere solution, an antigen-containing test solution, and biotinylated antibody-2 were added to a 96-well plate, followed by streptavidin-modified donor microspheres. After wash-free incubation, the fluorescence signal value was measured, and the concentration of antigen in the test solution was obtained according to a standard curve. This platform completes wash-free, highly sensitive detection within 5 minutes, innovatively combining the aggregation-induced emission characteristics of AIEgens and a singlet oxygen cycling strategy, providing an efficient and universal solution for wash-free diagnostics.
Owner:SOUTH CHINA UNIV OF TECH
Crosslinked streptavidin chromatographic materials and methods of use thereof
Disclosed herein are chromatographic materials comprising crosslinked streptavidin molecules. The crosslinked streptavidin chromatographic materials may be used to prepare affinity chromatographic materials using biotinylated affinity agents, such as a biotinylated antibody, biotinylated antigen-binding fragment, or biotinylated oligonucleotide. Due to the crosslinked nature of the streptavidin molecules, the materials provided herein have reduced to no streptavidin leachate, resulting in improved performance.
Owner:WATERS TECHNOLOGY CORP
Microorganism bioluminescence detection method and kit
PendingCN122038538AMicrobiological testing/measurementChemiluminescene/bioluminescenceA-DNAStreptavidin
The invention belongs to the technical field of microbial nucleic acid detection and bioluminescence analysis, and discloses a bioluminescence nucleic acid detection method and kit based on DNA-RNA hybrid specific recognition. The kit comprises a streptavidin coated solid phase carrier, a biotinylated DNA capture probe, a signal enhancement DNA, a luminescence report fusion protein and a luminescence substrate thereof, the luminescent reporter fusion protein comprises a DNA-RNA hybrid binding domain and a luminescent enzyme domain. According to the method, target RNA is captured through a solid phase, a DNA-RNA hybrid is formed, the luminous report fusion protein is specifically combined with the DNA-RNA hybrid, a bioluminescence signal is generated after a luminous substrate is added, and the target RNA is detected. In the embodiment, an amplification step is not adopted, so that the method has the advantages of simplicity and convenience in operation, rapidness in detection, lower background and better specificity, and can be used for rapid detection of microorganisms.
Owner:SICHUAN UNIV
Method for efficiently preparing fixed-point biotinylation recombinant protein in escherichia coli body
The invention discloses a method for efficiently preparing fixed-point biotinylation recombinant protein in escherichia coli, and belongs to the technical field of biology. According to the invention, a weakened expression regulatory sequence regulates and encodes a biotin ligase (BirA) gene expression cassette and a target protein gene expression cassette encoding a sequence carrying a biotin receptor peptide tag (AVI-Tag) to be jointly constructed on an escherichia coli expression vector, so that efficient fixed-point biotinylation of a target protein in an escherichia coli body is realized. The method has the advantages of high biotinylation efficiency, high specificity, simplicity and convenience in operation and the like, can be widely applied to the fields of separation and purification of proteins, protein interaction research and the like, and has important practical application value.
Owner:DALIAN UNIV
Ribozyme for labeling biotin on target RNA and screening method and application thereof
The invention relates to ribozyme for labeling biotin on target RNA (Ribonucleic Acid) as well as a screening method and application thereof. The RNA labeling technology is one of core tools for modern molecular biology research and clinical diagnosis. A specific marker is covalently linked to a target RNA molecule, so that accurate tracking, efficient detection and functional analysis of RNA can be realized. A biotin-streptavidin system becomes one of the technical routes which are most widely applied due to extremely high affinity and signal amplification capability of the biotin-streptavidin system. At present, common biotin-labeled RNA technical methods mainly comprise a chemical solid-phase synthesis method, an enzymatic method and a chemical labeling method. However, the methods have the limitations of poor site specificity, low efficiency, complex manipulation and the like in the use process. The engineering ribozyme for catalyzing RNA biotinylation is obtained by taking 12: 0 biotin coenzyme A as a biotin donor through an in-vitro screening technology. The ribozyme provided by the invention can efficiently and specifically carry out biotin labeling on target RNA, the reaction process is simple, and the labeling activity is up to 90% or above.
Owner:ZHEJIANG UNIV
Engineered bispecific exosome preparation with tumor targeted breakthrough capability as well as preparation method and application of engineered bispecific exosome preparation
The invention discloses an engineered exosome preparation with tumor targeting and specific gripper response release functions as well as a preparation method and application of the engineered exosome preparation. The preparation comprises a myocardial cell exosome, an NK cell biotinylation activation antibody, a tumor disease biotinylation antibody, streptavidin, a tumor fiber matrix FAP targeting peptide and a tumor matrix enzyme response peptide, and anchoring is carried out through an acetamide compound-polyethylene glycol anchor point platform. The engineering bispecific exosome preparation with good tumor targeting and barrier breakthrough ability and immune cell activation effect is prepared, and the preparation breaks a solid tumor compact fiber barrier and increases NK cell infiltration by utilizing the fibrosis treatment ability of the myocardial cell exosome and the capture and targeting effects of an anchor point platform; the natural killer cells are in close contact with tumor cells, the lasting tumor cell killing effect is achieved, and the tumor matrix environment is fundamentally changed.
Owner:CHINA PHARM UNIV
Multiple lateral chromatography biological detection system and method based on oligonucleotide functionalized gold nanoparticles
According to the oligonucleotide functionalized gold nanoparticle-based multiple lateral chromatography biological detection system and method provided by the invention, high-sensitivity and programmable nucleic acid target detection is realized through functionalized combination of gold nanoparticles and multiple oligonucleotide probes. The system of the invention comprises: 1) a tunable oligonucleotide-AuNP conjugate (each AuNP is linked to 2-3 thiolated probes); 2) a standardized test strip (containing a streptavidin detection line and an ACTB-828 quality inspection line); and 3) a sandwich hybridization mechanism (the target nucleic acid is combined with the AuNP probe and the biotinylation report oligonucleotide at the same time). The method has the beneficial effects that (1) due to the target-induced plasma aggregation effect, an optical signal is enhanced through AuNP close-range aggregation; and (2) a general test paper structure can adapt to a new target only by adjusting a probe sequence. Experiments show that the detection limit reaches 50 pM, and the room temperature stability is gt; the method is suitable for the fields of rapid pathogen detection, on-site diagnosis and the like.
Owner:INDICATE BIOTECHNOLOGY (SHANGHAI) CO LTD
Rapid detection method for cadmium residual quantity in aquatic product based on magnetic qualitative separation and polarized light distinguishing and quantifying
PendingCN121856539AStrong specificityhigh sensitivityPolarisation-affecting propertiesPreparing sample for investigationMicrosphereBiology
The invention relates to a rapid detection method for cadmium residual quantity in aquatic products based on magnetic qualitative separation and polarized light distinguishing and quantifying. The method comprises the following steps: coupling a biotinylated cadmium monoclonal antibody through streptavidin magnetic microspheres, and specifically enriching trace cadmium in the aquatic product digestive juice to form a magnetic compound; a muller matrix microscope is used for collecting a polarization microscopic image of a compound, and a deep learning model (such as RESNET34) is combined to carry out binary classification and particle counting on the image, so that rapid and high-sensitivity quantitative detection of cadmium can be realized. The method is easy and convenient to operate, the target object magnetic separation time is within 2 min, the particle distinguishing accuracy rate is higher than 84%, the quantitative correlation Rreaches 0.9 or above, and the method is suitable for rapid monitoring of trace cadmium (several micrograms / L) in aquatic products.
Owner:FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
Streptavidin chromatographic materials and methods of use thereof
Disclosed herein are chromatographic materials comprising streptavidin molecules, wherein the streptavidin molecules are bound to biotin and one or more biotinylated affinity agents. The biotinylated affinity agent may be a biotinylated antibody, biotinylated antigen-binding fragment, or biotinylated oligonucleotide. The streptavidin chromatographic materials may be used to prepare affinity chromatographic materials. Due to biotin binding to the conjugated streptavidin, the materials provided herein have reduced to no streptavidin leachate, resulting in improved performance.
Owner:WATERS TECHNOLOGY CORP
Cell Selection Chamber And Methods Of Cell Selection
PendingUS20260053997A1Other blood circulation devicesMedical devicesCell selectionMechanical engineering
A cell selection chamber includes an inlet and an outlet, with a reservoir positioned therebetween and configured to allow for fluid flow from the inlet to the outlet. The reservoir contains a plurality of microbubbles, which are each at least partially coated with streptavidin. An upstream membrane is positioned between the inlet and the reservoir, while a downstream membrane is positioned between the outlet and the reservoir. The membranes include a plurality of pores each having a diameter that is less than a diameter of the microbubbles. In use, a heterogeneous population of cells is mixed with a biotinylated antibody additive and then conveyed into the cell selection chamber so as to cause target cells of the heterogeneous population of cells to become bound to the microbubbles and to cause other cells of the heterogeneous population of cells to flow out of the cell selection chamber as unbound cells.
Owner:FENWAL INC
Mutant gene enrichment method, detection method and kit based on Cas9 specific cleavage and isonucleotide magnetic beads
The invention discloses a mutant gene enrichment method, a mutant gene detection method and a mutant gene detection kit based on Cas9 specific cleavage and isonucleotide magnetic beads. The method comprises the following steps: firstly, extracting free DNA; then identifying and cutting a completely matched wild type sequence by using Cas9 protein and specific sgRNA, and reserving the mutant DNA due to base mismatch; cas9 treated DNA and streptavidin magnetic beads containing a biotinylated oligonucleotide probe are incubated, guanine and cytosine in the probe are substituted by isoguanine and isocytosine respectively, and adenine and thymine are modified by locked nucleic acid, so that high-stability homodromous pairing is realized, and mutation DNA is selectively enriched; and finally, carrying out qPCR or sequencing detection on the enriched DNA. The kit has the characteristics of simplicity and convenience in operation, high specificity and high sensitivity, can be used for detecting single-base-level mutation, and is suitable for liquid biopsy, tumor early screening and genetic disease mutation detection.
Owner:SUZHOU HAIMIAO BIOTECH CO LTD
Mycobacterium tuberculosis detection method and kit
PendingCN121496079AMicrobiological testing/measurementMicroorganism based processesMagnetic beadStreptavidin
The invention belongs to the technical field of biology, and provides a mycobacterium tuberculosis detection method and a kit, the detection method comprises the following steps: adding a to-be-detected sample containing mycobacterium tuberculosis into a lysis working solution, carrying out a heat preservation reaction, centrifuging, and taking a supernatant, the cracking working solution contains a biotinylated metal complexing agent and biotinylated nanoparticles, and guanidine isothiocyanate and hydrophobic alkyl groups are connected to the biotinylated nanoparticles; adding a magnetic bead suspension into the supernatant, stirring at room temperature for reaction, standing, taking an upper solution, adding the upper solution into a purification column, purifying and washing to obtain a detection sample, and the surface of a magnetic bead contains streptavidin; and adding a detection sample into an amplification reagent for fluorescent PCR amplification, and analyzing a PCR amplification product by using a fluorescence melting curve to finish detection. The method can effectively reduce impurities contained in the detection sample and improve the detection accuracy.
Owner:ZHUHAI ENCODE MEDICAL ENG
Polar aprotic organic solvent for dissociating streptavidin-biotin conjugate as well as screening method and application of polar aprotic organic solvent
The invention relates to a polar aprotic organic solvent for dissociating a streptavidin-biotin conjugate as well as a screening method and application of the polar aprotic organic solvent. Aiming at the problems that a streptavidin-biotin conjugate system is difficult to elute quickly under a mild condition and a carrier is difficult to regenerate, a solvent molecule descriptor database is constructed; a polar aprotic organic solvent which meets specific molar refractive index and polarity parameters and is a non-hydrogen bond donor is screened from a large number of candidate solvents by utilizing a machine learning model. The solvent can realize rapid reversible dissociation of biotin conjugates from streptavidin at room temperature, and the carrier still keeps high activity after multiple rounds of binding-elution cycles. The solvent is further used for purification of biotinylated small molecules in a crude reaction solution, enrichment and elution of mitochondria and cell surface samples and the like, topological resolution proteomics analysis is achieved, a high-purity product can be obtained in a high-flux mode, and site level topological analysis can be completed.
Owner:SHANGHAI JIAOTONG UNIV
PRRSV (porcine reproductive and respiratory syndrome virus) N protein monoclonal antibody as well as preparation method and application thereof
PendingCN121698996ASsRNA viruses positive-senseAntibody mimetics/scaffoldsPorcine reproductive and respiratory syndrome virusBiotin
The invention discloses a PRRSV (Porcine Reproductive and Respiratory Syndrome Virus) N protein monoclonal antibody as well as a preparation method and application thereof, belongs to the technical field of biological materials, and solves the problem that in the prior art, the stability and specificity of a PRRSV monoclonal antibody screening result are not ideal enough. The method comprises the following steps: constructing a gene of a PRRSV N protein into a plasmid, and performing expression and purification to obtain a recombinant N protein; uniformly mixing the recombinant N protein, a Modifer reagent, a Quencher reagent and a DR-B reagent, centrifuging, taking supernate, re-suspending the supernate in an SB reagent, and separating out biotinylated N protein through a membrane; and screening the positive B cells combined with the biotinylated N protein by using a Hypercell high-throughput single cell screening platform to obtain the monoclonal antibody. The monoclonal antibody aiming at the PRRSV N protein is prepared by a single B cell screening technology, and a foundation is laid for PRRSV diagnosis and related research of N protein functions.
Owner:JINLIN MEDICAL COLLEGE
Reagents for detecting varicella-zoster virus infection and methods of making same
ActiveCN116539875BVirus peptidesNucleic acid vectorVaricella-zoster virus infectionSerum protein albumin
The present application relates to the field of immunological detection, and particularly relates to a reagent for detecting varicella-zoster virus infection and a preparation method thereof. The reagent comprises R1 reagent and R2 reagent; the R1 reagent is a working solution of streptavidin magnetic beads, and the preparation method comprises the following steps: firstly coupling activated carboxyl magnetic beads with streptavidin; dialyzing the magnetic beads obtained by the first coupling to remove streptavidin physically combined with the magnetic beads; secondly coupling the dialyzed magnetic beads with streptavidin; quenching the magnetic beads obtained by the second coupling, and then placing the magnetic beads in a working solution to obtain the working solution; the working solution contains fetal bovine serum, bovine serum albumin and lysine; the R2 reagent is a biotinylated VZV gE recombinant protein, and the VZV gE recombinant protein is a varicella-zoster virus gE glycoprotein extracellular domain fragment expressed by human embryonic kidney cells. The reagent has high sensitivity and specificity in the detection of varicella-zoster virus infection.
Owner:PEKING UNIV
A method and a kit for generating nucleic acid for target capture
The invention relates to a method and a kit for generating nucleic acid for target capture. The method involves binding of a circ-olio sequence to at least one target oligonucleotide to generate a single stranded circular DNA. The single stranded circular DNA is bidirectionally amplified with the sense and antisense oligonucleotides, followed by biotinylation to obtain for a double-stranded concatemer of linear sequence. The double-stranded concatemer of linear sequence with the restriction endonucleases produces a target capture nucleic acid. The method offers a scalable targeted capture of pr eselected genomic regions, and is cost effective. The method of generating nucleic acid for target capture has application in the field of oncology hematology, inherited malignancies, germline disorders, microbiology and prenatal diagnosis. The invention generates baits over high GC-rich regions of the genome.
Owner:TATA MEMORIAL CENTRE-ADVANCED CENTRE FOR TREATMENT RESEARCH & EDUCATION IN CANCER (TMC-ACTREC)
Anti-CD4 antibody and microsphere flow-type immunofluorescence luminescence detection kit and application thereof
PendingCN121699012AImmunoglobulins against cell receptors/antigens/surface-determinantsBiological testingAutoimmune conditionBinding site
The invention provides an anti-CD4 antibody as well as a microsphere flow immunofluorescence luminescence detection kit and application thereof, and provides two monoclonal antibodies for recognizing different binding sites of a CD4 antigen, an antibody coupling system based on magnetic fluorescent microspheres, a biotinylation detection antibody and phycoerythrin labeled streptavidin. The invention also discloses a corresponding detection method and a preparation method of the kit. The CD4 antigen detection kit and the antibody combination thereof provided by the invention can significantly improve the sensitivity, stability, specificity and flux capacity of CD4 detection, and are suitable for multiple fields of immune function evaluation, autoimmune disease evaluation, inflammatory disease research, HIV infection auxiliary diagnosis and the like.
Owner:THE STOMATOLOGIAL HOSPITAL OF ZHEJIANG UNIV SCHOOL OF MEDICINE
A detection method and kit for detecting anti-nephrin antibodies
The present application relates to the technical field of protein detection, and particularly relates to a detection method and kit for detecting anti-Nephrin antibody. The detection method comprises the following steps: using biotinylated Nephrin protein to capture anti-Nephrin antibody in a sample to be detected on a SA matrix to obtain a first complex; performing acidolysis desorption, neutralization and Protein G directed antibody immobilization on the first complex to obtain a second complex; using a biotin-streptavidin-HRP system to detect the second complex; the acidolysis desorption comprises the following steps: using an acid solution with a pH of 2-3 to treat the first complex, and treating at 35-40 DEG C and 500-800 rpm for 10-20 minutes. The method provided by the present application can accurately and quickly detect the anti-Nephrin antibody level in a sample, and has important application value.
Owner:THE FIRST MEDICAL CENT CHINESE PLA GENERAL HOSPITAL
Antigen chromatography columns and methods of use thereof
PCT designated stageWO2026093919A1Other chemical processesSolid sorbent liquid separationAntigenBinding site
Disclosed herein are antigen chromatography columns. Said antigen chromatography columns utilize streptavidin-functionalized particles, wherein the streptavidin binding sites are occupied with one or more biotinylated antigens. In this way, said antigen chromatography columns may be used to isolate, purify, and characterize antibodies (or other proteins) that specifically bind to said antigens.
Owner:WATERS TECHNOLOGY CORP
Kit for detecting plasma IgG glycosylation level and detection method thereof
The invention discloses a kit for detecting plasma IgG glycosylation level and a detection method thereof, and relates to the technical field of biological detection.Plasma IgG is specifically captured through protein A, four biotinylation lectins are combined, sialylation, galactosylation, fucosylation and mannosylation levels of IgG can be synchronously and quantitatively detected, a standardized ELISA system is established, and the kit is used for detecting the plasma IgG glycosylation level. Operation is easy and convenient, repeatability is good, and the problem that multi-dimensional simultaneous detection cannot be achieved in the prior art is solved. The kit is low in cost, does not need complex instruments, is suitable for large-scale clinical popularization, and fills the market blank of commercial IgG glycosylation ELISA kits. Clinical samples of colorectal cancer prove that patients and healthy people can be effectively distinguished, in-vitro risk assessment can be realized by combining a column diagram model, the detection efficiency is excellent, and the method can be expanded to be applied to assessment and monitoring of various tumor and inflammation related diseases, and has high clinical value.
Owner:THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
High sensitivity biotinylated peptide binding elisa assay
Immunoglobulin light Chain "AL" amyloidosis is the most common form of systemic amyloidosis, accounting for approximately 70% of the diagnosed cases in developed countries. As there are treatments available, e.g., based on antibody C11-1F4, and in development for AL amyloidosis, there is a need in for more accurate methods for quality control of c11-1F4 antibodies or antigen-binding fragment thereof. Immunoassays, methods, and kits for testing the specificity of 11-1F4 antibodies are provided. The immunoassays, methods, and kits are more than 10 times more sensitive than the ELISA protocol that is routinely used for evaluation of c11-1F4 binding to LEN peptide.
Owner:ALEXION PHARMACEUTICALS INC
High-quality single-cell Hi-C library building method
The invention relates to the technical field of single-cell sequencing, and discloses a high-quality single-cell Hi-C library building method, which comprises the following steps: by utilizing a cumulative effect, carrying out cross-linking immobilization, enzyme digestion, biotinylated nucleotide filling-in and connection on population cells, sorting single cells, immediately marking different bar code combinations on each cell, mixing extracts of each cell, and carrying out high-quality single-cell Hi-C library building. After mixing, the cell population is equivalent to a small amount of cell population, enriching biotinylated DNA by using streptavidin magnetic beads, and finally establishing a library. According to the method, the library quality can be remarkably improved while the single cell resolution is maintained.
Owner:THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
Detection reagent, kit and application thereof in detection of tumor markers
The invention relates to the technical field of tumor diagnosis, in particular to a detection reagent, a kit and application of the detection reagent to detection of tumor markers. The sMIC quantitative detection kit mainly comprises a magnetic separation reagent, an enzyme reactant, a substrate solution, a calibration product and a quality control product, and further comprises a diluent, a buffer solution and a cleaning solution. The magnetic separation reagent is a streptavidin magnetic microsphere coupled biotinylated anti-MICA antibody, the enzyme reactant is an acridinium ester labeled anti-MICA / B antibody, the substrate solution is an enzymatic chemiluminescence substrate solution, and the calibration product and the quality control product are solutions containing recombinant MICA / B protein. According to the reagent and the kit provided by the invention, by optimizing a coupling process and reaction system design of the magnetic beads and the antibodies, the coupling efficiency of the magnetic beads and the antibodies is improved, and the detection sensitivity and the anti-interference capability are remarkably improved.
Owner:GUANGZHOU KONCEN BIOSCI
Acidithiobacillus ferrooxidans immobilized material and application thereof in cell immobilization and desulfurization
The invention belongs to the technical field of cell immobilization, and particularly relates to an acidophilic thiobacillus ferrooxidans immobilization material and application thereof in cell immobilization and desulfurization. According to the acidithiobacillus ferrooxidans immobilized material, Fe3O4 nano-clusters are used as raw materials, a PDA shell layer with a mesoporous structure is formed on the surfaces of the Fe3O4 nano-clusters by means of self-polymerization reaction of dopamine, a nano composite material Fe3O4 (at) mPDA is constructed, meanwhile, biotinylated chitosan derivatives Bio-CS are synthesized by using biotin as raw materials, and the acidithiobacillus ferrooxidans immobilized material is prepared. And combining the Fe3O4 (at) mPDA with a biotinylated chitosan derivative Bio-CS by virtue of an emulsification method, thereby obtaining the Fe3O4 (at) mPDA. The acidithiobacillus ferrooxidans immobilized material provided by the invention can be used for immobilizing acidithiobacillus ferrooxidans. The material provided by the invention not only can effectively maintain the biological activity of the acidithiobacillus ferrooxidans BY3, but also has the advantages of mild reaction conditions, no secondary pollution and capability of maintaining the cyclic utilization of Fe < 3 + >, so that the method meets the manufacturing requirements of a green biotechnology.
Owner:XINXIANG MEDICAL UNIV
Anti-ADGRG1 antibody and microsphere flow-type immunofluorescence luminescence detection kit and application thereof
PendingCN121699011AImmunoglobulins against cell receptors/antigens/surface-determinantsBiological testingAutoimmune conditionBinding site
The invention provides an anti-ADGRG1 antibody as well as a microsphere flow type immunofluorescence luminescence detection kit and application thereof, and provides two monoclonal antibodies for recognizing different binding sites of an ADGRG1 antigen, an antibody coupling system based on magnetic fluorescent microspheres, a biotinylation detection antibody and phycoerythrin labeled streptavidin. The invention also discloses a corresponding detection method and a preparation method of the kit. The ADGRG1 antigen detection kit and the antibody combination thereof provided by the invention can significantly improve the sensitivity, stability, specificity and flux capacity of ADGRG1 detection, and are suitable for multiple fields of immune function evaluation, autoimmune disease evaluation, inflammatory disease research, HIV infection auxiliary diagnosis and the like.
Owner:THE STOMATOLOGIAL HOSPITAL OF ZHEJIANG UNIV SCHOOL OF MEDICINE
Kits for biotin-streptavidin pulldown system and method
The present disclosure provides a method for isolating a biotinylated molecule from a biological sample, using an affinity ligand conjugated with a first nucleotides and a solid support conjugated with a second nucleotides, wherein the affinity ligand specifically binds the biotinylated molecule to form a complex, the first nucleotides is complementary to the second nucleotides. As a result, the complex can be captured by the solid support through the hybridization between the first oligonucleotide and the second oligonucleotide. The complex may subsequently be released from the solid support by applying heat equal to or above a melting temperature (Tm) sufficient to disrupt the hybridization. The present disclosure also provides a kit useful for performing the method described herein.
Owner:SYNCELL (TAIWAN) INC
Method for diagnosing peanut allergy based on hydrogen peroxide sensitive quantum dots
The invention relates to a method for detecting peanuts based on catalase (CAT) mediated hydrogen peroxide sensitive CdTe QDs fluorescent competition ELISA (enzyme-linked immuno sorbent assay). At present, hydrogen peroxide sensitive QDs are not applied to detection of food allergens, so that the method comprises the following steps: 1) affinity purification of an antibody; 2) carrying out biotinylation on IgG; (3) carrying out a common sELISA program; (4) characterizing the CdTe QDs (Quantum Dots); (5) preparing a streptavidin-catalase conjugate; the invention discloses a method for detecting peanuts in food, which comprises the following steps of (1) preparing a sample, (2) preparing an SA-CAT conjugate, (3) preparing an SA-CAT conjugate, (4) representing the SA-CAT conjugate, (7) optimizing conditions based on a fluorescence quenching sELISA method, (8) analyzing the detection performance of the fluorescence sELISA method, (9) detecting a sample, (10) carrying out Tricine-SDS-PAGE electrophoresis and (11) carrying out data statistics and analyse.The peanut detection method established by the invention has the advantages of being good in specificity, high in sensitivity and the like, can be used for detecting the peanuts and sensitization residues thereof in the food, and has good popularization and application prospects.
Owner:邱毓