403results about How to "Easy separation" patented technology

[Problems]To provide a membrane material that realizes effective and efficient separation of a target substance of micron size, being easy to handle and that can be worked into various forms; a blood filtration membrane and a leukocyte removing filter unit that realizes a substantial reduction of filter material volume while retaining high capability of removing leukocytes, thereby reducing the loss of hemocyte suspension; and a cell culturing diaphragm suitable for co-culturing and a relevant method of cell culturing. [Means for Solving Problems]There is provided a composite porous membrane comprising a porous membrane comprised of an organic polymeric compound, and a supporting porous membrane adjacent to the porous membrane, characterized in that the organic polymeric compound constituting the porous membrane penetrates in at least part of a surface adjacent to porous membrane of the supporting porous membrane, the porous membrane having specified opening ratio, average pore diameter, standard deviation of pore diameter, ratio of through pore, average membrane thickness, standard deviation of membrane thickness and internal structure, and that the supporting porous membrane has communicating pores of 0.5D μm or greater average pore diameter. Further, there are provided a blood filtration membrane comprising the composite porous membrane; a leukocyte removing filter unit comprising the composite porous membrane as a second filter; and, utilizing the composite porous membrane, a cell culturing diaphragm and method of cell culturing.

The invention provides a production method for extracting sweet tea glucoside from sweet tea leaves. The method comprises the following process flows of: smashing a sweet tea crude drug; extracting a solvent; concentrating; precipitating with alcohol; purifying with macroporous resin; concentrating; carrying out chromatography with polyamide resin; concentrating; crystallizing and recrystallizing; drying; and obtaining a finished product. By using the method, a high-quality sweet tea glucoside product can be produced, and the method also has the advantages of reducing the material loss, shortening the production period, improving the product yield and reducing the production cost and is suitable for the industrial production; the purity of the sweet tea glucoside produced by utilizing the method is higher than 98 percent, so that the product purity is high, and the component is single; the color of the sweet tea glucoside is white and good; and meanwhile, the technical difficulty of industrially producing a high-content and high-quality sweet tea glucoside extractive is overcome, so that large-scale production is smoothly produced.

The invention discloses a preparation method of metformin hydrochloride. The method comprises: taking 40% dimethylamine as a starting material, which is subjected to a salification reaction with hydrochloric acid salt forming reaction of dimethylamine hydrochloride, subjecting the dimethylamine hydrochloride and dicyandiamide to an addition reaction in solvent N, N-dimethylformamide so as to generate a metformin hydrochloride crude product, then conducting refining, thus obtaining metformin hydrochloride. The solvent of the invention has viscosity insusceptible to temperature, and is easy to be separated from the product. Besides, the solvent can dissolve the reactant dicyandiamide, and even if dicyandiamide is not fully reacted, it cannot be separated out during separation of the product and the solvent, so that the production operation can be easier, and the purity of the final product can be very high. The method of the invention has the advantages of simple and easy production operation, high raw materialutilization rate, and cheap as well as easily available solvent, the market price of which is 2-3 times lower than prices of solvents in prior, and the synthesis cost is reduced by about 30%, so that the preparation method of the invention is very suitable for industrial production of metformin hydrochloride.

The invention relates to a preparation method of copper, cobalt and sulfur micropowder. The copper, cobalt and sulfur micropowder is copper, cobalt and sulfur ternary composite sulfide CuCo2S4 solid powder, the color is black brown or black, the shape is amorphous or in cubic crystalline phase, the powder dimension is 10<-5> to 10<-8>m; the preparation method is as follows: divalent copper salt, divalent cobalt salt and an organic sulfur compound are synthesized by a one-step hydrothermal method. The preparation method is simple and is easy to realize industrial production, the prepared copper, cobalt and sulfur micropowder has excellent electrochemical properties, higher specific capacity and good cycle stability.

This invention relates to a method of using hydrosilylation reaction to synthesize silicon compound. It needs to solve the technical problem that provides a hydrosilylation reaction method taking ionic liquids of room temperature as the reaction medium and transition metal complexes Rh (PPh3) 3Cl as the main catalyst. The greatest strength is that it can improve the catalytic activity of Rh (PPh3) 3Cl and increase selectivity of beta adduct in the ionic liquid at room temperature. At the same time, when the reaction ends, cooling and settling make the catalyst/ionic liquid and products separate, and the catalyst/ionic liquid can be recycled.

The invention relates to a method for separating and extracting cholesterol in lanolin alcohol, which comprises the following steps: carrying out molecular distillation on lanolin alcohol, collecting the light-phase fraction which is refined lanolin alcohol, heating the refined lanolin alcohol in a mixed solvent of methanol and acetone until the refined lanolin alcohol is completely dissolved, and cooling to cooling temperature, wherein the remainder of the filtrate after reduced pressure distillation is the primary concentrate of cholesterol; heating to dissolve the primary concentrate of cholesterol in acetone, cooling to precipitate, filtering, and carrying out reduced pressure distillation on the filtrate to recover the solvent, wherein the balance is the secondary concentrate of cholesterol; heating to dissolve the secondary concentrate of cholesterol in an alcohol solvent, cooling to cooling temperature, keeping the temperature for 6-12 hours, and vacuum-filtering to obtain the white acerose cholesterol crude product; and recrystallizing the cholesterol crude product through a methanol-acetone mixed solvent to obtain the refined cholesterol product. The refined cholesterol product selectively recrystallizes through the solvent to obtain the byproduct lanosterol accounting for 63-70% and the cholesterol product of which the purity is more than 90%.

The invention relates to a method for preparing kaempferol. The method comprises the following technical steps of: crushing raw materials, and extracting alkaline solution; performing enzymolysis after acid adjustment, and extracting enzymolysis liquid by using ethyl acetate; and performing concentration under reduced pressure on the extract liquor, and recrystallizing by using methanol to obtain the product. The method for producing the kaempferol has the advantages of simple process, high utilization ratio of raw materials, and high purity.

The invention discloses a preparation method of rosuvastatin calcium. The method comprises the following steps of: synthesizing an important intermediate, i.e., [4-(4-fluorophenyl)-6-isopropyl-2-(N-methyl-N-mesyl amino)pyrimidine-5-radical]methanol (compound IV); and preparing the rosuvastatin calcium (compound I). The method has the advantages of readily available raw materials, high stereoselectivity, high yield and suitability for industrial production.

The present invention is the preparation process of composite inorganic flame retardant with nanometer attapulgite clay core and coating of Mg, Al, Sb, etc. The preparation process of the composite inorganic flame retardant includes the following steps: compounding water suspension of nanometer attapulgite clay with solid content of 10-200g/L; heating to 40-100 deg.c and coating the nanometer attapulgite clay with inorganic material Al2O3 hydrate, MgO hydrate, antimony oxide hydrate, ZnO hydrate and/or zinc borate via stirring; letting stand for the coated nanometer attapulgite clay to deposit; solid-liquid separation; filtering, washing, drying at 80-150 deg.c and crushing to obtain the composite inorganic flame retardant. The composite inorganic flame retardant of nanometer attapulgite clay has both flame retarding and excellent reinforcing effects.

Provided is a refrigerator (10). The refrigerator (10) includes a main body (11) having a storage space, a door (14) opening or closing the storage space, a dispenser (20) provided in the door (14) to dispense cooled water and purified water, a purified water input part (251) provided in the dispenser (20) to input a command for dispensing the purified water, a cooled water input part (252) provided in the dispenser (20) to input a command for dispensing the cooled water, a dispensing amount input part (255) for setting an amount of purified water to be dispensed when the purified water is selected through the purified water input part (251), a filter device (40) provided within the storage space to purify water supplied from a water supply source (1) outside the main body (11), a water tank (50) storing the water passing through the filter device (40), the water tank (50) configured to cool the stored water by using cool air within the storage space, a purified water passage (62) guiding the purified water filtered in the filter device (40) into the dispenser (20), a cooled water passage (63) guiding the cooled water cooled in the water tank (50) into the dispenser (20), a purified water valve (622) controlling a water flow in the purified water passage (62), and a cooled water valve (632) disposed in the cooled water passage (63) to control a water flow in the cooled water passage (63). When the purified water is selected through the purified water input part (251), the purified water valve (622) is opened, and the cooled water valve (632) is closed, when an amount of water set through the dispensing amount input part (255) has been dispensed, the purified water valve (622) is closed, and when the cooled water is selected through the cooled water input part (252), the cooled water valve (632) is opened, and the purified water valve (622) is closed.

The invention discloses an immobilized laccase, which adopts magnetic mesoporous carbon as a vector, the laccase is immobilized on the magnetic mesoporous carbon by means of the physical adsorption effect, magnetic nanoparticles are embedded in the pore canals of the magnetic mesoporous carbon, the amount of the laccase adsorpted on the magnetic mesoporous carbon is more than 140mg/g, and the enzyme activity recovery rate of the immobilized laccase is 60 to 95 percent. The invention correspondingly discloses a preparation method for the immobilized laccase, which includes the following steps:the magnetic mesoporous carbon and laccase solution are first prepared, the concentration of the laccase solution is 0.3mg/ml to 1.0mg/ml, and the pH value of the laccase solution is 3.0 to 6.0; the magnetic mesoporous carbon is then added into the laccase solution, and the solution is oscillated under the temperature of 25 DEG C to 30 DEG C for more than three hours; and after subsequent processing, the immobilized laccase is obtained. Since the immobilized laccase disclosed by the invention is immobilized on the basis of the magnetic mesoporous carbon, the laccase has excellent enzymological properties; and the preparation method for the immobilized laccase is easy to operate, and the cost is low.

The invention provides a method for extracting theaflavin and theanine from tea leaves. According to the method, water is added into fresh tea leaves, the tea leaves are ground, the ground tea leaves are dissolved through coarse tea polyphenol addition by adopting ultrafine cladosporium fermentation liquid for replacing water, then, magnesium chloride and copper chloride solution is added, the oxidation is carried out under the acid condition, the stirring is carried out, the ventilation reaction is taken, tea pigment using theaflavin and thearubigins as major ingredients is produced under the condition of enzymatic oxidation polymerization reaction, and then, filtering separation, purification, concentration and drying are carried out to obtain tea pigment and theanine.

The invention relates to a method for extracting low-pesticide-residue ginsenoside from ginsengs. The method comprises the following steps of leaching the ginsengs for 3-4 times in cold water, wherein each leaching lasts for 8-12 hours; combining leaching liquids, carrying out concentration, solvent extraction and concentration, enabling the leaching liquids to pass through a macroporous resin column, and flushing by utilizing water; carrying out ethanol elution, collecting the eluted liquid, and drying to obtain the low-pesticide-residue ginsenoside. The production method of the ginsenoside comprises two purification processes, the preliminary purification is extraction, and the resin is highly purified; partial impurities are removed in the extraction process, so that the high purification efficiency is improved.

The invention belongs to the technical field of preparation of an electronic reagent and relates to a device and a method for preparing electronic ethylene carbonate by coupling crystallization with rectification. The device for preparing electronic ethylene carbonate by coupling crystallization with rectification provided by the invention comprises a raw material storage tank, a falling film crystallizer, a rectifying column, a first product tank, a transition tank and a second product tank, wherein the raw material storage tank is connected with a feeding port of the falling film crystallizer; a discharging head pipe is arranged at a discharging port of the falling film crystallizer; the discharging head pipe is respectively connected with the feeding port of the falling film crystallizer, the first product tank and the transition tank; the transition tank is connected with the rectifying column; an outlet at the top of the rectifying column is connected with the second product tank; the outlet at the bottom of the rectifying column is connected with the raw material storage tank. According to the invention, the two technologies of falling film crystallization and rectification are coupled with each other, the deficiencies thereof are mutually made up, more efficient separating operation is realized, the prepared ethylene carbonate can reach ultra-clean high-purity electronic level, and meanwhile, the structure is simple, the operation is convenient, the energy consumption is low and the cost is low.

Provided is a separation method for a mixture of triethylene-diamine and ethanolamine. In the method, dimethylbenzene or ethylbenzene serves as an entrainer, and the mixture of triethylene-diamine and ethanolamine is separated through heterogeneous azeotropic rectification. The method includes the following steps: 1 the mixture and the entrainer of triethylene-diamine and ethanolamine enter an azeotropic rectifying tower, overhead fraction is an azeotrope of the entrainer and ethanolamine, and tower kettle fraction is triethylene-diamine product; 2 the overhead fraction of the azeotropic rectifying tower enters a phase segregator for phase segregation, the upper layer is the entrainer and circulated to the azeotropic rectifying tower, and the lower layer is ethanolamine crude product and enters an ethanolamine refining tower; and 3 overhead fraction of the ethanolamine refining tower is an azeotrope of the entrainer and ethanolamine and circulated to the azeotropic rectifying tower, and tower kettle fraction is ethanolamine product. The method is suitable for separating the triethylene-diamine and ethanolamine mixture obtained through amination reaction with ethanolamine and liquid ammonia as raw materials.

The invention provides a wet and dry vacuum cleaner comprising a floor brush, an upright machine body, a cleaning liquid supply assembly, a waste water recovery assembly and a portable cleaning device. The floor brush defines a dust collection cavity; the machine body is rotatably connected to the floor brush; the cleaning liquid supply assembly comprises a cleaning liquid tank and a spray head, wherein the cleaning liquid tank is arranged on the machine body and communicates with the spray head and the spray head is suitable for spraying cleaning liquid to the floor brush or a to-be-cleaned surface; the waste water recovery assembly comprises a waste water tank communicating with the dust collection cavity, wherein the waste water tank is arranged on the machine body; the portable cleaning device is arranged on the machine body detachably and comprises a dust drum component and a power component, wherein an air inlet of the dust drum component communicates with the waste water tank; the detaching direction in separation of the portable cleaning device and the machine body is parallel to the extending direction of the machine body or at an acute angle to the extending direction ofthe machine body. The wet and dry vacuum cleaner has the advantages of high flexibility, good cleaning effect, compact structure and convenient use; the separation operation of the portable cleaning device is convenient and easy.

The present invention provides a clarified and concentrated fruit juice preparation method which includes the following steps: fruits are crushed and beat to prepare fruit pulp, the fruit pulp is sent into a freezing and concentrating equipment to conduct suspended crystallization and then conduct gradual layered crystallization until all the fruit pulp is frozen, the fruit flesh and other suspended particles can be effectively blocked in the ice layer with the help of filtering bed constituted by frozen ice particles, and the concentrated juice is separated out and clarified. The preparation method eliminates the necessary processes needed for the production of conventional clarified juice such as juice squeezing, filtrating, clarifying, etc. The preparations of fruit pulp to clarified concentrated juice are all conducted in a single freezing and concentrating device, the preparation occupies less devices, the technology process and operation were simple and convenient, the investment and operating costs are low, and the energy is saved and the consumption is reduced.

The invention relates to the technical field of biological separation and extraction, in particular to an extracting method of extracellular vesicles. The method comprises the steps that a biologicalsample is provided; the biological sample is in contact with a capturing surface at least twice on the condition that impurities in the biological sample are partially or wholly reserved on the capturing surface, wherein the impurities are partial or whole other substances except the extracellular vesicles in the biological sample, and the capturing surface comprises an inner surface and/or outersurface of a spherical-particle porous material; when the impurities are partially or wholly reserved on the surface of a capture, breakthrough liquid is extracted liquid containing the extracellularvesicles; or the breakthrough liquid is further concentrated. By means of the method, the epicyte vesicles can be quickly separated and purified, the separation operation is simple, the single use cost is low, the separated sample in high in purity, the bioactivity of the sample is kept good, and the extracting method is applied to fundamental research in the aspects of biomarker discovery, biotherapy and the like and industrial production.

The invention discloses a method for separating oil-phase dichloropropanol from a mixed aqueous solution of dichloropropanol and chlorine hydride, which comprises the following steps of: mixing a metal halide with the mixed aqueous solution of dichloropropanol and chlorine hydride to obtain a metal halide-contained mixed solution; and carrying out mechanical agitation for 0.5-10 hours under the condition of heating or evaporation or rectification on the metal halide-contained mixed solution, so as to separate the oil-phase dichloropropanol from the mixed solution. According to the method disclosed by the invention, the principle of salt effect is utilized, the azeotropic point of the existing mixed aqueous solution of dichloropropanol and chlorine hydride is broken through adding the metal halide, so that the separation of the mixed aqueous solution of dichloropropanol and chlorine hydride is realized; and further, the metal halide is recycled, and thus, the handling capacity of three wastes is not increased. The investment on the whole device is little, the occupation of land is little, and the operation for separating dichloropropanol is simple.

The invention relates to the technical field of chemical engineering production equipment, in particular to a magnetic material catalyst separating device and system. On the basis of the magnetic adsorption principle, the aim of clearing away magnetic material catalysts mixed in chemical liquid materials can be achieved. According to the device and system, the machining process of chemical engineering products required to use the magnetic material catalysts is improved, the sedimentation time of a sedimentation process is shortened, in the filtering process, a user does not need to filter out the magnetic material catalysts again, work burdens of filtering equipment are reduced, and the production efficiency is improved. When a magnetic rod sleeve pipe array absorbs the magnetic material catalysts in a full-load mode, the aim of separating the magnetic material catalysts can be achieved by taking out the magnetic rod sleeve pipe array from a magnetic material catalyst separating cavity can be achieved. The aim of secondarily recycling magnetic material catalyst particles can be achieved by taking out a permanent magnet magnetic stick or performing power-failure treatment on an electromagnet can be achieved. The better separating effect can be achieved by enabling a plurality of magnetic material catalyst separating devices to be connected in series for use.

The invention discloses a method for producing gamma-amino butyric acid through continuous conversion of an immobilized enzyme. The method comprises the following steps of: inducing and expressing an established glutamic acid decarboxylase gene engineering bacterium BL21 (DE3)-pET35b-GAD; performing cell disruption; collecting supernatant; and researching optimal reaction conditions and immobilizing conditions of a glutamic acid decarboxylase in fusion expression with a cellulose binding domain (CBD). Cellulose is specific adsorbed by a CBD label, and a cellulose carrier is added into cell lysate, so that the specific adsorption of the glutamic acid decarboxylase is realized, and the immobilized glutamate decarboxylase is crosslinked by using a crosslinking agent. The conversion conditions are optimized and the optimal catalytic reaction conditions are as follows: the pH is 5.5; the reaction time is 2 hours; the concentration of a substrate L-sodium glutamate is 40g/ L; the concentration of the immobilized enzyme is 8mL/L; and the concentration of a coenzyme pyridoxal phosphate aldehyde is 0.1mM. Under the optimal reaction, continuous conversion is repeated for 10 times and the conversion rate of the substrate L-sodium glutamate is more than 93%.

The invention relates to a hydrosilylation of rhodium complex catalyzed alkene in room-temperature ion liquid/super-critical CO2 medium. The technical problem to be solved is characterized in that thehydrosilylation condition is moderate and safe, the reaction conversion rate is high, the outcome Beta-addition product of the hydrosilylation has strong selection, no outgrowth paraffin hydrocarbonis produced, the outcome and the catalyst are convenient to be separated from each other, and the catalyst can be reclaimed and re-utilized. The hydrosilylation is characterized in that the alkene andhydrogen-contained silanes are taken as raw materials, rhodium compound is taken as the catalyst, the potassium tert-butoxide is taken as adjuvant, and the room-temperature ion liquid/supercritical CO2 is taken as reaction medium; and the mixture is heated and mixed so as to react sufficiently, the outcome is collected by reducing pressure, thus obtaining the hydrosilylation products.