The invention provides a preparation method of a medical high-transparency collagen solution, which comprises the following steps: S1, taking fresh animal tissues, removing tissues such as hair,
fascia, fat and blood meat,
slicing, cleaning to
remove blood water, washing with water, and freezing and crushing; s2, soaking the crushed materials in a mixed
degreasing agent for
degreasing; the mixed
degreasing agent comprises a cationic surface
active agent and
lipase, and the cationic surface
active agent comprises at least one of a bio-based surface
active agent and a
quaternary ammonium salt type surface active agent; the bio-based surfactant comprises at least one of
rhamnolipid,
sophorolipid and
trehalose lipid; s3, freezing the degreased material, irradiating, soaking in
purified water, filtering, and collecting a swelled material; s4, carrying out enzymolysis on the swelled substance; s5, collecting filtrate after enzymolysis, filtering after
salting out, collecting filter residues, and cleaning in a
salt solution to obtain a
salting-out substance; s6, the
salting-out substance is added into pure water to be dissolved, the
mass fraction of collagen is controlled to range from 0.01% to 6.5%, then
ultrafiltration is conducted, filtrate is collected, low-temperature storage is conducted, and a collagen solution is obtained. The collagen prepared by the preparation method has relatively high transparency, and meanwhile, the structure of the collagen is not damaged in the preparation process, that is, the finally obtained collagen solution still has a complete
triple helix structure. And
heavy metals or toxic and harmful reagents are not added in the whole extraction process, so that the problem of residues of the
heavy metals or the toxic and harmful reagents does not exist, and the application range is widened.