Method for preparing lysosomal collagen

A collagen and soluble technology, which is applied in the field of preparation of enzyme-soluble collagen, can solve the problems of high cost, poor thermal stability of enzyme-soluble collagen, complex preparation process, etc., and achieve low production cost, simple and reasonable method, and high yield. rate effect

Inactive Publication Date: 2011-08-10
HUAIHAI INST OF TECH +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Its disadvantages are: the cost of this method is high, the thermal stability of the

Method used

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  • Method for preparing lysosomal collagen
  • Method for preparing lysosomal collagen
  • Method for preparing lysosomal collagen

Examples

Experimental program
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Effect test

Embodiment 1

[0020] Example 1. A preparation method of enzyme-soluble collagen, the steps are as follows:

[0021] (1) Raw material treatment: use fish skin or other animal tissues as raw materials, wash with water, soak in 0.05mol / L NaOH solution at 0°C for 1 hour, during the soaking process, change the soaking solution every 1 hour, Raw materials for removing miscellaneous proteins; then soaking in detergent or organic solvent for 1 hour, and then washing with water at 0°C to obtain raw materials for removing fat;

[0022] (2) Collagen extraction: add 0.05mol / L acetic acid, citric acid or hydrochloric acid solution to the processed raw materials as extractant for extraction for 2 hours, the extraction temperature is 0°C, and add 0.05% of the weight of raw materials at the same time Protease, the weight ratio of raw material and extractant is 1:5, after leaching and filtering, the obtained filtrate is enzyme-soluble collagen crude extract;

[0023] (3) Collagen purification and inactiva...

Embodiment 2

[0024] Example 2. A preparation method of enzyme-soluble collagen, the steps are as follows:

[0025] (1) Raw material treatment: use fish skin or other animal tissues as raw materials, wash with water, soak in 5.0mol / L NaOH solution at 40°C for 50 hours, during the soaking process, change the soaking solution every 5 hours, Raw materials for removing miscellaneous proteins; then soaked in detergent or organic solvent for 50 hours, and then washed with water at 30°C to remove raw materials for fat;

[0026] (2) Collagen extraction: add 10mol / L acetic acid, citric acid or hydrochloric acid solution to the treated raw materials as extractant for extraction for 72 hours, the extraction temperature is 40°C, and add 10% pepsin by weight of raw materials at the same time , the weight ratio of the raw material to the extractant is 1: 100, filtered after leaching, and the obtained filtrate is the crude enzyme-soluble collagen extract;

[0027] (3) Collagen purification and inactivat...

Embodiment 3

[0028] Example 3. A preparation method of enzyme-soluble collagen, the steps are as follows:

[0029] (1) Raw material treatment: use fish skin or other animal tissues as raw materials, wash with water, soak in 0.5mol / L NaOH solution at 4°C for 20 hours, during the soaking process, change the soaking solution every 3 hours, The raw material for removing miscellaneous protein; then soak in detergent or organic solvent for 20 hours, and then wash with water at 2°C to get the raw material for removing fat;

[0030] (2) Collagen extraction: add 0.1mol / L acetic acid, citric acid or hydrochloric acid solution to the processed raw materials as extractant for extraction for 48 hours, the extraction temperature is 3°C, and add 0.5% of raw material weight stomach Protease, the weight ratio of raw material and extractant is 1:20, after leaching and filtering, the filtrate obtained is the crude enzyme-soluble collagen extract;

[0031] (3) Collagen purification and inactivation of enzym...

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Abstract

The invention relates to a method for preparing lysosomal collagen, which is characterized by comprising the following steps of: after raw materials are subjected to treatment of impurity protein and fat removal, adding an acetic acid, citric acid or hydrochloric acid solution used as an extractant for extracting; adding pepsase; after extracting, filtering to obtain lysosomal collagen rough extract liquid; adding a sodium hydroxide solution into the lysosomal collagen rough extract liquid under the condition of stirring for adjusting the pH value to be 7.0-12; standing to obtain protein gel; dissolving the protein gel with the acetic acid, citric acid or hydrochloric acid solution to obtain a protein gel solution; and dialyzing with distilled water, freezing and drying to obtain the lysosomal collagen. The method disclosed by the invention is simpler and more reasonable, and the production cost is reduced due to no use of Tris in the method. Through the method, not only is the yield of the lysosomal collagen greatly increased, but also the heat stability of protein is maintained.

Description

technical field [0001] The invention relates to a method for preparing collagen, in particular to a method for preparing enzyme-soluble collagen. Background technique [0002] Collagen is the most important component of animal support structures, and in the human body is the most important protein of skin, blood vessels, bones, cartilage and connective tissues. Collagen is a large class of proteins. Collagen isolated from different tissues has very significant differences in peptide chains, amino acid composition, physicochemical properties, collagen configuration, etc. The present invention is mainly aimed at the most abundant I type collagen. At present, my country's collagen production is mainly obtained by extracting and purifying the skin and tendons of pigs and cattle. [0003] The extraction process of collagen in the prior art is basically the process of soaking raw materials for a long time at low temperature after removing impurities by sodium hydroxide. Generall...

Claims

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Application Information

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IPC IPC(8): C07K1/14
Inventor 段蕊张俊杰秦松蔡学东
Owner HUAIHAI INST OF TECH
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