10results about How to "High potency" patented technology

The invention relates to the technical field of immunization. The invention discloses parabacteroides dieldrii and parabacteroides goldsteinii, an application of the parabacteroides dieldrii and / or the parabacteroides goldsteinii in preparation of an immunopotentiator of a vaccine, and the immunopotentiator of the vaccine, and particularly discloses the parabacteroides dieldrii and the parabacteroides goldsteinii, the application of the parabacteroides dieldrii and / or the parabacteroides goldsteinii in preparation of the immunopotentiator of the vaccine. The parabacteroides dieldrii and / or the parabacteroides gold can be used as an immunopotentiator, can activate immunity, enhance antibody titer and shorten antibody generation reaction time, and can remarkably promote proliferation of B cells, plasma cells and memory B cell subgroups in a hair growth center.

The invention belongs to the field of microbial engineering, and provides a gentamicin C1a high-yield biological manufacturing method based on metabolic network modeling and reverse metabolic engineering renovation, which comprises the following steps: S1, constructing a genome scale metabolic model of a target production strain, predicting and screening a first target gene in positive correlation with gentamicin biosynthesis by using the model; s2, performing whole genome sequencing on the gentamicin high-yield mutant strain, and identifying a second target gene related to the high-yield character through comparative genomics analysis; s3, combining the first target gene predicted in the step S1 and the second target gene identified in the step S2, and constructing to obtain a recombinant engineering strain; s4, carrying out fermentation culture on the recombinant engineering strain, and supplementing materials in the fermentation process; by remarkably improving the production efficiency and strain performance of gentamicin C1a, important technical support and theoretical basis are provided for green biological manufacturing of antibiotics.

The present application relates to a kind of mycophenolic acid detection reagent and its preparation and detection method, specifically a kind of mycophenolic acid chemiluminescence immunoassay reagent and its preparation and detection method, comprising: anti mycophenolic acid specific antibody, for detecting the indicating reagent of anti mycophenolic acid specific antibody-mycophenolic acid combination;The above anti mycophenolic acid specific antibody is obtained by mycophenolic acid immunogen immunization animal.The present application has the advantages that: the mycophenolic acid immunogen of the present application is specific, and immunogenicity is high, and the prepared anti mycophenolic acid specific antibody is specific, and titer is high;Chemiluminescence immunoassay reagent containing the above anti mycophenolic acid specific antibody can determine the mycophenolic acid content in sample conveniently, quickly and accurately, and multiple samples can be simultaneously determined on full-automatic chemiluminescence immunoassay analyzer, realize the high-throughput rapid determination of mycophenolic acid, and the accuracy is high, and the specificity is strong, and accuracy and detection efficiency have been greatly improved.

This invention discloses a monoclonal antibody against amide alcohol drugs and its applications, belonging to the field of biotechnology. The monoclonal antibody against an amide alcohol drug includes a heavy chain variable region and a light chain variable region. The amino acid sequences of CDR1, CDR2, and CDR3 in the heavy chain variable region are shown in SEQ ID No. 5 to SEQ ID No. 7, respectively; the amino acid sequences of CDR1, CDR2, and CDR3 in the light chain variable region are shown in SEQ ID No. 8 to SEQ ID No. 10, respectively. This invention provides a high-titer, high-sensitivity monoclonal antibody against amide alcohol drugs. Test strips based on this antibody exhibit high sensitivity, providing strong technical support for the detection of amide alcohol drug residues, drug development, and quality control.

The application discloses a method for efficiently extracting heparin sodium from biological materials, and the method comprises the following steps: cleaning and crushing the raw material at low temperature; adding poloxamer, ammonium sulfate and complex enzymes to perform synergistic enzymolysis under mild and neutral conditions; after the reaction is completed, the temperature is increased to above the cloud point of the polymer, liquid-liquid two-phase separation is triggered, fat-soluble impurities are synchronously removed, and the water phase rich in heparin sodium is collected; after the water phase is purified through ion exchange, concentration, alcohol precipitation and vacuum drying are sequentially performed, then vibration screening and low-temperature drying are performed, and finally, high-purity heparin sodium powder is obtained. Through the intelligent phase change characteristics of the temperature-sensitive polymer, the high-efficiency enzymolysis and primary purification are coupled, the yield and purity of heparin are significantly improved, the high-salt, strong-alkali or high-temperature conditions in the traditional process are avoided, the biological activity of the product is effectively protected, and the yield is improved.

The application discloses a fentanyl artificial hapten, a fentanyl artificial antigen and a preparation method and application thereof. The fentanyl artificial hapten of the application retains the characteristic structure of fentanyl to the maximum extent, and has an active group which can be coupled with a carrier protein and can serve as an antigenic determinant; the fentanyl artificial antigen prepared further can be used for immunization to obtain an anti-fentanyl antibody with high affinity, high sensitivity and high specificity, and the titer of the immune serum obtained by immunizing a New Zealand white rabbit is as high as 1:85000. The antigen and antibody can be used for enzyme-linked immunoassay, immunochromatography and the like, have the characteristics of high sensitivity, high specificity, strong operability and the like, and can be used for screening and detection of fentanyl components in various complex samples.

The invention discloses a 6G3 monoclonal antibody and application thereof, the 6G3 monoclonal antibody is used for specifically binding a 6HB structural domain in a spike protein S2 subunit of SARS-CoV-2, the 6G3 monoclonal antibody comprises VHCDR1-3 of a heavy chain variable region and VLCDR1-3 of a light chain variable region, the amino acid sequences of VHCDR1, VHCDR2 and VHCDR3 are respectively shown as SEQ ID NO.1, SEQ ID NO.3 and SEQ ID NO.5, and the amino acid sequences of VLCDR1, VLCDR2 and VLCDR3 are respectively shown as SEQ ID NO.2, SEQ ID NO.4 and SEQ ID NO.6. The invention further discloses a preparation method of the 6G3 monoclonal antibody. The 6G3 monoclonal antibody can neutralize SARS-CoV-2 infection, and has important application value in serological detection of new coronavirus, preparation of a reagent for new coronavirus infection detection and a reagent for new coronavirus antigen or antibody detection.

The invention belongs to the technical field of immunology, and particularly relates to a monoclonal antibody pair aiming at helicobacter pylori adhesin A and application of the monoclonal antibody pair. The invention discloses an anti-HpaA monoclonal antibody pair which is named as an MM07 antibody and an MM14 antibody respectively, and discloses a corresponding coding gene, and a test strip and a kit containing the monoclonal antibody pair. The result of the embodiment shows that the monoclonal antibody pair disclosed by the invention has good specificity and sensitivity aiming at the HpaA antigen.

The application belongs to the technical field of biological detection, and discloses an anti-SpCas9 protein monoclonal antibody, a detection kit and application, wherein the heavy chain variable region amino acid sequence of the anti-SpCas9 protein monoclonal antibody is shown as SEQ ID NO. 1, and the light chain variable region amino acid sequence is shown as SEQ ID NO. 2; and a double-antibody sandwich ELISA detection kit is constructed by taking the monoclonal antibody as a capture antibody and an anti-SpCas9 protein polyclonal antibody as a detection antibody. The ELISA detection kit has the advantages of high sensitivity, strong specificity, good repeatability, high-throughput and rapid detection, can accurately quantify the expression and residual amount of SpCas9 protein in a gene editing biological sample, and provides strong support for the application and supervision of CRISPR / Cas9 gene editing technology.