135results about How to "High potency" patented technology

A segment of short-sequence polypeptide which can be separated in vitro is obtained through screening, and the short-sequence polypeptide is applied to polypeptide drugs such as analogues or derivatives of GLP-1, so that the stability of the polypeptide drugs can be improved, the action time of the polypeptide drugs can be prolonged, and the biological activity of the polypeptide drugs can be improved.

The invention relates to a Nipah virus N protein specific monoclonal antibody as well as a preparation method and application thereof. The nipah virus specific monoclonal antibody is prepared by immunizing nipah virus N protein, the amino acid sequence of the nipah virus N protein is as shown in SEQ ID NO.2, the nipah virus N protein is obtained by transcription of a nipah virus N gene, and the nucleotide sequence of the nipah virus N gene is as shown in SEQ ID NO.1. The nipah virus specific monoclonal antibody has the advantages that the nipah virus specific monoclonal antibody is prepared by immunizing the nipah virus N protein; the monoclonal antibody with the nipah virus N protein specificity can be specifically combined with the nipah virus N protein and is high in titer and good in affinity, meanwhile, a detection kit prepared based on the antibody can effectively carry out nipah virus detection, and technical support can be provided for epidemiological monitoring and diagnosis of nipah virus infection.

The invention relates to the technical field of biological agents, in particular to a goose astrovirus egg yolk antibody and a preparation method and application thereof. The invention provides a goose astrovirus egg yolk antibody. The goose astrovirus egg yolk antibody is prepared by adopting an inactivated vaccine as an antigen, wherein the inactivated vaccine is prepared from a goose astrovirus GAstV / SD / 36-95P strain with the preservation number of CCTCC (China Center For Type Culture Collection) NO: V202445. The neutralizing titer of the goose astrovirus egg yolk antibody provided by the invention is high and can reach 10.5 log2, after goslings are infected by the goose astrovirus, the goslings can be protected from disease attack and death by injecting the egg yolk antibody, and the protection rate of the egg yolk antibody can reach 100%.

The invention discloses a chimeric PEDV (porcine epidemic diarrhea virus) infectious clone plasmid, a PEDV chimeric virus as well as a construction method and application thereof. According to the invention, a PEDV genome is used as a skeleton for the first time, the chimeric virus for expressing the extracellular domain of the TGEV S protein is successfully rescued, and it is found that the TCID50 of the chimeric virus for expressing the extracellular domain of the TGEV S protein can be increased by 6.2 times compared with that of the chimeric PEDV for expressing the TGEV S protein. The recombinant virus is used as an inactivated vaccine for immunizing mice, so that the immunogenicity of TGEV S protein can be greatly improved, a body is stimulated to generate a higher-level neutralizing antibody aiming at TGEV, and the titer of the neutralizing antibody aiming at TGEV is improved by 16.8 times. Therefore, more possibilities are provided for modification of viral genomes, a novel method for improving immunogenicity of the TGEV inactivated vaccine is invented, and a basis is provided for research and development of the TGEV vaccine.

The invention discloses a preparation method of human urinary kallidinogenase for reducing bacterial endotoxin, which comprises the following steps: 1) dissolving a human urinary kallidinogenase crude product, and sequentially performing strong anion exchange column chromatography, hydrophobic column chromatography, virus inactivation and affinity column chromatography to obtain a human urinary kallidinogenase intermediate solution; (2) performing strong cation exchange column chromatography on the human urinary kallidinogenase intermediate solution obtained in the step (1), and eluting to obtain a bacterial endotoxin content of 1t and a unit titer of more than or equal to 15PNA unit / ml; and 0.16 EU / PNA unit of human urinary kallidinogenase. The human urinary kallidinogenase obtained by the preparation method has higher titer and lower bacterial endotoxin while ensuring high yield and high purity, is convenient for subsequent post-treatment and storage, and has higher safety during clinical use.

The invention discloses a peste des petits ruminants virus specific monoclonal antibody and application thereof.The monoclonal antibody is obtained by screening peste des petits ruminants virus N protein, the amino acid sequence of the peste des petits ruminants virus N protein is shown as SEQ ID NO: 1, the peste des petits ruminants virus N protein is obtained by transcription of a peste des petits ruminants virus N gene, and the peste des petits ruminants virus N gene is shown as SEQ ID NO: 2. The nucleotide sequence of the codon-optimized peste des petits ruminants virus N gene is shown as SEQ ID NO: 2. The monoclonal antibody provided by the invention can be specifically combined with the peste des petits ruminants virus N protein, the titer is high, the affinity is good, meanwhile, the detection kit prepared based on the antibody pair can effectively carry out peste des petits ruminants virus detection, and a technical support can be provided for epidemiological monitoring and diagnosis of peste des petits ruminants virus infection.

The invention discloses a Fel D1 and Fel D4 fusion protein and application thereof, the Fel D1 and Fel D4 fusion protein is obtained in a prokaryotic expression mode, the fusion expressed protein greatly simplifies operation procedures in the aspects of inducing protein expression, purification, concentration, immunization and the like, and the economic cost is reduced. According to the preparation method of the egg yolk antibody provided by the invention, the egg yolk antibody obtained through fusion protein immunization laying hens can be subjected to antibody-antigen reaction with Fel D1 and Fel D4 respectively, and the titer of the egg yolk antibody is higher than that of a single antigen protein immunization group and a combined immunization group. The invention further provides a preparation method of the functional cat food, the egg yolk powder is added into the cat food according to a certain proportion, after the cat food is fed for a period of time, the content of Fel D1 and Fel D4 in cat hair and saliva is remarkably reduced, the cat food prepared from the fusion protein egg yolk antibody can resist Fel D1 and Fel D4 at the same time, and the blocking effect is remarkably improved.

The invention relates to the technical field of biology, and aims to provide a monoclonal antibody 27E7 for resisting PCV2Cap protein as well as a preparation method and an application method of the monoclonal antibody 27E7. The monoclonal antibody comprises Ig structural domains VH CDR1, VH CDR2 and VH CDR3 of a heavy chain of the antibody, and Ig structural domains VL CDR1, VL CDR2 and VL CDR3 of a light chain of the antibody, wherein the amino acid sequences of the VH CDR1, the VH CDR2 and the VH CDR3 are respectively as shown in SEQ ID NO: 1 to SEQ ID NO: 3; the amino acid sequences of the VL CDR1, the VL CDR2 and the VL CDR3 are respectively as shown in SEQ ID NO: 4 to SEQ ID NO: 6. The monoclonal antibody provided by the invention is an IgG1 subtype, and has the advantages of high titer, strong specificity, high affinity and the like; the kit has high sensitivity and strong specificity, and has no cross reaction with other genotypes of porcine circoviruses; the kit can be further used for development of PCV2 diagnostic kits, and can be effectively popularized and applied to PCV2 diagnosis.

The invention discloses a wide-spectrum bacteriophage EFH2 capable of eliminating enterococcus faecalis suffering from humans and animals, and relates to the technical field of biology. The wide-spectrum bacteriophage is preserved in the China Center for Type Culture Collection on September 26, 2024, and the preservation number of the wide-spectrum bacteriophage is CCTCC NO: M 20242087. The wide-spectrum bacteriophage EFH2 is a newly found bacteriophage, has good tolerance under the conditions that the pH is 3-9 and the temperature is 4-60 DEG C, and has the titer as high as 2.31 * 10 < 8 > PFU / mL after being cultured for 8 hours under the condition that the MOI is 0.01. The wide-spectrum bacteriophage EFH2 is wide in antibacterial spectrum, short in incubation period, high in outbreak amount, capable of rapidly killing host bacteria, small in toxic and side effect, high in safety, wide in temperature and acid-base tolerance range, good in hydrophilicity and capable of well killing and disinfecting animals infected with multi-drug-resistant enterococcus and environments and food polluted by enterococcus.

The invention relates to the technical field of biology, in particular to a method for stably improving the titer of horse radish peroxidase labeled streptavidin. According to the method provided by the invention, streptavidin with different histidine label lengths is designed, and then the streptavidin is coupled with horse radish peroxidase oxidized by sodium periodate, so that high-titer horse radish peroxidase labeled streptavidin can be obtained. The invention also discloses application of the enzyme-labeled conjugate in a magnetic particle platform streptavidin-biotin system, and experimental results prove that the method provided by the invention can be used for stably preparing the enzyme conjugate with high titer and low background, and is widely applied in the streptavidin-biotin system.

The invention provides an anti-glycocholic acid antibody and application thereof, and particularly relates to more than ten positive monoclonal hybridoma cell strains obtained by adopting glycocholic acid antigen immune BALB / C mice and screening. Through performance determination, three cell strains capable of being specifically combined with glycocholic acid are finally obtained. Wherein the H15 antibody is high in titer, good in stability and stable in detection linearity, and can be used for clinical detection of glycocholic acid.

The invention relates to the technical field of antibodies, in particular to an antibody combined with Beclin1 and application of the antibody. The Beclin1 antibody provided by the invention can be specifically combined with a Beclin1 protein, has relatively high affinity to the Beclin1, and has relatively high titer. The antibody can be used for realizing specific, sensitive and accurate detection of the Beclin1, can be used for detecting the Beclin1 in practice, and has a relatively good application prospect.

The invention discloses a high temperature resistant pigeon-source Salmonella typhimurium phage, a phage composition and an application thereof, wherein the high temperature resistant pigeon-source Salmonella typhimurium phage is named PC204, and its deposit number is CGMCC No. 22368. The phage PC204 belongs to a long-tailed phage, has the characteristics of high temperature resistance, exhibits strong lysis and wide lysis spectrum characteristics to pigeon-source Salmonella typhimurium, can effectively prevent and treat pigeon-source Salmonella typhimurium diseases, has a specific lysis advantage to the pigeon-source Salmonella typhimurium, and can effectively reduce the mortality rate of pigeons and the positive rate of pigeon-source typhoid antibodies. It can be prepared into drugs for preventing and treating pigeon paratyphoid disease, environmental disinfectants, pigeon feed and pigeon water additives, and detection kits for application. The phage is safe and has no toxic side effects, will not produce drug-resistant bacteria, and has a good market application prospect.

The invention relates to the technical field of immunization. The invention discloses parabacteroides dieldrii and parabacteroides goldsteinii, an application of the parabacteroides dieldrii and / or the parabacteroides goldsteinii in preparation of an immunopotentiator of a vaccine, and the immunopotentiator of the vaccine, and particularly discloses the parabacteroides dieldrii and the parabacteroides goldsteinii, the application of the parabacteroides dieldrii and / or the parabacteroides goldsteinii in preparation of the immunopotentiator of the vaccine. The parabacteroides dieldrii and / or the parabacteroides gold can be used as an immunopotentiator, can activate immunity, enhance antibody titer and shorten antibody generation reaction time, and can remarkably promote proliferation of B cells, plasma cells and memory B cell subgroups in a hair growth center.

The present application provides a recombinant protein antigen of Acinetobacter baumannii BA71_01558, a preparation method and its application, and relates to the field of biomedical technology. The amino acid sequence of the recombinant protein antigen of Acinetobacter baumannii BA71_01558 of the present invention is shown in SEQ ID NO.1. The preparation process of the recombinant protein antigen of Acinetobacter baumannii BA71_01558 provided by the present application is simple, the operation steps are easy to control and easy to implement, and the obtained recombinant protein of Acinetobacter baumannii BA711_01558 has a high purity and a high yield, and is suitable for large-scale production. The vaccine prepared using the recombinant protein antigen of Acinetobacter baumannii BA711_01558 of the present application has no toxic side effects, can produce high-titer antibodies, has a good immune protection effect against Acinetobacter baumannii infection, and is suitable for preparing specific antibodies.

The invention belongs to the field of microbial engineering, and provides a gentamicin C1a high-yield biological manufacturing method based on metabolic network modeling and reverse metabolic engineering renovation, which comprises the following steps: S1, constructing a genome scale metabolic model of a target production strain, predicting and screening a first target gene in positive correlation with gentamicin biosynthesis by using the model; s2, performing whole genome sequencing on the gentamicin high-yield mutant strain, and identifying a second target gene related to the high-yield character through comparative genomics analysis; s3, combining the first target gene predicted in the step S1 and the second target gene identified in the step S2, and constructing to obtain a recombinant engineering strain; s4, carrying out fermentation culture on the recombinant engineering strain, and supplementing materials in the fermentation process; by remarkably improving the production efficiency and strain performance of gentamicin C1a, important technical support and theoretical basis are provided for green biological manufacturing of antibiotics.

The invention provides a bacteriophage freeze-drying protective agent, a bacteriophage freeze-drying powder, a preparation method and application, and belongs to the technical field of biological medicine preparations. The freeze-drying protective agent comprises the following components: a cryoprotectant, an antioxidant, an acid-base regulator and a filler. The freeze-drying protective agent disclosed by the invention is reasonably compounded, and the freeze-drying protective agent, the antioxidant, the pH regulator and the filler have a good protective effect on the bacteriophage through a synergistic effect, so that the inactivation rate or the death rate of the bacteriophage in a freeze-drying process can be effectively reduced, and the biological activity and the titer of the bacteriophage are improved. Moreover, the freeze-drying protective agent disclosed by the invention is relatively long in protective effect acting time, can improve the stability of the freeze-dried powder of the bacteriophage, improves the transportation and storage convenience of the freeze-dried powder of the bacteriophage, and effectively solves the problem that the freeze-dried powder of the bacteriophage is difficult to transport and store for a long time in practical application.

The present application relates to a kind of mycophenolic acid detection reagent and its preparation and detection method, specifically a kind of mycophenolic acid chemiluminescence immunoassay reagent and its preparation and detection method, comprising: anti mycophenolic acid specific antibody, for detecting the indicating reagent of anti mycophenolic acid specific antibody-mycophenolic acid combination;The above anti mycophenolic acid specific antibody is obtained by mycophenolic acid immunogen immunization animal.The present application has the advantages that: the mycophenolic acid immunogen of the present application is specific, and immunogenicity is high, and the prepared anti mycophenolic acid specific antibody is specific, and titer is high;Chemiluminescence immunoassay reagent containing the above anti mycophenolic acid specific antibody can determine the mycophenolic acid content in sample conveniently, quickly and accurately, and multiple samples can be simultaneously determined on full-automatic chemiluminescence immunoassay analyzer, realize the high-throughput rapid determination of mycophenolic acid, and the accuracy is high, and the specificity is strong, and accuracy and detection efficiency have been greatly improved.

The invention discloses an anti-avibacterium paragallinarum monoclonal antibody, an immunomagnetic bead marked by the monoclonal antibody and application of the immunomagnetic bead. The monoclonal antibody comprises a light chain variable region and a heavy chain variable region, the amino acid sequence of HCDR1 of the monoclonal antibody is as shown in SEQ ID NO: 9, the amino acid sequence of HCDR2 of the monoclonal antibody is as shown in SEQ ID NO: 10, the amino acid sequence of HCDR3 of the monoclonal antibody is as shown in SEQ ID NO: 11, the amino acid sequence of LCDR1 of the monoclonal antibody is as shown in SEQ ID NO: 3, the amino acid sequence of LCDR2 of the monoclonal antibody is as shown in SEQ ID NO: 4, and the amino acid sequence of LCDR3 of the monoclonal antibody is as shown in SEQ ID NO: 5. The monoclonal antibody is high in titer, strong in specificity and suitable for preparation of immunomagnetic beads based on the monoclonal antibody, and the prepared immunomagnetic beads have relatively high capture rate, relatively strong specificity and sensitivity to avibacterium paragallinarum.

The culture medium comprises methionine, a carbon source, a nitrogen source and a phosphorus source, and the contents of the methionine, the carbon source, the nitrogen source and the phosphorus source in the culture process are 0.3-0.5 g / L, 180-230 g / L, 19-27 g / L and 3-5 g / L respectively. The optimized culture medium for improving the yield of the intracellular antibiotics can be used for fermenting penicillium brevicompactum to produce mycophenolic acid, and the titer of the penicillium brevicompactum for producing the mycophenolic acid can be obviously improved in a short time by utilizing the optimized culture medium and the fermentation method which are prepared by the invention; by utilizing the culture medium prepared by the invention, the activity of the mycophenolic acid produced by the mould in the penicillium brevicompactum can be improved.

The present invention discloses a direct immunofluorescence reagent, a kit and an application thereof for detecting Cryptosporidium parvum, and belongs to the field of biotechnology. The present invention uses the whole worm protein of Cryptosporidium parvum as an antigen and adopts the hybridoma cell monoclonal antibody technology to prepare a monoclonal antibody against Cryptosporidium parvum; the monoclonal antibody against Cryptosporidium parvum obtained in step S1 is labeled with a fluorescent dye to obtain a direct immunofluorescence detection reagent for detecting Cryptosporidium parvum. The direct immunofluorescence detection method established by using the reagent of the present invention can be used for the detection and identification of Cryptosporidium parvum in clinical stool samples and the environment, and has high sensitivity, strong specificity, good stability, fast diagnosis speed, and good application prospects.

The invention discloses enterococcus faecium, a microbial inoculum as well as application and a method of the enterococcus faecium, the enterococcus faecium is enterococcus faecium TC3 and is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number of the enterococcus faecium is CCTCC NO: M 20242431. The invention also provides a microbial inoculum, wherein the microbial inoculum contains the enterococcus faecium TC3, and the enterococcus faecium TC3 can be used for preparing the microbial inoculum. Before a healthy pig is inoculated with a porcine reproductive and respiratory syndrome vaccine, the enterococcus faecium TC3 or the microbial inoculum is fed to the healthy pig, and then the healthy pig is inoculated with the porcine reproductive and respiratory syndrome vaccine, so that the effect performance of the pig inoculated with the porcine reproductive and respiratory syndrome vaccine can be improved, the antibody positive rate of the pig inoculated with the porcine reproductive and respiratory syndrome vaccine is increased, and the economic benefit of the pig is improved. The protection rate of the pigs inoculated with the porcine reproductive and respiratory syndrome vaccine is increased, the antibody dispersion of the pigs inoculated with the porcine reproductive and respiratory syndrome vaccine is reduced, and the survival rate of the pigs infected with the porcine reproductive and respiratory syndrome virus after the pigs inoculated with the porcine reproductive and respiratory syndrome vaccine is increased.

The invention discloses a method for improving biotin fermentation yield and stability by controlling temperature and humidity. The method comprises the following steps: inoculating a strain capable of synthesizing biotin into a solid culture medium, and culturing at constant temperature and humidity within the temperature range of 34-38 DEG C and the humidity range of 10-60% until the size of a bacterial colony or the thickness of a bacterial lawn is qualified; directly transferring the bacterial colony to a fermentation culture medium for culturing; or firstly transferring to a liquid culture medium and then transferring to a fermentation culture medium for fed-batch culture to finally obtain biotin. The method provided by the invention can effectively improve the titer of biotin produced by a fermentation method.

The present invention belongs to the field of biological detection, and discloses a monoclonal antibody for specific detection of MMP12 protein and its application. The monoclonal antibody for specific detection of MMP12 protein comprises a heavy chain variable region as shown in SEQ ID NO.7 and a light chain variable region as shown in SEQ ID NO.8. The monoclonal antibody for specific detection of MMP12 protein provided by the present invention has good biological activity, strong specificity, high titer, high sensitivity, and no cross-reactivity with other antigens, and has broad application prospects. The present invention also discloses a kit for specific detection of MMP12 protein, comprising the above-mentioned monoclonal antibody for specific detection of MMP12 protein, which can be used for specific detection of MMP12 protein in serum or whole blood, and can be used as an auxiliary diagnostic means for cancers with high expression of MMP12 protein, and has the advantages of simple detection method, high accuracy and low cost.

The invention belongs to the technical field of biological medicines, and particularly relates to an ATG16L1 monoclonal antibody and application thereof. The invention discloses an antibody specifically bound with ATG16L1 or an antigen binding fragment thereof, amino acid sequences of LCDR1-3 of a light chain variable region VL of the antibody or the antigen binding fragment thereof are respectively shown as SEQ ID NO: 3, 4 and 5, and amino acid sequences of HCDR1-3 of a heavy chain variable region VH of the antibody or the antigen binding fragment thereof are respectively shown as SEQ ID NO: 6, 7 and 8. The antibody disclosed by the invention has relatively high sensitivity and specificity.

The invention relates to a process method for fermenting gentamicin C1a based on ultrasonic assistance and resin adsorption. The method comprises the following steps: firstly, determining an optimal ultrasonic condition through an ultrasonic single-factor experiment; after the optimal ultrasonic condition is determined, gentamicin is promoted to be released from intracellular to extracellular due to the ultrasonic cavitation effect, so that extracellular gentamicin C1a is accumulated. In addition, in order to explore the influence of extracellular gentamicin C1a accumulation on production titer, a product addition experiment is designed. Then, resin is added at different time points in the fermentation process to adsorb extracellular accumulated gentamicin C1a, so that the purposes of reducing the inhibition effect of a product and improving the yield are achieved. And finally, the operation process is reasonably designed, and the process improvement effect after combination is verified.

The invention belongs to the technical field of genetically engineered bacteria and microbial fermentation, and particularly relates to a keto-alcohol acid reductoisomerase mutant, high-yield D-pantothenic acid bacteria and application, keto-alcohol acid reductoisomerase mutant is obtained by performing site-specific mutagenesis on 71st, 110th and / or 417th sites of keto-alcohol acid reductoisomerase, and the site-specific mutagenesis mode is that the 71st site is mutated into N, Q or Y from A, the 110th site is mutated into Y or R from Q, and the 71st site is mutated into N, Q or Y from R; the 417th site is mutated from A to Q, Y or R; the sequence of the keto-alcohol acid reductoisomerase is SEQ ID NO. 47; the production capacity of D-pantothenic acid is improved.

This invention discloses a monoclonal antibody against amide alcohol drugs and its applications, belonging to the field of biotechnology. The monoclonal antibody against an amide alcohol drug includes a heavy chain variable region and a light chain variable region. The amino acid sequences of CDR1, CDR2, and CDR3 in the heavy chain variable region are shown in SEQ ID No. 5 to SEQ ID No. 7, respectively; the amino acid sequences of CDR1, CDR2, and CDR3 in the light chain variable region are shown in SEQ ID No. 8 to SEQ ID No. 10, respectively. This invention provides a high-titer, high-sensitivity monoclonal antibody against amide alcohol drugs. Test strips based on this antibody exhibit high sensitivity, providing strong technical support for the detection of amide alcohol drug residues, drug development, and quality control.

The present application provides a recombinant protein antigen of Acinetobacter baumannii TonB, a preparation method and its application, and relates to the field of biomedical technology. The amino acid sequence of the recombinant protein antigen of Acinetobacter baumannii TonB of the present invention is shown in SEQ ID NO.1. The preparation process of the recombinant protein antigen of Acinetobacter baumannii TonB provided in the present application is simple, the operation steps are easy to control and easy to implement, and the obtained recombinant protein of Acinetobacter baumannii TonB has high purity and high yield, and is suitable for large-scale production. The vaccine prepared using the recombinant protein antigen of Acinetobacter baumannii TonB of the present application has no toxic side effects, can produce high-titer antibodies, has good immune protection effect against Acinetobacter baumannii infection, and is suitable for preparing specific antibodies.