3 results about "Golgi protein" patented technology

The invention provides an aggregation-induced emission (AIE) ionic probe (E)-4-(2-(5-(4-(diphenylamino) phenyl) thiophene-2-yl) vinyl)-1-(4-sulfanilamide acyl benzyl) quinoline-1-onium hexafluorophosphoric acid (V) salt of a targeted golf apparatus, namely a fluorescent probe TTQ-GA, a preparation method of the fluorescent probe TTQ-GA and application of the fluorescent probe TTQ-GA in the aspect of detection. The benzenesulfonamide group of the probe can be selectively combined with a Golgi apparatus protein cyclooxygenase 2 (COX-2) inhibitor SC-558 to realize Golgi apparatus targeting; due to the hydrophobic triphenylamine part and the hydrophilic quinoline cation part of the probe, the probe has amphipathy; the probe does not emit fluorescence in an aqueous solution, and can emit strong fluorescence when molecular movement is limited and excited, so that no-wash marking of the Golgi apparatus is realized; the probe generates a large amount of ROS under light excitation, promotes local lipid peroxidation increase of Golgi apparatus, and promotes cancer cell apoptosis.

The application discloses an anchor COX-2 protein responsive photosensitizer and a preparation method and application thereof, relates to the technical field of biological medicine and disease treatment. The compound IRID-SS shown in formula (I) prepared by the application is provided with a near-infrared cyanine as a photosensitive unit, an indomethacin as a COX-2 targeting ligand and a disulfide bond as a glutathione response unit. The compound can be activated under the condition of high glutathione in a tumor microenvironment, specifically anchors Golgi COX-2 protein, generates reactive oxygen species in situ through near-infrared laser irradiation, realizes irreversible oxidative damage of COX-2 protein, and induces pyroptosis of tumor cells through a Caspase-3 / GSDME channel. The compound has a simple synthesis method, does not introduce toxic reagents, does not have the problem of toxic reagent residue, has high biological safety, and therefore has good practical application value.

The present application provides a kind of polypeptide, polypeptide composition, kit and related application.The polypeptide is the polypeptide shown in SEQ ID NO:1 to SEQ ID NO:85, can be specifically bound as follows target antibody: human SNRPC / U1C antibody, human PCNA antibody, human CENPB antibody, human NUP210 / gp210 antibody, human FTCD / 58K golgi protein antibody and GAD antibody.The above-mentioned multiple specific binding target antibody polypeptide or polypeptide set can cover the peptide segment including linear epitope and nonlinear epitope, so that the number of polypeptides used can be reasonably selected according to the number of target antibodies to be detected, which can improve the detection sensitivity and specificity of each polypeptide, improve the detection accuracy, and improve the throughput and efficiency of the detected antibody.