10 results about "Golgi protein" patented technology

The present application relates to the field of antibody preparation and in vitro diagnostic reagent, and particularly relates to a GP73 protein scFV antibody prepared based on a phage display technology and an application of a magnetic microparticle chemiluminescence kit for detecting a GP73 content in human serum based on the antibody. Compared with the same type of product, the scFv antibody and the detection product developed based on the scFv antibody have a wider linear range and a better detection sensitivity, are simple to operate, have a detection time of no more than 30 minutes, and have a more stable and reliable detection result.

The invention provides an aggregation-induced emission (AIE) ionic probe (E)-4-(2-(5-(4-(diphenylamino) phenyl) thiophene-2-yl) vinyl)-1-(4-sulfanilamide acyl benzyl) quinoline-1-onium hexafluorophosphoric acid (V) salt of a targeted golf apparatus, namely a fluorescent probe TTQ-GA, a preparation method of the fluorescent probe TTQ-GA and application of the fluorescent probe TTQ-GA in the aspect of detection. The benzenesulfonamide group of the probe can be selectively combined with a Golgi apparatus protein cyclooxygenase 2 (COX-2) inhibitor SC-558 to realize Golgi apparatus targeting; due to the hydrophobic triphenylamine part and the hydrophilic quinoline cation part of the probe, the probe has amphipathy; the probe does not emit fluorescence in an aqueous solution, and can emit strong fluorescence when molecular movement is limited and excited, so that no-wash marking of the Golgi apparatus is realized; the probe generates a large amount of ROS under light excitation, promotes local lipid peroxidation increase of Golgi apparatus, and promotes cancer cell apoptosis.

The invention discloses a space-time marking method CAT-Golgi for a living cell Golgi proteome and application of the space-time marking method CAT-Golgi. The space-time labeling method comprises the following steps: sequentially adding a photosensitizer and an aniline probe into living cells to be labeled for incubation, and then irradiating the cells with green light with the wavelength of 520 + / -10nm to realize chemical labeling of a Golgi apparatus proteome, wherein the photosensitizer is a golgi apparatus positioning photosensitizer as shown in a formula I. The method disclosed by the invention can be used for efficiently, simply and conveniently carrying out golgi apparatus proteome capture on various cell lines (HeLa and K562), and compared with a traditional method, the method disclosed by the invention can be used for remarkably improving the subsecretion group specificity (greater than 40%). According to the method, CAT-Golgi proteome analysis can be carried out on cell lines and immune cells which are difficult to transfect.

The invention relates to an electrochemical detection method of GP73 based on a Fe-MOF nano material, which comprises the following steps: firstly, preparing high-performance Fe-MOF, and marking GP73AptI to form a signal probe; then, GP73Apt II is incubated on the surface of Au / SPCE to form a capture probe, and a 3D sandwich type space structure of GP73Apt I-GP73-GP73Apt II is constructed through a specific recognition effect. H2O2 oxidation is catalyzed through the peroxidase-like property of Fe-MOF, TMB is accelerated to be oxidized into oxTMB, oxidation current changes of TMB are obtained through DPV, high-specificity and high-sensitivity detection of GP73 is achieved, and the lowest detection limit reaches 9.78 ng / mL.

The application discloses an anchor COX-2 protein responsive photosensitizer and a preparation method and application thereof, relates to the technical field of biological medicine and disease treatment. The compound IRID-SS shown in formula (I) prepared by the application is provided with a near-infrared cyanine as a photosensitive unit, an indomethacin as a COX-2 targeting ligand and a disulfide bond as a glutathione response unit. The compound can be activated under the condition of high glutathione in a tumor microenvironment, specifically anchors Golgi COX-2 protein, generates reactive oxygen species in situ through near-infrared laser irradiation, realizes irreversible oxidative damage of COX-2 protein, and induces pyroptosis of tumor cells through a Caspase-3 / GSDME channel. The compound has a simple synthesis method, does not introduce toxic reagents, does not have the problem of toxic reagent residue, has high biological safety, and therefore has good practical application value.

The invention provides methods for determining or monitoring liver disease status. In particular, systems and methods are provided that employ a subjects age, sex, platelet count and concentrations of Golgi protein 73 (GP73) and laminin gamma-2 monomer (LG2m) to generate a liver disease score which is used to provide a liver disease status (e.g., absence of disease, presence, or stage of the disease).

The invention relates to a Golgi apparatus proteome analysis method of a chondroitin sulfate-based photocatalyst, and discloses a preparation method of a chondroitin sulfate (CS)-based photocatalyst and application of the material in Golgi apparatus proteome in-situ analysis. Under the excitation of visible light, the material is catalyzed to generate singlet oxygen or generate single electron transfer reaction, so that surrounding amino acid residues are activated and can be captured by substrate molecules containing nucleophilic amino groups and benzene rings. Proteins which are adjacently labeled are labeled by a substrate containing an alkynyl'chemical handle 'at the same time, and then are connected with breakable biotin molecules through click reaction, so that in-situ capture and identification of a Golgi apparatus proteome can be realized through streptavidin enrichment and liquid chromatography-mass spectrometry analysis; and identification of marker sites is realized through a proteomics strategy of open search. The method has the advantages of good biocompatibility, good selectivity, high temporal-spatial resolution, wide protein coverage range and the like.

The present application provides a kind of polypeptide, polypeptide composition, kit and related application.The polypeptide is the polypeptide shown in SEQ ID NO:1 to SEQ ID NO:85, can be specifically bound as follows target antibody: human SNRPC / U1C antibody, human PCNA antibody, human CENPB antibody, human NUP210 / gp210 antibody, human FTCD / 58K golgi protein antibody and GAD antibody.The above-mentioned multiple specific binding target antibody polypeptide or polypeptide set can cover the peptide segment including linear epitope and nonlinear epitope, so that the number of polypeptides used can be reasonably selected according to the number of target antibodies to be detected, which can improve the detection sensitivity and specificity of each polypeptide, improve the detection accuracy, and improve the throughput and efficiency of the detected antibody.

The invention relates to a novel chemical labeling method for identifying a Golgi apparatus proteome and a mediated secretory proteome thereof, namely a photocatalytic proximity marker based on an aggregation-induced emission (AIE) molecule. According to the method, a photosensitizer which has AIE characteristics and can efficiently target a Golgi apparatus is utilized to catalyze generation of singlet oxygen or single electron transfer reaction under excitation of visible light, so that surrounding amino acid residues are activated and can be captured by substrate molecules containing nucleophilic amino groups and unsaturated benzene rings. After the protein adjacent to the photosensitizer is marked by a substrate containing an alkynyl chemical handle, the protein is connected with a breakable biotin molecule through a click reaction, and in-situ capture and identification of a Golgi apparatus proteome and a mediated secreted proteome thereof can be realized through streptavidin enrichment and liquid chromatography-mass spectrometry analysis. And identification of marker sites is realized through a proteomics strategy of open search. The method has the advantages of good compatibility, good selectivity, high temporal-spatial resolution, wide protein coverage range and the like.

The invention belongs to the technical field of liver disease detection markers, and particularly relates to a biomarker combination for detecting liver cirrhosis, liver cancer, hepatitis combined with liver cancer and liver cirrhosis combined with liver cancer and application, the combined marker comprises chitosanase 3-like protein 1, alpha fetoprotein, alpha fetoprotein variant L3 ratio, abnormal prothrombin or Golgi protein 73, alpha fetoprotein, alpha fetoprotein variant L3 ratio, alpha fetoprotein variant L3 ratio and alpha fetoprotein variant L3 ratio. When the level of each component of the detected person is higher than a preset critical value, the corresponding disease is evaluated to be high in risk, and when the level of each component of the detected person is lower than the preset critical value, the corresponding disease is evaluated to be low in risk. The kit has the characteristics of high accuracy, strong specificity and high sensitivity, is also beneficial to detection of liver cirrhosis, liver cancer or hepatitis combined liver cancer which is not easy to detect, and can help patients to diagnose liver cirrhosis, liver cancer, hepatitis combined liver cancer or liver cirrhosis combined liver cancer as early as possible, so that the healing and survival probability is increased.