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AFP (Alpha Fetal Protein), GP (Golgi Protein)73 and PIVKA (Protein Induced By Vitamin K Absence)-II joint detection kit

A detection kit, -73GP73 technology, applied in the direction of biological testing, material inspection products, etc., can solve the problems of false negatives, achieve high specificity, quick and easy operation, and less hemolytic interference

Active Publication Date: 2015-03-11
ZYBIO INC
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, the use of serum AFP as a marker of HCC has a certain degree of false positives and false negatives

Method used

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  • AFP (Alpha Fetal Protein), GP (Golgi Protein)73 and PIVKA (Protein Induced By Vitamin K Absence)-II joint detection kit
  • AFP (Alpha Fetal Protein), GP (Golgi Protein)73 and PIVKA (Protein Induced By Vitamin K Absence)-II joint detection kit

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Embodiment 1

[0048] Preparation of test paper card of the present invention:

[0049] 1) Use the pretreatment buffer to pretreat the gold standard pad. The pretreatment buffer is: stachyose 2g / L, alum 0.5g / L, fructose sodium diphosphate 1.5g / L, sodium hexametaphosphate 0.3g / L L, glycine 1.88g / L aqueous solution, pH=7.4, the specific steps of pretreatment are: soak the gold standard pad in the pretreatment solution for 2 hours, take it out and dry it at 37°C; Protein AFP antibody, Golgi glycoprotein-73GP73 antibody, and protein PIVKA-II antibody solution induced by vitamin K deficiency or antagonist-II were sprayed on their corresponding pretreated gold standard pads to prepare AFP containing alpha-fetoprotein Antibody, Golgi glycoprotein-73GP73 antibody and gold label pad of protein PIVKA-II antibody induced by vitamin K deficiency or antagonist-II; the mass ratio of fluorescent microspheres to antibody in the solution is 5:1, and the concentration of the solution is 10mg / ml, the spray vo...

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Abstract

The invention relates to the field of biological detection, in particular to a detection kit for quantitatively detecting AFP (Alpha Fetal Protein), Golgi Protein-73GP73 and PIVKA (Protein Induced Vy Vitamin K Absence)-II or an antagonist-II as well as a preparation method and application of the detection kit. The detection kit comprises an AFP detection test paper card, a Golgi Protein-73GP73 detection test paper card and a PIVKA-II detection test paper card which are independent of one another, wherein each detection test paper card comprises a bottom plate, a sample pad, a gold-labeled pad, a nitrocellulose membrane and a water absorbing pad, wherein the sample pad, the gold-labeled pad, the nitrocellulose membrane and the water absorbing pad are positioned on the surface of the bottom plate and are arranged from the sample adding end in sequence. The kit provided by the invention has the benefits that the AFP, the GP73 and the PIVKA-II are firstly detected through a fluorescent microsphere immune chromatography, and both the sensitivity and the specificity are realized; the kit has the advantages of high quickness, simplicity and convenience in operation, accurate results, good economy and applicability and the like.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a combined detection kit for AFP, GP73 and PIVKA-II and its preparation method and application. Background technique [0002] Alpha fetoprotein (AFP) is a glycoprotein synthesized by the liver and yolk sac in the early stage of fetal development. With the growth of fetal development and postnatal age, the level of AFP in the blood gradually decreases. The reference range of adult serum AFP concentration 1-20μg / L. AFP is a sensitive and specific marker of hepatocellular carcinoma (HCC). If the serum AFP concentration exceeds 400 μg / L for 4 weeks or 200-400 μg / L for more than 5 weeks, hepatocellular carcinoma is highly suspected; at the same time, the serum AFP level is still low. It can be used to judge the size of liver cancer, evaluate the curative effect and judge the prognosis of liver cancer. However, according to statistics, about 20%-30% of patients with hepatocellular...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/68
Inventor 潘能科
Owner ZYBIO INC
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