172 results about "Nitrocellulose" patented technology

The invention discloses a porcine delta coronavirus monoclonal antibody and application thereof in preparation of an antigen detection test strip. The detection test strip comprises a polyvinyl chloride bottom plate on the lowest layer, and a sample pad, a conjugate pad, a nitrocellulose membrane and a water absorption pad are sequentially laid on the polyvinyl chloride bottom plate in the flowing direction; the sample pad is partially overlapped on the combination pad, and the combination pad and the water absorption pad are respectively overlapped on two sides of the nitrocellulose membrane; a detection line and a quality control line are arranged on the nitrocellulose membrane along the flowing direction; an antibody marked by red latex microspheres is sprayed on the combination pad, a coated antibody is sprayed on the detection line, and coated commercial goat anti-mouse IgG is sprayed on the quality control line. The test strip adopts a double-antibody sandwich method for detection, and has the advantages of high detection speed, high sensitivity, strong specificity and the like. The detection result is visual, clear and easy to interpret; the detection process does not need any instrument and equipment, operators do not need professional training, operation is easy and convenient, and the method is particularly suitable for on-site rapid diagnosis.

The invention discloses a preparation method and application of an HPV16 / 18 type E6 / E7 protein multi-target detection test strip, and belongs to the technical field of biological immunodetection analysis. The detection test strip comprises a back plate, a sample pad, a combination pad, a nitrocellulose membrane and an absorption pad, AIE fluorescent microsphere labeled probes are distributed on the combination pad, and a capture antibody and an AIE solution are distributed on the nitrocellulose membrane. The preparation method comprises the following three steps: preparation of the nitrocellulose membrane, preparation of the combination pad and assembly of the test strip. The detection test strip can synchronously and quantitatively detect PV16 E6, HPV16 E7, HPV18 E6 and HPV18 E7, each target corresponds to four signal points, multi-target synchronous rapid detection is achieved, the linear range is wide, the detection limit is low, operation is easy and convenient, cost is low, and the detection test strip is expected to become a novel efficient and reliable on-site screening tool for cervical cancer and precancerous lesions.

A rapid dual test kit (1) for DHA and EPA detection and method of preparation thereof; that detects DHA and EPA within 0-20 minutes, preferably 1 min; comprising of-a test device (2), a test diluent (29), a dropper (32), a pack insert (33) and a silica gel (34), an immuno-chromatographic strip (3) further comprising of a sample release pad (5), a conjugate pad (6), a nitrocellulose membrane (7), and an absorbent pad (8) arranged in a sequential overlapping manner over a backing material (4); encased in a test device (2) consisting of three open areas namely a sample port (11), test line viewing area (30), and control line viewing area (31); allowing lateral flow of sample (29) from sample release pad (5) to absorbent pad (8) via capillary action; and the method of preparation of kit is disclosed.

This utility model discloses a concentration device for the production of nitrocellulose, including a tank and an annular frame. An annular groove is formed on the inner wall of the annular frame, and a cross is rotatably connected to the inner side of the annular groove. A barrier frame is fixedly connected to the bottom of the cross, and a barrier net is fixedly connected between adjacent support members of the barrier frame. Two opposing lifting grooves are formed on the inner wall of the tank. Slider blocks are fixedly connected to the outer surfaces of both sides of the annular groove, and the two sliders are located inside the two lifting grooves and slidably connected to them. This utility model uses the barrier frame and barrier net to separate the material, thereby preventing the material from being carried out during liquid extraction, which could lead to blockage and material reduction, thus improving concentration efficiency.

This invention discloses a quantitative test strip for HBV PreS1 antibody based on the AIE molecule, its preparation method, and its applications, relating to the fields of molecular biology, immunology, and biodetection technology. The test strip includes a sample pad, a labeling pad, a nitrocellulose membrane (NC membrane), an absorbent pad, and a base plate. The NC membrane detection line is coated with HBV PreS1 antigen to capture PreS1 antibodies in the sample. The labeling pad contains an immobilized Anti-Human IgG antibody conjugated to the AIE molecule, which binds to the PreS1 antibodies in the sample to form an immune complex. The test strip of this invention enables rapid detection of PreS1 antibodies and achieves enhanced fluorescence in the detection results. Combined with a fluorescence reader or fluorescence card reader, the detection results can be digitally and quantitatively analyzed. It is simple to operate, rapid in detection, and significantly improves detection sensitivity and enables quantitative analysis.

A rapid test kit for Omega-3 fatty acid molecule detection and method of preparation thereof; for detection of DHA or EPA within one minute; comprising of - an immunochromatographic strip (3) further comprising of a sample release pad (5), a conjugate pad ( 6), a nitrocellulose membrane, and an absorbent pad, arranged in a sequential overlapping manner over a backing material; encased in a cassette (2) consisting of three open areas namely a sample port (11), test line viewing area (12), and control line viewing area; allowing lateral flow of sample from sample release pad (5) to absorbent pad via capillary action; and the method of preparation of kit includes steps of; cutting, coating of various pads and nitrocellulose membrane, preparation of capture and detector antibodies, lamination of plastic pad, assembly of cut laminates in cassettes, packing, sealing, preparing, dispensing test diluent into dropper bottle, packing sealed pouches and diluent bottles in boxes.

This invention relates to the field of biodetection technology, and more particularly to a fluorescent test strip for detecting canine parvovirus, along with hybridoma cells, monoclonal antibodies, and their applications. The test strip of this invention has fluorescent microspheres coated with canine parvovirus monoclonal antibody and fluorescently labeled rabbit IgG within the conjugate pad; a detection line and a control line are disposed on the surface of a nitrocellulose membrane, with the detection line coated with canine parvovirus monoclonal antibody and the control line coated with rabbit anti-IgG; wherein the canine parvovirus monoclonal antibody is secreted using hybridoma cells with accession number C202355. The test strip of this invention can be used to simultaneously detect feline parvovirus, canine parvovirus, and various genotypes of canine parvovirus, exhibiting high sensitivity and specificity, and possessing the technical advantages of accuracy, speed, and simplicity.

The present application relates to the technical field of light polyurethane varnish, especially to the field of IPC C09D175 / 175, more particularly to a clear finish for home products, a preparation method and application thereof. The components include: A component and B component, the weight ratio between the A component and the B component is 1:(0.3-0.6); the A component, by weight percentage, the raw materials include: 50-70% alkyd resin, 1-15% nitrocellulose, 1-5% polyamide wax, 1-5% additives, 3-8% matting powder, and the first solvent makes up the balance; the B component, by weight percentage, the raw materials include: 50-90% TDI curing agent, 0.1-2% dehydrating agent, and the second solvent makes up the balance. The short oil alkyd resin is used in combination with 3106-B-70 and 33089 from Changxing Chemical Industry, which can effectively solve the problem of large difference between positive and negative gloss in the actual production process.

The utility model discloses a fluorescent quantitative rapid detection card for ofloxacin quantum dot microspheres. The ofloxacin quantum dot microsphere fluorescent quantitative rapid detection card comprises a test strip, the test strip comprises a bottom plate, a sample pad, a fluorescent microsphere conjugate pad, a nitrocellulose membrane and a water absorption pad, the sample pad, the fluorescent microsphere conjugate pad, the nitrocellulose membrane and the water absorption pad are arranged on the bottom plate and connected in sequence, and detection lines and quality control lines which are distributed at intervals are arranged on the nitrocellulose membrane. The detection line is close to the fluorescent microsphere conjugate pad, the quality control line is close to the water absorption pad, the detection line is coated with a micromolecule ofloxacin antigen, the quality control line is coated with a goat anti-mouse IgG antibody, and a microsphere labeled antibody conjugate in the fluorescent microsphere conjugate pad is a fluorescent microsphere labeled ofloxacin antibody. The method is simple and rapid to operate, is less affected by environment temperature and solvent reagents, is suitable for rapid detection of food safety, and is accurate in detection result.

The invention discloses a micro gas generator homogeneous mixed medicament for a safety belt and a preparation method of the micro gas generator homogeneous mixed medicament, the micro gas generator homogeneous mixed medicament is prepared from the following components in percentage by mass: 90-97% of green gas generating medicament particles and 3-10% of nitrocellulose medicament particles uniformly dispersed in gaps of the green gas generating medicament particles, and the sum of the mass percentages of the components is 100%. By utilizing the advantages of high combustion speed and low ignition threshold of the nitrocellulose agent, the ignition delay and the combustion period of the mixed agent are shortened, the exposure time of the safety belt elbow in a high-temperature environment is shortened, the structural strength of the safety belt elbow is ensured, the problem of elbow explosion in a bonfire test is finally solved, and meanwhile, the environment-friendly characteristic of a green gas-producing agent is reserved; in addition, through the two-time charging process of the automatic production line, the mixed charging efficiency is high, and the product consistency is good.

The utility model provides a rapid detection kit for synchronously detecting cell factors IFN-gamma and TNF-alpha, comprising: a kit body comprising a card box and a box cover which covers the bottom of the card box; the detection card is arranged in the card box; the liquid suction device is connected with the detection card and extends out of the card box, and the box cover hermetically wraps the sample suction device; the detection card comprises a bottom plate and test strips adhered to the bottom plate, and the test strips comprise a first test strip and a second test strip which are symmetrically arranged on the two sides of the surface of the bottom plate; the first test strip comprises a sample pad, a first latex microsphere pad, a first nitrocellulose membrane and absorbent paper which are sequentially lapped; the second test strip comprises a sample pad, a second latex microsphere pad, a second nitrocellulose membrane and absorbent paper which are sequentially lapped. The kit is simple in structure, the kit comprises the two detection cards in parallel, IFN-gamma and TNF-alpha can be synchronously detected at a time, manpower and material resources are greatly saved, and operation is easy and fast.

The invention provides a progesterone detection reagent strip based on an antibody synergistic effect and a preparation method of the progesterone detection reagent strip. The progesterone detection reagent strip comprises a bottom plate, a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad, the combination pad is sprayed with fluorescent nano-microspheres coupled with a goat-derived PROG antibody and fluorescent nano-microspheres coupled with a goat anti-rabbit antibody, a detection line of the nitrocellulose membrane is coated with a PROG antigen, and a quality control line of the nitrocellulose membrane is coated with a rabbit anti-goat IgG antibody. According to the progesterone detection reagent strip based on the antibody synergistic effect, the competition method detection principle is adopted, the fluorescent microspheres are used as markers, the progesterone antibody and the fluorescent microspheres are combined in a coupling mode, the specificity and binding efficiency of antigen-antibody reaction are optimized based on the antibody synergistic effect, and the progesterone detection reagent strip can be used for detecting progesterone. The stability and sensitivity of the detection system are remarkably improved, and high-sensitivity and high-specificity detection of the progesterone level in serum or plasma is achieved.

The utility model discloses a detection kit for rapidly detecting chicken, duck and pork mixed in beef and mutton. The detection kit comprises a detection card and a plastic card, the detection card is formed by sequentially lining absorbent paper and a sample pad below a nitrocellulose membrane, cutting the nitrocellulose membrane into test strips, and packaging the test strips in the plastic card; due to the fact that specific species antibodies of chickens, ducks and pigs are preset in the detection kit, chicken, duck or pork components which are as low as 3% and mixed in milligram-level samples can be sensitively detected at a time through antigen-antibody reaction.

The application discloses a preparation method and application of an HPV16 / 18 type E6 / E7 protein multi-target detection test strip, and belongs to the technical field of biological immune detection and analysis. The test strip comprises a back plate, a sample pad, a combination pad, a nitrocellulose membrane and an absorption pad, AIE fluorescent microsphere labeled probes are distributed on the combination pad, and capture antibodies and AIE solution are distributed on the nitrocellulose membrane. The preparation method comprises three steps of preparation of the nitrocellulose membrane, preparation of the combination pad and assembly of the test strip. The test strip can quantitatively detect PV16 E6, HPV16 E7, HPV18 E6 and HPV18 E7 simultaneously, four signal points correspond to each target, multi-target synchronous rapid detection is realized, the linear range is wide, the detection limit is low, the operation is simple and convenient, the cost is low, and the test strip is expected to become a new tool for efficient and reliable on-site screening of cervical cancer and precancerous lesions.

The invention discloses a preparation method and application of a clothianidin fluorescent test strip, belongs to the technical field of agricultural detection, and solves the problems that the existing bombyx mori pesticide poisoning detection sensitivity is low, and the clothianidin residue concentration cannot be quickly judged. According to the method, a nitrocellulose membrane is prepared, a detection line is coated with a clothianidin-BSA antigen, and a quality control line is coated with an anti-mouse IgG antibody; when the fluorescent pad is prepared, carboxylated fluorescent microspheres and an anti-clothianidin antibody are coupled to form a labeled probe; when the test strip is assembled, the nitrocellulose membrane, the fluorescent pad, the sample pad and the absorbent paper are stacked and fixed on the bottom plate to form a chromatography structure. Clothianidin in a sample is combined with the labeled probe to form an immune complex, chromatography migrates to the detection line to be competitively combined with clothianidin-BSA antigen, the uncombined probe is captured and develops color, and the quality control line develops color through an anti-mouse IgG antibody. The test strip is used for rapidly detecting clothianidin residues in mulberry leaves and bombyx mori bodies, the sensitivity reaches 0.02 mg / L, pesticide poisoning events can be effectively prevented, and safe production of the sericulture industry is guaranteed.

To provide a printing ink composition for surface printing, which can form a printing layer having excellent adhesion to a substrate and excellent resistance to heat yellowing, even when the substrate is a polyethylene film that has not been subjected to corona discharge treatment. [Solution] A printing ink composition for surface printing, comprising a binder resin (A), a terpene resin (B), and an organic solvent, wherein the binder resin (A) comprises a nitrocellulose resin (A1) and a polyurethane resin (A2), the terpene resin (B) has a solubility parameter calculated by the Fedors method of 10.0 or less and a softening point of 90°C or more, and the content of the terpene resin (B) converted to solid content is 2 to 25 mass% relative to the total mass of the solid content of the printing ink composition for surface printing.

This application relates to the field of packaging paper technology, specifically to a flame-retardant cigarette tipping paper, comprising a substrate and a color layer and a flame-retardant layer laminated on the surface of the substrate; wherein, the flame-retardant layer comprises an electroplated aluminum layer and / or a metallic ink layer; the flame-retardant layer completely covers the color layer, or the flame-retardant layer partially covers the color layer in a strip shape with the covered portion close to the end of the flame-retardant cigarette tipping paper used for connecting with the cigarette, and the total width of the flame-retardant layer is ≥4mm; by mass percentage, the raw materials of the metallic ink layer include the following components: 7%–12% nitrocellulose resin, 4%–8% flame-retardant resin, 10%–20% metallic pigment, and 0.5%–2% additives, with the balance being solvent; the printing transfer amount of the metallic ink on the color layer is 1.5g / m 2 ~2.0g / m 2 The flame-retardant cigarette tipping paper provided in this application has a good flame-retardant effect; and the subsequent decoration process does not require additional color sequence, which can reduce production costs.

Lateral flow immunoassay systems include test strips having unique geometries to enhance fluid dynamics, including but not limited to: (1) conjugate pads having sinusoidal, meandering, and nozzle-diffuser patterns; and (2) nitrocellulose membrane pads having constriction zones corresponding to the location of T-lines and / or C-lines. The systems can further include a cartridge with a two-step rotation mechanism for contamination-free sample delivery to the sample pad of a test strip, and an AI-based detection system for quantifying the concentration of a detected analyte based on colorimetric signal intensities. The system exhibits improved analyte sensitivity over prior systems, reduces traditional assay time, eliminates subjective interpretation of colorimetric signal intensities, and quantifies analyte concentration levels.

The invention relates to the technical field of non-invasive glycosylated hemoglobin detection, and discloses non-invasive glycosylated hemoglobin detection test paper and method.The non-invasive glycosylated hemoglobin detection test paper comprises a sampling layer, a combination cushion layer is bonded to the lower surface of the sampling layer, and a nitrocellulose membrane layer is bonded to the side face, away from the sampling layer, of the combination cushion layer; a water absorption layer is adhered to one side surface, far away from the combined cushion layer, of the nitrocellulose membrane layer; a PVC bottom plate is adhered to one side surface, far away from the nitrocellulose membrane layer, of the water absorption layer; through cooperation of the structures, the pain feeling during sampling can be reduced, and the tissue fluid collecting efficiency is improved.

The invention discloses a test strip biosensor for detecting uranyl ions, the test strip biosensor comprises a reaction system and a test strip, the reaction system comprises a DNAzyme substrate chain and a DNAzyme enzyme chain; the test strip comprises a substrate, a sample pad, a coupling pad, a nitrocellulose membrane and an absorption pad, the sample pad, the coupling pad, the nitrocellulose membrane and the absorption pad are sequentially overlapped on the substrate, a gold nanoparticle-DNA probe I compound is fixed on the coupling pad, a test line and a control line are arranged on the nitrocellulose membrane, an SA-biotin-DNA probe II is fixed on the test line, and an SA-biotin-DNA probe II is fixed on the control line. And an SA-biotin-DNA probe III is fixed on the control line. Compared with the prior art, the biosensor can realize high-sensitivity and high-specificity detection of uranyl ions by integrating a DNA enzyme induced cleavage reaction and a gold nanoparticle mediated colorimetric detection technology, the detection process is simple and rapid, and an important technical support is provided for field monitoring of UO2 < 2 + > in environment and biological samples.

The application belongs to the technical field of paint, and provides a solvent type spraying anti-flocculation black paste, a preparation method and application thereof.The solvent type spraying anti-flocculation black paste comprises the following components in percentage by weight: aldehyde ketone resin, 5-10%; nitrocellulose, 5-15%; dispersing agent, 1-4%; modified carbon black powder, 5-10%; anti-sedimentation agent, 0.5-2%; mixed solvent, 65-80%.The solvent type spraying anti-flocculation black paste has excellent anti-sedimentation effect, good storage stability, can reduce color floating and flocculation in the spraying construction process, has excellent spraying anti-flocculation effect, and can greatly improve the spraying construction performance.

The application discloses a nitrocellulose feeding process, which comprises the following steps: adding a solvent, pouring the solvent into a stirring kettle; adding an additive, after the solvent is added, the additive is poured into the stirring kettle; feeding nitrocellulose, after the additive is added, the nitrocellulose is gradually fed into the stirring kettle according to the feeding sequence from low to high viscosity, so that the nitrocellulose is soaked in the solvent; pressing the nitrocellulose, the nitrocellulose is pressed downward by using a cotton pressing rod, so that the nitrocellulose is immersed in the solvent; and fully soaking, after the nitrocellulose is pressed, the nitrocellulose is statically soaked in the stirring kettle for more than 30 minutes. The feeding process of the nitrocellulose is optimized, the solvent is first poured into the stirring kettle instead of feeding the nitrocellulose first and then pouring the solvent, the cotton feeding process is slowed down, the nitrocellulose is sequentially fed according to the characteristics of the nitrocellulose, and the step of pressing the nitrocellulose is further added; after the nitrocellulose is completely immersed in the solvent, the method can reduce the risk of nitrocellulose production to the maximum extent and improve the safety of production.

The utility model relates to the field of biological detection, in particular to a urinary trypsinogen-2 determination kit, which is characterized in that a fluorescence labeled urinary trypsinogen-2 antibody is contained in a fluorescence labeled pad and can be quickly combined with urinary trypsinogen-2 in a sample; due to the design of the fluorescent labeled pad and the nitrocellulose membrane, the detection reaction is rapidly carried out, and the detection time is shortened; the sample pad, the fluorescent label pad, the compact glass fiber pad, the nitrocellulose membrane and the absorbent paper are reasonably arranged on the test strip, so that uniform flow of liquid on the test strip is ensured, and the detection efficiency is improved; a user only needs to dropwise add a sample into the sample adding opening, operation is easy and fast, complex pretreatment steps are not needed, even non-professional personnel can easily operate, and the use threshold is lowered. The problems that a traditional urinary trypsinogen-2 detection method is complex in operation and long in consumed time, needs professional technicians to carry out and limits wide clinical application of the traditional urinary trypsinogen-2 detection method are solved.

The utility model relates to the technical field of nitrocellulose membrane processing, and discloses nitrocellulose membrane production equipment which comprises a kettle body, a supporting seat arranged on the outer surface of the kettle body, a placing seat arranged on the upper surface of a kettle cover, a driving motor arranged on the upper surface of the placing seat, and a rotating shaft arranged at the output end of the driving motor through a coupler, one end of the rotating shaft penetrates through the kettle cover and extends into the kettle body, stirring blades are mounted on the outer surface of the rotating shaft, a plurality of mounting grooves are formed in the inner wall of the kettle body, baffles are arranged in the mounting grooves, a groove is formed between every two adjacent mounting grooves, and a connecting assembly is arranged in each groove. According to the utility model, a welding fixing mode in the prior art is replaced by the arranged connecting assembly, so that the baffle plate with plastic deformation can be conveniently and quickly replaced, the risk of fatigue failure of a welding seam caused by alternating stress is avoided, the physical damage of cutting replacement to the kettle body structure is eliminated, and a better practical effect is achieved.

The utility model relates to the technical field of recovery devices, and discloses a nitrocotton separation and recovery device which comprises a bottom plate, supporting rods are fixedly mounted at four corners of the lower surface of the bottom plate, and a baffle is arranged in the middle of the lower surface of the bottom plate; side plates are fixedly installed on the two sides of the upper surface of the bottom plate, a flow groove is formed between the bottom plate and the side plates, and a plate opening is formed in the middle in the bottom plate. And the baffle movably penetrates through the plate opening of the bottom plate. Through the arrangement of the baffle, the two sets of through openings and the sealing sleeve, the screw can be driven by the forward and reverse motor to rotate clockwise for several circles, the screw is matched with the supporting block to drive the baffle to move upwards, the baffle moves upwards to enable the through opening in the lower side and the filter screen to intercept nitrocotton, and when too much nitrocellulose is accumulated on the filter screen to block the nitrocotton, the motor drives the screw to rotate anticlockwise for several circles, so that the nitrocotton is blocked. Compared with a filter screen dismounting and mounting mode, the mode that the two sets of through openings are used alternately does not need to stop discharging waste water, and practicability is high.

The application discloses a test paper for detecting diethylstilbestrol and a preparation method thereof, and belongs to the technical field of small molecule analysis and detection. The test paper for detecting diethylstilbestrol comprises two parts of a test paper main body and a photothermal signal probe. The test paper main body comprises, in a horizontal direction, a sample pad, a nitrocellulose membrane (NC membrane) and an absorption pad which are sequentially and overlappedly pasted on a PVC base plate from top to bottom. The NC membrane comprises a detection zone (T zone) and a quality control zone (C zone). The T zone is fixed with diethylstilbestrol-BSA, and the C zone is fixed with a secondary antibody. The photothermal signal probe is a Bi2MoO6@NiS-mAb mixed solution. When the test paper is used for detecting diethylstilbestrol, the detection mode is flexible, the linear range is wide, the sensitivity is high, and the specificity is good.

This invention relates to a method for the large-scale production of "I"-shaped test strips for hygiene products, belonging to the field of medical test strip processing. The "I"-shaped test strip is an improvement and breakthrough of conventional test strips, reducing the foreign body sensation and other discomfort caused to users when used in hygiene products without affecting the test strip's detection function. The large-scale production process includes cutting colloidal gold-nitrocellulose boards, attaching colloidal gold-nitrocellulose strips to a substrate, attaching sample pads and absorbent pads to the substrate, laminating a top waterproof membrane, and rolling the formed test strips. This process can continuously produce high-quality "I"-shaped test strip rolls, meeting the diverse production needs of the hygiene product industry.

The invention discloses a high-gloss formula of nitro gloss paint for pencils and a preparation method, and particularly relates to the field of paint gloss paint. The coating comprises the following components: 18-22 parts of nitrocellulose with a nitrogen content of 12%, 8-12 parts of organosilicon modified resin, 5-8 parts of a gloss enhancing auxiliary agent, 2-4 parts of rare earth doped functional particles, 1.5-2.5 parts of a leveling agent, 1.5-2 parts of a cross-linking agent, and 50-60 parts of a solvent. The preparation method comprises the following steps: dissolving nitrocellulose and organosilicon modified resin; adding a gloss enhancing aid and rare earth doped functional particles, and dispersing to form a rare earth ion-silica chain-resin oriented network; the rare earth doped functional particles are prepared by doping a silica network with rare earth ions Ce < 3 + > and Eu < 3 + > and introducing an amino functional group, and the glossiness, the adhesive force, the wear resistance and the stability of a paint film are remarkably improved through the oriented network.

The invention relates to the technical field of nitrocotton processing, in particular to a closed micro-pressure discharge acid preparation machine which comprises a tank body, acid inlet pipes, a breather valve, a pressure detector and a controller, an acid outlet is formed in the bottom of the tank body, at least two acid inlet pipes are arranged at the top of the tank body, one end of each acid inlet pipe extends into the tank body, and the other end of each acid inlet pipe extends into the tank body. The breather valve is installed at the top of the tank body and communicated with the tank body, the pressure detector is installed at the top of the tank body, the detection end of the pressure detector extends into the tank body, and the controller is electrically connected with the breather valve and the pressure detector. The stirring device is arranged in the closed tank body, so that the acid preparation work can be completed in a limited closed space, the safety of the surrounding environment and personnel is guaranteed, meanwhile, the breather valve and the pressure detection device are arranged at the top of the tank body, overpressure is avoided, and the later-period nitrate smoke tail gas treatment pressure and treatment cost are reduced; and the treatment pressure caused by emission of a large amount of nitrate smoke is reduced.

The application discloses a preparation method of an up-conversion fluorescent chromatographic test strip for detecting azaspiracids (AZA), and belongs to the field of analytical chemistry, environment and food safety detection. The test strip comprises a sample pad, a binding pad, a nitrocellulose membrane, an absorption pad, a back plate, a detection line and a quality control line. The method utilizes up-conversion nanoparticles to improve the fluorescence intensity and reduce the background signal, and utilizes the conformational change of nucleic acid aptamer to realize the optical signal change of the detection line, thereby realizing the rapid quantitative detection of AZA. The test strip has the advantages of short detection time, simple operation, high specificity and high sensitivity, and has important significance for the detection of AZA in marine shellfish.