103 results about "Fluorescent microspheres" patented technology

The invention belongs to the technical field of biological detection, and provides a multiple cell factor detection kit in tumor immunity and a preparation method and application thereof, and the detection kit comprises a capture antibody, a detection antibody, a mixed protein standard substance, a diluent and a washing buffer solution. The capture antibody is prepared by coupling a biotinylated antibody with a fluorescent microsphere coated with biotin to obtain a capture antibody coupled with a fluorescent encoding microsphere, and the detection antibody is prepared by coupling derivatized phycoerythrin PE-SMCC with a specific thioether bond of a sulfhydrylated antibody to obtain a fluorescent labeled antibody; the mixed protein standard substance adopts a freeze-drying protection system containing BSA, trehalose and the like. The single hole of the kit can synchronously detect various cell factors, the sample dosage is only 25 microliters, the kit is suitable for immune monitoring of iron overload and other chronic inflammation models, compared with a traditional ELISA method, the efficiency is improved by 10 times, the cost is reduced, and the kit has the remarkable advantages of being high in throughput, wide in linear range and high in stability.

The invention belongs to the technical field of biological detection, and provides a multiple cell factor detection kit in macrophage polarization and a preparation method and application thereof, and the detection kit comprises a capture antibody, a detection antibody, a mixed protein standard substance, a diluent and a washing buffer solution. The capture system is used for preparing a capture antibody coupled with a fluorescence coding microsphere by coupling a biotinylated antibody with a fluorescence microsphere coated with biotin, and the detection system is used for preparing a fluorescence labeled antibody by coupling derivatized phycoerythrin PE-SMCC with a specific thioether bond of a sulfhydrylated antibody. The mixed protein standard substance adopts a freeze-drying protection system containing BSA, trehalose and the like. The single hole of the kit can synchronously detect multiple cell factors, the sample dosage is only 25 microliters, the kit is suitable for in-vitro tests of mouse mononuclear macrophage leukemia cell lines RAW264.7, compared with a traditional ELISA method, the efficiency is improved by 10 times, the cost is reduced, and the kit has the remarkable advantages of being high in throughput, wide in linear range and high in stability.

The invention discloses a preparation method and application of high-sensitivity and high-stability time-resolved fluorescent microspheres, and relates to the technical field of immunochromatography. The preparation method comprises the following steps: (1) preparing high-stability high-carboxyl-density polystyrene microspheres; (2) preparing a high-stability europium ion chelate; and (3) preparing the high-stability fluorescent microspheres. Through a strategy of combining functional monomer copolymerization and surface covalent labeling, the fluorescent microspheres which are connected through strong covalent bonds from inside to outside and are high in stability and carboxyl density are constructed; obvious differential advantages and innovativeness are shown in the aspects of chemical stability, anti-leakage capability of fluorescent markers and surface functional group density. A time-resolved fluorescent microsphere test strip developed based on the microspheres has the advantages of good stability, high detection sensitivity and the like, and can realize rapid detection and analysis of a substance to be detected.

The application discloses a detection method of cell surface antigens and application thereof, and relates to the detection field. The detection method of cell surface antigens comprises the following steps: mixing liposome-treated cells to be detected with capture microspheres and fluorescent microspheres, detecting the fluorescence intensity, and analyzing the expression of cell surface antigens. The surface of the capture microspheres is coated with a first antibody against a specific antigen on the surface of target cells. The surface of the fluorescent microspheres is coated with a second antibody against a target antigen on the surface of target cells. The detection method has low cost and can be used for directly quantitatively and qualitatively analyzing the expression of surface antigens of specific types of cells.

The invention discloses a biomarker combination related to hypoxia-induced neuroinflammation and application of the biomarker combination, and belongs to the technical field of biomarker detection. The marker combination disclosed by the invention consists of serum S100 beta protein, neuron-specific enolase, interleukin-6, high-mobility group protein B1 and neurofilament light-chain protein. The marker combination can be used for stratification of nervous system injury risk of severe hypoxia patients and acquisition of prognosis evaluation data. According to the detection method disclosed by the invention, synchronous quantitative detection of five markers is realized by adopting a multiple immunofluorescent microsphere technology, and a marker combination scoring algorithm is established to comprehensively evaluate the severity of the hypoxia-related neuroinflammation.

The application discloses a lateral test strip for detecting C-reactive protein and application thereof, and the test strip comprises a bottom plate and a sample pad, a combination pad, a nitrocellulose membrane and a water absorption paper which are sequentially stacked on the bottom plate; the combination pad is loaded with AIE fluorescent microspheres; the AIE fluorescent microspheres are composed of P(St-MMA) microspheres coated with AIE materials after hydrolysis and carboxylation; and anti-C-reactive protein antibodies are connected to the AIE fluorescent microspheres through a covalent bond. The surface coating amount of the AIE fluorescent microspheres is increased by 1.6-2.3 times, and the fluorescence intensity per unit mass is increased by about 40-60%. The increase of the coating amount makes the density of the light-emitting core higher, the overall quantum yield is significantly improved, and the uniformity of the fluorescence signal and the detection sensitivity are enhanced. The characteristics are particularly prominent in the detection of CRP protein in the chromatographic test strip, and the detection limit can be effectively reduced, the T / C signal ratio can be improved, and the reaction time can be shortened.

The application discloses a labeling method capable of improving fluorescence amplification effect and application thereof in biological toxin detection, and the labeling method comprises the following steps: S1, double-fluorescent microsphere preparation: S1.1, performing carboxyl site blocking on aflatoxin B1, vomitoxin and zearalenone antibodies; S1.2, performing coupling on amino sites of the closed aflatoxin B1, vomitoxin and zearalenone antibodies; S1.3, activating the coupled aflatoxin B1, vomitoxin and zearalenone antibodies; and S1.4, coupling the activated aflatoxin B1, vomitoxin and zearalenone antibodies with magnetic fluorescent microspheres. The labeling method capable of improving fluorescence amplification effect and application thereof in biological toxin detection have the advantages that the sensitivity and detection limit of products can be improved, and the detection difference caused by the interference of sample matrix can be reduced.

This invention relates to the field of biodetection technology, and more particularly to a fluorescent test strip for detecting canine parvovirus, along with hybridoma cells, monoclonal antibodies, and their applications. The test strip of this invention has fluorescent microspheres coated with canine parvovirus monoclonal antibody and fluorescently labeled rabbit IgG within the conjugate pad; a detection line and a control line are disposed on the surface of a nitrocellulose membrane, with the detection line coated with canine parvovirus monoclonal antibody and the control line coated with rabbit anti-IgG; wherein the canine parvovirus monoclonal antibody is secreted using hybridoma cells with accession number C202355. The test strip of this invention can be used to simultaneously detect feline parvovirus, canine parvovirus, and various genotypes of canine parvovirus, exhibiting high sensitivity and specificity, and possessing the technical advantages of accuracy, speed, and simplicity.

The invention relates to a synchronous detection method and system for small molecules and protein, and the synchronous detection method comprises the following steps: respectively preparing a small molecule detection antibody and a protein detection antibody marked by carboxyl fluorescent microspheres, and mixing the small molecule detection antibody and the protein detection antibody to obtain a mixed detection antibody; respectively preparing a microsphere-labeled capture micromolecule antigen and a protein antibody; preparing a microsphere array biochip with two detection areas; carrying out sample application on the microsphere-labeled captured micromolecule antigen and the protein antibody to the two detection areas; mixing a sample to be detected with the mixed detection antibody according to different proportions; slowly adding the sample mixed solution into two detection areas of the microsphere array biochip respectively; scanning and imaging the two detection areas of the microsphere array biochip respectively, identifying all fluorescent spot information in a fluorescent image, and obtaining the concentration of small molecules and proteins in a sample to be detected through a Poisson distribution principle or by establishing a standard curve. According to the invention, synchronous detection of small molecules and proteins can be realized.

The application discloses a high-brightness quantum dot fluorescent microsphere and a preparation method thereof based on an alkali catalysis strategy, and comprises the following steps: (1) preparing thiol-modified dendritic mesoporous silica microspheres; (2) assembling the thiol-modified dendritic mesoporous silica microspheres and quantum dots to obtain a dendritic silica / quantum dot assembly; (3) adding 3-mercaptopropyl triethoxysilane to the dendritic silica / quantum dot assembly, and performing organic silane modification on the dendritic silica / quantum dot assembly in a non-polar organic solvent by taking an organic alkali as an alkali catalyst; and (4) growing a silica shell layer on the surface of the organic silane-modified dendritic silica / quantum dot assembly by a method to obtain the high-brightness quantum dot fluorescent microsphere. The preparation method avoids fluorescence quenching of oil-phase quantum dots in the process of coating silica, and completely retains excellent optical properties of the oil-phase quantum dots.

The application discloses a preparation method and application of an HPV16 / 18 type E6 / E7 protein multi-target detection test strip, and belongs to the technical field of biological immune detection and analysis. The test strip comprises a back plate, a sample pad, a combination pad, a nitrocellulose membrane and an absorption pad, AIE fluorescent microsphere labeled probes are distributed on the combination pad, and capture antibodies and AIE solution are distributed on the nitrocellulose membrane. The preparation method comprises three steps of preparation of the nitrocellulose membrane, preparation of the combination pad and assembly of the test strip. The test strip can quantitatively detect PV16 E6, HPV16 E7, HPV18 E6 and HPV18 E7 simultaneously, four signal points correspond to each target, multi-target synchronous rapid detection is realized, the linear range is wide, the detection limit is low, the operation is simple and convenient, the cost is low, and the test strip is expected to become a new tool for efficient and reliable on-site screening of cervical cancer and precancerous lesions.

The invention discloses a fluorescence immunodetection analyzer and a detection analysis method thereof. The analyzer comprises a sheath flow pool which is vertically arranged, red and green laser excitation light sources which are horizontally arranged on the two sides of the sheath flow pool in the X-axis direction, and double independent light paths which are arranged on the two sides of the sheath flow pool in the Y-axis direction. A red laser light path separates fluorescence into three paths of signals of 730nm, 670nm and residual fluorescence through a series-connected dichotomy mirror group; the green laser light path collects diffuse reflection light. The four paths of optical signals are converted into voltage signals by four detectors, temperature compensation units integrated in the detectors correct the signals in real time based on a pre-calibrated dark current-temperature relation curve, and temperature drift is suppressed. And the signal processing system calls a pre-established signal model library to carry out comprehensive calibration and synchronous analysis on multi-channel signals, so that accurate distinguishing and counting of different fluorescent microspheres are realized. According to the invention, the problems of large temperature influence and low detection efficiency in the prior art are solved, and the stability, accuracy and multi-channel consistency of detection are remarkably improved.

The invention relates to the field of protein detection, in particular to a method for detecting phosphorylated Tau-217 protein in urine of an Alzheimer's disease patient, which comprises the following steps of: 1, collecting fresh urine in the morning by using a urine collecting container, and 2, taking out 100 microliters of urine by using a transfer pipette and adding the urine into a 5.0 ml centrifugal tube; step 3, adding 4.9 ml of a urine diluent to dilute the urine; and step 4, prefabricating tracing fluorescent microspheres and enrichment microspheres in the centrifugal tube. Step 5, repeatedly reversing and uniformly mixing, and reacting at room temperature for 8-12 hours; step 5, placing the reaction tube on a separation test tube rack with a magnet for more than 20 minutes; step 6, irradiating the test tube by using a highlighter with the wavelength of about 340nm in a dark environment to judge a result; and step 7, if a red fluorescent dot appears near the magnet in the test tube, the result is positive, otherwise, the result is negative, and the result can also be judged by measuring a fluorescence value through a luminoscope with the wavelength of 340nm. Finally, the phosphorylated Tau-217 protein is detected, and the operation threshold and the economic cost are reduced.

The invention relates to a micro-molecule detection method and system based on a micro-fluidic chip. The micro-molecule detection method comprises the following steps: preparing a detection antibody marked by carboxyl fluorescent microspheres; preparing a microsphere-labeled capture micromolecule antigen; preparing a micro-fluidic chip; transferring the prepared microsphere-labeled capture micromolecule antigen original sample to a detection area of a micro-fluidic chip; the method comprises the following steps: mixing a sample to be detected with a detection antibody according to different proportions, incubating for 1-10 minutes, and carrying out a competitive reaction to obtain mixed solutions with different proportions; slowly adding the mixed liquid with different proportions into a detection area of the micro-fluidic chip; slowly adding the cleaning solution into a detection area of the micro-fluidic chip; scanning and imaging a detection area of the micro-fluidic chip, inputting a fluorescence image obtained by scanning and imaging into the deep learning model, identifying all fluorescence spot information in the fluorescence image, and obtaining the concentration of the to-be-detected sample through the Poisson distribution principle or by establishing a standard curve. According to the invention, small molecules can be detected highly sensitively and rapidly.

The invention belongs to the technical field of detection, and particularly relates to an anti-swine IgA monoclonal antibody and application thereof in PEDV IgA antibody detection. The invention provides an anti-swine IgA monoclonal antibody and a sequence thereof, the monoclonal antibody is effective to swine IgA, but is ineffective to swine immune globulins IgG, IgM, IgD and IgE, and has good reactivity and specificity. According to the invention, an anti-swine IgA monoclonal antibody is labeled on lanthanide EU3 + fluorescent microspheres, and a time-resolved immunofluorescence test strip detection method is established and is used for qualitative detection of an IgA antibody. The detection method is wide in detection range, high in sensitivity and rapid in detection; good repeatability, accuracy and stability are achieved; in addition, the monoclonal antibody has no cross reaction with porcine immune globulins IgG, IgM, IgD and IgE, is high in specificity, has a clinical detection coincidence rate higher than 97.3%, and has strong clinical application potential.

The present disclosure provides systems, methods, and kits for improving the characterization (including quality control) of flow cytometers. In one embodiment, a system for characterizing a flow cytometer includes a first standard particle reagent (the first standard particle reagent includes a first particle mixture) and a second standard particle reagent (the second standard particle reagent includes a second particle mixture, the particles having a fluorescent dye). The systems, methods, and kits of the present invention offer significant advantages over currently available fluorospheres used for quality control of flow cytometers prior to evaluating nanoparticles (e.g., EVs).

The invention provides a D-dimer detection kit and a preparation method thereof. A chromatographic test strip of the kit comprises a bottom plate, a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad. And the combination pad is coated with a D-dimer detection antibody marked by carboxylated time-resolved fluorescent microspheres. A detection line of the nitrocellulose membrane is coated with a D-dimer capture antibody, and a quality control line of the nitrocellulose membrane is coated with a goat anti-mouse IgG antibody. The D-dimer detection antibody and the D-dimer capture antibody are a pair of monoclonal antibodies for specifically recognizing different epitopes of the D-dimer. According to the kit, the convenience of immunochromatography and the high sensitivity characteristic of the time-resolved fluorescent microspheres are fused, accurate and quantitative detection of the D-dimer is achieved through the synergistic effect of the detection antibody and the capture antibody which specifically recognize different epitopes, and the kit is high in sensitivity, good in specificity, easy and convenient to operate, high in anti-interference capacity and high in sensitivity. The clinical high-precision quantitative monitoring requirement can be met, and the diagnosis and treatment application value and the market prospect are achieved.

The invention discloses an echinococcus multilocularis monoclonal antibody and a fluorescent microsphere immunochromatography detection test strip, and relates to the technical field of monoclonal antibodies. The echinococcus multilocularis monoclonal antibody is an echinococcus multilocularis monoclonal antibody 13H11F4, the amino acid sequence of a heavy chain of the echinococcus multilocularis monoclonal antibody is shown as SEQ ID NO.1, and the amino acid sequence of a light chain of the echinococcus multilocularis monoclonal antibody is shown as SEQ ID NO.2. The monoclonal antibody 13H11F4 of the echinococcus multilocularis can be combined with other monoclonal antibodies of the echinococcus multilocularis, so that the echinococcus multilocularis can be detected by a double-antibody sandwich method. The fluorescent microsphere immunochromatography test strip prepared from 13H11F4 and other monoclonal antibodies of other echinococcosis multilocularis is used for medical detection of echinococcosis multilocularis, has the advantages of high sensitivity, short reaction time, strong anti-interference capability and accurate and reliable detection result, and has wide clinical application value.

The invention discloses a method for rapidly detecting malachite green in aquatic products, and belongs to the technical field of biological detection. According to the time-resolved fluorescent microsphere immunochromatography kit based on the specific nano-antibody, the nano-antibody is applied to immunochromatography, an optimized pretreatment method is combined, malachite green in aquatic products can be rapidly detected on site, sample pretreatment and detection can be completed within 15 min, the limit of detection (LOQ) is 0.03 mu g / kg, and the time-resolved fluorescent microsphere immunochromatography kit can be used for detecting malachite green in aquatic products. The naked eye line elimination value is 1.6 mu g / kg which is far higher than the detection level in the prior art, and the method has the advantages of high sensitivity, good specificity, simplicity in operation, short detection time and the like, and is suitable for on-site rapid screening of malachite green residues in aquatic products.

The invention discloses a time-resolved microsphere and a preparation method thereof. The preparation method comprises the following steps: S1, preparing a europium complex; s2, preparing white carboxyl latex microspheres; and S3, preparing the time-resolved microspheres by a swelling method. The time-resolved fluorescent microspheres prepared by the invention have the characteristics of high brightness, ultra-long fluorescence lifetime, excellent stability, good biocompatibility, easiness in functionalization and the like, so that the potential of a time-resolved fluorescence immunoassay technology is fully exerted, and the ever-increasing requirements of high sensitivity, high accuracy and rapid detection are met.

The invention discloses a method for detecting leakage points of an underground complex buried pipe multi-water supply system. The method is suitable for a scene in which a plurality of independent water supply subsystems share a drainage path and need to be diagnosed without interruption. A composite tracer agent with a unique multi-mode code is injected into each subsystem, and signals are synchronously detected at a water seepage point; screening candidate subsystems based on the pipe network topology model and the positions of the water seepage points, and identifying leakage sources by adopting a weighted Bayesian model; then slow-release fluorescent microspheres are injected, an unmanned aerial vehicle or a ground sensing network is used for collecting ground surface fluorescence distribution, and leakage point coordinates are inverted based on a Darcy law coupling convection-diffusion equation. According to the method, shutdown excavation is not needed, the positioning precision reaches the sub-meter level, the problems of signal aliasing, environment interference, positioning fuzziness and the like can be solved on the premise that operation of any water supply subsystem is not interrupted, and accurate identification of a leakage source and accurate positioning of a leakage point are achieved.

The application discloses a copper ion removing and detecting reagent based on cluster luminescent polymer microspheres, a preparation method and application thereof, and the reagent is obtained by sulfonating monodisperse porous cross-linked polystyrene microspheres, and the reagent is used for removing and specifically detecting copper ions. The one-step sulfonating monodisperse porous cross-linked polystyrene microsphere method is used to prepare the copper ion removing and detecting reagent based on cluster luminescent polymer microspheres. The polymer fluorescent microspheres based on cluster luminescence have excellent detection capacity for copper ions. Meanwhile, the sulfonic acid groups after the sulfonation treatment make the polymer microspheres have excellent copper ion removing capacity. The application provides a new method for copper ion detection and removal, and the new method has the advantages of simple operation, economy and good stability.

This invention discloses a method for preparing and applying highly sensitive and stable time-resolved fluorescent microspheres, relating to the field of immunochromatography. The preparation method of this invention includes the following steps: (1) preparation of highly stable polystyrene microspheres with high carboxyl density; (2) preparation of highly stable europium ion chelates; and (3) preparation of highly stable fluorescent microspheres. This invention constructs a highly stable fluorescent microsphere with high carboxyl density, linked by strong covalent bonds from the inside out, through a strategy combining "functional monomer copolymerization" and "surface covalent labeling." This microsphere exhibits significant advantages and innovation in chemical stability, anti-leakage capability of the fluorescent label, and surface functional group density. The time-resolved fluorescent microsphere test strip developed based on this microsphere has advantages such as good stability and high detection sensitivity, enabling rapid detection and analysis of analytes.

The application relates to the technical field of analysis and detection, and discloses an echo wall mode sensor and a preparation method and application thereof. The echo wall mode sensor comprises a substrate and a fluorescent layer arranged on the substrate, and an inert layer is further arranged on the fluorescent layer; the fluorescent layer comprises an inner fluorescent layer and an outer fluorescent layer; the concentration of fluorescent dye in the inner fluorescent layer is 0.05-0.4 mg / mL; and the concentration of fluorescent dye in the outer fluorescent layer is 0.3-1.2 mg / mL. Through the technical scheme, the problems of low signal-to-noise ratio and poor displacement stability of an echo wall mode sensor based on fluorescent microspheres in the related art are solved.

The invention discloses an echinococcus multilocularis monoclonal antibody and a fluorescent microsphere immunochromatography detection test strip, and relates to the technical field of monoclonal antibodies. The echinococcus multilocularis monoclonal antibody comprises an echinococcus multilocularis monoclonal antibody 20B7D9 and an echinococcus multilocularis monoclonal antibody 18F11G9, and the echinococcus multilocularis monoclonal antibody 20B7D9 and the echinococcus The echinococcus multilocularis monoclonal antibody 20B7D9 can be combined with the echinococcus multilocularis monoclonal antibody 18F11G9, so that the echinococcus multilocularis can be detected by a double-antibody sandwich method. The fluorescent microsphere immunochromatography test strip prepared from the 20B7D9 and the echinococcosis multilocularis monoclonal antibody 18F11G9 is used for medical detection of echinococcosis multilocularis, has the advantages of high sensitivity, short reaction time, strong anti-interference capability and accurate and reliable detection result, and has wide clinical application value.

The invention provides an avibactam / ceftazidime blood concentration rapid joint detection test strip and a preparation method, system and application thereof. The system comprises an immunochromatography test strip and a time-resolved fluorescence detection system. The immunochromatography test strip comprises a sample pad, a combination pad, a nitrocellulose membrane detection area and an absorption pad; the sample pad is made of glass fibers treated by cane sugar or a surfactant; the combination pad is loaded with a time-resolved fluorescent microsphere coupled monoclonal antibody probe; an avibactam detection line T1, a ceftazidime detection line T2 and a quality control line C are arranged in the nitrocellulose membrane detection area according to the flowing direction; the absorption pad is made of cellulose materials. Compared with the prior art, rapid combined detection of the avibactam / ceftazidime blood concentration can be realized within 15 minutes, and the method has the advantages of high sensitivity, wide linear range, strong anti-interference capability and simplicity and convenience in operation, and can meet the bedside rapid detection requirement.

The application discloses a kind of based on nano antibody-monoclonal sandwich detection novel coronavirus receptor binding domain protein homogeneous method, it is related to antigen detection field.The expression vector of novel coronavirus RBD protein, nano antibody is constructed to carry out protein expression, the pairing performance of nano antibody and monoclonal antibody is identified by ELISA method, nano antibody and monoclonal antibody are coupled to the surface of fluorescent microsphere and magnetic nanometer microsphere respectively, novel coronavirus RBD protein is diluted into different concentrations using PBS buffer solution, is input into the fluorescent and magnetic nano probe of target RBD protein, fluorescence value is determined after being separated by magnetic frame, and the standard curve for detecting novel coronavirus RBD protein is established, and the minimum detection line is 0.09ng / mL.The immunofluorescence homogeneous method constructed by the application is simple in operation, fast in detection speed, less in steps, low in cost, suitable for a variety of scene detection, and lays a foundation for the development of novel coronavirus rapid detection kit.

The invention belongs to the technical field of biological detection, and discloses an allergen-specific IgE antibody detection card, a kit and application. The allergen specificity IgE antibody detection card comprises a combination pad and a nitrocellulose membrane, wherein the combination pad is coated with a mouse anti-human IgE antibody marked by AIE polystyrene fluorescent microspheres and a rabbit IgG antibody marked by AIE polystyrene fluorescent microspheres; a detection line of the nitrocellulose membrane is coated with a specific antigen, and a quality control line of the nitrocellulose membrane is coated with a goat anti-rabbit IgG antibody. The kit prepared from the reagent card is used for detecting the allergen-specific IgE antibody, has the characteristics of high detection sensitivity, high specificity, simplicity in operation, cost saving and the like, and has a very large market and a good application prospect.

The invention relates to the field of medical detection and discloses a colorectal cancer detection kit as well as a preparation method and a use method thereof. The colorectal cancer detection kit comprises a fluorescence labeling material and a lateral chromatography test strip, the fluorescent labeling material comprises fluorescent microspheres, an antibody corresponding to the colorectal cancer exosome marker and a primary antibody of an independent C line, wherein the antibody and the primary antibody are connected with the fluorescent microspheres; the lateral chromatography test strip comprises a composite pad, a detection membrane and a water absorption pad; one end of the detection film is lapped with the composite pad, and the other end is lapped with the water absorption pad; a T line close to the composite pad and a C line close to the water absorption pad are arranged on the detection film; the T line is coated with an exosome marker protein corresponding antibody; the C line is coated with a secondary antibody of an independent C line; the fluorescence labeling material and the lateral chromatography test strip are independently stored, or the fluorescence labeling material is attached to a composite pad of the lateral chromatography test strip. The colorectal cancer detection kit can realize detection of colorectal cancer marker protein, and further realizes detection of colorectal cancer.