96 results about "Brucellosis" patented technology

The invention relates to the technical field of space-time prediction, in particular to a brucellosis space-time prediction method and system based on artificial intelligence, and the method comprises the following steps: obtaining case information, calculating an incidence relation, extracting a path sequence, screening a trend direction, and matching a new case to generate prediction data. According to the method, continuous identification of a propagation path is realized by constructing a propagation incidence relation between cases and introducing a spatial directivity index, a non-trend propagation process is screened out through an angle average value and a variance, spatial consistency of path screening is enhanced, and through joint matching of spatio-temporal characteristics of newly-added cases and an existing propagation trend, the propagation path screening efficiency is improved. The sensitivity of prediction to the trend attribution of a new case is improved, the spatial directivity of a potential disease area is enhanced through reverse projection of a trend path and area positioning drop point frequency analysis, and through linkage processing of multi-level path extraction, trend judgment and area coding, the probability of occurrence of the new case is lowered. And the capturing capability of the prediction data on the propagation and evolution characteristics of the brucellosis is improved.

The invention discloses a primer group, a kit and a method for detecting brucella, and belongs to the technical field of gene detection. The primer group comprises two pairs of specific RPA (recombinase polymerase amplification) primers: a primer pair B1F1 (SEQ ID No.3) and a primer pair B1R1 (SEQ ID No.6) targeting a B1 sequence, and a primer pair B2F1 (SEQ ID No.8) and a primer pair B2R1 (SEQ ID No.11) targeting a B2 sequence. The invention also provides a kit containing the primer group, and a detection method based on the RPA-CRISPR-Cas12a. The invention also provides a kit containing the primer group and a detection method based on the RPA-CRISPR-Cas12a. According to the application, the high efficiency of RPA amplification is combined with the ultrahigh specificity of CRISPR-Cas12a detection, the defects of long time consumption, low sensitivity and easy generation of false positive in the traditional method are overcome, rapid, sensitive and specific detection of Brucella is realized, and the application has important application value in early screening and monitoring of Brucella.

The invention relates to the technical field of risk monitoring, in particular to a brucellosis risk monitoring and early warning method based on remote sensing and GIS (Geographic Information System), which comprises the following steps of: acquiring multi-stage remote sensing images to construct an ecological grid set, hanging cases, positioning and generating a disease source boundary map layer, identifying a high vegetation and temperature intersection area to form a risk structure, and carrying out early warning on the risk structure. And extracting the display area and superposing the GIS map to output early warning information. According to the method, the multi-stage remote sensing image covering the key epidemic area is obtained, region segmentation and geocoding registration are executed, by means of coupling detection of the normalized vegetation index and the land surface temperature, the spatial intersection region with the pathogen propagation suitable ecological condition can be identified, the risk index is calculated according to the graph block structure distribution, and the risk level label is given; according to the method, accurate positioning and hierarchical evaluation of a high-risk ecological environment and historical case space overlapping region are realized, the space prediction precision and the intuition of risk prompt are improved, and the capability of responding to the brucellosis potential outbreak trend in advance is effectively enhanced.

The invention relates to a silicon-based nano material for targeted therapy of brucellosis as well as a preparation method and application of the silicon-based nano material. The invention relates to a preparation method of a silicon-based nano material for targeted therapy of brucellosis. The preparation method comprises the following steps: (1) preparing magnetic mesoporous silica; (2) aminating the magnetic mesoporous silica, and modifying the magnetic mesoporous silica with a responsive material and yeast beta-glucan to obtain modified magnetic mesoporous silica; and (3) carrying out antibiotic loading on the modified magnetic mesoporous silica. According to the silicon-based nano material for targeted therapy of brucellosis as well as the preparation method and the application of the silicon-based nano material, the surface of magnetic macroporous mesoporous silica is modified with yeast beta-glucan capable of targeting M cells and glutathione responsive molecules, and a drug delivery system sensitive to a brucellosis environment is synthesized; the nano-carrier has targeted targeting selectivity for delivering and releasing drugs, the activity, slow release and target cell uptake efficiency of antibiotics are ensured, and the brucellosis treatment is more accurate and efficient.

The invention relates to the technical field of markers, in particular to application of a biomarker for identifying acute stage and chronic stage of brucellosis. A series of biomarkers related to acute and chronic stages of brucellosis are screened and found, and the biomarkers have obvious difference between acute and chronic brucellosis patients. The biomarkers can realize acute and chronic staging of brucellosis, are high in sensitivity and specificity, and have relatively good diagnosis efficiency. A novel molecular diagnosis tool is provided for acute and chronic staging of brucellosis, the problems that existing serological detection is low in specificity, and clinical staging cannot be accurately judged are solved, and wide application prospects are achieved.

The invention is applicable to the technical field of genetic engineering and biological medicine, and provides construction of a fusion recombinant protein ABT for preventing brucellosis and application of the fusion recombinant protein ABT in preparation of protective vaccines. The fusion recombinant protein ABT is composed of an immunological enhancement antigen and a multi-epitope tandem antigen, wherein the immunological enhancement antigen (named as A) comprises a Brucella ribosome L7 / L12 protein and a PADRE sequence; the multi-epitope tandem antigen is formed by connecting a dominant B cell epitope part (named as B), a dominant Tc cell epitope part and a dominant Th cell epitope part (named as T) in series, wherein the dominant B cell epitope part (named as B) is derived from Brucella SurA, OMP31, BP26 and Trigger factor proteins. The ABT Brucella multi-epitope subunit vaccine prepared by mixing the fusion recombinant protein ABT and an immunologic adjuvant has the characteristics of good purity, high safety and strong immunogenicity, can stimulate an organism to generate a protective antibody, and has long antibody maintenance time.

The invention discloses a preparation method of manganese dioxide nano-enzyme and application of the manganese dioxide nano-enzyme in a disease detection kit.The preparation method of the manganese dioxide nano-enzyme comprises the following steps that a fish peptone solution containing fish peptone powder and a potassium permanganate aqueous solution are mixed to be uniform, a mixed solution is obtained, the mixed solution is stirred to be brown, and a manganese dioxide nano-enzyme solution is obtained; the manganese dioxide nano-enzyme has an ultra-small particle size of 2-5nm, overcomes the problem that the traditional nano-enzyme is small in particle size and easy to agglomerate, and has excellent dispersity and stability; in addition, the manganese dioxide nano-enzyme prepared from the fish peptone powder has high peroxidase-like activity and stability, and the enzyme activity of the manganese dioxide nano-enzyme is 2.36 times higher than that of HRP in a brucellosis detection ELISA kit.

Provided herein are methods and compositions for the detection of Brucella antigen, and more particularly, to a vaccine or immunogenic composition comprising: Brucella strain from which at least a portion of the immunogenic protein DUF883 has been deleted provided in an amount sufficient to trigger an immune response against the immunogenic protein; and a pharmaceutically acceptable vaccine carrier.

The invention discloses a method for carrying out protein chip detection on brucellosis, and relates to the technical field of brucellosis detection. The preparation method comprises the following steps: spotting a Brucella synergistic composite antigen for multi-index joint detection on a pretreated solid-phase carrier, drying and washing; then adding a sealing agent for sealing, and washing to obtain a protein chip; then collecting an initial sample, diluting and centrifuging, and taking supernate; adding the supernate to a protein chip site, oscillating, incubating, washing, adding a secondary antibody, further incubating and washing; and finally, reading the signal by using a fluorescence scanner, deducting the background, and interpreting the positive and negative properties. The method is wide in detection range, strong in specificity and high in sensitivity, and has a very wide application prospect.

In one aspect, an EID button tag is provided herein for tagging and identifying cattle as being inoculated against brucellosis, the EID button tag including: a shell having a central opening; a transponder located in the shell; a male punch having a disc-shaped punch body and a stem extending distally therefrom, the stem having a head configured for irreversible insertion into the central opening, wherein a mounting opening is formed through the punch body with a mounting channel extending distally from the mounting opening and coaxially along the stem; and, a vaccination identification button having a disc-shaped visual identification body and a mounting stem extending distally therefrom, wherein the mounting stem is configured for irreversible insertion into the mounting channel. The punch body and the visual identification body are primarily tinted with different colors to provide visual identification of a head of cattle being inoculated against brucellosis.

This invention provides a live attenuated Brucella vaccine strain M5Δ pyrE Its construction methods and applications belong to the field of biotechnology. The M5... pyrE The strain was obtained by knocking out Brucella mesenteriae strain M5. pyrE Genetically constructed and deposited at the China Center for Type Culture Collection. This invention discovers that... pyrE The gene is associated with Brucella virulence; knocking out this gene significantly weakens Brucella virulence and reduces its intracellular and mouse viability. Compared to the existing vaccine strain M5-9026, M5... pyrE The strain exhibits superior immunoprotective effects, effectively inducing humoral and Th1 cellular immune responses, and demonstrates good safety. The M5 strain constructed in this invention... pyrE This strain has promising potential as a candidate strain for a live attenuated vaccine to prevent brucellosis in animals.

This invention discloses a primer set, kit, and method for detecting Brucella, belonging to the field of gene detection technology. The primer set comprises two pairs of specific RPA primers: primer pairs B1F1 (SEQ ID No. 3) and B1R1 (SEQ ID No. 6) targeting the B1 sequence, and primer pairs B2F1 (SEQ ID No. 8) and B2R1 (SEQ ID No. 11) targeting the B2 sequence. This invention also provides a kit containing this primer set and a detection method based on RPA-CRISPR-Cas12a. This application combines the high efficiency of RPA amplification with the ultra-high specificity of CRISPR-Cas12a detection, overcoming the shortcomings of traditional methods such as long processing time, low sensitivity, and susceptibility to false positives. It achieves rapid, sensitive, and specific detection of Brucella, and has significant application value in the early screening and monitoring of brucellosis.

The invention provides a feed additive for preventing sheep brucellosis and a preparation method thereof, and belongs to the technical field of animal feed additives. The feed additive for preventing sheep brucellosis is prepared from the following components in parts by weight: 25-35 parts of radix scutellariae, 18-24 parts of herba houttuyniae, 15-20 parts of herba andrographitis, 12-16 parts of herba portulacae, 10-15 parts of radix angelicae sinensis, 10-15 parts of colla corii asini, 5-10 parts of rhizoma atractylodis macrocephalae, 4-8 parts of coix seeds and 3-5 parts of radix glycyrrhizae. The components of the feed additive are used together, so that the feed additive can inhibit the growth and reproduction of brucella, regulate the gastrointestinal health of animals, improve the immunity and enhance the disease resistance of the animals, thereby effectively preventing and controlling sheep brucellosis. The feed additive disclosed by the invention is good in prevention and treatment effect, small in toxic and side effects and not easy to generate drug resistance.

The invention relates to the technical field of molecular marker assisted breeding, and discloses an SNP molecular marker related to a sheep brucellosis resistance character and a combination and application thereof. The SNP molecular marker combination is composed of 12 SNP molecular markers which are obviously associated with the sheep brucellosis resistance character. The SNP molecular markers provided by the invention are clear in position, and a molecular probe combination, a gene chip and a kit developed by utilizing the 12 SNP molecular markers can realize early prediction of the brucellosis resistance character of sheep, and can more accurately detect the brucellosis resistance of the sheep; the molecular marker is used for breeding of anti-brucellosis sheep and molecular marker-assisted breeding of the anti-brucellosis property of the sheep, and the breeding efficiency of the anti-brucellosis sheep is effectively improved.

The invention discloses a method for preparing a sheep-derived positive serum reference of yersinia enterocolitica, obtained positive serum and application of the positive serum, and belongs to the field of veterinary medicine products. The invention aims to solve the technical problem of how to obtain the yersinia enterocolitica sheep-derived positive serum reference which is stable in titer and character and can be used for specific detection of brucella diagnostic products. Therefore, the invention provides a method for preparing the yersinia enterocolitica sheep-derived positive serum reference, which comprises the following steps: immunizing a tested sheep by using an immunizing antigen, and collecting serum of the immunized tested sheep to obtain the yersinia enterocolitica sheep-derived positive serum reference. The immune antigen is an inactivated yersinia enterocolitica obtained by inactivating the yersinia enterocolitica. The positive serum reference can be effectively applied to quality control evaluation work of brucellosis related diagnosis products, and plays a positive role in prevention and control of brucellosis.

The invention relates to the technical field of antibody titer digitization analysis, in particular to a brucellosis antibody titer digitization analysis system based on a spectrophotometric method.According to the brucellosis antibody titer digitization analysis system, a full-time reaction kinetics absorbance curve is obtained through a data acquisition module; a signal decomposition module is utilized to decouple the signal into a low-frequency trend component and a high-frequency detail component; further identifying a reaction kinetics phase state through a low-frequency trend analysis module to lock an effective reaction interval and generate first-dimension analysis data, and controlling a high-frequency detail analysis module to generate second-dimension analysis data by taking the first-dimension analysis data as a time domain gating basis; finally, the comprehensive evaluation module constructs a coupling weight model based on the reaction kinetics phase state, and fuses the two-dimensional data to generate a comprehensive antibody activity evaluation value; according to the process, a self-adaptive analysis mechanism conforming to a reaction evolution rule is constructed, and accurate determination of the titer of the antibody is realized.

Provided herein are methods, kits and systems for the detection of Brucella antigen, method for detecting brucellosis in a mammal comprising the steps of: obtaining or having obtained a biological sample suspected being infected with Brucella sp.; and detecting in the biological sample immunogenic protein DUF883 or anti-DUF883 antibodies, wherein the presence of DUF883 or anti-DUF883 antibodies are indicative that the mammal has been exposed to Brucella, e.g., to a vaccine or immunogenic composition comprising: immunogenic protein DUF883 provided in an amount sufficient to trigger an immune response against the immunogenic protein; and a pharmaceutically acceptable vaccine carrier.

The application discloses an ELISA antibody detection kit for identifying Brucella gene deletion live vaccine (M5-90△26 strain) and wild strains and application. The kit comprises enzyme-linked reaction plate 1 and enzyme-linked reaction plate 2. The enzyme-linked reaction plate 1 is coated with LPS extracted from the Brucella gene deletion live vaccine (M5-90△26 strain), and the enzyme-linked reaction plate 2 is coated with a Brucella BP26 antigen epitope polypeptide combination composed of polypeptides with amino acid sequences shown in SEQ ID NO:1-2. The application is suitable for goats and sheep inoculated with the Brucella gene deletion live vaccine (M5-90△26 strain), can effectively distinguish the antibody produced by the Brucella gene deletion live vaccine (M5-90△26 strain) and the antibody produced by wild strain infection, thereby realizing identification and detection, being favorable for prevention and control of brucellosis and optimization of a vaccination strategy, and providing technical support for healthy and sustainable development of animal husbandry.

The present invention provides a classification model for sheep echinococcosis and sheep brucellosis based on serum Raman spectroscopy, a method for establishing the model, and a detection device. The method for establishing the classification model for sheep echinococcosis and sheep brucellosis based on serum Raman spectroscopy comprises: (1) collecting blood from healthy sheep and diseased sheep, extracting serum, and obtaining serum samples; (2) detecting the serum samples using a laser Raman spectrometer to obtain Raman spectral data; (3) performing noise reduction, baseline correction, and normalization on the Raman spectral data to obtain pre-processed spectral data; (4) performing principal component analysis (PCA) dimensionality reduction on the processed spectral data, and then establishing a linear discriminant analysis (LDA) or support vector machine (SVM) classification model to obtain a classification model for sheep echinococcosis and sheep brucellosis. The classification model and detection device established by the present invention based on machine learning algorithms and Raman spectroscopy have potential in screening sheep infected with echinococcosis and sheep infected with brucellosis.

The Brucellosis Huhong plate agglutination test device comprises a reaction substrate and a stirring comb. The reaction substrate is integrally molded from a milky opaque plastic, has a reaction front surface and a support back surface, in which an array of blind holes is formed, each blind hole defining a circular opening in the reaction front surface of the reaction substrate and defining an inner circumferential wall and a circular bottom in a thickness direction of the reaction substrate, wherein the diameter of the inner circumferential wall of each blind hole is gradually reduced from the circular opening to the circular bottom, the diameter of the circular opening is 19-21 mm, and the diameter of the circular bottom is 15-17 mm. The stirring comb is provided with at least one row of parallel stirring needles, and the stirring needles correspond to at least one row of blind holes in the blind hole array respectively so that the stirring needles can stretch into the blind holes in a stirring mode. According to the Brucellosis Tianhong plate agglutination test device disclosed by the utility model, multiple samples can be detected at the same time, the mutual pollution and interference among the samples are effectively avoided, and meanwhile, the samples are ensured to have enough flowability within the test specified result interpretation time, so that the accurate detection can be carried out.

The present invention provides a Brucellosis cell immune protein and use thereof. In the present invention, firstly, a Brucellosis cell immune protein with relatively high immunogenicity is screened by means of antibody spectrum technology; then, the protein gene sequence of Brucella are subjected to fusion expression by using molecular biology technology, so as to improve the ability for producing an immune response which is induced by an antigen; next, the antigen is expressed in vitro and then subjected to multi-stage purification, and the obtained purified protein is used as a stimulant; and finally, anti-coagulated blood from an immunized animal is collected, the stimulant is added thereto, the mixture is incubated for 24 hours at 37° C., the resulting product is centrifuged to collect a supernatant, and the level of IL-17 in the supernatant is detected by an ELISA method.

The invention provides a specific polypeptide of Brucella, the amino acid sequence of the specific polypeptide is as shown in SEQ ID No.1, and the specific polypeptide is derived from BMEI1871 protein of Brucella. The invention also provides a recombinant vector or an expression cassette for expressing the specific polypeptide, and a recombinant cell or a recombinant bacterium. The invention verifies the application of the specific polypeptide in preparation of drugs or reagents for preventing, detecting or diagnosing brucellosis, and also provides an ELISA (enzyme-linked immuno sorbent assay) detection kit for detecting brucellosis. According to the present invention, the specific polypeptide of the brucella BMEI1871 protein is adopted as the antigen to establish the ELISA method for detecting the brucella antibody, such that the accuracy is high, and the powerful support is provided for brucellosis purification. The antigen source is convenient, the stability is high, the repeatability is strong, and the defects of using whole bacteria or lipopolysaccharide as the antigen are overcome.

The invention belongs to the technical field of biological medicines, and particularly relates to a lactic acid bacteria live vector vaccine for preventing and treating brucellosis and a preparation method of the lactic acid bacteria live vector vaccine. The lactic acid bacteria live vector vaccine is obtained by transferring expression vectors for expressing Brucella omp19 protein and BvrR protein into lactic acid bacteria, and the lactic acid bacteria are lactobacillus cavings. The lactobacillus live vector vaccine for preventing and treating brucellosis is constructed by taking brucella omp19 protein and BvrR protein as immunogens and lactic acid bacteria as an antigen transfer vector, and the safety is good. After the vaccine is used for immunizing mice, the mice can obtain a higher antibody level, the duration is long, brucella infection is effectively inhibited, meanwhile, the production process of the vaccine is simplified, and the vaccine has a good application prospect.

The invention provides a VjbR protein purification method and application thereof, and relates to the technical field of protein purification. According to the purification method of the VjbR protein provided by the invention, a reducing agent and a surfactant with specific concentration are added into an equilibrium buffer solution and an elution buffer solution of Ni affinity chromatography, and a surfactant with specific concentration is added into an equilibrium buffer solution and an elution buffer solution of ion exchange chromatography, so that the purity and the yield of the VjbR protein are effectively improved; and the purified VjbR protein has stable properties, is not easy to precipitate, can be stored for a long time, and provides a basis for the development of a brucellosis diagnosis technology.

The present application relates to the field of animal breeding technology, and particularly relates to a molecular marker related to sheep brucellosis susceptibility and application thereof.The molecular marker comprises nucleic acid of a nucleotide sequence as shown in SEQ ID NO.1, and polymorphism exists at the 101st position, and the polymorphism is G / A.A molecular marker related to sheep brucellosis susceptibility is screened by the present application, and the susceptibility of sheep brucellosis can be identified by detecting the polymorphism of the molecular marker.The molecular marker provided by the present application can also be used for breeding sheep varieties with low brucellosis susceptibility, which has important significance in the field of sheep breeding.

The invention discloses an accurate dose control method and system for brucellosis traditional Chinese and western medicine combined medication, and relates to the technical field of medical treatment. According to the method, individualized data of a patient is obtained through a multi-modal data acquisition technology, a population pharmacokinetic-pharmacodynamic model is constructed by using the data, the population pharmacokinetic-pharmacodynamic model comprises a western medicine module, a traditional Chinese medicine module and a coupling interface, and pharmacokinetic modeling of traditional Chinese medicine and western medicine combined medication is achieved. By monitoring the physiological indexes and symptom data of a patient in real time and combining a traditional Chinese medicine syndrome dynamic scoring algorithm and a reinforcement learning dynamic dose adjustment algorithm, the real-time accurate adjustment of the drug dose is realized. In addition, the method further comprises a traditional Chinese and western medicine synergistic effect optimization step, a synergistic index is calculated based on the knowledge graph and the graph attention network, and a dosage optimization rule is formulated, and the implementation of the method is beneficial for improving the treatment effect of brucellosis and reducing side effects.

The present application relates to the field of animal breeding technology, and particularly relates to a molecular marker and application thereof in identifying sheep brucellosis susceptibility. The molecular marker comprises nucleic acid of a nucleotide sequence shown as SEQ ID NO. 1, and a polymorphism exists at the 101th position, and the polymorphism is C / G. The present application identifies a molecular marker related to sheep brucellosis susceptibility. By analyzing the genetic polymorphism of the marker, the susceptibility of sheep individuals to brucellosis can be determined. In addition, the molecular marker can also be used as an effective auxiliary selection tool, and is applied to breeding of new sheep strains with stronger brucellosis resistance, which has important application value for disease-resistant breeding of sheep.

The present invention provides a monoclonal cell line 2D3 that specifically secretes BP26 antibodies, a single-chain antibody, and a preparation method, belonging to the field of veterinary biotechnology. The present invention expresses and purifies the Brucella canis BP26 protein. The spleen cells of mice immunized with the protein are fused with SP2 / 0 cells in vitro to obtain the monoclonal cell line 2D3 that specifically secretes BP26 antibodies. Subsequently, RNA of the monoclonal cell 2D3 is extracted, reverse transcribed into cDNA, and the V sequence expressing the monoclonal antibody is amplified. L and V H , and V L and V H Gene ligation was performed to obtain a single-chain antibody gene. This gene was ligated to the pCold-TF vector and transformed into BL21 competent cells for induced expression, yielding a single-chain antibody against the Brucella canis BP26 protein. The single-chain antibody against the Brucella canis BP26 protein of the present invention can bind to the BP26 protein and is of great significance for the development of new brucellosis detection reagents.

The present invention relates to a brucellosis rose bengal analyzer, which includes a mounting base, a push plate mechanism, a liquid transfer mechanism, an oscillation mechanism and an interpretation mechanism. The mounting base is provided with a sample area, a reaction area, a recovery area and a pre-storage area. The push plate mechanism is arranged in the pre-storage area and includes a push plate, which can push the reaction plate in the pre-storage area to the reaction area and push the reaction plate in the reaction area to the recovery area. The liquid transfer mechanism is arranged above the mounting base, the oscillation mechanism is arranged in the reaction area and below the reaction plate, and the interpretation mechanism is arranged above the mounting base. The brucellosis rose bengal analyzer has a high degree of automation in the detection process, saves manual time, reduces labor costs, has high detection efficiency, and reduces personnel infection at the same time.

The present invention provides a gntR17 gene deleted strain M5ΔgntR17 strain and its construction method and application, belonging to the field of biotechnology. The M5ΔgntR17 strain is deposited in the General Microbiology Center of China Committee for the Collection of Microorganisms, with a deposit date of July 4, 2024, and a deposit number of CGMCCNO.46061. The present invention found that the gntR17 gene is a related gene that affects the virulence of Brucella, and the deletion of the gntR17 gene can weaken the virulence of Brucella and improve safety. After deletion, it can significantly reduce the survival ability of Brucella in cells and mice, and has potential application value. The M5ΔgntR17 strain constructed after knocking out the gntR17 gene has weakened virulence than the original Brucella M5 strain, has high safety, and has similar immune protection to the M5-90Δ26 vaccine, and can be used as a candidate live attenuated vaccine strain for preventing animal brucellosis.