118 results about "Brucellosis" patented technology

A sheep single nucleotide polymorphism (SNP) molecular marker and use thereof in detecting resistance to brucellosis in sheep are provided, relating to the technical field of molecular marker-assisted breeding. A sheep SNP molecular marker is provided, where the sheep SNP molecular marker is associated with resistance to brucellosis in sheep, and has a nucleotide sequence shown in SEQ ID NO: 1 in which a T / C polymorphism is located at 101st bp. The sheep SNP molecular marker can accurately detect a resistance level of sheep to the brucellosis, and is used for the selection of brucellosis-resistant sheep as well as molecular marker-assisted breeding of the resistance to brucellosis in sheep. The sheep SNP molecular marker effectively improves a breeding efficiency of sheep with brucellosis resistance.

The invention relates to the technical field of space-time prediction, in particular to a brucellosis space-time prediction method and system based on artificial intelligence, and the method comprises the following steps: obtaining case information, calculating an incidence relation, extracting a path sequence, screening a trend direction, and matching a new case to generate prediction data. According to the method, continuous identification of a propagation path is realized by constructing a propagation incidence relation between cases and introducing a spatial directivity index, a non-trend propagation process is screened out through an angle average value and a variance, spatial consistency of path screening is enhanced, and through joint matching of spatio-temporal characteristics of newly-added cases and an existing propagation trend, the propagation path screening efficiency is improved. The sensitivity of prediction to the trend attribution of a new case is improved, the spatial directivity of a potential disease area is enhanced through reverse projection of a trend path and area positioning drop point frequency analysis, and through linkage processing of multi-level path extraction, trend judgment and area coding, the probability of occurrence of the new case is lowered. And the capturing capability of the prediction data on the propagation and evolution characteristics of the brucellosis is improved.

The invention discloses a primer group, a kit and a method for detecting brucella, and belongs to the technical field of gene detection. The primer group comprises two pairs of specific RPA (recombinase polymerase amplification) primers: a primer pair B1F1 (SEQ ID No.3) and a primer pair B1R1 (SEQ ID No.6) targeting a B1 sequence, and a primer pair B2F1 (SEQ ID No.8) and a primer pair B2R1 (SEQ ID No.11) targeting a B2 sequence. The invention also provides a kit containing the primer group, and a detection method based on the RPA-CRISPR-Cas12a. The invention also provides a kit containing the primer group and a detection method based on the RPA-CRISPR-Cas12a. According to the application, the high efficiency of RPA amplification is combined with the ultrahigh specificity of CRISPR-Cas12a detection, the defects of long time consumption, low sensitivity and easy generation of false positive in the traditional method are overcome, rapid, sensitive and specific detection of Brucella is realized, and the application has important application value in early screening and monitoring of Brucella.

The invention relates to a PCR (Polymerase Chain Reaction) kit and method for identifying brucella pathogens and application. The PCR kit comprises primer pairs and probes for simultaneously detecting brucella BA0711 vaccine strains, M5-9026 vaccine strains, Rev.1 vaccine strains and wild strains. A primer pair for detecting the brucella BA0711 vaccine strain is shown as SEQ ID NO.1-2, and a probe is shown as SEQ ID NO.3; a primer pair for detecting the M5-9026 vaccine strain is shown as SEQ ID NO.4-5, and a probe is shown as SEQ ID NO.6; a primer pair for detecting the Rev.1 vaccine strain is shown as SEQ ID NO.7-8, and a probe is shown as SEQ ID NO.9; a primer pair for detecting positive brucella is shown as SEQ ID NO.10-11, and a probe is shown as SEQ ID NO.12; the kit has the advantages of high sensitivity and strong specificity, and can simultaneously distinguish a plurality of vaccine strains and wild strains.

The invention relates to the technical field of risk monitoring, in particular to a brucellosis risk monitoring and early warning method based on remote sensing and GIS (Geographic Information System), which comprises the following steps of: acquiring multi-stage remote sensing images to construct an ecological grid set, hanging cases, positioning and generating a disease source boundary map layer, identifying a high vegetation and temperature intersection area to form a risk structure, and carrying out early warning on the risk structure. And extracting the display area and superposing the GIS map to output early warning information. According to the method, the multi-stage remote sensing image covering the key epidemic area is obtained, region segmentation and geocoding registration are executed, by means of coupling detection of the normalized vegetation index and the land surface temperature, the spatial intersection region with the pathogen propagation suitable ecological condition can be identified, the risk index is calculated according to the graph block structure distribution, and the risk level label is given; according to the method, accurate positioning and hierarchical evaluation of a high-risk ecological environment and historical case space overlapping region are realized, the space prediction precision and the intuition of risk prompt are improved, and the capability of responding to the brucellosis potential outbreak trend in advance is effectively enhanced.

The invention discloses a penicillium fungus GS218, a penicillium fungus GS218 conidium suspension and a preparation method and application of the penicillium fungus GS218 conidium suspension. The penicillium fungus GS218 is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC M 20241477, and the penicillium fungus GS218 is separated from fruits of trifoliate orange in a Hunan Institute of Gardening Institute of Hunan Province in the East Lake Street, Hibiscus District, Changsha City, Hunan Province. The penicillium fungus has a relatively good antagonistic effect on HLB13 fusarium buchneri, colletotrichum spinosus, fusarium solani, fusarium oxysporum cucumber specialized type and botrytis cinerea; the composition can be used for effectively preventing and treating carrot diseases caused by HLB13 fusarium brucellosis, pepper anthracnose caused by colletotrichum spinosus, pepper root rot caused by fusarium solani, cucumber fusarium wilt caused by fusarium oxysporum cucumber specialization and botrytis cinerea caused by botrytis cinerea, and has remarkable and stable technical effects; the method has wide application value in the technical field of microbial strain application.

The present invention belongs to the field of biomedicine technology, and specifically relates to the use of a Brucella OMP16 monoclonal antibody in the preparation of a product for inhibiting Brucella, wherein the heavy chain variable region amino acid sequence of the Brucella OMP16 monoclonal antibody is shown in SEQ ID NO.1, and the light chain variable region amino acid sequence is shown in SEQ ID NO.2. Through in vivo and in vitro tests, the present invention verifies the potential of the OMP16 monoclonal antibody as a therapeutic antibody in the treatment of brucellosis.

The invention provides a gntR17 gene deleted strain M5 delta gntR17 as well as a construction method and application thereof, and belongs to the technical field of biology. The M5 delta gntR17 strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation date is July 4, 2024, and the preservation number is CGMCC NO.46061. The M5 delta gntR17 strain is preserved in the China General Microbiological Culture Collection Center (CGMCC). It is found that the gntR17 gene is a related gene affecting the virulence of the brucella, the virulence of the brucella can be weakened by deletion of the gntR17 gene, the safety is improved, the survival ability of the brucella in cells and a mouse body can be remarkably reduced after deletion of the gntR17 gene, and the gntR17 gene has potential application value. Compared with the original brucella M5 strain, the M5 delta gntR17 strain constructed after the gntR17 gene is knocked out has the advantages that the virulence is weakened, the safety is high, the immune protection similar to that of an M5-90 delta 26 vaccine is realized, and the strain can be used as a candidate attenuated live vaccine strain for preventing animal brucellosis.

The invention relates to a silicon-based nano material for targeted therapy of brucellosis as well as a preparation method and application of the silicon-based nano material. The invention relates to a preparation method of a silicon-based nano material for targeted therapy of brucellosis. The preparation method comprises the following steps: (1) preparing magnetic mesoporous silica; (2) aminating the magnetic mesoporous silica, and modifying the magnetic mesoporous silica with a responsive material and yeast beta-glucan to obtain modified magnetic mesoporous silica; and (3) carrying out antibiotic loading on the modified magnetic mesoporous silica. According to the silicon-based nano material for targeted therapy of brucellosis as well as the preparation method and the application of the silicon-based nano material, the surface of magnetic macroporous mesoporous silica is modified with yeast beta-glucan capable of targeting M cells and glutathione responsive molecules, and a drug delivery system sensitive to a brucellosis environment is synthesized; the nano-carrier has targeted targeting selectivity for delivering and releasing drugs, the activity, slow release and target cell uptake efficiency of antibiotics are ensured, and the brucellosis treatment is more accurate and efficient.

The invention relates to a colloidal gold test strip for detecting a sheep pox virus antibody as well as a preparation method and application of the colloidal gold test strip. According to the invention, firstly, a recombinant 122 protein is prepared, and the recombinant 122 protein has remarkable antigenicity and immunogenicity and can be used as a good target for diagnosing the sheep pox virus; secondly, the colloidal gold test strip for detecting the sheep pox virus antibody is prepared, a sample pad, a gold-labeled pad, a nitrocellulose membrane and a water absorption pad are sequentially arranged on a PVC base plate of the test strip, and the gold-labeled pad is coated with gold-labeled recombinant 122 protein; the test strip can simultaneously detect antibodies of the sheep pox virus, the bovine nodular skin disease virus and the goat pox virus, the sensitivity can reach 1: 128, 1: 128 and 1: 64 respectively, the test strip has no cross reaction with bovine viral diarrhea virus, foot-and-mouth disease O-type virus, foot-and-mouth disease A-type virus, orf virus, brucellosis and colibacillosis, the specificity is good, and the diagnosis coincidence rate reaches 93%.

The invention relates to the technical field of markers, in particular to application of a biomarker for identifying acute stage and chronic stage of brucellosis. A series of biomarkers related to acute and chronic stages of brucellosis are screened and found, and the biomarkers have obvious difference between acute and chronic brucellosis patients. The biomarkers can realize acute and chronic staging of brucellosis, are high in sensitivity and specificity, and have relatively good diagnosis efficiency. A novel molecular diagnosis tool is provided for acute and chronic staging of brucellosis, the problems that existing serological detection is low in specificity, and clinical staging cannot be accurately judged are solved, and wide application prospects are achieved.

The invention is applicable to the technical field of genetic engineering and biological medicine, and provides construction of a fusion recombinant protein ABT for preventing brucellosis and application of the fusion recombinant protein ABT in preparation of protective vaccines. The fusion recombinant protein ABT is composed of an immunological enhancement antigen and a multi-epitope tandem antigen, wherein the immunological enhancement antigen (named as A) comprises a Brucella ribosome L7 / L12 protein and a PADRE sequence; the multi-epitope tandem antigen is formed by connecting a dominant B cell epitope part (named as B), a dominant Tc cell epitope part and a dominant Th cell epitope part (named as T) in series, wherein the dominant B cell epitope part (named as B) is derived from Brucella SurA, OMP31, BP26 and Trigger factor proteins. The ABT Brucella multi-epitope subunit vaccine prepared by mixing the fusion recombinant protein ABT and an immunologic adjuvant has the characteristics of good purity, high safety and strong immunogenicity, can stimulate an organism to generate a protective antibody, and has long antibody maintenance time.

The invention discloses a preparation method of manganese dioxide nano-enzyme and application of the manganese dioxide nano-enzyme in a disease detection kit.The preparation method of the manganese dioxide nano-enzyme comprises the following steps that a fish peptone solution containing fish peptone powder and a potassium permanganate aqueous solution are mixed to be uniform, a mixed solution is obtained, the mixed solution is stirred to be brown, and a manganese dioxide nano-enzyme solution is obtained; the manganese dioxide nano-enzyme has an ultra-small particle size of 2-5nm, overcomes the problem that the traditional nano-enzyme is small in particle size and easy to agglomerate, and has excellent dispersity and stability; in addition, the manganese dioxide nano-enzyme prepared from the fish peptone powder has high peroxidase-like activity and stability, and the enzyme activity of the manganese dioxide nano-enzyme is 2.36 times higher than that of HRP in a brucellosis detection ELISA kit.

Provided herein are methods and compositions for the detection of Brucella antigen, and more particularly, to a vaccine or immunogenic composition comprising: Brucella strain from which at least a portion of the immunogenic protein DUF883 has been deleted provided in an amount sufficient to trigger an immune response against the immunogenic protein; and a pharmaceutically acceptable vaccine carrier.

The invention discloses a method for carrying out protein chip detection on brucellosis, and relates to the technical field of brucellosis detection. The preparation method comprises the following steps: spotting a Brucella synergistic composite antigen for multi-index joint detection on a pretreated solid-phase carrier, drying and washing; then adding a sealing agent for sealing, and washing to obtain a protein chip; then collecting an initial sample, diluting and centrifuging, and taking supernate; adding the supernate to a protein chip site, oscillating, incubating, washing, adding a secondary antibody, further incubating and washing; and finally, reading the signal by using a fluorescence scanner, deducting the background, and interpreting the positive and negative properties. The method is wide in detection range, strong in specificity and high in sensitivity, and has a very wide application prospect.

In one aspect, an EID button tag is provided herein for tagging and identifying cattle as being inoculated against brucellosis, the EID button tag including: a shell having a central opening; a transponder located in the shell; a male punch having a disc-shaped punch body and a stem extending distally therefrom, the stem having a head configured for irreversible insertion into the central opening, wherein a mounting opening is formed through the punch body with a mounting channel extending distally from the mounting opening and coaxially along the stem; and, a vaccination identification button having a disc-shaped visual identification body and a mounting stem extending distally therefrom, wherein the mounting stem is configured for irreversible insertion into the mounting channel. The punch body and the visual identification body are primarily tinted with different colors to provide visual identification of a head of cattle being inoculated against brucellosis.

The embodiment of the present invention provides a Mongolian medicine and Mongolian medicine preparation for treating brucellosis. The Mongolian medicine is composed of the following raw materials in parts by weight: 3-7 parts of long-haired silver anemone, 3-6 parts of terminalia chebula, 3-6 parts of donkey ear wind chrysanthemum, 2-4 parts of multi-leaf thorn beans, 2-4 parts of purple flower aconite, 2-4 parts of horn fennel, 2-4 parts of aconite leaves, 2-4 parts of liquorice, 2-4 parts of coptis root, 2-4 parts of scutellaria, 2-4 parts of borneol, 2-4 parts of sophora flavescens, 2-4 parts of cyrtomium, 2-4 parts of calamus, 2-4 parts of processed aconite, 2-4 parts of bamboo yellow, 2-4 parts of purple ginseng, 2-4 parts of black borneol, 2-4 parts of scutellaria baicalensis, 2-4 parts of musk. The Mongolian medicine provided by the present invention is a traditional prescription, and it can be seen from a large number of clinical trials that the Mongolian medicine of the present invention has a definite curative effect on brucellosis.

This invention provides a live attenuated Brucella vaccine strain M5Δ pyrE Its construction methods and applications belong to the field of biotechnology. The M5... pyrE The strain was obtained by knocking out Brucella mesenteriae strain M5. pyrE Genetically constructed and deposited at the China Center for Type Culture Collection. This invention discovers that... pyrE The gene is associated with Brucella virulence; knocking out this gene significantly weakens Brucella virulence and reduces its intracellular and mouse viability. Compared to the existing vaccine strain M5-9026, M5... pyrE The strain exhibits superior immunoprotective effects, effectively inducing humoral and Th1 cellular immune responses, and demonstrates good safety. The M5 strain constructed in this invention... pyrE This strain has promising potential as a candidate strain for a live attenuated vaccine to prevent brucellosis in animals.

The present invention discloses a monoclonal antibody cell strain 6F6 for camel immunoglobulin G and its application. The 6F6 strain has been deposited in the General Microbiology Center CGMCC of the China Microbiological Culture Collection Administration Committee, and its address is the Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing. It is classified and named as a monoclonal antibody cell strain of camel immunoglobulin G (IgG), with a deposit date of August 9, 2023, and a deposit number of CGMCC No.45650. Through artificial expression, purification, immunization and screening of camel IgG protein, a monoclonal antibody cell strain 6F6 that can stably and efficiently express camel IgG was obtained. It was identified as the IgG1 subtype, and the recognition epitope was KEPPSMRLKARPG. It has been successfully applied to the antibody detection of camel brucellosis, and the established method has good sensitivity and specificity.

The utility model provides a full-automatic brucellosis antibody detection workstation. The full-automatic brucellosis antibody detection workstation comprises a cabinet body, a pipettor, a sample frame, a sample tube, a suction head box, an oscillation module, an experiment board, a waste liquid tank, a photographing unit, a disinfectant tank and a reagent tank, the suction head box, the waste liquid tank, the disinfectant tank and the reagent tank are located in the middle, and the suction head box is used for providing suction head consumables; the waste liquid tank is used for recycling waste liquid and sucker consumables, the disinfectant tank is used for providing disinfectant, and the reagent tank is used for providing reagents. According to the multi-region physical sample loading device disclosed by the utility model, sample loading, accurate pipetting, liquid transferring, pore plate filling, reagent loading, uniform mixing and oscillation, reagent extraction, detection system construction and sample photographing can be integrated, and multi-region physical division can be carried out according to the sample loading requirements of an experiment sequence, time and each step; cross contamination of samples is avoided, the high-throughput sample treatment requirement is met, full oscillation and uniform mixing can be realized, the accuracy of a detection result is improved, and the detection efficiency is also greatly improved.

This invention discloses a primer set, kit, and method for detecting Brucella, belonging to the field of gene detection technology. The primer set comprises two pairs of specific RPA primers: primer pairs B1F1 (SEQ ID No. 3) and B1R1 (SEQ ID No. 6) targeting the B1 sequence, and primer pairs B2F1 (SEQ ID No. 8) and B2R1 (SEQ ID No. 11) targeting the B2 sequence. This invention also provides a kit containing this primer set and a detection method based on RPA-CRISPR-Cas12a. This application combines the high efficiency of RPA amplification with the ultra-high specificity of CRISPR-Cas12a detection, overcoming the shortcomings of traditional methods such as long processing time, low sensitivity, and susceptibility to false positives. It achieves rapid, sensitive, and specific detection of Brucella, and has significant application value in the early screening and monitoring of brucellosis.

The invention provides a feed additive for preventing sheep brucellosis and a preparation method thereof, and belongs to the technical field of animal feed additives. The feed additive for preventing sheep brucellosis is prepared from the following components in parts by weight: 25-35 parts of radix scutellariae, 18-24 parts of herba houttuyniae, 15-20 parts of herba andrographitis, 12-16 parts of herba portulacae, 10-15 parts of radix angelicae sinensis, 10-15 parts of colla corii asini, 5-10 parts of rhizoma atractylodis macrocephalae, 4-8 parts of coix seeds and 3-5 parts of radix glycyrrhizae. The components of the feed additive are used together, so that the feed additive can inhibit the growth and reproduction of brucella, regulate the gastrointestinal health of animals, improve the immunity and enhance the disease resistance of the animals, thereby effectively preventing and controlling sheep brucellosis. The feed additive disclosed by the invention is good in prevention and treatment effect, small in toxic and side effects and not easy to generate drug resistance.

The invention relates to the technical field of molecular marker assisted breeding, and discloses an SNP molecular marker related to a sheep brucellosis resistance character and a combination and application thereof. The SNP molecular marker combination is composed of 12 SNP molecular markers which are obviously associated with the sheep brucellosis resistance character. The SNP molecular markers provided by the invention are clear in position, and a molecular probe combination, a gene chip and a kit developed by utilizing the 12 SNP molecular markers can realize early prediction of the brucellosis resistance character of sheep, and can more accurately detect the brucellosis resistance of the sheep; the molecular marker is used for breeding of anti-brucellosis sheep and molecular marker-assisted breeding of the anti-brucellosis property of the sheep, and the breeding efficiency of the anti-brucellosis sheep is effectively improved.

The invention discloses a method for preparing a sheep-derived positive serum reference of yersinia enterocolitica, obtained positive serum and application of the positive serum, and belongs to the field of veterinary medicine products. The invention aims to solve the technical problem of how to obtain the yersinia enterocolitica sheep-derived positive serum reference which is stable in titer and character and can be used for specific detection of brucella diagnostic products. Therefore, the invention provides a method for preparing the yersinia enterocolitica sheep-derived positive serum reference, which comprises the following steps: immunizing a tested sheep by using an immunizing antigen, and collecting serum of the immunized tested sheep to obtain the yersinia enterocolitica sheep-derived positive serum reference. The immune antigen is an inactivated yersinia enterocolitica obtained by inactivating the yersinia enterocolitica. The positive serum reference can be effectively applied to quality control evaluation work of brucellosis related diagnosis products, and plays a positive role in prevention and control of brucellosis.

The invention relates to the technical field of antibody titer digitization analysis, in particular to a brucellosis antibody titer digitization analysis system based on a spectrophotometric method.According to the brucellosis antibody titer digitization analysis system, a full-time reaction kinetics absorbance curve is obtained through a data acquisition module; a signal decomposition module is utilized to decouple the signal into a low-frequency trend component and a high-frequency detail component; further identifying a reaction kinetics phase state through a low-frequency trend analysis module to lock an effective reaction interval and generate first-dimension analysis data, and controlling a high-frequency detail analysis module to generate second-dimension analysis data by taking the first-dimension analysis data as a time domain gating basis; finally, the comprehensive evaluation module constructs a coupling weight model based on the reaction kinetics phase state, and fuses the two-dimensional data to generate a comprehensive antibody activity evaluation value; according to the process, a self-adaptive analysis mechanism conforming to a reaction evolution rule is constructed, and accurate determination of the titer of the antibody is realized.

The utility model provides a multi-layer kit for brucellosis detection, which belongs to the technical field of brucellosis detection and comprises a kit body and a kit cover, movable notches are arranged at four corners of the upper surface of the kit body, telescopic fixers are arranged at the bottoms of the movable notches, telescopic connectors are arranged at four corners of the lower surface of the kit cover, and the telescopic connectors correspond to the movable notches. A sealing plate is arranged in the middle of the lower surface of the box cover, the length and width of the sealing plate are consistent with those of the top of the kit body, the sealing plate is in sealing fit with the top of the kit body, and the telescopic connector is in sealing fit with the movable notch. A buckle and a clamping groove are connected in a clamped mode through a positioning soft plate, meanwhile, the top of the kit body is sealed through a sealing plate, the first reagent bottle and the standby reagent bottle are prevented from falling off, the kit body is convenient to carry by hand through a portable handle, and a hanging ring can be hung on the waist of a worker and is convenient to carry.

Provided herein are methods, kits and systems for the detection of Brucella antigen, method for detecting brucellosis in a mammal comprising the steps of: obtaining or having obtained a biological sample suspected being infected with Brucella sp.; and detecting in the biological sample immunogenic protein DUF883 or anti-DUF883 antibodies, wherein the presence of DUF883 or anti-DUF883 antibodies are indicative that the mammal has been exposed to Brucella, e.g., to a vaccine or immunogenic composition comprising: immunogenic protein DUF883 provided in an amount sufficient to trigger an immune response against the immunogenic protein; and a pharmaceutically acceptable vaccine carrier.

The application discloses an ELISA antibody detection kit for identifying Brucella gene deletion live vaccine (M5-90△26 strain) and wild strains and application. The kit comprises enzyme-linked reaction plate 1 and enzyme-linked reaction plate 2. The enzyme-linked reaction plate 1 is coated with LPS extracted from the Brucella gene deletion live vaccine (M5-90△26 strain), and the enzyme-linked reaction plate 2 is coated with a Brucella BP26 antigen epitope polypeptide combination composed of polypeptides with amino acid sequences shown in SEQ ID NO:1-2. The application is suitable for goats and sheep inoculated with the Brucella gene deletion live vaccine (M5-90△26 strain), can effectively distinguish the antibody produced by the Brucella gene deletion live vaccine (M5-90△26 strain) and the antibody produced by wild strain infection, thereby realizing identification and detection, being favorable for prevention and control of brucellosis and optimization of a vaccination strategy, and providing technical support for healthy and sustainable development of animal husbandry.

The invention discloses a kit for simultaneously detecting border disease virus, brucellosis, toxoplasmosis, sheep endemic abortion and goat arthritis virus, and belongs to the technical field of virus detection. The kit comprises primers as shown in SEQ ID NO. 1 to SEQ ID NO. 18 and a probe as shown in SEQ ID NO. 18. According to the invention, after a single system of border disease virus, brucellosis, toxoplasmosis, sheep endemic abortion and goat arthritis virus is tested, a sextuple system containing an internal standard is established, and through tests of indexes such as linearity, minimum detection limit, specificity, precision and repeatability, NTC repetition, comparison of multiple fluorescent RT-PCR and single fluorescent RT-PCR, the kit can be used for detecting the sheep endemic abortion and the goat arthritis. The currently developed fluorescent RT-PCR multiple detection kit for the sheep reproductive disorder syndrome completely meets the development requirement, and the lowest detection limit can reach 10 Copis / mu L or below.

The present invention provides a classification model for sheep echinococcosis and sheep brucellosis based on serum Raman spectroscopy, a method for establishing the model, and a detection device. The method for establishing the classification model for sheep echinococcosis and sheep brucellosis based on serum Raman spectroscopy comprises: (1) collecting blood from healthy sheep and diseased sheep, extracting serum, and obtaining serum samples; (2) detecting the serum samples using a laser Raman spectrometer to obtain Raman spectral data; (3) performing noise reduction, baseline correction, and normalization on the Raman spectral data to obtain pre-processed spectral data; (4) performing principal component analysis (PCA) dimensionality reduction on the processed spectral data, and then establishing a linear discriminant analysis (LDA) or support vector machine (SVM) classification model to obtain a classification model for sheep echinococcosis and sheep brucellosis. The classification model and detection device established by the present invention based on machine learning algorithms and Raman spectroscopy have potential in screening sheep infected with echinococcosis and sheep infected with brucellosis.