247 results about "Transfer vector" patented technology

The present invention relates to modified nucleic acid sequences coding for coagulation factor VIII (FVIII) and for von Willebrand factor (VWF) as well as complexes thereof and their derivatives, recombinant expression vectors containing such nucleic acid sequences, host cells transformed with such recombinant expression vectors, recombinant polypeptides and derivatives coded for by said nucleic acid sequences which recombinant polypeptides and derivatives do have biological activities together with prolonged in vivo half-life and / or improved in vivo recovery compared to the unmodified wild-type protein. The invention also relates to corresponding FVIII sequences that result in improved expression yield. The present invention further relates to processes for the manufacture of such recombinant proteins and their derivatives. The invention also relates to a transfer vector for use in human gene therapy, which comprises such modified nucleic acid sequences.

The present invention relates to a gene transfer vector (GTV) and in particular to an integrating gene transfer vector (IGTV), which comprises at least one genetic insulator element (GIE), wherein the each comprises at least two copies of an element selected from the group consisting of: a CTF binding site; a first CTCF binding site and a second CTCF binding site, wherein the first and the second CTCF binding sites are derived from the regulatory sequences of different genes.

The present application discloses a lentiviral transfer system which includes: (i) a self-inactivating transfer vector comprising: multiple gene units, wherein each gene unit includes a heterologous nucleic acid sequence operably linked to a regulatory nucleic acid sequence; and (ii) a helper construct which lacks a 5′ LTR, wherein the 5′ LTR has been replaced with a heterologous promoter, in which the helper construct further comprises: a lentiviral env nucleic acid sequence containing a deletion, wherein the deleted env nucleic acid sequence does not produce functional env protein; and a packaging signal contains a deletion, wherein the deleted packaging signal is nonfunctional.

An architecture is shown where an execution unit is tightly coupled to a shared, reconfigurable memory system. Sequence control signals drive a DMA controller and address generator to control the transfer of data from the shared memory to a bus interface unit (BIU). The sequence control signals also drive a data controller and address generator which controls transfer of data from the shared memory to an execution unit interface (EUI). The EUI is connected to the execution unit operates under control of the data controller and address generator to transfer vector data to and from the shared memory. The shared memory is configured to swap memory space in between the BIU and the execution unit so as to support continuous execution and I / O. A local fast memory is coupled to the execution unit. A local address generator controls the transfer of scalar data between the local fast memory and the execution unit. The execution unit, local fast memory and local address generator form a fast memory path that is not dependent upon the slower data path between the execution unit and shared memory. The fast memory path provides for fast execution of scalar operations in the execution unit and rapid state storage and retrieval for operations in the execution unit.