5 results about "Genetic code" patented technology

This invention discloses a method for preparing and applying plant sulfonated small peptide PSK based on synthetic biology, belonging to the field of biotechnology. Based on the design concept of synthetic biology, this invention constructs an orthogonal synthetic system composed of genetic code expansion technology and SUMO fusion protease digestion technology. A specific PSK dotted vector and a pUltra-sY vector are co-transformed into *E. coli* to construct a genetically engineered strain. The sulfonated small peptide PSK precursor is expressed in this genetically engineered strain, and mature plant sulfonated small peptide PSK is obtained through specific hydrolysis by SUMO protease. This method significantly improves the synthesis efficiency and purity of sulfonated small peptides and has broad application prospects in agricultural production and biotechnology.

The invention relates to a computer-implemented method performed by one or more data processing apparatus for assessing a biological feature of a biological macromolecule, preferably a protein molecule, by providing an electrostatic data based in silico representation of the biological macromolecule. The biological feature may be, without limitation, multiple function-related states of the macromolecule, differences in the conformation, e.g. in the secondary structure, patterns based on primary structure, folded nature of the protein including, among others, π-π relationships and relationship between the genetic code and the electrostatic state of the environment of the encoded amino acid(s). The invention can be used among others in the field of drug research, medical diagnosis, genetic engineering and macromolecular modeling.

The present application relates to the construction and application of the EcORep-based continuous directed evolution reporter system of aminoacyl-tRNA synthetase, and specifically, the reporter system comprises: a green fluorescent protein mutant sfGFP containing amber stop codon TAG N150TAG ; a chloramphenicol acetyltransferase mutant CAT containing amber stop codon TAG D112TAG ; a linear replicon containing aminoacyl-tRNA synthetase; a reporter plasmid containing a plus-strand mismatch DNA polymerase to continuously mutate the linear replicon, thereby generating an in-vivo library of aminoacyl-tRNA synthetase mutants; and a tRNA, which is orthogonal to the aminoacyl-tRNA synthetase and enables the incorporation of unnatural amino acids and the complete expression of proteins. The present application proves that the evolution reporter system can realize the efficient directed evolution of aminoacyl-tRNA synthetase, and further expands the application range of genetic code expansion technology.

This invention discloses a method for site-specific fluorescent labeling of proteins in cell lysates and its applications, belonging to the field of pharmaceutical bioengineering technology. Utilizing genetic code amplification technology, non-natural amino acids containing azide groups are introduced at specific sites of the target protein. A long-lifetime terbium complex modified with dibenzocyclooctylene is directly added to the cell lysate, enabling copper-free click chemistry labeling of the target protein. This method exhibits good biocompatibility and wide applicability, and can construct CoraFluor-DBCO-labeled protein target libraries. Furthermore, a time-resolved fluorescence resonance energy transfer (TR-FRET) screening system is constructed, utilizing the long fluorescence lifetime of the terbium complex to filter out biological background fluorescence and improve the signal-to-noise ratio. Competitive TR-FRET enables homogeneous screening of small molecule ligands targeting the target protein in the lysate. This method avoids protein purification steps, is simple to operate, and has high screening efficiency.