21 results about "Genetic code" patented technology

This invention discloses a method for preparing and applying plant sulfonated small peptide PSK based on synthetic biology, belonging to the field of biotechnology. Based on the design concept of synthetic biology, this invention constructs an orthogonal synthetic system composed of genetic code expansion technology and SUMO fusion protease digestion technology. A specific PSK dotted vector and a pUltra-sY vector are co-transformed into *E. coli* to construct a genetically engineered strain. The sulfonated small peptide PSK precursor is expressed in this genetically engineered strain, and mature plant sulfonated small peptide PSK is obtained through specific hydrolysis by SUMO protease. This method significantly improves the synthesis efficiency and purity of sulfonated small peptides and has broad application prospects in agricultural production and biotechnology.

The Quadruplet Expanded DNA (QED) eukaryote genetic code comprising twenty nondegenerate QED codons encode proteins (the protein-encoding codons), and thirty-five nondegenerate QED codons (the noncoding codons) being highly correlated with cis-regulatory elements control and regulate transcription, alternate splicing, and polymerization in eukaryotic protein synthesis using canonical amino acids. The QED eukaryote genetic code is an advancement to gene therapeutics that allows for the correction of dysfunctional proteins. Additionally, the QED eukaryote genetic code is further applicable for changing paradigms relating to identifying cures for monogenic rare, multigene cancer, and neurodegenerative diseases.

The invention relates to a computer-implemented method performed by one or more data processing apparatus for assessing a biological feature of a biological macromolecule, preferably a protein molecule, by providing an electrostatic data based in silico representation of the biological macromolecule. The biological feature may be, without limitation, multiple function-related states of the macromolecule, differences in the conformation, e.g. in the secondary structure, patterns based on primary structure, folded nature of the protein including, among others, π-π relationships and relationship between the genetic code and the electrostatic state of the environment of the encoded amino acid(s). The invention can be used among others in the field of drug research, medical diagnosis, genetic engineering and macromolecular modeling.

The invention discloses a method for constructing a vascularized tumor microenvironment based on a genetic code recoding technology, which comprises the following steps of: constructing biological orthogonal translation systems PiggyBac-(7sk-tRNAPyl) x4-EF1a-MmPylRS and PiggyBac-ETV2122 *, transfecting the biological orthogonal translation systems and transposase plasmids into a liver cancer cell line, and screening a Huh-7 cell line which stably expresses the biological orthogonal translation systems; and carrying out balling culture on the obtained Huh-7 cell line, and adding NAEK in the culture process to induce partial Huh-7 cells to be transdifferentiated into endothelial cells, so as to obtain vascularized liver cancer cell balls and realize the construction of a tumor microenvironment. The genetic code recoding technology is used for regulating and controlling formation of the functional vascular tumor microenvironment, and the method has the advantages of being simple and easy to operate and high in repeatability and universality. The technology is a near-physiological protein translation regulation and control means, the regulation and control speed is high, protein translation regulation and control can be rapidly started or stopped, a traditional two-dimensional culture mode can be replaced, multi-cell interaction is achieved, and the biomimetic property is better.

The invention relates to a sandy soil remodeling gene compiling method and system driven by dynamic characteristics, and the method comprises the steps: obtaining a small strain shear modulus of indoor and outdoor sandy soil through the density of the sandy soil, and obtaining a normalized small strain shear modulus after correction through an overlying stress correction formula; indoor and outdoor test results are integrated to obtain an indoor-in-situ fusion data set, so that an artificial neural network is trained, and optimal variable state parameters of indoor remolded sand are searched through an intelligent optimization algorithm; and taking an absolute value of a difference between a predicted value of the artificial neural network and a corresponding outdoor normalized small strain shear modulus as an optimization objective function, carrying out iterative optimization to establish an indoor-in-situ intelligent remodeling model, and generating an optimal variable state parameter and a corresponding sandy soil gene. The problem of dynamic characteristic deviation caused by the fact that a traditional sandy soil remodeling technology depends on static parameters is solved, and high-precision equivalence of indoor and outdoor soil dynamic response is achieved.

Provided are cells that are resistant to mobile genetic elements or horizontal gene transfer, and methods for obtaining said cells. Also provided are methods for preventing the horizontal transfer of genetic information between a mobile genetic element and a first cell, cells making use of new genetic codons schemes and related subject matter, kits comprising mutually orthogonal cells, and mobile genetic elements. Also provided are methods of altering the susceptibility of a gene to mutations that alter the encoded amino acid sequence, methods for evolving or improving a protein, and methods for rendering a target gene more resistant to mutation. Additionally provided are uses of the cells for making polymers and methods comprising using the cells for making polymers.

The present application relates to the construction and application of the EcORep-based continuous directed evolution reporter system of aminoacyl-tRNA synthetase, and specifically, the reporter system comprises: a green fluorescent protein mutant sfGFP containing amber stop codon TAG N150TAG ; a chloramphenicol acetyltransferase mutant CAT containing amber stop codon TAG D112TAG ; a linear replicon containing aminoacyl-tRNA synthetase; a reporter plasmid containing a plus-strand mismatch DNA polymerase to continuously mutate the linear replicon, thereby generating an in-vivo library of aminoacyl-tRNA synthetase mutants; and a tRNA, which is orthogonal to the aminoacyl-tRNA synthetase and enables the incorporation of unnatural amino acids and the complete expression of proteins. The present application proves that the evolution reporter system can realize the efficient directed evolution of aminoacyl-tRNA synthetase, and further expands the application range of genetic code expansion technology.

The present invention relates to novel bifunctional molecules capable of undergoing bioorthogonal reactions and inducing proximity between two proteins, methods of inducing proximity between two proteins employing said novel molecules and bioorthogonal reactions and genetic code expansion, methods of evaluating protein-protein proximity interactions employing the novel bifunctional molecules, medical uses of the novel molecules, and methods of treatment of a disease involving post-translational modifications of a protein employing the novel bifunctional molecules.

The invention provides an assembled and annotated chloroplast genome of euphorbia splendens, and relates to the fields of molecular biology and plant genetics. The chloroplast genome of the pteris amurensis is a single cyclic molecule; the total length of the chloroplast genome of the psidium aquilinum is 149180bp; the nucleotide sequence of the chloroplast genome of the pteris amurensis is as shown in SEQ ID NO. 1; the GC content of the chloroplast genome of the pteris amurensis is 43.14%; the pteridium aquilinum chloroplast genome provided by the invention enriches a pteridium gene database, lays a foundation for exploring pteridium aquilinum evolution history and an adaptation mechanism of pteridium aquilinum to environmental stress, decrypts genetic codes of pteridium aquilinum from a molecular level, and is applied to biodiversity protection, plant ecological restoration and gardening.

The present application relates to a kind of genetic code NAD+ protein probe based on resonance energy transfer and its preparation method and application, specifically disclose a kind of genetic code NAD+ protein probe, it is formed by resonance energy transfer donor, NAD+ response protein and resonance energy transfer acceptor series connection;Wherein NAD+ response protein is the mutant of DNA ligase, the sequence of the mutant of DNA ligase is as shown in SEQ ID NO.3, or SEQ ID NO.6;The resonance energy transfer donor is selected from luciferase or fluorescent protein;The resonance energy transfer acceptor is selected from fluorescent protein and the fluorescent protein as resonance energy transfer acceptor is different from the fluorescent protein of resonance energy transfer donor.The protein probe of the present application can be synthesized in living cell and be used to detect NAD+ concentration in living cell.

The invention relates to the field of biosensing, in particular to a genetic coding fluorescent probe for detecting lactic acid change in autophagosome and application of the genetic coding fluorescent probe. The invention discloses a genetic coding fluorescent probe for detecting lactic acid change in an autophagy body, which comprises an FRET pair, a lactic acid sensing structural domain and an autophagy body targeting structural domain and is used for specifically detecting the lactic acid concentration change in the autophagy body in real time, when the lactic acid concentration in the autophagy body changes, the conformation of the probe changes, and the change of the lactic acid concentration in the autophagy body changes. As a result, the fluorescence resonance energy transfer efficiency changes, and dynamic, non-invasive and high-temporal-spatial-resolution detection of the concentration of the lactic acid in the autophagy body can be realized by monitoring the change of the FRET ratio.

This invention discloses a method for site-specific fluorescent labeling of proteins in cell lysates and its applications, belonging to the field of pharmaceutical bioengineering technology. Utilizing genetic code amplification technology, non-natural amino acids containing azide groups are introduced at specific sites of the target protein. A long-lifetime terbium complex modified with dibenzocyclooctylene is directly added to the cell lysate, enabling copper-free click chemistry labeling of the target protein. This method exhibits good biocompatibility and wide applicability, and can construct CoraFluor-DBCO-labeled protein target libraries. Furthermore, a time-resolved fluorescence resonance energy transfer (TR-FRET) screening system is constructed, utilizing the long fluorescence lifetime of the terbium complex to filter out biological background fluorescence and improve the signal-to-noise ratio. Competitive TR-FRET enables homogeneous screening of small molecule ligands targeting the target protein in the lysate. This method avoids protein purification steps, is simple to operate, and has high screening efficiency.

This invention relates to the field of data processing technology and discloses a digital operation and maintenance management method and system for the entire life cycle of ultra-high voltage insulators. The method includes: acquiring the digital genetic code of the insulator upon leaving the factory and anchoring it on the blockchain; simultaneously collecting dynamic phenotypic characteristics and micro-meteorological parameters related to grid connection; splicing and fusing the underlying heterogeneous data tensors, calculating edge weights based on wind direction and distance attenuation rules, and constructing a dynamic spatiotemporal map base; using a time-series graph convolutional network to perform deep evolutionary inference on the map, outputting a quantitative degradation risk score; dynamically generating differentiated operation and maintenance strategies and underlying control instructions based on this, and mapping virtual storage capacity demand parameters through trend extrapolation; identifying common defects, and issuing process optimization instructions to the manufacturing source after strict tamper-proof verification using blockchain. This invention completely breaks down cross-domain data silos, realizing a closed-loop leap from passive emergency repairs to predictive operation and maintenance and pre-scheduled supply chain scheduling for insulators.

The present invention relates to novel, improved aminoacyl-tRNA synthetase variants, which are useful for genetic code expansion. The present invention also relates to corresponding coding sequences and eukaryotic cell lines comprising such coding sequences. The present invention also relates to methods of preparing a protein of interest (POI) comprising one or more unnatural amino acid residues incorporated by means of the novel aminoacyl-tRNA synthetase variants. The present invention also relates to a method of preparing polypeptide conjugates, wherein a POI generated by means of the present aminoacyl-tRNA synthetase variants is reacted with one or more conjugation partner molecules. Finally, the present invention relates to a kit comprising the required constituents for preparing such POIs comprising one or more unnatural amino acid residues.

The invention relates to a genetic coding pyruvic acid fluorescent probe capable of locating a subcellular structure, a preparation method of the genetic coding pyruvic acid fluorescent probe and application of the genetic coding pyruvic acid fluorescent probe in macrophages. The probe comprises a pyruvic acid sensitive recognition structural domain, a fluorescence signal report structural domain and a subcellular localization signal peptide which can perform targeted expression in a specific subcellular structure of macrophages, so that real-time monitoring and quantitative analysis of the concentration of pyruvic acid are realized. The probe provided by the invention has the advantages of strong specificity, high sensitivity, rapid response and the like, and is suitable for cell metabolism dynamic research, power-assisted metabolism mechanism analysis and drug target discovery. In the aspect of disease research, abnormal metabolism of macrophages is closely related to myocardial infarction and the like, the malic enzyme 2 is regulated and controlled to improve the pyruvic acid level, improve the polarization state of the macrophages and enhance the anti-inflammatory and tissue repair functions of the macrophages, and the potential protection effect on ischemic heart tissue is shown. The probe disclosed by the invention is expected to be used as an important tool for researching macrophage metabolism regulation and related disease intervention means.

The invention discloses a method for specific covalent coupling of a monoclonal antibody drug by using a Z structural domain, which comprises the following steps: introducing non-natural amino acid fluorosulfonic acid-L-tyrosine (FSY) capable of being covalently cross-linked at a specific site of the Z structural domain by a genetic code expansion technology, and converting non-covalent interaction between the Z structural domain and an IgG monoclonal antibody drug into covalent interaction; the binding force of the two molecules is enhanced, the stability is good under the physiological condition, stable and irreversible binding is realized, and the stability and uniformity of the coupled molecules can be remarkably improved. The method can be used as an XDC (antibody coupling medicine, antibody coupling probe, antibody coupling diagnostic reagent and the like) platform method, and is compatible with various loads such as medicine molecules, imaging molecules, nucleic acid modification and the like.

The invention discloses non-natural amino acid inserted ferritin as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The recombinant ferritin 5F-HFn is efficiently prepared by inserting an unnatural amino acid 4-azido-phenylalanine containing an azido group into the 5th site in a natural amino acid sequence of the human heavy-chain ferritin HFn through a genetic code expansion technology based on termination codon inhibition and combining a double-plasmid transformation and nickel column purification process. A copper-free click reaction is utilized to realize directional coupling with a dibenzocyclooctyne modified DNA fragment or other functional molecules, so that the nano-carrier is endowed with multifunctional characteristics of targeted delivery, biological imaging and the like, and a nano-carrier platform capable of being flexibly configured is constructed.

A fully genetically encoded NAD+protein probe based on resonance energy transfer, and a preparation method therefor and the use thereof. Specifically provided is a fully genetically encoded NAD+protein probe, which is formed by means of linking a resonance energy transfer donor, an NAD+responsive protein and a resonance energy transfer receptor in series, wherein the NAD+ responsive protein is a mutant of a DNA ligase; the sequence of the mutant of the DNA ligase is as shown in SEQ ID NO.3 or SEQ ID NO.6; the resonance energy transfer donor is selected from luciferase or a fluorescent protein; the resonance energy transfer receptor is selected from a fluorescent protein; and the fluorescent protein as the resonance energy transfer receptor is different from that of the resonance energy transfer donor. The protein probe can be synthesized in living cells and used for detecting the concentration of NAD+ in living cells.

The invention discloses a fluorescent fixed-point labeling method for protein in a cell lysis solution and application, and belongs to the technical field of medical bioengineering. The method comprises the following steps: introducing non-natural amino acid containing an azide group into a specific site of a target protein by using a genetic code expansion technology, directly adding a dibenzocyclooctyne modified terbium complex with long fluorescence lifetime into a cell lysis solution, and carrying out fixed-point labeling on the target protein through copper-free click chemistry. The method disclosed by the invention is good in biocompatibility and wide in applicability, and can be used for constructing a protein target library marked by the CoFluor-DBCO. A time-resolved fluorescence resonance energy transfer screening system is further constructed, biological background fluorescence is filtered out by utilizing the long fluorescence lifetime of the terbium complex, and the signal-to-noise ratio is increased. By adopting the competitive TR-FRET, the homogeneous screening of the small molecule ligand of the targeted target protein in the lysis solution can be realized, the protein purification step is avoided, the operation is simple and convenient, and the screening efficiency is high.

The application discloses a kind of encryption communication method and system based on biological genetic code chaos driving, comprising: receiving original bit stream and initial key, generates chaos sequence group according to initial key using two hyperchaos model;Original bit stream is encrypted using chaos sequence DNA coding to obtain gene transmission sequence, and transmission bit stream is obtained by DNA decoding to gene transmission sequence;Transmission bit stream is mapped to constellation to obtain constellation diagram, and constellation diagram is scrambled according to chaos sequence sub-carrier, symbol scrambling and constellation rotation;Transmission signal is obtained by the inverse discrete Fresnel transform matrix to constellation diagram OCDM modulation;Initial key is hidden in the frame header of transmission signal, and transmission signal is sent to receiver by transmitter;The application realizes the multilayer encryption of OCDM signal and dynamic key transmission.

To incorporate novel substrates into sequence defined polymers.SOLUTION: Disclosed are methods, systems, components and compositions for synthesis of sequence defined polymers. The methods, systems, components and compositions may be utilized for incorporating novel substrates that include non-standard amino acid monomers and non-amino acid monomers into sequence defined polymers. As disclosed herein, the novel substrates may be utilized for acylation of tRNA via flexizyme catalyzed reactions. The tRNAs thus acylated with the novel substrates may be utilized in synthesis platforms for incorporating the novel substrates into a sequence defined polymer.SELECTED DRAWING: Figure 1A