34 results about "Genetic code" patented technology

The invention discloses an encryption communication method and system based on biological genetic coding chaos driving, and the method comprises the steps: receiving an original bit stream and an initial secret key, and generating a chaos sequence group according to the initial secret key through two hyperchaos models; performing DNA coding encryption on the original bit stream by using the chaos sequence to obtain a gene transmission sequence, and performing DNA decoding on the gene transmission sequence to obtain a transmission bit stream; performing constellation mapping on the transmission bit stream to obtain a constellation diagram, and performing subcarrier scrambling, symbol scrambling and constellation rotation on the constellation diagram according to the chaos sequence; performing OCDM modulation on the constellation diagram through an inverse discrete Fresnel transformation matrix to obtain a transmission signal; hiding the initial key in a frame header of a transmission signal, and sending the transmission signal to a receiver through a transmitter; according to the invention, multi-layer encryption of the OCDM signal and dynamic secret key follow-up transmission are realized.

This invention discloses a method for preparing and applying plant sulfonated small peptide PSK based on synthetic biology, belonging to the field of biotechnology. Based on the design concept of synthetic biology, this invention constructs an orthogonal synthetic system composed of genetic code expansion technology and SUMO fusion protease digestion technology. A specific PSK dotted vector and a pUltra-sY vector are co-transformed into *E. coli* to construct a genetically engineered strain. The sulfonated small peptide PSK precursor is expressed in this genetically engineered strain, and mature plant sulfonated small peptide PSK is obtained through specific hydrolysis by SUMO protease. This method significantly improves the synthesis efficiency and purity of sulfonated small peptides and has broad application prospects in agricultural production and biotechnology.

The Quadruplet Expanded DNA (QED) eukaryote genetic code comprising twenty nondegenerate QED codons encode proteins (the protein-encoding codons), and thirty-five nondegenerate QED codons (the noncoding codons) being highly correlated with cis-regulatory elements control and regulate transcription, alternate splicing, and polymerization in eukaryotic protein synthesis using canonical amino acids. The QED eukaryote genetic code is an advancement to gene therapeutics that allows for the correction of dysfunctional proteins. Additionally, the QED eukaryote genetic code is further applicable for changing paradigms relating to identifying cures for monogenic rare, multigene cancer, and neurodegenerative diseases.

The invention relates to a computer-implemented method performed by one or more data processing apparatus for assessing a biological feature of a biological macromolecule, preferably a protein molecule, by providing an electrostatic data based in silico representation of the biological macromolecule. The biological feature may be, without limitation, multiple function-related states of the macromolecule, differences in the conformation, e.g. in the secondary structure, patterns based on primary structure, folded nature of the protein including, among others, π-π relationships and relationship between the genetic code and the electrostatic state of the environment of the encoded amino acid(s). The invention can be used among others in the field of drug research, medical diagnosis, genetic engineering and macromolecular modeling.

The invention belongs to the field of biotechnology, and specifically relates to a method for introducing an early stop codon at a specific amino acid residue of a foot-and-mouth disease virus VP1 protein based on a genetic codon expansion technology, and using a biological orthogonal translation system to read through an amber stop codon UAG. Under the condition of the presence or absence of a non-natural amino acid, not only can the expression of the VP1 protein be conditionally controlled, and the non-natural amino acid can be introduced into the foot-and-mouth disease virus VP1 protein at a specific point, but also the foot-and-mouth disease virus VP1 protein can be specifically labeled with a non-natural amino acid containing a special group, and a stable covalent bond can be formed between proteins that interact with cells through light cross-linking, thereby providing a powerful means for studying the interaction between the foot-and-mouth disease virus and host proteins; and the method can also be used to prepare a replication-deficient recombinant foot-and-mouth disease virus and a vaccine with a mutation at a specific site of the VP1 protein.

Mobile genetic elements or cells that are resistant to horizontal gene transfer, and methods for obtaining the cells, are provided. Also provided are methods for suppressing horizontal transfer of genetic information between a mobile genetic element and a first cell, cells utilizing novel gene codon schemes and related subject matter, and kits containing cells and mobile genetic elements that are orthogonal to one another. Also provided are methods for altering the susceptibility of a gene to mutations that alter the encoded amino acid sequence, methods for evolving or improving proteins, and methods for making a target gene more resistant to mutations. Additionally, the use of cells to make polymers and methods comprising using cells to make polymers are provided.

The present invention relates to the technical field of functional enzymes, and in particular to an α-L-fucosidase mutant and an application thereof. The amino acid sequence of the mutant has at least 80% homology to the amino acid sequence of fucosidase SEQ ID NO: 1; the mutant has at least one characteristic sequence of the fucosidase, and the amino acid sequence of the fucosidase is modified, substituted, deleted or added with one or more amino acids to obtain the amino acid sequence of the mutant; the mutant has an amino acid sequence encoded by a nucleotide sequence shown in SEQ ID NO: 2 or its complementary sequence, or a sequence that is different from the nucleotide sequence of the nucleotide sequence shown in SEQ ID NO: 2 or its complementary sequence due to the degeneracy of the genetic code. The mutant provided by the present invention is beneficial to reducing the cost and reaction time in the catalytic reaction of fucosylated compound synthesis.

The invention discloses a method for constructing a vascularized tumor microenvironment based on a genetic code recoding technology, which comprises the following steps of: constructing biological orthogonal translation systems PiggyBac-(7sk-tRNAPyl) x4-EF1a-MmPylRS and PiggyBac-ETV2122 *, transfecting the biological orthogonal translation systems and transposase plasmids into a liver cancer cell line, and screening a Huh-7 cell line which stably expresses the biological orthogonal translation systems; and carrying out balling culture on the obtained Huh-7 cell line, and adding NAEK in the culture process to induce partial Huh-7 cells to be transdifferentiated into endothelial cells, so as to obtain vascularized liver cancer cell balls and realize the construction of a tumor microenvironment. The genetic code recoding technology is used for regulating and controlling formation of the functional vascular tumor microenvironment, and the method has the advantages of being simple and easy to operate and high in repeatability and universality. The technology is a near-physiological protein translation regulation and control means, the regulation and control speed is high, protein translation regulation and control can be rapidly started or stopped, a traditional two-dimensional culture mode can be replaced, multi-cell interaction is achieved, and the biomimetic property is better.

To provide orthogonal translation systems which allow for the site-specific introduction of non-canonical amino acid residues into a target protein (POI).SOLUTION: The present invention relates to assembler fusion proteins, which bring an RNA-targeting polypeptide (RNA-TP) segment and an orthogonal aminoacyl tRNA synthetase (O-RS) segment into spatial proximity of one another, either by direct linkage in RNA-TP / O-RS fusion proteins, or though the action of "assemblers" fused to each of these segments in assembler fusion proteins (AFPs). The invention also relates to AFP combinations and nucleic acid molecules comprising a POI-encoding sequence together with a targeting nucleotide sequence that is able to interact with an RNA-TP. The invention further relates to nucleic acid molecules, expression cassettes and expression vectors and the like encoding the RNA-TP / O-RS fusion proteins or AFPs.SELECTED DRAWING: None

The invention relates to a sandy soil remodeling gene compiling method and system driven by dynamic characteristics, and the method comprises the steps: obtaining a small strain shear modulus of indoor and outdoor sandy soil through the density of the sandy soil, and obtaining a normalized small strain shear modulus after correction through an overlying stress correction formula; indoor and outdoor test results are integrated to obtain an indoor-in-situ fusion data set, so that an artificial neural network is trained, and optimal variable state parameters of indoor remolded sand are searched through an intelligent optimization algorithm; and taking an absolute value of a difference between a predicted value of the artificial neural network and a corresponding outdoor normalized small strain shear modulus as an optimization objective function, carrying out iterative optimization to establish an indoor-in-situ intelligent remodeling model, and generating an optimal variable state parameter and a corresponding sandy soil gene. The problem of dynamic characteristic deviation caused by the fact that a traditional sandy soil remodeling technology depends on static parameters is solved, and high-precision equivalence of indoor and outdoor soil dynamic response is achieved.

Provided are cells that are resistant to mobile genetic elements or horizontal gene transfer, and methods for obtaining said cells. Also provided are methods for preventing the horizontal transfer of genetic information between a mobile genetic element and a first cell, cells making use of new genetic codons schemes and related subject matter, kits comprising mutually orthogonal cells, and mobile genetic elements. Also provided are methods of altering the susceptibility of a gene to mutations that alter the encoded amino acid sequence, methods for evolving or improving a protein, and methods for rendering a target gene more resistant to mutation. Additionally provided are uses of the cells for making polymers and methods comprising using the cells for making polymers.

Cells resistant to moving genetic elements or horizontal gene transfer, as well as methods for obtaining the cells, are provided. Also provided are methods for preventing horizontal transfer of genetic information between a mobile genetic element and a first cell, cells utilizing the new genetic codon regimen and related subject matter, kits comprising mutually orthogonal cells, and mobile genetic elements. Also provided are methods of altering the susceptibility of a gene to altering mutations of an encoded amino acid sequence, methods for evolving or improving a protein, and methods for making a target gene more resistant to mutations. Also provided are uses of the cells for making polymers and methods comprising making polymers using the cells.

The present application relates to the construction and application of the EcORep-based continuous directed evolution reporter system of aminoacyl-tRNA synthetase, and specifically, the reporter system comprises: a green fluorescent protein mutant sfGFP containing amber stop codon TAG N150TAG ; a chloramphenicol acetyltransferase mutant CAT containing amber stop codon TAG D112TAG ; a linear replicon containing aminoacyl-tRNA synthetase; a reporter plasmid containing a plus-strand mismatch DNA polymerase to continuously mutate the linear replicon, thereby generating an in-vivo library of aminoacyl-tRNA synthetase mutants; and a tRNA, which is orthogonal to the aminoacyl-tRNA synthetase and enables the incorporation of unnatural amino acids and the complete expression of proteins. The present application proves that the evolution reporter system can realize the efficient directed evolution of aminoacyl-tRNA synthetase, and further expands the application range of genetic code expansion technology.

The present invention relates to novel bifunctional molecules capable of undergoing bioorthogonal reactions and inducing proximity between two proteins, methods of inducing proximity between two proteins employing said novel molecules and bioorthogonal reactions and genetic code expansion, methods of evaluating protein-protein proximity interactions employing the novel bifunctional molecules, medical uses of the novel molecules, and methods of treatment of a disease involving post-translational modifications of a protein employing the novel bifunctional molecules.

The invention provides an assembled and annotated chloroplast genome of euphorbia splendens, and relates to the fields of molecular biology and plant genetics. The chloroplast genome of the pteris amurensis is a single cyclic molecule; the total length of the chloroplast genome of the psidium aquilinum is 149180bp; the nucleotide sequence of the chloroplast genome of the pteris amurensis is as shown in SEQ ID NO. 1; the GC content of the chloroplast genome of the pteris amurensis is 43.14%; the pteridium aquilinum chloroplast genome provided by the invention enriches a pteridium gene database, lays a foundation for exploring pteridium aquilinum evolution history and an adaptation mechanism of pteridium aquilinum to environmental stress, decrypts genetic codes of pteridium aquilinum from a molecular level, and is applied to biodiversity protection, plant ecological restoration and gardening.

The invention provides nuclear localization signal sequence construction for improving tumor cell radiotherapy sensitivity through a nuclear PTEN path and application thereof, and relates to the technical field of biology. The nuclear localization signal sequence construction method for improving the radiotherapy sensitivity of the tumor cells through the nuclear PTEN way specifically comprises the following steps: firstly, extracting total RNA (Ribonucleic Acid) of the cells according to a Trizol kit specification, quantifying the RNA, then searching a human wild type PTEN gene sequence according to NCBI-genebank, selecting a PMD-T vector and pcDNA3.1 as gene vectors, and designing an N-terminal nuclear localization signal according to a genetic code; carrying out RT-PCR (Reverse Transcription-Polymerase Chain Reaction) by using a primer 1 and a primer 2 according to extracted RNA (Ribonucleic Acid) as a template, and after a PCR product is subjected to 1% agarose gel electrophoresis, showing a specific band at about 1200bp; the PTEN gene fragment is subjected to gel cutting and recovery, and then is connected with a PMD-18T vector to construct the recombinant PMD-PTEN. PTEN low-expression tumor cells are transfected through nuclear PTEN, the tumor radiotherapy sensitivity is improved, and huge benefits can be brought to tumor patients.

The present invention provides a plate cutting and typesetting method based on order combination optimization, comprising the following steps: obtaining plate information of an order to be scheduled and performing information splitting; obtaining a set of characteristic points and a set of line segment points of each plate; performing gene encoding on each plate; initializing the first-generation target population; according to the gene order, typeset the transformation graphics of each plate on the original material sheet, and obtain the typesetting scheme of all plates on the original material sheet; scoring each individual of the contemporary target population by genetic algorithm; introducing the gray wolf elite strategy and the gray wolf expulsion strategy in the process of population variation; obtaining the optimal individual based on the genetic algorithm score; and determining the typesetting scheme of all plates on the original material sheet according to the optimal individual. The method takes maximizing the utilization of the original material sheet as the basic goal, and can improve the combination arrangement optimization capability in the plate cutting process, save production costs and improve production efficiency.

The present application relates to a kind of genetic code NAD+ protein probe based on resonance energy transfer and its preparation method and application, specifically disclose a kind of genetic code NAD+ protein probe, it is formed by resonance energy transfer donor, NAD+ response protein and resonance energy transfer acceptor series connection;Wherein NAD+ response protein is the mutant of DNA ligase, the sequence of the mutant of DNA ligase is as shown in SEQ ID NO.3, or SEQ ID NO.6;The resonance energy transfer donor is selected from luciferase or fluorescent protein;The resonance energy transfer acceptor is selected from fluorescent protein and the fluorescent protein as resonance energy transfer acceptor is different from the fluorescent protein of resonance energy transfer donor.The protein probe of the present application can be synthesized in living cell and be used to detect NAD+ concentration in living cell.

The invention relates to the field of biosensing, in particular to a genetic coding fluorescent probe for detecting lactic acid change in autophagosome and application of the genetic coding fluorescent probe. The invention discloses a genetic coding fluorescent probe for detecting lactic acid change in an autophagy body, which comprises an FRET pair, a lactic acid sensing structural domain and an autophagy body targeting structural domain and is used for specifically detecting the lactic acid concentration change in the autophagy body in real time, when the lactic acid concentration in the autophagy body changes, the conformation of the probe changes, and the change of the lactic acid concentration in the autophagy body changes. As a result, the fluorescence resonance energy transfer efficiency changes, and dynamic, non-invasive and high-temporal-spatial-resolution detection of the concentration of the lactic acid in the autophagy body can be realized by monitoring the change of the FRET ratio.

Provided are: an enzyme having the activity of converting guanosine in a polynucleotide into a genetic code corresponding to adenosine in an animal cell; a complex of the enzyme; and use of the same. In the present invention, an enzyme having the activity of converting guanosine in a polynucleotide into adenosine is used in an animal cell.

The present invention discloses a GABAT gene of licorice and its application, belonging to the field of genetic engineering technology. The GABAT gene of licorice is any one of the following: (A) having a nucleotide sequence as shown in SEQ ID NO.1; (B) a nucleotide sequence encoding the same protein as the nucleotide sequence shown in (A), but different from the nucleotide sequence shown in (A) due to the degeneracy of the genetic code; (C) a nucleotide sequence having 90%, 95%, or 98% or more sequence homology with the nucleotide sequence shown in (A) and having the same function. The GABAT gene of licorice in the present invention can promote the accumulation of flavonoids in plants, increase the total flavonoid content in plants, and promote the growth and development of plants.

This invention discloses a method for site-specific fluorescent labeling of proteins in cell lysates and its applications, belonging to the field of pharmaceutical bioengineering technology. Utilizing genetic code amplification technology, non-natural amino acids containing azide groups are introduced at specific sites of the target protein. A long-lifetime terbium complex modified with dibenzocyclooctylene is directly added to the cell lysate, enabling copper-free click chemistry labeling of the target protein. This method exhibits good biocompatibility and wide applicability, and can construct CoraFluor-DBCO-labeled protein target libraries. Furthermore, a time-resolved fluorescence resonance energy transfer (TR-FRET) screening system is constructed, utilizing the long fluorescence lifetime of the terbium complex to filter out biological background fluorescence and improve the signal-to-noise ratio. Competitive TR-FRET enables homogeneous screening of small molecule ligands targeting the target protein in the lysate. This method avoids protein purification steps, is simple to operate, and has high screening efficiency.

This invention relates to the field of data processing technology and discloses a digital operation and maintenance management method and system for the entire life cycle of ultra-high voltage insulators. The method includes: acquiring the digital genetic code of the insulator upon leaving the factory and anchoring it on the blockchain; simultaneously collecting dynamic phenotypic characteristics and micro-meteorological parameters related to grid connection; splicing and fusing the underlying heterogeneous data tensors, calculating edge weights based on wind direction and distance attenuation rules, and constructing a dynamic spatiotemporal map base; using a time-series graph convolutional network to perform deep evolutionary inference on the map, outputting a quantitative degradation risk score; dynamically generating differentiated operation and maintenance strategies and underlying control instructions based on this, and mapping virtual storage capacity demand parameters through trend extrapolation; identifying common defects, and issuing process optimization instructions to the manufacturing source after strict tamper-proof verification using blockchain. This invention completely breaks down cross-domain data silos, realizing a closed-loop leap from passive emergency repairs to predictive operation and maintenance and pre-scheduled supply chain scheduling for insulators.

The present invention relates to novel, improved aminoacyl-tRNA synthetase variants, which are useful for genetic code expansion. The present invention also relates to corresponding coding sequences and eukaryotic cell lines comprising such coding sequences. The present invention also relates to methods of preparing a protein of interest (POI) comprising one or more unnatural amino acid residues incorporated by means of the novel aminoacyl-tRNA synthetase variants. The present invention also relates to a method of preparing polypeptide conjugates, wherein a POI generated by means of the present aminoacyl-tRNA synthetase variants is reacted with one or more conjugation partner molecules. Finally, the present invention relates to a kit comprising the required constituents for preparing such POIs comprising one or more unnatural amino acid residues.

The present invention belongs to the field of biotechnology, and specifically relates to a method for site-specific introduction of non-natural amino acid modification in specific amino acid residues of prolyl oligopeptidase (POP) based on genetic codon expansion technology and bio-orthogonal translation system. The method comprises using genetic codon expansion technology to introduce an early stop codon in the coding region of a POP protein gene, using a bio-orthogonal translation system to read through an amber stop codon UAG, and introducing the non-natural amino acid into the POP protein in the presence of the non-natural amino acid, which can not only conditionally control the expression of the POP protein, but also can use the non-natural amino acid containing a special group to specifically label the POP protein, and form a stable covalent bond between proteins interacting with the cell through light cross-linking, so as to provide a powerful means for studying the interaction between POP proteins in the cell; and can also be used for studying the function and post-translational modification of the POP protein.

The invention relates to a genetic coding pyruvic acid fluorescent probe capable of locating a subcellular structure, a preparation method of the genetic coding pyruvic acid fluorescent probe and application of the genetic coding pyruvic acid fluorescent probe in macrophages. The probe comprises a pyruvic acid sensitive recognition structural domain, a fluorescence signal report structural domain and a subcellular localization signal peptide which can perform targeted expression in a specific subcellular structure of macrophages, so that real-time monitoring and quantitative analysis of the concentration of pyruvic acid are realized. The probe provided by the invention has the advantages of strong specificity, high sensitivity, rapid response and the like, and is suitable for cell metabolism dynamic research, power-assisted metabolism mechanism analysis and drug target discovery. In the aspect of disease research, abnormal metabolism of macrophages is closely related to myocardial infarction and the like, the malic enzyme 2 is regulated and controlled to improve the pyruvic acid level, improve the polarization state of the macrophages and enhance the anti-inflammatory and tissue repair functions of the macrophages, and the potential protection effect on ischemic heart tissue is shown. The probe disclosed by the invention is expected to be used as an important tool for researching macrophage metabolism regulation and related disease intervention means.

The present invention relates to novel, site-specifically modified immunoglobulin molecules having the ability to bind to human epidermal growth factor receptor 2 (HER2) carrying in predetermined positions non-canonical amino acid residues (ncAAs); respective nucleic acid sequences encoding such modified immunoglobulin molecules; recombinant organisms useful for preparing such modified immunoglobulin molecules and adapted to express respective coding nucleic acid sequences; methods of preparing said site-specifically modified immunoglobulin molecules; conjugates formed between said site-specifically modified immunoglobulin molecules and a conjugation partner carrying a functional group reactive with said ncAA residues of the immunoglobulin molecule, and more particularly antibody-drug conjugates (ADCs). The invention also relates to specific conjugation partners and their preparation. The invention also relates to pharmaceutical compositions comprising such conjugates; as well as the use of such conjugates in medicine, in particular in the treatment of cancers overexpressing HER2.

The invention discloses a method for specific covalent coupling of a monoclonal antibody drug by using a Z structural domain, which comprises the following steps: introducing non-natural amino acid fluorosulfonic acid-L-tyrosine (FSY) capable of being covalently cross-linked at a specific site of the Z structural domain by a genetic code expansion technology, and converting non-covalent interaction between the Z structural domain and an IgG monoclonal antibody drug into covalent interaction; the binding force of the two molecules is enhanced, the stability is good under the physiological condition, stable and irreversible binding is realized, and the stability and uniformity of the coupled molecules can be remarkably improved. The method can be used as an XDC (antibody coupling medicine, antibody coupling probe, antibody coupling diagnostic reagent and the like) platform method, and is compatible with various loads such as medicine molecules, imaging molecules, nucleic acid modification and the like.

The invention discloses non-natural amino acid inserted ferritin as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The recombinant ferritin 5F-HFn is efficiently prepared by inserting an unnatural amino acid 4-azido-phenylalanine containing an azido group into the 5th site in a natural amino acid sequence of the human heavy-chain ferritin HFn through a genetic code expansion technology based on termination codon inhibition and combining a double-plasmid transformation and nickel column purification process. A copper-free click reaction is utilized to realize directional coupling with a dibenzocyclooctyne modified DNA fragment or other functional molecules, so that the nano-carrier is endowed with multifunctional characteristics of targeted delivery, biological imaging and the like, and a nano-carrier platform capable of being flexibly configured is constructed.

A fully genetically encoded NAD+protein probe based on resonance energy transfer, and a preparation method therefor and the use thereof. Specifically provided is a fully genetically encoded NAD+protein probe, which is formed by means of linking a resonance energy transfer donor, an NAD+responsive protein and a resonance energy transfer receptor in series, wherein the NAD+ responsive protein is a mutant of a DNA ligase; the sequence of the mutant of the DNA ligase is as shown in SEQ ID NO.3 or SEQ ID NO.6; the resonance energy transfer donor is selected from luciferase or a fluorescent protein; the resonance energy transfer receptor is selected from a fluorescent protein; and the fluorescent protein as the resonance energy transfer receptor is different from that of the resonance energy transfer donor. The protein probe can be synthesized in living cells and used for detecting the concentration of NAD+ in living cells.