11 results about "Genetic code" patented technology

This invention discloses a method for preparing and applying plant sulfonated small peptide PSK based on synthetic biology, belonging to the field of biotechnology. Based on the design concept of synthetic biology, this invention constructs an orthogonal synthetic system composed of genetic code expansion technology and SUMO fusion protease digestion technology. A specific PSK dotted vector and a pUltra-sY vector are co-transformed into *E. coli* to construct a genetically engineered strain. The sulfonated small peptide PSK precursor is expressed in this genetically engineered strain, and mature plant sulfonated small peptide PSK is obtained through specific hydrolysis by SUMO protease. This method significantly improves the synthesis efficiency and purity of sulfonated small peptides and has broad application prospects in agricultural production and biotechnology.

The Quadruplet Expanded DNA (QED) eukaryote genetic code comprising twenty nondegenerate QED codons encode proteins (the protein-encoding codons), and thirty-five nondegenerate QED codons (the noncoding codons) being highly correlated with cis-regulatory elements control and regulate transcription, alternate splicing, and polymerization in eukaryotic protein synthesis using canonical amino acids. The QED eukaryote genetic code is an advancement to gene therapeutics that allows for the correction of dysfunctional proteins. Additionally, the QED eukaryote genetic code is further applicable for changing paradigms relating to identifying cures for monogenic rare, multigene cancer, and neurodegenerative diseases.

The invention relates to a computer-implemented method performed by one or more data processing apparatus for assessing a biological feature of a biological macromolecule, preferably a protein molecule, by providing an electrostatic data based in silico representation of the biological macromolecule. The biological feature may be, without limitation, multiple function-related states of the macromolecule, differences in the conformation, e.g. in the secondary structure, patterns based on primary structure, folded nature of the protein including, among others, π-π relationships and relationship between the genetic code and the electrostatic state of the environment of the encoded amino acid(s). The invention can be used among others in the field of drug research, medical diagnosis, genetic engineering and macromolecular modeling.

Provided are cells that are resistant to mobile genetic elements or horizontal gene transfer, and methods for obtaining said cells. Also provided are methods for preventing the horizontal transfer of genetic information between a mobile genetic element and a first cell, cells making use of new genetic codons schemes and related subject matter, kits comprising mutually orthogonal cells, and mobile genetic elements. Also provided are methods of altering the susceptibility of a gene to mutations that alter the encoded amino acid sequence, methods for evolving or improving a protein, and methods for rendering a target gene more resistant to mutation. Additionally provided are uses of the cells for making polymers and methods comprising using the cells for making polymers.

The present application relates to the construction and application of the EcORep-based continuous directed evolution reporter system of aminoacyl-tRNA synthetase, and specifically, the reporter system comprises: a green fluorescent protein mutant sfGFP containing amber stop codon TAG N150TAG ; a chloramphenicol acetyltransferase mutant CAT containing amber stop codon TAG D112TAG ; a linear replicon containing aminoacyl-tRNA synthetase; a reporter plasmid containing a plus-strand mismatch DNA polymerase to continuously mutate the linear replicon, thereby generating an in-vivo library of aminoacyl-tRNA synthetase mutants; and a tRNA, which is orthogonal to the aminoacyl-tRNA synthetase and enables the incorporation of unnatural amino acids and the complete expression of proteins. The present application proves that the evolution reporter system can realize the efficient directed evolution of aminoacyl-tRNA synthetase, and further expands the application range of genetic code expansion technology.

The present invention relates to novel bifunctional molecules capable of undergoing bioorthogonal reactions and inducing proximity between two proteins, methods of inducing proximity between two proteins employing said novel molecules and bioorthogonal reactions and genetic code expansion, methods of evaluating protein-protein proximity interactions employing the novel bifunctional molecules, medical uses of the novel molecules, and methods of treatment of a disease involving post-translational modifications of a protein employing the novel bifunctional molecules.

The invention provides an assembled and annotated chloroplast genome of euphorbia splendens, and relates to the fields of molecular biology and plant genetics. The chloroplast genome of the pteris amurensis is a single cyclic molecule; the total length of the chloroplast genome of the psidium aquilinum is 149180bp; the nucleotide sequence of the chloroplast genome of the pteris amurensis is as shown in SEQ ID NO. 1; the GC content of the chloroplast genome of the pteris amurensis is 43.14%; the pteridium aquilinum chloroplast genome provided by the invention enriches a pteridium gene database, lays a foundation for exploring pteridium aquilinum evolution history and an adaptation mechanism of pteridium aquilinum to environmental stress, decrypts genetic codes of pteridium aquilinum from a molecular level, and is applied to biodiversity protection, plant ecological restoration and gardening.

The invention relates to the field of biosensing, in particular to a genetic coding fluorescent probe for detecting lactic acid change in autophagosome and application of the genetic coding fluorescent probe. The invention discloses a genetic coding fluorescent probe for detecting lactic acid change in an autophagy body, which comprises an FRET pair, a lactic acid sensing structural domain and an autophagy body targeting structural domain and is used for specifically detecting the lactic acid concentration change in the autophagy body in real time, when the lactic acid concentration in the autophagy body changes, the conformation of the probe changes, and the change of the lactic acid concentration in the autophagy body changes. As a result, the fluorescence resonance energy transfer efficiency changes, and dynamic, non-invasive and high-temporal-spatial-resolution detection of the concentration of the lactic acid in the autophagy body can be realized by monitoring the change of the FRET ratio.

This invention discloses a method for site-specific fluorescent labeling of proteins in cell lysates and its applications, belonging to the field of pharmaceutical bioengineering technology. Utilizing genetic code amplification technology, non-natural amino acids containing azide groups are introduced at specific sites of the target protein. A long-lifetime terbium complex modified with dibenzocyclooctylene is directly added to the cell lysate, enabling copper-free click chemistry labeling of the target protein. This method exhibits good biocompatibility and wide applicability, and can construct CoraFluor-DBCO-labeled protein target libraries. Furthermore, a time-resolved fluorescence resonance energy transfer (TR-FRET) screening system is constructed, utilizing the long fluorescence lifetime of the terbium complex to filter out biological background fluorescence and improve the signal-to-noise ratio. Competitive TR-FRET enables homogeneous screening of small molecule ligands targeting the target protein in the lysate. This method avoids protein purification steps, is simple to operate, and has high screening efficiency.

This invention relates to the field of data processing technology and discloses a digital operation and maintenance management method and system for the entire life cycle of ultra-high voltage insulators. The method includes: acquiring the digital genetic code of the insulator upon leaving the factory and anchoring it on the blockchain; simultaneously collecting dynamic phenotypic characteristics and micro-meteorological parameters related to grid connection; splicing and fusing the underlying heterogeneous data tensors, calculating edge weights based on wind direction and distance attenuation rules, and constructing a dynamic spatiotemporal map base; using a time-series graph convolutional network to perform deep evolutionary inference on the map, outputting a quantitative degradation risk score; dynamically generating differentiated operation and maintenance strategies and underlying control instructions based on this, and mapping virtual storage capacity demand parameters through trend extrapolation; identifying common defects, and issuing process optimization instructions to the manufacturing source after strict tamper-proof verification using blockchain. This invention completely breaks down cross-domain data silos, realizing a closed-loop leap from passive emergency repairs to predictive operation and maintenance and pre-scheduled supply chain scheduling for insulators.

The invention discloses non-natural amino acid inserted ferritin as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The recombinant ferritin 5F-HFn is efficiently prepared by inserting an unnatural amino acid 4-azido-phenylalanine containing an azido group into the 5th site in a natural amino acid sequence of the human heavy-chain ferritin HFn through a genetic code expansion technology based on termination codon inhibition and combining a double-plasmid transformation and nickel column purification process. A copper-free click reaction is utilized to realize directional coupling with a dibenzocyclooctyne modified DNA fragment or other functional molecules, so that the nano-carrier is endowed with multifunctional characteristics of targeted delivery, biological imaging and the like, and a nano-carrier platform capable of being flexibly configured is constructed.