3results about How to "Short fermentation time" patented technology

This invention provides an engineered bacterium for producing ectoine, its preparation method, and its application. The engineered bacterium is prepared using a method comprising the following steps: using *Corynebacterium glutamicum* as the starting strain, feedback inhibition by aspartate kinase LysC is relieved, the activity of the gene lysE encoding lysine efflux permease is reduced, and the ectoine synthesis gene cluster ectABC is expressed. Based on this, the activity of any one or at least two of the following—diaminobutyrate acetyltransferase EctA, pyruvate carboxylase Pyc, or aspartate kinase LysC—is enhanced, and / or the activity of any one or at least two of the following—maloquinone oxidoreductase Mqo or homoserine dehydrogenase Hom—is reduced to further increase ectoine yield.

This invention discloses a method for preparing rum with high total ester content. The method includes the following steps: pretreating molasses; preparing a first fermentation medium using the pretreated molasses; expanding the culture of fissile yeast seed liquid in the first fermentation medium to obtain fissile yeast fermentation broth; continuously adding the pretreated molasses to the fissile yeast fermentation broth, culturing to obtain a fermentation product, wherein the residual sugar content of the fermentation product is controlled at 0.5-2%; preparing a second fermentation medium using the pretreated molasses; inoculating the fermentation bacteria into the second fermentation medium, culturing to obtain a fermentation broth; the fermentation bacteria include at least one of Clostridium acetic acid and Acetobacter pasteurella; adding the fermentation broth to the fermentation product, culturing to obtain a mash; continuously distilling the mash, using a kettle heating method for three-part distillation, mixing the second fraction with oak shavings, and aging; obtaining the base spirit, which is then blended to obtain rum with high total ester content. This method can produce mash with high alcohol content and high ester content in a short time.

The invention discloses a mutant for synthesizing ergothioneine and application thereof, and relates to the technical field of gene engineering and synthetic biology, the mutant comprises mutant ergothioneine synthetase and mutant cysteine sulfoxide lyase, the amino acid sequence of the mutant ergothioneine synthetase is selected from any one of SEQ ID No: 5, SEQ ID No: 6, SEQ ID No: 7 and SEQ ID No: 8, and the amino acid sequence of the mutant cysteine sulfoxide lyase is selected from any one of SEQ ID No: 9, SEQ ID No: 10 and SEQ ID No: 11. According to the invention, mutant ergothioneine synthetase and mutant cysteine sulfoxide lyase with high activity are obtained; the two mutant types obtained through screening are used for producing ergothioneine, the advantages of being high in acid yield, short in fermentation time and the like are achieved, the material cost is greatly saved, and industrial production is facilitated.