400 results about "MICROBIAL CONCENTRATION" patented technology

The invention relates to the technical field of water quality monitoring, in particular to a water quality monitoring analysis method and system of a water conservancy system. The method comprises the following steps: acquiring area scale and water area type distribution, and carrying out sensing planning layout and water quality index real-time monitoring to obtain a water conservancy system water quality index data set; performing regional water quality index distribution coupling and regional time step division on the water conservancy system to obtain a corresponding water conservancy pH value, water conservancy dissolved oxygen concentration, water conservancy turbidity, water conservancy heavy metal concentration and water conservancy microorganism concentration; performing microorganism growth efficiency evaluation and microorganism pollution attenuation analysis on the water conservancy microorganism concentration to obtain a water conservancy area microorganism pollution degradation attenuation rate; and performing water quality pollution anomaly quantification and anomaly category decision management on the water quality index data set of the water conservancy system to generate a water quality anomaly category management strategy so as to execute corresponding regional water quality category management work of the water conservancy system. The accuracy and efficiency of water quality monitoring can be improved.

The invention provides a broad-spectrum antibacterial peptide, an antibacterial agent and application, and belongs to the technical field of biological medicine. The invention provides a broad-spectrum antibacterial peptide. The broad-spectrum antibacterial peptide comprises an amino acid sequence as shown in SEQ ID No. 1. The minimum inhibitory concentration of the antibacterial peptide to gram-positive bacteria, gram-negative bacteria and fungi proves that the antibacterial peptide has obvious antibacterial activity to the gram-positive bacteria, the gram-negative bacteria and the fungi. The invention also verifies the minimum inhibitory concentration of the antibacterial peptide to clinical drug-resistant bacteria. The cytotoxicity of the SL-12 disclosed by the invention is relatively weak, the SL-12 only shows obvious cytotoxicity at 200 [mu] g / ml, and the minimum inhibitory concentration of the SL-12 is 6.25-12.5 [mu] g / ml; the SL-12 has no obvious hemolytic activity when the concentration is 100 [mu] g / ml or lower, and is high in safety.

The invention relates to the field of gene engineering, and particularly discloses a barnacle chitin antibacterial peptide, an engineering algal strain of the barnacle chitin antibacterial peptide and a preparation method and application of the engineering algal strain. Wherein the amino acid sequence of the barnacle chitin antibacterial peptide is as shown in SEQ ID NO. 1; or an amino acid sequence which is obtained by substitution and / or deletion and / or addition of one or more amino acids of SEQ ID NO.1 and has the same function as SEQ ID NO.1; or an amino acid sequence which has 70%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% homology with the SEQ ID NO.1 and has the same function as the SEQ ID NO.1. The brand-new barnacle chitin antibacterial peptide obtained in the application has a good antibacterial effect, bacteria can be killed through a cell protection barrier, and the cell hemolysis rate at the minimum inhibition concentration is 7.2%.

The invention relates to the technical field of production parameter control, and particularly discloses an organic fertilizer production parameter optimization control method for a compound microbial agent, which comprises the following steps of: calculating a temperature fluctuation index and a pH stability index according to temperature data and a pH value in a fermentation stage so as to quantitatively evaluate the stability of a fermentation environment; evaluating the external interference degree by using the normalized comprehensive influence factor, intelligently analyzing the microbial ecological disorder condition through clustering analysis and a decision tree algorithm in combination with microbial concentration data, and calculating a microbial ecological disorder fluctuation coefficient; based on an evaluation result, dynamically calculating an adjustment factor, accurately adjusting the temperature and the pH value in the fermentation condition, and ensuring that the microbial flora is in an optimal active state; finally, whether the fermentation parameters reach the standard or not is determined by re-evaluating the adjusted flora state, so that the quality and the production efficiency of the organic fertilizer are ensured.

The invention relates to the technical field of antibacterial peptide application, in particular to an antibacterial peptide for inhibiting drug-resistant staphylococcus aureus and application of the antibacterial peptide. The antibacterial peptide is obtained by performing N-terminal acetylation modification and C-terminal amidation modification on polypeptide as shown in SEQ ID NO.1. The invention further discloses a preparation method of the antibacterial peptide. The antibacterial peptide provided by the invention is a novel cationic antibacterial peptide, the minimum inhibitory concentrations of the antibacterial peptide to pathogenic bacteria staphylococcus aureus ATCC12600 and staphylococcus aureus ATCC33591 are respectively 4 [mu] g / mL and 32 [mu] g / mL, the biological safety is extremely high, and due to the characteristics, the antibacterial peptide shows extremely high medical drug antibacterial value, and can be used for preparing the antibacterial peptide. Alternative medicinal resources are provided for clinically resisting the two human pathogenic bacteria in the future.

The invention discloses an antibacterial peptide and application thereof. The amino acid sequence of the antibacterial peptide disclosed by the invention is KKKEHRLLHRVLFHRL. The minimum inhibitory concentration of the antibacterial peptide to staphylococcus aureus is 4.5 mu g / mL, and the minimum bactericidal concentration of the antibacterial peptide to staphylococcus aureus is 72.7 mu g / mL. The antibacterial peptide disclosed by the invention has remarkable antibacterial activity on staphylococcus aureus and is expected to be widely applied as a novel bacteriostatic agent for preventing and treating staphylococcus aureus.

The invention discloses a milk-derived broad-spectrum antibacterial peptide and application thereof, and relates to the technical field of biology. The amino acid sequence of the antibacterial peptide is as shown in any one of SEQ ID NO.2-6. Five novel antibacterial peptides are obtained by carrying out symmetrical structure design and amino acid mutation on the basis of a template peptide, and tests find that the five novel antibacterial peptides have broad-spectrum antibacterial activity which is obviously higher than that of the template peptide, and basically have no cytotoxicity. Wherein the geometric mean (GM) of the minimum inhibitory concentration can reach 3.60, and the therapeutic index (TI) can reach 71.11. The antibacterial peptide prepared by the invention still keeps good antibacterial activity in physiological salt concentration and protease environment, has certain serum stability, and has higher application value; in addition, the antibacterial peptide has a good inhibition effect on the formation of a bacterial biofilm, and has a synergistic effect with traditional antibiotics.

The invention belongs to the technical field of antibacterial peptides, and particularly relates to an antibacterial peptide and application thereof. The amino acid sequence of the antibacterial peptide is shown as SEQ ID NO: 1, the number of amino acids is small, the synthesis cost is low, the antibacterial peptide has high antibacterial activity on gram-negative bacteria and gram-positive bacteria, the minimum inhibitory concentration of the antibacterial peptide on acinetobacter baumannii, escherichia coli, pseudomonas aeruginosa and staphylococcus aureus is 4.68-37.5 mu g / mL, and the antibacterial peptide has the advantages of broad-spectrum antibacterial property, good stability and good application prospect. No obvious cytotoxicity and hemolysis effect exist; moreover, end-to-end amido bond cyclization is carried out, so that the antibacterial activity is further enhanced, the antibacterial effect on acinetobacter baumannii, escherichia coli, pseudomonas aeruginosa and staphylococcus aureus is enhanced by 2-4 times, and the minimum inhibitory concentration is reduced to 2.34-9.38 mu g / mL; after the end-to-end amido bonds are cyclized, the plasma stability and safety of the antibacterial peptide are further improved, and the cytotoxicity and hemolysis are reduced.

The invention provides an antibacterial peptide and application thereof, and belongs to the technical field of biological medicine. Based on bioinformatics and an AI technology, a novel broad-spectrum antibacterial peptide is expected to be screened from tick (hard tick shoulder) genomes, and the antibacterial peptide has antibacterial activity on bacteria (including clinical drug-resistant strains) and fungi, does not have obvious cytotoxicity and hemolytic activity, and is an important candidate molecule of anti-infective drugs. In the embodiment of the invention, the minimum inhibitory concentration of the antibacterial peptide on gram-positive bacteria, gram-negative bacteria and fungi is verified, and the antibacterial peptide is proved to have obvious antibacterial activity on the gram-positive bacteria, the gram-negative bacteria and the fungi. The invention also verifies that the minimum inhibitory concentration of the antibacterial peptide to clinical drug-resistant bacteria is equivalent to the minimum inhibitory concentration of the antibacterial peptide to standard strains, and verifies that the antibacterial peptide can be used as a broad-spectrum antibacterial peptide and is named as SL-15.

The invention relates to the field of animal breeding, and discloses a pig beta defensin compound preparation which comprises pig beta defensin PBD1, PBD2 and PBD114, and the weight ratio of the PBD1 to the PBD2 to the PBD114 is 1: 1: 1. The porcine beta defensin PBD1, the porcine beta defensin PBD2 and the porcine beta defensin PBD114 are recombinant proteins, and are respectively expressed in escherichia coli BL21 (DE3) PLysS competent cells by a pET-32a expression vector. Through the synergistic effect of the defensins PBD1, PBD2 and PBD114, the minimum inhibitory concentration (MIC) of the EPEC is reduced to 18.75 mu g / mL from 75 mu g / mL of single defensins, and is reduced by 75%; within 24 hours, the bacteriostasis rate of the compound preparation reaches 90%, and the inhibition effect on EPEC is remarkably improved.

The invention belongs to the field of biological medicine, and particularly relates to an antibacterial peptide for inhibiting staphylococcus aureus and pseudomonas aeruginosa and application of the antibacterial peptide. The antibacterial peptide CAMP648NC is a novel cationic antibacterial peptide, the minimum inhibitory concentrations of the antibacterial peptide CAMP648NC to pathogenic bacteria staphylococcus aureus and pseudomonas aeruginosa are 16 [mu] g / mL and 32 [mu] g / mL respectively, the antibacterial peptide CAMP648NC has extremely low cytotoxicity, and after human skin keratinocytes HaCat and bronchial epithelial cells BEAS-2B are treated by the antibacterial peptide CAMP648NC with the concentration of 128 [mu] g / mL for 24 hours, the survival rates of 110.4% and 87.3% are still kept. Due to the characteristics, the compound shows extremely high medical drug antibacterial value, and alternative medicinal resources are provided for clinically resisting the two human pathogenic bacteria in the future.

The present invention provides a sulfur autotrophic denitrification filler and a preparation method thereof. The sulfur autotrophic denitrification filler of the present invention comprises the following raw materials in parts by weight: 50-100 parts of sulfur powder, 5-20 parts of pH buffer, 10-50 parts of medical stone, and 0-10 parts of additives; wherein the pH buffer is one or more of sodium carbonate, sodium bicarbonate, sodium hydroxide, and potassium hydroxide; and the additive is one or more of amide substances and epoxy resins. The sulfur autotrophic denitrification filler has good filler strength and a long service life, and can be conducive to the attachment of denitrifying thiobacillus to the surface of the sulfur autotrophic denitrification filler, thereby increasing the microbial concentration per unit volume of the filler and increasing the reaction rate.

The invention provides a recombinant chicken antibacterial peptide aiming at drug-resistant staphylococcus aureus, and belongs to the technical field of veterinarians. The recombinant chicken antibacterial peptide is expressed through genetic engineering recombinant escherichia coli. Three natural chicken antibacterial peptide active regions are fused, and two disulfide bonds are introduced to stabilize the structure of the recombinant chicken antibacterial peptide and enhance the affinity of the recombinant chicken antibacterial peptide to staphylococcus aureus outer membrane protein. The minimum inhibitory concentration of the recombinant chicken antibacterial peptide to the drug-resistant staphylococcus aureus is obviously lower than that of three natural chicken antibacterial peptides, and the formation of a biomembrane of a USA300 strain of the drug-resistant staphylococcus aureus can be thoroughly inhibited when the minimum inhibitory concentration is 8 mu g / mL. The recombinant chicken antibacterial peptide has a good antibacterial effect on different drug-resistant staphylococcus aureus separated from a chicken house, and can effectively solve the infection problem of the drug-resistant staphylococcus aureus in the chicken house.

The invention relates to the technical field of monitoring analysis, and discloses a microplastic degradation process monitoring system based on microbiological treatment, which comprises the following modules: a microbiological culture module for culturing microorganisms in a microplastic degradation process and adjusting a plurality of parameters through an environmental reactor to maintain the activity of the microorganisms; the data monitoring and processing module is used for monitoring and acquiring a plurality of key indexes of the micro-plastics in the degradation liquid in the degradation process through a plurality of sensors, removing and supplementing abnormal data, and finally synchronously uploading non-abnormal data to the cloud through the Internet of Things node; and the intelligent analysis platform comprises a degradation prediction unit and a reporting unit. Through one-way comparison and relevance comparison, abnormal data monitored by the sensor are removed, the microplastic degradation efficiency is predicted by using the living environment of microorganisms, the concentration of the microorganisms and a degradation kinetic model, and the microplastic degradation efficiency can be comprehensively analyzed in combination with multiple parameters during microplastic degradation analysis.

The invention provides a modified antibacterial peptide, and belongs to the technical field of antibacterial peptides. The invention provides a modified antibacterial peptide. The modified antibacterial peptide comprises at least one of a first antibacterial peptide, a second antibacterial peptide, a third antibacterial peptide, a fourth antibacterial peptide, a fifth antibacterial peptide and a sixth antibacterial peptide, the amino acid sequences of the first antibacterial peptide, the second antibacterial peptide, the third antibacterial peptide, the fourth antibacterial peptide, the fifth antibacterial peptide and the sixth antibacterial peptide are shown as SEQ ID NO.1-SEQ ID NO.6 in sequence. On the basis of prevotelin-2, the modified antibacterial peptide is obtained by reducing negative charge amino acid, increasing positive charge amino acid, introducing hydrophobic amino acid, adjusting or deleting an amino acid sequence and optimizing a polypeptide structure. In-vitro minimum inhibitory concentration determination results show that compared with an initial peptide, the modified antibacterial peptide has significantly enhanced antibacterial activity on bacteria and fungi, and also has an obvious bactericidal effect on clinical drug-resistant bacteria.

The invention relates to a preparation method and application of recombinant canine lactoferrin and peptide thereof, aiming at codon preference of host cells, canine lactoferrin genes are optimized, the full length of canine lactoferrin and the lactoferrin peptide are successfully expressed by virtue of an escherichia coli expression system, and the recombinant canine lactoferrin and the peptide thereof are identified by virtue of a minimum inhibitory concentration experiment. It is proved that the canine lactoferrin and the peptide thereof have the inhibiting capacity on pathogenic bacteria such as staphylococcus aureus, salmonella typhimurium and staphylococcus pseudointermedius, and the antibacterial activity MIC of the canine lactoferrin on the staphylococcus aureus, the salmonella typhimurium and the staphylococcus pseudointermedius is 1.5 mg / mL; the antibacterial activity MIC of the dog lactoferrin peptide to staphylococcus aureus is equal to 0.75 mg / mL, and the dog lactoferrin peptide shows high antibacterial ability.

The utility model provides a novel sulfur autotrophic denitrification nitrogen removal reactor, which comprises a clear water zone, a mud-water separation zone, a buffer zone, a reaction zone, a water distribution zone and an internal reflux pump, the clear water zone is arranged at the top of the reactor, the mud-water separation zone is arranged below the clear water zone in the reactor, the buffer zone is arranged at the bottom of the mud-water separation zone, and the reaction zone is arranged in the internal reflux pump. A reaction zone is arranged below the buffer zone in the reactor, and a water distribution zone is arranged below the reaction zone in the reactor. Inclined tube filler is arranged in a mud-water separation area in the reactor, suspended filler is arranged in a reaction area in the reactor, and a buffer area and a water distribution area form a closed loop through an internal reflux pump. According to the fluidized bed type sulfur autotrophic reactor, the suspended filler is added into the fluidized bed type sulfur autotrophic reactor and is fixed in the reaction area, and sulfur autotrophic bacteria can be attached to the filler, so that the concentration of microorganisms in the reactor is improved, mud is not easy to run, and meanwhile, the denitrification efficiency is improved.

The invention relates to an enzyme response antibacterial carbon dot and a preparation method and application thereof.The carbon dot takes a copper-doped carbon dot as a core and is covalently grafted with a hyaluronic acid shell layer through amidation reaction to form a composite nano material Cu-CDs (at) HA, specifically, soluble copper salt and folic acid are subjected to a hydrothermal reaction to obtain the copper-doped carbon dot, carboxyl is activated through EDC / NHS in a buffer system, and the carbon dot is prepared. And performing grafting reaction with hyaluronic acid to obtain a target product. The hyaluronidase-containing antibacterial composition can be specifically degraded and release antibacterial components under the action of hyaluronidase, shows good bacterial responsive bactericidal performance, has the minimum inhibitory concentrations of 8 g / mL and 16 g / mL for staphylococcus aureus and escherichia coli respectively, and has peroxidase-like activity and free radical scavenging capacity. The antibacterial carbon dots can be used for preparing intelligent wound dressings, antibacterial coatings and other biomedical materials, and have the advantages of responsive drug release, efficient antibacterial property and promotion of wound repair.

The invention discloses an application of 12-ketolithocholic acid in preparation of an anti-Vihonor coccus product. The first test proves that the 12-ketolithocholic acid has good activity of inhibiting atypical Wehonor coccus, specifically, the minimum inhibitory concentration required for inhibiting 50% of tested ATCC 17744 strain is measured, and the result shows that bacteria in a blank control hole containing no medicine grow obviously, and the MIC50 of the 12-ketolithocholic acid for the atypical Wehonor coccus is 294.7 mu g / ml. Therefore, the new application of the 12-ketolithocholic acid provided by the invention provides reference for clinical prevention and control of epidemic diseases caused by Wehonor coccus.

The invention provides application of chlorogenic acid in inhibition of growth of carbapenem-resistant Escherichia coli, and belongs to the technical field of development of antibacterial materials. The invention discloses an application of chlorogenic acid in inhibiting the growth of carbapenem-resistant Escherichia coli, the chlorogenic acid can inhibit the growth of the carbapenem-resistant Escherichia coli according to the good in-vitro killing effect on the carbapenem-resistant Escherichia coli, the minimum inhibitory concentration is 5mg / mL, and the chlorogenic acid can inhibit the growth of the carbapenem-resistant Escherichia coli. The invention provides the inhibition effect of the chlorogenic acid on the carbapenem-resistant Escherichia coli, and the chlorogenic acid has a wide application value in the fields of medicines and the like, and can effectively destroy the biological membrane of the carbapenem-resistant Escherichia coli, so that the invention provides the inhibition effect of the chlorogenic acid on the carbapenem-resistant Escherichia coli.

The invention provides a cooling tower cleaning period decision-making method based on filler layer resistance characteristic online identification, which comprises the following steps: acquiring multi-dimensional time sequence data such as differential pressure, water temperature, conductivity, turbidity and microbial concentration based on a multi-source sensor, and constructing a structured trend characteristic matrix through time synchronization, abnormal value elimination and statistical characteristic extraction; in combination with expert knowledge, constructing a multi-modal combined pollution knowledge graph, and learning pollution factor interaction weights by using a graph neural network to realize causal reasoning of combined pollution mechanisms such as scaling, biological slime and the like; according to the method, an abnormal causal chain path is efficiently matched in a knowledge graph by adopting improved A * search, causes and evolution stages are output, automatic attribution and intelligent cleaning suggestion optimization are realized, and the resistance abnormity diagnosis accuracy and the adaptability of a cleaning strategy are improved by continuously feeding back diagnosis results and dynamically expanding a knowledge graph structure.

The invention relates to the technical field of application of antibacterial peptides, in particular to a broad-spectrum bacterial-source antibacterial peptide cAMP-919nc and application of the broad-spectrum bacterial-source antibacterial peptide cAMP-919nc. The antibacterial peptide cAMP-919nc is obtained by carrying out N-terminal acetylation modification and C-terminal amidation modification on a polypeptide as shown in SEQ ID NO.1. The invention further discloses a preparation method of the antibacterial peptide cAMP-919nc. The antibacterial peptide shows an excellent antibacterial effect in various bacteria, and particularly, the minimum inhibitory concentration of the antibacterial peptide to staphylococcus aureus is as low as 4 mu g / mL. Under different pH conditions, the antibacterial peptide also shows remarkable bactericidal activity on gram-negative pathogenic bacteria acinetobacter baumannii. In addition, the peptide has extremely low cytotoxicity and shows good safety. The invention provides the antibacterial peptide with excellent performance, and the antibacterial peptide has wide application potential and can be used for developing various antibacterial products such as antibacterial drugs, disinfectants and biological preservatives. The invention opens up a new direction for the research and application of the antibacterial peptide, and has important scientific and practical values.

The invention provides an antibacterial peptide CN-3 and application thereof, and relates to the technical field of peptide inhibitors. The amino acid sequence of the antibacterial peptide CN-3 is GPGGAWAAKVIS, and is as shown in SEQ ID NO. 1. The minimum inhibitory concentration (MIC) of the antibacterial peptide CN-3 to staphylococcus aureus and escherichia coli is 5.0 mg / mL. According to the antibacterial peptide CN-3 and the application thereof, the CN-3 has staphylococcus aureus and escherichia coli bacteriostatic activity, and plays a role by destroying bacterial cell membranes; cN-3 is not easy to generate drug resistance, low in cytotoxicity and high in cell selectivity, and can be used for preparing an antibacterial composition for treating staphylococcus aureus and escherichia coli infection.

The invention relates to the technical field of antibacterial peptides, in particular to application of an antibacterial peptide RV-18-ack in preparation of an antifungal product. The antibacterial peptide RV-18-acy disclosed by the invention comprises a polypeptide RV-18 of which the amino acid sequence is shown as SEQ ID NO. 1. The antibacterial peptide RV-18-ack is a polypeptide capable of forming an amphiphilic alpha helical structure, can form nanoparticles in a self-assembly manner, has broad-spectrum antifungal activity, can damage fungal cell membranes in a targeted manner to play a bactericidal role, shows a relatively good antibacterial role on candida albicans, has the minimum inhibitory concentration of 2.344-75 mu M, and is free of cytotoxicity and hemolysis; besides, the antibacterial peptide RV-18-ack contains 18 amino acids, and all the amino acids are L-type amino acids, so that the production cost is greatly reduced, and the antibacterial peptide RV-18-ack is expected to become a novel broad-spectrum antifungal candidate drug and has a good application prospect in the aspect of resisting super fungi.

The invention relates to the technical field of nano-enzyme preparation and application thereof, in particular to nano-enzyme and a preparation method and application thereof in antibiosis, the nano-enzyme is prepared by using ascorbic acid, sodium hydroxide, copper chloride and cobalt chloride as reaction raw materials and adopting a liquid-phase chemical reduction coprecipitation method, and copper-cobalt nano-enzyme is obtained. The diameter of the prepared nano-material is about 3 nm, the nano-material has good peroxidase-like activity, when a substrate hydrogen peroxide is catalyzed, the Michaelis constant KM is 0.93 mM, the maximum reaction rate Vmax is 12.1862 * 10 <-8 > (M.s <-1 >), the mimic enzyme shows good antibacterial performance on escherichia coli and staphylococcus aureus, the bactericidal effect can be achieved by breaking cell walls, and the mimic enzyme has good application prospects. And the minimum inhibitory concentrations of the compounds are respectively 22.5 [mu] g / mL and 5.63 [mu] g / mL. The nano enzyme is simple in preparation method, low in cost and remarkable in antibacterial effect, and has wide application prospects in the fields of medical industry, chemical production and the like.

The invention discloses a vehicle-mounted microbial environment management system based on multi-mode sterilization control and a method thereof, and relates to the technical field of vehicle-mounted air purification and microbial control. The system comprises a microorganism sensing module used for collecting and detecting the concentration of microorganisms in air in a vehicle in real time, a state monitoring module used for feeding back the irradiation state of an ultraviolet light source and the activity state of a photocatalytic material, and a control module used for judging the pollution risk level based on multi-source information fusion and generating a control instruction. The safety and interaction module is used for passenger state detection and remote control interaction, and the ultraviolet disinfection module, the photocatalytic purification module and the ozone release module cooperatively execute disinfection operation according to a control instruction. Through a closed loop mechanism of pollution detection, risk determination, safety verification, linkage control and effect verification, high-precision, intelligent, safe and reliable vehicle-mounted microbial environment management is realized, the operation efficiency and the use safety of the system are improved, and the system is suitable for various intelligent cabins and high-standard vehicle-mounted environment control application scenes.

The invention relates to a composite microsphere for enhancing the antibacterial performance of baicalin as well as a preparation method and application of the composite microsphere, and relates to the technical field of biological materials. The composite microsphere comprises zein and baicalin, wherein the baicalin is embedded into the hydrophobic interior of the zein. The composite microspheres can reduce the minimum inhibitory concentration of the baicalin and the cell membrane conductivity of the baicalin, and enhance the biological membrane clearance rate of the baicalin and the DNA inhibition effect of the baicalin, so that the antibacterial property of the baicalin is improved, and the composite microspheres have wide application prospects in the industries of biological materials and the like.

The present invention provides use of a C-JUN N-terminal kinase inhibitor, SU3327, in the preparation of a medicament for enhancing the efficacy of polymyxin against bacterial infection, and use of a composition of SU3327 and polymyxin in the preparation of a medicament for an enhanced efficacy against bacterial infection, wherein the polymyxin is preferably Polymyxin E. The invention not only shows an enhanced antibacterial activity of polymyxin by SU3327 in synergy through minimum inhibitory concentration tests in checkerboard assays and in vitro bacterial growth curves, but also confirms the ability of SU3327 to enhance polymyxin along with its effectiveness in vivo in the experiments with a model of mice infected with drug resistant bacteria at the animal level. The invention provides a new use of SU3327 in enhancing antibacterial activities of polymyxin antibiotics, resolving the technical problems such as clinical resistance to polymyxin, low clinical therapeutic index of polymyxin, etc.

The invention relates to the technical field of fan control, and discloses a fan rotating speed adjusting method and system based on a dynamic pollution load. The method comprises the following steps: collecting a target PM2.5 concentration, a target VOC concentration and a target microorganism concentration of each monitoring point, and calculating a comprehensive pollution load index of each monitoring point; traversing boundary critical points in all the monitoring points based on the comprehensive pollution load index and generating a boundary identification vector; the real-time pressure drop of the air inlet side and the air outlet side of the filter element in each fan is collected, and the pressure difference compensation gain is calculated; calculating a target reference rotating speed for all monitoring points in the responsible sector of each fan; and calculating the average pollution load index of the responsible sector of each fan, executing fuzzy reasoning to obtain a rotating speed increment, and generating a PWM duty ratio according to the target reference rotating speed and the rotating speed increment to drive each fan. The technical problems of space pollution response lag and energy consumption imbalance are solved, and the performance of the air purification system is improved.

The invention discloses application of chlorogenic acid in preparation of a product for resisting carbapenem-resistant klebsiella pneumoniae, and belongs to the technical field of medicines. It is verified that chlorogenic acid has a good in-vitro killing effect on carbapenem-resistant klebsiella pneumonia, growth of the carbapenem-resistant klebsiella pneumonia can be inhibited, the minimum inhibitory concentration is 20 mg / mL, and a biological membrane of the carbapenem-resistant klebsiella pneumonia can be effectively destroyed. The invention provides the inhibition effect of chlorogenic acid on carbapenem-resistant klebsiella pneumoniae, and the chlorogenic acid has wide application value in the fields of medicines and the like.