Preparation method of floating embedding bacterium agents for in-situ remediation of oil polluted water

Abstract

The invention relates to a preparation method of floating embedding bacterium agents for in-situ remediation of oil polluted water, specifically relating to a technology for fixing oil degradation bacteria by using expanded graphite, sodium alginate and calcium chloride, absorbing and degrading to purify the petroleum polluted water. The preparation method of the floating embedding bacterium agents comprises the following steps: adding petroleum degrading bacteria liquid into sodium alginate sol containing the expanded graphite, mixing uniformly, dropping into calcium chloride solution and cross-linking overnight at 4 degrees centigrade to obtain the floating embedding bacterium agents fixing hydrocarbon degradation bacteria. The floating embedding bacterium agents prepared by the preparation method has the advantages of high mechanical strength, good floatability and mass transfer performance and high degradation efficiency; the floating embedding bacterium agents can be directly put onto the surface of the petroleum polluted water; the embedding bacterium agents can float on an oil-water interface for long term so as to ensure effective contact between the bacterium agent and the petroleum; the embedding technology increases the concentration of microorganisms, prevents the bacteria influencing by the water using environment, effectively improves the degradation efficiency, recovers and recycles conveniently and has no secondary pollution risk.

Description

technical field [0001] The invention belongs to the technical field of water treatment, and in particular relates to a preparation method of a floating-type embedded bacterial agent used for in-situ restoration of oil-polluted water bodies. Background technique [0002] With the development of the oil exploration industry and the prosperity of the shipping industry, more and more petroleum hydrocarbons enter the water body every year, and the frequent occurrence of sudden oil spill accidents makes oil pollution a hot spot of global concern. Petroleum pollutants can spread rapidly on the surface of the water body and form a layer of oil film to hinder the oxygen mass transfer between the water body and the air, causing the dissolved oxygen in the water body to drop, producing stench, causing the deterioration of water quality and the death of aquatic organisms. Polycyclic aromatic hydrocarbons in petroleum hydrocarbons have strong teratogenic and carcinogenic effects, and can...

AI Technical Summary

Problems solved by technology

[0004] Although microbial remediation technology has achieved a series of achievements, its application still faces some problems: first, oil-degrading microorganisms are isolated, but their use is affected by factors such as the living environment and the competition between indigenous strains, and it is difficult to It is difficult to survive and apply under new environmental conditions; secondly, from the perspective of microbial uptake of petroleum hydrocarbons, microbial degradation of high-carbon hydrocarbons (C ≥ 12) is carried out through direct cell contact, but due to the specific gravity of the petroleum-degrading bacteria itself, its After being put into the water, it is usually in a state of sedimentation, which hinders its effective contact with high-carbon hydrocarbon slick oil

Practical applications have shown that due to the poor strength of expanded graphite materials, it is easy to cause the collapse of the pore structure, thereby affecting the practical application performance of bio-expanded graphite