110 results about "Hydrocarbon degradation" patented technology

The invention relates to a preparation method of floating embedding bacterium agents for in-situ remediation of oil polluted water, specifically relating to a technology for fixing oil degradation bacteria by using expanded graphite, sodium alginate and calcium chloride, absorbing and degrading to purify the petroleum polluted water. The preparation method of the floating embedding bacterium agents comprises the following steps: adding petroleum degrading bacteria liquid into sodium alginate sol containing the expanded graphite, mixing uniformly, dropping into calcium chloride solution and cross-linking overnight at 4 degrees centigrade to obtain the floating embedding bacterium agents fixing hydrocarbon degradation bacteria. The floating embedding bacterium agents prepared by the preparation method has the advantages of high mechanical strength, good floatability and mass transfer performance and high degradation efficiency; the floating embedding bacterium agents can be directly put onto the surface of the petroleum polluted water; the embedding bacterium agents can float on an oil-water interface for long term so as to ensure effective contact between the bacterium agent and the petroleum; the embedding technology increases the concentration of microorganisms, prevents the bacteria influencing by the water using environment, effectively improves the degradation efficiency, recovers and recycles conveniently and has no secondary pollution risk.

The invention provides a method capable of rapidly selecting and screening surfactant petroleum hydrocarbon degradation pure bacterial strain from oil soil. According to the method capable of rapidly selecting and screening the surfactant hydrocarbon degradation bacteria on the surface of forming material, the oil soil is collected from an oil field and added into a hydrocarbon degradation culture medium with the amount of 5%, wherein crude oil of the hydrocarbon degradation culture medium is a sole carbon source, the content of the crude oil is gradually improved, and an enrichment culture is carried out on a constant temperature shaker. After the enriched bacteria liquid is diluted to a certain degree and coated to be cultured on a blood plate, 1-3d is cultured at the temperature of 30 DEG C, bacteria colony with a clear and large transparent zone edge on the blood plate is taken, after the bacteria colony is cultured in a liquid meat peptone culture medium, the bacteria liquid is dipped through a sterilizing toothpick blunt end and dibbled on an oil plate, 3d is cultured at the temperature of 30 DEG C, bacteria colony with an obvious and large oil biting spot is taken to be vaccinated in a meat peptone inclined surface and stored, after the bacteria colony is cultured with the meat peptone culture medium, the hydrocarbon degradation culture medium and the surfactant culture medium are vaccinated in sequence, 7d and 3d are cultured at the temperature of 30 DEG C, the hydrocarbon degradation capacity and the surfactant capacity are determined, and therefore the surfactant petroleum hydrocarbon degradation bacterial strain can be obtained.

The invention discloses a method for repairing high-concentration oil-polluted soil through mycorrhizal fungi-plant-degradation bacterium, concretely comprising the the steps of: inoculating an arbuscular mycorrhizal fungi inoculant such as Glomus mosseae bacteria or Glomus versiforme bacteria and a mixed inoculant of petroleum hydrocarbon and degradation bacterium liquid in the high-concentration oil-polluted soil and planting mycorrhizal fungi aulophytes such as shamrocks and corns, growing for 90-125 days, and harvesting corn plant bodies with high biomass liveweight and burning in a centralized manner. The method has the advantage of establishing a composite mycorrhizal fungi-plant-degradation bacterium co-metabolism system; on one side, the mycorrhizal fungi and plants form mutually beneficial symbionts so as to help the plants to absorb nutrition and water and promote the growth of the plants so that the stress resistance of the plants to the high-concentration oil-polluted soil; on the other side, the mycorrhizal fungi is used as heterotrophic microorganism and the petroleum hydrocarbon is used as exogenous carbon which is decomposed and utilized; and moreover, nutrients are provided to exogenous petroleum hydrocarbon degradation bacteria through mycorrhizal fungi and the secretions of plant root cells so that the degradation efficiency of the petroleum hydrocarbon is improved.

The invention discloses a highly effective degradation strain JN4 for petroleum hydrocarbons in oily sludge and application thereof, which belong to the technical field of bioremediation. The degradation strain JN4 is preserved in the China General Microbiological Culture Collection Center, the preservation number is CGMCC NO.14975, and the classification number is Pseudomonas stutzeri. The strainJN4 with a petroleum hydrocarbon degradation function which is disclosed by the invention is applied to treatment of oily sludge by degrading petroleum hydrocarbons. The degradation rate of the bacteria on total petroleum hydrocarbon (TPH) in oily sludge is 71.05 percent within 30 days. The single strain provided by the invention has obvious degradation capability on n-C10 to n-C30, and GC-MS (gas chromatography-mass spectrometry) analysis indicates that the strain has strong degradation capability on cycloalkane naphthane, indane and d-homoandrostane. The optimum degradation conditions of the bacteria on petroleum pollutants are 25 DEGC to 40 DEG C and the pH value of 5 to 11. The biological method is used in oily sludge remediation technology, and has the advantages of high effectiveness, low cost, environment-friendliness and the like.

Method of modeling the biodegradation of hydrocarbons trapped in an oil reservoir or trap through the action of the bacterial population in an underlying aquifer. From data relative to the reservoir studied, concerning the form and the height of the reservoir, the physical characteristics of the porous medium, the thickness of the transition zone between the hydrocarbons and the water, the composition of the hydrocarbons, of the flow of electron acceptors entering the reservoir and data relative to the bacterial population in the aquifer, modeling is performed by discretizing the reservoir by means of a grid wherein the height of each grid cell is the thickness of the transition zone, and the variation, over the height of the reservoir, of the proportion in heavy fractions of hydrocarbons under the effect of the biodegradation is determined by iterative adjustment in each grid cell of the bacterial population to the amount of hydrocarbons available, the pore space available, the amount of electron acceptors present in the reservoir and the degradation capacities of said population. Applications: determination of the composition of oils in a reservoir and notably of the location of the heaviest fractions.

The invention discloses a bioremediation method for petroleum contamination and its special strains. The strains are (Alcaligenes xylosoxydans)H4-1 with the preservation number of CGMCC No.3235. The strains of the present invention has strong petroleum hydrocarbon degradation capability. The strains, inocula and its application method provide theoretical basis for the bioremediation work of the petroleum contamination soil in our country, have great significance for the environment protection work in the national oil field region and bioremediation work for the contamination due to leakage inthe petroleum transport process speed up the progress of the bioremediation work for petroleum contamination and have important economical significance.

Method of modeling the biodegradation of hydrocarbons trapped in an oil reservoir or trap through the action of the bacterial population in an underlying aquifer.From data relative to the reservoir studied, concerning the form and the height of the reservoir, the physical characteristics of the porous medium, the thickness of the transition zone between the hydrocarbons and the water, the composition of the hydrocarbons, of the flow of electron acceptors entering the reservoir and data relative to the bacterial population in the aquifer, modeling is performed by discretizing the reservoir by means of a grid wherein the height of each grid cell is the thickness of the transition zone, and the variation, over the height of the reservoir, of the proportion in heavy fractions of hydrocarbons under the effect of the biodegradation is determined by iterative adjustment in each grid cell of the bacterial population to the amount of hydrocarbons available, the pore space available, the amount of electron acceptors present in the reservoir and the degradation capacities of said population.Applications: determination of the composition of oils in a reservoir and notably of the location of the heaviest fractions.