110 results about "Hydrocarbon degradation" patented technology

The invention relates to an immobilized microbial inoculant for restoring petroleum-hydrocarbon-polluted alkaline-saline soil and a preparation method thereof. A salt / alkali-tolerant petroleum hydrocarbon degradation bacterium variant corynebacterium B05: (Corynebacterium variabile) HRJ4 (the collection number is CGMCC No.10134) is screened from alkaline-saline petroleum-polluted soil, and sodium alginate is added into a carrier biochar to prepare immobilized bacterium pellets which are stored and used for treating petroleum-hydrocarbon-polluted soil. The immobilized bacterium pellets are prepared by mixing the bacterium suspension, biochar and sodium alginate in a v / w / w ratio of 100:5:2. The invention provides a preparation method of the immobilized bacterium pellets. The immobilized microbial inoculant has the advantages of simple technique, obvious advantages of the degradation bacterium, and high stability, is beneficial to the petroleum hydrocarbon degradation bacterium to adapt to the new polluted environment, enhances and improves the microecological environment quality of the soil, does not cause secondary pollution to the environment, and can obviously enhance the degradation efficiency of petroleum hydrocarbons in the alkaline-saline soil.

The invention discloses an in situ biological repairing method of biomass strengthening petroleum -salt-mixed contaminated soil, which is to add biomass degradation agent into biomass, and then the biomass degradation agent is plowed and landfilled under plough horizon, water logging and salt washing are utilized to reduce soil salt content, and then petroleum hydrocarbon degradation bacteria agent is added to degrade petroleum hydrocarbon, The invention utilizes the biomass to interdict capillary salt uprise phenomenon possibly generated; carbohydrate substance generated by biomass degradation can be taken as a good quality carbon source of the petroleum hydrocarbon degradation bacteria agent, so as to accelerate the growth of the petroleum hydrocarbon degradation bacteria agent; lignin generated by the biological degradation can absorb petroleum hydrocarbon pollutant, to prevent being diffused in the water logging and salt washing, bio surfactant generated by microorganism in the petroleum hydrocarbon degradation bacteria agent can accelerate the digestion of the petroleum hydrocarbon pollutant and further be converted into carbon dioxide and water by the petroleum hydrocarbon degradation bacteria agent biology. The efficiency of the degradative petroleum hydrocarbon pollutant of the invention method is high, and the speed is quick.

The invention relates to a preparation method of floating embedding bacterium agents for in-situ remediation of oil polluted water, specifically relating to a technology for fixing oil degradation bacteria by using expanded graphite, sodium alginate and calcium chloride, absorbing and degrading to purify the petroleum polluted water. The preparation method of the floating embedding bacterium agents comprises the following steps: adding petroleum degrading bacteria liquid into sodium alginate sol containing the expanded graphite, mixing uniformly, dropping into calcium chloride solution and cross-linking overnight at 4 degrees centigrade to obtain the floating embedding bacterium agents fixing hydrocarbon degradation bacteria. The floating embedding bacterium agents prepared by the preparation method has the advantages of high mechanical strength, good floatability and mass transfer performance and high degradation efficiency; the floating embedding bacterium agents can be directly put onto the surface of the petroleum polluted water; the embedding bacterium agents can float on an oil-water interface for long term so as to ensure effective contact between the bacterium agent and the petroleum; the embedding technology increases the concentration of microorganisms, prevents the bacteria influencing by the water using environment, effectively improves the degradation efficiency, recovers and recycles conveniently and has no secondary pollution risk.

The invention discloses a manufacture method for rhamnolipid that includes the following steps: filtering fungus, inoculating into culture medium after taking dipping preprocess to the sample getting from oil field sewage or grease, inoculating on blood agar plate for culturing, selecting single colony, inoculating on blue agar plate for culturing, selecting the colony having blue halo to the bevel for microbial conserving; fermenting the primary screening strain in fermenting culture medium, testing the oil drainage activity, emulsibility and fermenting liquid surface tension, selecting target strain; taking ferment to the selected strain, and optimizing the fermenting culture medium, fermenting condition and strain to gain rhamnolipid fermenting liquid; distilling rhanmolipid raw product from the rhamnolipid fermenting liquid. The invention has high yield, good heavy hydrocarbon degradation effect, and improves the selecting accuracy.

The present invention discloses petroleum hydrocarbon degradation bacteria being suitable for electric field condition and application thereof. Bacillus licheniformis PS5 of the present invention is isolated from petroleum contaminated soil, can degrade petroleum hydrocarbons, and is preserved in the China Type Culture Collection center on July 19, 2011, wherein the preservation registration number is CCTCC No: M 2011261. The bacillus licheniformis PS5 is used for the remediation of contaminated soil. The strain isolated by the invention has high activity under the condition of 0.5-2.0 V / cm field, and can improve removal of alkanes, aromatics, colloids and mixed petroleum hydrocarbon. By applying the electric field, the degradation activity of the bacillus licheniformis PS5 is increased. After 28 days the removal rates for petroleum hydrocarbon single components and mixed petroleum hydrocarbons are increased by 4.19-21.91% and 7.64-23.32%.

The invention provides a method for separating and screening a saline-alkaline-resistant pure bacterial strain degrading petroleum hydrocarbon from oily soil. The method comprises the steps: firstly collecting soil polluted by salinized petroleum hydrocarbon from an oil field, adding the collecting soil according to an amount of 10% into an inorganic salt culture medium which takes crude oil as a unique carbon source, and carrying out enrichment culture in a constant temperature shaker; then inoculating a 10% enriching liquid into a fresh crude oil inorganic culture medium and continuously carrying out habituated culture under a same condition for 4-5 times, wherein the pH, salinity and crude oil content of the culture medium in the domestication process are gradually raised; after diluting the culture liquid after domestication in a gradient manner, coating the culture liquid to a LB-agar panel, after bacterial colonies grow, picking up single colonies in different shapes, and lineating and purifying and separating single bacteria; and finally, spreading the separated single bacteria to a crude oil inorganic salt-agar panel and detecting whether the bacterial colonies grow or not, if so, the bacterial strain is the bacteria degrading petroleum hydrocarbon; determining the petroleum hydrocarbon degradation rate of the single bacterial strain and carrying out 16Sr DNA molecular identification on the bacterial strain so as to determine the species relationship of the bacterial strain.

The invention discloses an immobilized microorganism oil-spilling repairing agent. The immobilized microorganism oil-spilling repairing agent has an embedded microorganism concentration of 5*10<7> to 2*10<8> cell / g, and has carrier ingredients of mussel shell, sodium alginate and CaCl2. The immobilized microorganism oil-spilling repairing agent has a petroleum hydrocarbon degradation rate of 82-94% under the conditions of salinity of 5-55 parts per thousand, a temperature of 10-30 DEG C and a pH value of 6.5-9.0. Compared with free microorganisms, the immobilized microorganism oil-spilling repairing agent has a degradation rate improved by 39-47% and has improved environmental tolerance.

The invention provides a method capable of rapidly selecting and screening surfactant petroleum hydrocarbon degradation pure bacterial strain from oil soil. According to the method capable of rapidly selecting and screening the surfactant hydrocarbon degradation bacteria on the surface of forming material, the oil soil is collected from an oil field and added into a hydrocarbon degradation culture medium with the amount of 5%, wherein crude oil of the hydrocarbon degradation culture medium is a sole carbon source, the content of the crude oil is gradually improved, and an enrichment culture is carried out on a constant temperature shaker. After the enriched bacteria liquid is diluted to a certain degree and coated to be cultured on a blood plate, 1-3d is cultured at the temperature of 30 DEG C, bacteria colony with a clear and large transparent zone edge on the blood plate is taken, after the bacteria colony is cultured in a liquid meat peptone culture medium, the bacteria liquid is dipped through a sterilizing toothpick blunt end and dibbled on an oil plate, 3d is cultured at the temperature of 30 DEG C, bacteria colony with an obvious and large oil biting spot is taken to be vaccinated in a meat peptone inclined surface and stored, after the bacteria colony is cultured with the meat peptone culture medium, the hydrocarbon degradation culture medium and the surfactant culture medium are vaccinated in sequence, 7d and 3d are cultured at the temperature of 30 DEG C, the hydrocarbon degradation capacity and the surfactant capacity are determined, and therefore the surfactant petroleum hydrocarbon degradation bacterial strain can be obtained.

The invention discloses a microbial oil recovery method. By the method, the crude oil production rate of a field test is improved by 12.5 percent. The microbial oil recovery method provided by the invention comprises the following steps of: 1) carrying out pure oxygen aeration on a culture solution of petroleum hydrocarbon degrading bacteria to obtain an injection solution which is subjected to the pure oxygen aeration; 2) mixing the injection solution which is subjected to the pure oxygen aeration in the step 1) and a bacteria solution which contains the petroleum hydrocarbon degrading bacteria to obtain a mixed solution; and 3) injecting the mixed solution which is obtained in the step 2) into an oil reservoir by a production well for oil recovery to obtain crude oil. The content of dissolved oxygen in the injection solution is increased in a mode of carrying out pure oxygen aeration on the injection solution in a storage tank, so the activity of aerobic and / or facultative microbes can be effectively improved, life activity such as growth metabolism of the aerobic and / or facultative microbes is quickened, the degree of hydrocarbon degradation is improved, and the crude oil production can be improved.

The invention provides pseudomonas with the collection number of CGMCC (China General Microbiological Culture Collection Center) No.4793, a microbial agent containing the pseudomonas, and applications of the pseudomonas or the microbial agent in petroleum hydrocarbon degradation, petroleum pollution bioremediation, petroleum polluted soil bioremediation and petroleum polluted water bioremediation. The pseudomonas with the collection number of CGMCC No.4793 and the microbial agent containing the pseudomonas have strong degrading capability for the petroleum hydrocarbon, can be widely applied to petroleum hydrocarbon degradation and petroleum pollution renovation, petroleum polluted soil renovation, petroleum polluted water renovation and the like.

The invention discloses a method for repairing high-concentration oil-polluted soil through mycorrhizal fungi-plant-degradation bacterium, concretely comprising the the steps of: inoculating an arbuscular mycorrhizal fungi inoculant such as Glomus mosseae bacteria or Glomus versiforme bacteria and a mixed inoculant of petroleum hydrocarbon and degradation bacterium liquid in the high-concentration oil-polluted soil and planting mycorrhizal fungi aulophytes such as shamrocks and corns, growing for 90-125 days, and harvesting corn plant bodies with high biomass liveweight and burning in a centralized manner. The method has the advantage of establishing a composite mycorrhizal fungi-plant-degradation bacterium co-metabolism system; on one side, the mycorrhizal fungi and plants form mutually beneficial symbionts so as to help the plants to absorb nutrition and water and promote the growth of the plants so that the stress resistance of the plants to the high-concentration oil-polluted soil; on the other side, the mycorrhizal fungi is used as heterotrophic microorganism and the petroleum hydrocarbon is used as exogenous carbon which is decomposed and utilized; and moreover, nutrients are provided to exogenous petroleum hydrocarbon degradation bacteria through mycorrhizal fungi and the secretions of plant root cells so that the degradation efficiency of the petroleum hydrocarbon is improved.

The invention discloses a highly effective degradation strain JN6 for petroleum hydrocarbons in oily sludge and application thereof, which belong to the technical field of bioremediation. The degradation strain JN6 is preserved in the China General Microbiological Culture Collection Center, the preservation number is CGMCC NO. 14977, and the classification number is Comamonas sp. The strain JN6 with a petroleum hydrocarbon degradation function which is disclosed by the invention is applied to treatment of oily sludge by degrading petroleum hydrocarbons, the degradation rate of the single strain of bacteria on total petroleum hydrocarbon (TPH) in oily sludge is 62.16 percent within 30 days, and the optimum degradation conditions of the bacteria on petroleum pollutants are 15 DEGC to 40 DEGC and the pH value of 7 to 11. The biological method is used in oily sludge remediation technology, and has the advantages of high effectiveness, low cost, environment-friendliness and the like.

The invention discloses a highly effective degradation strain JN4 for petroleum hydrocarbons in oily sludge and application thereof, which belong to the technical field of bioremediation. The degradation strain JN4 is preserved in the China General Microbiological Culture Collection Center, the preservation number is CGMCC NO.14975, and the classification number is Pseudomonas stutzeri. The strainJN4 with a petroleum hydrocarbon degradation function which is disclosed by the invention is applied to treatment of oily sludge by degrading petroleum hydrocarbons. The degradation rate of the bacteria on total petroleum hydrocarbon (TPH) in oily sludge is 71.05 percent within 30 days. The single strain provided by the invention has obvious degradation capability on n-C10 to n-C30, and GC-MS (gas chromatography-mass spectrometry) analysis indicates that the strain has strong degradation capability on cycloalkane naphthane, indane and d-homoandrostane. The optimum degradation conditions of the bacteria on petroleum pollutants are 25 DEGC to 40 DEG C and the pH value of 5 to 11. The biological method is used in oily sludge remediation technology, and has the advantages of high effectiveness, low cost, environment-friendliness and the like.

The invention relates to an immobilized microbial repairing agent for spilled oil and a preparation method thereof. The repairing agent is prepared by embedding microbes with a sodium alginate-CaCl2-biobased active carbon, wherein the concentration of the embedded microbes is 8*10<8> to 6*10<9> cell / g. The preparation method comprises the following steps: mixing biobased active carbon with microbial seed liquid for adsorption so as to from a mixed suspension; adding a sterilized sodium alginate solution with a temperature of 30 to 40 DEG C into the mixed suspension and carrying out uniform mixig so as to obtain a viscous mixed solution; and subjecting the viscous mixed solution to dispersion and balling in a CaCl2 solution and carrying out cross-linking for 12 to 24 h so as to obtain the immobilized microbial repairing agent for spilled oil. The preparation method is simple and easy to operate and mild in reaction conditions; and the prepared immobilized microbial repairing agent is not prone to microbial leakage, has good stability and reusability and high microbe activity and cell volume and has a petroleum hydrocarbon degradation rate of 81 to 95%.

The invention relates to a composite functional petroleum hydrocarbon degradation microbial agent, as well as a preparation method and application thereof. The sludge petroleum degradation microbial agent is prepared from the following effective living bacteria components: phosphate dissolving bacteria, nitrogen fixing bacteria and petroleum hydrocarbon degrading bacteria; the phosphate dissolvingbacteria are pseudomonas fluorescens ECO, and the strain storage number thereof is CGMCC No. 16104; the petroleum hydrocarbon degrading bacteria are bacillus paramycoides 3, and the strain storage number thereof is CGMCC No. 16238. The prepared microbial agent has the advantages that the degradation rate of petroleum in sludge is detected according to a gravimetric method, and the degradation rate of the petroleum-contaminated soil or sludge with the petroleum content of 3-10% in a high salinity environment can reach 21-50% within 30 days; the products obtained after degradation by the microbial agent are CO2 and H2O, and secondary pollution is avoided.

The invention belongs to the field of environmental material preparation, and particularly relates to a preparation method for charcoal-loaded graphite phase carbon nitride composite photocatalyst capable of degrading petroleum hydrocarbon, and application thereof. The preparation method comprises the following steps: cleaning a soybean straw to remove surface impurities, drying, calcining, naturally cooling to the room temperature, taking out a black charcoal solid, smashing the black charcoal solid into black charcoal powder, and drying after acid pickling to obtain the charcoal; mixing thecharcoal and melamine powder, and grinding to obtain the mixture of the charcoal and the melamine; after the mixture of the charcoal and the melamine is subjected to high-temperature calcination for aperiod of time, the mixture is naturally cooled to the room temperature to be taken out; the obtained black gray solid powder is grinded, and a target product is obtained. The charcoal-loaded graphite phase carbon nitride composite photocatalyst is easy in synthesis, has a high specific surface area and high photocatalytic activity; environmental protection efficacy is obvious, and the soil petroleum hydrocarbon degradation effect is ideal.

The invention relates to an oil base drill chip processing method, belongs to the field of environment protection, and particularly belongs to the technical field of drilling waste processing in oil and natural gas industry. The method comprises the following steps of: (1) demulsification: adding demulsifying agents accounting for 0.4 percent to 1.0 percent of the mass of oil base drill chips into the oil base drill chips in a stirring state; (2) cleaning: adding a non-ionic surfactant solution accounting for 10 percent to 30 percent of the mass of the oil base drill chips and an anionic surfactant solution accounting for 20 percent to 30 percent of the mass of the oil base drill chips into the demulsified oil base drill chips in the first step in the stirring state to obtain an oil-water-solid mixture; (3) separation: carrying out centrifugal separation on the mixture in the step (2); and (4) processing by hydrocarbon degradation bacteria: regulating the pH of the separated oil base drill chips and then adding nutrients, nutrient providers, bulking agents and the hydrocarbon degradation bacteria. The method has the advantages that the oil recovery rate is higher than 94 percent; the oil content of the drill chips is less than 0.3 percent; the operation is simple; the equipment investment is low; and reclamation and harmlessness are realized.

A preparation method for an immobilized microbe oil-spill repairing agent is characterized by comprising the following steps: preparing peanut shell-based active carbon; mixing the peanut shell-based active carbon with a seed bacteria liquid to performing adsorption until adsorption is saturated, so as to form a bacteria suspension; adding a sterilized sodium alginate solution with a temperature of 30-40 DEG C into the above bacteria suspension, uniformly mixing, injecting into a CaCl2 solution with a certain concentration by employing an injector, dispersing into balls and performing crosslinking for 12-24 h, so as to obtain immobilized microbe microballoons; and finally flushing the immobilized microbe microballoons with normal saline for 2-4 times to obtain a finished product. According to the preparation method, the biology-based active carbon modified peanut shell is added into a sodium alginate-calcium chloride embedded immobilized carrier for the first time, the mechanical strength and the mass transfer property of the agent are improved, the activity and the degradation efficiency of microbes are improved; also the cost is low, the technology is simple and easy to operate, the reaction conditions are mild, microbes of the prepared repairing agent is not easy to leak, and the prepared repairing agent is good in stability and reusability, has relatively high microbe activity and cell capacity, and has petroleum hydrocarbon degradation rate of 75-92%.

The invention relates to a liquid microorganism agent used in degrading petroleum pollution in the field of petroleum pollution soil biology mending technology. It separately dose fluid culture to the bacteria and fungus with petroleum hydrocarbon degradation capability and then mixes them to prepare for the liquid microorganism agent.

A preparation method for an immobilized microbe oil-spill repairing agent is characterized by comprising the following steps: preparing corncob-based active carbon; mixing the corncob-based active carbon with a seed bacteria liquid to performing adsorption until adsorption is saturated, so as to form a bacteria suspension; adding a sterilized sodium alginate solution with a temperature of 30-40 DEG C into the above bacteria suspension, uniformly mixing, injecting into a CaCl2 solution with a certain concentration by employing an injector, dispersing into balls and performing crosslinking for 12-24 h, so as to obtain immobilized microbe microballoons; and finally flushing the immobilized microbe microballoons with normal saline for 2-4 times to obtain a finished product. According to the preparation method, the biology-based active carbon modified corncob is added into a sodium alginate-calcium chloride embedded immobilized carrier for the first time, the mechanical strength and the mass transfer property of the agent are improved, the activity and the degradation efficiency of microbes are improved; also the cost is low, the technology is simple and easy to operate, the reaction conditions are mild, microbes of the prepared repairing agent is not easy to leak, and the prepared repairing agent is good in stability and reusability, has relatively high microbe activity and cell capacity, and has petroleum hydrocarbon degradation rate of 78-88%.

A method of preventing thermal hydrocarbon degradation deposits on a surface of a gas turbine component, the method includes providing the turbine component comprising the surface configured for contacting a hydrocarbon fluid, wherein the substrate comprises a material having a nominal liquid wettability sufficient to generate, with reference to an oil, a nominal contact angle, disposing a plurality of features on the substrate to form an anti-deposition surface texture, wherein the plurality of features have a size, shape, and orientation selected such that the surface has an effective wettability sufficient to generate, with reference to an oil, an effective contact angle of greater than the nominal contact angle, and the features comprise a width dimension (a), and a spacing dimension (b), wherein the features prevent the hydrocarbon fluid from penetrating into the surface texture and thereby reduce the adhesion of the thermal hydrocarbon deposits to the surface.

The invention discloses high-efficiency degrading bacteria JN5 for petroleum hydrocarbons in oily sludge and its application and belongs to the technical field of bioremediation. The degrading bacteria JN5 are preserved in the China general microbiological culture collection center, have a preservation number of CGMCC NO. 14976 and are classified as Pseudomonas stutzeri. The bacteria JN5 with a petroleum hydrocarbon degradation function are used for treating oily sludge through petroleum hydrocarbon degradation. In 30 days, the bacteria JN8 have the oily sludge total petroleum hydrocarbon (TPH) degradation rate of 73.73%. The bacteria JN5 have significant ability to degrade linear petroleum hydrocarbons of n-C10 to n-C26 based on a single strain. The optimal degradation conditions of the bacteria to petroleum contaminants comprise a temperature of 20-50 DEG C and pH of 7-11. The bioremediation method for oily sludge has the advantages of high efficiency, low cost and environmental friendliness.

The invention discloses a high-density fermenting method of petroleum hydrocarbon degraded bacteria in the biological fermenting technical domain, which is characterized by the following: activating slope bacteria; enlarging the cultured seed liquid; preparing before inoculating; inoculating; supplementing the ferment culture medium batch by batch; adjusting the even growing speed of bacteria; reducing cost; simplifying the operation; producing large amount of petroleum hydrocarbon degraded bacteria within short period; solving the problem of soil pollution and the accident of petroleum pollution.

Method of modeling the biodegradation of hydrocarbons trapped in an oil reservoir or trap through the action of the bacterial population in an underlying aquifer. From data relative to the reservoir studied, concerning the form and the height of the reservoir, the physical characteristics of the porous medium, the thickness of the transition zone between the hydrocarbons and the water, the composition of the hydrocarbons, of the flow of electron acceptors entering the reservoir and data relative to the bacterial population in the aquifer, modeling is performed by discretizing the reservoir by means of a grid wherein the height of each grid cell is the thickness of the transition zone, and the variation, over the height of the reservoir, of the proportion in heavy fractions of hydrocarbons under the effect of the biodegradation is determined by iterative adjustment in each grid cell of the bacterial population to the amount of hydrocarbons available, the pore space available, the amount of electron acceptors present in the reservoir and the degradation capacities of said population. Applications: determination of the composition of oils in a reservoir and notably of the location of the heaviest fractions.

The invention discloses a bioremediation method for petroleum contamination and its special strains. The strains are (Alcaligenes xylosoxydans)H4-1 with the preservation number of CGMCC No.3235. The strains of the present invention has strong petroleum hydrocarbon degradation capability. The strains, inocula and its application method provide theoretical basis for the bioremediation work of the petroleum contamination soil in our country, have great significance for the environment protection work in the national oil field region and bioremediation work for the contamination due to leakage inthe petroleum transport process speed up the progress of the bioremediation work for petroleum contamination and have important economical significance.

The invention discloses a petroleum hydrocarbon degrading bacteria carrier material and the purpose thereof. A preparation method is as follows: 1) cubing the corncobs, rinsing with water, and soaking in an aqueous solution of hydrochloric acid; taking out the corncobs, washing by sterile water to a neutral state, drying, crushing, sieving through a screen of 20 mesh to obtain a corncob powder; cutting soybean straws into segments, rinsing with water, and soaking in an aqueous solution of hydrochloric acid; taking out the soybean straw segments, washing by sterile water to a neutral state, drying, crushing, and sieving through a screen of 20 mesh to obtain a soybean straw powder; and 2) mixing the corncob powder and soybean straw powder according to the ratio of 2:1 to prepare the petroleum hydrocarbon degrading bacteria carrier material. Compared with the traditional carrier materials, the petroleum hydrocarbon degrading bacteria carrier material provided by the invention can significantly reduce repellency water and surface tension of soil, improve water holding capacity and microbial activity of petroleum polluted soil, create good conditions for the hydrocarbon degradation ability of the bacteria, and significantly improve the degradation efficiency.

The invention discloses an efficient degradation strain JN7 of petroleum hydrocarbons in oily sludge and application thereof, and belongs to the technical field of biological restoration. The degradation strain JN7 is preserved in CGMCC (China General Microbiological Culture Collection Center), has the preservation number of CGMCC NO.14978, and has the classification number of Pseudomonas sp.. Thepurpose of the strain JN7 with the petroleum hydrocarbon degradation function is the oily sludge control performed in a petroleum hydrocarbon degradation mode. The degradation rate of the bacterium in 30 days on the TPH in the oily sludge is 50.75 percent; the singe strain of the bacterium has the obvious degradation capability on n-C10 to n-C30 petroleum hydrocarbons; GC-MS analysis shows that the petroleum hydrocarbon substances in oily sludge take oxidization reaction under the effect of the strain to form a great number of phenol and ketone type intermediate products. The bacterium has the most proper degradation conditions of the temperature being 20 to 50 DEG C and the pH value being 7 to 11 on petroleum contaminants. The biological method is used for the restoration technology of the oily sludge, and has the advantages of high efficiency, low cost, environment-friendly effect and the like.

The invention relates to the field of environmental biotechnology, and aims to provide a united degradation method for dehydrated crude in water by petroleum degradation bacterial flora and surfactant. The method comprises specific steps as follows: (1), a petroleum-contaminated soil sample is taken and placed in a conical flask filled with a petroleum hydrocarbon degradation bacterium medium to be cultured for 5 days; part of a culture solution is transferred into a new culture medium, raw crude is added, and culture is continued for 5 days; culture is performed repeatedly for 3 times to obtain a petroleum hydrocarbon degradation bacterial flora; and (2) the culture medium and the petroleum hydrocarbon degradation bacterial flora are taken, dehydrated crude and the surfactant are added in sequence, and the added dehydrated crude is degraded through constant-temperature oscillation culture. According to the united degradation method for the dehydrated crude in the water by the petroleum degradation bacterial flora and the surfactant, environmental harm by the stubborn dehydrated crude can be removed in a thorough, efficient and low-cost manner; a bacterial flora enrichment method is simple, convenient and fast, 80% of raw crude can be degraded in 35 days only by the bacterial flora obtained through the method, and compared with degradation only through bacterial flora, the degradation efficiency is doubled through united application of the bacterial flora and the surfactant.

Method of modeling the biodegradation of hydrocarbons trapped in an oil reservoir or trap through the action of the bacterial population in an underlying aquifer.From data relative to the reservoir studied, concerning the form and the height of the reservoir, the physical characteristics of the porous medium, the thickness of the transition zone between the hydrocarbons and the water, the composition of the hydrocarbons, of the flow of electron acceptors entering the reservoir and data relative to the bacterial population in the aquifer, modeling is performed by discretizing the reservoir by means of a grid wherein the height of each grid cell is the thickness of the transition zone, and the variation, over the height of the reservoir, of the proportion in heavy fractions of hydrocarbons under the effect of the biodegradation is determined by iterative adjustment in each grid cell of the bacterial population to the amount of hydrocarbons available, the pore space available, the amount of electron acceptors present in the reservoir and the degradation capacities of said population.Applications: determination of the composition of oils in a reservoir and notably of the location of the heaviest fractions.