7 results about "Hydrocarbon degradation" patented technology

The application discloses a salt-tolerant composite bacterial community, a preparation method thereof and application of the composite bacterial community in high-salt oil sludge remediation. Halomonas venusta The composite bacterial community comprises salt bacillus Acinetobacter calcoaceticus C. acnes CCTCC KB 20081402, and pseudomonas aeruginosa Pseudomonas aeruginosa CCTCC AB 2010174. The application utilizes H. venusta The Ectoine secretion characteristic of the DSM 4743 provides osmotic pressure protection for non-salt-tolerant strains in the composite bacterial community, a Ectoine secretion-transmission-degradation synergistic mechanism is constructed, and the degradation efficiency of petroleum hydrocarbon in a high-salt environment is greatly improved. Experiments show that the petroleum hydrocarbon degradation rate of the composite bacterial community in high-salt oil sludge with a salt content of 35 g / L can reach 43.12% within 7 days. The application is suitable for oil sludge remediation in high-salt environments such as offshore oil fields and saline-alkali soil petroleum contaminated sites, and has important environmental and economic benefits.

The invention discloses bacillus velezensis BZ capable of degrading petroleum hydrocarbon, the name of the strain is BZ, the classification name is bacillus velezensis, the strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.31766. The invention further discloses a preparation method of the bacillus velezensis BZ capable of degrading petroleum hydrocarbon. The invention discloses an application of bacillus velezensis capable of degrading petroleum hydrocarbon in degradation of petroleum hydrocarbon. The BZ strain provided by the invention can grow by taking petroleum hydrocarbon as a sole carbon source, can tolerate the low temperature of 5 DEG C and salinity with the concentration of 10%, and can quickly degrade the petroleum hydrocarbon, so that the BZ strain can be widely applied to petroleum degradation. In addition, the strain is simple in culture method and high in tolerant petroleum hydrocarbon concentration, and can be directly used for treating petroleum-polluted water and soil.

This invention relates to a petroleum hydrocarbon-degrading bacterium and its application, belonging to the field of microbial technology. To address the technical problems in existing petroleum-contaminated soil bioremediation, such as the scarcity of highly efficient degrading strains, low degradation efficiency, and insufficient capacity to handle complex hydrocarbons, this invention provides a petroleum hydrocarbon-degrading bacterium, *Acinetobacter pylori* AFRC34, with accession number CCTCC No:2026007. This strain can use petroleum hydrocarbon organic matter in diesel fuel as the sole carbon source and grows on inorganic salt culture media. It maintains a high petroleum hydrocarbon degradation rate even at a low temperature of 15℃. In an inorganic salt culture medium containing 10,000 mg / L high-concentration diesel fuel at 37℃, the degradation rate reaches 92.46%. The strain and inoculant provided by this invention can efficiently degrade petroleum hydrocarbon pollutants, and are particularly suitable for in-situ bioremediation in cold regions and heavily contaminated sites.

This invention discloses a method for preparing a dual-emulsion sustained-release agent for remediating petroleum-contaminated groundwater, belonging to the field of petroleum pollution control. The method includes preparing a nutrient matrix, preparing a dual-emulsion nutrient sustained-release agent, and preparing an outer shell structure. The specific steps for preparing the dual-emulsion nutrient sustained-release agent are as follows: Step 1, obtaining an inner aqueous phase W1; Step 2, obtaining an experimental oil phase O; Step 3, stirring to form a W1 / O type emulsion; Step 4, obtaining an outer aqueous phase W2; Step 5, obtaining the dual-emulsion nutrient sustained-release agent. The specific steps for preparing the outer shell structure are as follows: Step 1, heating to form a gelling agent; Step 2, the ratio of the gelling agent to W1 / O / W2 is 5:1; Step 3, water bath heating; Step 4, solution separation; Step 5, obtaining a boric acid solution; Step 6, forming spherical structures in the solution; Step 7, obtaining the dual-emulsion sustained-release agent. Beneficial effects: It can efficiently degrade petroleum hydrocarbons in groundwater, improving the petroleum hydrocarbon degradation effect by more than 40%.

The application provides a method for rapidly degrading petroleum hydrocarbon in high-salt oil-containing wastewater, adopts Pseudomonas Z1 and denitrifying achromobacter M1 for compounding, adds auxiliary liquid to obtain a bacterial agent, the degradation speed of the bacterial agent on petroleum hydrocarbon and the load tolerance of the bacterial agent on COD in wastewater are obviously higher than those of single bacterial species, and the bacterial agent has good salinity adaptability. In actual wastewater biochemical treatment, the bacterial agent can rapidly establish the bacterial community advantage in a small amount and a short time, effectively improves the microbial abundance and diversity in the system, and can achieve a better petroleum hydrocarbon degradation effect in a limited hydraulic retention time in continuous operation.

The invention relates to the technical field of oilfield chemistry, in particular to bacillus subtilis zymophyte liquid, a microbial composite nano bactericide and a preparation method thereof, and the preparation method of the bacillus subtilis zymophyte liquid comprises the following steps: culturing a bacillus subtilis strain in a culture medium to obtain the bacillus subtilis zymophyte liquid; wherein the culture medium is a fermentation culture medium or a hydrocarbon degradation culture medium. Fermentation bacteria liquid of the bacillus subtilis strain contains lipopeptide biosurfactants, can play roles of dispersing agents, stabilizing agents, blocking agents, reducing agents and the like in metal nanometer synthesis, can inhibit excessive growth of nanometer particles and prevent the nanometer particles from being gathered / condensed in colloid synthesis, and has the capacity of inducing tiny changes of the nanometer particles. The microbial composite nano bactericide is prepared on the basis of the zymophyte liquid, AgNPs in the microbial composite nano bactericide is small in particle size and good in dispersity and stability, and harmful florae such as sulfate reducing bacteria in the oil field environment can be well inhibited.

The invention belongs to the crossing field of environmental engineering, soil remediation and ecological restoration, and relates to a petroleum hydrocarbon degrading bacterium immobilized composite material with pH self-buffering and long-acting slow-release functions and application of the petroleum hydrocarbon degrading bacterium immobilized composite material in contaminated soil remediation and plant synergy. According to the composite material disclosed by the invention, iron alginate gel is used for embedding charcoal to form a composite carrier, and a petroleum hydrocarbon degrading flora is specifically fixed. The composite material disclosed by the invention can be used for efficiently degrading petroleum hydrocarbon; organic acid impact in petroleum hydrocarbon degradation can be effectively resisted, and the microenvironment is stabilized; the long-acting slow release of the petroleum hydrocarbon degrading bacteria is realized, and the bacteria become dominant bacterial communities in the later period of remediation; meanwhile, as a slow-release carbon-based fertilizer and an iron source, the carbon-based fertilizer and the iron source cooperate with functional bacteria metabolites, so that the activity of key enzymes such as soil dehydrogenase and FDA hydrolase is remarkably improved, plant growth is promoted, and systematic remediation integrating single degradation to degradation-improvement-growth promotion is achieved. The material is simple and convenient to prepare, and a new technology is provided for green and sustainable remediation of petroleum hydrocarbon pollution.