41 results about "Hydrocarbon pollution" patented technology

The invention discloses a satellite hyperspectral hydrocarbon pollution monitoring and tracing method and system, and the method comprises the steps: obtaining and preprocessing hyperspectral satellite data, defining a potential pollution region ROI and a pure background spectrum library, extracting hydrocarbon pollution spectral features through a direct and indirect method, and achieving the extraction of pollution information through combining with an intelligent model. And finally, dynamic monitoring, tracing and early warning are carried out. The system correspondingly comprises a data acquisition and preprocessing module, an ROI and background spectrum library construction module, a spectrum feature enhancement and extraction module, a hydrocarbon pollution intelligent extraction module, and a dynamic monitoring traceability and early warning and result output module. According to the method, pollution can be directly identified according to spectral characteristics of hydrocarbon molecular bonds, the monitoring range is wide, the cost is low, the efficiency is high, hydrocarbon pollution monitoring and tracing can be achieved, and powerful support is provided for environment supervision and enterprise self-inspection.

Method for rapid decontamination and for making hydrocarbon-contaminated equipment safe for entry (104) by sequencing a cleaning foam or spray, an encapsulating spray or foam, and a dry carrier gas.

The invention discloses a filler for repairing chlorinated hydrocarbon pollution and a method for repairing chlorinated hydrocarbon polluted underground water by using the filler. The filler is prepared from the following components in percentage by mass: 65-85% of coarse sand, 1-10% of magnetite, 1-5% of groundwater polluted by chlorinated hydrocarbon, 1-5% of wheat bran, 1-5% of coarse gypsum, 1-5% of ferrous sulfate and 1-5% of limestone. The raw materials of the filler are industrial finished products, are low in price and are suitable for actual site repair. The method for repairing chlorohydrocarbon polluted underground water through biogeochemical conversion by using the filler does not need high temperature and high pressure, and the reaction condition is mild. The used filler and emulsified vegetable oil have the advantages of high safety and environmental friendliness, and do not cause secondary pollution to the underground environment. The generation and accumulation of incomplete dechlorination intermediate products can be obviously reduced, so that the chlorinated hydrocarbon can be completely dechlorinated.

The invention belongs to the technical field of sewage treatment, and particularly relates to a sulfur conversion driven chlorohydrocarbon polluted underground water treatment method. According to the method, the multifunctional biochar is prepared through high-temperature ammonia gas modification, PS can be activated to oxidize and remove chlorinated hydrocarbon, then sulfide reduction is catalyzed to remove residual chlorinated hydrocarbon, a porous structure of BC provides pollutant adsorption and fixation functions, and after MFBC, PS and sulfate reducing bacteria are sequentially added into a chlorinated hydrocarbon pollution medium, the pollution medium is subjected to high-temperature ammonia gas modification to remove the residual chlorinated hydrocarbon. The sulfur-containing species undergo a cyclic conversion process from PS-sulfate ion-sulfide-elemental sulfur-sulfate ion. In the conversion process, various oxidation and reduction active substances can be used for effective detoxification of chlorinated hydrocarbon, and MFBC and sulfate reducing bacteria play a key role in mediating sulfide conversion. According to the method, the chlorohydrocarbon in the high-permeability medium can be rapidly removed firstly, and then the chlorohydrocarbon slowly released by the low-permeability medium can be slowly removed at low cost. And the method is low-carbon and environment-friendly, has no secondary pollution such as heavy metals and has obvious application advantages.

Techniques are described herein for using a high-pressure reactor to separate clean water from dirty water without filtration and to extract and concentrate contaminants from dirty water for use as a fuel. In particular, techniques and systems are described for separating water from hydrocarbon contaminates, other BTU-laden compounds, and dissolved minerals, while also boiling water and condensing the resulting steam into distilled water. In addition, system in which the described techniques are performed can be used as a high-pressure pump for moving the separated hydrocarbon contaminates forward into other processes, such as a high-pressure reactor or incinerator.

This invention provides a leaching solution and a method for remediating petroleum hydrocarbon-contaminated soil using the same. The leaching solution comprises: anionic surfactant, nonionic surfactant, inorganic additives, and water. The anionic surfactant is selected from at least one of alkylbenzene sulfonate surfactants and fatty alcohol polyoxyethylene ether sulfate surfactants; the nonionic surfactant is selected from at least one of alkyl glycoside surfactants; and the inorganic additives are selected from silicates and metasilicates. This invention, through the combined use of specific types of anionic surfactants, nonionic surfactants, and inorganic additives, produces a leaching solution that is highly efficient, safe, environmentally friendly, and economical, providing technical support for mitigating current petroleum hydrocarbon pollution of soil, particularly for the remediation of petroleum hydrocarbon-contaminated soil around oil fields and oil depots.

The application provides a method for analyzing the migration heterogeneity of chlorinated hydrocarbon pollution in groundwater in the southern mountainous area by combining isotope tracing and distributed sampling, and belongs to the field of environmental science and engineering technology. The method generates and optimizes the layout of sampling points automatically through a geographic information system (GIS), collects multi-medium samples of soil, water and air, realizes accurate determination of the content of pollutants and isotopes based on the global Moran index and by using isotope systems such as chlorinated hydrocarbon carbon isotopes, chlorine isotopes and radon isotopes. The migration parameters, isotope fractionation coefficients and pollution path probabilities are quantitatively analyzed by using a distributed heterogeneity weighted regression and a Bayesian network model, and the spatial variability and uncertainty are comprehensively explained. Finally, the analysis results are integrated on the GIS platform, and the migration heterogeneity distribution, migration hotspots and risk levels are dynamically presented by using a three-dimensional visualization tool, so that scientific support is provided for the prevention and control of chlorinated hydrocarbon pollution and risk assessment in the southern mountainous area.

The present invention relates to a method for remediating hydrocarbon-contaminated soil, comprising the steps of: size-reducing the hydrocarbon-contaminated soil particles; mixing the size-reduced soil particles with water to form a slurry mixture, wherein the water is degassed prior to or during mixing within a processing vessel; subjecting the slurry mixture to ultrasonic treatment to induce acoustic cavitation and dislodge contaminants from the soil particles; separating the cleaned soil particles from the slurry mixture; and washing the cleaned soil particles with clean water to remove contaminants.

The present invention relates to the field of geophysical detection of petroleum hydrocarbon pollution, and is a magnetic resonance random noise reduction system and method suitable for petroleum hydrocarbon pollution detection. The system comprises a petroleum hydrocarbon pollution random noise reduction network, comprising a deep residual denoiser and a classification discriminator, wherein the deep residual denoiser is configured to process a measured random noise-containing signal according to a nonlinear mapping from a random noise-containing signal to random noise to obtain a predicted random noise, and then remove the predicted random noise from the measured random noise-containing signal to obtain a denoised signal; and the classification discriminator is configured to learn the characteristics of a pure signal in a pure signal set, discriminate the denoised signal input to the classification discriminator as a denoised signal or a pure signal, and feed back the classification result of the classification discriminator to the deep residual denoiser. The deep residual denoiser optimizes network parameters based on the feedback, thereby achieving intelligent denoising.

The invention belongs to the field of environmental microbial remediation, and particularly relates to a heavy metal tolerant pseudococcus dehalogenation strain and application thereof in halohydrocarbon pollution remediation. The pseudococcus dehalogenation Y2 strain can still keep efficient reductive dehalogenation activity under the stress of high-concentration heavy metal. The strain is preserved in China General Microbiological Culture Collection Center (CGMCC) in 2023, the address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, the postal code is 100101, and the preservation number is CGMCC No.40795. The invention further discloses a preparation method of the strain. The Y2 strain provided by the invention can overcome the inhibition effect of heavy metals on dehalogenation microorganisms, and provides an important strain resource for in-situ bioremediation of heavy metal and halogenated hydrocarbon combined pollution sites.

The invention discloses a preparation method of attapulgite modified biochar and application of the attapulgite modified biochar in petroleum pollution remediation, and relates to the technical field of environmental geochemistry. The attapulgite modified biochar provided by the invention can keep good adsorption performance on petroleum hydrocarbon in a saline-alkali environment, can be successfully applied to removal of hydrocarbon such as petroleum hydrocarbon, and can gradually break n-alkanes with relatively large carbon atoms to form n-alkanes with relatively small carbon atoms; aromatic hydrocarbon can be subjected to oxidation and ring-opening reaction to generate hydrocarbon with fewer carbon atoms, a petroleum-polluted sample is treated by the attapulgite modified biochar, the adsorption rate of petroleum hydrocarbon in the sample can be greatly improved, and the adsorption rates of the modified biochar BA400 and BA700 on petroleum hydrocarbon pollution in a saline-alkali environment are respectively improved by 17.66% and 26.29% compared with those of BC400 and BC700. Therefore, the attapulgite modified biochar provides a new method for treatment of saline alkali soil polluted by petroleum hydrocarbon.

A method of treating contaminated materials such as oil and gas production waste sludges to recover crude oil hydrocarbons. The method includes the inversion of water-in-oil emulsions, and subsequent separation steps. These may involve the separation and removal of asphaltenes, petroleum waxes and / or solid particles from the crude oil hydrocarbons. The treatment method uses the physical phenomena of hydrodynamic cavitation and hydraulic shock, which produce different effects upon a mixture of water and the contaminated material being treated. These are deployed either as single or combined stage(s) of treatment or as a repeated series of single / combined treatment stages, with or without additional processing operations between each single / combined treatment stage. The method may be implemented with suitable plant including hydrodynamic cavitation units (103, 106) and hydraulic shock units (104, 107), followed by separators (105, 108).

The invention relates to the technical field of environmental remediation, and particularly discloses a method for remedying chlorinated hydrocarbon pollution, a chlorinated hydrocarbon pollution remediation agent and application, and the method comprises the steps that an injection point is arranged on a chlorinated hydrocarbon pollution site; preparing a sodium persulfate solution with the mass concentration of 7.5%-15%, and preparing a mixed solution of ferrous sulfate with the mass concentration of 1.6%-3.2% and sodium pyrophosphate with the mass concentration of 1.25%-2.5%, namely an iron pyrophosphate mixed solution; drilling is carried out according to the set injection points, drilling is carried out to the pollution depth of the chlorinated hydrocarbon pollution site, and a sodium persulfate solution is sprayed from deep to shallow; spraying a ferric pyrophosphate mixed solution in the same manner, after 7-14 days of chemical oxidation remediation, spraying 5-10 L of emulsified vegetable oil, and naturally remediating for 30-35 days. According to the method, the concentration of chlorohydrocarbon pollutants in chlorohydrocarbon polluted site soil and underground water is remarkably reduced.

The invention discloses a soil petroleum hydrocarbon pollution natural attenuation evaluation method based on fluorescent components, which comprises the following steps: acquiring a three-dimensional fluorescence spectrum of soluble organic matters in petroleum hydrocarbon polluted soil to obtain fluorescence intensity of various fluorescent components; according to the difference between the fluorescence intensities of the multiple fluorescence components, whether the petroleum hydrocarbon in the polluted soil can attenuate naturally or not and the intensity of natural attenuation are evaluated; according to the method, the fluorescence spectrum technology with lower cost is adopted, and the fluorescence components of the soluble organic matters in the polluted soil are analyzed, so that the natural attenuation potential of the petroleum hydrocarbon pollution is judged, and the feasibility of the fluorescence method applied to the identification of the petroleum hydrocarbon pollution of the soil is verified; and a support can be provided for soil environment treatment of a petroleum hydrocarbon polluted site.

The invention belongs to the technical field of environmental engineering and pollution ecological restoration, and particularly relates to a biochar-laccase hybrid nanofilm based on interface bonding, a preparation method and application in petroleum hydrocarbon pollution synergistic restoration and plant enhancement. The biochar-laccase hybrid nanofilm comprises biochar and a laccase-inorganic hybrid nanoflower-shaped film, and laccase protein molecules in the laccase-inorganic hybrid nanoflower-shaped film and functional groups on the surface of the biochar are subjected to interface bonding to generate a composite film structure. The immobilized laccase has an ultrahigh specific surface area far exceeding a theoretical addition value, realizes efficient and stable immobilization of laccase, and shows excellent catalytic activity and environmental stability. When applied to petroleum hydrocarbon contaminated soil, the soil remediation agent can strengthen remediation plants such as oilseed rape to efficiently remove petroleum hydrocarbon through biological and non-biological mechanisms, obviously promote plant growth, realize the crossing from single pollution removal to pollution treatment and ecological restoration collaboration, and provide an innovative solution for green and sustainable soil remediation.

The invention provides a hydrocarbon pollution detection method and device by coupling spectrum subtraction and characteristic T detection, and belongs to the technical field of environmental monitoring. The method comprises the following steps: acquiring target near infrared spectrum data of a to-be-detected area, determining corresponding background near infrared spectrum data according to the ground feature type of the to-be-detected area, and determining the target near infrared spectrum data according to the background near infrared spectrum data; the background near infrared spectrum data is reference spectrum data which is the same ground object as the to-be-detected target and is not polluted by hydrocarbons; performing subtraction processing on the target near infrared spectrum data and the background near infrared spectrum data to generate a difference spectrum; selecting a hydrocarbon characteristic absorption wave band and a reference wave band, and carrying out independent sample T inspection on the spectral value of the difference spectrum in the hydrocarbon characteristic absorption wave band and the spectral value of the difference spectrum in the reference wave band; and determining a hydrocarbon pollution detection result of the to-be-detected area based on the detection result. According to the method, background interference is eliminated through spectrum subtraction, an objective judgment standard is established by utilizing T test, and the scientificity and applicability of hydrocarbon pollution detection are improved.

The invention belongs to the technical field of environmental functional materials, and particularly relates to a cobalt-nitrogen modified zero-valent iron ternary nitride material, preparation thereof and application of the cobalt-nitrogen modified zero-valent iron ternary nitride material in removal of chlorinated hydrocarbon. The invention provides a cobalt nitrogen modified zero-valent iron ternary nitride material which can be used for removing chlorinated hydrocarbon in a water body. Zero-valent iron serves as a matrix, cobalt powder, sodium amide and zero-valent iron powder are mixed through a mechanochemical method, ball milling is carried out under the inert atmosphere, and the cobalt-nitrogen synergistic modified ternary nitride structure is formed. The cobalt powder and the sodium amide are doped into the surface and the interior of the zero-valent iron, so that the zero-valent iron generates lattice strain, the chlorohydrocarbon dechlorination capacity is improved, the preparation process is simple, low in cost, free of pollution and suitable for large-scale application, and the problems of low degradation efficiency and poor selectivity in chlorohydrocarbon pollution treatment are solved.

Hydrocarbon contaminant detection system using polymer film includes a sensor assembly, a syringe, a heater assembly and a controller. The sensor assembly includes a cyclic olefin copolymer (COC) film including electrically conductive materials, and can complete an electrical circuit. The COC film can dissolve in a presence of a hydrocarbon contaminant, breaking the electrical circuit. The syringe can carry a lubrication oil sample to be tested using the sensor assembly. The syringe is operable to contact the lubrication oil sample with the COC film of the sensor assembly. The heater assembly can heat the lubrication oil sample before the lubrication oil sample is loaded to the syringe. The controller can perform operations including operating the syringe to flow the heated lubrication oil sample onto the COC film of the sensor assembly at a pre-determined flowrate.

The invention belongs to the technical field of groundwater environmental pollution remediation, and particularly relates to a polydisperse iron-carbon emulsion for removing high-salt and high-ammonia-nitrogen groundwater chlorinated hydrocarbon pollution and a preparation method and application thereof. The polydisperse iron-carbon emulsion with excellent dispersity, stability, rheological property and reaction activity is synthesized by taking polydisperse sulfurized zero-valent iron, biochar and a nano-emulsion as raw materials and combining high-speed shearing and rheological property characterization on the basis of specific process parameters and processes. The polydisperse iron-carbon emulsion can realize efficient removal of chlorinated hydrocarbon pollutants under complex underground water environment conditions such as high salt and high ammonia nitrogen. Meanwhile, the inhibition effect of high ammonia nitrogen on the reaction activity can be effectively resisted, the degradation efficiency on pollutants is effectively guaranteed, and the stability and adaptability of a remediation system under complex hydrochemical conditions are improved.

The application relates to the technical field of repairing devices, in particular to a repairing device and method for treating surface water oil hydrocarbon pollution. The device comprises a base, the outer part of the base is provided with an impeller floating seat, the impeller floating seat is used for providing buoyancy and controlling the movement of the base, a plurality of through grooves are arranged in the side wall of the base, an extension plate is arranged below the through grooves, and an aggregation assembly is arranged at the bottom of the base. When the main gear rotates, the double-head gear set is driven to rotate in meshing mode, the double-head gear set drives the driven gear rod to rotate in meshing mode in the rotating process, the driven gear rod drives the eccentric wheel to rotate coaxially when the driven gear rod rotates, the surface of the base plate is pressed when the eccentric wheel rotates, the sliding rod arranged at the bottom of the base plate slides in the cylinder and extrudes the elastic element, so that the base plate does reciprocating motion in the vertical direction, the modified activated carbon is driven by the base plate to reciprocate on the surface of the surface water, the contact area of the surface of the modified activated carbon and the oil hydrocarbon is increased, and the service life of the modified activated carbon is improved.

This invention discloses a method for remediating halogenated hydrocarbon pollution based on functional plasmid transformation, belonging to the field of environmental microbial remediation technology. The method first extracts plasmid DNA carrying functional genes from organobacteria with reductive dechlorination capabilities (organobacteria with halogen respiration). Then, the functional plasmid DNA and electron donors are directly added to halogenated hydrocarbon-contaminated samples containing indigenous microorganisms to construct a bio-enhanced remediation system. Finally, the system is cultured under anaerobic conditions to transform the functional plasmid into the indigenous microorganisms, thereby endowing or enhancing the indigenous microorganisms with the ability to reductively dechlorinate halogenated hydrocarbon pollutants. This invention avoids the ecological risks of directly introducing exogenous live bacteria. Through plasmid-mediated horizontal gene transfer, it rapidly activates and enhances the function of in-situ microbial communities, significantly improving the degradation rate and thoroughness of halogenated hydrocarbon pollutants, and providing an efficient, safe, and universally applicable technical means for in-situ bioremediation of contaminated sites.

The invention relates to the field of petroleum hydrocarbon pollution geophysical method detection, in particular to a magnetic resonance random noise reduction system and method suitable for petroleum hydrocarbon pollution detection. Comprising a petroleum hydrocarbon pollution random noise reduction network, the petroleum hydrocarbon pollution random noise reduction network comprises a depth residual noise reduction device and a classification discriminator, the depth residual noise reduction device is used for processing and actually measuring a random noise-containing signal according to nonlinear mapping from the random noise-containing signal to the random noise to obtain predicted random noise, and the classification discriminator is used for classifying the predicted random noise. Removing the predicted random noise from the actually measured random noise-containing signal to obtain a de-noised signal; and the classification discriminator is used for learning the characteristics of the pure signals in the pure signal set, discriminating the de-noising signals input to the classification discriminator as de-noising signals or pure signals, and feeding back the classification result of the classification discriminator to the deep residual noise reducer, and the deep residual noise reducer optimizes network parameters according to the feedback, thereby realizing intelligent de-noising.

The present application relates to the field of environmental bioremediation, and discloses a dehalobacter and application of the dehalobacter in remediation of chlorophenol halogenated hydrocarbon pollutants. The dehalobacter is Co4CP (Dehalobacter sp. strain Co4CP), and the strain has been preserved in the China General Microbiological Culture Collection Center (CGMCC) on February 24, 2024, located at No. 1, Xibahe Road, Hua-yuan District, Beijing, China, and the Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, with a preservation number of CGMCC 41183. The strain provides an important strain resource for in-situ remediation of chlorophenol contaminated sites.

The application provides a hydrocarbon pollution detection method and device coupled with spectral difference subtraction and characteristic T test, and belongs to the technical field of environmental monitoring. The method comprises the following steps: obtaining target near-infrared spectrum data of a detection area; determining corresponding background near-infrared spectrum data according to the ground feature type of the detection area, wherein the background near-infrared spectrum data is the reference spectrum data of the same ground feature as the detection target and not polluted by hydrocarbon; performing difference subtraction processing on the target near-infrared spectrum data and the background near-infrared spectrum data to generate a difference spectrum; selecting a hydrocarbon characteristic absorption wave band and a reference wave band, and performing independent sample T test on the spectrum value of the difference spectrum in the hydrocarbon characteristic absorption wave band and the spectrum value in the reference wave band; and determining the hydrocarbon pollution detection result of the detection area based on the test result. The application eliminates background interference through spectral difference subtraction, establishes an objective judgment standard by using T test, and improves the scientificity and applicability of hydrocarbon pollution detection.

The present invention relates to a method for remediating hydrocarbon-contaminated soil, comprising the steps of: size-reducing the hydrocarbon-contaminated soil particles; mixing the size-reduced soil particles with water to form a slurry mixture, wherein the water is degassed prior to or during mixing within a processing vessel; subjecting the slurry mixture to ultrasonic treatment to induce acoustic cavitation and dislodge contaminants from the soil particles; separating the cleaned soil particles from the slurry mixture; and washing the cleaned soil particles with clean water to remove contaminants.

The application discloses a method for detecting a petroleum hydrocarbon soil pollution plume, and is suitable for a polluted site, especially a service site pollution plume sketching. The application realizes preliminary sketching of a petroleum hydrocarbon pollution plume by coupling a high-density resistance method with a portable modular soil gas stratified sampling method. The portable modular soil gas stratified sampling method uses a portable modular soil gas stratified sampling device to perform stratum sampling, and sets an air inlet interval protection device. When the air inlet interval protection device is not opened, the air inlet interval protection device is in a non-breathable state. Only when the air inlet interval protection device is opened, the air inlet interval protection device has air inlet conditions. The method guarantees sampling accuracy, and has the advantages of low price, high efficiency and improved efficiency.

Mixed media tube or sock filters of variable length with mixed media designed to remove heavy metals and hydrocarbon contaminants, inter alia. The filters of the present invention can be used to control sediment in run-off from a construction site, as drain covers, or as retaining members for lawns or planting areas. A number of or a continuous length of such tube filters can be joined together to form assemblies which are useful for protecting a drain, series of drains, or other water mass, for example a pond or riverbank. Such tube filters can be synergistically joined with other environmental protection approaches, including but not limited to wattles such as ERTEC ProWattle https: / / ertecsystems.com / products / perimeter-sediment-control-four-types / prowattle-perimeter-control / , top guards such as Top Guard Drain Inlet Protection https: / / ertecsystems.com / products / top-guard-drain-inlet-protection / , curb guards such as Curb Inlet Guard https: / / ertecsystems.com / products / top-guard-drain-inlet-protection / curb-inlet-guard / , grate guards such as GR8 Guard https: / / ertecsystems.com / products / top-guard-drain-inlet-protection / gr8-guard / , and slot guards such as https: / / ertecsystems.com / products / top-guard-drain-inlet-protection / slot-guard / .

This application discloses a membrane, its preparation method, and its application in natural gas membrane decarbonization. The membrane comprises a base membrane, a hydrophilic layer, and a hydrophilic functional coating. The hydrophilic layer is made of at least one material selected from ethanolamine, diethanolamine, diaminopropanol, diaminobenzoic acid, and 2,4-diaminobenzenesulfonic acid. The functional coating is made of at least one material selected from cellulose acetate, polyethylene glycol, chitosan, polyvinyl alcohol, and polylactic acid. Under heavy hydrocarbon pollution conditions, the swelling of the hydrophilic material is very slight, completely ensuring the integrity of the membrane surface and separation performance. The process is simple, the raw materials are inexpensive, and the reliability is high, exhibiting significant technical advantages.

The present application belongs to the field of environmental microbial remediation, and specifically relates to a heavy metal tolerant dehalobacterium and application thereof in halogenated hydrocarbon pollution remediation. The dehalobacterium Y2 strain can maintain high-efficiency dehalogenation activity under high-concentration heavy metal stress. The strain has been preserved in the China General Microbiological Culture Collection Center (CGMCC) in 2023, address: No. 1, Yihuangyuan, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, postcode: 100101, preservation number: CGMCC No. 40795. The Y2 strain provided by the present application can overcome the inhibitory effect of heavy metals on dehalogenation microorganisms, and provides important strain resources for in-situ bioremediation of heavy metal and halogenated hydrocarbon compound contaminated sites.

The invention relates to the technical field of petroleum hydrocarbon pollution bioremediation, and discloses an application of a microorganism JQ-3 in petroleum degradation, a microorganism-containing composition, and an application and a preparation method of the microorganism-containing composition. According to the invention, the new application of the microorganism JQ-3 in degrading petroleum is found for the first time. A composition containing a microorganism, wherein the composition contains laccase and the microorganism JQ-3; wherein the laccase is shown as a sequence table SEQ. ID. NO: 1. The preparation method of the microorganism-containing composition comprises the following step: mixing a bacterial liquid containing the microorganism JQ-3 and a protein liquid containing the laccase according to a ratio to obtain the microorganism-containing composition. According to the technical scheme, the composition for degrading the petroleum is prepared by combining the specific degrading bacteria and the specific degrading enzyme. When the composition containing the microorganisms provided by the invention is used for degrading petroleum / petroleum pollutants, the degradation capability of the composition is far better than that of independent treatment of bacteria, compound bacteria or enzymes, and the composition containing the microorganisms shows a very good synergistic degradation effect.