The invention discloses a dsRNA
nanoparticle compound for preventing and treating
panonychus citri, a preparation method and application. The compound is prepared from an
acaricide (preferably
etoxazole), a nano carrier (preferably
graphene oxide nanosheets GONs) and dsCPAP3-2 of a targeted epidermal
protein gene CPAP3-2. The dsCPAP3-2 is synthesized through an in-vitro transcription or in-vivo vector expression method, and has a good
drug resistance
treatment effect and industrialization potential. The GONs are prepared by oxidation, activation, modification and purification. The dsRNA nano-particle compound is assembled according to a specific proportion and condition, and through dual mechanisms of
silence of a
target gene by dsCPAP3-2 to cause
ecdysis failure of nymphs and inhibition of
chitin synthetase by
etoxazole, synergistic
mite killing is realized, so that the sensitivity of the
acaricide can be improved, a high effect can be achieved with low dosage, the problems of
drug resistance and poor stability of
biopesticide are solved, and the application prospect is broad. And efficient protection and accurate delivery of
bioactive molecules are realized, and the prevention and
treatment effect and the lasting period are remarkably improved.