92 results about "Acute toxicity" patented technology

The invention discloses a fungal fibrinolytic enzyme and a preparation method thereof. The fungal fibrinolytic enzyme is prepared by taking a Cordyceps militaris strain as a strain and performing liquid fermentation culture, separation and purification. The relative molecular weight of the fibrinolytic enzyme is about 21,000. The Cordyceps militaris fibrinolytic enzyme has good thrombolytic performance, is free from obvious acute toxicity, has prospects for clinical trial, development and application, and adds a new member to a rare fibrinolytic enzyme family. Particularly, a preferable fibrinolytic-enzyme Cordyceps militaris strain C.LSG-1 obtained through severe screening is rough in growth conditions, short in enzyme production cycle and capable of harvesting a large amount of Cordyceps militaris mycelium during enzyme production, and is the strain excellent in production performance. The Cordyceps militaris fibrinolytic enzyme is an extracellular enzyme which is particularly beneficial to subsequent separation and purification during preparation. The fungal fibrinolytic enzyme takes corn protein with extensive sources as a main culture medium, thereby having low cost for raw materials and providing a novel way for developing and utilizing the prior resources.

The invention relates to a method for preparing America roach adult extractive, wherein it comprises that combines alcohol extraction and water extraction techniques, while the total recycle rate can reach 25.2% and the amino acid content of recycled product can reach 58.5%. The inventive method is simple with low cost and short time. The test on rat has proved that the invention has no side effect and toxic.

The invention relates to chiral supramolecular nucleoside hydrogel based on a boron ester bonds as well as a preparation method and application of the chiral supramolecular nucleoside hydrogel, and particularly provides supramolecular hydrogel which is obtained by mixing guanine nucleoside and borate as raw materials in a solvent, wherein the guanine nucleoside is D-guanine nucleoside and/or L-guanine nucleoside. The supramolecular hydrogel has excellent stability, injectability and self-repairability, also has good biocompatibility, does not show obvious acute toxicity in animal bodies, can be degraded in vivo, and can be used as an extracellular matrix for 3D culture of cells. The supramolecular hydrogel provided by the invention has a very good application prospect in the field of tissue engineering as a scaffold material.

The invention discloses antibacterial peptides and application of the antibacterial peptides to preparation of a medicament resisting drug-resistant bacteria. The antibacterial peptides consist of 21 amino acids, wherein the amino acid sequence is shown as SEQIDNO.1. The in-vivo and in-vitro research shows that six kinds of antibacterial peptides have a strong inhibiting and killing action for the mostly clinically-separated drug-resistant bacteria and can remarkably reduce the mortality of a septicemia model caused by the drug-resistant bacteria; and a hemolytic test and an acute local stimulus test results show that six kinds of derivates have no hemolytic test or acute toxic stimulus reaction when the concentration is 2.5mg/mL.

The present invention discloses an inclusion complex containing the panaxsaponin Rg₂ and the preparation method. The inclusion complex of the invention consists of the panaxsaponin Rg₂ and the hydroxypropyl-Beta-cyclodextrin. The preparation method of the present invention is as following. The hydroxypropyl-Beta-cyclodextrin water solution is prepared; the hydroxypropyl-Beta-cyclodextrin solution is kept in the water bath; the extraction powder of the panaxsaponin Rg₂ or the extraction of the liquid-typed Rg₂ dissoluted by the solvent is added before mixing and complex; therefore the liquid inclusion complex is obtained and the inclusion complex after drying. The inclusion complex of the present invention has functions of increasing the heart perfusion and the blood flow, expanding the diameter of the vein and the artery, improving the microcirculation disturbance, improving the myocardial ischemia and anti-thrombosis effect. The present invention can significantly reduce the acute toxicity of the panaxsaponin Rg₂.

The present invention provides a new application of lactobacillus paracausei K56 capable of adjusting gastrointestinal flora balance. The lactobacillus paracasei (lactobacillus paracausei subsp.paracasei) has a preservation number of DSM27447. The strain of the single bacteria has ability to significantly promote growth of intestinal bifidobacteria and lactic acid bacteria, can inhibit desulphovibrio and/or enterobacter, can inhibit helicobacter pylori and/or escherichia-shigella genera, and can tolerate stress environment of simulated gastrointestinal fluid in vitro. Mouse experiments show that the strain has no oral acute toxicity and no antibiotic resistance, is safe and can be used for food processing.

The invention discloses a pharmaceutical composition for treating cardiac failure which is prepared from the following medicinal herbs (by weight ratio): red ginseng 5-12 parts, astragalus root 15-45 parts, black monkshood 5-15 parts, epimedium 8-15 parts, red sage root 10-20 parts, rhizome of Sichuan lovage 5-15 parts, fruit of citron or trifoliate orange 5-15 parts, ophiopogon root 6-15 parts, and lepidium seed 5-15 parts.

The invention relates to a sterilizing composition containing benzothiostrobin and triazole fungicide. The sterilizing composition comprises active ingredients and auxiliary elements of a pesticide preparation, wherein the active ingredients comprise benzothiostrobin and triazole fungicide; the mass ratio of the benzothiostrobin to the triazole fungicide is (1:50)-(50:1); the triazole fungicide is one of tebuconazole, flutriafol, triadimefon, propiconazole and hexaconazole. The benzothiostrobin and the triazole fungicide of the sterilizing composition are two sterilizing agents having different mechanisms and are not subjected to conflicts after being compounded, a remarkable synergistic effect on the resistant bacteria is achieved within a certain scope, the sterilizing composition has a remarkable control effect, is safe and does not damage crops, the acute toxicity on a mammal is reduced after the benzothiostrobin and the triazole fungicide are compounded, and the safety is improved. The field trial shows that the sterilizing composition expands the bacteriocidal spectrum and has special effects on diseases of the crops such as powdery mildew, leaf spot disease, false smut, scab, banded sclerotial blight, damping-off and the like, and the use amount of the sterilizing composition is less.

The invention relates to substituted tetrahydronaphthalene amide compound as shown in a general formula I (in the Specification), pharmaceutically acceptable salt thereof, and a preparation method and application. The substituted tetrahydronaphthalene amide compound can be used as an antitumor drug, the invention also provides a preparation method of compounds similar to the substituted tetrahydronaphthalene amide compound and pharmaceutical compositions containing the compounds, and in vitro and in vivo anti-tumor effect research results and acute toxicity research results. The substituted benzo-cyclic amide compound as the antitumor drug, has better antitumor activity and safety, particularly resists imatinib mesylate resistance tumors, can be applied to curing tumors, such as leukemia, gastrointestinal stromal tumor, lung cancer, colon cancer, ovarian cancer and kidney cancer, is wide in therapeutic window, and as antitumor agent, the compound has high application value in the field of medicine.

The present invention belongs to the field of Chinese medicine technology, and is one kind of medicine preparation for treating hepatic and biliary diseases. The medicine is prepared with Chinese medicinal materials including rhubarb, giant knotwood, ash bark, dried orange peel, curcuma root, haw and cogongrass rhizome and medicinal supplementary material, and through crushing the Chinese medicine material into fine powder, extracting volatile oil, ethanol reflux, decompression concentration of the extracted liquid, vacuum drying to obtain extractum powder, adding supplementary material, mixing, pelletizing and other steps. The medicine is prepared into tablet, capsule or granule. Tests and clinical application show that the medicine has obvious curative effect and no obvious toxic side effect.

The invention discloses a podophyllotoxin preparation formula for external use for the treatment of condyloma acuminatum and genital herpes, a series of formulations and a preparation method, and belongs to the field of pharmacy. The weights or the volumes of all the ingredients of the formula of 100ml of the podophyllotoxin preparation for external use are as follows: 0.5g of podophyllotoxin, 20ml-80ml of main solvent, 1ml-3ml of penetration enhancer 1, 0.5g-2.5g of penetration enhancer 2, 20ml-80ml of cosolvent and 0.03-0.5g of preservative. The podophyllotoxin preparation for external use has the advantages that the types and the amount of excipients and the proportion of main drugs are reasonable, the drug administration is carried out by a liniment, a spray or other formulations which are applicable to the external use, the main drug can be rapidly absorbed by the horny layer and the epidermis, the efficacy is significant, the local toxicity or side effects are small, the skin acute toxicity is very low, and the podophyllotoxin preparation has no irritation and weak allergy and does not hinder the skin normal functions; the podophyllotoxin preparation has no systemic toxicity or side effects, thereby being safe and effective. The preparation prepared by the formula has the advantages of good appearance, moderate viscosity, easy coating, spraying drug administration, feasible process, controllable quality, simple preparation process, and suitability for industrial batch production.

The invention discloses a traditional Chinese medicine composition for treating osteoporosis. The traditional Chinese medicine composition is prepared from the following raw materials: angelica sinensis, wine-processed rhizoma chuanxiong, prepared rehmannia root, cortex acanthopanacis, cassia twig, salt-processed bark of eucommia, radix dipsaci, rhizoma bletillae, sand-scorch-processed drynaria rhizome, fossil fragments, pseudo-ginseng, radix astragali, dangshen, calcined native copper, pawpaw, herba epimedii, rhizoma dioscoreae hypoglaucae, ground beeltle, salt-processed semen cuscutae and salt-processed fructus psoraleae. The traditional Chinese medicine composition provided by the invention is capable of remarkably increasing the bone mineral density and relieving bone pain. The traditional Chinese medicine composition prepared from combination of the multiple components has a remarkable and definite curative effect on osteoporosis treatment and no obvious acute toxicity reaction, does not cause anorexia during treatment and is safe and reliable in a clinical medication process.

The invention discloses a method for predicting acute joint toxicity of three pesticides to photogenic bacteria, which aims to overcome problems that a conventional toxicology acute joint toxicity evaluation technique is large in testing workload and quantitative evaluation and acute joint toxicity action prediction methods are not available. The method for predicting the acute joint toxicity of three pesticides to photogenic bacteria comprises the following steps: (1) performing pretesting, namely confirming the testing concentration of different pesticides in official tests, wherein the step of performing pretesting, namely confirming testing concentrations of different pesticides in official tests comprises the following steps: (1) primarily confirming high, medium and low photogenic bacteria toxicity concentration ranges of a single pesticide; (2) establishing a dosage-effect equation that y is equal to f(x)(x belongs to [C,C']) of the single pesticide; (3) confirming the testing concentrations of different pesticides in the BBD tests; (2) confirming testing schemes through three-factor three-level center combined testing design (BBD); (3) performing official testing, namely, testing the relative light emission inhibition rates of different test groups of photogenic bacteria; and (4) establishing a model to predict the acute joint toxicity of the three pesticides to the photogenic bacteria.

The invention belongs to the technical field of medicine preparation and provides cancer-restraining medicine and the preparation method thereof. The cancer-restraining medicine is mainly made of torikabuto, sarcandra glabra, poria cocos and the like. The cancer-restraining medicine has no toxic or side effect after going through acute toxicity detection and has remarkable retraining curative effect on liver cancer, lung cancer and esophagus cancer and has remarkable tumor resistant effect after going through detection of medicine effect. When the cancer-restraining medicine is prepared, qualified traditional Chinese medicine decoction pieces are used as materials and are processed by methods of freezing, wetting, acidizing, alkalizing, salting, fermenting, steaming, boiling, frying, toasting and the like. After smashed, the qualified traditional Chinese medicine decoction pieces are made into powder or water-bindered pills to be packed and stored in a warehouse. The cancer-restraining medicine has the advantages of being good in medicine market prospect, huge in developing space, rich in medicine resources, simple in operation technology, not large in production investment, and strong in popularization and application values.

The invention relates to a new process for preparing a cinnamon, Radix aconiti lateralis preparata and rehmannia root preparation. The preparation comprises 1-2 parts of cinnamon, 1-2 parts of prepared Radix aconiti lateralis preparata, 8-16 parts of prepared rehmannia root, 4-8 parts of wine-prepared Fructus Corni, 3-6 parts of Cortex Moutan, 4-8 parts of Chinese yam, 3-6 parts of Poria cocos and 3-6 parts of Rhizoma Alismatis. The process has the following advantages: the above medicines are extracted by different solvents respectively according to the main effective components and their physical and chemical properties of the medicines, and a D101 macroporous adsorption resin column is adopted to separate, purify and remove impurities, so the taking amount of the preparation, which is greatly reduced, is 40-85% lower than the taking amount of original cinnamon, Radix aconiti lateralis preparata and rehmannia root preparations, and 87-96% lower than the taking amount of cinnamon, Radix aconiti lateralis preparata and rehmannia root pills. The preparation which adopts a fat-soluble matrix to disperse has the advantages of substantially shortened disintegration dispersion time limit, absorption benefiting after the dispersion of extracts in the matrix, biological utilization degree improvement, and treatment effect improvement. The process overcomes the discomfort of floating in the stomach after the taking of the original cinnamon, Radix aconiti lateralis preparata and rehmannia root preparations, and solves many abuses of the cinnamon, Radix aconiti lateralis preparata and rehmannia root pills prepared through using crude medicines. Animal experiments show that the preparation has a substantial improvement effect on the deficiency of kidney yang, acute toxicity tests show that the cinnamon, Radix aconiti lateralis preparata and rehmannia root preparation is safe in the clinical use, and the quality control substandard of the preparation is greatly improved than that of the Radix aconiti lateralis preparata and rehmannia root pills and original Radix aconiti lateralis preparata and rehmannia root capsules, so the quality stability and the treatment stability of finished products are guaranteed.

The aloe capsule is one kind of health article, and specifically one kind of natural health article. Aloe leaf is deglued, sun dried and ground into powder. Aloe leaf skin powder is compounded with angelica, fleeceflower root, cordate houttuynia, plantain herb and aweto to produce the aloe capsule. The aloe leaf powder acts to prevent other medicine components to pass through stomach and reach intestinal tract or affected part directly fluently, so that the medicine components may be absorbed by human body. Toxic test shows that the aloe capsule has no any toxic side effect, and trial use probes its excellent health care effect.

The invention relates to a small peptide resisting MDR (multiple resistant bacteria). The small peptide is characterized in that the small peptide has the function of resisting clinically isolated MDR. In-vitro activity assay results prove that 11 small peptides have different antibacterial activities for multiple MDR and have the MIC value ranging from 4 mu g/L to 128 mu g/L according to different bacteria. Compared with a mother pattern, the small peptide has enhanced pseudomonas aeruginosa and candida albicans resistant activity. 6 peptides with better antibacterial activity are selected for an in vivo experiment, results prove that the 6 peptides can notably reduce the death rate of a model mouse because of a pseudomonas aeruginosa standard strain and clinical pan-drug-resistant pseudomonas aeruginosa septicemia and have a synergistic effect with polymyxin; no hemolytic reaction is caused when the concentration of 6 small peptides is 10 mg/ml, and the product safety is higher; local stimulation experiment results of rabbit eye conjunctiva show that the small peptide has no acute toxic stimulus reaction.

The invention discloses an application of a Sinkiang salvia officinalis extract in preparation of medicines for preventing and treating diabetic nephropathy. The application has the beneficial effect that the Sinkiang salvia officinalis extract provided by the invention is applied to preparation of the medicines for preventing and treating diabetic nephropathy. An objective and scientific evaluation is made for the safety of total phenolic acid extracts of Sinkiang salvia officinalis through an experiment; a dosage basis is provided for experiment research for preventing and treating DN (diabetic nephropathy); firstly, the median lethal dose (LD50) is determined through an acute peroral toxicity experiment of mice; on this basis, the preventive and therapeutic effect and possible action mechanism of the total phenolic acid extracts of the Sinkiang salvia officinalis on DN are determined through in vivo experiments on animals; an experiment basis is provided for preventing and treating DN employing natural medicines; and technical data are also provided for further development and utilization.

The present invention relates to a Chinese medicine composite for curing primary hypertension. It is made up by using Chinese medicinal materials of raw astragalus root, red ginseng, cinnamon twig, unripe bitter orange, lycium berry, aconite accessory tuber and licorice as raw material through a certain preparation process.

A sorbic acid-tea polyphenol composite nanoparticle and application thereof, the invention relates to the technical field of nanocomposite particle synthesis; its preparation method is as follows: 1. preparing composite nanoparticle; 2. detecting the encapsulation efficiency and Drug loading, the prepared composite nanoparticles have been tested for functional response performance, and qualified composite nanoparticles have been obtained; 3. Safety performance testing of composite nanoparticles: starting from the overall safety of composite nanoparticles, through the hemolytic test , In vitro cytotoxicity test and mouse acute toxicity test to study the in vitro and in vivo toxicity of composite nanoparticles, to initially understand the intensity and nature of their toxicity, and to obtain the dose-effect relationship; 4. Apply composite nanoparticles to the development of fresh-keeping health care. The safety performance of the composite nanoparticles is guaranteed, which provides an important guarantee for later application, and has a wide range of applications, providing a new application for the existing fresh-keeping health care field.

The invention discloses a Chinese medicinal preparation for treating children's diarrhea, which is prepared from dried concrete extracted from eight kinds of Chinese medicinal herbs including purple sweet basil volatile, beta-cyclodextrin inclusion compound of the extracted volatile oil, effervescent agent, aspartame as edulcorant and right amount of soluble starch. The invention also discloses a process for preparing the medicinal preparation for treating children's diarrhea.