The invention discloses a sterilization composition containing dichloro famoxadone and a methoxy acrylate sterilizing agent. The methoxy acrylate sterilizing agent is one of benzothiostrobin, benzene kresoxim-methyl, fluoxastrobin, azoxystrobin, pyraclostrobin, trifloxystrobin or picoxystrobin, and a weight ratio of dichloro famoxadone to the methoxy acrylate sterilizing agent is 20:1-1:20. The sterilization composition can be used for preventing and controlling various diseases like late blight, powdery mildew, downy mildew, banded sclerotial blight, gray mold, brown spot and anthrax on crops. Compared with existing preparations containing dichloro famoxadone or the methoxy acrylate sterilizing agent, the sterilization composition is remarkable in synergistic effect, excellent in sterilization effect, wide in sterilization spectrum, long in acting duration and safe to the crops, pesticide consumption is reduced, pesticide residue on the crops is lowered, environment pollution is reduced, generation of pesticide resistance of pathogenic bacteria is delayed, and service life of pesticide is prolonged.