105 results about "Sheath blight" patented technology

The invention discloses bacillus tequilensis and application thereof, and belongs to the technical field of biology. The preservation number of the bacillus tequilensis is CCTCC (China Center For Type Culture Collection) NO: M 20251909, and the bacillus tequilensis has a remarkable inhibition effect on rice sheath blight disease pathogenic bacteria (Rhizoctonia solani). An in-vitro confrontation culture and potting verification method is adopted to prove that the strain can inhibit the growth of germs, and a plate confrontation experiment shows that the hypha growth inhibition rate of the strain to YM-Q-7 reaches 70.36%. The bacterial suspension (1 * 10 < 8 > CFU / ml) of the strain is used for performing root irrigation treatment on potted rice, the prevention and treatment effect reaches 28.85%, and a good application prospect is provided for prevention and treatment of rice sheath blight disease.

The invention discloses an application of OsPRSBR1 protein and a coding gene thereof in regulation and control of plant sheath blight resistance, and belongs to the technical field of biology. The application of the OsPRSBR1 gene and the protein coded by the OsPRSBR1 gene in regulating and controlling the sheath blight resistance of plants is found for the first time, and a theoretical basis and a research thought are provided for molecular breeding and germplasm resource improvement related to the sheath blight resistance; it is further proved through experiments that after the OsPRSBR1 gene is knocked out, the resistance of the plant to the banded sclerotial blight is weakened, and after the OsPRSBR1 gene is over-expressed, the resistance of the plant to the banded sclerotial blight is enhanced; therefore, the method has a good application prospect in the field of agricultural production.

The invention discloses an application of a rice bHLH transcription factor OsbHLH024 and a coding gene thereof in disease-resistant breeding, and belongs to the technical field of gene engineering. The amino acid sequence of the rice bHLH transcription factor OsbHLH024 is as shown in SEQ ID NO. 2, and the nucleotide sequence of the gene OsbHLH024 is as shown in SEQ ID NO. 1. Experiments prove that through overexpression of the OsbHLH024 gene in the Zhonghua 11, the resistance of rice to rice blast, banded sclerotial blight and leaf blight can be remarkably improved; the OsbHLH024 gene is knocked out, and the resistance of rice to rice blast, banded sclerotial blight and leaf blight can be remarkably reduced. Therefore, the OsbHLH024 gene can positively regulate the disease resistance of the plant, which is of great significance to the cultivation of disease-resistant rice varieties.

The invention belongs to the field of molecular biology, and particularly relates to application of an OsNiR1 gene to improvement of rice broad-spectrum disease resistance and improvement of rice agronomic traits. The invention provides an application of an OsNiR1 gene, and the application comprises an application in preventing and treating plant diseases and / or enhancing the resistance of plants to pathogenic bacteria, and / or an application in improving agronomic traits of the plants. The amino acid sequence coded by the OsNiR1 gene is as shown in SEQ ID NO. 1, and the CDS sequence of the OsNiR1 gene is as shown in SEQ ID NO. 2. The application proves that OsNiR1 participates in regulating and controlling the immune response pathway of rice to fungal disease rice blast, fungal disease banded sclerotial blight and bacterial disease bacterial leaf blight. Meanwhile, after the strong promoter ubi is adopted for driving, an overexpression material of the OsNiR1 is higher and stronger than a wild plant type, and the tiller number is increased. The invention provides important gene resources and molecular theoretical basis for developing a new variety of broad-spectrum disease-resistant rice.

Provided is a use of genes OsADR1 and OsTIR in enhancing broad-spectrum disease resistance in rice. The genes OsADR1 and OsTIR can enhance resistance in rice against diseases such as rice blast and sheath blight, and thus play a valuable role in the development of new disease-resistant rice varieties.

The present application relates to a kind of phenyl-tetrazole oxime derivatives and its preparation method and application, wherein the phenyl-tetrazole oxime derivatives have the structure as shown in formula (I), wherein R 1 , R 2 , T, R a , R b , R c , R d , R e Have the meaning described in the present application;The phenyl-tetrazole oxime derivatives described in the present application have excellent prevention effect on cucumber downy mildew, rice sheath blight, cucumber gray mold, cucumber powdery mildew and the like, and can be further developed as a fungicide for application.

This invention discloses an oxyindene compound containing substituted pyridine and its application as a fungicide. The oxyindene compound containing substituted pyridine has broad-spectrum fungicidal activity, especially effective in controlling rice sheath blight, apple tree rot, rapeseed sclerotinia stem rot, wheat scab, pepper blight, cucumber anthracnose, tomato gray mold, corn curvature blight, rice blast, tomato early blight, wheat take-all, and wheat stem rot. It can be used as a fungicide for the prevention and control of the above-mentioned plant diseases.

This application discloses a Bacillus subtilis strain JTFB-01 and its applications, belonging to the field of microbiology. The Bacillus subtilis strain JTFB-01 has the accession number CGMCC No. 37199 and is deposited at the China General Microbiological Culture Collection Center on December 25, 2025. Field trials show that this inoculant, applied through seed treatment, achieves control effects of 68.20%, 71.73%, and 72.15% against wheat take-all, sheath blight, and root rot, respectively, and significantly increases wheat plant height and yield. It also shows a field control effect of over 50% against cotton Verticillium wilt, with a yield increase of over 17%. This inoculant effectively controls soil-borne diseases and overcomes crop replanting obstacles through multiple mechanisms, including inhibiting pathogens, colonizing the rhizosphere, and promoting growth, thus possessing significant agricultural application value.

The invention discloses an L-carvone hydrazone derivative and an application thereof. The L-carvone hydrazone derivative disclosed by the invention has the advantages of low raw material cost, simple and convenient synthesis route and the like, and the obtained L-carvone hydrazone product has an excellent bacteriostatic effect on rhizoctonia solani, botrytis cinerea, sclerotinia sclerotiorum, fusarium graminearum, valsa mali and phytophthora capsici, and can be used for preventing and treating various plant fungal diseases.

This invention discloses a strain of Streptomyces gravidarum and its application, belonging to the field of biotechnology. The preservation number of the Streptomyces gravidarum is: CCTCC NO: M 20251912. The Streptomyces gravidarum can be used to control rice sheath blight.

The invention relates to the field of plant genetic engineering, and discloses a corn sheath blight disease-resistant gene GRMZM2G057329 and application thereof, and the corn sheath blight disease-resistant gene GRMZM2G057329 comprises any one of the following sequences: a nucleotide sequence shown as SEQ ID No.1, a nucleotide sequence shown as SEQ ID No.2, a nucleotide sequence shown as SEQ ID No.3, a nucleotide sequence shown as SEQ ID No.4, a nucleotide sequence shown as SEQ ID No.5, a nucleotide sequence shown as SEQ ID No.6, a The nucleotide sequence is as shown in SEQ ID No.2; the amino acid sequence of the protein is shown as SEQ ID No.3; the nucleotide sequence has 90% or more of identity with the nucleotide sequence as shown in SEQ ID No. 1 or SEQ ID No. 2 and is used for coding protein with the same function as the nucleotide sequence as shown in SEQ ID No. 1 or SEQ ID No. 2. According to the present invention, the DNA fragment of the complete coding segment of the anti-disease gene GRMZM2G057329 separated and cloned from corn is adopted, and the GRMZM2G057329 gene expression quantity is increased so as to significantly enhance the sheath blight resistance of the corn genetic transformation plant;

The invention provides an alkaloid Waltherione S analogue as well as a synthesis method and application thereof, and relates to the technical field of alkaloid Waltherione compounds. According to the present invention, the structures of the Waltherione A and the Antidesmone are simplified, the pyridine and pyridone alkaloid Waltherione S analogue is designed and synthesized, and the synthesized compound has broad-spectrum bacterial killing activity on banana wilt pathogen, wheat scab pathogen, banana anthracnose pathogen, phytophthora capsici pathogen, botrytis cinerea, rhizoctonia solani, rice blast pathogen, mango stem-end rot pathogen and the like, the compound also has good nematicidal activity on root-knot nematodes, provides a favorable candidate agent for natural, safe and low-toxicity innovative pesticides, and is expected to be applied to agricultural production.

This invention relates to the field of medicinal chemistry, specifically to a capsaicin-like compound containing isopropanolamine, its preparation method, and its application. This type of compound has a structure as shown in general formula (I). The invention uses capsaicin and isopropanolamine as pharmacophores, connecting the two pharmacophores with different bridging chains to synthesize a series of capsaicin-like derivatives containing isopropanolamine. These compounds exhibit excellent inhibitory effects on plant pathogenic bacteria such as rice bacterial blight, citrus canker, rice bacterial leaf streak, and kiwifruit canker; and plant pathogenic fungi such as cucumber gray mold, pepper wilt, rapeseed sclerotinia rot, eggplant verticillium wilt, wheat scab, potato late blight, blueberry root rot, grape coccidioidomyces, dragon fruit anthracnose, and rice sheath blight, providing an important scientific basis for the research and development of new pesticides.

The invention discloses a virens and white beard fungus strain and application thereof, and belongs to the technical field of biology. The preservation number of the white beard virens SJ-50F is CCTCC (China Center For Type Culture Collection) NO: M 20251911, and the white beard virens SJ-50F has a remarkable inhibition effect on a rice sheath blight disease pathogenic bacterium rhizoctonia solani YM-Q-7. According to the invention, an in-vitro confrontation culture and potting verification method is adopted, which proves that the virens SJ-50F can inhibit the growth of germs, a plate confrontation experiment shows that the hypha growth inhibition rate of YM-Q-7 reaches 82.36%, and the hypha growth inhibition rate of fermentation supernate of the virens SJ-50F to YM-Q-7 reaches 90.79%.

The application discloses a kind of compounds with carbonyl coupling rigid fragment and its preparation method and bactericidal purposes.The compound has the structure shown in general formula I.A rigid carbonyl skeleton is constructed by connecting amino-containing 1,2,4-triazole through amide bond, and maleic acid structure and bioactive biological amine fragment are introduced, which are integrated in the same molecular skeleton by carbonyl coupling method.The compound synthesis route is short, the reaction condition is mild, and the yield is high.The biological activity test results show that the compound has broad-spectrum and significant inhibitory activity on rice sheath blight fungus, tomato botrytis cinerea, rice blast fungus, grape anthracnose fungus, oilseed rape sclerotinia sclerotiorum and many other plant pathogenic fungi.The compound has multi-target synergistic mechanism and low risk of drug resistance.

The application discloses a kind of phenylacrylamide derivatives and preparation method and purposes thereof, belong to organic synthesis chemistry and pesticide technical field.Phenylacrylamide derivatives are prepared by condensation reaction from cinnamic acid or its derivatives and benzimidazole derivatives.Biological activity test shows that the compounds exhibit different degrees of inhibitory activity on rice sheath blight pathogen, barley root rot pathogen, wheat scab pathogen and brassica rapa sclerotinia pathogen, and can be used for preparing fungicide for preventing and treating agricultural plant diseases.

The application belongs to the technical field of genetic engineering, and particularly relates to a rice gene OsUSP23 and application of the encoded protein in regulation of disease resistance of rice. Previous studies have found that OsUSP23, as a protein containing a USP domain, is significantly up-regulated when Magnaporthe oryzae invades, which proves that it may be involved in the interaction between rice and Magnaporthe oryzae; after cultivating overexpression OsUSP23 -OX plants, it is found that the disease is reduced and the fungal biomass is reduced, which indicates that the rice significantly enhances the resistance to Magnaporthe oryzae and Rhizoctonia solani; after cultivating knockout mutants osusp23 of plants, the resistance of rice to Magnaporthe oryzae is reduced, which indicates that the gene OsUSP23 positively regulates the resistance of rice to Magnaporthe oryzae; the application effectively reduces the dependence of rice on pesticides by cultivating OsUSP23 -OX disease-resistant varieties, and provides a potential genetic target point for molecular breeding of rice broad-spectrum disease-resistant varieties.

The invention relates to a methylene ester bridge-containing compound as well as a preparation method and a bactericide application thereof. The compound has a structure as shown in a formula (I). The compound disclosed by the invention has broad-spectrum bactericidal activity on various plant fungal diseases, can be used for preventing and treating various plant fungal diseases independently or by being compounded with other medicaments, and particularly has an excellent preventing and treating effect on rice sheath blight disease, cucumber gray mold, wheat scab and sclerotinia rot of colza.

The invention provides indole C3-O functionalized derivatives as well as a preparation method and insecticidal and antibacterial application thereof, and relates to the technical field of pesticide development. The indole C3-O functionalized derivative provided by the invention has a structure of a general formula (1), is prepared through a free radical cyclization reaction catalyzed by N-heterocyclic carbene (NHC), does not need a transition metal catalyst, and efficiently constructs a C3-site oxygen functional group by taking o-nitroalkyne or cis-o-nitrostyrene and aldehyde as raw materials through multi-step series conversion in an inert atmosphere. The method has the advantages of being high in atom economy, good in functional group compatibility, easy and convenient to operate, environmentally friendly and the like. The obtained derivative shows remarkable activity in prevention and treatment of various agricultural diseases and insect pests such as rice sheath blight disease, aflatoxin and brown planthopper, can be prepared into various pesticide preparations, has the characteristics of broad spectrum, high efficiency and low toxicity, and is suitable for development of green pesticides.

This invention discloses a soybean lecithin-based microbial agent for disease prevention and root promotion, its preparation method, and its application, belonging to the field of microbial technology. The agent comprises a microbial ensemble, modified enzymatically hydrolyzed soybean lecithin, a carrier, and auxiliary components. This invention utilizes the synergistic effect of modified enzymatically hydrolyzed soybean lecithin and the microbial ensemble. The modified enzymatically hydrolyzed soybean lecithin undergoes pretreatment, stepwise low-temperature treatment, enzymatic hydrolysis, and enzyme inactivation and purification steps, improving its quality and performance. This enhances the colonization rate of the strain in the rhizosphere, improving not only disease prevention and root promotion effects but also improving the soil microecological environment, creating favorable growth conditions for plant roots, and further enhancing the disease prevention and root promotion effects of the agent. The agent of this invention significantly reduces the incidence of wheat sheath blight, reduces the use of chemical pesticides, and can also be applied to the cultivation of other crops, playing a role in the prevention and control of various plant diseases and promoting root growth.

The invention discloses a formononetin derivative containing piperidine-4-carbohydrazide as well as a preparation method and application of the formononetin derivative, and belongs to the technical field of pesticide synthesis. According to the invention, a piperidine-4-carbohydrazide group with excellent activity is introduced into the structure of formononetin, a series of formononetin derivatives of piperidine-4-carbohydrazide are synthesized, and the activity of the synthesized formononetin derivatives containing piperidine-4-carbohydrazide in inhibiting plant pathogens is tested. It is found that the formononetin derivative of piperidine-4-carbohydrazide synthesized by the invention can effectively inhibit plant pathogenic fungi, especially rhizoctonia solani.

The application discloses a rice anti-Rhizoctonia solani leptin receptor OsPLS3 protein and an encoding protein and application thereof. The rice leptin receptor OsPLS3 protein has an amino acid sequence as shown in SEQ ID NO. 3. A coding gene of the rice leptin receptor gene OsPLS3 has a genomic nucleotide sequence as shown in SEQ ID NO. 1, and an open reading frame nucleotide sequence of the rice leptin receptor gene OsPLS3 is as shown in SEQ ID NO. 2. Experiments prove that overexpression of the rice leptin receptor gene OsPLS3 in the application in rice can improve the resistance of the rice to the Rhizoctonia solani, and the rice is more susceptible to the Rhizoctonia solani after knockout, namely, the OsPLS3 positively regulates the resistance of the rice to the Rhizoctonia solani. The rice leptin receptor gene OsPLS3 has important production value for breeding of the rice resistant to the Rhizoctonia solani.

The invention relates to application of a polycyclic quinazolinone compound in resisting plant pathogenic fungi in the technical field of organic synthetic chemistry and agricultural pharmacology, the structural formula of the derivative is shown as I or II, in the structural formula, R is H, 4-OMe, 4-Me, 4-CO2Me, 4-F, 4-CF3, 4-Cl, 4-Br, 2-Br or 2-F, and R is H, 4-OMe, 4-Me, 4-CO2Me, 4-F, 4-CF3, 4-Cl, 4-Br, 2-Br or 2-F; the series of compounds have a certain inhibition effect on plant pathogenic fungi including strawberry botrytis cinerea, fusarium graminearum, rhizoctonia solani and magnaporthe oryzae, and can be applied to plant protection as a bactericide. The preparation method comprises the following steps: by taking N-cyanamide olefin as a raw material and lauroyl peroxide as an oxidizing agent, reacting in a dichloromethane or trichloromethane solvent at 80-120 DEG C to obtain the polycyclic quinazolinone compound with antibacterial activity. The preparation method is simple to operate, green and efficient.

The invention provides a heterocyclic ring-containing nicotinamide compound, a synthesis method and application of the heterocyclic ring-containing nicotinamide compound in prevention and treatment of plant fungal diseases, and relates to the technical field of pesticides. The heterocyclic ring-containing nicotinamide compound is synthesized by using easily available raw materials and a simple, practical and low-cost synthesis method. The compound has a synergistic effect on rhizoctonia solani, magnaporthe oryzae, mango anthracnose pathogen, rubber anthracnose pathogen, litchi anthracnose pathogen, banana anthracnose pathogen, coffee anthracnose pathogen, fusarium graminearum, wheat powdery mildew pathogen, valsa mali, botrytis cinerea, sclerotinia sclerotiorum, sigatoka musae, banana scab pathogen, apple ring spot pathogen, botrytis cinerea, botrytis cinerea, botrytis cinerea, botrytis cinerea and botrytis cinerea. The compound has an excellent inhibiting effect on plant fungal diseases caused by fungi such as tobacco alternaria alternata, cotton rhizoctonia solani, exserohilum turcicum and hevea brasiliensis, is novel in structure, and has huge potential for creation of novel pesticides.

The invention provides application of an OsCML36 gene in improving resistance of rice to sheath blight disease, and belongs to the technical field of gene engineering. The overexpression rice OsCML36 gene is applied to any one of the following applications: (1) the resistance of rice to sheath blight is improved; (2) the agronomic traits of the rice are changed; (3) cultivating transgenic rice with strong sheath blight resistance; (4) cultivating transgenic rice with changed agronomic characters; the nucleotide sequence of the rice OsCML36 gene is as shown in SEQ ID NO. 1. The application of the OsCML36 gene in regulating and controlling the resistance and agronomic traits of the rice sheath blight disease has important significance in analyzing the disease-resistant mechanism of the gene and cultivating rice plants with high disease resistance and high quality.

The invention belongs to the field of pesticides, and discloses a compound with a structure as shown in a formula I. A preparation method of the compound comprises the following steps: reacting anthranilic acid with formamide, and then reacting with 1, 4-dibromobutane under the action of sodium hydride to obtain 3-(4-bromobutyl) quinazoline-4 (3H)-ketone; then, phenylacetic acid sequentially reacts with methyl alcohol, hydrazine hydrate, potassium hydroxide, carbon disulfide and 3-(4-bromobutyl) quinazoline-4 (3H)-ketone, and the quinazolinone derivative containing the oxadiazole unit is obtained. The quinazolinone derivative containing the oxadiazole unit is a novel compound, has an inhibiting effect on fusarium graminearum, rhizoctonia solani and botrytis cinerea, and can be used for preventing and treating plant diseases caused by fungi.

The invention discloses a gene type3AF for regulating and controlling the resistance of rice sheath blight disease as well as an encoding protein and application of the gene type3AF. The CDS sequence of the gene type3AF is as shown in SEQ ID NO.1, the full-length sequence of the gene type3AF is as shown in SEQ ID NO.2, and the protein sequence coded by the gene type3AF for regulating and controlling the rice sheath blight disease resistance is as shown in SEQ ID NO.3. According to the invention, a new rice sheath blight disease resistance negative regulation gene type3AF is successfully identified and obtained; two different banded sclerotial blight resistance identification methods verify that the knockout line of the type3AF gene in a rice plant can significantly improve the banded sclerotial blight resistance of the plant, and has no significant influence on main agronomic traits; however, overexpression of the type3AF gene in rice does not change the sheath blight resistance, but leads to significant changes in rice plant height and flag leaf length, and the type3AF gene has the research of breeding application value. The type3AF gene provided by the invention can provide a new resource for excavation of sheath blight resistant rice genes, provides a core target for cultivation of sheath blight resistant rice varieties, and has an important application value for maintenance of grain production safety.

The present application relates to the technical field of pesticide compounding, and discloses a fungicidal composition containing allylazole and thiazolamide, wherein the weight ratio of the allylazole to the thiazolamide is 1:50-50:1. The pesticide composition is mainly used for preventing and treating rice sheath blight, tobacco brown spot and tomato early blight. Since the two components have different action mechanisms, the composition has obvious synergistic effect, can effectively reduce the use amount of pesticides, and has good economic and social benefits in popularization and application, and helps to realize the reduction and synergism of pesticides and meet the friendly trend of national green environmental protection.

The application discloses a fungicidal composition, which comprises imazalil and phenoxazine-1-carboxylic acid with a mass ratio of 0.5-5:1, or the fungicidal composition comprises imazalil and phenoxazine-1-carboxylic acid with a mass ratio of 1:3.4-5.5. The fungicidal composition has a synergistic effect on the prevention and treatment of rice sheath blight, strawberry gray mold or pepper anthracnose.