261 results about "Sheath blight" patented technology

The invention discloses application of an LRK20 gene in regulation and control of rice disease resistance, a related biological material and a cultivation method. According to the application of the LRK20 gene in regulation and control of rice disease resistance, the sequence of the LRK20 gene is shown as SEQ ID NO.1, and the disease resistance is sheath blight resistance. The amino acid sequence of the protein coded by the LRK20 gene is as shown in SEQ ID NO. 2. The recombinant vector comprises the complementary sequence of the LRK20 gene in the application. The novel gene LRK20 for regulating and controlling the resistance of the rice sheath blight disease is obtained through identification, the sheath blight resistance gene LRK20 can regulate and control the resistance of the rice sheath blight disease, the resistance of the rice to the sheath blight disease can be remarkably weakened by knocking out the gene, and the resistance of the rice to the sheath blight disease can be remarkably enhanced by overexpressing the gene; therefore, the gene has a potential breeding application value in sheath blight resistance molecular breeding.

The invention discloses and relates to a strain capable of resisting saline and alkaline and producing IAA (Indoleacetic Acid). The strain is classified and named as bacillus atrophaeus, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) NO.29799. The strain is preserved in China General Microbiological Culture Collection Center on January 29, 2024. The bacillus atrophaeus HHQGTS13-1 can grow under the condition that the salt content is higher than 3%, can generate indoleacetic acid, cellulase, amylase, protease and ligninase, can inhibit various plant pathogenic bacteria, such as banana anthracnose, tomato gray mold, cotton verticillium wilt, rice sheath blight disease, wheat head blight, banana fusarium wilt, capsicum white spot and the like, and can be used for preparing the bacillus atrophaeus HHQGTS13-1. Particularly, the compound plays an important role in preventing and treating wheat scab, banana anthracnose and capsicum white spot. The strain can promote plant growth in a saline-alkali environment, and can be used for improving soil quality, developing special microbial fertilizers, microbial agents and biopesticides for saline-alkali soil, relieving crop salt stress damage and increasing crop yield.

The invention discloses a gene LRK21 for regulating and controlling the resistance of rice sheath blight disease as well as an encoding protein and application thereof, and belongs to the technical field of plant genetic engineering. The novel gene LRK21 for regulating and controlling the resistance of the rice sheath blight disease is identified, and the identification result of the resistance of the sheath blight disease of in-vitro leaf sheaths in a climatic chamber proves that the resistance of rice to the sheath blight disease can be weakened by knocking out the gene LRK21, and the resistance of the rice sheath blight disease can be reduced by overexpression of the gene LRK21. The result shows that the LRK21 gene participates in the regulation and control of the resistance of the rice sheath blight disease, and the disorder of the function of the LRK21 can influence the resistance of the rice to the sheath blight disease, so that an important gene resource is provided for the research on the resistance mechanism of the rice sheath blight disease.

The invention belongs to the field of molecular biology, and particularly relates to application of OsDOF3 protein or a coding gene thereof to improvement of rice broad-spectrum disease resistance and regulation of agronomic traits. According to the application, an OsDOF3 knockout mutant and an overexpression transgenic line are constructed under the background of susceptible rice, resistance phenotype analysis is performed by inoculating various pathogenic bacteria (including pyricularia oryzae, rhizoctonia solani and bacterial blight), and it is proved that OsDOF3 participates in regulation and control of an immunoreaction pathway of rice to fungal diseases (rice blast and sheath blight) and bacterial diseases (bacterial blight). Meanwhile, the application also finds that after a strong promoter ubi is adopted for driving, an overexpression material of the OsDOF3 is shorter than that of a wild plant, and the OsDOF3 influences normal growth and development of rice while stimulating plant immune response. The invention provides important gene resources and molecular theoretical basis for developing a new variety of broad-spectrum disease-resistant rice.

The invention relates to the technical field of pesticides, in particular to a validamycin / thifluzamide double-loaded preparation with an acid-base response characteristic. The preparation method comprises the following steps: firstly, loading thifluzamide by using calcium carbonate to form a microsphere structure, and simultaneously loading validamycin and thifluzamide calcium carbonate microspheres by using sodium carboxymethyl cellulose and sodium alginate as carrier materials to construct the double-load pesticide preparation. The validamycin / thifluzamide double-loaded preparation provided by the invention has the characteristic of responding to the pH value of the environment, and can simultaneously realize quick release of validamycin and long-acting release of thifluzamide, so that the pesticide effect is remarkably improved. Besides, the validamycin / thifluzamide double-loaded preparation provided by the invention has good leaf surface wettability and release performance, has a relatively good prevention effect on rice sheath blight disease, and has relatively high safety to aquatic organisms represented by zebra fish.

The invention relates to the technical field of plant genetic breeding, in particular to a rice sheath blight resistance related gene and an encoding protein and application thereof. The invention provides a rice sheath blight resistance related gene as well as an encoding protein and application thereof, the gene is OsSBR4 or a related gene thereof, the resistance of rice to sheath blight can be remarkably enhanced by knocking out the gene, and the resistance of rice to sheath blight can be reduced by overexpressing the gene. After agronomic traits of a transgenic line of the gene are investigated, it is found that the plant height, the ear length, the ear number, the thousand seed weight and the like are not affected, and it is indicated that the gene has important application value in sheath blight resistance molecular breeding. According to the invention, a novel rice sheath blight resistance related gene is screened and excavated, and the identification of the sheath blight resistance gene can provide a theory and a basis for breeding a sheath blight resistance rice variety.

According to the nano pesticide and the preparation method and application thereof, pH response type nano particles ZIF-90 serve as a carrier, azoxystrobin is loaded to serve as a medicine, and the nano pesticide is used for preventing and treating rice sheath blight disease caused by rhizoctonia solani. When the rhizoctonia solani infects rice, oxalic acid can be generated to reduce the pH around an infected area, so that the ZIF-90 carrier skeleton and the hyaluronic acid coating are decomposed to release azoxystrobin, and the rhizoctonia solani is inhibited in a targeted manner. It is further found that ZIF-90 and azoxystrobin have synergistic bacteriostatic activity, the EC50 value of azoxystrobin to rhizoctonia solani can be greatly reduced, after ZIF-90 carrier encapsulation and hyaluronic acid end capping, the photolysis stability of azoxystrobin can be improved, the adhesive force of azoxystrobin on rice leaves can be enhanced, and the ZIF-90 and azoxystrobin compound has important application in agricultural disease control.

The invention relates to the technical field of microbial soil remediation, in particular to a microbial composition for remediation of saline alkali soil, a microbial agent and application of the microbial agent. According to the characteristics of the bacillus subtilis KC000 and the paenibacillus mucilaginosus JDY001, different compounding proportions are set and applied to the saline-alkali soil, then the salt content of the saline-alkali soil is measured, and it is found that the soil nutrition and environmental conditions are obviously improved by applying the microbial agent, and the saline-alkali soil can be applied to the saline-alkali soil. The soil conditioner is beneficial to the growth and reproduction of paenibacillus mucilaginosus and bacillus subtilis in soil, is beneficial to improving the content of organic matters, rapidly available phosphorus and rapidly available potassium in the soil, increases the content of alkali-hydrolyzable nitrogen in the soil to a certain extent, reduces the pH value of the soil, accelerates salt leaching, inhibits salt return and reduces the salt content of the surface layer of the soil. Meanwhile, the microbial composition also has the effect of preventing and treating sheath blight.

The invention belongs to the technical field of microorganisms, and particularly discloses trichoderma harzianum NBU-1 and application thereof. The Trichoderma harzianum NBU-1 is preserved in the Guangdong Microbial Culture Collection Center on March 26, 2025, the preservation address is the 5th floor, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou, and the preservation number is GDMCCNO: 66053. The invention discloses trichoderma harzianum NBU-1 and application thereof, the trichoderma harzianum NBU-1 is applied to prevention and treatment of wheat sharp eyespot, and the activity of rhizoctonia cerealis R0301 and the growth of scab are effectively inhibited.

According to the suspending agent containing the pydiflumetofen and the meconazole, provided by the invention, the defects of a traditional single bactericide are overcome, and the control effect on various plant diseases is improved. According to the suspending agent disclosed by the invention, a natural biological source substance chitosan oligosaccharide is introduced as a thickening agent, so that the prevention and treatment effect of the agent on the rice sheath blight disease can be improved unexpectedly.

The present invention belongs to the field of microbial application technology, and specifically relates to a Streptomyces torulosus for preventing and controlling plant diseases and its application. The Streptomyces torulosus is Streptomyces torulosus Jna201, with a deposit number of CGMCC No. 34097, and was deposited at the General Microbiology Center of the China National Committee for the Collection of Microorganisms on April 3, 2025. The present invention provides a Streptomyces torulosus for preventing and controlling rice sheath blight and corn stalk rot. The present invention shows through greenhouse efficacy experiments that Streptomyces torulosus Jna201 can effectively inhibit the lesion length of rice sheath blight and corn stalk rot in greenhouse efficacy determinations, indicating that Streptomyces torulosus Jna201 can effectively inhibit the infection of rice sheath blight and corn stalk rot, and has important significance for the green prevention and control of rice sheath blight and corn stalk rot.

The invention discloses bacillus tequilensis and application thereof, and belongs to the technical field of biology. The preservation number of the bacillus tequilensis is CCTCC (China Center For Type Culture Collection) NO: M 20251909, and the bacillus tequilensis has a remarkable inhibition effect on rice sheath blight disease pathogenic bacteria (Rhizoctonia solani). An in-vitro confrontation culture and potting verification method is adopted to prove that the strain can inhibit the growth of germs, and a plate confrontation experiment shows that the hypha growth inhibition rate of the strain to YM-Q-7 reaches 70.36%. The bacterial suspension (1 * 10 < 8 > CFU / ml) of the strain is used for performing root irrigation treatment on potted rice, the prevention and treatment effect reaches 28.85%, and a good application prospect is provided for prevention and treatment of rice sheath blight disease.

The invention discloses an application of OsPRSBR1 protein and a coding gene thereof in regulation and control of plant sheath blight resistance, and belongs to the technical field of biology. The application of the OsPRSBR1 gene and the protein coded by the OsPRSBR1 gene in regulating and controlling the sheath blight resistance of plants is found for the first time, and a theoretical basis and a research thought are provided for molecular breeding and germplasm resource improvement related to the sheath blight resistance; it is further proved through experiments that after the OsPRSBR1 gene is knocked out, the resistance of the plant to the banded sclerotial blight is weakened, and after the OsPRSBR1 gene is over-expressed, the resistance of the plant to the banded sclerotial blight is enhanced; therefore, the method has a good application prospect in the field of agricultural production.

The invention discloses an 8-bromine-5-substituted-1, 3, 4, 5-tetrahydrothiapyran [4, 3-b] indole derivative, and the 8-bromine-5-substituted-1, 3, 4, 5-tetrahydrothiapyran [4, 3-b] indole derivative is 8-bromine-5-(4-chlorophenethyl)-1, 3, 4, 5-tetrahydrothiapyran [4, 3-b] indole and / or 8-bromine-5-phenethyl-1, 3, 4, 5-tetrahydrothiapyran [4, 3-b] indole. The invention further discloses a preparation method of the 8-bromine-5-substituted-1, 3, 4, 5-tetrahydrothiapyran [4, 3-b] indole derivative. The 8-bromo-5-substituted-1, 3, 4, 5-tetrahydrothiapyran [4, 3-b] indole derivative provided by the invention has the effects of inhibiting rhizoctonia solani and promoting seed germination. The compound provided by the invention not only can be used for preventing and treating rice sheath blight disease through spraying treatment, but also can be used for preventing rice seeds from carrying bacteria in a seed soaking manner, so that the rice sheath blight disease is comprehensively prevented and controlled. Besides, rice seeds are soaked in a compound solution, germination of the rice seeds can be promoted, the root length, growth vigor and fresh weight indexes of the seeds are improved, and the compound has good application prospects and value in prevention and treatment of the rice sheath blight disease.

The invention discloses an application of a rice bHLH transcription factor OsbHLH024 and a coding gene thereof in disease-resistant breeding, and belongs to the technical field of gene engineering. The amino acid sequence of the rice bHLH transcription factor OsbHLH024 is as shown in SEQ ID NO. 2, and the nucleotide sequence of the gene OsbHLH024 is as shown in SEQ ID NO. 1. Experiments prove that through overexpression of the OsbHLH024 gene in the Zhonghua 11, the resistance of rice to rice blast, banded sclerotial blight and leaf blight can be remarkably improved; the OsbHLH024 gene is knocked out, and the resistance of rice to rice blast, banded sclerotial blight and leaf blight can be remarkably reduced. Therefore, the OsbHLH024 gene can positively regulate the disease resistance of the plant, which is of great significance to the cultivation of disease-resistant rice varieties.

The invention provides a nutrient solution and application thereof in prevention and treatment of crop sheath blight, and belongs to the technical field of rice planting. The nutrient solution provided by the invention contains cysteine with a certain concentration, after the nutrient solution is applied to rice in the trefoil stage and cultivated to the whole growth period, the resistance of the rice to sheath blight can be improved, and meanwhile, the content of chlorophyll, soluble sugar, soluble protein, SOD, POD, MDA and CAT in rice plants is remarkably increased. The nutrient solution provided by the invention can effectively solve the problem of poor sheath blight disease resistance of rice under conventional planting conditions, and has a wide application prospect.

The invention belongs to the field of molecular biology, and particularly relates to application of an OsNiR1 gene to improvement of rice broad-spectrum disease resistance and improvement of rice agronomic traits. The invention provides an application of an OsNiR1 gene, and the application comprises an application in preventing and treating plant diseases and / or enhancing the resistance of plants to pathogenic bacteria, and / or an application in improving agronomic traits of the plants. The amino acid sequence coded by the OsNiR1 gene is as shown in SEQ ID NO. 1, and the CDS sequence of the OsNiR1 gene is as shown in SEQ ID NO. 2. The application proves that OsNiR1 participates in regulating and controlling the immune response pathway of rice to fungal disease rice blast, fungal disease banded sclerotial blight and bacterial disease bacterial leaf blight. Meanwhile, after the strong promoter ubi is adopted for driving, an overexpression material of the OsNiR1 is higher and stronger than a wild plant type, and the tiller number is increased. The invention provides important gene resources and molecular theoretical basis for developing a new variety of broad-spectrum disease-resistant rice.

Provided is a use of genes OsADR1 and OsTIR in enhancing broad-spectrum disease resistance in rice. The genes OsADR1 and OsTIR can enhance resistance in rice against diseases such as rice blast and sheath blight, and thus play a valuable role in the development of new disease-resistant rice varieties.

The present application relates to a kind of phenyl-tetrazole oxime derivatives and its preparation method and application, wherein the phenyl-tetrazole oxime derivatives have the structure as shown in formula (I), wherein R 1 , R 2 , T, R a , R b , R c , R d , R e Have the meaning described in the present application;The phenyl-tetrazole oxime derivatives described in the present application have excellent prevention effect on cucumber downy mildew, rice sheath blight, cucumber gray mold, cucumber powdery mildew and the like, and can be further developed as a fungicide for application.

This invention discloses an oxyindene compound containing substituted pyridine and its application as a fungicide. The oxyindene compound containing substituted pyridine has broad-spectrum fungicidal activity, especially effective in controlling rice sheath blight, apple tree rot, rapeseed sclerotinia stem rot, wheat scab, pepper blight, cucumber anthracnose, tomato gray mold, corn curvature blight, rice blast, tomato early blight, wheat take-all, and wheat stem rot. It can be used as a fungicide for the prevention and control of the above-mentioned plant diseases.

The invention discloses a bis (N-acylamino-3, 1-benzoxazinyl) methane compound and a preparation method and application thereof.The compound takes an arylamine compound and bromoacetyl bromide as raw materials, triethylamine as an acid-binding agent and dichloromethane as a solvent to react in an ice-water bath to obtain an N-aryl-2-bromoacetyl arylamine compound, then the N-aryl-2-bromoacetyl arylamine compound is subjected to multi-step reaction, and the bis (N-acylamino-3, 1-benzoxazinyl) methane compound is obtained. Finally, the bis (N-acylamino-3, 1-benzoxazinyl) methane compound with bactericidal activity is obtained. The raw materials for synthesis of the compound are cheap and easy to obtain, and the synthesis method is simple. Meanwhile, the compound has a good inhibition effect on the activity of crop germs, especially has remarkable inhibition activity on sclerotinia sclerotiorum, phytophthora, gibberellic disease bacteria, magnaporthe oryzae, botrytis cinerea and rhizoctonia solani, and has remarkable application value in the aspects of crop disease control and drug resistance challenge.

The invention discloses a rice fork head related structural domain protein coding gene OsFHA11 and application thereof, and belongs to the technical field of plant genetic engineering. According to the invention, the OsFHA11 gene is connected with an overexpression vector through a genetic engineering means and then is transformed into rice, so that the expression of the gene in the transformed rice plant is improved, and the resistance of the rice plant to sheath blight can be improved. Through cloning of the OsFHA11 gene and analysis of the sheath blight resistance function of the OsFHA11 gene, analysis of a molecular mechanism of rice sheath blight resistance is facilitated. In practice, a sheath blight resistant rice material can be cultivated by using the gene through a transgenic means, and a sheath blight resistant rice variety with stable characters is further obtained through interspecies hybridization, so that the occurrence of sheath blight is effectively controlled, and the rice yield is increased.

The application discloses a rice disease resistance related gene OsbZIP65 and application thereof, and belongs to the technical field of plant genetic engineering.The application finds that OsbZIP65 negatively regulates the resistance of rice to sheath blight by constructing an overexpression rice strain and a gene editing rice strain of OsbZIP65 shown in SEQ ID NO.1.The application further finds that a 86bp base sequence in the 5'UTR region of the OsbZIP65 promoter is a core region responding to YWK196 induction of the sheath blight bacteria, the region is differentiated in different haplotypes, and the haplotype is related to the low pathogen-induced expression amount of OsbZIP65 and the enhanced resistance to the sheath blight.Therefore, the disease resistance of rice can be improved by genetic manipulation or haplotype hybridization of the OsbZIP65 gene, and the materials and products of the rice sheath blight resistance can be bred and created, and the disease resistance of rice can be further improved.

This application discloses a Bacillus subtilis strain JTFB-01 and its applications, belonging to the field of microbiology. The Bacillus subtilis strain JTFB-01 has the accession number CGMCC No. 37199 and is deposited at the China General Microbiological Culture Collection Center on December 25, 2025. Field trials show that this inoculant, applied through seed treatment, achieves control effects of 68.20%, 71.73%, and 72.15% against wheat take-all, sheath blight, and root rot, respectively, and significantly increases wheat plant height and yield. It also shows a field control effect of over 50% against cotton Verticillium wilt, with a yield increase of over 17%. This inoculant effectively controls soil-borne diseases and overcomes crop replanting obstacles through multiple mechanisms, including inhibiting pathogens, colonizing the rhizosphere, and promoting growth, thus possessing significant agricultural application value.

The invention discloses an L-carvone hydrazone derivative and an application thereof. The L-carvone hydrazone derivative disclosed by the invention has the advantages of low raw material cost, simple and convenient synthesis route and the like, and the obtained L-carvone hydrazone product has an excellent bacteriostatic effect on rhizoctonia solani, botrytis cinerea, sclerotinia sclerotiorum, fusarium graminearum, valsa mali and phytophthora capsici, and can be used for preventing and treating various plant fungal diseases.

This invention discloses a strain of Streptomyces gravidarum and its application, belonging to the field of biotechnology. The preservation number of the Streptomyces gravidarum is: CCTCC NO: M 20251912. The Streptomyces gravidarum can be used to control rice sheath blight.

The application provides a streptomyces LZZY-S3 and application thereof, and belongs to the field of microorganisms.The streptomyces LZZY-S3 provided by the application enriches strains for biological control, and realizes the dual functions of plant resistance to pathogenic bacteria and plant promotion.The streptomyces LZZY-S3 has the function of inhibiting fungi in a wide range, and can be applied to plant resistance to pathogenic bacteria, and the pathogenic bacteria include Exobasidium vexans, Botryosphaeria spp., Gibberella, Fusarium oxysporum, Pythium, Fusarium graminearum, Didymella, Fusarium pseudograminearum, Trichothecium roseum, Coniella diplodiella, Colletotrichum graminicola or Rhizoctonia cerealis, wherein the inhibition rate of Exobasidium vexans reaches 91.2%.The streptomyces LZZY-S3 can also be applied to plant promotion, preparation of nitrogen-fixing bacterial agents and preparation of ACC deaminase bacterial agents and the like.

The invention relates to the field of plant genetic engineering, and discloses a corn sheath blight disease-resistant gene GRMZM2G057329 and application thereof, and the corn sheath blight disease-resistant gene GRMZM2G057329 comprises any one of the following sequences: a nucleotide sequence shown as SEQ ID No.1, a nucleotide sequence shown as SEQ ID No.2, a nucleotide sequence shown as SEQ ID No.3, a nucleotide sequence shown as SEQ ID No.4, a nucleotide sequence shown as SEQ ID No.5, a nucleotide sequence shown as SEQ ID No.6, a The nucleotide sequence is as shown in SEQ ID No.2; the amino acid sequence of the protein is shown as SEQ ID No.3; the nucleotide sequence has 90% or more of identity with the nucleotide sequence as shown in SEQ ID No. 1 or SEQ ID No. 2 and is used for coding protein with the same function as the nucleotide sequence as shown in SEQ ID No. 1 or SEQ ID No. 2. According to the present invention, the DNA fragment of the complete coding segment of the anti-disease gene GRMZM2G057329 separated and cloned from corn is adopted, and the GRMZM2G057329 gene expression quantity is increased so as to significantly enhance the sheath blight resistance of the corn genetic transformation plant;

The invention provides an alkaloid Waltherione S analogue as well as a synthesis method and application thereof, and relates to the technical field of alkaloid Waltherione compounds. According to the present invention, the structures of the Waltherione A and the Antidesmone are simplified, the pyridine and pyridone alkaloid Waltherione S analogue is designed and synthesized, and the synthesized compound has broad-spectrum bacterial killing activity on banana wilt pathogen, wheat scab pathogen, banana anthracnose pathogen, phytophthora capsici pathogen, botrytis cinerea, rhizoctonia solani, rice blast pathogen, mango stem-end rot pathogen and the like, the compound also has good nematicidal activity on root-knot nematodes, provides a favorable candidate agent for natural, safe and low-toxicity innovative pesticides, and is expected to be applied to agricultural production.