48 results about "Core gene" patented technology

The present invention relates to a gene fusion mutation library construction method, a gene fusion mutation detection method, a gene fusion mutation detection device, computer equipment and a computerstorage medium. The above-mentioned gene fusion mutation library construction method and the gene fusion mutation detection method and device are based on a DNA probe hybridization capture multi-geneRNA targeted sequencing technology, through a fusion gene capture probe hybridization capture target fusion gene, the gene fusion mutation library is constructed, the library can be used for high-throughput sequencing and bioinformatics analysis can identify core genes and partner genes there of constitute breakpoints. Furthermore, the present invention also designs a quantitative analysis methodof the fusion gene, a mutation ratio of the fusion gene can be obtained by calculation, accurate expression value of the fusion gene can be further obtained, and the quantitative analysis method of the fusion gene is pioneering and solves a quantitative analysis problem of using a NGS method to detect the fusion gene.

This invention provides a method for predicting a gene cluster including secondary metabolism-related genes with high accuracy, independent of information concerning core genes. Such method comprises: a step of identifying a region the gene arrangement of which is conserved in nucleotide sequence information of another genome as a gene cluster on the basis of the results of homology search conducted with the use of nucleotide sequence information of at least a pair of genomes; and a step of determining whether or not the gene cluster of interest includes secondary metabolism-related gems on the basis of the proportion of synteny-like regions within the gene cluster identified by the above step.

The invention discloses a rapid detection method for bacteroides based on high-throughput sequencing and application, and belongs to the technical field of molecular biology. A section of core gene fragment rpsD gene with high resolution is obtained through a molecular biology method to serve as a screening marker, high-throughput identification of the bacteroides can be achieved, the compositionof the bacteroides in a complex sample can be comprehensively identified without separation and purification, and the resolution is higher than that of a 16S rRNA gene fragment. A target strip expanded through a specific primer based on the rpsD gene is about 500bp, and compared with a traditional identification method, the identification cost of the bacteroides is saved by 50% or above. The designed primer is combined with high-throughput sequencing, the distribution of the bacteroides in a feces sample can be accurately identified, all reported bacteroides can be identified, and the identification accuracy rate of the bacteroides reaches 100%.

The invention provides a method, a system and a device for establishing a traditional Chinese medicine action mechanism model based on network pharmacology. The method comprises the following steps: acquiring gene targets corresponding to various components of the traditional Chinese medicine; acquiring a target gene target; taking an intersection of the gene targets corresponding to the components of the traditional Chinese medicine and the target gene targets to obtain a gene set; constructing a protein interaction network for the gene set; analyzing nodes in the protein interaction networkto obtain key gene nodes; performing GO analysis and KEGG analysis on the key gene nodes for further gene screening; in the screening result, conducting molecular docking on the traditional Chinese medicine components and protein corresponding to the core gene, verifying the binding capacity, and obtaining the target traditional Chinese medicine components. According to the scheme, efficient drugcomponent analysis can be assisted, the analysis process is simplified, the complexity of an analysis algorithm is reduced, convenience and rapidness are achieved, and the accuracy is high.

The invention relates to the technical field of bioengineering, in particular to application of a KDM3B gene in mesenchymal stem cell osteogenic / odontogenic differentiation, proliferation and migration chemotactic processes. It is found that the KDM3B gene has the effect of positively regulating the function of the mesenchymal stem cells, a basis is provided for application of KDM3B in the processof promoting osteogenic / odontogenic differentiation and proliferation and migration chemotaxis of the mesenchymal stem cells, and a pathway for mediating significant changes of the function of the differentially expressed gene is enriched based on a KEGG database. 14 significant pathways related to differential gene expression are identified in total, and the pathways play a key role in functionregulation of KDM3B. An interaction library based on remarkably regulated GOs and pathways is constructed through signal network analysis, so that 12 core genes are identified according to the degreeof gene interaction, and an important theoretical basis is laid for studying the reconstruction and regulation mechanism of tooth root regeneration seed cells.

The nucleotide and deduced amino acid sequences of cDNAs encoding the envelope 1 genes and core genes of isolates of hepatitis C virus (HCV) are disclosed. The invention relates to the oligonucleotides, peptides and recombinant envelope 1 and core proteins derived from these sequences and their use in diagnostic methods and vaccines.

The invention belongs to the technical field of biological medicines, and particularly relates to a core gene chip and a probe suitable for general survey of colon cancer, and the core gene chip and the probe are used for solving the technical problem of low detection accuracy rate in the prior art. The core gene chip suitable for general survey of colon cancer comprises a solid phase carrier and a group of probes fixed on the solid phase carrier, the group of probes comprises nucleotide sequences shown by SEQ ID NO:1-SEQ ID NO:24 in the sequence table; the core gene probe suitable for general survey of colon cancer by the gene chip is characterized by comprising the nucleotide sequences shown by SEQ ID NO:1-SEQ ID NO:24 in the sequence table. The core gene chip and the probe have the advantages that firstly, early diagnosis of colon cancer is realized, the survival rate of cancerous person is greatly improved; secondly, due to the promotion of the chip technology, the general survey level of the colon cancer is improved to molecular diagnosis from clinical physical examination, so that the general survey level and quality are greatly improved; thirdly, for the promotion of chip molecular diagnosis technology in general survey of colon cancer, passive general survey of the cancer is replaced by active prediction and early treatment.

The invention discloses application of a small molecule compound in preparation of drugs for resisting baculovirus infection of silkworms. The involved small molecule compound is obtained on the basisof a mainstream molecular docking technology, for a necessary core gene of the baculovirus infection of the silkworms, a three-dimensional structure of the core gene is obtained by a virtual modelingtechnology, a small molecule database is screened by molecular docking, the obtained small molecule compound is subjected to activity detection, and finally, the small molecule compound with effectsin the invention is obtained finally. When the using fine concentraton of the small molecule compound is 1 mu g / mL or above, copying of BmNPV can be inhibited completely, after cell infection by BmNPV, copying of BmNPV can be still inhibited, and a therapy effect is achieved. The small molecule compound can be used for researching and producing drugs for preventing and treating nuclear polyhedrosis of the silkworms, and the small molecule compound has application and promotion value.

This invention openly carrying a foreign gene active recombinant protein carrier core area PEAII-HphA and preparation methods and application, but also take the initiative to open a foreign gene carrying the recombinant protein carrier core gene fragment PEAII-HphA ; The initiative delivery exogenous gene's reorganization protein carrier is by guidance member - PEA toxin cross membrane indexing area PEAII- ultra is addicted to the hot Archaea histone HphA composition protein; Under the guidance member's guidance, on the carrier and the target cell's acceptor specificity union has manifested its target tropism, lies between in the acceptor leads passes PEA IIThe cross membrane transportation enters in cell's to swallow the function traversing cell membrane on own initiative, has manifested its initiative, raised the gene transportation efficiency effectively . HphA combination of DNA, and a strong ability with the exogenous gene complexes formed in the active transport carrier in the process, arrived at the exogenous gene, increasing its expression efficiency, cell non-toxic, more secure; Superior to the traditional vector and liposomes,it can be widely used in gene therapy and transgenic animal research.

The invention belongs to the technical field of plant molecular identification, and particularly relates to a strain identification method based on a dunaliella core gene sequence. The method mainly comprises the following steps: collecting, purifying and culturing a sample; extracting the DNA of the whole genome; constructing a DNA sequencing library; obtaining whole genome sequencing data of thealgae strain to be detected and the Dunaliella Quartolecta; screening and de novo assembly of sequencing fragments of the D. Quartolecta core genome are carried out, and genome segmentation, proteinfunction annotation and genome contig colinearity analysis are carried out on the assembled core genome sequence; constructing a phylogenetic tree by utilizing single nucleotide polymorphism, and whenan algae strain to be detected and Dunaliella Quartolecta are gathered into a cluster, the data support rate of branches is 0.99-1.00, the genetic similarity percentage is greater than or equal to 99%, and the algae strain to be detected is D.quartolecta.

Method for inducing TH1 type immune response by inoculating HBs DNA vaccine through using HBV core protein reinforced gene gun, wherein co-leading / co-expression HBV Core gene is used as adjuvant simultaneously while inoculating plasmid HBs DNA vaccine with a gene gun. The animal immunity experiment shows that, the invention can be applied to reinforce the TH1 type immune response level induced through gene gun vaccinated HBs DNA vaccine, including total IgG, IgG2a, specific CTL activity, and antigen specific IFN-gamma production capacity.

The invention provides a primer group and a kit for simultaneously amplifying 13 human STR gene loci and application of the primer group and the kit, and belongs to the technical field of molecular genetics. In the invention, the 13 STR gene loci comprise 12 autosomal STR gene loci and one sex identification STR gene locus. According to the method, 13 STR gene loci with fragments smaller than 320 bp can be amplified in one reaction at the same time, 8 core gene loci and 5 preferable gene loci specified by the Ministry of Public Security are included, meanwhile, a sex identification gene locus is further included, and the problem that detection of trace and degraded large-fragment gene loci of a detected material is lost can be effectively prevented. The 13 STR gene loci are combined with mainstream kits in the market, and more gene locus information can be obtained for trace and degraded DNA, so that the individual recognition capability is effectively improved.

The present invention relates to a method for identifying and classifying microorganisms included in a sample by using an exact k-mer matching algorithm and a bacterial core gene and, preferably, can more quickly and more accurately analyze the taxonomic composition of a metagenomic sample without bias.

The invention discloses application of a bladder urothelium carcinoma detection combined marker, and belongs to the technical field of molecular medicine. The bladder urothelium carcinoma detection combined marker comprises the following gene combinations: TERT, TP53, FGFR3, PIK3CA and ARID1A. According to the invention, it is found for the first time that a combination of five genes of TERT, TP53, FGFR3, PIK3CA and ARID1A can accurately diagnose bladder urothelial carcinoma. Compared with an original method, the method has the advantages that the five core gene detection combinations reduce the sequencing workload by 40 times, and good diagnostic performance is maintained.

The invention discloses a gene code-based material management method and system. The method comprises the steps that gene codes of materials are generated according to core genes and preorder extension genes contained in a material gene code generation request, the gene codes are used for orderly circulation in all service links of a material life cycle, and the core genes are used for representing core features of the materials; the preorder extension gene is used for representing the service characteristics of the material obtained in the initial service link of the material life cycle; an initial material genome is formed by the gene code, the core gene and the preorder extension gene; and service characteristics marked with the gene code is obtained from a non-initial service link of a material life cycle, the service characteristics are compared with a material gene pool to obtain a subsequent expansion gene, and the subsequent expansion gene is accumulated to a current material genome containing the gene code. Unification of material codes of all service links of a material life cycle can be achieved, and co-construction and sharing of material management data are realized.

The invention provides a primer group and a kit for simultaneously amplifying 34 human STR gene loci and application of the primer group and the kit, and belongs to the technical field of molecular genetics. In the invention, the 34 STR gene loci comprise 29 autosomal STR gene loci, one Y chromosome STR gene locus and 4 sex identification STR gene loci. According to the invention, 34 STR gene loci can be simultaneously amplified in one reaction, 20 core gene loci and 10 preferable gene loci specified by Ministry of Public Security are included, all loci of mainstream kits in the current market are included, and meanwhile, 5 gender identification gene loci are also included; and the risk of gender identification errors caused by Y chromosome deletion can be effectively prevented. 34 STR gene loci are combined, so that the method has the characteristics of high individual recognition capability and high non-father exclusion rate.

The invention belongs to the technical field of biological information determination, and discloses a biological information determination method and system, a storage medium, and a terminal. The method comprises the following steps: screening of differentially-expressed genes of pancreatic cancer; GO analysis of the differentially-expressed genes of pancreatic cancer: respectively carrying out GOannotation and graphical display on the obtained differentially-expressed up-regulation and down-regulation genes; KEGG analysis of the differentially-expressed genes of pancreatic cancer: respectively carrying out pathway enrichment and network diagram display on the differentially-expressed up-regulation and down-regulation genes; and PPI interaction network analysis of the differentially-expressed genes and core genes of pancreatic cancer to determine a signal transduction pathway of the differentially-expressed genes. According to the invention, a bioinformatics means is adopted to screenthe differentially-expressed genes of pancreatic cancer and a signal transduction pathway of the differentially-expressed genes, and a molecular mechanism of the occurrence and development of pancreatic cancer is disclosed, so a theoretical basis is provided for early diagnosis and prevention of pancreatic cancer, and an effective target is provided for treatment of pancreatic cancer.

The invention discloses an application of a small molecular compound in the preparation of silkworm antiviral drug. The present invention relates to obtaining small molecular compounds based on mainstream molecular docking techniques, Aiming at the core gene of Bombyx mori baculovirus, the three-dimensional structure of the core gene is obtained by using the virtual modeling technology, the molecular docking screening small molecular database is used for detecting the activity of the obtained small molecular compound, and the small molecular compound with effective effect in the invention is finally obtained. At a final concentration of 1 [mu]g / mL or more, that small molecule compound can completely inhibit replication of BmNPV, and can continue to inhibit replication of BmNPV aft BmNPV completes cell invasion, and has therapeutic effect. The invention can be used for the research and development and production of medicines for preventing and treating blood type pus of silkworm, and has application and popularization value.

The invention discloses a construction method of a lipid metabolism related endometrial cancer (UCEC) prognosis model. According to the construction method, differential genes are screened out layer by layer by using transcriptome data of UCEC in a public database TCGA to construct a prognosis model of lipid metabolism related UCEC, and it is verified that the prognosis model has good predictive capacity for endometrial cancer patients. The prognosis model can be used for guiding clinical endometrial cancer molecular typing and individualized treatment strategies, and products related to endometrial cancer diagnosis, treatment and prognosis can be further developed according to the core gene in the prognosis model.

The invention discloses a construction method of a metabolism-related endometrial cancer (UCEC) prognosis model. According to the construction method, differential genes are screened out layer by layer by using transcriptome data of UCEC in a public database TCGA to construct a prognosis model of metabolism-related UCEC, and it is verified that the prognosis model has good predictive capacity for endometrial cancer patients. The prognosis model can be used for guiding clinical endometrial cancer molecular typing and individualized treatment strategies, and products related to endometrial cancer diagnosis, treatment and prognosis can be further developed according to the core gene in the prognosis model.

The invention discloses a fusobacterium nucleatum related oral epithelial cell tumor biomarker and screening application. The biomarker comprises 15 lncRNAs (long non-coding RNAs) such as AC118757.1,AL162727.1 and AC090844.3. During screening, the human immortalized oral epithelial cells HIOECs are resuscitated and cultured; a fusobacterium nucleatum model strain ATCC25586 is resuscitated; an in-vitro model of the fusobacterium nucleatum infected HIOECs cells is constructed; total RNA of HIOECs infected by fusobacterium nucleatum and uninfected HIOECs cells are extracted respectively to carryout high-throughput sequencing on lncRNA and mRNA transcriptomes, differentially expressed mRNA and lncRNA are screened to further determine a core gene; and the lncRNA participating in differentialexpression of a core gene is screened by constructing an lncRNA-core gene co-expression network. An analysis result can lay a good foundation for further research of fusobacterium nucleatum in the process of affecting malignant transformation and tumors.

The invention discloses a method for constructing a core gene reference short sequence set of pathogenic bacteria, and the method comprises the following steps: determining a reference short sequence of each core gene to establish a core gene reference short sequence, wherein a certain number of reference types are selected from all types corresponding to the core gene to form a reference type set, the nucleotide sequence of each reference type is decomposed into a series of short sequences with fixed base length, and the series of short sequences with fixed base length are the reference short sequence set of the core gene. The invention further discloses a system based on the method and a sample pathogenic bacterium typing method and system based on the method. According to the method and the system provided by the invention, the calculation efficiency and the system robustness can be improved, and private genotype data can be prevented from being uploaded to an uncontrollable public database.

The invention relates to the field of the animal biomedical engineering and specifically discloses a preparation method of recombinant pichia pastoris for surface display of porcine epidemic diarrhea virus core antigen COE protein. A yeast alpha-agglutinin surface display system is adopted to express the antigen core gene, namely COE gene, of the PEDV, and the COE protein is anchored on the cell walls of the yeast, and therefore, the preparation method of recombinant pichia pastoris for surface display of porcine epidemic diarrhea virus core antigen COE protein is provided. The recombinant pichia pastoris can be prepared into novel vaccine products having excellent industrialization prospect; the popularization and application of the vaccines play a positive role in preventing viral diarrhea having serious harm to the pig industry and have great practical significance for protecting and promoting healthy development of the pig industry, and meanwhile, remarkable social and economic benefits can be achieved, and therefore, the vaccines have vast market and industrialization prospect.

The invention discloses a material gene code generation method and system. The method comprises the steps that in response to a received gene code generation request of a coding object, whether a material gene matched with a core gene contained in the request exists in a material gene pool or not and whether a gene matched with a preorder extension gene contained in the request exists or not are judged, the core gene is used for representing the core feature of the coding object, the preorder extension gene is used for representing service characteristics acquired from a material life cycle initial business link of the coding object; if yes, a gene code of the coding object is generated according to the core gene and the preorder extension gene contained in the request, and the gene code is used for orderly circulation in each service link of the life cycle of the coding object. Unification of material codes of all service links of a material life cycle can be achieved, so that data sharing of material management is achieved.

The invention relates to a method for parsing biological genome information through low-cost assembling of a core genome based on apparent group information and application of the method. The novel method for obtaining the core genome sequence information includes the steps of capturing one or more nucleotide sequences used for regulating and controlling gene activity and combined with apparent modification, and conducting database creating, sequencing, splicing, assembling and the like on the obtained sequences to obtain sequence information. By means of the method, gene region sequences or regulation and control region sequences of long segments can be obtained from the species without reference genomes and the species with large genetic difference without depending on any existing sequence information.

The invention discloses a multi-resistant high-quality Qingmaye type red-orange-core Chinese cabbage breeding method. Through the invention, a high-pile heading type TuMV antigen 322 is created by use of the TuMV antigen BP007 and TuMV antigen 826, a selfing line group 1 is created through small hybrid 50, and a self-incompatible line 05-514 is created by use of autumn green 55; hybrid screening is performed by use of the 322, group 1 and 05-514 as well as various red-orange-core materials, the required red-orange-core gene is led into the Qingmaye resource, and a new variety of the multi-resistant Qingmaye type red-orange-core Chinese cabbage is bred on the basis of maintaining the typical characteristics such as Maye type cyan stem, green leaves, no burr and the like. Through the methoddisclosed by the invention, the bred new variety has short childbearing period, strong combining ability, Maye type cyan stem, deep green outer leaves, spherical leaves and red orange core, middle walnut texture, high resistance to various diseases such as viral disease, downy mildew, soft rot, tipburn and the like, bright color, high carotenoid content and obviously higher nutritional values than common cabbage, and can be cultivated as a specific vegetable, thereby obviously increasing the value of garden truck.

The invention discloses a mini-STR kit for a trace and degradable test material. The kit comprises specific amplification primers of 20 pairs of core loci, the core PCR products of the specific amplification primers are all less than 250bp; wherein the STR locus is shown in the specification; D16S539, D18S51, D21S11, D2S441, D13S317, FGA, D7S820, Amelogenin, D3S1358, D12S391, CSF1PO, D1S1656, THO1, D19S433, D8S1179, D10S1248, THOX, vWA, D5S818, and D2S1338 are used in the method. According to the invention, a mini amplification system with 20 gene loci is established, amplicon fragments are further shortened and are controlled within 250bp, and the detection number of the gene loci in a degradation detection material is increased; aiming at detection of difficult detection materials suchas trace detection materials and degradation detection materials, the method has the advantages of rapidness, high sensitivity and strong adaptability; the method is suitable for difficult samples which are highly degraded or have high inhibitor content, and can successfully obtain STR typing of special biological samples such as avicennia corpses, teeth, toenails, contact cast-off cells, old casesamples and the like in practical application.

The invention provides a clonal eosinophilia fusion gene detection probe composition, a kit and application thereof. According to the invention, firstly, related fusion genes are classified, wherein core genes FGFR1, PDGFRA and PDGFRB for clonal eosinophilia fusion detection are I-type genes, so that full-length transcripts of the genes are used as target regions; and other genes are II-type genes, so that only fragments near the fusion breakpoint are used as target regions. According to a base complementary pairing principle, complementary sequences of target regions are designed as probes to form a 'liquid phase chip', the probes relate to 39 target genes, eosinophilia fusion genes are detected by capturing corresponding target sequences in a transcriptome library and combining high-throughput sequencing, multiple fusion can be detected at a time, new fusion can be discovered, the detection cost is reduced, and the accuracy of a detection result is improved.

The invention relates to the field of the animal biomedical engineering and specifically discloses a preparation method of recombinant pichia pastoris for surface display of porcine epidemic diarrhea virus core antigen COE protein. A yeast alpha-agglutinin surface display system is adopted to express the antigen core gene, namely COE gene, of the PEDV, and the COE protein is anchored on the cell walls of the yeast, and therefore, the preparation method of recombinant pichia pastoris for surface display of porcine epidemic diarrhea virus core antigen COE protein is provided. The recombinant pichia pastoris can be prepared into novel vaccine products having excellent industrialization prospect; the popularization and application of the vaccines play a positive role in preventing viral diarrhea having serious harm to the pig industry and have great practical significance for protecting and promoting healthy development of the pig industry, and meanwhile, remarkable social and economic benefits can be achieved, and therefore, the vaccines have vast market and industrialization prospect.

The invention discloses the application of a small molecular compound in the preparation of silkworm antiviral drugs. The acquisition of the small molecular compound involved in the present invention is based on the mainstream molecular docking technology. For the core gene necessary for the silkworm baculovirus, its three-dimensional structure is obtained by using virtual modeling technology, and the small molecule database is screened by molecular docking. The obtained small molecule The compound is tested for activity, and finally the small molecule compound with effective effect in the present invention is obtained. When the final concentration of the small molecular compound is above 1 μg / mL, it can completely inhibit the replication of BmNPV, and can continue to inhibit the replication of BmNPV after the BmNPV completes the invasion of cells, and has a therapeutic effect. The invention can be used for the research, development and production of medicines for preventing and treating silkworm blood type pus disease, and has the value of application and popularization.