64 results about "Core gene" patented technology

The invention relates to the technical field of plant genetic engineering and genetic breeding, in particular to a method for regulating and controlling thermal morphogenesis of arabidopsis thaliana and application. The method is realized by regulating and controlling an ATG8 gene in arabidopsis thaliana, and the ATG8 gene is a combination of ATG8b and ATG8e, or a combination of ATG8f and ATG8h. The application comprises a thermal morphogenesis response weakening plant obtained through cultivation, or a seed thereof, a propagation material, and a kit for identifying the thermal morphogenesis ability of arabidopsis thaliana and weakening the thermal morphogenesis ability of arabidopsis thaliana. The invention discloses a new function of the ATG8 gene for the first time, and rigorous experiments prove that a plurality of members (ATG8b, ATG8e, ATG8f and ATG8h) of the ATG8 gene family positively regulate thermal morphological establishment of arabidopsis thaliana, so that people's understanding of the effect of autophagy in plant temperature sensing and adaptive growth is expanded. The function of the autophagy core gene ATG8 is combined with the breeding target of crop heat-resistant plant type improvement for the first time, and a brand new technical path is provided.

The invention provides the application of the OsLNHG1 gene in regulating and controlling the nitrogen utilization efficiency and the yield of the rice, in a low-nitrogen paddy field, the plant height of an OsLNHG1 gene overexpression plant is remarkably increased, and the tiller number is a core agronomic character for directly determining the yield of the rice. In a normal nitrogen paddy field, the plant height and the effective tiller number of an OsLNHG1 gene overexpressed plant are remarkably increased, a core gene resource is provided for cultivating a new variety of fertilizer-saving high-yield rice, and the gene has great significance for agricultural sustainable development.

This invention discloses a multi-scale prediction method and system for the maximum growth rate of soil microorganisms based on genomic features, involving the interdisciplinary fields of microbial ecology and genomics. The method includes: genomic data preparation, core genomic feature extraction, single-scale growth rate prediction, community-scale extrapolation, and result verification and output. The system includes a genomic data processing unit, a core feature extraction module, a growth rate prediction model, a community-scale extrapolation module, and a result visualization and verification unit. This invention overcomes the limitations of traditional culture methods and in-situ measurement methods, achieving high-throughput and accurate prediction of the growth rates of culturable and uncultured microorganisms, and solving the problems of low coverage, fragmented scales, and high costs of existing technologies. It is applicable to soil microbial function assessment, carbon cycle model parameterization, and ecosystem management.

The invention discloses a method for improving yield of branched chain aldehyde of lactococcus lactis based on regulation and control of a luxS gene and application, and belongs to the field of system biology. Gene function verification and genome scale metabolic network model analysis are creatively combined, the phenotypic effect of the luxS gene is confirmed, and more importantly, the internal action mechanism of the luxS gene is disclosed from the system level, that is, the whole metabolic network flow is influenced by regulating and controlling multiple key enzymes such as transaminase, decarboxylase, dehydrogenase and decarboxylase. The invention reveals that the quorum sensing core gene luxS has a new function of regulating and controlling synthesis of branched chain aldehyde in lactococcus lactis for the first time, and an intercellular communication system is directly linked with production of specific flavor metabolites. The invention provides a brand-new and higher-level regulation target and thought for producing flavor substances by microbial fermentation, and gets rid of the limitation that only metabolic pathway terminal enzyme is singly modified traditionally.

One bacterial strain, designated HC1M1C21T, was isolated from human faeces and analyzed using phylogenetic, morphologic and biochemical approaches. Phylogenetic analyses based on 16S rRNA sequences and a set of 92 bacterial core genes indicated that this strain belongs to the Lachnospiraceae family and clusters near Pilosibacter fragilis (96.9% 16S rRNA sequence identity). HC1M1C21T has a DNA G+C content of 48.7 mol%. This strain is anaerobic, Gram- stain-positive, non-motile and non-spore-forming. HC1M1C21T cells appear as single rods or chained rods with tapered ends. Optimal growth was observed at 37°C, at pH between 5.5 and 6.5 and at salinity below 10 g / L. HC1M1C21T is a potent butyrate producer. The invention pertains to a new bacterial strain that produces butyrate and several independent factors with anti-inflammatory properties.

The invention discloses a primer group, a kit and a detection system for thalassemia gene detection. In order to more comprehensively and accurately detect point mutation, small insertion, small deletion, gene fusion mutation, large fragment deletion and gene structure variation on a thalassemia core gene and a modified gene in single detection, the invention provides a thalassemia gene detection primer group and a kit. Based on third-generation sequencing platforms such as a single-base nanopore sequencing platform and the like, all point mutation, small insertion, small deletion and gene fusion mutation in the amplification range of the thalassemia core gene and the modified gene can be detected by utilizing the primer group or the kit; and 60 alpha large fragment deletion mutations, 60 beta large fragment deletion mutations and 7 gene structure variations. The method is beneficial to rapid and comprehensive screening of thalassemia, and is high in detection sensitivity and good in accuracy.

The invention discloses sinorhizium xinjiangense APXJHT24-1 which has the functions of promoting growth and inhibiting aspergillus flavus, and the preservation number of the sinorhizium xinjiangense APXJHT24-1 is CCTCC (China Center for Type Culture Collection) M 2026229. According to the invention, the integration of two major functions of'generation of a characteristic antibacterial volatile matter of dimethyl disulfide 'and'efficient symbiotic nitrogen fixation' on a single strain is realized in Sinkiang Sinkiang rhizobium for the first time. The dimethyl disulfide released by the strain can accurately down-regulate expression of toxin synthesis key genes and spore development core genes in the aspergillus flavus, so that efficient bacteriostasis is realized on the molecular level. Through one-time inoculation, the strain can synchronously achieve the dual purposes of growth promotion and nitrogen fixation and source bacteriostasis at the rhizosphere ecological niche of the peanuts. Especially aiming at the biological characteristics of overground flowering and underground fruiting of peanuts, a lasting biological fumigation protection layer can be formed in a soil microenvironment in a legume development critical period.

The application discloses a molecular typing method of Staphylococcus capitis, and first establishes an operation technology for molecular typing of Staphylococcus capitis by using a multi-locus sequence typing system (MLST). Seven conservative gene fragments are confirmed by screening, all known Staphylococcus capitis genomes are typed based on the combined sequences, ST types and CC types are defined, and a system evolution tree based on core genome SNP differences is compared, so that reliable typing results can be obtained. Seven pairs of PCR primers are designed and verified, and the Staphylococcus capitis can be typed by MLST. The seven conservative gene combinations and amplification primers used in the typing technology are used for the first time, and the PCR reaction system and conditions are optimized by the inventors. The technical process can be used for molecular classification and epidemiological monitoring of Staphylococcus capitis.

This invention discloses a PhlD gene based on translation rate optimization and its applications, belonging to the fields of genetic engineering and protein engineering. Without altering the amino acid sequence of the PhlD protein, this invention optimizes the translation rate of the original PhlD sequence through synonymous codon substitution, enabling rapid translation within the coding domains. Translation deceleration sites are introduced into the contiguous regions of the coding sequence domains, matching the ribosomal translation rate with the protein co-translational folding process, significantly improving the soluble expression level of the PhlD protein. The PhlD gene with optimized translation rate results in a 5.5-fold increase in the soluble expression level of the encoded PhlD protein compared to the original sequence. Engineered bacteria containing this optimized gene show a 4-fold increase in the yield of phloroglucinol during fermentation compared to the control. This provides a core gene resource for the efficient soluble expression of the PhlD protein and the industrial production of phloroglucinol.

The application discloses a detection primer and probe for detecting a pathogenic bacterium of a crop stem base rot and a chip digital PCR detection method. TEF-1 alpha The application takes a pathogenic bacterium of a crop stem base rot as a target, and the pathogenic bacterium is a Fusarium pseudograminearum Tri Core gene cluster nucleic acid fragments are respectively designed to obtain high-specificity detection primer pairs and probes, and a double-chip digital PCR quantitative detection method for a dominant pathogenic bacterium of a wheat stem base rot is established according to the same. The correlation coefficients of ideal copy numbers and actual copy numbers of target samples detected by the double-chip digital PCR quantitative method are all greater than 0.9998, the target DNA can be more accurately detected, the double-chip digital PCR quantitative method shows higher accuracy and sensitivity than fluorescence quantitative PCR in a complex rhizosphere soil sample, and the double-chip digital PCR quantitative method has application prospects in accurate quantification and detection of nucleic acids of the dominant pathogenic bacterium of the wheat stem base rot.

The invention relates to the technical field of microbiological detection, in particular to a detection method based on specificity of PCR (Polymerase Chain Reaction) and CRISPR-Cas12a (Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR-Cas12a) for bifidobacterium and application. The method comprises the following steps: amplifying a common single copy core gene of bifidobacterium by adopting a polymerase chain reaction (PCR) technology to obtain an amplification product; and an amplification product is detected by adopting a clustered regular interval short palindromic repeat related protein (CRISPR-Cas12a) system. The detection method is simple and convenient to operate, only comprises two parts of PCR amplification reaction and Crispr reaction, and the evaluation method of the detection result is simple, short in overall time consumption, high in efficiency, capable of detecting various bifidobacteria and definitely free of specificity to eight non-bifidobacteria, and wide in application range.

The invention relates to the technical field of disease prognosis and molecular biology, in particular to application of immune-related polygenes in prognosis evaluation of diffuse large B-cell lymphoma. The biomarkers are DES, CHIT1, FGF14, CCR4, CTLA4 and VGF (Vertical Growth Factor). According to the method, core genes related to immunity are screened on the basis of diffuse large B cell lymphoma transcriptome data such as GEO and TCGA, and a multi-gene immune model: RS = (-0.0306) * DES + (-0.4683) * CHIT1 + (1.1418) * FGF14 + (0.1132) * CCR4 + (-0.07) * CTLA4 + (-0.3463) * VGF is constructed; according to the immune polygene immune model, diffuse large B-cell lymphoma patients can be divided into high and low RS groups, the survival prognosis of high-RS people is proved to be obviously lower than that of low-RS people, and the survival prognosis can be used as an independent prognosis index.

An ornithine decarboxylase gene related to Auricularia auricula-judae degradation and application thereof belong to the field of bioengineering technology. In order to solve the technical problem that there is lack of key functional genes and molecular targets which can be used for early identification, anti-degradation molecular breeding and strain rejuvenation due to continuous subculture leading to degradation in Auricularia auricula-judae industry, based on the G1, G10 and G20 mycelium transcriptome difference analysis of Auricularia auricula-judae strain Heiwei S1908, the ornithine decarboxylase gene g4162 significantly differentially expressed between normal mycelium and G20 degraded mycelium is screened, the molecular function of the gene in the degradation of Auricularia auricula-judae subculture is determined, and the system biology information prediction of the gene coding protein is completed, which provides core gene resources and theoretical basis for the degradation mechanism research, molecular detection kit development, anti-degradation germplasm screening and gene breeding of Auricularia auricula-judae.

The application discloses a method for constructing a gene regulation network based on meta-analysis, and aims to solve the problem that a gene regulation network has a large error and a low accuracy because different research data are not completely homogeneous when the gene regulation network is expanded by combining data of multiple studies to enlarge a sample size, and the method comprises the following steps: performing meta-analysis on each transcriptomic gene expression dataset obtained to obtain a significant differential gene list; obtaining a transcription factor gene list according to a species to which the transcriptomic gene expression data belong, and generating a core gene list according to the transcription factor gene list and the significant differential gene; calculating a Pearson correlation coefficient of each core gene and each significant differential gene, and constructing a co-expression network according to the Pearson correlation coefficient; clustering the co-expression network to obtain a plurality of differential gene modules with high correlation of the core genes; and combining biological knowledge and a structural equation model to construct a corresponding gene regulation network according to each module. The application belongs to the field of gene regulation networks.

The present invention relates to a microbiological typing method comprising providing: a database of genetic profiles of microbial strains; and a phylogenetic tree, assigning the genetic spectrum to a location in the phylogenetic tree, and assigning a frequency of variation of the spectrum in the tree. The microbial typing comprises the following steps: measuring genetic spectrums of the microorganisms, determining the variation probability of each genetic spectrum in a database relative to the measured genetic spectrums, and calculating the variation probability according to the variation frequency; and determining that the microorganism belongs to a classification unit of the tree if at least the probability of the classification unit is above a threshold. According to the invention, the phylogenetic tree is constructed from the core genome of the microbial species and a predefined set of genetic markers is selected from the auxiliary genome of the microbial species.

The invention discloses sinorhizobium meliloti ASZJHZ25-4 with functions of efficiently promoting growth and inhibiting aspergillus flavus and application of the sinorhizobium meliloti ASZJHZ25-4, and the preservation number of the sinorhizobium meliloti ASZJHZ25-4 is CCTCC M 2026230. According to the invention, the integration of two functions of generating a characteristic antibacterial volatile matter of 2-methylbutyric acid and efficiently symbiotic nitrogen fixation on a single strain is realized in the sinorhizobium meliloti for the first time. The 2-methylbutyric acid volatile matter released by the strain can accurately down-regulate expression of toxin synthesis key genes and spore development core genes in aspergillus flavus, so that efficient bacteriostasis is realized on the molecular level. Through one-time inoculation, the dual purposes of growth promotion and nitrogen fixation and source bacteriostasis can be synchronously achieved at the rhizosphere ecological niche of the peanuts. Especially aiming at the biological characteristics of overground flowering and underground fruiting of peanuts, a lasting biological fumigation protection layer is formed in a soil microenvironment in a legume development critical period.

The invention discloses a chlamydia psittaci cgMLST molecular typing method, and belongs to the field of microbial molecular typing and tracing. According to the invention, an operation technology for carrying out molecular typing on chlamydia psittaci by using a core genome multi-site sequence typing system (cgMLST) is established for the first time. The chlamydia psittaci cgMLST system is established through the processes of collecting chlamydia psittaci genomes in a disclosed genome database, obtaining core genomes through quality control screening and generic genome analysis, screening core genes suitable for typing under multiple screening conditions and the like. And comparing with a phylogenetic analysis result based on core genome SNP difference to confirm the resolution and reliability of the typing system. According to the typing technology, the core genome of the chlamydia psittaci is determined for the first time, and the typing technology can be used for molecular typing and epidemiological monitoring of the chlamydia psittaci.

The invention discloses a sinorhizobium fredii strain capable of promoting growth and inhibiting aspergillus flavus and application of the sinorhizobium fredii strain. The sinorhizobium fredii strain is classified and named as sinorhizobium fredii, and the preservation number of the sinorhizobium fredii strain is CCTCC (China Center for Type Culture Collection) M 20253054. According to the invention, the dual functions of generating a specific bacteriostatic volatile matter 2-nonanol and efficiently symbiotic nitrogen fixation are integrated in the sinorhizobium freudenreanum for the first time. 2-nonanol released by the strain can accurately down-regulate expression of toxin synthesis key genes and spore development core genes in aspergillus flavus, so that efficient bacteriostasis is realized on the molecular level. Through one-time inoculation, the strain can synchronously achieve the dual purposes of growth promotion and nitrogen fixation and source bacteriostasis at the rhizosphere ecological niche of the peanuts. Especially aiming at the biological characteristics of overground flowering and underground fruiting of peanuts, a lasting biological fumigation protection layer can be formed in a soil microenvironment in a legume development critical period.

A traditional Chinese medicine intelligent screening method based on network targeting comprehensive indexes belongs to the technical field of traditional Chinese medicine intelligent screening. The method comprises the following steps: constructing a target set A and a protein interaction network database corresponding to each traditional Chinese medicine; constructing a disease core gene set B based on a protein interaction network; calculating a network targeting comprehensive index based on the target point set A and the disease core gene set B; calculating the network targeting comprehensive index of each traditional Chinese medicine and then performing ascending sorting, wherein the smaller the network targeting comprehensive index is, the stronger the network targeting relevance between the traditional Chinese medicine and the disease is; and matching the traditional Chinese medicines according to the obtained ascending order, and generating a structured and explainable final recommendation report. According to the method, the disease-related gene identification accuracy is improved, the biological interpretation of the result is enhanced, the standardized processing of integrating multi-source data into intelligent recommendation is realized, and the urgent demand of efficient and accurate screening in modern research of traditional Chinese medicines is met.

The invention provides application of an OsTNR2 gene in regulation and control of nitrogen utilization efficiency and yield of rice, a nucleotide sequence of the OsTNR2 gene is shown as SEQ ID NO.1, and the OsTNR2 gene negatively regulates and controls the nitrogen utilization efficiency and yield of the rice under a low-nitrogen condition. Under a low-nitrogen (0.2 mM NH4NO3) culture condition, compared with a wild type (WT), the overground part height and biomass of the Ostnr2 mutant after the OsTNR2 gene is knocked out are remarkably increased, and the inhibition of low-nitrogen stress on plant growth is effectively overcome. Under a low-nitrogen culture condition, the content ratio of 15N in the overground part to the underground part of the Ostnr2 mutant is increased, which proves that the deletion of the OsTNR2 gene enhances the nitrogen absorption or transport capacity of the plant under the low-nitrogen condition, thereby relieving the inhibition effect of low-nitrogen stress on plant growth to a certain extent. The cooperative regulation capability of the OsTNR2 gene provides a core gene resource for cultivating a new variety of fertilizer-saving high-yield rice, and has great significance for agricultural sustainable development.

This invention discloses novel specific molecular targets for *Bacteroides fragilis* and its BFS17, along with a rapid detection method. The molecular target nucleotide sequence of *Bacteroides fragilis* is shown in SEQ ID NO.1, and the molecular target nucleotide sequence of *Bacteroides fragilis* BFS17 is shown in SEQ ID NO.2. This invention uses a pan-genomic approach to obtain the specific core genes of *Bacteroides fragilis* and the probiotic strain *Bacteroides fragilis* BFS17, and designs characteristic primers that can sensitively detect *Bacteroides fragilis* and the probiotic strain *Bacteroides fragilis* BFS17. Furthermore, this method can rapidly and easily distinguish *Bacteroides fragilis*, the probiotic strain *Bacteroides fragilis* BFS17, and other microbial strains. This invention has the advantages of being simple and rapid to operate, highly specific, quantifiable, and inexpensive.

This invention relates to the field of genetic engineering technology, and provides a ZmMTⅠ‑3 The application of genes in reducing cadmium accumulation in maize, the ZmMTⅠ‑3 The CDS sequence of the gene is shown in SEQ ID NO.1. This invention also provides a method for reducing cadmium accumulation in maize and its application in cultivating low-cadmium-accumulation maize plants. Furthermore, this invention provides a low-cadmium-accumulation maize plant obtained using the above method. This invention clarifies for the first time... ZmMTⅠ‑3 The gene plays a role in regulating cadmium accumulation in maize, and knocking out this gene can significantly reduce the absorption and accumulation of cadmium in maize plants. This provides a novel gene target and molecular breeding approach for the cultivation of low-cadmium-accumulation maize materials, effectively making up for the lack of existing core gene resources for low-cadmium regulation in maize. It is suitable for the safe production of maize in cadmium-contaminated farmland and has important application value.

The invention belongs to the fields of biotechnology, protein engineering and plant protection science, and particularly relates to an antagonistic protein mutant for cultivating a sweet potato composite virus disease (SPVD) resistant plant and application of the antagonistic protein mutant. The key pathogenic factor of the SPVD is the RNase3 protein coded by the sweet potato chlorotic stunt virus (SPCSV), and the RNase3 protein is used as an RNA silence suppressor (RSS) to destroy host immunity. The invention provides an SPCSV-RNase3 antagonistic protein mutant, which is derived from a natural antagonistic protein, namely a sweet potato trypsin inhibitor (IbSPLTI-a). According to the present invention, a compound structure model (such as construction through AlphaFold2) of wild type IbSPLTI-a and RNase3 is analyzed, and the IbSPLTI-a is modified by using a protein directed evolution strategy, particularly by using a hotspot amino acid scanning and protein surface design strategy, such that the high-activity inhibition mutant (such as IbSPLTI-a-m0805) is successfully created and screened; in-vivo and in-vitro function verification shows that the binding affinity of the mutant (such as IbSPLTI-a-m0805) and SPCSV-RNase 3 is remarkably enhanced, and the mutant shows a virus accumulation inhibition effect superior to that of a wild type IbSPLTI-a. The invention also provides a nucleic acid sequence for coding the mutant, a plant expression vector containing the sequence, and a method for culturing an anti-SPVD transgenic plant (especially sweet potato) by using the mutant. The invention provides a core gene resource and a technical path for genetic improvement of SPVD and development of a novel antiviral protein preparation.