105 results about "Gene regulatory network" patented technology

The invention relates to the technical field of biomedicine, and particularly relates to a method for constructing a genetic regulation network based on total-transcriptome high-throughput sequencing.The method comprises the steps that a disease related competitive endogenous RNA regulation network is constructed on the basis of total-transcriptome high-throughput sequencing data through the screening of differently expressed genes, lncRNA, circRNA and miRNA, the co-expression analysis of the genes, the lnc RNA and the circRNA and the miRNA combining target prediction, key genes in the regulation network are screened according to a random walking algorithm, the pathway in which the key genes are significantly enriched is determined through pathway enrichment analysis, and the biological mechanism of the key genes in the disease regulation process is analyzed combining with the genetic regulation relationship of the pathway. The invention provides methods for the verification of the disease related key genes and the construction of the regulation network of the key genes, and a novel approach is provided for the study of generating developing mechanisms of complicated diseases.

The present invention relates to novel miRNA molecules, more particularly to novel miRNA molecules isolated from human embryonic stem cells. The miRNA molecules provided by the present invention can be usefully used as a molecular marker for early developmental stages of undifferentiated human embryonic stem cells. Also, the miRNA molecules of the present invention may play an important role in the regulation of mammalian embryonic stem cells. Therefore, the miRNA molecules can be usefully used for analyzing regulatory networks of human embryonic stem cells.

The invention relates to a gene regulation and control network constructing method based on a Bayesian network. Relationship of all gene expressions in a species or a tissue is wholly analyzed and studied in simulation by constructing a gene regulation and control network model. The construction of the gene regulation and control network model comprises the following steps: A. identifying the best node order by a binary particle swarm optimization algorithm with memories, wherein after the particle speed is updated by the binary particle swarm optimization algorithm, the speed of part particles is varied and a searching space is searched, and skipping from local is taken as optimal; and B. inputting the obtained best node order as a K2 algorithm, then executing K2 algorithm and studying the structure of the Bayesian network. The method has the advantages of fast convergence, simple calculation, easy realization and the like, provides clues for studying regulation and control of gene transcription level, shows the structure of the gene regulation and control network more accurately and has important theoretical significance and practical value in many fields, such as biology, medicine science, pharmacy and the like.

The invention discloses an inference method of a stepwise regression gene regulatory network. The method comprises the following steps of: A, reading a gene expression data matrix and a gene perturbation data matrix; B, confirming whether the gene expression data matrix and the gene perturbation data matrix are standardized data or not; C, respectively carrying out data normalization on the gene expression data matrix and the gene perturbation data matrix to form the standardized data; D, analyzing the standardized data and calculating all inter-gene correlation coefficient matrixes; and E, visualizing the inter-gene correlation coefficient matrixes into a network to obtain a gene regulatory network chart. The method can select optimal regression subsets to solve the problem of high-dimension small-sample experimental data, gradually select the most influential regulator for a target gene, accord with the true condition of the gene regulatory network, and be superior to similar methods in calculation precision and calculation efficiency along with the enlargement of the gene regulatory network scale and the network sparsity.

The invention belongs to the technical field of data processing and discloses a method for constructing a double-layer gene regulation and control network. The method comprises following steps: analyzing expression data of an important regulation and control factor miRNA in a network; selecting miRNA relevant to multiple types of cancer by means of the statistics examination method; measuring Pearson correlation among the miRNA; ordering the miRNA according to the correlation and the number of controllable cancer, screening out strongly correlated miRNA and obtaining target genes thereof and interacting relations among the target genes. The present invention discloses the mechanism of gene and miRNA involved in the regulation of biological processes such as cancer and helps to understand the relationship between genes and cancer and other complex diseases and provides reference for the development of biopharmaceuticals and targeted therapy for pan-cancer. Identifying cancer-associated miRNAs and genes can be used to elucidate the mechanisms of cooperation that are involved in the regulation of key factors that influence a variety of cancer processes, cancer risk prediction, and the development of bio-targeted drugs.

Gene regulation network is reconstructed using time series microarray data under the method of the Bayesian network. Particular power-law function is used to calculate the joint probabilities among genes across time points. This invention discloses the use of the downhill simplex algorithm to find global maxima of interrelational likelihood. Arcs with higher frequencies are selected to establish the gene regulation network. Prior knowledge may be included into candidate gene networks to accelerate search for best networks.

The invention discloses a riemerella anatipestifer CH1 global regulator phoP attenuated strain and a construction method thereof, and belongs to the technical field of biological engineering. The attenuated strain is constructed by knocking out a phoP gene by means of a homologous recombination method and utilizing a bacterial conjugation method. The prepared attenuated strain has a preservation number of CCTCC NO:M2014557, and is preserved on November 7, 2014. The attenuated strain has low toxicity, and a foundation is established for construction of subsequent vaccine vectors and construction of a riemerella anatipestifer gene regulatory network. The invention further provides application of the attenuated strain in vaccines.

The invention relates to a conjoined grafting method for plant meristem. The conjoined grafting method for the plant meristem is characterized in that the conjoined grafting method through meristem between different phylum, klasse, order, family, genus and species of plantae is provided, and the conjoined grafting method comprises an apcial meristem, a lateral meristem and an intercalary meristem. The meristem possesses massive reversible gene regulatory networks and spatial and temporal expression opportunities due to the fact that the meristem has high cell division ability and is a key tissue of organogenesis and morphogenesis and a starting point of organ and tissue differentiation. Therefore, conducting moderate manual injury intervention and conjoined grafting on the meristem can greatly increase opportunity of successful grafting, and enlarges boundary capable of being conducted grafting between different phylum, klasse, order, family, genus and species of the plantae. Due to the fact that scion and rootstock whole plant are not separated, bud mutation and fructification are promoted to obtain grafting progeny seeds, exchanging and changing of part genetic materials of grafting progeny can be achieved, and consequently, low coat and safe material exchanging of extra distant species of the plantae can be achieved, variation is induced, and then a new germplasm of a scion plant is created.

The invention described herein provides compositions and reagents for assembling a tripartite complex at a specific location of a target DNA. The invention also provides methods for using the complex to, for example, label a specific genomic locus, to regulate the expression of a target gene, or to create a gene regulatory network.

The invention provides a gene regulation and control network reconstruction method based on gene expression data, and relates to the technical field of gene regulation and control network reconstruction in bioinformatics. The method comprises the following steps that: obtaining gene expression quantity data required for reconstruction; carrying out normalization processing on the data; carrying out predictive modeling on a target gene expression quantity; predicting the target gene expression quantity; analyzing a regulation and control relationship between an input feature gene and a target gene; and reconstructing the gene regulation and control network. By use of the gene regulation and control network reconstruction method based on the gene expression data, high-accuracy gene regulation and control network modeling is realized according to gene expression data, and an Elman neural network optimized by a differential evolution algorithm predicts the gene expression quantity so as tohave the advantages of high operation speed and high accuracy. In addition, simulation data can be used for solving the problem of an insufficient data quantity is solved, the gene regulation and control network which is finally established exhibits good accuracy, has a wide applicable range, can be suitable for different pieces of gene expression data and exhibits good transportability.

The invention relates to a gene regulation and control network re-building method based on cross-platform gene expression data. Gene expression data from p sequencing platforms is obtained and preprocessed according to the characteristics of the cross-platform gene expression data, n gene expression quantities are extracted from each gene sample, a father and son node sets of each gene expression quantity is obtained based on the mixed type condition independence testing of partial correlation coefficients, the father and son node sets are applied to the three processes of a learning network framework of cross-platform cause-effect network structure learning, v-structure determination and maximized direction marking, and a cross-platform gene regulation and control network is rebuilt. The problems of a cross-platform gene regulation and control network are solved through a cause-effect graph model, high-dimensional gene regulation and control network rebuilding can be directly and effectively carried out through the cross-platform gene expression data, data excess smoothness and other problems caused in the data preprocessing process are avoided, and the correctness rate and the recall rate of the rebuilding result of the cross-platform gene regulation and control network are increased.

Embodiments of methods are provided that can be used to estimate network relationships between genes of an organism using time course expression data and a set of linear differential equations. Aikaike's Information Criterion and mask tools can be used to reduce the number of elements in a matrix by determining which elements are zero or not significantly changed under the conditions of the study. Maximum likelihood estimation and new statistical methods are used to evaluate the significance of a proposed network relationship.

The invention discloses an establishment method of a gene regulating network based on two-way XGBoost. Aiming at the characteristics of time sequence gene expression data, the relationship between anexpression value of a t moment gene and the positions before and after other gene t moment points is analyzed, and local-in and local-out two-way models are established; in a single model, establishment of the gene regulating network is divided into multiple regression equations, the condition of high-order time lag is fully considered, weights of characteristics in a single regression equation are evaluated through the XGBoost, and finally a rank of regulating relationships is acquired; normalization residuals are adopted for carrying out secondary weighting on the characteristics in each independent regression equation, and the weights of the two-way models for the same pair of regulating relationships are combined to form a value to serve as the final score of each pair of gene regulating relationships. The method is suitable for establishing different scales of gene regulating networks under the time sequence gene expression data, and the accuracy of network deduction can be effectively improved.

By a computer search for upstream promoter elements (DSE, PSE) typical of small nuclear RNA (snRNA) genes, we have identified a number of previously unrecognized, putative transcription units whose predicted products are novel noncoding RNAs with homology to protein-coding genes. By elucidating the function of one of them, we provide evidence for the existence of a sense/antisense-based gene regulation network where part of the Pol III transcriptome could control its Pol II counterpart.

The invention relates to the technical field of swarm intelligence, in particular to a swarm robot dynamic encirclement control method and system based on a gene regulation and control network, and the method comprises the steps: controlling swarm robots to search in an unknown environment until a target is searched; after the swarm robot searches the target, detecting the environment informationaround the target by using a sensor carried by the swarm robot to obtain the relative position of the target and the relative position of an obstacle in the environment around the target; then, inputting the relative position of the target and the relative position of the obstacle into the gene regulation and control network model for iterative training to obtain a trained gene regulation and control network model; finally, generating a swarm aggregation form according to the trained gene regulation and control network model, controlling the swarm robot to surround the target according to theswarm aggregation form. The method can efficiently complete the encirclement task in real time in a complex, dynamic and unknown environment.

The invention provides a method for constructing a gene regulatory network based on structural prediction. The method includes the following steps: first calculating a coefficient matrix, determiningthe correlation between genes, by calculating the Peason coefficient, the mutual information, and the maximum mutual information between the genes, as a basis for screening potential parent node sets;then, performing structural prediction, using an obtained coefficient matrix between genes as the basis for determining the potential parent node sets of the genes, and selecting a potential parent node set for each gene; finally, performing the structural learning and parameter learning of the gene regulatory network. The method predicts the potential parent node sets of the genes by a method based on of the combination of the Person coefficient, the mutual information, and the maximum mutual information, narrows the search range of structure learning, shortens the construction time of the gene regulatory network to a certain extent, improves computing performance, can build a large-scale gene regulatory network quickly and accurately, and further understands the gene regulatory mechanism of organisms.

The invention discloses a swarm robot aggregation control method based on a three-layer gene regulatory network, which comprises a creation layer, a formation layer and a control layer based on a gene regulatory network model, importing the relative distance from each robot to the target and the relative distance between every two robots, which are acquired by a swarm robot airborne sensor, into a creation layer to obtain a local coordinate system at the current moment; calling the coordinate information of all individuals in the local coordinate system at the previous moment to perform coordinate conversion, and acquiring target coordinate information, obstacle coordinate information and coordinate information of each robot in the local coordinate system at the current moment; the target coordinate information and the obstacle coordinate information are imported into a formation layer, a surrounding form is obtained, and a surrounding control point of each robot is obtained; and the coordinate information and the surrounding control point of each robot are imported into a control layer, and the group robots are guided to move towards the target for surrounding. According to the invention, the self-adaptive hunting of the target in the three-dimensional space with the failure of the global positioning system is realized.

The invention relates to a biochip gene expression spectrum system, especially a method of constructing gene regulation and control network by continuous gene expression spectrum. It considers the size, direction and time phase difference of gene regulation and control. By large-scale gene chip continuous expression spectrum data, it considers the change condition of expression quantity with the time, calculates the distance of regulation and control relationship between genes, makes clustering analysis on the distance to construct a large-scale gene regulation and control network. The steps: a), obtaining several continuous expression spectrum gene chip data at time points of several genes; b), determining the distance of regulation and control relation between all the genes; c) selecting specific distances to construct gene regulation and control network.