Process of constructing gene regulation network by continuous gene expression spectrum

A gene regulation network and gene expression profiling technology, applied in the field of biochip gene expression profiling systems, can solve the problems of limited technology, inability to accurately measure gene expression levels, and inability to guarantee accuracy.

Inactive Publication Date: 2005-01-05
INST OF COMPUTING TECH CHINESE ACAD OF SCI
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Problems solved by technology

[0007] Second, the current technology for large-scale measurement of gene expression levels is limited, and it is impossible to accurately measure the expression levels of all genes within a certain time range
Although the development of gene chip technology can measure the expression levels of thousands of genes at the same time, there is no guarantee that the measured expression levels of these thousands of genes have high accuracy, and the gene regulatory network can be constructed from these data , the accuracy will not be very high

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  • Process of constructing gene regulation network by continuous gene expression spectrum
  • Process of constructing gene regulation network by continuous gene expression spectrum
  • Process of constructing gene regulation network by continuous gene expression spectrum

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Embodiment Construction

[0028] In order to achieve the above goals, the following four steps are required to construct a gene regulatory network from a continuous gene expression profile (see figure 1 ):

[0029] 1. Obtain continuous expression profile chip data

[0030] Taking M genes as an example, the gene expression profile data of the M genes at N times are determined by using a gene chip.

[0031] For a specific biological tissue (or cell), the method of sampling at specific time intervals is used to measure the expression profile data of M genes at N times by using gene chip technology. For example: the expression levels of 2,000 genes related to human liver at 0 minutes, 5 minutes, 10 minutes, 15 minutes, ..., 270 minutes, 275 minutes of cell culture, a total of 56 time points are a set of continuous genes Microarray expression data. The continuous gene chip expression profile data can be expressed as: a table composed of M rows and N columns, each row represents a gene, and each column re...

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Abstract

The invention relates to a biochip gene expression spectrum system, especially a method of constructing gene regulation and control network by continuous gene expression spectrum. It considers the size, direction and time phase difference of gene regulation and control. By large-scale gene chip continuous expression spectrum data, it considers the change condition of expression quantity with the time, calculates the distance of regulation and control relationship between genes, makes clustering analysis on the distance to construct a large-scale gene regulation and control network. The steps: a), obtaining several continuous expression spectrum gene chip data at time points of several genes; b), determining the distance of regulation and control relation between all the genes; c) selecting specific distances to construct gene regulation and control network.

Description

technical field [0001] The invention relates to a biochip gene expression spectrum system and the technical field of analysis of large-scale gene chip continuous expression spectrum data. In particular, a method for constructing gene regulatory networks from continuous gene expression profiles. Background technique [0002] Gene expression profiling chips can be divided into differential expression chips and continuous expression chips. Differential expression chip can detect the gene expression level of normal tissue and tumor tissue at the same time, and find abnormally expressed genes. The continuous expression chip can detect the change of gene expression in tissue over a period of time, and discover the rule of gene expression change over time. The detection chip for detecting gene variation and abnormal gene expression has been widely used in clinical practice, while the data analysis of continuous expression chip is mostly used in the field of scientific research. ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 蔡伦李志广熊江辉
Owner INST OF COMPUTING TECH CHINESE ACAD OF SCI
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