2595 results about "Gene expression" patented technology

Provided herein are methods of detecting an analyte of interest to interrogate spatial gene expression in a sample using RNA-templated ligation.

The present invention belongs to the technical field of immunology, and in particular, relates to a humanized nanoantibody against TNF-α and its application. The present invention provides a humanized nanoantibody against TNF-α, and a recombinant humanized nanoantibody against TNF-α prepared by expressing the gene of the humanized nanoantibody against TNF-α. The humanized nanoantibody against TNF-α prepared by the present invention can specifically recognize the TNF-α antigen, thereby accurately targeting the inflammatory site, reducing nonspecific reactions, and improving the therapeutic effect.

The invention belongs to the technical field of immunology, and particularly relates to an anti-TNF-alpha nano antibody and application thereof. The invention provides an anti-TNF (Tumor Necrosis Factor)-alpha nano antibody. The anti-TNF-alpha recombinant nano antibody is prepared by performing gene expression on the anti-TNF-alpha nano antibody. The anti-TNF-alpha nano antibody prepared by the invention can specifically recognize a TNF-alpha antigen so as to accurately target an inflammation part, reduce non-specific reaction and improve the treatment effect.

The invention discloses bacillus velezensis as well as fermentation product filtrate and a biological product thereof, and belongs to the technical field of microorganisms, a bacillus velezensis BTN-HB-F4 strain is separated and purified from prinsepia utilis royle in Harba snow mountain in Yunnan province, the fermentation product filtrate of the bacillus velezensis has excellent HAS inhibition capacity and remarkable DPPH and ABTS free radical scavenging capacity, and the biological product of the bacillus velezensis has the advantages that the biological product can be used for preparing biological products of the prinsepia utilis royle; a good anti-oxidation effect can be realized; secondly, under various concentrations, fermentation product filtrate can remarkably reduce the proportion of SA-beta-Gal positive cells, gene expression of matrix metalloproteinase 3 can be remarkably inhibited, and a good anti-aging effect is achieved; in addition, fermentation product filtrate can remarkably inhibit generation of zebra fish melanin and has a good whitening effect; the secretion amount of cell inflammatory factors IL-6 and TNF-alpha can be remarkably inhibited, and a good relieving effect is achieved; finally, the fermentation product filtrate can effectively inhibit the growth of pathogenic bacteria staphylococcus aureus, and has the potential of regulating the skin micro-ecology.

The invention discloses recombinant escherichia coli for inactivating or weakly expressing SPMB family inner membrane protein and application of the recombinant escherichia coli in production of L-amino acid. The invention relates to the technical field of gene engineering, and provides recombinant escherichia coli, and SPMB family inner membrane protein in the recombinant escherichia coli is inactivated or reduced in activity. According to the invention, the yjiG gene (coding gene of SPMB family inner membrane protein) in escherichia coli is knocked out, so that the expression intensity of the yjiG gene is weakened, and fermentation production strains of L-threonine, L-tryptophan, L-arginine and L-valine, which are more cost-effective, can be produced. The invention has important significance for improving the fermentation yield of bacteria, namely L-threonine, L-tryptophan, L-arginine and L-valine.

The invention discloses a hot air type qPCR instrument, a multi-channel detection optical system and a detection method, and relates to the field of molecular biological detection. The problems that an optical detection system adopted by an existing qPCR instrument mostly depends on a single-channel or double-channel design, sample hole sites need to be switched one by one and detected in a time-sharing mode in the detection process, namely, the efficiency is low in the face of a high-throughput requirement scene are solved, and the system combines multiple LED light sources with an optical filter to generate excitation wavelengths to form a light source module; after the multi-stage dichroscope is utilized to form a light combination module, the light combination module is transmitted to a reaction hole through one branch of the forked optical fiber to excite fluorescent substances, a fluorescent signal is collected through a common end of the forked optical fiber, the other branch of the forked optical fiber outputs a fluorescent signal, and the fluorescent signal is split by the reflection grating to form a light splitting module. And the array CMOS is combined with a spectrum unmixing algorithm to form a detection module for synchronous receiving, so that multi-channel simultaneous high-sensitivity detection is realized. The method is also suitable for the field of multi-pathogen detection and gene expression analysis.

The invention belongs to the technical field of biological feature recognition, and relates to a Transform-based gene data mining method, which comprises the following steps of: loading gene expression data from a file, and preprocessing; inputting the data into a designed model for training and evaluation; the designed model comprises a CNN (Convolutional Neural Network) and an improved Transform model; the improved Transform model comprises an encoder and a classifier; the encoder adopts a multi-head self-attention mechanism, a convolutional self-attention mechanism and a feedforward neural network; processing the data by using the trained model, and storing the fusion attention weight obtained by the coding layer in the data processing process; and finally, averaging the fusion attention weights of all batches, calculating the comprehensive attention weight of each gene at different time points, and identifying the most important gene by calculating and analyzing the attention score of each gene. According to the method, the problems of scarcity and imbalance of gene data can be solved, the risk of overfitting is reduced, and the precision of gene data analysis is improved.

Aspects of the disclosure relate to complexes comprising a muscle-targeting agent covalently linked to a molecular payload. In some embodiments, the muscle-targeting agent specifically binds to an internalizing cell surface receptor on muscle cells. In some embodiments, the molecular payload promotes the expression or activity of a functional dystrophin protein. In some embodiments, the molecular payload is an oligonucleotide, such as an antisense oligonucleotide, e.g., an oligonucleotide that causes exon skipping in a mRNA expressed from a mutant DMD allele.

The invention belongs to the field of agricultural chemistry and bio-fertilizer, and discloses a fertilizer synergist based on polyaspartic acid, alginate oligosaccharide and mannan oligosaccharide and a preparation method of the fertilizer synergist. According to the synergist, polyaspartic acid derived from plant source amino acid, alginate oligosaccharide extracted from natural brown algae and mannan oligosaccharide extracted from plants are used as main raw materials, and the polyaspartic acid, the alginate oligosaccharide and the mannan oligosaccharide cooperate to form an induction-complexation-adsorption three-effect-in-one mechanism, wherein the alginate oligosaccharide and the mannan oligosaccharide induce plant root activity and stress resistance gene expression; the polyaspartic acid is dominated to form a multi-point weak complex network, so that slow release and controlled release of nitrogen and potassium ions are realized; the three components cooperate on the surface of soil colloid to form an adsorption layer, and nutrient leaching loss is reduced. The preparation process adopts a green process of'enzymatic pretreatment-segmented complexing-low-temperature forming ', so that the oligosaccharide activity is maintained, and the use of an organic solvent is avoided. The obtained synergist can significantly improve the nitrogen utilization rate and the root activity and prolong the fertilizer efficiency period, and is suitable for green and efficient agricultural production of various crops.

Aspects of the disclosure relate to complexes comprising a muscle-targeting agent covalently linked to a molecular payload. In some embodiments, the muscle-targeting agent specifically binds to an internalizing cell surface receptor on muscle cells. In some embodiments, the molecular payload promotes the expression or activity of a functional dystrophin protein. In some embodiments, the molecular payload is an oligonucleotide, such as an antisense oligonucleotide, e.g., an oligonucleotide that causes exon skipping in a mRNA expressed from a mutant DMD allele.

Aspects of the disclosure relate to complexes comprising a muscle-targeting agent covalently linked to a molecular payload. In some embodiments, the muscle-targeting agent specifically binds to an internalizing cell surface receptor on muscle cells. In some embodiments, the molecular payload promotes the expression or activity of a functional dystrophin protein. In some embodiments, the molecular payload is an oligonucleotide, such as an antisense oligonucleotide, e.g., an oligonucleotide that causes exon skipping in a mRNA expressed from a mutant DMD allele.

The invention discloses a method for traceless gene editing of gram negative bacteria, a carrier and application of the carrier, and belongs to the technical field of microbial genetic engineering. The invention provides a vector for performing traceless gene editing on bordetella pertussis. The vector simultaneously comprises an antibiotic positive screening gene expression box and a sacB and galk negative screening gene expression box. According to the method, the sacB gene and the galk genome are synthesized into the double-reverse-screening system for the first time, and compared with a single-reverse-screening system, the reverse-screening efficiency is remarkably improved, and the dosage of a reverse-screening reagent is remarkably reduced. The invention provides a novel gene editing method for non-mode bacteria such as pertussis bordetella and the like, and has a good application prospect.

The invention discloses application of an MCU (microprogrammed control unit) inhibitor in preparation of a medicine for preventing or treating intestinal ischemia-reperfusion injury, and relates to the technical field of biological medicine, and the MCU inhibitor comprises one or two of a reagent for inhibiting MCU gene expression and a reagent for inhibiting MCU protein activity. The MCU inhibitor disclosed by the invention is beneficial to relieving intestinal mucosa injury and inflammatory change of intestinal tissues, improving completeness of a villus structure, reducing pathological injury degree score of the intestinal tissues and relieving cell apoptosis degree in the intestinal tissues, so that the MCU inhibitor is beneficial to relieving programmed death of intestinal mucosa cells; as a main component, the pharmaceutical composition is beneficial for preventing or treating intestinal ischemia-reperfusion injury.

The invention provides an scRNA-seq data clustering method, system and device based on ZINB distribution and graph attention. The scRNA-seq data clustering system mainly comprises three core modules: a ZINB auto-encoder, which is used for modeling scRNA-seq data based on zero-expansion negative binomial distribution, generating robust potential representation through a denoising auto-encoder, and accurately capturing sparsity, excessive discreteness and shedding events of gene expression; the residual image attention auto-encoder is used for constructing a cell relation graph by using a Pearson correlation coefficient, dynamically learning a neighborhood weight in combination with a multi-head attention mechanism, retaining original features through residual connection, and relieving the excessive smoothness problem of image convolution; and a deep clustering model is self-optimized: target distribution and soft label distribution are minimized through KL divergence, and end-to-end joint optimization of embedded learning and clustering is realized.

The present disclosure is directed to controlling pest infestation by inhibiting one or more biological functions in an invertebrate pest. The disclosure discloses methods and compositions for use in controlling pest infestation by feeding one or more different recombinant double stranded RNA molecules to the pest in order to achieve a reduction in pest infestation through suppression of gene expression. The disclosure also discloses methods and compositions for targeted genome editing in the pest in order to achieve a reduction in pest infestation through disruption of protein activity. The disclosure is also directed to methods for making transgenic plants that express the double stranded RNA molecules and targeted genome editing constructs for use in protecting plants from pest infestation.

An RNA inhibitor for inhibiting gene expression of a complement system, or a pharmaceutically acceptable salt thereof. The structure of the RNA inhibitor further contains vector structures 5'MVIP and 3'MVIP. The provided RNA inhibitor interferes with the translation template function of the mRNA of the complement system, and continuously and efficiently inhibits the gene expression of the complement system, so that CFB, C5 and C3 protein levels of the complement system in blood are continuously reduced. The RNA inhibitor can be used for treating and / or preventing diseases associated with an increased level of the complement system.

The invention provides a structure, a preparation method and an application method of a group of anti-wrinkle compact cyclopeptide compounds. The anti-wrinkle compact cyclic peptide compound comprises an anti-wrinkle cyclic peptide-1 with a sequence of c (CDLARLAMYA), and / or an anti-wrinkle cyclic peptide-2 with a sequence of c (KTFKKTFK). The promotion rates of the anti-wrinkle cyclopeptide-1 to zebra fish collagen gene expression col1a1a, col1a1b and col1a2 under the concentration of 0.001% are respectively 156%, 87% and 103%. The promotion rate of the cyclopeptide on zebra fish elastin Elna gene expression under the test concentration of 0.001% is 132%. Under the concentration of 0.001%, the promotion rates of the anti-wrinkle cyclopeptide-2 on zebra fish collagen gene expression col1a1a, col1a1b and col1a2 are 94%, 84% and 66% respectively. The promotion rate of the cyclopeptide on zebra fish elastin Elna gene expression under the test concentration of 0.001% is 66%.

The present disclosure provides, in part, double stranded compounds, including oligonucleotides such as siRNA, that comprise one or more independent occurrences of a modified nucleotide components of Formula (I), Formula (II), Formula (III), or Formula (IV) as described herein, as well as their methods of use in inhibiting gene expression and the treatment of various disorders.

The present invention relates to a nucleic acid molecule inhibiting factor 11 (FXI) gene expression in a cell by means of RNAi, comprising a sense sequence and an antisense sequence complementary to each other, or consisting of a sense sequence and an antisense sequence complementary to each other. The FXI inhibition rate of the nucleic acid molecule is significantly superior to that of other small nucleic acid molecules which inhibit the expression of FXI by means of RNAi.

The invention discloses a novel application of an RNF6 gene, namely, an application of screening a medicine for preventing and / or treating Zika virus infection, aiming at inhibiting expression of the RNF6 gene or knocking out the RNF6 gene. A series of functional experiments prove that the RNF6 gene promotes the replication of the Zika virus, and the purpose of reducing the replication of the Zika virus can be achieved by using an expression inhibitor of the RNF6 gene or knocking out the RNF6 gene; experimental results show that the RNF6 gene can be used as a potential target for preventing and / or treating Zika virus infection, and a new direction is provided for development of drugs for preventing and / or treating Zika virus infection targeting the RNF6 gene in the future.

The present disclosure relates to RNAi agents, e.g., double stranded RNAi agents such as siRNAs, able to Inhibin Subunit Beta E (INHBE) gene expression. Also disclosed are pharmaceutical compositions that include INHBE RNAi agents and methods of use thereof. The INHBE RNAi agents disclosed herein may be conjugated to targeting ligands to facilitate the delivery to cells, including to hepatocytes. Delivery of the INHBE RNAi agents in vivo provides for inhibition of INHBE gene expression. The RNAi agents can be used in methods of treatment of diseases, disorders, or symptoms mediated in part by INHBE gene expression, such as obesity, diabetes, liver inflammation, dyslipidemia, or metabolic disease.

The invention provides a risk early warning method and system for stomach cancer postoperative malnutrition, and relates to the technical field of medical treatment, and the method comprises the steps: obtaining gene expression data and clinical indexes of a stomach cancer patient based on a cancer genome map database; gastric cancer methylation data is collected, and a methylation risk scoring model is constructed to determine a methylation risk score; performing quantitative evaluation on the nutritional status of the gastric cancer patient based on a nutritional risk screening tool to obtain a nutritional risk level; determining risk characteristics related to stomach cancer postoperative dystrophy according to the gene expression data, the clinical indexes, the methylation risk score and the nutrition risk grade, and establishing a risk early warning model based on a support vector machine; and acquiring current risk data of the patient side, inputting the current risk data into the risk early warning model, outputting a risk score and a risk level, generating a risk intervention measure according to the risk level, and feeding back the risk intervention measure to the medical care side, so that accurate and comprehensive early warning can be performed on the risk of postoperative malnutrition of the gastric cancer patient, and a timely and effective nutrition intervention scheme is formulated.

The invention relates to the technical field of medical information, in particular to a construction method and system of a liver cancer gene database and a medium, and the method comprises the following steps: constructing a spatial neighborhood relationship based on cell space coordinates, and defining mutually communicated cell populations as spatial neighborhood hyperedges; labeling functional modules according to the gene expression values, and associating cell nodes in the same functional module as functional module hyperedges; constructing a space-time dynamic hypergraph structure based on the spatial neighborhood hyperedge and the functional module hyperedge; and carrying out local aggregation on node features of the space-time dynamic hypergraph structure to obtain a gene expression correlation weight matrix between a target node and a domain node so as to construct a liver cancer gene database. According to the method, a high-quality data support and analysis platform is provided for liver cancer mechanism research by comprehensively describing a spatial interaction network of liver cancer cells, dynamic evolution of functional modules and gene expression correlation.

Methods and systems disclosed herein can improve inference of gene expression using cell-free DNA fragments. In an aspect, the present disclosure provides a computer-implemented method for inferring gene expression, the method comprising: obtaining a biological sample from a subject; extracting cell-free deoxyribonucleic acid (cfDNA) from the biological sample, wherein the cfDNA comprises a plurality of cfDNA fragments; performing a sequencing assay on the plurality of cfDNA fragments to generate a plurality of cfDNA sequencing fragments; computer processing the plurality of cfDNA sequencing fragments; and calculating, based at least in part on the computer processing, a gene expression score for a gene in a plurality of genes, wherein the gene expression score indicates a probability of expression or non-expression of the gene in the plurality of genes.

The invention belongs to the technical field of genetic engineering and agricultural biology, and provides application of dsRNA for specifically inhibiting RpHsp22.6 gene expression in preparation of insecticides, and the nucleotide sequence of the dsRNA is shown as SEQ ID NO.12. The invention further provides a preparation method of the dsRNA. The invention also provides a method for relieving soybean blue syndrome and provides a small heat shock protein RpHsp22.6 of Emblycoris lividipennis. According to the invention, the problem of how to more effectively and more environmentally prevent and treat the plant diseases and insect pests of the Adelphocoris indica is solved, and the effect of reducing the pathogenicity of the Adelphocoris indica to the soybeans is achieved.

The invention relates to a siRNA for targeted regulation of PCSK9 gene expression and a use thereof. Experimental results show that some oligonucleotide sequences subject to alternate modification and specific template modification can significantly inhibit PCSK9 expression, and can be used for developing a drug for treating PCSK9-related diseases.

A dsRNA and double-stranded RNAi agent for inhibiting the expression of INHBE and a use thereof. The dsRNA and the double-stranded RNAi agent can be used for inhibiting the expression of INHBE to prevent and treat a metabolic disorder (such as a metabolic syndrome).

The invention relates to the technical field of bioinformatics, in particular to a biological information analysis system based on a large model technology, which comprises a data analysis calibration module, a problem disassembly module, an analysis task arrangement module, a result mapping module and a feedback iteration module. According to the method, genome comparison and clinical phenotype timestamps are dynamically calibrated, time sequence dislocation deviation is eliminated, base complementary pairing is combined with protein network anomaly screening, low-abundance collaborative variation capture is enhanced, genotype-phenotype discrete distribution quantifies and unifies multi-modal data benchmark, and the problem of multi-source heterogeneous standardization deficiency is solved; the method comprises the following steps: classifying and integrating pathogenic gene semantic weights by structural variation, balancing a statistical threshold and a biological function, dynamically optimizing an analysis sequence, synchronously covering a key mutation region, improving function annotation of a non-coding region, integrating gene expression clustering and protein network topology in a three-dimensional distribution manner, breaking through two-dimensional space limitation, performing closed-loop feedback to correct a threshold iteration elimination rule, and finally obtaining a high-quality gene expression cluster. And the genetic heterogeneity false positive rate is reduced.

The invention belongs to the field of daily chemicals, and provides a composition with a longevity gene activation effect, a product containing the composition and application of the composition, and the composition provided by the invention comprises hexapeptide, chlamydomonas and cornflower extract. The hexapeptide, the chlamydomonas nivalis and the cornflower extract are compounded for use, so that the expression of longevity genes KLOTHO and SIRT1 can be remarkably improved. Once the longevity gene serving as the upstream of a signal regulation pathway is activated, various downstream signal pathways can be regulated, such as inhibition of expression of inflammation-related genes, promotion of DNA repair, telomere homeostasis, gene expression of mitochondrial energy and the like, promotion of synthesis of collagen and the like, and finally, longevity of cells and improvement of skin phenotypes are promoted. The effects of resisting wrinkles, tightening and the like are achieved. Therefore, the composition provided by the invention can activate long-life gene expression and regulate and control multiple signal pathways, has potential effects of improving light aging, tightening and resisting wrinkles and the like, and is an anti-aging composition with great potential.

The invention relates to the technical field of microorganisms, and particularly discloses a recombinant microorganism and application thereof in fermentation production of decarboxylated carnosine. Compared with an original strain, the recombinant microorganism disclosed by the invention has the advantages that the expression of the mdtK gene is enhanced; the starting strain is escherichia coli capable of producing decarboxylated carnosine. When the recombinant microorganism is used for fermentation production, the yield of the decarboxylated carnosine can be increased, L-histidine, histamine or beta-alanine and other precursors do not need to be added in the production process, and an effective novel method is provided for industrial production of the decarboxylated carnosine.