3622 results about "Gene expression" patented technology

Provided herein are methods of detecting an analyte of interest to interrogate spatial gene expression in a sample using RNA-templated ligation.

The present invention belongs to the technical field of immunology, and in particular, relates to a humanized nanoantibody against TNF-α and its application. The present invention provides a humanized nanoantibody against TNF-α, and a recombinant humanized nanoantibody against TNF-α prepared by expressing the gene of the humanized nanoantibody against TNF-α. The humanized nanoantibody against TNF-α prepared by the present invention can specifically recognize the TNF-α antigen, thereby accurately targeting the inflammatory site, reducing nonspecific reactions, and improving the therapeutic effect.

The invention belongs to the technical field of immunology, and particularly relates to an anti-TNF-alpha nano antibody and application thereof. The invention provides an anti-TNF (Tumor Necrosis Factor)-alpha nano antibody. The anti-TNF-alpha recombinant nano antibody is prepared by performing gene expression on the anti-TNF-alpha nano antibody. The anti-TNF-alpha nano antibody prepared by the invention can specifically recognize a TNF-alpha antigen so as to accurately target an inflammation part, reduce non-specific reaction and improve the treatment effect.

The invention discloses bacillus velezensis as well as fermentation product filtrate and a biological product thereof, and belongs to the technical field of microorganisms, a bacillus velezensis BTN-HB-F4 strain is separated and purified from prinsepia utilis royle in Harba snow mountain in Yunnan province, the fermentation product filtrate of the bacillus velezensis has excellent HAS inhibition capacity and remarkable DPPH and ABTS free radical scavenging capacity, and the biological product of the bacillus velezensis has the advantages that the biological product can be used for preparing biological products of the prinsepia utilis royle; a good anti-oxidation effect can be realized; secondly, under various concentrations, fermentation product filtrate can remarkably reduce the proportion of SA-beta-Gal positive cells, gene expression of matrix metalloproteinase 3 can be remarkably inhibited, and a good anti-aging effect is achieved; in addition, fermentation product filtrate can remarkably inhibit generation of zebra fish melanin and has a good whitening effect; the secretion amount of cell inflammatory factors IL-6 and TNF-alpha can be remarkably inhibited, and a good relieving effect is achieved; finally, the fermentation product filtrate can effectively inhibit the growth of pathogenic bacteria staphylococcus aureus, and has the potential of regulating the skin micro-ecology.

The invention discloses a streptomycete recombinant engineering strain for producing mycosporine-like metabolites, a gene expression cassette is integrated in a genome of the streptomycete recombinant engineering strain, the gene expression cassette is recombined to an attB site of a chromosome in a streptomycete chassis strain, the gene expression cassette is an operon controlled by a single promoter, and the operon is an operon controlled by a single promoter. The operon comprises 2-4 biosynthetic gene elements in MysA, MysB, MysC and MysD or mysDp334 in a synthetic pathway of mycosporine-like metabolites. The invention also discloses a construction method of the streptomyces recombinant engineering strain and an application of the streptomyces recombinant engineering strain in preparation of the mycosporine-like metabolites. A mycosporine-like metabolite is efficiently produced by heterologous expression of a mycosporine-like metabolite synthesis gene element in streptomyces, and four mycosporine-like metabolite products with high yield and strong ultraviolet absorption activity can be obtained by reconstruction of the biosynthesis gene element and recombinant expression in streptomyces pluvialis.

Provided are a salt tolerance related protein GmXTH32 having an amino acid sequence as shown in sequence 2, and a related biomaterial and use thereof. The use comprises: improving salt tolerance of plants and / or preparing products for improving salt tolerance of plants; and improving the survival rate of plants under salt stress and / or preparing products for improving the survival rate of plants under salt stress. The salt tolerance of plants can be changed by regulating and controlling the expression level of a coding gene of the protein in the plants, and the salt tolerance of the plants can be improved by reducing the expression level of the coding gene of the protein.

The invention discloses recombinant escherichia coli for inactivating or weakly expressing SPMB family inner membrane protein and application of the recombinant escherichia coli in production of L-amino acid. The invention relates to the technical field of gene engineering, and provides recombinant escherichia coli, and SPMB family inner membrane protein in the recombinant escherichia coli is inactivated or reduced in activity. According to the invention, the yjiG gene (coding gene of SPMB family inner membrane protein) in escherichia coli is knocked out, so that the expression intensity of the yjiG gene is weakened, and fermentation production strains of L-threonine, L-tryptophan, L-arginine and L-valine, which are more cost-effective, can be produced. The invention has important significance for improving the fermentation yield of bacteria, namely L-threonine, L-tryptophan, L-arginine and L-valine.

The invention discloses a hot air type qPCR instrument, a multi-channel detection optical system and a detection method, and relates to the field of molecular biological detection. The problems that an optical detection system adopted by an existing qPCR instrument mostly depends on a single-channel or double-channel design, sample hole sites need to be switched one by one and detected in a time-sharing mode in the detection process, namely, the efficiency is low in the face of a high-throughput requirement scene are solved, and the system combines multiple LED light sources with an optical filter to generate excitation wavelengths to form a light source module; after the multi-stage dichroscope is utilized to form a light combination module, the light combination module is transmitted to a reaction hole through one branch of the forked optical fiber to excite fluorescent substances, a fluorescent signal is collected through a common end of the forked optical fiber, the other branch of the forked optical fiber outputs a fluorescent signal, and the fluorescent signal is split by the reflection grating to form a light splitting module. And the array CMOS is combined with a spectrum unmixing algorithm to form a detection module for synchronous receiving, so that multi-channel simultaneous high-sensitivity detection is realized. The method is also suitable for the field of multi-pathogen detection and gene expression analysis.

The invention provides a high-stability recombinant humanized elastin, a genetically engineered bacterium for expressing the protein and application of the high-stability recombinant humanized elastin, and belongs to the technical field of binding tissue peptides. The nucleotide sequence of the recombinant humanized elastin is shown as SEQ ID NO.1; and the amino acid sequence is as shown in SEQ ID NO.4. According to the invention, the nucleotide sequence of the humanized elastin is subjected to codon optimization and transferred into escherichia coli to construct the genetically engineered bacterium for expressing the recombinant humanized elastin, so that the expression quantity of the recombinant humanized elastin can be increased, the stability of the recombinant humanized elastin can be improved, and the recombinant humanized elastin can be used for preparing the recombinant humanized elastin. The migration rate of the recombinant humanized elastin to the HSF cell is improved, and the expression quantity of the h-ELN gene, the h-COL1A1 gene and the h-COL3A1 gene of the HSF cell is improved.

The present application relates to an RNA inhibitor or a pharmaceutically acceptable salt thereof for inhibiting the expression of an LPA gene comprising a complementary region of an antisense strand, the complementary region comprises at least 15 consecutive nucleotides, wherein the antisense strand comprises the following nucleotide sequence: any sequence of SEQ ID NO. 414-418, a sequence having at least 15 consecutive nucleotides identical to that in SEQ ID NO. 414-418, or a sequence having no more than three different nucleotides from the above sequences. The RNA inhibitor of the present application directly degrades LPA mRNA, continuously and efficiently inhibits LPA gene expression, and can be used to treat or prevent cardio-cerebrovascular diseases mediated by LPA genes.

The invention belongs to the technical field of biological feature recognition, and relates to a Transform-based gene data mining method, which comprises the following steps of: loading gene expression data from a file, and preprocessing; inputting the data into a designed model for training and evaluation; the designed model comprises a CNN (Convolutional Neural Network) and an improved Transform model; the improved Transform model comprises an encoder and a classifier; the encoder adopts a multi-head self-attention mechanism, a convolutional self-attention mechanism and a feedforward neural network; processing the data by using the trained model, and storing the fusion attention weight obtained by the coding layer in the data processing process; and finally, averaging the fusion attention weights of all batches, calculating the comprehensive attention weight of each gene at different time points, and identifying the most important gene by calculating and analyzing the attention score of each gene. According to the method, the problems of scarcity and imbalance of gene data can be solved, the risk of overfitting is reduced, and the precision of gene data analysis is improved.

The invention belongs to the technical field of fungal gene engineering, and discloses a shiitake mushroom ABC transporter gene LeABC2 and application thereof in regulation and control of shiitake mushroom heat resistance. Whole genome association analysis is carried out based on heat resistance phenotype data of 133 shiitake mushroom strains, a candidate gene LeABC2 for regulating heat resistance is obtained, a LeABC2 gene RNA interference vector is constructed, the gene function is researched through an agrobacterium tumefaciens-mediated genetic transformation method, and the result shows that the LeABC2 gene negatively regulates the heat resistance of shiitake mushroom hyphae, the heat resistance of the hyphae can be improved by interfering the expression of the gene, and the gene has a good application prospect. A new solution thought is provided for breaking through the problem that the yield and quality are influenced by high temperature in the mushroom industry.

The invention belongs to the technical field of plant genetic engineering, and discloses an application of a corn gene ZmGG1 in control of corn yield, a protein encoded by the ZmGG1 is shown in SEQ ID NO.2, and the gene is located in the ninth chromosome of corn and used for controlling the row number and the ear weight of corn ears. The gene is knocked out by using a CRISPR / Cas9 technology, gene expression is inhibited, and the ear row number and the ear weight of corn can be increased.

The disclosure features novel lipids and compositions involving the same. Lipid nanoparticles (e.g., empty LNPs or loaded LNPs) include a novel cationic lipid as well as additional lipids such as ionizable lipids, phospholipids, structural lipids, and PEG lipids. Lipid nanoparticles (e.g., empty LNPs or loaded LNPs) further including therapeutic and / or prophylactic agents such as RNA are useful in the delivery of therapeutic and / or prophylactic agents to mammalian cells or organs to, for example, regulate polypeptide, protein, or gene expression.

Aspects of the disclosure relate to complexes comprising a muscle-targeting agent covalently linked to a molecular payload. In some embodiments, the muscle-targeting agent specifically binds to an internalizing cell surface receptor on muscle cells. In some embodiments, the molecular payload promotes the expression or activity of a functional dystrophin protein. In some embodiments, the molecular payload is an oligonucleotide, such as an antisense oligonucleotide, e.g., an oligonucleotide that causes exon skipping in a mRNA expressed from a mutant DMD allele.

The invention belongs to the field of biological medicines, and particularly relates to a modified double-stranded oligonucleotide, and a conjugate and a composition thereof. The double-stranded oligonucleotide provided by the invention effectively enhances the activity of the double-stranded oligonucleotide for inhibiting the expression of the target gene by performing specific modification on the nucleotide at a specific site, can efficiently inhibit the expression of the target gene in both in-vitro and in-vivo experiments, and surprisingly, the double-stranded oligonucleotide provided by the invention has a good application prospect. The modification on the double-stranded oligonucleotide can be generally used for siRNA designed for various different target genes, and the inhibition rate of the siRNA on the target genes can be effectively improved.

The invention belongs to the field of agricultural chemistry and bio-fertilizer, and discloses a fertilizer synergist based on polyaspartic acid, alginate oligosaccharide and mannan oligosaccharide and a preparation method of the fertilizer synergist. According to the synergist, polyaspartic acid derived from plant source amino acid, alginate oligosaccharide extracted from natural brown algae and mannan oligosaccharide extracted from plants are used as main raw materials, and the polyaspartic acid, the alginate oligosaccharide and the mannan oligosaccharide cooperate to form an induction-complexation-adsorption three-effect-in-one mechanism, wherein the alginate oligosaccharide and the mannan oligosaccharide induce plant root activity and stress resistance gene expression; the polyaspartic acid is dominated to form a multi-point weak complex network, so that slow release and controlled release of nitrogen and potassium ions are realized; the three components cooperate on the surface of soil colloid to form an adsorption layer, and nutrient leaching loss is reduced. The preparation process adopts a green process of'enzymatic pretreatment-segmented complexing-low-temperature forming ', so that the oligosaccharide activity is maintained, and the use of an organic solvent is avoided. The obtained synergist can significantly improve the nitrogen utilization rate and the root activity and prolong the fertilizer efficiency period, and is suitable for green and efficient agricultural production of various crops.

Aspects of the disclosure relate to complexes comprising a muscle-targeting agent covalently linked to a molecular payload. In some embodiments, the muscle-targeting agent specifically binds to an internalizing cell surface receptor on muscle cells. In some embodiments, the molecular payload promotes the expression or activity of a functional dystrophin protein. In some embodiments, the molecular payload is an oligonucleotide, such as an antisense oligonucleotide, e.g., an oligonucleotide that causes exon skipping in a mRNA expressed from a mutant DMD allele.

Aspects of the disclosure relate to complexes comprising a muscle-targeting agent covalently linked to a molecular payload. In some embodiments, the muscle-targeting agent specifically binds to an internalizing cell surface receptor on muscle cells. In some embodiments, the molecular payload promotes the expression or activity of a functional dystrophin protein. In some embodiments, the molecular payload is an oligonucleotide, such as an antisense oligonucleotide, e.g., an oligonucleotide that causes exon skipping in a mRNA expressed from a mutant DMD allele.

The invention discloses a method for traceless gene editing of gram negative bacteria, a carrier and application of the carrier, and belongs to the technical field of microbial genetic engineering. The invention provides a vector for performing traceless gene editing on bordetella pertussis. The vector simultaneously comprises an antibiotic positive screening gene expression box and a sacB and galk negative screening gene expression box. According to the method, the sacB gene and the galk genome are synthesized into the double-reverse-screening system for the first time, and compared with a single-reverse-screening system, the reverse-screening efficiency is remarkably improved, and the dosage of a reverse-screening reagent is remarkably reduced. The invention provides a novel gene editing method for non-mode bacteria such as pertussis bordetella and the like, and has a good application prospect.

The invention discloses an OpenCRISPR (Open Clustered Regularly Interspaced Short Palindromic Repeats)-NLS (Non-Least Squares) fusion protein for gene editing of penaeus monodon, a construction method of the OpenCRISPR-NLS fusion protein and a nucleotide construct containing the OpenCRISPR The invention also discloses an application of the fusion protein or the nucleotide construct in gene editing of penaeus monodon and an application of the fusion protein or the nucleotide construct in population improvement of aquaculture penaeus monodon. According to the invention, codon optimization is carried out on a coding sequence of OpenCRISPR-1 protein, and a nuclear localization signal (NLS) is fused with the OpenCRISPR protein subjected to codon optimization; the method has the advantages of being efficient in gene expression, accurate in targeting positioning capacity, excellent in genome editing efficiency, capable of reducing the off-target effect, wide in adaptability, capable of promoting sustainable aquaculture, remarkable in economic and social value and the like.

The invention discloses application of an MCU (microprogrammed control unit) inhibitor in preparation of a medicine for preventing or treating intestinal ischemia-reperfusion injury, and relates to the technical field of biological medicine, and the MCU inhibitor comprises one or two of a reagent for inhibiting MCU gene expression and a reagent for inhibiting MCU protein activity. The MCU inhibitor disclosed by the invention is beneficial to relieving intestinal mucosa injury and inflammatory change of intestinal tissues, improving completeness of a villus structure, reducing pathological injury degree score of the intestinal tissues and relieving cell apoptosis degree in the intestinal tissues, so that the MCU inhibitor is beneficial to relieving programmed death of intestinal mucosa cells; as a main component, the pharmaceutical composition is beneficial for preventing or treating intestinal ischemia-reperfusion injury.

The invention provides an scRNA-seq data clustering method, system and device based on ZINB distribution and graph attention. The scRNA-seq data clustering system mainly comprises three core modules: a ZINB auto-encoder, which is used for modeling scRNA-seq data based on zero-expansion negative binomial distribution, generating robust potential representation through a denoising auto-encoder, and accurately capturing sparsity, excessive discreteness and shedding events of gene expression; the residual image attention auto-encoder is used for constructing a cell relation graph by using a Pearson correlation coefficient, dynamically learning a neighborhood weight in combination with a multi-head attention mechanism, retaining original features through residual connection, and relieving the excessive smoothness problem of image convolution; and a deep clustering model is self-optimized: target distribution and soft label distribution are minimized through KL divergence, and end-to-end joint optimization of embedded learning and clustering is realized.

The present disclosure is directed to controlling pest infestation by inhibiting one or more biological functions in an invertebrate pest. The disclosure discloses methods and compositions for use in controlling pest infestation by feeding one or more different recombinant double stranded RNA molecules to the pest in order to achieve a reduction in pest infestation through suppression of gene expression. The disclosure also discloses methods and compositions for targeted genome editing in the pest in order to achieve a reduction in pest infestation through disruption of protein activity. The disclosure is also directed to methods for making transgenic plants that express the double stranded RNA molecules and targeted genome editing constructs for use in protecting plants from pest infestation.

The present disclosure belongs to the technical fields of computational medicine and clinical oncology, and specifically relates to a molecular subtyping method for gastric cancer (GC) based on metabolic genes, a construction method for a prognostic prediction model and applications. In the present disclosure, metabolic process alterations are overall assessed, and new metabolic transcription patterns based on 456 metabolic gene-related signatures are identified. Three different metabolic patterns are validated by unsupervised consensus clustering analysis and independent datasets, and molecular and clinical pathological features of these three metabolite clusters have different metabolic gene expressions, pathway enrichments, gene variations, and survival outcomes. In addition, in the present disclosure, a scoring scheme is constructed to assess metabolic patterns of individual tumors, revealing the relationships between the metabolic patterns with prognosis, cuproptosis and immune regulation, and having a good value for practical application.

An RNA inhibitor for inhibiting gene expression of a complement system, or a pharmaceutically acceptable salt thereof. The structure of the RNA inhibitor further contains vector structures 5'MVIP and 3'MVIP. The provided RNA inhibitor interferes with the translation template function of the mRNA of the complement system, and continuously and efficiently inhibits the gene expression of the complement system, so that CFB, C5 and C3 protein levels of the complement system in blood are continuously reduced. The RNA inhibitor can be used for treating and / or preventing diseases associated with an increased level of the complement system.

A dual screen technique using DEL and 2DCS screens with libraries of synthesized small molecules and synthesized RNA structures (4,096 targets) delivers bona fide ligand-RNA 3D fold target pairs. One of the newly discovered ligands bound a 5′GAG / 3′CCC internal loop that is present in pri-miR-27a, the oncogenic precursor of microRNA-27a. The DEL-derived pri-miR-27a ligand is cell-active, potently and selectively inhibits pri-miR-27a processing to reprogram gene expression and halt an otherwise invasive phenotype in triple-negative breast cancer cells.

The invention provides a structure, a preparation method and an application method of a group of anti-wrinkle compact cyclopeptide compounds. The anti-wrinkle compact cyclic peptide compound comprises an anti-wrinkle cyclic peptide-1 with a sequence of c (CDLARLAMYA), and / or an anti-wrinkle cyclic peptide-2 with a sequence of c (KTFKKTFK). The promotion rates of the anti-wrinkle cyclopeptide-1 to zebra fish collagen gene expression col1a1a, col1a1b and col1a2 under the concentration of 0.001% are respectively 156%, 87% and 103%. The promotion rate of the cyclopeptide on zebra fish elastin Elna gene expression under the test concentration of 0.001% is 132%. Under the concentration of 0.001%, the promotion rates of the anti-wrinkle cyclopeptide-2 on zebra fish collagen gene expression col1a1a, col1a1b and col1a2 are 94%, 84% and 66% respectively. The promotion rate of the cyclopeptide on zebra fish elastin Elna gene expression under the test concentration of 0.001% is 66%.

The invention discloses an auxiliary diagnosis method and system for bronchial asthma, and relates to the technical field of molecular diagnosis prediction.The auxiliary diagnosis method comprises the steps that an experimental group and a control group are defined, and RNA of the experimental group and RNA of the control group are extracted for low-temperature preservation; carrying out quantitative PCR (Polymerase Chain Reaction) analysis on the RNA stored at low temperature and extracting target cDNA; collecting clinical data of the experimental group and calculating the corresponding biomarker concentration according to the target cDNA; and constructing a bronchial asthma prediction model, outputting a predicted risk probability value of each patient suffering from asthma, and dynamically classifying and adjusting the treatment schemes of the patients. RNA samples of an experimental group and a control group are accurately extracted and stored, remarkably changed gene expression is obtained in combination with quantitative PCR analysis, the gene expression is further combined with biomarker concentration, dynamic monitoring of the asthma-related inflammatory response is successfully achieved, and the bronchial asthma risk of a patient can be accurately evaluated by constructing a bronchial asthma prediction model. And a personalized treatment scheme is provided according to comprehensive analysis of gene expression and biomarkers.

The present disclosure provides, in part, double stranded compounds, including oligonucleotides such as siRNA, that comprise one or more independent occurrences of a modified nucleotide components of Formula (I), Formula (II), Formula (III), or Formula (IV) as described herein, as well as their methods of use in inhibiting gene expression and the treatment of various disorders.