151 results about "DNA - Deoxyribonucleic acid" patented technology

Methods and systems disclosed herein can improve inference of gene expression using cell-free DNA fragments. In an aspect, the present disclosure provides a computer-implemented method for inferring gene expression, the method comprising: obtaining a biological sample from a subject; extracting cell-free deoxyribonucleic acid (cfDNA) from the biological sample, wherein the cfDNA comprises a plurality of cfDNA fragments; performing a sequencing assay on the plurality of cfDNA fragments to generate a plurality of cfDNA sequencing fragments; computer processing the plurality of cfDNA sequencing fragments; and calculating, based at least in part on the computer processing, a gene expression score for a gene in a plurality of genes, wherein the gene expression score indicates a probability of expression or non-expression of the gene in the plurality of genes.

The invention discloses a novel PDRN, a supramolecular liposome containing the PDRN as well as a preparation method and application of the supramolecular liposome. The preparation method of the novel PDRN comprises the following steps: (1) mixing a tissue raw material and PBS, crushing, carrying out enzymolysis, adding SDS, a Tween20 solution, polyethylene glycol octylphenol ether, a TE buffer solution and NaCl, stirring, centrifuging, mixing a supernatant with absolute ethyl alcohol, and standing to obtain a DNA crude extract; (2) carrying out breaking shearing treatment by using an ultrasonic breaking instrument; and (3) carrying out enzymolysis treatment by using restriction endonuclease and deoxyribonuclease, and the like. The PDRN supramolecular liposome is obtained by wrapping the PDRN with fatty acid extracted from marine microalgae. The fish testis are used as the raw material source, the selection range of the raw materials is widened, the preparation cost is reduced, and the organic reagent used in the preparation method is ethyl alcohol which is easy to remove and harmless.

Reported herein is, inter alia, a method for producing a trivalent bispecific antibody, the method comprising the steps of culturing a mammalian cell comprising a deoxyribonucleic acid encoding the trivalent bispecific antibody, and recovering the trivalent bispecific antibody from the cell or culture medium, wherein the deoxyribonucleic acid encoding the trivalent bispecific antibody is stably integrated into the genome of the mammalian cell and comprises in the 5' to 3' direction a first expression cassette encoding a first heavy chain, a second expression cassette encoding the first heavy chain, a third expression cassette encoding a first light chain, a fourth expression cassette encoding the first light chain, a fifth expression cassette encoding the second heavy chain, a sixth expression cassette encoding the first light chain or the second heavy chain or the second light chain, and a seventh expression cassette encoding the second light chain, wherein the first heavy chain The chain comprises, from N-terminus to C-terminus, a first heavy chain variable domain, a CH1 domain, a hinge region, a CH2 domain, a CH3 domain, a peptide linker, a second heavy chain variable domain and a CL domain, the second heavy chain comprises, from N-terminus to C-terminus, a first heavy chain variable domain, a CH1 domain, a hinge region, a CH2 domain and a CH3 domain, the first light chain comprises, from N-terminus to C-terminus, a first light chain variable domain and a CH1 domain, and the second light chain comprises, from N-terminus to C-terminus, a second light chain variable domain and a CL domain, wherein the second heavy chain variable domain and the first light chain variable domain form a first binding site, and the first heavy chain variable domain and the second light chain variable domain form a second binding site.

96 New PCT-Patent Application based on EP 24 186 635.9 Insempra GmbH Vossius Ref.: AG4141 PCT S3 Abstract The present invention relates to a linear double-stranded deoxyribonucleic acid (dsDNA) molecule comprising one or more Tus protein (Tus) binding site(s) at the 5'-terminus, one or more Lac repressor protein (LacI) binding site(s) at the 3'-terminus, and a segment comprising a DNA sequence of interest (DOI) between said Tus binding site(s) and said LacI binding site(s). The present invention further relates to a non-naturally or naturally occurring RNA molecule as encoded by or transcribable / transcribed from the linear dsDNA molecule of the invention, and to an expression DNA cassette comprising a promoter, an RBS, a GOI encoding a POI, and one or more LacI binding site(s), wherein said expression cassette does not comprise a terminator sequence between the 3´ end of said GOI and said one or more LacI binding site(s). The present invention also relates to a non-naturally or naturally occurring RNA molecule as encoded by or transcribable / transcribed from said expression cassette. The present invention further relates to method of protecting a linear deoxyribonucleic acid (DNA) molecule from exonuclease degradation by adding one or more Tus binding site(s) at the first terminus of the DNA molecule and adding one or more LacI binding site(s) at the other terminus of the DNA molecule. The present invention further relates to a method of synthesizing a protein of interest (POI) in a cell- free protein synthesis (CFPS) reaction mixture by using the (ds)DNA and / or RNA molecules of the invention, and also to a cell-free biological system or CFPS reaction mixture comprising the (ds)DNA, expression cassette and / or RNA molecules of the invention.

The invention discloses a preparation method and application of beta-aminobutyric acid for improving saline-alkaline tolerance of crops, and relates to the technical field of biochemistry. The invention provides a preparation method of beta-aminobutyric acid, which comprises the following steps: synthesizing a desoxyribonucleic acid sequence SEQ ID NO: 2 for coding transaminase according to an amino acid sequence shown in SEQ ID NO: 1, connecting the sequence shown in SEQ ID NO: 2 with a vector by using ligase, constructing a recombinant vector, introducing the recombinant vector into host bacteria to obtain recombinant bacteria, culturing, adding an inducer, and carrying out freeze-drying to obtain beta-aminobutyric acid. And collecting thalli to obtain the transaminase. The prepared beta-aminobutyric acid can improve the saline-alkali resistance of crops, increase the relative water content in saline-alkali stress plants, improve the lipid peroxidation capacity of the plants and effectively relieve the influence of saline-alkali stress on plant growth.

This application relates to a deoxyribonucleic acid and pseudovirus preservation solution, its preparation method and application. The deoxyribonucleic acid and pseudovirus preservation solution includes: a buffer solution with a final concentration of 5 mM to 50 mM, a surfactant with a volume percentage of 0.05% to 1.0%, trehalose with a final concentration of 50 mM to 500 mM, a metal ion chelator with a final concentration of 0.5 mM to 5 mM, betaine with a final concentration of 100 mM to 1000 mM, and mannitol with a mass percentage of 1% to 10%. Each specific component in the above-mentioned deoxyribonucleic acid and pseudovirus preservation solution cooperates with each other at a specific concentration, which has the effect of stabilizing the state and structure of nucleic acids. It can achieve the preservation of nucleic acids and pseudoviruses without ultra-low temperature, and can stably preserve nucleic acids and pseudoviruses for up to 12 months at 30°C without affecting subsequent detection tests.

The invention provides an invasive species identification method and device based on deep learning and DNA storage and electronic equipment, and relates to the technical field of invasive organism prevention and control, and the method comprises the steps: obtaining a to-be-identified invasive species image, and generating a first DNA sequence of a to-be-identified invasive species in the invasive species image through a deoxyribonucleic acid DNA encoder; obtaining a plurality of second DNA sequences to be hybridized from the invasive species database, and obtaining the hybridization yield of the first DNA sequence and each second DNA sequence by using a hybridization yield predictor; and determining the species category corresponding to the DNA sequence with the highest hybridization yield as the species category of the invasive species to be identified. According to the method provided by the invention, the strong feature extraction capability of deep learning is combined with the advantages of ultrahigh density and ultra-long stability of DNA as a data storage medium, so that end-to-end mapping and recognition from a species image to an exclusive DNA sequence thereof are realized.

The invention belongs to the technical field of organoid culture, and particularly relates to tissue digestive juice for organoid culture as well as a preparation method and application of the tissue digestive juice. According to the tissue digestive juice, a DMEM / F-12 culture medium which contains L-glutamine and does not contain 4-hydroxyethyl piperazine ethanesulfonic acid is used as a basic culture medium, calcium chloride, collagenase I type, collagenase III type, V type hyaluronidase, deoxyribonuclease I, 4-hydroxyethyl piperazine ethanesulfonic acid and Y-27632 are combined and have a synergistic effect, cell damage is reduced, meanwhile, the tissue digestive juice has the advantages that the tissue digestive juice can be used for improving the tissue digestive juice quality, and the tissue digestive juice has a good application prospect. The digestion time is shortened, the tissue digestion efficiency is improved, and the culture success rate of the organoid is improved.

The invention relates to the technical field of biological materials and aggregation-induced emission materials, and mainly relates to a fluorescent nucleic acid gel strong adhesive and a preparation method thereof. The fluorescent nucleic acid gel strong adhesive is prepared by the following steps: mixing double-chain desoxyribonucleic acid and a double-end quaternary ammonium salt surfactant containing a tetraphenyl ethylene structure in an aqueous solution system, and oscillating and mixing to form an ionic compound; and carrying out centrifugal separation, water washing purification and drying treatment on the obtained ion compound, and adding dimethyl sulfoxide to obtain the biological organic gel material. The fluorescent nucleic acid gel strong adhesive prepared by the preparation method disclosed by the invention has good biocompatibility and can generate high-strength adhesive property on various materials, and the maximum adhesive strength can reach 7.58 MPa. The fluorescent nucleic acid gel strong adhesive prepared by the invention can be applied to the fields of biological adhesion treatment and the like.

In an example, a deoxyribonucleic acid (DNA) sample is introduced to a flow cell including depressions and first and second transposome complexes immobilized within the depressions, whereby tagmentation generates respective DNA sample fragments in at least some depressions. The first and second transposome complexes respectively include first and second amplification domains. A pre-grafted particle suspension is introduced into the flow cell, whereby at least some pre-grafted particles respectively attach within a plurality of the at least some depressions. The suspension includes a liquid carrier and pre-grafted particles, which include first and second primers attached to a core. The first and second primers have a same sequence, respectively, as the first and second amplification domains. Amplification is initiated such that, within each of the plurality of the at least some depressions, the respective DNA sample fragments are amplified on a respective one of the at least some pre-grafted particles.

The invention relates to preparation and application of a desoxyribonucleic acid and desoxyribonucleic acid derivative transdermal delivery system, and belongs to the technical field of biological medicine and skin care products. Desoxyribonucleic acid and derivatives thereof have the effects of resisting aging, repairing and the like, but are difficult to penetrate through a skin barrier due to large molecular weight and strong polarity. According to the invention, hydroxyethyl chitosan, desoxyribonucleic acid and derivatives thereof are combined through electrostatic interaction to form the nano-composite. The compound is small in particle size and can promote transdermal absorption through hair follicle or cuticle gaps. The preparation method is simple, does not need an auxiliary reagent, and comprises the step of mixing a hydroxyethyl chitosan solution and a deoxyribonucleic acid derivative solution. Experiments show that the compound significantly enhances the transdermal efficiency, and has the effects of promoting cell repair and proliferation and resisting inflammation. The composition can be used for skin care products, and is high in safety and wide in application prospect.

A surface-enhanced Raman scattering (SERS)-based test kit for Staphylococcus aureus includes a magnetic nanosphere, gold / silver core-shell nanoparticles (Au—Ag NPs), Au nanoparticles (Au NPs), a SERS signaling molecule, a rolling circle amplification (RCA) template, a target aptamer, a complementary deoxyribonucleic acid (cDNA) and a single-stranded deoxyribonucleic acid (ssDNA). A detection method for S. aureus using the SERS-based test kit is also provided.

The present invention relates to methods for producing a protected deoxyribonucleic acid (DNA) product with enhanced resistance to nuclease digestion. The methods comprise digesting a double-stranded DNA molecule with an endonuclease to generate a digested double-stranded DNA molecule comprising a first strand and a second strand, wherein the first strand has a 5' overhang; and extending the second strand 5' to 3' using a polymerase in the presence of one or more nuclease-resistant deoxynucleotide triphosphates to generate the protected DNA product, wherein the protected DNA product comprises an extended second strand comprising at least one nuclease-resistant nucleotide incorporated into the extended second strand by the polymerase using the 5' overhang of the first strand as template. Also provided are protected DNA products and uses thereof.

The invention discloses functionalized adhesive hydrogel as well as a preparation method and application thereof, and belongs to the technical field of biomedical materials. The functional adhesive hydrogel is prepared from the following raw materials: recombinant humanized I-type collagen subjected to methacrylation, recombinant humanized I-type collagen modified by N-(2-aminoethyl)-4-(4-(hydroxymethyl)-2-methoxy-5-nitrophenoxy) butyramide, a photoinitiator and water, and the functional adhesive hydrogel is prepared from the following raw materials: recombinant humanized I-type collagen subjected to methacrylation, recombinant humanized I-type collagen modified by N-(2-aminoethyl)-4-(4-(hydroxymethyl)-2-methoxy-5-nitrophenoxy) butyramide, a photoinitiator and water; each milliliter of the functionalized adhesive hydrogel also comprises 80 to 120 U of deoxyribonuclease I, 400 to 600 ng of a clomastine free drug and 5 to 15 [mu] g of a clomastine liposome. The invention also discloses a preparation method and application of the functionalized adhesive hydrogel. The functionalized adhesive hydrogel provided by the invention can reduce secondary injury, inflammatory response and myelin sheath injury of a cerebral hemorrhage part, inhibit formation of neutrophil extracellular traps, and realize multi-level intervention after cerebral hemorrhage.

Disclosed herein are methods for processing a cell-free sample of a subject, comprising, providing said cell-free sample of said subject, wherein said cell-free sample comprises a plurality of nucleic acid molecules, subjecting said plurality of nucleic acid molecules to one or more amplification reactions to generate a plurality of complementary deoxyribonucleic acid (cDNA) molecules or derivatives thereof, and sequencing said plurality of cDNA molecules or derivatives thereof. Further disclosed herein are systems, comprising a processor, and a non-transitory computer readable storage medium encoded with a computer program that causes said processor to provide a cell-free sample of a subject, wherein said cell-free sample comprises a plurality of nucleic acid molecules, subject said plurality of nucleic acid molecules to one or more amplification reactions to generate a plurality of complementary deoxyribonucleic acid (cDNA) molecules or derivatives thereof, and sequence said plurality of cDNA molecules or derivatives thereof.

The present application relates to a deoxyribonuclease mutant and its application in preparing PDRN. The amino acid sequence of the deoxyribonuclease mutant is shown as SEQ ID NO. 1, and the nucleotide sequence of the encoding gene is shown as SEQ ID NO. 2. The present application also provides the application of the deoxyribonuclease mutant and / or the encoding gene of the deoxyribonuclease mutant in preparing PDRN, and the specific application method. The present application is based on the deoxyribonuclease expressed by the DNASE1 gene of bovine pancreas tissue, and the amino acids at positions 69, 132 and 215 are selected for iterative saturation mutation. Compared with the wild-type enzyme, the enzyme activity of the deoxyribonuclease mutant A69H-S132R-Q215C is increased by 2.15 times, reaching 4.1x10 6 U / mL, and the DNA extraction rate of salmon PDRN prepared by using the mutant enzyme prepared by the present application is 15.2%, the DNA content is 99.1%, and the molecular weight is 1.05 million Daltons, which is significantly improved compared with the wild-type enzyme, and the PDRN in this molecular weight range has more significant biological activity.

The application provides a kit for extracting RNA and application thereof. In the application, deoxyribonuclease (DNase) is used for DNA digestion in the process of magnetic bead extraction. The reaction environment can meet the optimal reaction environment requirement of the deoxyribonuclease (DNase), and at the same time, a hydrophobic environment is provided when the magnetic beads are adsorbed, so as to ensure the yield of nucleic acid. In addition, an emulsion of an oil is added to increase the total volume during the extraction operation, so as to facilitate the convenience of automatic extraction operation of the instrument. The extracted RNA has high yield and high purity, and the method can easily realize automatic extraction of the instrument. The requirement of extracting high-purity RNA quickly and conveniently in the process of clinical practical application is solved, the difficulty and cost of extracting high-purity RNA are greatly reduced, and the extracted RNA can be well adapted to subsequent RT-PCR and RNA-seq application.

The invention discloses a multi-channel high-precision liquid injection device for DNA synthesis and a method thereof, and relates to the field of deoxyribonucleic acid synthesis equipment.The multi-channel high-precision liquid injection device comprises a device shell; the movable mounting box is arranged at the bottom in the device shell in a sliding mode, a mounting groove is formed in the top of the movable mounting box, a clamping plate is arranged at the top in the mounting groove, and a plurality of combined columns are arranged on the clamping plate; and the cover plate is rotationally arranged on the fixed plate through a connecting rod. According to the invention, through the modularized liquid injection head assembly and the flexibly configured sequence identification system, the device can carry out multiple groups of different DNA synthesis operations at the same time, so that the synthesis flux and flexibility are obviously improved; by means of the driving and guiding mechanism and the electromagnetic coupling technology, efficient and accurate positioning of the liquid beating head in a two-dimensional plane is achieved, and the contradiction between multi-channel processing and positioning accuracy is solved.

The present invention relates to a functionalized artificial cell based on a composite coacervate, preparation and application thereof, and relates to the field of biomedicine technology. The preparation method is as follows: a positively charged solution and a negatively charged solution are mixed, the positively charged solution being a polylysine solution or an ε-polylysine hydrochloride solution, and the negatively charged solution being a deoxyribonucleic acid solution; a coacervate droplet suspension containing functional components is obtained by liquid-liquid phase separation; phospholipids and PEGylated phospholipids are dissolved in an organic solvent and then subjected to vacuum rotary evaporation until the organic solvent evaporates to obtain a lipid membrane; the coacervate droplet suspension is added to the lipid membrane for hydration until the lipid membrane is completely dissolved, thereby obtaining a functionalized artificial cell; at least one of the positively charged solution, the negatively charged solution and the phospholipid solution contains a functional component. The functionalized artificial cell based on the composite coacervate constructed by the present invention has a cell membrane-like and cytoplasm-like structure, and has good physiological stability and biocompatibility.

Methods for obtaining a target median deoxyribonucleic acid (DNA) insert size utilizes several solid-phase reversible immobilization (SPRI) bead solutions. At different steps of the method workflows, different ratios of the SPRI bead solution to DNA solution are utilized. Each method includes back-to-back DNA fragment removal / retention steps, where different ratios of the SPRI bead solution to DNA solution are utilized in the back-to-back steps.

A treatment method for room-temperature preservation of cell deoxyribonucleic acid, comprising the following steps: placing cells in a preservation tube containing a cell deoxyribonucleic acid preservation reagent, and performing freezing preservation at a temperature of -20°C to -80°C; and then performing thawing, and drying the cell deoxyribonucleic acid preservation reagent within the preservation tube to eliminate moisture.

Disclosed are single-stranded deoxyribonucleic acid (DNA) molecules comprising at least one nucleic acid sequence of interest flanked with a first at least one stem-loop structure, methods of making and methods of use for delivery and expression of a transgene in a host cell.

The invention provides a method for producing a partially closed deoxyribonucleic acid (DNA) product, wherein the method comprises: (a) contacting a double-stranded DNA molecule with an endonuclease a

The present disclosure relates to a method of inhibiting nuclease activity in a biological sample. In accordance with certain embodiments of the present disclosure, the method comprises mixing a biological sample with a metal-chelating agent complex. The present disclosure also discloses a method of isolating a cell nucleus from a cell in a biological sample by using the metal-chelating agent complex, and a method of adding a deoxyribonucleotide to the 3'terminal of a deoxyribonucleic acid.

Methods of nuclear targeted DNA delivery are provided. Aspects of the methods include contacting a cell with (a) a nuclear targeted deoxyribonucleic acid (NTDNA) comprising a DNA nuclear targeting sequence (DTS) and a cargo nucleic acid heterologous to the DTS. Compositions useful in practicing the methods of the invention are also provided.

Some embodiments of the present disclosure relate to one or more compositions that upregulate the production of one or more sequences of mRNA. The sequences of mRNA may encode for translation of a target biomolecule, thereby causing an increase in bioavailability of the target biomolecule within a subject that is administered the one or more compositions. In some embodiments of the present disclosure, the target biomolecule is a fusion protein with an Fc fragment, such as a toll-like receptor 3-Fc (TLR3-Fc). In some embodiments of the present disclosure, the target biomolecule is toll-like receptor 9-Fc (TLR9-Fc). In some embodiments of the present disclosure, the target biomolecule is deoxyribonuclease I-Fc (DNAse I-Fc). In some embodiments of the present disclosure, the target biomolecule is neural growth factor-Fc (NGF-Fc). In some embodiments of the present disclosure, the target biomolecule is insulin-Fc.

Herein is reported a method for producing C-terminally biotinylated FcRn comprising the steps of cultivating a mammalian cell comprising a deoxyribonucleic acid encoding FcRn and E. coli biotin-[acetyl-CoA-carboxylase] ligase (BirA) in a biotin containing medium, and recovering C-terminally biotinylated FcRn from the cell or the cultivation medium, wherein the deoxyribonucleic acid encoding FcRn and E. coli BirA is stably integrated into the genome of the mammalian cell and comprises in 5′- to 3′-direction a first expression cassette encoding class I major histocompatibility complex-like protein (α-FcRn) comprising a HisAvi-tag at the C-terminus, a second expression cassette encoding β2-microglobulin (β2m), a third expression cassette encoding class I major histocompatibility complex-like protein (α-FcRn) comprising a HisAvi-tag at the C-terminus, a fourth expression cassette encoding β2-microglobulin (β2m), and a fifth expression cassette encoding E. coli biotin-[acetyl-CoA-carboxylase] ligase.

A system includes a hydration module configured to rehydrate lyophilized reagents for a rolling circle amplification reaction. The system also includes a deoxyribonucleic acid (DNA) amplification module configured to generate a DNA product from a DNA template and rehydrated reagents utilizing a rolling circle amplification reaction.

Some embodiments of the present disclosure relate to one or more compositions that upregulate the production of one or more sequences of mRNA. The sequences of mRNA may encode for translation of a target biomolecule, thereby causing an increase in bioavailability of the target biomolecule within a subject that is administered the one or more compositions. In some embodiments of the present disclosure, the target biomolecule is a fusion protein with an Fc fragment, such as a toll-like receptor 3-Fc (TLR3-Fc). In some embodiments of the present disclosure, the target biomolecule is toll-like receptor 9-Fc (TLR9-Fc). In some embodiments of the present disclosure, the target biomolecule is deoxyribonuclease I-Fc (DNAse I-Fc). In some embodiments of the present disclosure, the target biomolecule is neural growth factor-Fc (NGF-Fc). In some embodiments of the present disclosure, the target biomolecule is insulin-Fc.