64 results about "DNA - Deoxyribonucleic acid" patented technology

96 New PCT-Patent Application based on EP 24 186 635.9 Insempra GmbH Vossius Ref.: AG4141 PCT S3 Abstract The present invention relates to a linear double-stranded deoxyribonucleic acid (dsDNA) molecule comprising one or more Tus protein (Tus) binding site(s) at the 5'-terminus, one or more Lac repressor protein (LacI) binding site(s) at the 3'-terminus, and a segment comprising a DNA sequence of interest (DOI) between said Tus binding site(s) and said LacI binding site(s). The present invention further relates to a non-naturally or naturally occurring RNA molecule as encoded by or transcribable / transcribed from the linear dsDNA molecule of the invention, and to an expression DNA cassette comprising a promoter, an RBS, a GOI encoding a POI, and one or more LacI binding site(s), wherein said expression cassette does not comprise a terminator sequence between the 3´ end of said GOI and said one or more LacI binding site(s). The present invention also relates to a non-naturally or naturally occurring RNA molecule as encoded by or transcribable / transcribed from said expression cassette. The present invention further relates to method of protecting a linear deoxyribonucleic acid (DNA) molecule from exonuclease degradation by adding one or more Tus binding site(s) at the first terminus of the DNA molecule and adding one or more LacI binding site(s) at the other terminus of the DNA molecule. The present invention further relates to a method of synthesizing a protein of interest (POI) in a cell- free protein synthesis (CFPS) reaction mixture by using the (ds)DNA and / or RNA molecules of the invention, and also to a cell-free biological system or CFPS reaction mixture comprising the (ds)DNA, expression cassette and / or RNA molecules of the invention.

The invention provides an invasive species identification method and device based on deep learning and DNA storage and electronic equipment, and relates to the technical field of invasive organism prevention and control, and the method comprises the steps: obtaining a to-be-identified invasive species image, and generating a first DNA sequence of a to-be-identified invasive species in the invasive species image through a deoxyribonucleic acid DNA encoder; obtaining a plurality of second DNA sequences to be hybridized from the invasive species database, and obtaining the hybridization yield of the first DNA sequence and each second DNA sequence by using a hybridization yield predictor; and determining the species category corresponding to the DNA sequence with the highest hybridization yield as the species category of the invasive species to be identified. According to the method provided by the invention, the strong feature extraction capability of deep learning is combined with the advantages of ultrahigh density and ultra-long stability of DNA as a data storage medium, so that end-to-end mapping and recognition from a species image to an exclusive DNA sequence thereof are realized.

The invention relates to preparation and application of a desoxyribonucleic acid and desoxyribonucleic acid derivative transdermal delivery system, and belongs to the technical field of biological medicine and skin care products. Desoxyribonucleic acid and derivatives thereof have the effects of resisting aging, repairing and the like, but are difficult to penetrate through a skin barrier due to large molecular weight and strong polarity. According to the invention, hydroxyethyl chitosan, desoxyribonucleic acid and derivatives thereof are combined through electrostatic interaction to form the nano-composite. The compound is small in particle size and can promote transdermal absorption through hair follicle or cuticle gaps. The preparation method is simple, does not need an auxiliary reagent, and comprises the step of mixing a hydroxyethyl chitosan solution and a deoxyribonucleic acid derivative solution. Experiments show that the compound significantly enhances the transdermal efficiency, and has the effects of promoting cell repair and proliferation and resisting inflammation. The composition can be used for skin care products, and is high in safety and wide in application prospect.

Disclosed herein are methods for processing a cell-free sample of a subject, comprising, providing said cell-free sample of said subject, wherein said cell-free sample comprises a plurality of nucleic acid molecules, subjecting said plurality of nucleic acid molecules to one or more amplification reactions to generate a plurality of complementary deoxyribonucleic acid (cDNA) molecules or derivatives thereof, and sequencing said plurality of cDNA molecules or derivatives thereof. Further disclosed herein are systems, comprising a processor, and a non-transitory computer readable storage medium encoded with a computer program that causes said processor to provide a cell-free sample of a subject, wherein said cell-free sample comprises a plurality of nucleic acid molecules, subject said plurality of nucleic acid molecules to one or more amplification reactions to generate a plurality of complementary deoxyribonucleic acid (cDNA) molecules or derivatives thereof, and sequence said plurality of cDNA molecules or derivatives thereof.

The present application relates to a deoxyribonuclease mutant and its application in preparing PDRN. The amino acid sequence of the deoxyribonuclease mutant is shown as SEQ ID NO. 1, and the nucleotide sequence of the encoding gene is shown as SEQ ID NO. 2. The present application also provides the application of the deoxyribonuclease mutant and / or the encoding gene of the deoxyribonuclease mutant in preparing PDRN, and the specific application method. The present application is based on the deoxyribonuclease expressed by the DNASE1 gene of bovine pancreas tissue, and the amino acids at positions 69, 132 and 215 are selected for iterative saturation mutation. Compared with the wild-type enzyme, the enzyme activity of the deoxyribonuclease mutant A69H-S132R-Q215C is increased by 2.15 times, reaching 4.1x10 6 U / mL, and the DNA extraction rate of salmon PDRN prepared by using the mutant enzyme prepared by the present application is 15.2%, the DNA content is 99.1%, and the molecular weight is 1.05 million Daltons, which is significantly improved compared with the wild-type enzyme, and the PDRN in this molecular weight range has more significant biological activity.

The application provides a kit for extracting RNA and application thereof. In the application, deoxyribonuclease (DNase) is used for DNA digestion in the process of magnetic bead extraction. The reaction environment can meet the optimal reaction environment requirement of the deoxyribonuclease (DNase), and at the same time, a hydrophobic environment is provided when the magnetic beads are adsorbed, so as to ensure the yield of nucleic acid. In addition, an emulsion of an oil is added to increase the total volume during the extraction operation, so as to facilitate the convenience of automatic extraction operation of the instrument. The extracted RNA has high yield and high purity, and the method can easily realize automatic extraction of the instrument. The requirement of extracting high-purity RNA quickly and conveniently in the process of clinical practical application is solved, the difficulty and cost of extracting high-purity RNA are greatly reduced, and the extracted RNA can be well adapted to subsequent RT-PCR and RNA-seq application.

The invention discloses a multi-channel high-precision liquid injection device for DNA synthesis and a method thereof, and relates to the field of deoxyribonucleic acid synthesis equipment.The multi-channel high-precision liquid injection device comprises a device shell; the movable mounting box is arranged at the bottom in the device shell in a sliding mode, a mounting groove is formed in the top of the movable mounting box, a clamping plate is arranged at the top in the mounting groove, and a plurality of combined columns are arranged on the clamping plate; and the cover plate is rotationally arranged on the fixed plate through a connecting rod. According to the invention, through the modularized liquid injection head assembly and the flexibly configured sequence identification system, the device can carry out multiple groups of different DNA synthesis operations at the same time, so that the synthesis flux and flexibility are obviously improved; by means of the driving and guiding mechanism and the electromagnetic coupling technology, efficient and accurate positioning of the liquid beating head in a two-dimensional plane is achieved, and the contradiction between multi-channel processing and positioning accuracy is solved.

Methods for obtaining a target median deoxyribonucleic acid (DNA) insert size utilizes several solid-phase reversible immobilization (SPRI) bead solutions. At different steps of the method workflows, different ratios of the SPRI bead solution to DNA solution are utilized. Each method includes back-to-back DNA fragment removal / retention steps, where different ratios of the SPRI bead solution to DNA solution are utilized in the back-to-back steps.

A treatment method for room-temperature preservation of cell deoxyribonucleic acid, comprising the following steps: placing cells in a preservation tube containing a cell deoxyribonucleic acid preservation reagent, and performing freezing preservation at a temperature of -20°C to -80°C; and then performing thawing, and drying the cell deoxyribonucleic acid preservation reagent within the preservation tube to eliminate moisture.

The invention provides a method for producing a partially closed deoxyribonucleic acid (DNA) product, wherein the method comprises: (a) contacting a double-stranded DNA molecule with an endonuclease a

A system includes a hydration module configured to rehydrate lyophilized reagents for a rolling circle amplification reaction. The system also includes a deoxyribonucleic acid (DNA) amplification module configured to generate a DNA product from a DNA template and rehydrated reagents utilizing a rolling circle amplification reaction.

The present disclosure relates generally to devices and methods for rapid and accurate diagnosis of single nucleotide polymorphisms. According to one or more embodiments, a system for identifying a single nucleotide polymorphism (SNP) comprises an oligonucleotide primer having a SNP recognition sequence, a fluorophore conjugated to the oligonucleotide primer at a conjugation site to form a fluorophore-conjugated oligonucleotide primer, a polymerase for extending the oligonucleotide primer to form a double-stranded deoxyribonucleic acid (DNA) amplicon upon binding to an oligonucleotide strand having a complementary sequence to the oligonucleotide primer, and wherein the fluorophore-conjugated oligonucleotide primer is the only primer in the system such that the fluorophore emits a fluorescent signal only when the fluorophore-conjugated oligonucleotide primer binds to a sequence specifically complementary to the SNP recognition sequence.

In an example deoxyribonucleic acid (DNA) enrichment method, a DNA sample containing a region of interest is tagmented using bead-linked transposomes. This generates bead-linked DNA fragments. A Cas nuclease / RNA guide complex is introduced to the bead-linked DNA fragments, which cleaves DNA fragments containing the region of interest. The Cas nuclease / RNA guide complex is denatured, which releases the DNA fragments containing the region of interest from the bead- linked transposomes. The DNA fragments containing the region of interest are introduced into a flow cell containing surface-bound transposomes. Other enrichment methods are also disclosed.

Synthetic molecular tags are placed on an item at various points in a supply chain to create a molecular record of movement through the supply chain. Associations between each unique synthetic molecular tag and individual locations in the supply chain are stored in an electronic record which may be maintained in the cloud. The synthetic molecular tags are collected from the item and sequenced to determine movement of the item through the supply chain by reference to the electronic record. The synthetic molecular tags can be used for identifying recalled items based on locations in the supply chain associated with a recall. The synthetic molecular tags may be polynucleotides such as deoxyribose nucleic acid (DNA). The item may be any type of item including food.

The invention discloses application of a CGAS-STING signal channel inhibitor in preparation of a medicine for preventing and treating hepatic ischemia-reperfusion injury, and relates to the technical field of biological medicine. According to the application, a cGAS-STING signal channel in macrophages is inhibited by using a cGAS inhibitor or an STING inhibitor, activation of double-stranded deoxyribonucleic acid released by a neutrophile granulocyte extracellular trapping net on a DNA induction channel is blocked, and the M1 type polarization level of the macrophages is reduced. The traditional Chinese medicine composition can effectively relieve liver inflammatory response and cell apoptosis, and a new path is provided for preventing and treating acute liver injury after liver transplantation or liver resection.

Linear DNA with improved resistance against exonucleases and methods for its production. The present invention relates to methods for producing a linear deoxyribonucleic acid (DNA) product (e.g., a closed linear DNA product) with improved resistance to nuclease digestion. The present invention relates to methods comprising the steps of: (a) contacting a double-stranded DNA molecule with an endonuclease, a ligase, and first and second terminal adaptor molecules and intermediate adaptor molecules to form a single contiguous aqueous volume; and (b) incubating the single contiguous aqueous volume to generate a linear DNA product (e.g., a closed linear DNA product). The present invention also relates to linear deoxyribonucleic acid (DNA) products (e.g., a closed linear DNA product) with improved resistance to nuclease digestion and uses thereof.

The invention discloses application of desoxyribonucleic acid-poly-L-lysine composite hydrogel in redistribution of endogenous exosomes and treatment of myocardial infarction, and the desoxyribonucleic acid-poly-L-lysine composite hydrogel comprises a DNA (deoxyribonucleic acid) skeleton and poly-L-lysine wrapping the DNA skeleton, the DNA skeleton contains a nucleic acid aptamer targeting exosome surface protein, and the desoxyribonucleic acid-poly-L-lysine composite hydrogel is used for capturing and releasing endogenous exosomes. According to the invention, the injectable desoxyribonucleic acid-poly-L-lysine composite hydrogel is used for treating myocardial infarction, and targeted enrichment and controllable release of endogenous exosomes are realized through the unique molecular design of the desoxyribonucleic acid-poly-L-lysine composite hydrogel; important breakthrough is achieved in the aspects of targeting, controllability, safety, clinical transformation and the like, and a brand new solution is provided for myocardial infarction treatment.

Disclosed is a method of determining whether a human subject is not a carrier of spinal muscular atrophy (SMA). This method includes the steps of (i) collecting a genomic deoxyribonucleic acid (DNA) sample from a human subject; (ii) screening the genomic DNA sample to determine the human subject's copy number of survival of motor neuron 1 (SMN1) gene and whether one of the copies of the SMN1 gene is positive for a polymorphism associated with non-carriers of SMA having two copies of the SMN1 gene; and (iii) determining the human subject as not a carrier of SMA if the human subject includes two copies of the SMN1 gene with one of those copies being positive for the polymorphism. Also disclosed is a method of determining whether an individual has a decreased risk of being a carrier of spinal muscular atrophy (SMA), where the individual is identified to have a decreased risk of being a carrier of SMA when the individual has two copies of the SMN1 gene with one of those copies being positive for the polymorphism.

The invention relates to the technical field of biological information processing, in particular to a cattle embryo pre-implantation genetic evaluation method and system based on whole genome sequencing, and the method comprises the following steps: obtaining whole genome sequencing data of a male parent and a female parent, embryo trophoblast live detection sequencing data and culture solution free desoxyribonucleic acid sequencing data; performing variation detection and haplotype phasing on male and female parent data to form haplotype data, and calculating a haplotype transmission posterior at an anchor point based on trophoblast data; performing genome segmentation based on reading depth observation and allele frequency observation of a trophoblast and a culture solution, and performing Bayesian inference by combining haplotype transfer posteriori as priori to obtain posteriori probabilities of aneuploid, copy number variation, chimera, pathogenic homozygosis and pollution events; and outputting a passing / rechecking / elimination conclusion, adding sequencing and updating the posteriori during rechecking until ending, and outputting a final conclusion and a genome breeding value, so that the evaluation reliability and the decision interpretability are improved.

The present invention provides compositions that modulate gut microbiota and / or the activity of deoxyribonucleases (e.g., in the gastrointestinal tract) and are useful in the treatment of diseases and disorders related to the activity of deoxyribonucleases including, for example, neurodegenerative, inflammatory, and metabolic diseases and cancer.

A method for producing deoxyribonucleic acid (DNA) includes providing a circular DNA template. The method also includes conducting in vitro an amplification reaction on the circular DNA template to generate a DNA product that is rolling circle amplified, wherein a reaction fold-amplification is minimized to enhance protein expression from the DNA product, and wherein a rolling circle amplification reaction is conducted with at least one of (i) the rolling circle amplification reaction being conducted at 15 degrees Celsius to less than 25 degrees Celsius to minimize the reaction fold-amplification, (ii) the rolling circle amplification reaction being conducted with an input concentration of the circular DNA template being greater than 1 nanogram per milliliter to minimize the reaction-fold amplification, or (iii) the rolling circle amplification reaction being conducted with input concentrations of deoxyribonucleoside triphosphates being titrated to 1.2 millimolar or less per deoxynucleotide to minimize the reaction-fold amplification.

The application discloses a kind of nanoparticles for removing lung mucus and its preparation method and application, it is related to the field of biological medicine.The nanoparticles of the application are composed of inner core and shell coated on the surface of the inner core, wherein the inner core contains citric acid and photoluminescent polymer-aliphatic polyester copolymer;The shell contains deoxyribonuclease and hydrophilic polymer.The nanoparticles provided by the application have excellent biocompatibility and multifunctional integration characteristics, which realizes effective penetration of lung mucus barrier, specific degradation of DNA network, down-regulation of pro-inflammatory factors and inhibition of mucus production in sequence through cascade response mechanism, breaking through the limitations of traditional single therapy.In addition, the in vivo experimental results show that it can significantly reduce the thickness of lung mucus and the protein concentration of lavage fluid, and effectively relieve airway obstruction, and can be used for removing lung mucus.

In an example deoxyribonucleic acid (DNA) enrichment method, a DNA sample containing a region of interest is tagmented using bead-linked transposomes. This generates bead-linked DNA fragments. A Cas nuclease / RNA guide complex is introduced to the bead-linked DNA fragments, which cleaves DNA fragments containing the region of interest. The Cas nuclease / RNA guide complex is denatured, which releases the DNA fragments containing the region of interest from the bead- linked transposomes. The DNA fragments containing the region of interest are introduced into a flow cell containing surface-bound transposomes. Other enrichment methods are also disclosed.

The embodiment of the invention relates to a method for methylation detection. The method comprises obtaining a methylated single strand from a cell free deoxyribonucleic acid (DNA) from a body fluid. The method further includes linking the double-ended linker to the single strand to generate a single strand including the double-ended linker, the double-ended linker including a single strand portion, the single strand portion including different proportions of unbalanced bases. The method further includes performing polymerase chain reaction amplification on the single strand including the double-terminal linker using the index primer and a polymerase to form a sequencing library. The method further comprises sequencing the sequencing library to determine the methylation level of the cell free DNA. By means of the method, the free DNA of the target cell can be rapidly screened out, methylation detection can be accurately carried out, a sample with the tumor proportion as low as 0.05% can be sensitively detected to be positive, the test set AUC of clinical detection is 0.9491, and good clinical detection performance is achieved.

Therapeutic compositions for treating a subject, the compositions including a polymeric nanoparticle, an active agent encapsulated within the polymeric nanoparticle, and a delivery vehicle for targeted delivery of the polymeric nanoparticle and encapsulated agent to a target tissue in the subject. The therapeutic compositions can be designed for targeting the destruction of a Nuclei neutrophil extracellular trap (NET) in a venous thrombus in a subject. The active agent can be an enzyme, including deoxyribonuclease 1 (DNase 1) enzyme. Methods of treating a subject using the therapeutic compositions are provided, including methods of treating venous thrombus, deep vein thrombosis, or a related blood clotting disease and / or condition.

The embodiment of the invention relates to a method for methylation detection. The method comprises obtaining a methylated single strand from a cell free deoxyribonucleic acid (DNA) from a body fluid. The method further includes linking the double-ended linker to the single strand to generate a single strand including the double-ended linker, the double-ended linker including a single strand portion, the single strand portion including different proportions of unbalanced bases. The method further includes performing polymerase chain reaction amplification on the single strand including the double-terminal linker using the index primer and a polymerase to form a sequencing library. The method further comprises sequencing the sequencing library to determine the methylation level of the cell free DNA. By means of the method, the free DNA of the target cell can be rapidly screened out, methylation detection can be accurately carried out, a sample with the tumor proportion as low as 0.05% can be sensitively detected to be positive, the test set AUC of clinical detection is 0.9491, and good clinical detection performance is achieved.

An example of a kit includes a library preparation fluid, a sample fluid, and an enrichment fluid. The library preparation fluid includes library preparation beads, where each library preparation bead includes a first solid support, and a transposome attached to the first solid support. The fluid includes a genomic deoxyribonucleic acid sequence. The enrichment fluid includes target capture beads, where each target capture bead includes a second solid support, and capture probes attached to the second solid support. Each of the capture probes includes a single stranded deoxyribonucleic acid sequence that is complementary to a targeted region of the genomic deoxyribonucleic acid in the sample fluid.

The invention discloses a prostate cancer invasiveness evaluation method fusing radiomics and a liquid biopsy marker. The method comprises the following steps: firstly, synchronously collecting prostate multi-parameter magnetic resonance image data and a blood sample of a patient; sketching a focus area from the image, extracting a large number of image omics characteristics, and quantitatively analyzing liquid biopsy markers such as circulating tumor desoxyribonucleic acid concentration, mutation frequency and the like from blood; secondly, screening out an image feature subset strongly associated with each molecular marker for each molecular marker by utilizing the correlation between the liquid biopsy marker and the imageomics features, and respectively training a primary prediction model; thirdly, splicing the prediction probability of each primary model with the original liquid biopsy marker to construct a brand new fusion feature vector; according to the method, deep complementary fusion of molecular information and image information is realized, and the accuracy and interpretability of distinguishing inert and invasive prostate cancers are remarkably improved.

Aspects of the disclosure include the manufacture of non-naturally occurring bacteria for perchlorate bioremediation. An exemplary method includes determining a source bacteria having one or more source genes which code for perchlorate reduction and chlorite dismutase and determining a target bacteria that does not naturally include the source genes. A first sequence of deoxyribonucleic acid (DNA) is identified in the source genes that codes, in the source bacteria, for an ordered set of amino acids whose linear sequence results in a protein which breaks down perchlorates and a second sequence of DNA is determined which, if inserted into the target bacteria, would allow the target bacteria to code for the ordered set of amino acids. A gene package is built by replacing the first sequence of DNA with the second sequence of DNA and the gene package is inserted into the target bacteria, thereby forming a non-naturally occurring modified bacteria.

A system includes a hydration module configured to rehydrate lyophilized reagents for a rolling circle amplification reaction. The system also includes a deoxyribonucleic acid (DNA) amplification module configured to generate a DNA product from a DNA template and rehydrated reagents utilizing a rolling circle amplification reaction.