125 results about "DNA - Deoxyribonucleic acid" patented technology

Methods and compositions are generally provided for treating metabolic disorders, e.g., obesity. One aspect discloses methods and compositions for obtaining a biological sample from the subject, evaluating the sample for the presence or absence of a genetic indicator, wherein the genetic indicator is selected from a single nucleotide polymorphism and a level of gene expression, and performing a first metabolic procedure if the genetic indicator is present, or performing an alternative second metabolic procedure if the genetic indicator is absent. One aspect discloses methods and compositions for obtaining a sample including deoxyribonucleic acids (DNA) from the subject, evaluating the DNA for an absence or presence of one or more genetic indicators and performing a first metabolic procedure or an alternative second metabolic procedure based on the absence or presence of the genetic indicator(s). Other aspects are also disclosed.

The invention discloses applications of a CRISPR / Cpf1 system in gene editing. CrRNA in the CRISPR / Cpf1 system is modified crRNA; the modification manner is desoxyribonucleic acid modification; the desoxyribonucleic acid modification method comprises the step of increasing 1-3 desoxyribonucleic acid at the 5' terminal and / or increasing 1-3 desoxyribonucleic acid at the 3' terminal of crRNA; and the desoxyribonucleic acid is deoxythymidine acid. The experiment proves that the crRNA formed through chemical synthesis and having the modification can cause the mutation of an hAAVS1 gene and a THUMPD3-AS1 gene when used for the AsCRISPR / Cpf1 system or the FnCRISPR / Cpf1 system, namely, the modified crRNA has certain gene editing capacity when used for the AsCRISPR / Cpf1 system or the FnCRISPR / Cpf1 system, and therefore, the modified crRNA has a significant application value in gene editing utilizing the CRISPR / Cpf1 system.

The invention can prepare the microarray of double -stranded DNA(ds DNA) on the surface of solid substrates. By this method, the probes of ds DNA can engomphosised in the nuclear factor kappa B(NF-kappa B) to high-throughpput detect NF-kappa B inside of caryon and screen ds DNA / NF-kappa B molecules interfering dsDNA / NF-kappa B interaction. First, depending on patent technic and others methods of this invention, the invention prepare the microarray of double -stranded DNA(ds DNA) on the surface of solid substrates to allow mounts of dsDNA explorer on the dsDNA micro embattle to link with locus. Second, allow dsDNA micro embattle to across and link with specifically cell nuckoproteides or NF-kappa pure albumen. Third, do qualitative determination or quantiative determination to NF-kappa albumen in special caryon by reaction of NF-kappa B with dsDNA as micro embattle butt.

The invention relates to a construction method of a human amniotic mesenchymal stem cell bank. The construction method comprises the following steps: taking a human amnion for detection, flushing and washing the human amnion with a phosphate buffered solution, then smashing the human amnion, diluting with the phosphate buffered solution, digesting with trypsin, digesting with collagenase IV and deoxyribonuclease I, and filtering to obtain a single-cell suspension; by adding human serum albumin, transferring, insulin and sodium selenite into a DMEM / F12 basal culture medium with the ratio of VDMEM to VF12 being 1 to 1, placing human amnion mesenchymal stem cells in an incubator under the serum-free condition, and then performing liquid exchange and culture transfer; subjecting the mesenchymal stem cells obtained through in vitro culture and proliferation to liquid nitrogen refrigeration, and preserving the cells according to the gender of newborn infants, the ABO / Rh type and the HLA type; establishing cell information files, so that the human amniotic mesenchymal stem cell bank is constructed. The method has the characteristics of no other animal derivation, wide source range and no ethic limitation. With adoption of the method, the human amniotic mesenchymal stem cells can be provided for cell therapy and other application.

The invention discloses an electrochemical sensor for detecting lead and a preparation method and application of the electrochemical sensor. The electrochemical sensor comprises a glassy carbon electrode, a signal amplifying device, a response probe and a target probe, wherein the surface of the detecting end of the glassy carbon electrode ismodified by ordered mesoporous carbon, and gold nanoparticles are deposited on the ordered mesoporous carbon; a hydrosulfuryl-modified capture probe is adsorbed on gold nanoparticles; the signal amplifying device includes the ordered mesoporous carbon with gold nanoparticles being deposited; methylene blue is adsorbed on the ordered mesoporous carbon with gold nanoparticles being deposited; the response probe is adsorbed on the gold nanoparticles of the signal amplifying device through hydrosulfuryl; the nucleotide sequence of the response probe is nucleotide sequence of chimera of deoxyribonucleic acid and nucleotide adenosine. The preparation method of the electrochemical sensor includes the steps of modifying the glassy carbon electrode, preparing the glassy carbon electrode and preparing the response probe and target probe solution. The electrochemical sensor provided by the invention can be used for detecting the lead ions in water, and has the advantages that the operation is simplified, the response is quick, and the sensitivity, the detection accuracy and the anti-jamming property are high.

The invention belongs to the biomedical field, discloses a double-stranded asymmetric small nucleic-acid-interference-molecule asiRNA inhibiting a tumour apoptosis suppressor specifically and an application thereof. The asiRNA is designed by a following method and obtained by chemical synthesis, starting from a Bcl 2 gene symmetry small nucleic-acid-interference-molecule siRNA, by cutting 1-5 nt base off a 5 ' end or a 3 ' end of an siRNA sense strand or an siRNA antisense strand, and forming the double-stranded asymmetric small nucleic-acid-interference-molecule asiRNA wherein base lengths are different and the 3' end of each strand is equipped with two deoxyribonucleic acids dTdT in a single strand suspension and RNA interference can be induced, by using two nucleotides with abutting deoxyribonucleic acid dTdT on the 3' end of each strand. The asiRNA of the invention is more stable than the routine siRNA, activity to inhibit expression of Bcl 2 is stronger, and can be applied in antitumor drug preparation.

The invention discloses DNA (desoxyribonucleic acid) fluorescent hydrogel and a preparation method thereof. The preparation method comprises steps as follows: (1) DNA hydrogel is prepared; (2) the DNAhydrogel is put in sterile water, water is changed once every 15-30 min, and water is changed 3-5 times in total; (3) the DNA hydrogel obtained in the step (2) is added to a phosphate buffer and an AgNO3 water solution for a reaction; a NaBH4 water solution is added, the mixture reacts, and the DNA fluorescent hydrogel is obtained. The DNA fluorescent hydrogel has good biocompatibility and material softness, can effectively avoid secondary injury of wound as medical dressing and can relieve pain of the wound; the hydrogel has fluorescence and has certain antibacterial property and no cytotoxicity. The hydrogel preparation process is simple and convenient. The hydrogel can be applied to the field of medical dressing, immunotherapy, tissue engineering, monocellular culture, drug sustained release, protein synthesis, biosensors and the like.

The invention discloses preparation and application of an adjacent hybridization dual-mode immunosensor based on MXene nanosheet photo-thermal amplification. Polyethyleneimine (PEI) modified TiO2 disc-shaped nanoparticles are used as a substrate, and a large amount of capture substrate deoxyribonucleic acids are fixed on a sensing interface through electrodeposition of gold. Two deoxyribonucleic acid probes are both labeled with a human epididymis protein 4 (HE4) antibody of a target object, and after the deoxyribonucleic acid probes as a recognition part of HE4 are captured by DNA3, the HE4 induces adjacent hybridization between primary antibody-labeled DNA1 and secondary antibody-labeled DNA2 by immunorecognition. An MXene nanosheet is loaded with a large amount of thionine, so that theMXene nanosheet can be effectively intercalated into a double-stranded DNA groove formed by hybridization, can be used as a signal probe for electrochemical-temperature dual modes, and realizes amplification of electrochemical signals due to temperature rise generated by the photo-thermal effect. Under the irradiation of an 808 nm infrared laser device, high-sensitivity detection of the HE4 is realized.

The present invention relates to a composition and method for treating cancer in the urinary bladder. More particularly, the present invention relates to a composition comprising a Mycobacterium phlei (M. phlei) deoxyribonucleic acid (M-DNA)-M. phlei cell wall complex (MCC), wherein the M-DNA is preserved and complexed on the M. phlei cell wall, and a pharmaceutically acceptable carrier. The MCC composition inhibits proliferation of and induces apoptosis in the cancer cells in the urinary bladder of the animal.

The invention relates to a multi-enzyme system for separating different tissues-derived primary culture cells of a normal human and a mammal. The system comprises a plurality of 0.0125 to 0.125 weight percent of trypsin, 0.02 to 0.2 weight percent of collagenase I-IV, 0.0015 to 0.015 weight percent of hyaluronidase, 0.02 to 0.2 weight percent of neutral protease, 0.0015 to 0.015 weight percent of deoxyribonuclease I, 1 to 10 U / ml papain, 0.015 to 0.15 weight percent of pronase, 5 to 50 U / ml elastase and 0.01 to 0.1 weight percent of separase. The invention also relates to the application of the multi-enzyme system and a kit formed by the multi-enzyme system. The multi-enzyme system of the invention has the characteristics of reasonable design and capacity of separating the different tissues-derived primary culture cells of the normal human and the mammal highly specifically and highly sensitively; and the separated primary culture cells have the characteristics of high survival rate, high purity, easy cryopreservation and recovery, can be used for medicament experimental evaluation, medicament toxicity test and medicament therapeutic judgment, and are suitable for large-scale popularization and application.

The invention discloses a mixed enzyme for skin tissue dissociation. The mixed enzyme is composed of collagenase II, trypsin and deoxyribonuclease I, wherein the concentration of the collagenase II is 2-4 mg / mL; the concentration of the trypsin is 1-3 mg / mL; and the concentration of the deoxyribonuclease I is 0.01-0.05 mg / mL. Besides, the invention further discloses a dissociation method for the skin tissue. The skin tissue is pretreated with a specific buffer solution, and tissue dissociation is conducted through the specific mixed enzyme, so that a skin tissue single-cell suspension with large number of single cells, good activity, few fragments and low clustering rate can be efficiently, rapidly and stably obtained. The screened and optimized mixed enzyme and concentration have higher pertinence and higher efficiency; more costs and time are saved compared with those in a method of combining a few collagenases in a broad spectrum; and a good tool is provided for single-cell related research of a skin tissue sample which is always difficult to dissociate.

The invention belongs to a cell DNA detection technology and in particular relates to a method for detecting 8-hydroxy-2'-deoxyguanosine (8-OHdG) and 2'-deoxyguanosin (dG) in cell DNA. The method can be used for quantitatively detecting the change of the content of 8-OHdG and dG in cell DNA, caused by exposure of harmful ingredients in cigarette smoke, so that the purpose of evaluating DNA oxidation damage caused by induction of the harmful ingredients can be achieved. According to the method, deoxyribonuclease I and alkaline phosphatase are used for hydrolyzing DNA in a cell, and deferoxamine mesylate is added into enzymatic hydrolysate, so that the oxidation of dG in the enzymolysis process is avoided; enzymatic hydrolysate is detected by HPLC-MS / MS; the cell DNA oxidation damage degree is evaluated according to the value of 8-OHdG / 10<6>dG. By virtue of the method, the interference of high-concentration metal ions and artificial dG oxidation is removed, and thus the result is relatively accurate; the method is low in detection limit, high in sensitivity, good in repeatability and high in recovery rate.

The invention discloses a synthesis method for synthesizing a fluorescence DNA-polyphenylene acetylene hydrogel, and the method adopts DNA as a hydrogen netlike frame to synthesize a novel DNA hybrid hydrogel together with polyphenylene acetylene by electrostatic interaction. The synthesis method is simple and mild in condition; the hydrogel can effectively coat the polyphenylene acetylene in the netlike frame of the DNA so as to prevent the polyphenylene acetylene from having contact with bacteria; the hydrogel can be applied to sterilization regulation and control; when the hydrogel is added with deoxyribonuclease, the netlike frame of the DNA is damaged to release polyphenylene acetylene, the dissociative polyphenylene acetylene can approach to the bacteria or enter the bacteria to generate singlet oxygen or active oxygen substance by the irradiation of white light, so that the bacteria are killed; and the deoxyribonuclease can be utilized to realize controllable sterilization.

Devices and methods for delivering a chimeric deoxyribonucleic acid (DNA) marking agent to a target and identifying the target from the chimeric DNA marking agent are provided. A delivery device may be a projectile, a spray canister or a wet / dry article. The chimeric DNA marking agent includes unique DNA fragments and may also include a fill material such as a liquid, a gas or a powder. The chimeric DNA marking agent may be combined with any combination of general marking agent, an inhibiting agent, an immobilizing agent, a weighting agent and a protective agent. The DNA marking agent may be analyzed using any of a hybridization method utilizing a labeled probe, a gel electrophoresis method, determining the base sequence to confirm a predefined DNA sequence, amplifying at least one of the unique DNA fragments and using a polymerase chain reaction (PCR) method.

The invention relates to a culture and cryopreservation method of amniotic mesenchymal stem cells. The culture method comprises amniotic tissue separation, amniotic mesenchymal stem cell separation, P0 generation amniotic mesenchymal stem cell culture and amplification culture. According to the culture method of the amniotic mesenchymal stem cells, at a separating stage of the amniotic mesenchymalstem cells, a special mixed enzyme digestive juice system (the final concentrations of components in the digestive juice are as follows: 1.5-2U / mL of neutral protease, 0.5mg / mL of deoxyribonuclease Iand 1mg / mL of collagenase IV) is adopted for digestion, so that the amniotic mesenchymal stem cells can be more effectively separated from amniotic tissues, and then the yield of P0 generation cellsis obviously improved. Compared with conventional two-step digestion, the culture method provided by the invention has the advantages that a one-step digestion method is adopted, operation is more convenient, and the cultured amniotic mesenchymal stem cells have good activity, high yield, high purity and good repeatability.

This disclosure is directed to a pharmaceutical composition comprises tumor inhibiting cells (TICs) and a method for producing the TICs. The tumor inhibiting cells can be derived from immune cells including T cells, NK cells, or a combination thereof, modified to have inactivated Cbl-b gene alleles and free from Cbl-b bio-function (Cbl-bv- / - TICs). The Cbl-b- / - TICs are free from deoxyribonucleicacids exogenous to the immune cells. The immune cells can be isolated using a portable cell isolation and modification device. This disclosure is further directed to a method for treating tumorous conditions in subjects. The pharmaceutical composition can provide a subject-specific tumor treatment.

The invention relates to a preparation method of levodopa nanoparticles and biosensing application of the levodopa nanoparticles, and belongs to the technical field of nano biosensing. Levodopa (L-DA)is used as a unique precursor to synthesize levodopa nanoparticles (LNDS) at room temperature, and the formed LNDS can quickly and effectively quench a dye-labeled oligonucleotide fluorescent probe (FAM-DNA) through electrostatic interaction. Complementary deoxyribonucleic acid (cDNA) or adenosine triphosphate (ATP) is competitively combined with a fluorescent probe, so that fluorescence is recovered, and an effective sensing platform for detecting biomolecules is constructed. In addition, the sensing platform can also perform high-sensitivity and selective detection on adenosine triphosphatein a complex system (human serum). The synthetic process of the levodopa nanoparticles is simple and environmentally friendly, the toxic and side effects on cells and organisms are low, the levodopananoparticles have a very strong quenching effect on dye-labeled oligonucleotides, the background fluorescence is greatly reduced, and the signal-to-noise ratio is increased.

The present invention discloses a deoxyribonucleic acid extraction device during a nucleic acid detection process. The deoxyribonucleic acid extraction device comprises a base seat, a stirring assembly is arranged at a top part of the base seat, the stirring assembly comprises a measuring cylinder, a rubber sleeve, a plastic cylinder, a glass rod, a circular plate, a rubber tube, curved rods, first supports, compression springs, curved plates, supporting rods, first pin shafts, first handles and hooks. The deoxyribonucleic acid extraction device during the nucleic acid detection process is simple in whole operation flow and high in work efficiency. Beneficial effects are as follows: through cooperation between the glass rod, the circular plate, a servo motor, the curved plates, the compression springs, the curved rods and a clamping rod, the deoxyribonucleic acid extraction device can realize automatic stirring at a uniform speed for a long time of cell sap, greatly reduces labor amount of operating personnel, guarantees that cell walls in the cell sap can be damaged completely, releases whole deoxyribonucleic acid, can avoid problems of glass rod rupture and measuring cylinder toppling while improving operating efficiency, and guarantees normal extraction work.

An example embodiment may involve (i) receiving, by a server device, deoxyribonucleic acid (DNA) information associated with a user; (ii) parsing, by the server device, the DNA information to identify one or more single nucleotide polymorphisms (SNPs)—(iii) determining, by the server device and based, on the identified SNPs, an endocannabinoid genotype of the user; (iv) determining, based on the endocannabinoid genotype of the user, a recommendation of one or more cannabinoid formulations; (v) transmitting, to a client device associated with the user, a web-based representation of a first graphical user interface; and (vi) receiving, from the client device, an indication to display a detailed representation of a particular cannabinoid formulation of the one or more cannabinoid formulations.

The invention discloses a system for simultaneously detecting matrix metalloproteinase-9 and matrix metalloproteinase-2, a preparation method thereof and application thereof. The system comprises a polydopamine nano microsphere-fluorescein modified nucleic acid aptamer nano sensor and deoxyribonuclease, and the sensor is formed by adding MMP-9-aptamer-FAM and MMP-2-aptamer-Texas Red into polydopamine nano microspheres. According to the invention, polydopamine nano microspheres with an excellent quenching effect are prepared, a PDANS-modified fluorescein nucleic acid aptamer nano sensor is constructed through non-public valence bond combination, DNase-I is added, aptamer is hydrolysed, an object to be detected is dissociated, cyclic reaction is carried out, the fluorescence signal amplification is realized, and the sensitivity of a system is improved. The system is simple in preparation, the reaction condition is mild, the cost is low, and a new tool and a new method are provided for simultaneous and rapid detection of the matrix metalloproteinase-9 and the matrix metalloproteinase-2.

The invention discloses an artery plaque digestion method capable of remarkably improving the quality of a single-cell suspension. The kit comprises a collagen hydrolase I, a collagen hydrolase II, acollagen hydrolase IV, a collagen hydrolase XI, calcium chloride, deoxyribonuclease I, a phosphate buffer salt solution, a bovine serum albumin solution and an RPMI 1640 cell culture solution. The invention also discloses an application prospect of the polypeptide in digesting plaque tissues. The invention is invented mainly for digestion treatment of plaque tissues. Through combination of severalenzymes, plaque tissue can be digested into a single-cell suspension with a relatively high activity proportion, and the biological reaction of the plaque tissue is researched on the basis of singlecells, so that the situation that a large number of calcified cell debris, tissue impurities and the like influence a single-cell sequencing machine or a subsequent data analysis result is avoided.

A SWCNT coated with a twist-strained double-stranded circular deoxyribonucleic acid (DNA) is provided, together with methods for preparing the coated SWCNT, for removing the DNA coating from the SWCNT, and for sorting SWCNTs using the DNA coating.

The invention relates to the liver-specific delivery and / or expression of an enzyme which has a deoxyribonuclease (DNase) activity for enhanced clearance of cell free DNA (cfDNA) accumulated in hepatic porto-sinusoidal circulation and the use of such liver-specific delivery and / or expression for treatment of various diseases and conditions, including cancer and neurodegeneration.

The invention belongs to the field of anti-counterfeiting, and discloses a method for detecting authenticity of a marked object by amplifying signals of a desoxyribonucleic acid (DNA) anti-counterfeiting maker by utilizing a loop-mediated isothermal amplification technology. The method does not need professional molecular biology instruments, such as PCR amplifiers, gel imaging systems and the like, and a user can directly determine the authenticity detection result by unaided eyes. Due to high sensitivity and specificity of the loop-mediated isothermal amplification technology, the method enhances the response accuracy of the detection, reduces the amount of DNA required by marking an object, thereby enhancing the difficulty for counterfeiting and cracking; and enven realized that the object can even be directly marked by DNA without medium protection. Thus, the invention provides an improved quick and convenient method for detecting a DNA anti-counterfeiting maker as well as a method of making an object with DNA without medium protection.

In order to simply obtain a methylated DNA sample, a method including bringing a sample which may include methylated DNA into contact with a protein capable of binding to methylated DNA to bind the methylated DNA in the sample to the protein, bringing the sample obtained into contact with at least one deoxyribonuclease to degrade DNA in the sample; and detecting methylated DNA which is not degraded by the deoxyribonuclease by the binding of the sample obtained to the protein is used. In the method, at least one type of deoxyribonucleases is a deoxyribonuclease different from a methylation-sensitive restriction enzyme capable of degradating a single stranded DNA.

The present invention relates generally to systems, methods, storage media, and software arrangements for genotyping and / or haplotyping a sequence of polymorphic genetic loci in a deoxyribonucleic acid (DNA) sample or identifying a strain variant from the DNA sample. Exemplary embodiments of systems, methods, storage media, and software arrangements may perform the optimization of the design of one or more microarrays, each containing a set of oligonucleotide probes capable of detecting one or more known genotypes and / or haplotypes at given polymorphic genetic loci or identifying the strain variant, by optimizing the set of oligonucleotides to be incorporated into the microarrays and by optimizing the arrangement of a set of oligonucleotides on the microarrays. The optimization may be achieved through the application of one or more optimization procedures. The instant invention may be useful in typing individuals at the HLA loci or other polymorphic genetic loci, or may be employed to quickly identify viral or bacterial pathogens from which genome sequence information is available.

The invention provides a preparation method of an earthworm extract for relieving cough, eliminating phlegm, anti-inflammation and anti-microbial. The extraction method provided by the invention is mainly to extract the earthworm homogenate through NaAC buffer solution of pH 4.8-5.4 and acetone. After repeated tests, the present invention optimizes the extraction process provided by the applicant of the present invention, which greatly shortens the preparation time of the earthworm extract from 42-45 hours to 15-18 hours; and the yield increases from 73% to 100%. 82%, the recovery rate of the total activity has been improved, the specific activity has been increased from 1141 to 4443, and the specific activity has been increased by 3.9 times; the purification factor has increased from 41 times to 158 times, and the purification factor of the deoxyribonuclease active ingredient has increased by nearly four times. The preparation method provided by the invention has simple process, low cost and is easy to be popularized and applied in industrialized production. The purity and activity of the product prepared by the preparation method provided by the invention are significantly higher than that of related similar products prepared by the existing method.