71 results about "Deoxyribonuclease" patented technology

The present invention is separating and culturing process of human amnion mesenchyme stem cell and its medical composition. The separating and culturing process includes digesting human amnion successively with trypsin, collagenase and deoxyribonuclease, and filtering to prepare single cell suspension; culturing in DMEM / F12 culture medium with VDMEM and VF12 in the equal ratio and containing ox embryo blood serum in 10-20 vol% and basic fibroblast growth factor of ultimate concentration 10-20 ng / ml inside a culture box at 37 deg.c, saturated humidity and CO2 in 5 vol%; and replacing liquid and culture passage to proliferate and purify human amnion mesenchyme stem cell. The process has wide material source no ethnic limitation and wide application foreground. The medical composition may be used in various kinds of treatment.

Methods and compositions for inhibiting and / or inactivating nucleases by employing nuclease inhibitors are provided. The nuclease inhibitors comprise anti-nuclease antibodies and non-antibody nuclease inhibitors. The anti-nuclease antibodies of the present invention may be a polyclonal or monoclonal antibodies, and may be anti-ribonuclease antibodies, anti-deoxyribonuclease antibodies, or antibodies to non-specific nucleases. A preferred embodiment comprises at least two nuclease inhibitors, and is referred to as a nuclease inhibitor cocktail. In some specific embodiments, the invention concerns methods of performing in vitro translation comprising obtaining a first nuclease inhibitor, which inhibitor is further defined as an anti-nuclease antibody, and placing the anti-nuclease antibody in an in vitro translation reaction. In many cases, the in vitro translation reaction comprises at least one nuclease, which may be a ribonuclease, a deoxyribonuclease, or a nonspecific nuclease. The invention also relates to kits for the performance of various microbiological procedures, which kits comprise the nuclease inhibitors described herein.

The invention discloses a method for safely and quickly extracting a genomic deoxyribose nucleic acid (DNA) from blood. The method comprises the following steps of: lysing a blood cell by using TritonX-100 and sodium dodecyl sulfonate (SDS), precipitating a protein with saturated NaCl, separating the protein through centrifugation, and reducing the mechanical shearing damage of a solution to the DNA; preventing and inhibiting the degradation of deoxyribonuclease (DNase) to the DNA; excluding the pollution caused by organic solvent, metal ions and other cnucleic acid molecules; and reducing the protein, polyoses, lipids and the like to the lowest degree, wherein the extracted DNA has certain length, and substances which inhibit enzymes exist in the genomic DNA after purification. The method for extracting the genomic DNA from the animal blood is harmless, quick and simple in operation.

The invention discloses a bacillus subtilis polygene editing and expression adjusting and controlling system based on CRISPR Cpf1. Through a constructed Cpf1 expression vector pHT-XCR6 and a constructed crRNA array expression vector pcrF11, complete knockout of 2 genes, basic group modification of 6 genes and knock-in of 1 gene can be completed at one time. The vector pHT-XCR6 contains NgAgo protein, and is used for promoting recA mediated homologous recombination. Besides, a vector pLCg6-dCpf1-remA (used for integral expression of a Cpf1 mutant dCpf1 inactivated by deoxyribonuclease and fusion protein of an activating transcription factor remA on a bacillus subtilis genome) and a vector pcra3 (used for integral expression of a crRNA array on the bacillus subtilis genome) are constructed,and can be used for performing transcription inhibition and activation on different genes. The invention further establishes an economic efficient crRNA array assembling method of which the name is SOMACA (Synthetic Oligos Mediated Assembly of crRNA Array), and through synthesizing short-single-strand DNA (60nt ), a needed crRNA array is inserted in the vector pcrF11 or pcra3 and is used for guiding Cpf1 or dCpf1-remA for gene editing and expression adjustment and control.

The invention relates to compounds, compositions and methods for biofilm disruption and prevention. In particular, the invention relates to pharmaceutical compositions for the disruption of biofilm and prevention of biofilm in patients. The invention also relates to anti-biofouling compositions for the disruption of biofilm and prevention of biofilm on surfaces. The invention also relates to the removal of biological material from surfaces. The compositions of the invention include microbial deoxyribonucleases.

The present invention belongs to the field of animal leather product for medicine. The method for preparing radiation sterilized allogenous acellular dermal matrix mainly comprises the following steps: preparing skin; cutting skin with machine for separating corium and epidermis and removing cell: first removing: cleaning with pure water after immersing the skin in 10-20% of parenzyme for 30-50 minutes in 36-40 DEG C; and second removing: alternately immersing the skin piece which has a water content of 10-30% after water logging in 10-20% of deoxyribonuclease and 10-301051632074f ribalgilase for 2-4 times in 36-40 DEG C, and immersing the skin piece in 0.5-1.5% of deoxyribonuclease or ribalgilase for 5-30 minutes in 36-40 DEG C for removing the substance which can initiate the recognition reaction of host cell in the corium; immersing the processed material in common fixing agent for 50-120 minutes; and radiating for sterilization, namely executing gamma-ray radiation sterilization through electron accelerator or Co-60.

The invention relates to a target protein-induced deoxyribonuclease (DNase) cycle generation-based homogeneous immunoassay method. A detection solution of the immunoassay method is prepared from an antibody 1-DNA1 conjugate, an antibody 2-DNA2 conjugate, hemin, exonuclease III and DNA3 / DNA 4 double-stranded DNA. When a target protein exists, the target protein can be immunologically recognized by the antibody 1-DNA1 conjugate and the antibody 2-DNA2 conjugate at the same time; based on an ortho effect, a complex is formed by hybridizing DNA1 and DNA2 and then has a chain substitution reaction with DNA3 on the double-stranded DNA, so that DNA4 rich in a G4 sequence is released and combines with the hemin so as to form G-quadruple chain / hemin DNase; in addition, the DNA3 reacting with the DNA1 and the DNA2 can be recognized and cut by the exonuclease III, so that the DNA1 and the DNA2 can undergo a chain substitution reaction with another DNA3, and another DNA4 is accordingly released to produce DNase; therefore, if repeating in this way, a large amount of DNase can be produced. The DNase can catalyze the color development of tetramethylbenzidine (TMB) and the luminescence of luminol, and can be used for carrying out sensitive quantitative analysis on the target protein by means of color comparisons and chemiluminiscence. The immunoassay method is simple to operate, rapid, sensitive and high in universality, and has a certain clinical application value.

The invention provides a DNA probe, a reagent kit and a method for detecting deoxyribonuclease. The hairpin-shaped DNA probe serves as a substrate of deoxyribonuclease, and a fluorescence group and a corresponding fluorescence quenching group are marked at the two ends or the interior of the probe respectively. When the structure of the probe is complete, the fluorescence group and the fluorescence quenching group are located on the same DNA molecule, excited fluorescence of the fluorescence group is absorbed by the fluorescence quenching group, and the fluorescence group does not emit fluorescence; when the probe is degraded by deoxyribonuclease, the fluorescence group and the fluorescence quenching group are separated, and the fluorescence group can emit fluorescence normally. Whether deoxyribonuclease exists in a reaction system or not can be judged as long as the intensity of fluorescence emitted from the reaction system is detected with a real-time quantitative PCR instrument. The method can be widely applied to quality detection on residues of deoxyribonuclease in biological products or quantitative detection of deoxyribonuclease.

The present invention provides, in one aspect, a method for expressing DNase in a plant. The method comprises growing a plant that has been transformed with a nucleic acid construct comprising a nucleic acid sequence encoding DNase under the control of a regulatory nucleotide sequence that regulates the transcription of said nucleic acid sequence in said plant.

The present invention concerns the use of a polypeptide having deoxyribonuclease (DNase) activity for preventing or reducing redeposition of soil on an item during a subsequent cleaning or laundering process. The invention further concerns a detergent composition comprising a polypeptide having deoxyribonuclease (DNase) activity and a method for preventing or reducing redeposition of soil on an item during a subsequent cleaning or laundering process.