9 results about "Deoxyribonuclease" patented technology

The invention relates to a normal temperature amplification-CRISPR Cas12a one-pot method detection system and a nucleic acid detection method based on the detection system. The detection system consists of a normal-temperature nucleic acid amplification system (unit I) which focuses on target signal amplification and a CRISPR Cas12a system (unit II) which focuses on target recognition and signal output. By means of a self-driven and deoxyribozyme-mediated reaction equilibrium transfer strategy, the equilibrium in the initial stage of the reaction tends to the unit I, and the equilibrium in the middle and later stages of the reaction tends to the unit II, so that the compatibility of the two units is remarkably improved. By means of the system, high-sensitivity and high-specificity portable one-pot detection of nucleic acid targets can be achieved.

The invention relates to gene therapy, and more specifically, to AAV gene therapy vectors containing a novel chimeric deoxyribonuclease (DNase) protein transgene and methods of treating ailments such as cancer and neurodegeneration.

The invention discloses Kocuria salsicia H1, which has a collection number of GDMCC NO:64604 in Guangdong Province Microbiological Culture Collection Center. The strain is applied in the fermentation preparation of meat products without adding nitrite. The catalase produced by the strain H1 can keep the meat products bright in color. The produced nitric oxide can promote the meat products to produce NO-Mb after fermentation, so that the meat products are bright pink. The strain has lipase activity, can hydrolyze fat, and promote the formation of specific aromatic substances. The strain can produce acetoin, a volatile compound with a special creamy aroma, which is helpful to improve the flavor of the fermented meat products. The safety evaluation experiment proves that the strain H1 has no coagulation and hemolysis effects, does not contain heat-resistant deoxyribonuclease, and does not produce bioamines, so it is safe to be used for fermenting meat products. The invention provides a new way for the fermentation production of meat products.

The invention belongs to the technical field of agricultural biological protection, and discloses a targeted screen tube-vector insect lysozyme-nano magnesium compound system and application thereof in prevention and control of areca yellows, the compound system comprises an in-plant treatment module and a propagation blocking module, the in-plant treatment module comprises a screen tube targeted delivery unit, and the propagation blocking module comprises a propagation blocking unit. The sieve tube targeted delivery unit is formed by combining betel nut sieve tube navigation peptide ArePEP1 and a lysozyme-nano magnesium complex through a non-covalent bond; the propagation blocking module comprises an antifeedant micro-capsule activated by salivary enzyme of a vector insect, and phytoplasma deoxyribozyme PdeA and an insect antifeedant are encapsulated in the antifeedant micro-capsule; the target sequence of the phytoplasma deoxyribozyme PdeA is 5 '-GGAAACAG-3'; the in-plant treatment module and the propagation blocking module are jointly loaded on the same delivery vehicle. According to the areca yellows prevention and control compound system and the application method, pathogens in plants can be synchronously inhibited, insect propagation can be blocked, and high-altitude accurate delivery can be realized.

The present invention provides a method for preparing a nutritional composition of xenogenic EVs from tissues / organs, comprising steps of collecting tissue / organ sample from fetal or adult mammal (101), keeping the collected sample in buffered saline in cold (102), mincing it into small pieces (103) and then mixing them with collagenase and deoxyribonuclease (104) followed by incubation and agitation (105), filtrating the incubated sample pieces for obtaining a filtrated liquid suspension containing extracellular fluid having EVs (106), differentially centrifuging the liquid suspension to remove remaining cells and tissue debris and obtain a supernatant (107), filtering the supernatant for collecting a crude fraction of large EVs (108) and centrifuging the supernatant for enriching it into a fraction of smaller EVs (109), characterized in that the separated EVs are fetal EV cargo or sinusoidal EVs directly isolated from the collected samples, wherein these EVs have regenerative potency, and the cells remain intact during EV isolation.

A pharmaceutical composition for topical wound treatment comprising one or more nitrogenous heterocyclic compound of 5 or 6 atoms with imide group; one or more deoxyribonuclease enzyme with activity pH between 4.5 and 6.5; and one or more carboxylic acid; kits and process to obtain this pharmaceutical composition and uses for wounds treatment.

The application discloses a specific RNA-m 1 The application discloses a chain deoxyribozyme probe for specifically recognizing RNA-m 1 The application discloses a chain deoxyribozyme probe for specifically recognizing RNA-m 1 The application discloses a chain deoxyribozyme probe for specifically recognizing RNA-m

The invention relates to gene therapy, and more specifically, to AAV gene therapy vectors containing a novel chimeric deoxyribonuclease (DNase) protein transgene and methods of treating ailments such as cancer and neurodegeneration.

The invention relates to the technical field of biological cells, and discloses digestive juice and a preparation method and application thereof as well as cell suspension and a preparation method thereof. The digestive juice contains I-type collagenase, II-type collagenase, III-type collagenase, IV-type collagenase, hyaluronidase, elastase, neutral protease and deoxyribonuclease. The cell suspension prepared from the digestive juice has the advantages of high cell viability and large number of living cells, and the method has the advantages of high preparation speed and high efficiency.