700 results about "Cytoplasm" patented technology

The invention discloses construction and application of an L-malic acid producing issatchenkia orientalis strain, and belongs to the technical field of microorganisms and the technical field of microbial fermentation and metabolic engineering. Through a metabolic engineering means, a cytoplasm metabolic pathway of an issatchenkia orientalis strain is transformed, and a pyruvate decarboxylase coding gene, glycerol dehydrogenase and lactic dehydrogenase are knocked out; a pyruvate carboxylase gene derived from aspergillus flavus, a malate dehydrogenase gene derived from corynebacterium glutamicum and phosphoenolpyruvate carboxylase derived from escherichia coli are screened and integrated, so that a cytoplasm rTCA cycle synthesis pathway is enhanced; inserting a dicarboxylic acid external transporter gene from schizosaccharomyces cerevisiae at a neutral site GD21, and knocking out endogenous dicarboxylic acid internal transporter; the L-malic acid yield of the modified yeast strain in the fermentation process is remarkably increased and reaches 140.16 g / L. The engineering strain provided by the invention has good industrial application potential in efficient L-malic acid production.

The present disclosure provides bispecific chimeric antigen receptors targeting CD20 and BCMA. The CAR may comprise an scFv targeting CD20 and an scFv targeting BCMA, a hinge region, a transmembrane domain, a co-stimulatory region, and a cytoplasmic signaling domain. The chimeric antigen receptors can be used to treat autoimmune disorders or cancer.

The invention relates to the technical field of biomedical engineering and digital image processing, in particular to a digital image enhancement method based on cytopathology. The method comprises the following steps: eliminating noise and artifacts caused by non-uniform dyeing, section folding and dust factors; the problem of dyeing difference caused by different scanning devices is solved; key areas including cell nucleuses and cell membranes are highlighted, so that the visual effect of a low-contrast image is improved; segmenting an area, including cell nucleuses and cytoplasm, of the cells, and extracting morphological characteristics; the detail resolution of a low-resolution image, especially the definition of a small cell structure, is improved; the brightness component Y is corrected to compensate for the difference of different dyeing methods and dyeing qualities in brightness response, then the chromatic value is adjusted according to the color correction factor to make the colors of the images more consistent, and finally, the images which are acquired by different dyeing methods and dyeing qualities and subjected to brightness and chroma correction are fused to make the color of the images more consistent. And a final corrected image is obtained.

The invention discloses a bionic nano-composite, a preparation method thereof and application of the bionic nano-composite in preparation of a product for treating atherosclerosis, and belongs to the technical field of medicine preparation. The bionic nano compound is a metal nano enzyme Ir-TiO2 coated by an M2 macrophage cell membrane. According to the invention, the surfaces of Ir-TiO2 nanoparticles are coated with M2 macrophage membranes, so that the nanocomposite with a bionic structure is prepared. After intravenous injection, the formed bionic nanocomposite can actively target endothelial cells in atherosclerotic lesions, and release Ir-TiO2 antagonism to endothelial cells in plaques and cytoplasm of inflammatory macrophages. After the Ir-TiO2 reaches the diseased region, the Ir-TiO2 can play an enzyme role to achieve an anti-inflammatory effect, and can promote inflammatory cells such as macrophages and smooth muscle cells at the diseased region to discharge cholesterol, reduce lipid deposition in the cells, reduce the generation of foam cells and promote the growth of plaques. The nanotherapy can effectively prevent the progress of atherosclerosis lesion inflammation and relieve atherosclerosis.

The invention relates to a cervical TCT image cell segmentation method based on improved U-Net, and belongs to the field of image processing technology and medical technology. The problems that complex morphological characteristics of cells are not fully considered and fine cell segmentation is insufficient in cervical cancer intelligent screening research based on cervical TCT images are solved. Comprising the following steps: preprocessing a cervical cell TCT image; constructing a segmentation network based on the EGE-UNet; evaluating the cell image segmentation model based on qualitative and quantitative evaluation; and obtaining a cervical cell segmentation result based on the evaluated model. According to the method, high-quality cervical TCT cell image segmentation and fine segmentation of cervical cell nucleuses and cytoplasm can be realized by using the EGE-UNet model, and related research of subsequent cervical cell intelligent diagnosis is facilitated; based on an accurate cell segmentation result, the method can assist in extracting various cell morphological characteristics, has clinical guiding significance, and provides more interpretable quantitative data for subsequent cervical cell research.

The invention provides a respiratory syncytial virus antigenic polypeptide, nucleic acid and a vaccine. Compared with the respiratory syncytial virus wild type Pre-F protein, the respiratory syncytial virus antigenic polypeptide or the immunogenic fragment of the respiratory syncytial virus antigenic polypeptide has the following changes: the 104th to 144th regions of the Pre-F protein are replaced with GS linker; two groups of disulfide bonds are introduced into S155C, S290C, A149C and Y458C; the S190F and the V207L are used for realizing cavity filling; and P102A, L373R, I379V, M447V, P102A, L373R, the C terminal of the respiratory syncytial virus antigenic polypeptide or the immunogenic fragment thereof comprises a cytoplasm tail region fragment, and the cytoplasm tail region fragment is a fragment derived from 3-10 continuous amino acid residues of a PreF protein cytoplasm tail region. According to the present invention, the pre-F immunogenicity and / or stability can be improved, and the protein expression level can be easily improved.

The present disclosure provides bispecific chimeric antigen receptors that target BCMA and CD19. The CAR may comprise an scFv targeting BCMA and an scFv targeting CD19, a hinge region, a transmembrane domain, a co-stimulatory region, and a cytoplasm signaling domain. Chimeric antigen receptors can be used to treat autoimmune disorders or cancer.

The invention relates to the technical field of image processing, and discloses a gynecological cell morphology intelligent identification method and system based on machine vision, and the method comprises the steps: collecting an original image of gynecological cells, carrying out the color normalization processing of the collected original image, carrying out the denoising processing through employing a wavelet threshold value, and carrying out the image enhancement through employing adaptive histogram equalization; according to the method, color consistency errors of different batches of dyed images are reduced, noise introduced in the image acquisition process is eliminated, cell structure details are reserved, and the accuracy of diagnosis is improved. And then image enhancement is carried out, the contrast ratio of cell nucleuses and cytoplasm is improved, so that the characteristics in the cells are more obvious, segmentation can be more accurately completed during subsequent image segmentation, the lesion level of the gynecological cells can be accurately identified, and the accuracy of an identification result is ensured.

The invention relates to dithiolane modified beta-cyclodextrin, a preparation method thereof and application of the dithiolane modified beta-cyclodextrin as a drug carrier. The method comprises the following steps: carrying out acylation reaction on beta-cyclodextrin and lipoic acid, and modifying different numbers of dithiolane on the beta-cyclodextrin by changing a feeding ratio; the beta-cyclodextrin has a plurality of hydroxyl groups, and the activity of the hydroxyl groups at the C6 position is relatively high. The hydrophobic cavity of the dithiolane-modified beta-cyclodextrin can be loaded with a hydrophobic small-molecule drug, and the hydrophobic small-molecule drug can be loaded into the dithiolane-modified beta-cyclodextrin under the condition of heating reflux to form a nano-drug. The disulfide bond on the surface of the nano-drug and sulfydryl on protein or lipid on the outer side of a cell membrane are subjected to dynamic covalent thiol exchange, so that the nano-drug enters cells through a thiol-mediated cellular uptake way, endosomes and lysosomes can be avoided, the drug is quickly and directly delivered to the cytoplasm, degradation of the drug in the lysosomes is reduced, and the drug delivery efficiency is improved. The utilization rate of small-molecule drugs is improved, side effects are reduced, and better curative effects are achieved.

The invention relates to the technical field of fluorescent dyes, in particular to a citric acid-based fluorescent dye for distinguishing dead cells from living cells as well as a preparation method and application of the citric acid-based fluorescent dye. The invention discloses a citric acid-based fluorescent dye for distinguishing dead cells from living cells. The citric acid-based fluorescent dye comprises at least one of a compound 1 and a compound 2, the citric acid-based fluorescent dye can specifically enter dead cells and specifically dye cytoplasm of the dead cells, so that the dead cells can be dyed or imaged. The citric acid-based fluorescent dye is difficult to dye living cells, so that dead cells and living cells can be quickly and accurately distinguished, and the citric acid-based fluorescent dye can be applied to the field of biological medicines.

The invention discloses a multimodal-based cell experiment image processing method and system and a storage medium, and the method comprises the steps: obtaining a single-channel gray image or a multi-channel fluorescence image containing cytoplasm and cell nucleus characteristics, carrying out the Gaussian filtering of a cytoplasm channel, and carrying out the median filtering of a cell nucleus channel; performing threshold segmentation on the preprocessed cytoplasm channel image by using a maximum between-class variance method to generate an initial cytoplasm mask; performing adaptive threshold segmentation on the preprocessed cell nucleus channel image to generate an initial cell nucleus mask; performing logic OR operation on the initial cytoplasm mask and the initial cell nucleus mask to generate an initial cell region; and finally, calculating each morphological parameter of each cell region and removing the cell regions of which the morphological parameters exceed a preset range to generate a segmentation result of the complete cell region, the morphological parameters and spatial distribution information. The problems that in the prior art, cytoplasm and cell nucleus noise processing segmentation precision is low, overlapped nucleus regions are difficult to separate, and morphological parameter analysis reliability is poor are solved.

The invention discloses a stem cell fusion degree detection method and system based on artificial intelligence, and a storage medium. The method comprises the following steps: carrying out image preprocessing on a cell microscope image; automatically calculating an optimal threshold value by using an image threshold value segmentation algorithm to obtain a cytoplasm mask; median filtering is carried out on the original image to reduce noise, then an adaptive threshold segmentation method is adopted, a local threshold is calculated according to local area gray level distribution, and a cell nucleus binary image is generated; performing connected region marking on the cell nucleus binary image, calculating the area attribute of each region, and performing filtering according to a cell nucleus removal ratio parameter to obtain a cell nucleus mask; performing logic OR operation on the cytoplasm mask and the cell nucleus mask to obtain a complete cell segmentation result; and calculating the fusion degree of the stem cells based on the cell segmentation result. Therefore, the problems of accuracy and consistency of judging the fusion degree of the stem cells by observing microscope images with human eyes in the prior art are solved, and accurate detection of the fusion degree of the stem cells is realized.

The invention provides an organic molecule cytoplasm delivery carrier as well as a preparation method and application thereof, the compound for cytoplasm delivery has the characteristic of fat-soluble aggregation, and can form electrostatic adsorption with small molecule protein goods such as RNase A (13.7 KDa), Trypsin (24KDa) and Saporin (32.8 KDa), medium molecule protein goods such as HRP (40KDa) and macromolecular protein goods such as beta-Gal (430KDa), so that the compound for cytoplasm delivery can be used for preparing a lipid-soluble protein carrier. And furthermore, transmembrane transportation is formed through the strong fat solubility property, so that the protection and intracellular delivery of carried protein molecules are realized. According to the delivery mechanism, cargo molecules can quickly penetrate through cell membranes, and carried protein molecules can enter cytoplasm to exert functions without release operation. Development of the organic molecule cytoplasm delivery carrier is expected to assist potential protein therapies with low bioavailability and off-target toxicity to complete clinical transformation, which is crucial to tumor therapy, gene therapy and the like requiring precise local administration.

The invention discloses a preparation method and application of a QS-21-polyamino acid nano vaccine, and belongs to the field of biomedical nano materials. The preparation method comprises the following steps: modifying gamma-PGA with phenylalanine ethyl ester (F) to obtain a polymer PF40 of which the F grafting rate is 40%; aiming at the characteristic that QS-21 is strongly combined with cholesterol, PF40 (PFC) grafted with cholesterol (C) is developed, and PFC wraps OVA and QS-21 through hydrophobic force to construct a nano vaccine OQPFC. The cholesterol on the PFC can reduce the combination of the QS-21 and the cholesterol on a cell membrane so as to reduce the biotoxicity of the QS-21, and the QS-21 loading efficiency of the nano vaccine is improved, so that a better treatment effect is achieved. The OQPFC can escape from lysosome through carboxyl protonation, and a large amount of QS-21 and OVA are released in cytoplasm, so that the synergistic cellular immune activation effect of the carrier adjuvant PFC and the saponin adjuvant QS-21 is realized.

The invention provides a targeting vector and a method for targeting a host cell, the vector comprises a first molecule combined with an endocytosis receptor of a target cell and a second molecule for promoting release of a substance carried by the targeting vector into cytoplasm, when the targeting vector is a virus vector, the first molecule is not a part of a virus envelope protein, and the second molecule is not a part of a virus envelope protein. The second molecules promote endosome escape or lysosome escape of the targeting carrier. According to the present invention, the first molecule is designed according to the endocytosis receptor of the to-be-targeted cell so as to target different types of cells, and after the vector is subjected to reasonable mutation, the infection of the cells not requiring the targeting can be avoided, and the accuracy of the vector can be improved.

The invention discloses a genetically engineered bacterium for producing tanshinol as well as a construction method and application of the genetically engineered bacterium, and the construction method comprises the following steps: (1) taking escherichia coli W3110 as an original strain, and reconstructing a tanshinol synthesis route in cytoplasm; (2) positioning flavin reductase from klebsiella pneumoniae and a 4-hydroxyphenylacetic acid-3 hydroxylase mutant in a periplasmic space by using periplasmic space positioning signal peptide sufI. The genetically engineered bacterium for producing the tanshinol disclosed by the invention has the advantages that the yield of the tanshinol reaches 2.8 g / L after being subjected to shake flask fermentation for 24 hours, and the yield of the tanshinol reaches 26.6 g / L after being fermented in a 5L fermentation tank for 48 hours.

The invention discloses a decafluorobiphenyl-based Tat47-57 binding peptide, the amino acid sequence of the binding peptide is shown as SEQ ID NO.1 or SEQ ID NO.2, the fourth amino acid and the eighth amino acid of the SEQ ID NO.1 are bound through decafluorobiphenyl, and the fifth amino acid and the twelfth amino acid of the SEQ ID NO.2 are bound through decafluorobiphenyl. In particular, the decafluorobiphenyl-based Tat47-57 binding peptides (P3 and P6) are efficient CPPs, capable of delivering a variety of cargo molecules into the cytoplasm of mammalian cells. Meanwhile, decafluorobiphenyl cyclization plays a key role in cellular uptake and endosome escape. The two peptides P3 and P6 show remarkable cell permeability, and are speculated to enter cells through gridding protein dependent endocytosis. They also show efficient endosome escape ability, and have successfully applied P3 and P6 as CPPs to deliver phospholipid peptides and avidin.

The invention relates to the field of image processors and biological medicines, and discloses an NK cell activity rapid detection method based on image processing. The method comprises the following steps: acquiring an unmarked time sequence phase image sequence of an NK cell and target cell co-culture system; performing cell instance segmentation to track individual cells; extracting a morphological dynamic characteristic parameter set of the target cell, wherein the morphological dynamic characteristic parameter set comprises a volume change rate, a phase gradient entropy, a cytoplasm phase fluctuation frequency and a nuclear region phase mean value; inputting the parameters into a pre-trained death state discrimination model, and outputting a death probability; and calculating a killing efficiency index based on the death probability evolution curve, and judging the activity level of the NK cells. The system comprises a phase image acquisition unit, a cell segmentation unit, a feature extraction unit, a death judgment unit and an activity judgment unit. Through unmarked imaging and deep learning fusion analysis, high-precision, real-time, quantitative and ultra-early NK cell activity evaluation is realized, and the method is suitable for clinical instant inspection and immunotherapy monitoring.

The invention relates to the technical field of medical examination, in particular to a method for calculating the definition of a leukocyte microscopic image based on deep learning, which comprises the following steps: preparing and expanding a data set of the microscopic leukocyte image, outputting a clear image and a blurred image, and enabling each image to have a corresponding definition evaluation index; performing multi-feature fusion definition evaluation on the microscopic leukocyte image based on comprehensive evaluation calculation of a Sobel operator, a Laplacian operator and information entropy; accessing multi-feature fusion definition evaluation into a deep learning model, and building a definition evaluation model; the definition evaluation model is trained, and in the training process, parameters of the definition evaluation model are adjusted by optimizing a loss function; the trained definition evaluation model is deployed in an automatic microscope system, and the problems that definition evaluation is carried out on the whole view, the definition evaluation algorithm is limited, and cell nucleus and cytoplasm characteristics are not distinguished in the prior art are solved.

The invention provides a cell protein degradation platform based on an artificial biological macromolecular aggregate. Specifically, the invention provides a PROTAC functional module based on an interworking nucleic acid skeleton, a PROTAC-aggregate complex (MLO-PROTAC), a kit, application and a preparation method of the PROTAC functional module, the PROTAC-aggregate complex (MLO-PROTAC) and the kit, and also provides a targeted protein degradation method. In a PROTAC platform built by the PROTAC functional module and the PROTAC-aggregate complex, a programmable nucleic acid component is used as a core assembly unit, and the functional module is enriched and spatiotemporal-spatial regulation is performed by using the polypeptide aggregate, so that the delivery efficiency and the cytoplasm exposure degree are remarkably improved while the universality is maintained, and the delivery efficiency and the cytoplasm exposure degree are remarkably improved. Therefore, a more effective target protein degradation way is provided for the field.

The present disclosure provides bispecific chimeric antigen receptors targeting CD20 and BCMA. The CAR may comprise an scFv targeting CD20 and an scFv targeting BCMA, a hinge region, a transmembrane domain, a co-stimulatory region, and a cytoplasmic signaling domain. The chimeric antigen receptors can be used to treat autoimmune disorders or cancer.

The invention discloses application of a CMIET protein and a coding gene thereof in improving manganese resistance of plants. The invention belongs to the technical field of biology, and particularly relates to application of CMIET protein and a coding gene thereof to improvement of manganese resistance of plants. The CMIET protein can regulate and control biological manganese resistance, and excessive manganese in Chlamydomonas chloroplast can be discharged to cytoplasm by regulating and controlling expression of the protein CMIET or regulating and controlling gene or regulating and controlling activity or content of the protein CMIET, so that balance of manganese ion concentration in the chloroplast is maintained, and the chloroplast is prevented from being affected by manganese toxicity; the normal photosynthesis capability of the cells is maintained, and the normal photosynthesis is ensured.

The embodiment of the invention provides an image virtual dyeing method and device based on deep learning and a related assembly. The image virtual dyeing method comprises the following steps: utilizing a preprocessed photoacoustic microscopic image and a Hamp; e, performing iterative training on a pre-constructed virtual dyeing model by the dyeing image to obtain a trained virtual dyeing model, and performing virtual dyeing on the new photoacoustic microscopic image by using the trained virtual dyeing model to obtain Hamp; e, dyeing the image of the style (i.e., pseudo Hamp; e, dyeing the image). According to the embodiment of the invention, the image segmentation module is embedded in the generator to help the generator to distinguish cytoplasm and cell nucleus, so that the virtual staining model is constrained, and the generator can better generate a Hamp conforming result; e, dyeing the morphological characteristics of the image to ensure the generated pseudo Hamp; the E dyeing image retains more useful cell nucleus information, and the pre-constructed virtual dyeing model reduces noise in the learning process and effectively prevents the problem of overfitting due to the fact that an image segmentation module is embedded in a generator.

The invention discloses an application of pentagalloylglucose (PGG) in preparation of a medicine for resisting doxorubicin cardiotoxicity. The method comprises the following steps: inducing doxorubicin (DOX), establishing a myocardial cell H9C2 injury model, and determining the DOX-induced myocardial cell injury minimum concentration; after 20 [mu] M of PGG is added into a treatment group, DOX-induced H9C2 cell apoptosis can be significantly improved. A mouse myocardial injury model is induced and established by DOX, and compared with a doxorubicin group, the left ventricular systolic fraction (FS%) and ejection fraction (EF%) of mice in the doxorubicin and pentagalloylglucose group are remarkably increased, the myocardial fiber structure is improved, the cytoplasm vacuolation is reduced, the serum cTnT and BNP levels are reduced, and the ROS level is reduced. The experiment proves that the pentagalloylglucose can obviously reduce the cardiotoxicity induced by the DOX.

The invention relates to the technical field of skin care products, and particularly discloses a bionic liposome for simulating intercellular lipid components and a preparation method of the bionic liposome. The bionic liposome for simulating the intercellular lipid component comprises the following components in parts by mass: 30-38 parts of a solvent; 35 to 43 parts of a humectant; 1.34 to 1.66 parts of grease; 2.4 to 2.6 parts of a lipid substance; 2.58 to 3.22 parts of an emulsifier; 18 to 26 parts of a skin conditioner; 4.9 to 5.1 parts of composite ceramide; 1 to 3 parts of phytosphingosine; 0.09 to 0.11 part of a sphingomonas fermentation product extract; and the emulsifier is a compound of olive alcohol, polyglycerol-10 stearate, sodium stearoyl glutamate and sodium lauroyl lactylate. The method has the advantage that ceramide is not easy to crystallize and separate out.

The invention discloses a liquid cell product, a preparation method thereof and a preserving fluid for preparing the liquid cell product, and belongs to the technical field of immunodetection. The dendritic polymer is creatively added into the preserving fluid for preparing the liquid cell product, the cell morphology in the liquid cell product can be improved, and a thermal acceleration test proves that the preserving fluid can maintain the cell morphology in the liquid cell product for a long time. When the preservation solution is used for preparing the liquid cell quality control product for immunodetection, quality control spots with complete circles can be formed after sample application on slides made of different materials, it can be obviously judged that cells in the liquid cell quality control product are dispersed very uniformly after immunohistochemical staining, and the preservation solution has very high application value.

The invention discloses a polypeptide, a polypeptide / siRNA compound and application thereof, and belongs to the field of biological medicine preparations. The amino acid sequence of the polypeptide disclosed by the invention is as follows: Nap-FFKYGFRRGHHHHRHHHR or Nap-FFKYGFRRGHHHHKHHK. A polypeptide / siRNA compound formed by the polypeptide and siRNA is uniform in particle size and good in stability, the hydrophobic end of the polypeptide / siRNA compound can respond to cathepsin B in lysosome to be assembled into nanofibers, a membrane of the lysosome is damaged, and then escape of the lysosome of the siRNA is promoted; after the siRNA / cationic peptide part is released into cytoplasm, the cationic peptide responds to the change of cytoplasm pH to reduce the electropositivity of the siRNA molecule, so that efficient cytoplasm delivery of the siRNA molecule is realized, and the siRNA molecule is promoted to play a silencing effect. The embodiment of the invention shows that the Pep / siRNA compound disclosed by the invention has relatively strong in-tumor retention capacity in a mouse body and can effectively inhibit the growth of breast cancer tumors.

The invention discloses a nano compound based on a CRISPR-Cas9 system and application of the nano compound. The nano compound comprises a histidine oligopeptide-cell penetrating peptide conjugate and an RNP compound composed of sgRNA of a target gene and Cas9 protein, and the histidine oligopeptide-cell penetrating peptide conjugate and the RNP compound are self-assembled under the action of zinc ions to form the nano compound. The nano-composite designed by the invention is simple in structure, and overcomes the defects of high cytotoxicity and large particle size (diameter gt; compared with the prior art, the compound has the advantages that the compound can overcome the defects of complex chemical synthesis, potential immunogenicity and the like, can be used for extracellular-cytoplasm-cell nucleus trinity antivirus, is high in virus removal efficiency and good in antivirus effect, and has high market value and development potential.

The invention provides a carrier material for supporting a protein drug. The carrier material comprises an inner core, a calcium carbonate coating layer coating the inner core and a polyethylene glycol-polyglutamic acid diblock copolymer chain segment anchored on the coating layer. The intracellular protein delivery carrier provided by the invention can effectively support various metal ions and protein drugs with the Fenton / Fenton-like reaction catalysis effect, and after the intracellular protein delivery carrier is endocytosed by cells, the protein drugs, calcium ions and the metal ions with the Fenton / Fenton-like reaction catalysis effect are released; metal ions can catalyze hydrogen peroxide in the endosome / lysosome to generate hydroxyl radicals with high oxidation activity, lipid molecules are oxidized, and a lysosome membrane is destroyed; the released calcium ions can cause mitochondrial calcium overload, improve the level of hydrogen peroxide in cells, promote generation of hydroxyl radicals in endosome / lysosome and damage of membranes of the hydroxyl radicals, induce efficient rupture of the endosome / lysosome and deliver protein drugs into cytoplasm so as to play the functions of the protein drugs.

The invention discloses a kit and application of the kit in predicting the curative effect of TROP2-ADC. The kit comprises a TROP2 detection reagent which comprises a molecule specifically combined with TROP2, and the molecule is modified with a first detectable marker; and the TROP2 positioning reagent comprises a color developing agent which is specifically combined with the cell microstructure. According to the kit, expression of TROP2 is positioned through combination of reagents, breast cancer patients are divided into a cell membrane expression group and a cytoplasm expression group, the patient in the cell membrane expression group has high internalization efficiency and good treatment effect on TROP2-ADC, and the patient in the cytoplasm expression group has low internalization efficiency and poor treatment effect on TROP2-ADC. Therefore, the method can be used for predicting the curative effect of the triple negative breast cancer patient on the TROP2-ADC.