263 results about "Periodontitis" patented technology

The invention relates to the field of biomedical materials, in particular to composite hydrogel as well as a preparation method and application thereof. The composite hydrogel prepared by the invention realizes step-by-step release and time sequence regulation of a polypeptide drug through the combined action of Gel-PBA and GelMA: the anti-inflammatory peptide (Ac2-26) is quickly released under the conditions of high ROS and low pH, so that inflammation and pyroptosis are reduced; osteogenic peptide (OGP) is continuously and slowly released, and osteogenic differentiation is promoted. The step-by-step release mechanism of the anti-inflammatory peptide and the osteogenic peptide can realize accurate drug release, and is helpful for synergistically regulating inflammation and promoting bone regeneration. The composite hydrogel is used as a biological medicine material, can realize timely anti-inflammatory effect and long-term osteogenesis in a complex pathological environment such as diabetic periodontitis, and has a good application prospect.

The invention discloses injectable hydrogel for photo-thermal adjuvant therapy of periodontitis and a preparation method, and belongs to the technical field of high polymer material preparation, the preparation method comprises the following steps: reacting MXene dispersion liquid with epigallocatechin gallate and MgSO4; then sequentially carrying out ultrasonic treatment, centrifugation, washing and freeze-drying to obtain a multifunctional nanosheet MP-Mg; adding the multifunctional nanosheet MP-Mg into an oxidized gellan gum aqueous solution, and carrying out a mixed reaction to obtain a solution A; mixing the quaternary ammonium salt chitosan aqueous solution and the carboxymethyl chitosan aqueous solution in equal volume, and reacting to obtain a solution B; and mixing the solution A and the solution B in equal volume for reaction. The hydrogel prepared by the preparation method disclosed by the invention has injectability, photo-thermal responsiveness, biological membrane penetrability, synergistic antibacterial property and long-acting anti-inflammatory property, and an innovative and practical photo-thermal adjuvant therapy platform is provided for clinical treatment of periodontitis.

The invention relates to the technical field of biological medicines, in particular to rhizoma drynariae extracellular vesicles for treating periodontitis by targeting macrophage ETS2 genes as well as a preparation method and application of the rhizoma drynariae extracellular vesicles. The extracellular vesicles are creatively extracted from the traditional Chinese medicine rhizoma drynariae by combining a differential ultracentrifugation method with a sucrose density gradient centrifugation method, the functions of the extracellular vesicles are studied, and it is found that the extracellular vesicles can effectively slow down alveolar bone resorption in a periodontitis state and reduce periodontal soft tissue inflammation and have a good treatment effect on periodontitis. It is found for the first time that the rhizoma drynariae extracellular vesicles can reduce the inflammatory ability of macrophages in an inflammatory state and can reduce expression of proinflammatory factors TNF-alpha and IL-6. The invention has important scientific research value and clinical significance for further development and application of various traditional Chinese medicine plant extracellular vesicles including rhizoma drynariae extracellular vesicles.

The invention relates to a pH-responsive copper hydrogen phosphate-loaded hydrogel with multiple enzyme-like activities and a preparation method and application thereof. The material mainly shows catalase-like activity in a neutral microenvironment, and can remove H2O2 and relieve oxidative stress; in a weakly acidic microenvironment, the compound mainly shows peroxidase-like activity and catalyzes H2O2 to generate. OH bacteria with higher toxicity; meanwhile, the inherent osteogenesis promoting performance of copper synergistically enhances bone regeneration, the biocompatibility, the oxidation resistance, the osteogenesis promoting capacity under the oxidative stress condition and the antibacterial efficiency under the weak acid condition of the SA / CuHP hydrogel are evaluated through in-vitro experiments, and a type II diabetes rat periodontitis model is established; the in-vivo antibacterial and osteogenesis promoting effects and the biological safety performance of the compound are verified, and a new strategy is provided for treatment of periodontitis with diabetes mellitus.

The invention provides application of polypeptide in preparation of a product for treating and / or preventing gingivitis and / or periodontitis. The polypeptide with the amino acid sequence shown as SEQ ID NO: 1 is adopted for treating a gingival epithelial cell inflammation model, it is found that the polypeptide can effectively reduce the expression level of inflammatory factors IL-8, IL-1beta and TNF-alpha, and it is indicated that the polypeptide can effectively treat and / or prevent gingivitis and / or periodontitis and is suitable for preparing products for treating and / or preventing gingivitis and / or periodontitis.

The invention provides a pharmaceutical composition as well as a preparation method and application thereof. The pharmaceutical composition comprises the following raw materials in parts by mass: 2-6 parts of radix paeoniae alba, 1-4 parts of ginseng, 1-4 parts of mint and 0.3-3 parts of liquorice. The pharmaceutical composition provided by the invention adopts the radix paeoniae alba, the ginseng, the mint and the liquorice, the mint and the liquorice are medicinal and edible traditional Chinese medicines, and the radix paeoniae alba and the ginseng can be widely applied in a health food raw material list. The pharmaceutical composition is a pure traditional Chinese medicine preparation, has significant antibacterial and anti-inflammatory effects, can effectively inhibit gingival bleeding, reduce dental plaque, reduce gingival sulcus bleeding index, gingival index and dental plaque index, and significantly reduce probe depth, and has a good curative effect. The composition has a remarkable treatment effect on gingivitis and periodontitis and is free of toxic and side effects.

The invention provides a Falcarindiol derivative, preparation thereof and application of the Falcarindiol derivative in preparation of medicines for preventing and / or treating bone diseases. The structural general formula of the Falcarindiol derivative is as shown in formula I in the specification. Experimental results show that the Falcarindiol derivative can significantly inhibit osteoclast differentiation, and is expected to be used for preparing novel drugs for preventing and / or treating osteoporosis, osteoporosis fracture, osteolysis, periprosthetic osteolysis, tumor bone metastasis, inflammatory bone loss, periodontitis inflammatory bone resorption and other bone diseases. # imgabs0 #

The invention relates to the technical field of micro-needle drug loading and releasing, in particular to a soluble micro-needle drug delivery system with an adhesive backing and a preparation method thereof.The micro-needle drug delivery system comprises a micro-needle tip and a backing part, and a micro-needle acts on a periodontal pocket, so that on one hand, the tip can rapidly dissolve and release loaded drugs; and on the other hand, the hydrogel backing layer with adhesion not only can be used for loading medicines to achieve the purpose of treatment, but also can be used for realizing the adhesion of the periodontal pocket and the dental surface, so that a favorable environment is provided for tooth tissue regeneration, the medicine delivery efficiency is enhanced, the treatment effect is improved, and the clinical treatment requirement of periodontitis is better met. The composite microneedle drug delivery system provided by the invention has double advantages of the microneedle and the hydrogel, realizes effective delivery of the drug at the periodontal part, is used for treating periodontitis, and has a wide application prospect.

The invention discloses hydrogel for periodontitis as well as a preparation method and application thereof, and relates to the technical field of medicines. The hydrogel disclosed by the invention is prepared from composite silver nanoparticles, phenylboronic acid grafted modified natural polymers, polyvinyl alcohol and epigallocatechin gallate, the composite silver nanoparticles comprise polydopamine, epigallocatechin gallate and a silver simple substance; the natural polymer contains a six-membered ring unit, and the six-membered ring unit contains a group which can be oxidized into an aldehyde group. The hydrogel can adapt to the oral environment, responsively releases silver and epigallocatechin gallate under ROS stimulation, has excellent anti-inflammatory and bactericidal effects, is good in photo-thermal activity, can cooperate with the photo-thermal effect to efficiently remove dental plaque, also has the effects of promoting M2 polarization and promoting alveolar bone regeneration, and is especially suitable for treatment of periodontitis.

The invention discloses a polylipoic acid-based elastic patch capable of inducing adhesion in a wet environment as well as a preparation method and application of the polylipoic acid-based elastic patch. The polylipoic acid-based elastic adhesion patch can be obtained by simply concentrating and evaporating a mixed solution of lipoic acid and methacrylated gelatin. Due to the introduction of a methacrylated gelatin polymer chain, the layered crystal structure of an original lipoic acid-based polymer is reduced, so that the water sensitivity of the lipoic acid-based polymer is reduced, the release time of lipoic acid active molecules is prolonged, and a small amount of layered crystal structures are reserved in the polylipoic acid-based elastic adhesion patch; therefore, non-adhesion and rigidity are realized in a dry environment. Once exposed to water or body fluid, the hydrophilic polymer backbone of the patch can quickly absorb moisture, the entry of water molecules destroys the layered crystal structure, resulting in softness and adhesion of the patch. In a rat model with ligation of periodontitis, the patch can effectively relieve inflammation and inhibit alveolar bone resorption.

The invention discloses a dual-network injectable hydrogel as well as a preparation method and application thereof. The hydrogel is methacrylated gelatin / oxidized hyaluronic acid loaded with metal polyphenol nano-particles, and the metal polyphenol nano-particles are copper-gallic acid nano-particles which contain quaternized chitosan coatings and are loaded with antibacterial peptide HHC36. After the hydrogel is injected into a periodontal pocket, the hydrogel can adapt to different defect shapes, and the CuGA-coated HHC36-coated QCS nanoparticles are released by responding to a periodontitis microenvironment, so that the effects of resisting periodontal pathogenic bacteria, removing local excessive ROS (reactive oxygen species), effectively promoting fibroblast proliferation, collagen deposition, extracellular matrix accumulation and angiogenesis and further promoting periodontal bone regeneration are achieved. The preparation process is simple, the injectable hydrogel is low in price and easy to obtain, the injectable hydrogel can be prepared through simple steps, and a new thought is provided for treatment of periodontitis.

The invention relates to the technical field of animal experiment auxiliary equipment, in particular to a periodontitis modeling mouth angle retraction device and equipment with fixed illumination and view amplification, and the device comprises a supporting and fixing mechanism, a mechanical transmission mechanism and a fixed expansion mechanism; the supporting and fixing mechanism is used for supporting and fixing the whole device and the rat to be experimented; through a fixed supporting plate, a movable threaded rod, a first movable half clamp and a second movable half clamp in the fixed expansion mechanism, the mouth corners of to-be-experimented rats with different sizes are retracted; the mechanical transmission mechanism is provided with a first rotating mechanical plate, a second rotating mechanical plate, a magnifying observation mirror and illumination light, so that a clear view field and sufficient and uniform light are provided for an operator. By means of the unique structural design of the oral corner retracting device, the stability, comfort, safety, observation conditions, operation efficiency and accuracy in the periodontitis molding process are remarkably improved, and the problems that an existing oral corner retracting device is poor in fixing effect, safety and operability are solved.

The invention relates to the field of intelligent detection, in particular to a periodontal bone loss positioning and grading system based on a deep learning CT image, and the system comprises the steps: obtaining image data of a periodontal panoramic film and an apical film, carrying out the classified storage, and marking key anatomical points; inputting the preprocessed image data into a deep learning model for training and verification to obtain a periodontal bone loss positioning detection model; and based on the key anatomical points positioned by the periodontal bone loss positioning detection model, calculating a typing evaluation ratio, and based on the typing evaluation ratio, carrying out quantitative typing and site deviation judgment on the periodontal bone loss. According to the method, the key anatomical points can be automatically identified and analyzed from the high-resolution CT slice image, and the diagnosis precision and efficiency are remarkably improved. According to the method, the severity of periodontitis can be quantitatively evaluated, the disease progress can be tracked in real time, and reliable data support is provided for formulating a personalized treatment scheme.

The invention discloses a periodontitis dynamic prognosis prediction model construction method and system based on survival analysis, and the method comprises the steps: collecting and preprocessing periodontitis patient data, and constructing a structured multi-modal clinical data set; the method comprises the following steps: randomly dividing a multi-modal clinical data set into a training set and a test set according to patient IDs, only on the training set, carrying out single-factor Cox proportional risk regression analysis on all patient level data and tooth level data together, and screening indexes significantly related to periodontitis progress risks; taking periodontitis progress time as a dependent variable, and using the training set and the screened indexes to construct a time-dependent Cox periodontitis dynamic prognosis prediction model; and introducing an SHAP algorithm to analyze a prediction result of the time-dependent Cox periodontitis dynamic prognosis prediction model. The method can be used for realizing dynamic, accurate and explainable prediction of the periodontitis progress risk.

The invention relates to a periodontitis recognition system based on periodontal photos, which is mainly used for automatically recognizing periodontitis through the periodontal photos. The system comprises five modules: an image acquisition and preprocessing module, a multi-instance learning model training module, a classification and diagnosis module, a system common user side and a system expert user side. According to the system, through an image processing and target detection technology, firstly, collected periodontal pictures are preprocessed, low-quality pictures are eliminated, and tooth areas are cut out; and then classifying the images by using a multi-instance learning framework in combination with the comparison loss, and identifying the risk level of periodontitis. The user side provides a visual diagnosis result, and the expert side provides more detailed analysis information and suggestions for experts. The periodontitis diagnosis precision can be effectively improved, the clinical workload can be reduced, early-stage automatic identification of periodontitis can be realized, and timely and effective treatment suggestions can be provided for patients.

The invention discloses a composite bone graft material based on bletilla striata polysaccharide and autologous tooth bone meal as well as a preparation method and application of the composite bone graft material. The preparation method comprises the following steps: preparing bletilla striata polysaccharide, namely BSP, from bletilla striata; the method comprises the following steps: pretreating extracted teeth, and crushing to obtain autologous tooth bone powder, namely AutoBT; dissolving the BSP in water, and uniformly stirring to obtain a BSP solution; the AutoBT is poured into the BSP solution, and after standing and stirring, an AutoBT solution loaded with BSP is obtained; and carrying out vacuum drying on the AutoBT solution loaded with the BSP, and carrying out ultraviolet sterilization to obtain the composite bone graft material. The material can be applied to repairing of oral cavity bone defects, bletilla striata polysaccharide has the effects of resisting inflammation, inhibiting bacteria, promoting osteogenesis and inhibiting osteoclast, the bone steady state unbalance state in the periodontitis environment is effectively reversed, meanwhile, autologous tooth bone powder accelerates bone tissue regeneration and integration by means of the natural bionic structure and high porosity of the autologous tooth bone powder, and the bone tissue regeneration effect is improved. The risks of postoperative infection and immunological rejection are reduced.

The invention provides a nano-enzyme with sterilization, tooth desensitization and remineralization functions as well as a preparation method and application thereof, and relates to the technical field of nano-enzymes. Berberine, hemin and calcium fluoride are used as raw materials and are self-assembled into the multifunctional BHCF nano-enzyme with sterilization, tooth desensitization and remineralization functions through multi-dimensional interaction of ionic bonds, Ca < 2 + > coordination bonds, pi-pi accumulation and hydrogen bonds. The BHCF nano-enzyme has a clear peroxidase-like and superoxide dismutase-like double-enzyme catalytic capability and a photodynamic effect, the antibacterial activity of the BHCF nano-enzyme is obviously activated under an illumination condition, and the sterilization rate can reach 99.995%; meanwhile, the BHCF nano-enzyme has a good immediate and lasting sealing effect, and has excellent acid resistance, wear resistance and remineralization capacity. The BHCF nano-enzyme provided by the invention has huge application potential in preparation of drugs for treating periodontitis.

The application discloses a kind of solid teeth pain-relieving and inflammation-clearing traditional Chinese medicine composition, it is related to traditional Chinese medicine product technical field.The composition is composed of gypsum, raw rehmannia, coptis, talc, ocher, radix cyathulae and salvia miltiorrhiza seven raw medicinal materials, wherein gypsum, raw rehmannia are monarch drug, clear and purge stomach fire, cool blood and nourish yin;Coptis, talc are ministerial drug, clear heat and dry dampness, lead heat down;Ocher, radix cyathulae are auxiliary drug, heavy town counteract, lead fire down;Salvia miltiorrhiza is servant drug, promote blood circulation and remove blood stasis, promote tissue repair.Modern pharmacological research shows that each drug of the present party has the effects of anti-inflammatory, analgesic, antibacterial, improving microcirculation and protecting periodontal tissue etc.Clinical observation shows that the pain of most patients caused by stomach fire attacking upward, gum redness and swelling, burning pain, bleeding is significantly reduced after taking 1-2 doses, redness and swelling gradually subside.Compared with existing Chinese patent medicines, the present application is simple in prescription, low in cost, has the dual effects of rapid pain relief and long-term tooth fixation, and is especially suitable for patients with recurrent toothache and early periodontitis.

The invention belongs to the technical field of biomedicine, and particularly provides a periodontitis related marker which comprises glycochenodeoxycholic acid. The concentration change of the periodontitis related marker provided by the invention is directly related to the pathological mechanism of periodontitis. As bound bile acid, the bile acid is slowly degraded in saliva. And the glycochenodeoxycholic acid in the saliva is synthesized from gingival fibroblasts, so that the influence of systemic inflammatory diseases is greatly eliminated. Glycine chenodeoxycholic acid in saliva is used as a specific biomarker, a non-invasive, high-sensitivity and high-specificity detection method, system and equipment are established, objective quantitative evaluation and dynamic monitoring of periodontitis activity are achieved, the hysteresis limitation of traditional diagnosis is broken through, a convenient and non-invasive accurate diagnosis tool is provided for clinic, and the clinical application prospect is wide. And the technical blank of periodontitis activity noninvasive diagnosis is filled.

The invention discloses injectable composite collagen dispersion liquid and hydrogel thereof, a preparation method and application of the injectable composite collagen dispersion liquid in preparation of drugs for treating periodontitis, the injectable composite collagen dispersion liquid comprises freeze-dried SIS collagen, itaconic anhydride and a photoinitiator, the mass ratio of the freeze-dried SIS collagen to the itaconic anhydride is 1: (1-5), a solvent is a phosphate buffer solution, the injectable composite collagen dispersion liquid is subjected to photoinitiation, and the injectable composite collagen is obtained. To form a hydrogel. The injectable composite collagen dispersion liquid has certain flowability, directly acts on a periodontal lesion of a patient through an injector, and forms hydrogel in situ through short-time photo-initiation, the mechanical properties of the injectable composite collagen dispersion liquid and the hydrogel can meet the mechanical requirements of periodontal tissues, and the injectable composite collagen dispersion liquid has the effect of promoting osteogenesis; therefore, the compound can be applied to preparation of drugs for treating periodontitis.

The invention discloses a histone-derived peptide nano-particle for targeted degradation of cytoplasm cGAS and a preparation method and application thereof, and is characterized in that a polypeptide compound is dissolved in a phosphate buffer, ultrasonic treatment and room temperature standing self-assembly are performed to form nano-particles, and centrifugal purification is performed to obtain the histone-derived peptide nano-particle A4-CMA NPs for targeted degradation of cytoplasm cGAS. The nano-particles (A4 at CMA NPs) can be combined with cytoplasm cGAS in a targeted manner and mediate the cytoplasm cGAS to degrade, so that the activation of a cGAS-STING pathway is blocked, periodontal tissue inflammation and alveolar bone resorption are effectively relieved, and a new strategy is provided for periodontitis treatment.

The application relates to the field of high polymer materials, in particular to an exosome composite material for treating periodontitis and a preparation method thereof. The exosome composite material comprises the following raw materials in parts by weight: 10-20 parts of a chitosan derivative, 10-20 parts of sodium hyaluronate, 1-5 parts of zein, 0.5-1 part of exosomes, 0.1-0.3 parts of phosphonodipeptide sodium and 60-70 parts of deionized water. The exosome composite material can inhibit periodontal pathogenic bacteria, reduce the expression of proinflammatory factors by inhibiting the signal pathways such as NF-kappa B, and thus relieve periodontal tissue inflammation.

The utility model discloses a periodontitis local washing and medicine applying device, which relates to the technical field of medical instruments and comprises an injection cylinder, a partition plate is fixedly connected in the injection cylinder and divides the inside of the injection cylinder into two medicine storage cavities, two pistons are arranged in the two medicine storage cavities in a sliding manner, and the two pistons are arranged in the injection cylinder in a sliding manner. First adjusting mechanisms are arranged in the two medicine storage cavities correspondingly, the two pistons move through the corresponding first adjusting mechanisms correspondingly, one end of the syringe is fixedly connected with a mounting frame, a driving mechanism is arranged in the mounting frame, and the two first adjusting mechanisms are both matched with the driving mechanism; and a second adjusting mechanism is arranged on one side of the driving mechanism. The motor is started to enable the second gear to rotate, then the first gear meshed with the second gear is driven to rotate, then the corresponding lead screw can rotate, and therefore the sleeve can drive the corresponding piston to move.

The invention relates to a dental and / or oral care product comprising between 0.0039% and 0.0078% by weight of nisin establishing and / or maintaining a pH of at least 4.5 in situ. Although said rather low nisin concentration does not provide an anti-bacterial effect, it is surprisingly and advantageously capable of maintaining stable pH values of at least 4.5, thereby preventing destruction of a subject's teeth enamel based on enamel demineralization effects at pH below 4.5, and said low nisin concentration advantageously further avoids dysbacteriosis, i.e. the oral microflora remains balanced, thus not leading to an increase of e.g. periodontitis associated bacteria.

The invention discloses a periodontitis marker and application thereof. Belongs to the technical field of biomedical detection. Experiments prove that the expression of the hsacirc0005737 in patient tissues, periodontal ligament stem cells in an inflammation microenvironment and periodontitis model mouse tissues is remarkably up-regulated, and the expression level of the hsacirc0005737 is positively correlated with the severity and activity of periodontitis. Therefore, detection of the expression level can be used for diagnosis, activity evaluation, curative effect monitoring and prognosis of periodontitis. In addition, the osteogenic differentiation capacity and inflammatory response of the periodontal ligament stem cells can be influenced by regulating the expression of the hsacirc0005737, so that the hsacirc0005737 can be used as a potential target for treating periodontitis. The invention further provides a primer sequence, a detection reagent combination and a kit for detecting the hsacirc0005737, and a new solution is provided for noninvasive, early-stage and high-specificity diagnosis and targeted therapy of periodontitis.

The present invention relates to compositions for oral hygiene comprising Crocus sativus flower extracts and their uses for protecting the oral cavity. The invention also relates to therapeutic compositions for use in the prevention and treatment of diseases of the oral cavity, in particular periodontitis and gingivitis.

Disclosed is a selenium-containing polyamino acid having a structure represented by formula (I) or a pharmaceutically acceptable salt thereof, wherein A, B, D, E, m, n, and x are as defined in the description. Also disclosed is a pharmaceutical composition containing the selenium-containing polyamino acid or a pharmaceutically acceptable salt thereof. Also disclosed is a use of the selenium-containing polyamino acid or the pharmaceutically acceptable salt thereof, or the pharmaceutical composition containing the selenium-containing polyamino acid or the pharmaceutically acceptable salt thereof in the preparation of a drug for treating diseases selected from osteoarthritis, rheumatoid arthritis, and periodontitis. E[-(B)m-(A)n-D]x Formula (I)

This invention discloses a histone-derived peptide nanoparticle targeting the degradation of cytoplasmic cGAS, its preparation method, and its applications. The invention is characterized by: dissolving a peptide complex in phosphate buffer, sonicating, allowing it to self-assemble at room temperature to form nanoparticles, and purifying by centrifugation to obtain histone-derived peptide nanoparticles A4@CMA NPs targeting the degradation of cytoplasmic cGAS. These nanoparticles (A4@CMA NPs) can target and bind to cytoplasmic cGAS, mediating its degradation, blocking the activation of the cGAS-STING pathway, effectively reducing periodontal inflammation and alveolar bone resorption, and providing a new strategy for the treatment of periodontitis.

The invention belongs to the technical field of medicines, and discloses application of protein nano-selenium in preparation of a medicine for preventing and treating bone defects. Compared with nano-selenium particles prepared by a traditional method, the protein nano-selenium disclosed by the invention is better in antibacterial effect, higher in oxidation resistance and osteogenic induction capability and better in treatment effect on bone defect diseases such as periodontitis (such as diabetes mellitus complication), osteoporosis and the like.

An in vitro method for assessing whether a human patient has periodontitis is disclosed. The method is based on insight to determine biomarker proteins. Therefore, in a saliva sample of a patient suffering from periodontitis, the concentration of free light chain κ protein and / or free light chain λ is measured. Based on the measured concentration, a value reflecting the concentration or combined concentration for one or both of the proteins is determined. The value is compared with a threshold value reflecting the concentration or combined concentration associated with periodontitis in the same manner. The comparison allows an assessment of whether the test value indicates the presence of periodontitis in the patient. Thus, typically, a test value reflecting a concentration or combined concentration below the concentration or combined concentration reflected by the threshold indicates the absence of periodontitis in the patient, while a test value reflecting a concentration or combined concentration at or above the concentration or combined concentration reflected by the threshold indicates periodontitis in the patient.