79results about How to "High bone repair performance" patented technology

The invention relates to a piezoelectric active bone repair composite material and a preparation method thereof, aiming at solving the problems that the existing material does not have biocompatibility and is poor in piezoelectric property. The piezoelectric active bone repair composite material comprises ceramic particle filler with a core-shell structure, and polymer matrix, wherein the ceramic particle filler with the core-shell structure is evenly dispersed in the polymer matrix; the core body of the ceramic particle filler with the core-shell structure is formed by ceramic particles, and the surfaces of the ceramic particles are covered by organic matter coating layers. The invention also provides the preparation method of the piezoelectric active bone repair composite material. The preparation method can be widely applied to the field of preparation of the bone repair composite material.

The invention discloses a silk fibroin base integrated osteochondral two-layer bracket, preparation and application thereof. The preparation of the silk fibroin base integrated osteochondral two-layer bracket is carried out by the following steps of: respectively soaking parts of 1/2-3/4 height of a three-dimensional silk fibroin bracket in a calcium chloride ethanol solution, absolute ethyl alcohol, a dipotassium phosphate aqueous solution and de-ionized water; repeating the above operation for 1-15 times, taking the silk fibroin bracket finally soaked by the de-ionized water to soak in the calcium chloride ethanol solution for 5-30 min and subsequently using the de-ionized water to soak the silk fibroin bracket for 3-10 min and pre-calcify the three-dimensional silk fibroin bracket; then soaking the pre-calcified part of the three-dimensional silk fibroin bracket in a bionic calcium ion buffer solution and culturing for 4-8 days at a constant temperature of 37 DEG C to obtain the silk fibroin base integrated osteochondral two-layer bracket. In the pre-calcification process of the invention, the silk fibroin bracket can obtain a nucleating locus of hydroxyapatite crystals; and evenly dispersed weak crystallization nano hydroxyapatites are formed on the surfaces of the porous channels of the bracket by culturing the bracket in the bionic calcium ion buffer solution.

The invention discloses a preparation method of an antibacterial stent for bone repair. Firstly, gelatin and sodium alginate are dissolved in ultrapure water to obtain gelatin/sodium alginate slurry;58 s bioactive glass and the gelatin/sodium alginate slurry are mixed and uniformly stirred, the mixture is placed in a 3D printing barrel for defoaming to obtain gelatin/sodium alginate/bioglass composite slurry; the slurry is printed into a stent adopting a 3D porous structure by a 3D printer, and freeze drying treatment is performed to obtain a gelatin/sodium alginate/bioglass composite bone repair stent; the composite bone repair stent is placed in a polydopamine solution for reaction overnight, and placing a product in a silver nitrate solution after reaction; the stent is washed by deionized water, and freeze drying is carried out to obtain the antibacterial stent for bone repair. The antibacterial stent can have antibacterial and anti-infective functions while performing bone repair, the materials are widely sourced, the materials are good in mechanical properties and high in bioactivity, and the preparation process is simple and easy to operate and has broad application prospects in fields of regenerative medicine and bone repair.

The invention discloses a composite hydrogel for promoting bone repair. The composite hydrogel is prepared from the following components; methacrylic acid modified gelatin and dopamine modified sodiumalginate. In the composite hydrogel, the content of the methacrylic acid modified gelatin is 8-12% w/v, and the content of the dopamine modified sodium alginate is 0.5% w/v-2% w/v. A molecular structure of the dopamine modified sodium alginate contains a catechol structure, and the adhesion property is excellent. The composite hydrogel uses the methacrylic acid modified gelatin as a substrate ofthe hydrogel, and the mechanical properties and adhesion properties of the hydrogel are improved by incorporating the dopamine modified sodium alginate, so that the viscosity is excellent, the softness degree and elasticity are suitable, and the composite hydrogel is suitable for bone repair treatment. The results of in vitro experiments show that the composite hydrogel can load and slowly releasebiologically active factors and/or drugs that promote bone repair, good repair effect can be presented in bone defect models, and the healing of bone defect wounds can be significantly promoted.

A sleeve-type superfine diameter osteofixation needle comprises a power assisting sleeve and a superfine diameter osteofixation needle disposed in the power assisting sleeve, one end of the power assisting sleeve being provided with an adapter seat to connect to a rotor of a drive electric motor, and another end is provided with a detachable rigid connection apparatus. The power assisting sleeve is fixed to the osteofixation needle by means of the rigid connection apparatus, and the rigid connection apparatus limiting extension of a needle tip of the osteofixation needle from the power assisting sleeve to a set length. Starting the electric motor can cause the electrically driven osteofixation needle to enter a bone by means of percutaneous self-tapping drilling, and after the needle tip of the osteofixation needle extending from the power assistance sleeve penetrates a target bone, detaching the rigid connection apparatus from a distal end of the power assistance sleeve, removing thepower assistance sleeve from the osteofixation needle; finally, screwing into an anchor bone matrix by means of needle-holding forceps gripping a screw thread section of a back part of the osteofixation needle. Feasibility of percutaneous anchor needle puncturing is realized for small animal bone fracture and bone malformation models and clinical osteofixation of small bone breaks in specific parts, including hand and foot; the method is simple and convenient, time-saving and efficient, causes little surgical trauma, and needle anchoring stability is reliable.

The invention belongs to the field of composite materials, and concretely relates to a nano-hydroxyapatite/carboxymethyl chitosan/poly(lactic-co-glycolic acid) micro-nano hybrid drug-loaded scaffold and a bionic preparation method thereof. The three-dimensional bionic hybrid drug-loaded scaffold is prepared through combining solvent evaporation and freeze drying technologies by using a high speed emulsifying machine via adopting nano-hydroxyapatite and carboxymethyl chitosan as a water phase and an emulsion continuous phase, adopting a methylene chloride solution of poly(lactic-co-glycolic acid) as an oil phase and an emulsion dispersion phase, using glutaraldehyde as a cross-linking agent to immobilize the carboxymethyl chitosan in the continuous phase and adopting the main component icariin in a traditional Chinese medicine epimeddium as a carrier drug. The preparation method has the advantages of simple preparation process and mild reaction conditions, and the obtained scaffold overcomes the performance defects of single natural polymer materials or synthetic polymer materials, has the advantages of intercommunicated pores, uniform pore diameter, figurability, slow drug release, good bone bonding ability and good biocompatibility, and is expected to become a new osteoporosis treatment composite material.

The invention relates to a preparation method of an L-hydrogel material, which solves the technical problem of difficulty in precise regulating and controlling of osteogenesis differentiation of stemcells in the existing substrate material. The preparation method mainly comprises the following steps of (1) dissolving L-gel factors into a dimethyl sulfoxide solution to obtain an L-gel factor solution with mass volume concentration of 12 to 33 mg/ul, and putting the L-gel factor solution at the bottom of a 24-pore plate; (2) mixing the prepared L-gel factor solution into culture medium suspension liquid of mesenchymal stem cells, uniformly mixing in the 24-pore plate, standing for 30 to 60 min at a temperature of 30 to 40 DEG C, and forming a hydrogel; (3) putting the prepared hydrogel intoa mesenchymal stem cell culture medium without osteogenesis inducing factors to culture, and replacing the mesenchymal stem cell culture medium at intervals. The preparation method can be widely applied to the field of regulating and controlling the osteogenesis differentiation of three-dimensional mesenchymal stem cells by hydrogel fiber.

The invention discloses a preparation method for a micro-arc oxidation-dopamine coupling carried traditional Chinese medicine coating of a pure titanium oral implant and relates to a preparation method for a pure titanium oral implant. The preparation method disclosed by the invention aims to solve the technical problem of implantation failure as an existing titanium oral implant is slowly healed in the early implanting period and bacteria invade after implantation to lead to early infection after an implant surgery. The preparation method provided by the invention comprises the steps of: I, pre-treatment of the surface of a pure titanium test sample; II, preparation of a micro-arc oxidation electrolyte; III, ultrasonic micro-arc oxidation treatment; and IV, post-treatment. After micro-arc oxidation on the surface of a pure titanium implanting material, the material is modified by taking dopamine as a coupling agent carrying traditional Chinese medicines, so that growth and development of skeleton are promoted, material metabolism of human cells is enhanced, aging of muscles and skeleton is prevented, and the broad-spectrum antibacterial action is improved, and therefore, a novel bone implant which is better in biocompatibility and faster to heal is obtained.

The invention discloses a calcium phosphate bone cement with a hollow through structure, a preparation method and an application thereof, belongs to the technical field of calcium phosphate bone cement, wherein the calcium phosphate bone cement with a hollow through structure comprises a solid phase and a solidifying liquid, the solid phase comprises calcium phosphate powder and a fiber mixture, the fiber mixture is an absorbable fiber and a fibrin fiber, and the diameter of the absorbable fiber is 100-500mum, the length is 0.5 mm-1.5 mm, preferably, that diameter of the absorbable fiber is 200-400mum. The prepared calcium phosphate bone cement can form three-dimensional connected macropores, promote cell adhesion and proliferation, and improve the mechanical strength of the bone cement.

The invention discloses 3D printing bio-ink capable of constructing a multi-level bionic pore structure, a preparation method of the 3D printing bio-ink and a printing method. The prepared bio-ink comprises photo-curable hydrogel, a polyethylene oxide solution, an initiator, an inorganic osteogenic active component, an organic osteogenic active component and mesenchymal stem cells, and regarding a stent printed by a 3D printing technology, the porosityis 46-70%, the macropore size is 300-1000 micrometers, the microscopic size is 10-100 micrometers, and 62-90% of growth factors and cells retained in the stent can be uniformly distributed and can be proliferated and migrated through mutually communicated pores, such that the requirements of the cells in the stent for nutrition and metabolite exchange are met, and bone repair and reconstruction of the implant stent are promoted. The 3D printing bio-ink provided by the invention has good biocompatibility and good dispersibility, and can be completely degraded, such that the printed stent not only has a good bone repair effect, but also can be automatically absorbed and discharged by a human body, which does not need to be taken out by a secondary operation, and has a relatively high clinical application value.

The invention discloses application of long-chain non-coding RNA lnc-HCAR in preparation of bone repair system based on endochondral osteogenesis. The nucleotide sequence of the lnc-HCAR is SEQ ID NO.As shown in Fig. 1, the lnc-HCAR can promote the expression of Vegfa gene and Mmp13 gene, and can competitively bind to miR-15b-5p, thereby promoting hypertrophy of chondrocytes, promoting blood vessel formation in cartilage osteogenesis, and promoting Remodeling of the matrix in the cartilage osteogenesis. The bone repair system based on the long-chain non-coding RNA lnc-HCAR includes lnc-HCAR,gene delivery system, mesenchymal stem cells and porous bone scaffold materials. The invention has good application prospects in tissue engineering bone construction and bone defect repair.

The invention relates to a macroporous brushite bone cement with latent hole forming agent and preparation process, wherein the powder of the bone cement comprises 85-100% of complex calcium phosphate and 10-15% of latent gelatin as pore forming agent, the curing agent for the bone cement is 0.1-0.5 mol/L of the water solution of sodium pyrophosphate, the solid-liquid weight ratio being 2.5:1-3.0:1. Its preparation method comprises the steps of, mixing beta-tricalcium phosphate with monohydrate of monocalcium phosphate by the mol ratio of 1.1:1-1.3:1, mixing with 0-15 wt% of gelatin powder granules, so as to obtain the powder of brushite bone cement, finally charging water solution of sodium pyrophosphate with the concentration of 0.1-0.5 mol/L by the solid-liquid weight ratio of 2.5:1-3.0:1.

The invention discloses artificial bones for recovering bone defects of osteoma. The artificial bones are prepared from polylactic acid powder (PLA), hydroxyapatite powder (HA) and dimethylbiguanide powder (MET) according to mass ratios of (8 to 10) to 1 to (3 to 5). According to the artificial bones disclosed by the invention, by using dimethylbiguanide as a main effect increasing component, the aims of suppressing tumor cells and enhancing bone recovering ability are fulfilled, and the defect that the bone defects of the osteoma easily and locally recur, in particular to benign or borderline tumors such as giant cell tumors of bones is overcome; and meanwhile, because of excellent bone recovery performance, the challenge that self-recovery of massive bone defects is difficult after tumor excision is performed can be solved.

The invention discloses a bone repair material able to guide bone tissue regeneration. The material is a composition of calcined bone powder with different particle sizes and a bioabsorbable high polymer material. The bone repair material is characterized in that: the particle sizes of the calcined bone powder range from 0.1-2mm; and the bioabsorbable high polymer material is one or more of collagen, chitin, chitosan, hyaluronic acid, chondroitin sulfate, calcium alginate, fibrous protein, and elastin. The bone repair material provided in the invention has an obvious bone healing guiding effect and good biocompatibility. Also with certain hardness and strength, the bone repair material is easy to shape and has rich sources, thus solving the making problems of artificial synthetic materials in the aspects of porosity, pore traffic and pore size, etc.

The invention relates to PLA (polylactic acid)-based bone meal for the dental department and a preparation method of PLA-based bone meal. The bone meal for the dental department is prepared from poly-L-lactic acid, mineralized collagen with the mass being 50%-150% of that of poly-L-lactic acid, porous hydroxyapatite with the mass being 25%-30% of that of poly-L-lactic acid and lecithin with the mass being 5%-20% of that of poly-L-lactic acid. The preparation method comprises the steps as follows: preparation of a poly-L-lactic acid solution, blending, mold filling, freeze-drying, analysis, crushing and screening. The PLA-based bone meal material for the dental department has good biocompatibility and an excellent hydrophilic effect, can improve the bone repair effect, also has a good developing effect and is convenient to apply clinically.

The invention relates to a bone repair stent material and a preparation method thereof, and the bone repair stent material can be used to overcome the defects of difficult obtainment of raw materials and ethical problems in the existing practice of taking a small bovine bone as the bone repair stent material. The bone repair stent material contains hydroxyapatite, the material is in a porous interpenetrating network structure, the porosity is 30%-85%, the macropore diameter is 100-800 microns, and the micropore diameter is 10-60 microns. The stent material can be widely used in repair treatment of bone defects and bone loss caused by diseases, traffic accidents, sports injuries and the like.

The invention relates to the technical field of biomedical materials, which relates to a 3D printing material, in particular to a 3D printing multilayer micro-nano structure controllable porous bioactive ceramic material. The bioactive ceramic scaffold of the invention is a model with a certain macropore through parametric modeling design, and 3D printing technology is used to shape a certain ratio of calcium phosphate raw materials into the designed model. The porous calcium phosphate scaffolds were fabricated by sintering the calcium phosphate scaffolds, and then the micro-nano-structure ofthe scaffolds was changed by solution recrystallization. In this invention, by adjusting the ratio of hydroxyapatite (HA) to tricalcium phosphate (TCP) in the raw material, the primary macropore structure of the scaffold was designed, and the secondary micro-nano morphology and structure of the scaffold were controlled by controlling the environmental parameters of the solution recrystallization reaction medium, such as temperature, time, pH value of the medium and so on. The scaffolds for bone tissue engineering could be customized according to different patients.

The invention belongs to the technical field of biological ceramic materials and additive manufacturing, and particularly discloses a photocuring 3D printing ceramic composite material and a preparation method and application thereof. The light-cured 3D printing ceramic composite material is prepared from the following raw materials: basic ceramic powder, light-cured resin, an inorganic toughening agent and an auxiliary agent, the light-cured resin comprises first light-cured resin and second light-cured resin, the refractive index of the first light-cured resin is 1.42-1.52, and the refractive index of the second light-cured resin is 1.60-1.70; the volume ratio of the first light-cured resin to the second light-cured resin is (9: 1)-(2: 8). According to the preparation method disclosed by the invention, the biological ceramic, especially a fine complex pore structure with highly communicated interior, can be prepared through photocuring, the toughness of the biological ceramic implant for craniomaxillofacial is remarkably improved, the fracture toughness can reach 0.769-0.869 MPa.m < 1/2 >, and the bending strength is 6.18-7.84 MPa.

The invention relates to an electrified repair film material controllable in surface potential and solves the technical problem that existing materials are not adjustable in surface potential, cannotbe adapted to electrophysiological microenvironment of each tissue defective position and limited in material repair effect. The electrified repair film material is a thin-film-shaped material prepared from ferroelectric high-molecular polymer and an organic solvent. The invention further provides a preparation method of the electrified repair film material. The electrified repair film material can be widely applied in the technical field of surgical implanting of bionic electrified microenvironment repair materials.

The invention relates to a biological composite artificial bone and its preparation process. The artificial bone is prepared by poly lactic acid, hydroxyapatite(or calcium phosphate) and OPG protein with weight ratio of 180-250 : 100-160 : 0.6-1. The invention mixes poly lactic acid, hydroxyapatite(or calcium phosphate) and OPG protein only once by emulsion cross blend method without changing their chemical structure. The adopted ethyl acetate and absolute ethyl alcohol have no harm to the human body, and the hydroxyapatite(or calcium phosphate) bone conduction function and the osteoclast genesis and activation of the OPG protein are kept. It can also initiate osteoclast apoptosis. The compound product is a biological compound artificial bone for treating bone defection and osteoporosis in clinical practice.

The invention discloses a tissue engineering hydrogel scaffold for promoting cell migration. The tissue engineering hydrogel scaffold is prepared from 1-2 parts of polyethylene glycol (glycol) diacrylate, 8-32 parts of a photoinitiator, 0.15-1.5 parts of polyphosphate and 1.5-3 parts of sodium alginate. The invention further discloses a preparation method of the tissue engineering hydrogel scaffold for promoting cell migration, 3D printing slurry for preparing the tissue engineering hydrogel scaffold for promoting cell migration and a preparation method of the 3D printing slurry. The tissue engineering hydrogel scaffold is good in safety and excellent in mechanical property, meanwhile, the extracellular ATP content can be increased, the physiological activity of cells is promoted, and cell migration is facilitated.