55 results about "Giant cell" patented technology

The invention provides a kit for genotyping VZV (Varicella-Zoster Viruses). The kit is characterized by comprising a nucleotide sequence shown as the Table 3 in the specification, and specific primers and specific probes corresponding to clade1-5 type VZV of chemical structures. The kit has the function of detecting various kinds of VZV DNA (Deoxyribonucleic Acid); the detection sensitivity is 10<2> copies/reaction; no cross reaction with human genome, herpes simplex viruses type I/type II, cytomegaloviruses and EB viruses exists; and the kit is applicable to the virus gene diagnosis of clinical VZV infected persons, and can also be used for the epidemiology survey of different Clade types of VZV.

Disclosed is a nucleic acid comprising a nucleotide sequence encoding (i) two or more reporters comprising a first reporter and a second reporter that is different from the first reporter; and (ii) one or more ribosomal skip sequences, wherein a ribosomal skip sequence is positioned between the first and second reporters, wherein the first and second reporters are stoichiometrically co-expressed from the nucleotide sequence and the nucleic acid does not comprise a cytomegalovirus-immediate early (CMV-IE) promoter. Also disclosed are methods of screening test compounds for ability to modulate a biological activity of interest using the nucleic acid, as well as related recombinant expression vectors, host cells, and populations of cells.

The invention relates to an allogenic tumor cell line which is modified by GM-CSF gene, and the application in the preparation of tumor vaccine; the invention discloses an allogenic tumor cell line which is modified by the GM-CSF gene, and the application in the preparation of the tumor vaccine.

The invention discloses a method for manufacturing water convolvulus root-knot nematode giant cell paraffin sections. According to the method, steps of staining, cross-sectioning angle, de-waxing andthe like are improved on the basis of conventional paraffin section technologies. The Ehrlich's hematoxylin staining solution is used for staining of plant tissues, and traditional dewaxing before staining is changed into mass staining before dewaxing. Section angle adopts cross-sectioning, and section thickness is 5-10 microns. According to the invention, dewaxing mode is also improved, and a TO-type biological slice-making transparent reagent is used to replace xylene for dewaxing. By the manufacturing method of water convolvulus root-knot nematode giant cell paraffin sections, staining effect of water convolvulus root-knot nematode giant cell paraffin sections is obviously enhanced, work efficiency is raised, and the effect of water convolvulus root-knot nematode giant cell paraffin sections is guaranteed.

The sample pad and the binding pad, which is the conjugate coated with the anti U chain monoclonal antibody and colloidal gold, are adhered to the one end with PVC back covered in sequence. The cellulose nitrate film coated with the specificity surface film antigen of giant cells virus and the anti rat IgG of sheep (or rabbit) is adhered to the middle of the reagent. The water uptake pad is adhered to another end of the reagent. The reagent features high specificity and sensivitiy, simple and fast. The reagent is applicable to the detection in site within 30 minutes for the full testing procedure. Operation personnel can complete the operation by reading the menu and without need of professional training.

The invention provides a technology of isolating and culturing umbilical cord mesenchymal stem cells from umbilical cords of volunteers for reproductive health, relates to the fields of basic medicine and reproductive health medical science. Stem cells are isolated and purified from umbilical cord tissues of a volunteer, and cultured for multiplication in a particular culture system. When the total stem cells reach a certain number, the stem cells are used for maintenance of the reproductive system through intravenous infusion. The mesenchymal stem cells can be used for maintenance of reproductive system and treatment of dysfunction, and have the advantages that the source is rich, the cells are stored in kind and can be recovered or cultured and amplified when necessary, the in vitro amplification ability is high, tissue typing is not needed for transplantation, and the graft-versus-host disease (GVHD) and cytomegalovirus (CMV) infection do not occur. The umbilical cord mesenchymal stem cell technology for health care or treatment has no toxic side effects and adverse reactions.

Provided are detection and assay of CMV that can rapidly amplify and detect the beta 2.7 gene originating from CMV at a certain temperature through a one-step operation. The mRNA of beta 2.7 gene originating from CMV is amplified by utilizing the combination of specifically amplifiable oligonucleotide primers and the RNA amplification step comprising these combinations of oligonucleotide primers is measured and detected by using an oligonucleotide probe that is marked with an intercalated fluorescent dye, to settle above topic.

The invention provides a novel and highly-sensitive detection method for human cytomegalovirus (HCMV). The method represents a nested quantitative PCR (polymerase chain reaction) technique which can detect both free HCMV and HCMV that infects a cell, and is characterized in that nucleic acid is extracted from human peripheral blood mononuclear cells (PBMCs), and a first round of PCR amplification is carried out through specific outer upstream and downstream primers; with the products of the first round of PCR amplification serving as a template, a second round of quantitative PCR amplification is carried out through specific inner upstream and downstream primers. By adopting the detection method provided by the invention, the HCMV detection sensitivity can reach 8 copies per microliter. Compared with the ELISA (enzyme-linked immunoabsorbent assay) method, compared with the ELISA method, the method adopting the PCR method for the detection of the latent HCMV infection has the advantages of rapidness, simplicity, high sensitivity, strong specificity, good stability and the like, and is a clinical method for early detection and diagnosis.

The invention discloses a new establishment method and application of a cell strain for stably human cytomegalovirus (HCMV) pp65 protein in nucleus. A nucleus localization signal (pp65-NLS) is added to the 3' end of the HCMV pp65 gene, so that the endonuclear expressed pp65 gene transfected by the gene has the same cellular localization as the host-cell-infected HCMV; and the pp65-NLS is integrated into the K562 cell under the mediation of slow virus, thereby establishing the K562 cell strain for stably expressing 9965-NLS. The cell strain can be used as a positive reference substance of an HCMV 9965 antigenemia detection kit, thereby implementing the reliable application of the kit in clinical diagnosis.

The invention discloses a broad-spectrum chemokine receptor antagonistic polypeptide and an application thereof. The amino acid sequence of the broad-spectrum chemokine receptor antagonistic polypeptide is VLNAHCALH. The broad-spectrum chemokine receptor antagonistic polypeptide in the invention can be well combined with US28 receptors on cell membranes, lowers the increased calcium ion concentration caused by combination of physiological chemokines and receptors, has an effect of blocking receptor signal transduction, inhibits human cytomegalovirus AD169 and HIV-1 induced negativecolony fromformation in vitro, restrains virus from reproducing in cells, reduces death of cells infected by virus, and can be used as an antagonist for broad spectrum inflammation and virus chemokine and applied in relative work for researching virus prevention and treatment.

The invention discloses an extractive of a compound formula of ophiopogon decoction and caper euphorbia seed and reed stem soup. The extractive is thick paste which is obtained by treating a compoundformula of ophiopogon decoction and caper euphorbia seed and reed stem soup by a series of steps of water extraction, alcohol precipitation and extraction of cyclohexane and ethylacetate. The invention discloses a preparation method of the extractive and also discloses the application of the extractive in preparing a medicament for inhibiting H460 cell proliferation. The extractive of a compound formula of ophiopogon decoction and caper euphorbia seed and reed stem soup is characterized in that the extraction of the ethylacetate can obviously inhibit the growth of human lung giant cell carcinoma H460 and has no obvious influences on human normal cells, and the extraction has the effect of cell mediated cytotoxicity and can further separate and screen components and monomeric compounds forinhibiting the proliferation of the human lung giant cell carcinoma H460.

Described herein are CSF1R inhibitors for use in methods of treating cancers and other tumors related to the decreased proliferation, the depletion, or the repolarization of tumor-associated macrophages (TAMs) and treatment of associated disorders, including tenosynovial giant cell tumor (TGCT) and diffuse-type tenosynovial giant cell tumor (DTGCT).

The invention provides a recombinant replication-defective adenovirus for expressing an hTERT (human telomerase reverse transcriptase) gene. A target gene hTERT is obtained by virtue of enzyme digestion from a plasmid pIRES2-EGFP(enhanced green fluorescent protein)-hTERT, the target gene hTERT is inserted to downstream of a cytomegalovirus promoter in a pSG218 shuttle vector to obtain a recombinant shuttle vector with the hTERT gene; and the recombinant shuttle vector and a virus framework vector pPE3-F11B are co-transferred to a human embryo kidney 293 cell to obtain replication-defective adenovirus with hTERT. The recombinant adenovirus infects a human multiple myeloma cell, so that the cell excessively expresses the hTERT gene, and therefore, drug resistance of the cell on pharmorubicin is strengthened. The recombinant replication-defective adenovirus disclosed by the invention is mainly used for establishing a multiple myeloma cell strain model for excessively expressing hTERT gene, and further researching a mechanism that the hTERT gene participates in drug resistance of the multiple myeloma cell based on the model.

The invention discloses a method for identifying resistance of a plant to meloidogyne. The method comprises the steps of 1, grafting meloidogyne on a plant, and conducting cultivation; 2, after the step 1 finishes, taking plant root tissue, and observing the growth state of meloidogyne in the plant root tissue; 3, after the step 1 finishes, taking the plant root tissue, and calculating the number of meloidogyne in the plant root tissue; judging the resistance of the plant to meloidogyne according to the result. According to the method for identifying the resistance of the plant to meloidogyne, toluidine blue is applied to dyeing of a root-knot paraffin slice, an optimal dyeing time is found, and the growth state of the nematodes and giant cells can be clearly observed; moreover, the dyeing time is fast, and the effect is good. Aiming at the statistics on the number of the nematodes, a grinding method and a screening method are combined, an optimal grinding time is found, and convenience is brought to the statistics on the number of the nematodes. The two methods can combined into application, and the resistance of the plant to the statistics on the number of the nematodes can be expressed from two aspects of the growth state and the nematodes and the number of the nematodes.

The invention provides a tumor vaccine and a preparation method thereof. The preparation method comprises: extracting mRNA from human peripheral blood monouclear cells, synthesizing cDNA in vitro, employing polymerase chain reaction technology to clone granulocyte macrophage colony-stimulating factor (GM-CSF) cDNA containing a signal peptide, recombining with sendai virus vector, so as to construct a recombinant sendai virus vector GM-CSF-SV containing granulocyte macrophage colony-stimulating factor cDNA; and employing a packaging cell 293T with relatively high safety to package the recombinant sendai virus vector GM-CSF-SV containing GM-CSF gene, so as to generate he recombinant sendai virus vector which is high in titer and does not have duplication capability; and infecting human lung cancer cell strain A549, so as to establish a gene engineering human lung cancer cell with GM-CSF expression activity, and employing <60>Co to irradiate and inactivate a tumor vaccine and performing heat shock processing, and preparing the GM-CSF gene modified human lung cancer cell tumor vaccine after different irradiation amounts are explored.

Hematopoietic stem cells are extremely difficult to maintain or expand in vitro. Two observations in traditional long-term bone marrow cultures strongly suggest that macrophages may be at the root of the problem: First, micromolar concentrations of hydrocortisone improve the longevity of long-term bone marrow cultures and hydrocortisone is known as a potent inhibitor of macrophage production of pro-inflammatory cytokines, chemokines, enzymes, nitrogen oxide and reactive oxygen species and redirects macrophages to the anti-inflammatory differentiation pathway; Second, the decline of hematopoiesis in long-term bone marrow cultures coincides with the development of large numbers of adherent and non-adherent macrophages including foreign body giant cells. These adherent macrophages and foreign body giant cells exhibit well-spread morphology, contain numerous lysosomes and phagolysosomes in the cytoplasm and are metabolically active. We hypothesize that hydrocortisone fails to suppress all aspects of macrophage pro-inflammatory activation/differentiation, resulting in the production of inhibitors or toxins of hematopoiesis. Macrophage adhesion in cell culture depends on serum proteins pre-adsorbed to the tissue-culture-treated polystyrene (TC-PS), which adsorbs proteins via mostly hydrophilic interactions. TC-PS is used in almost all tissue culture devices currently available. Cellular adhesion provides a strong stimulus for metabolic, mitotic and certain gene activities. Therefore, we seek to reduce macrophage adhesion and activation by culturing bone marrow cells in tissue culture devices composed of or covered with polymers with very different protein-binding characteristics than TC-PS such as polyethylene (PE) and other polyolefins, the latter bind proteins via exclusively hydrophobic interactions. As a result, polyolefins bind different proteins and in lower quantities than TC-PS. Furthermore, PE does not contain additional chemical features like the phenolic rings of polystyrene that might contribute to protein binding and macrophage adhesion/activation. Using these new culture devices, we developed a drastically different long-term bone marrow culture, the “Low Macrophage-Adhesion/Activation” (LoMAC) bone marrow culture. In LoMAC bone marrow culture, hematopoiesis continues for months to over a year and hematopoietic stem cells are amplified gradually. In stark contrast to traditional long-term bone marrow cultures, de novo erythropoiesis and megakaryocytopoiesis proceed robustly in the LoMAC bone marrow culture and B-lymphocyte and natural killer cell progenitors can be continuously derived. Thus, these new culture devices and the associated LoMAC culture method offer a new way to study hematopoiesis in vitro and provide a more robust platform for the expansion of hematopoietic stem cells and progenitors ex vivo.

The invention relates to a method for positioning an animal SLD nucleus. The invention provides a method for determining the anatomo-functional delimitation of a sublaterodorsal tegmental nucleus (SLD) of a tested object. The method comprises the following steps that 1) a reverse transport virus vector is stereodirectionally injected into an SLD downstream nucleus abdominal side gigantocellular reticular nucleus (GiV), and under the action of a neuron alternative promoter, the reverse transport virus vector can specifically express a first marker in an upstream efferent neuron; 2) REM sleep deprivation and rebound experiments are conducted on a subject, and a functional active neuron is determined through a second marker, wherein the second marker is different from the first marker; and 3) the anatomo-functional delimitation of the SLD nucleus is determined according to the co-marker fusion range of the first marker and the second marker. According to the method, through ingenious combined application of nerve fiber projection and sleep physiology research methods, the anatomo-functional delimitation of the SLD nucleus can be determined visually and accurately.

Provided herein are, inter alia, methods and compositions including T cells expressing (i) a recombinant CAR protein which includes a peptide binding site and is capable of specifically binding cancer-specific antigens and (ii) a T cell receptor specific for a viral antigen (e.g., a CMV pp65 protein). The engineered T cells provided herein may be used in combination with a viral vaccine (e.g. cytomegalovirus (CMV) Triplex Vaccine) to treat a variety of cancers. The methods described herein also permit in viva expansion of CMV-specific CAR T cells, instead of or in addition to ex vivo expansion, avoiding excessive T cell exhaustion that results in some cases from ex vivo manufacturing.