61results about How to "Cytotoxic" patented technology

The invention relates to a Bacillus sp.N11-8 and an active component thereof having antitumor action, belonging to the technical field of marine microorganism medicine. The Bacillus sp.N11-8 is obtained by isolated culture in Antarctic Ocean water, and was collected at China Center for Type Culture Collection (Wuhan) on November 19, 2012; and the collection number is CCTCC NO:M2012459. The strain can stably express the active component having antitumor action. The active component has favorable acid / alkali stability and heat stability, and has bigger cytotoxic effect on multiple tumor cells and smaller cytotoxic effect on normal cells of humans. Thus, the Bacillus sp.N11-8 and the active component thereof have favorable research and application values.

The invention relates to a specific blocking agent of a Toll-like receptor 9 (TLR9). The blocking agent is a nonmethylated CpG nucleotide sequence segment, the core structure of which is a CGCG nucleotide sequence, and the length of the nonmethylated CpG nucleotide sequence segment is 12-30 nucleotides. The blocking agent can be specifically combined with the TLR9, can block the combination between other irritant aglucons and the TLR9 and inhibit the proinflammatory effect mediated by the receptor, and can be used for the adjuvant treatment of inflammatory resistance and allergy resistance and the preparation of an experiment reagent of immune inflammation.

The invention provides a skin moisturizer for treating a skin disease and a preparation method of the skin moisturizer. The skin moisturizer is mainly prepared from the following raw materials in parts by weight: 10-20 parts of alginate oligosaccharide, 0.5-5 parts of a solubilizer, 0.5-1 part of sodium chloride, 5-10 parts of a dispersing agent, 5-20 parts of a tackifier, 2-3 parts of a preservative and 2-3 parts of an antioxidant, wherein the solvent is injection water; and the preparation method comprises the following steps: dissolving 0.2-0.3 part of sodium chloride into residual injection water; adding the solubilizer, the tackifier, the preservative, the dispersing agent and the antioxidant, stirring evenly, and adjusting the pH value to be 3.5-6.5; dissolving the alginate oligosaccharide into the residual injection water, adding residual sodium chloride, stirring and mixing with the substances obtained from the steps; and packing and sterilizing to obtain the skin moisturizer. The skin moisturizer provided by the embodiment has the effects of good treatment effect, mild medicine property and no side effect.

The invention discloses a dry artificial biological heart valve containing polypeptide for promoting endothelialization and a preparation method of the dry artificial biological heart valve. The method comprises the following steps that cross-linking is performed on amino groups and carboxyl groups of an animal pericardium, and endothelialization treatment, hydrogel polymerization treatment and dewatering and drying treatment are performed on the animal pericardium, wherein the endothelialization treatment involves that the polypeptide for promoting the endothelialization is adopted to treat the animal pericardium. The preparation method has the advantages that the prepared dry biological heart valve has good water immersion flattening performance, and can be quickly immersed in water andflattened at a higher temperature and higher humidity, thereby reducing residues of glutaraldehyde and the like, and reducing the problems of calcification and toxicity which are caused by the glutaraldehyde and the like, therefore the preoperative installation of a valve system can be simplified, and the additional risks of surgeries are reduced; the polypeptide capable of promoting the endothelialization is also added in the preparation process, so that the prepared biological valve has the property of promoting the endothelialization.

The invention provides an anti-CD123 nano antibody and application thereof. The nano antibody comprises a heavy chain variable region, wherein the heavy chain variable region comprises CDR1 as shown in SEQ ID NO: 1, CDR2 as shown in SEQ ID NO: 2 and CDR3 as shown in SEQ ID NO: 3. The antibody screened from the camel VHH immune library has CDR regions shown as SEQ ID NO: 1-3, the nano antibody formed by combining the antibody with different framework regions FR has strong affinity with CD123, so that the constructed chimeric antigen receptor and chimeric antigen receptor immune cells have remarkable cytotoxicity on CD123 positive cells, and have wide application prospects in the field of tumor treatment.

The invention discloses a dry artificial biological heart valve containing a growth factor for promoting endothelialization and a preparation method of the dry artificial biological heart valve. The method comprises the following steps that cross-linking is performed on amino groups and carboxyl groups of an animal pericardium, and endothelialization treatment, hydrogel polymerization treatment and dewatering and drying treatment are performed on the animal pericardium, wherein the endothelialization treatment involves that the growth factor for promoting the endothelialization is adopted to treat the animal pericardium. The preparation method has the advantages that the prepared dry biological heart valve has good water immersion flattening performance, and can be quickly immersed in water and flattened at a higher temperature and higher humidity, thereby reducing residues of glutaraldehyde and the like, and reducing the problems of calcification and toxicity which are caused by the glutaraldehyde and the like, therefore the preoperative installation of a valve system can be simplified, and the additional risks of surgeries are reduced; the growth factor capable of promoting the endothelialization is also added in the preparation process, so that the prepared biological valve has the property of promoting the endothelialization.

The invention discloses a production method and a donkey-hide gelatin of a Chinese herbal donkey-hide gelatin material, which belong to the deep development of rare herbal donkey-hide gelatins, the technology for replacing materials of the rare herbal donkey-hide gelatins and relevant products of the rare herbal donkey-hide gelatins in the sustainable utilization field of Chinese herbal resources. According to the method, any one or multiple or all of litsea cubeba oil, lucid ganoderma, mushroom, hericium erinaceus, trametes robiniophila Murr, poria cocos, polyporus umbellatus, rainbow conk, marasmius androsaceus, armillaria mellea, false armillaria mellea, cordyceps sinensis, cordyceps militaris, tremella fuciformis, flammulina velutipes, edible fungus, grifola frondosa, gloeostereum incarnatum, schizophyllum commune, fomitopsis pinicola, cordyceps cephalosporium, paecilomyces hepialid, cordyceps gunnii penicillium, litsea cubeba, bamboo fungus, phellinus igniarius and edible fungus are added into water for peel soaking, peel removing, peel cutting, peel washing, complete peel removing and gelatin juice extraction during the production process of the Chinese herbal gelatin. The method has the most obvious characteristic that the problems that the donkey-hide gelatin material is deficient, the putrefactive odor is dispersive by the donkey-hide gelatin material during the production of the donkey-hide gelatin and the finished donkey-hide gelatin product is poor in taste are solved.

The invention discloses a tumor-targeted nanometer compound, a preparing method and application of the compound to acoustic-power-mediated precise tumor treatment. The nanometer compound comprises compound nanoparticles wrapped by hyaluronic acid. A preparing method of the compound nanoparticles includes the steps of converting part of amino groups on the surface of a dendrimer PAMAM into carboxylgroups through succinic anhydride, and compounding the dendrimer PAMAM with a sensitizer (indocyanine green), an anti-tumor medicine (doxorubicin) and hyaluronic acid. The nanometer compound has a good acoustic power anti-tumor effect, the sensitizer and the anti-tumor medicine have a good synergistic effect in treatment, and the nanometer compound is suitable for noninvasive / minimally invasive tumor treatment.

This invention relates to a cycloartane triterpenoid compound and its anti-tumor applications. The compound, with a general chemical formula shown in formula I, has in vitro anti-tumor activity. The compound and its derivatives can be used to manufacture anti-tumor drugs.

The invention discloses a method for synthesizing benzo[c]pyridine[4,3,2-mn]acridine-8-one. The method comprises the following steps of: performing intramolecular tandem cyclization reaction on 2-[3-(2-amidophenyl)-2-propynylamino]quinone with a 1,5-eneyne structure under the action of a metal catalyst, and purifying to obtain the benzo[c]pyridine[4,3,2-mn]acridine-8-one, wherein R refers to hydroxyl or methoxy; R1 and R2 refer to halogen atoms or alkyl with 1 to 6 carbon atoms; the intramolecular tandem cyclization reaction is homogeneous metal catalysis reaction, and the metal catalyst is gold salt or univalent gold coordination complex; the using amount of the catalyst is equivalent to 0.01 to 0.5 of the quinone raw material; and after the intramolecular tandem cyclization reaction is finished, purification, namely quick column chromatography, is carried out and the benzo[c]pyridine[4,3,2-mn]acridine-8-one is obtained. The raw material is simple and readily available, the reaction conditions are mild, and the chemical regioselectivity is high.

The invention discloses application of an n-butyl alcohol extract of a combination of ophiopogon decoction and thousand gold pieces phragmites decoction in preparing medicaments for inhibiting A549 cell proliferation. In the invention, the n-butyl alcohol extract of the combination of the ophiopogon decoction and the thousand gold pieces phragmites decoction can remarkably inhibit the growth of lung adenocarcinoma cell A549, and has no obvious influence on normal cells of a human body. Because the extract part has a cytotoxic effect, components and monomer compound used for inhibiting the lung adenocarcinoma cell A549 can be further separated and selected.

The invention relates to donkey-hide gelatin, a preparation method and associated equipment, which belong to the deep development of rare herbal donkey-hide gelatins, the technology for replacing materials of the rare herbal donkey-hide gelatins and relevant products of the rare herbal donkey-hide gelatins in the sustainable utilization field of Chinese herbal resources. According to the method, any one or multiple or all of litsea cubeba oil, lucid ganoderma, mushroom, hericium erinaceus, trametes robiniophila Murr, poria cocos, polyporus umbellatus, rainbow conk, marasmius androsaceus, armillaria mellea, false armillaria mellea, cordyceps sinensis, cordyceps militaris, tremella fuciformis, flammulina velutipes, edible fungus, grifola frondosa, gloeostereum incarnatum, schizophyllum commune, fomitopsis pinicola, cordyceps cephalosporium, paecilomyces hepialid, cordyceps gunnii penicillium, litsea cubeba, bamboo fungus, phellinus igniarius and edible fungus are added into water for peel soaking, peel removing, peel cutting, peel washing, complete peel removing and gelatin juice extraction during the production process of the Chinese herbal gelatin. The method has the most obvious characteristic that the problems that the donkey-hide gelatin material is deficient, the putrefactive odor is dispersive by the donkey-hide gelatin material during the production of the donkey-hide gelatin and the finished donkey-hide gelatin product is poor in taste are solved.

The invention belongs to the field of medical technology and relates to total alkaloids which are extracted and separated from leaves of Buxusmicrophyllavar.koreanaNakai and have in-vitro antitumor activity, and a preparation method of the total alkaloids, as well as five buxus alkaloid compounds separated from the leaves of Buxusmicrophyllavar.koreanaNakai, and a preparation method of the five buxus alkaloid compounds. raw materials of the extract are easily and massively obtained, the obtained monomeric compounds are novel in structure and has in-vitro antitumor activity, the method for extracting and separating the total alkaloids and active monomeric compounds in the total alkaloids is simple, pharmacological and clinical research on the total alkaloids and active monomeric compounds are facilitated and conditions for developing novel antitumor drug with good curative effect and fewer toxic and side effects are created.

The invention relates to tea and a preparation method of the tea, and in particular relates to pseudo-ginseng health-preserving tea and a preparation method of the pseudo-ginseng health-preserving tea. The pseudo-ginseng health-preserving tea is prepared from the following raw materials in parts by weight: 3-5 parts of pseudo-ginseng, 3-5 parts of bark of eucommia, 20-30 parts of common selfheal fruit-spike, 10-20 parts of tall gastrodia tuber, 10-15 parts of glossy ganoderma and 40-50 parts of cyperus rotunus. The preparation method comprises the following steps: completely cleaning the fresh raw materials, preparing into particles, baking or drying in the sun, and then compounding according to the weight parts. The pseudo-ginseng health-preserving tea disclosed by the invention has the effects of dissipating blood stasis and stopping bleeding, dispersing swelling and settling pain, clearing away the liver fire, removing stasis and dispersing swelling, clearing heat and removing toxicity, expelling phlegm and arresting cough, treating acute infectious icteric hepatitis and bacterial dysentery, and the like.

The invention discloses a polysiloxane-based polyurethane / lignin elastomer as well as a preparation method and application thereof, and belongs to the technical field of preparation of polyurethane / polysiloxane elastomers. Thepreparation method comprises the following steps: (1) mixing an isocyanate, a catalyst, a polycarbonate diol and an organic solvent to obtain a prepolymer mixed solution; then adding polysiloxane and ligninfor continuous mixing reaction, carrying out cooling treatmentafter the reaction is finished to obtain a polysiloxane-based polyurethane / lignin solution; and (2) carrying out molding treatment on the polysiloxane-based polyurethane / lignin solution to obtain the polysiloxane-based polyurethane / lignin elastomer. According to the invention, the characteristics that lignin is abundant in resource, easily available and renewableare utilized, and a cross-linked structure or filler effect is formed under the action of covalent bonds, so that the polyurethane elastomerhas the characteristics of hydrophobicity, high mechanical strength and cytotoxicity resistance.

The invention discloses a polymer with targeted nuclear magnetic resonance imaging and fluorescence imaging functions, a preparation method and an application, and belongs to the technical field of nuclear magnetic resonance imaging. The preparation method comprises the following steps: S1, preparing a preliminary product; S2, preparing a copolymer; S3, preparing a block copolymer; S4, preparing an end-modified block copolymer; and S5, preparing the fluorescent targeted nuclear magnetic resonance imaging polymer with high relaxation rate. In the step S1, an eluent used when an obtained productis separated by a silica gel column is a mixed solution of ethyl acetate and normal hexane, and the volume ratio of the ethyl acetate to the normal hexane is 1: 3. The polymer prepared by the invention has the functions of nuclear magnetic resonance imaging and fluorescence imaging at the same time, and has a good application prospect in clinical diagnosis and guided surgical resection; the polymer has low toxicity, excellent biocompatibility and low cytotoxicity, and is a safe material; and the structure is stable, the relaxation rate is high, and high clinical and market application potential is achieved.

The invention discloses an anticancer and antitumor traditional Chinese medicine formula including dried toads, paris polyphylla, sculellaria barbata, herba rabdosiae, subprostrate sophora, flos euphorbiae lantu, musk, ginseng, astragalus membranaceus, frankincense, myrrh and salviae miltiorrhizae according to the weight ratio as follows: 0.3-0.8 parts of the dried toads, 0.8-1.5 parts of the paris polyphylla, 3-7 parts of the sculellaria barbata, 3-7 parts of the herba rabdosiae, 1-3 parts of the subprostrate sophora, 1-1.5 parts of the flos euphorbiae lantu, 0.03-0.06 parts of the musk, 0.5-2 parts of the ginseng, 2-4 parts of the astragalus membranaceus, 2-4 parts of the frankincense, 2-4 parts of the myrrh and 3-7 parts of the salviae miltiorrhizae. The traditional Chinese medicine isconvenient to take and can treat both exterior and interior, has significant curative effect and low toxic and side effect, is low in cost, is mainly used for treating breast cancer, lung cancer, rectal cancer and cancers and tumors of digestion system, and also has potential inhibition effect on other types of cancers and tumors.

The invention discloses an anti-CD20 single chain antibody having a heavy chain variable region amino acid sequence as shown in SEQ ID No:2 and a light chain variable region amino acid sequence as shown in SEQ ID No:3. The invention further discloses a fully humanized anti-CD20 recombinant antibody, which comprises the single chain antibody and a human antibody constant region Fc fragment, the amino acid sequence of the recombinant antibody is as shown in SEQ ID No:17. The invention further discloses an eukaryotic expression vector. The eukaryotic expression vector is obtained by inserting thesequence of the anti-CD20 singlechain antibody into a universal expression vector sp-Fc / pcDNA3.1, and the nucleic acid sequence of the universal expression vector is as shown in SEQ ID No:18. The invention further discloses a recombinant expression vector.The eukaryotic expression vector is used as a template, an amplification primer is used for amplifyinga fragment sp-scFv966-Fc, and the obtained amplified fragment is inserted into a pMH3 vector.

The invention discloses a composite film with a titanium dioxide layer and gold nanoparticles on the surface. The composite film sequentially comprises a base film, a titanium dioxide layer and nanogold particles, wherein the nanogold particles are dispersed on the titanium dioxide layer, the diameter of the nanogold particles is 0.5-200 nm, and the mass of the nanogold particles on each square meter of the composite film is 0.03-0.05 g. The invention also discloses a preparation method and application of the composite film. The nanogold particles are deposited on the titanium dioxide layer, so that the visible light absorption capacity of the titanium dioxide modified layer can be improved, and the problems of weak visible light absorption and limited application range of a composite filmtaking titanium dioxide as a main component are solved. The number of free radicals is increased, chemical damage is enhanced, and the antibacterial effect is further improved. The nanogold particleson the surface of the composite film are small in diameter and small in dosage, the efficient antibacterial effect can be achieved by depositing the nanogold particles with the extremely-low amount,and the cost can be reduced. In addition, the composite film can also be used for decomposing dyes.

An antineoplastic and antioxidizing active component of bugleweed for preparing the antioxidizing and antineoplastic medicines is composed of more components respectively extracted by alcohol, petroether, ethyl acetate, n-butanol, n-hexane, dichloromethane, or their mixture. Its preparing process is also disclosed.

The invention provides application of a DNA tetrahedral nanostructure as a carrier of a medicine for treating inner ear diseases. The DNA tetrahedral nanostructure reaches inner ear functional cells through round window injection. According to the invention, a mouse round window administration technology successfully proves that the DNA tetrahedral nanostructure can enter inner ear functional cells. Therefore, the enrichment of the DNA tetrahedral nanostructure in the hearing hair cell part of the inner ear is realized for the first time, and a new way is provided for conveying medicines for treating inner ear diseases. Meanwhile, nucleic acid molecules are natural and have the advantages of low toxicity, excellent biocompatibility and the like, and the synthesis price of framework nucleic acid is low (the injection cost of each time is about several yuan), so that the DNA tetrahedral nanostructure provided by the invention is an ideal drug carrier for treating inner ear diseases.

The invention provides a nutritious food health tea granule for preventing tumor, which belongs to the field of nutritious foods and aims to realize good effect and quick effectiveness. The nutritious food health tea granule is made from chrysanthemum, garlic, grape seed, and on the like. The nutritious food health tea granule has the advantages of significant effect in preventing tumor, precise function, good effect and quick effectiveness.

The invention relates to a specific blocking agent of a Toll-like receptor 9 (TLR9). The blocking agent is a nonmethylated CpG nucleotide sequence segment, the core structure of which is a CGCG nucleotide sequence, and the length of the nonmethylated CpG nucleotide sequence segment is 12-30 nucleotides. The blocking agent can be specifically combined with the TLR9, can block the combination between other irritant aglucons and the TLR9 and inhibit the proinflammatory effect mediated by the receptor, and can be used for the adjuvant treatment of inflammatory resistance and allergy resistance and the preparation of an experiment reagent of immune inflammation.

The invention belongs to the technical field of functional medical materials, and particularly relates to a photo-stimulated active oxygen responsive hollow mesoporous silica drug-loaded particle and a preparation method and application thereof. The photo-stimulated active oxygen responsive hollow mesoporous silica drug-loaded particle comprises a hollow mesoporous silica carrier and a covalent binding prodrug loaded on the surface of the hollow mesoporous silica carrier, and drugs are loaded in a hollow inner cavity and mesopores of the hollow mesoporous silica carrier. According to the invention, covalent binding prodrugs are introduced on the surface of hollow mesoporous silica, so that loading of various drugs is realized; the covalent binding prodrug not only can prevent the drug from leaking in systemic circulation, but also can be used as a goalkeeper of the hollow mesoporous silicon dioxide carrier to control the release of the drug in the hollow mesoporous and enhance the inhibition on tumor growth.

The invention discloses a traditional Chinese medicine bamboo shoot effective position and preparing method, which is characterized by the following: the effective position of bamboo shoot extract is selected from one and combination of skellysolve B, acetic ester and butyl alcohol, wherein the position of acetic ester and butyl alcohol possesses obvious external ultra-oxygen negative ion free-base removing ability, Fe3+ reducing ability and hyper-oxidizing proof activity of mouse liver particle fat; the position of skellysolve B inhibits HepG2 from growing; the position of acetic ester inhibits HL-60 from growing effectively.

The invention provides kansuininol compounds as shown in a formula 1 or a formula 2 in the specification. The safety of the compound 1 and the compound 2 is good, the cytotoxicity of the compound 1 and the compound 2 to different cells is small (wherein the value of IC50 is smaller than 20 [mu]M), and the compound 1 and the compound 2 have good safety; and when the compounds 1 and 2 with differentdosages are given, the inhibition of adriamycin on MCF-7 / Adr can be effectively enhanced, and a reverse multiple can reach 6.5 to 21.5. The two novel kansuininol compounds can obviously reverse the drug resistance of drug-resistant tumor cells MCF-7 / Adr, enhance the sensitivity of the tumor cells to chemotherapeutic drugs, and have good application prospects in anti-tumor multidrug resistance.

The invention belongs to the technical field of medical materials, and particularly relates to magnetic hydrogel as well as a preparation method and application thereof. The magnetic hydrogel comprises a gel network structure which is obtained by crosslinking and curing double-bond silane coupling agent modified cobalt ferrite and a double-bond monomer modified biological macromolecular material. Cobalt ferrite is modified by using a double-bond-containing silane coupling agent, so that the biocompatibility of cobalt ferrite can be effectively improved, the agglomeration of cobalt ferrite is reduced, the stability of cobalt ferrite in a system and the bonding strength between cobalt ferrite and a biological macromolecular material are enhanced, and the mechanical strength of the biological macromolecular material can be improved; meanwhile, good magnetic thermal response and heating efficiency are kept, tumor tissue is killed, and cobalt ions obtained through biodegradation have cytotoxicity and can kill tumor part cells.