19 results about "Prolactin" patented technology

The present invention provides an antibody pair that binds human mature prolactin, comprising: a monoclonal antibody 1 or an antigen-binding fragment thereof that binds to an N-terminal region of human mature prolactin, and a monoclonal antibody 2 or an antigen-binding fragment thereof that binds to a C-terminal region of human mature prolactin. The present invention also provides use of the antibody pair of the present invention in manufacturing a kit for measuring prolactin in a biological sample. The present invention also provides a monoclonal antibody or an antigen-binding fragment thereof that binds to an N-terminal region of human mature prolactin and a monoclonal antibody or an antigen-binding fragment thereof that binds to a C-terminal region of human mature prolactin, respectively. The present invention also provides a method for measuring prolactin in a biological sample using the antibody pair of the present invention. The present invention also provides a method for producing a monoclonal antibody in the antibody pair of the present invention.

This utility model relates to a wearable breast pump that mimics sucking, and relates to the field of breast pump technology. It includes a milk bowl, a sucking component, a milk bowl cover connected to the milk bowl, and a breast pump main unit. The milk bowl cover has a milk suction channel, and the sucking component is detachably connected to the milk suction channel. The breast pump main unit contains an air source device. The milk bowl has a positive pressure air path that connects the sucking component and the air source device. The air source device is connected to the sucking component through the positive pressure air path and can inflate or deflate the sucking component. When the air source device inflates the sucking component, the sucking component deforms and expands within the milk suction channel, squeezing the nipple, simulating the jaw pressing action of an infant during swallowing. Through repeated inflation and deflation, the sucking component continuously deforms, expands, and contracts within the milk suction channel. By simulating the sucking action of an infant, it can stimulate the mother's pituitary gland to secrete prolactin, thereby increasing milk production and meeting the infant's nutritional needs.

The invention belongs to the technical field of traditional Chinese medicine preparations, and discloses a haw traditional Chinese medicine ointment formula capable of reducing prolactin, promoting digestive absorption and assisting in resisting tumors and a preparation method of the haw traditional Chinese medicine ointment formula. The cream formula comprises 12-15% of Chinese dates, 10-15% of fried malt, 8-10% of lalang grass rhizome, 8-10% of chrysanthemum, 7-10% of coix seeds, 7-10% of dandelion, 5-7% of dried tangerine or orange peel, 5-7% of radix polygonati officinalis, 4-6% of hawthorn, 4-5% of Chinese yam, 4-5% of poria cocos, 3-5% of endothelium corneum gigeriae galli, 3-4% of liquorice, 2-3% of platycodon grandiflorum and 2-6% of honey. Wherein the Chinese yam and the poria cocos need to be fermented by lactobacillus acidophilus, and the dried orange peel needs to be subjected to compound enzymolysis by cellulase and pectinase. The traditional Chinese medicine composition has the effects of reducing prolactin, promoting digestion and assisting in resisting tumors. In addition, the traditional Chinese medicine composition has a protection effect on hematopoietic function damage caused by ionizing radiation. The food is suitable for dietary auxiliary conditioning of people in the tumor rehabilitation period, people with hyperprolactinemia and people with weakness of the spleen and the stomach and dyspepsia.

The present invention includes a method of treating fibrosis in a human patient in need thereof comprising administering to the human patient in need of treatment a therapeutically effective amount of Prolactin-Inducible Protein (PIP) which is capable of inhibiting or reversing the formation of pathogenic fibrils, e g., fibrosis that affects at least one organ selected from the group consisting of skin, intestine, heart, liver, lung, and kidney.

This invention discloses a detection kit for determining prolactin (PRL), comprising immunomagnetic beads, an oxidant, and a surfactant. The oxidant is a peroxidant, and the surfactant is an anionic surfactant. This detection kit for PRL utilizes an oxidant to cleave interfering substances and a surfactant to encapsulate the cleaved substances. This improves the kit's detection sensitivity, enhances its anti-interference ability when testing clinical samples, significantly improves specificity, and greatly increases clinical concordance rate. It solves the problem of falsely elevated results caused by interference from interfering substances during PRL determination.

The utility model relates to the technical field of reagent detection, and discloses a reagent tube structure for prolactin detection sample treatment, which comprises a reagent tube body, a cover body is arranged at the upper end of the reagent tube body, and the reagent tube body comprises a tubular part and a hollow conical part integrally connected with the lower end of the tubular part; a plurality of lugs distributed in the circumferential direction are arranged on the outer side of the lower end of the conical part, the lower end faces of the lugs are coplanar to form a positioning plane, and the shaft end of the reagent tube body is perpendicular to the positioning plane; the upper side face of the cover body is configured to be a supporting plane, an annular boss is arranged at the center of the lower side face of the cover body, and a plurality of clamping grooves clamped with the protruding pieces in a one-to-one correspondence mode are formed in the annular boss. The utility model has the beneficial effects that the use is more convenient, and the sample treatment efficiency is improved.

The present application relates to a kind of ammonium chloride calcium stick, belong to the technical field of feed.The ammonium chloride calcium stick includes the following components: calcium chloride, calcium sulfate, ammonium chloride, vitamin B6, modified cysteine, syringaldehyde, vitamin D3, sodium tripolyphosphate and solvent.Sodium tripolyphosphate maintains the stability of vitamin D3 and vitamin B6, wherein vitamin B6 inhibits prolactin synthesis, vitamin D3 promotes calcium absorption, prevents low blood calcium and excessive mammary secretion.The present application modifies cysteine by eugenol, increases its stability and dispersibility, and forms electrostatic interaction with sodium tripolyphosphate, forms a stable crosslinking network structure, increases the binding force between the coating layer and the raw material, prevents the loss of effective dry milk ingredients, and achieves better dry milk effect.

The invention discloses a prolactin antibody and application thereof, and relates to the field of antibodies. The prolactin antibody disclosed by the invention comprises a heavy chain complementarity determining region and a light chain complementarity determining region, provides an important raw material source for detection of prolactin, and has good affinity or activity.

The application provides a kind of based on organoid mediated drug delivery system and its application, utilize by prolactin and oxytocin stimulate mammary gland organoid has lactation function and contains a large number of lipid droplets lactation mammary gland organoid, drug-loaded organoids obtained by co-incubation of lactation mammary gland organoid and antitumor prodrug can realize efficient loading of antitumor prodrug, lactation mammary gland organoid lactation or lipid droplet lipolysis will load drug controllable release.The drug-loaded organoids provided by the application can effectively inhibit postoperative recurrence of tumor, and can promote regeneration and repair of postoperative breast tissue;The drug delivery and tissue regeneration mediated thereby can be extended to various organoids, thereby achieving treatment of various tumors and postoperative tissue repair, and having wide application prospects in tumor treatment and tissue regeneration.

This invention discloses a gene that regulates the content of gliadin in rice grains. qPro7 This invention relates to the fields of genetic engineering and plant molecular breeding, and its applications. The amino acid sequence of the gene is shown in SEQ ID NO.1. Through genome-wide association analysis, this invention identified and cloned the gene on rice chromosome 7. qPro7 By constructing genes qPro7 Knockout vectors and self-promoted overexpression vectors were used to transform rice, and the results showed that knockout vectors... qPro7 The gene can significantly reduce the content of prolactin in rice grains, while overexpression qPro7 The gene significantly increases the content of prolamins in rice grains; this invention provides new genetic resources for the genetic improvement of rice quality, especially the regulation of protein content, and has important application value.

The present invention relates to compositions for detecting a lung cancer disease, comprising peptide affinity ligands for detecting different combinations of proteins of different groups of biomarkers which include CYFRA 21-1, Prolactin, Squamous Cell Carcinoma Antigen (SCC), Her2 / Neu, Cancer antigen 125 (CA125), carcinoembryonic antigen (CEA), C- reactive protein (CRP), Neuron Specific Enolase (NSE), C4 complement (C4), Interleukin 6 (IL-6), and Metallopeptidase Inhibitor 1 (TIMP-1). The invention further relates to methods of detecting or subtyping lung diseases, as well as to computer-implemented methods based on machine learning methods using expression data of the biomarkers.

The present invention relates to a composition for diagnosing, detecting, or monitoring a liver cancer disease, comprising nucleic acid affinity ligands and / or peptide affinity ligands for a group of biomarkers comprising at least Prolactin, Alpha Fetoprotein (AFP) and Interleukin-6 (IL-6) and, optionally, further comprising one or more of Squamous Cell Carcinoma Antigen (SCC), Metallopeptidase Inhibitor 1 (TIMP-1) and CYFRA 21-1. The present invention further envisages corresponding methods for diagnosing, detecting or monitoring a cancer disease in a subject, as well as a computer-implemented method for the analysis of a sample of a subject, a computer-implemented method for providing a trained machine learning model, the use of affinity ligands for detecting, diagnosing, or monitoring a liver cancer disease, in particular early forms of liver cancer.

The invention provides an artificial propagation method for sculpin versicolor, and belongs to the technical field of fish culture. The artificial spawning induction method comprises the following steps that spawning induction hormone is injected into female parents for breeding parent fishes for three times for artificial spawning induction, the ratio of the female parent fishes to the male parent fishes is adjusted to be (2-3): 1, and fertilized eggs are obtained. The fertilized eggs are placed in a hatching basket to be hatched, a sunshade net is built above the fertilized eggs to shield light, the water temperature during hatching is 10-15 DEG C, dissolved oxygen is 5-10 mg / L, and larva fishes are obtained. According to the method, spawning induction hormone is injected in a three-needle injection mode, the injection dosage and the interval time of each needle are strictly controlled, eggs and seminal fluid which are well developed can be obtained, meanwhile, the hatching conditions of the fertilized eggs are controlled, the spawning induction rate of sculpin versicolor reaches 84.03%, the fertilization rate reaches 93.5% or above, and the hatching rate reaches 87% or above; the small-scale artificial propagation of the sculpin is realized, and the method has important significance for protecting the endangered sculpin which is an endangered resource.

This invention provides a mesenchymal stem cell culture medium, its preparation method, and its application. The mesenchymal stem cell culture medium of this invention includes a basal culture medium and functional additives. The functional additives include: recombinant human albumin, recombinant transferrin, recombinant bone morphogenetic protein; recombinant insulin or insulin-like growth factor, recombinant fibroblast growth factor, recombinant epidermal growth factor, recombinant platelet-derived growth factor; thyroid hormones, ethanolamine, carnitine, glucocorticoid regulators, retinoic acid compounds; heparin sodium, signal-regulating lipids, cholesterol, DL-α-tocopherol acetate, linolenic acid, oleic acid, stearic acid; prolactin F68, and Tween 80. The mesenchymal stem cell culture medium of this invention does not contain immunogenic proteins or blood products derived from animals or humans. All components can be supplied with GMP-compliant raw materials and the process can be controlled, ensuring the controllability, safety, and reproducibility of the culture process.

The invention discloses a prolactin antibody and application thereof, and relates to the field of antibodies. The prolactin antibody disclosed by the invention comprises a heavy chain complementarity determining region and a light chain complementarity determining region, provides an important raw material source for detection of prolactin, and has good affinity or activity.

An antibody or antigen-binding fragment thereof that binds to human prolactin (hPRL) while also neutralizing its agonistic action on human PRL receptor (hPRLR). Advantageously, this allows other hPRLR agonists to continue signal transduction. In turn, the antibody or antigen-binding fragment thereof is suitable for therapeutic use, for example, as a composition comprising the antibody or antigen-binding fragment thereof. For example, the antibodies or fragments thereof may be used to treat diseases or conditions modulated by PRL, e.g., to treat pain.

The present invention provides in vitro methods of for the production of milk-like products in a bioreactor. The method comprising providing a bioreactor comprising a culture vessel and a membrane insert that divides the culture vessel into an apical compartment and a basal compartment. A confluent layer of mammary-derived cells, optionally mammosphere-derived cells, is adhered to the apical facing surface of the membrane. Lactation media is provided to the basal compartment, optionally the lactation media comprises TGFb inhibitor, an inhibitor of intracellular actomyosin contractility such as a ROCK inhibitor or a myosin II inhibitor, and / or prolactin. Milk-like product is isolated from the apical compartment. Optionally a priming treatment is added to growth media of the hyperconfluent cell monolayer before switching to lactation.

The invention discloses an anti-prolactin antibody and application thereof, and relates to the field of antibodies. The anti-prolactin antibody disclosed by the invention comprises a heavy chain complementarity determining region and a light chain complementarity determining region, provides an important raw material source for detection of prolactin, and has good affinity or activity.

The invention provides an application of prolactin as an additive for culturing pancreas islet organs. The invention also provides a pancreas islet organ culture medium, which comprises a basic culture medium and prolactin with the concentration of 25-100 ng / mL. The invention also provides a preparation method of the pancreas islet organoid, which is characterized in that pancreas islet precursor cells are differentiated into the pancreas islet organoid in the pancreas islet organoid culture medium. The method is simple to operate, and the pancreas islet organ can be formed only by adding prolactin into a conventional culture medium. The islet organ prepared by the invention has low immunogenicity and an insulin secretion function under glucose stimulation, can normally perform a blood sugar regulation function, and can be used for treating diabetes mellitus.