102 results about "Enolase" patented technology

The invention relates to bacterial choline binding proteins (CBPs) which bind choline. Such proteins are particularly desirable for vaccines against appropriate strains of Gram positive bacteria, particularly streptococcus, and more particularly pneumococcus. Also provided are DNA sequences encoding the bacterial choline binding proteins or fragment thereof, antibodies to the bacterial choline binding proteins, pharmaceutical compositions comprising the bacterial choline binding proteins, antibodies to the bacterial choline binding proteins suitable for use in passive immunization, and small molecule inhibitors of choline binding protein mediated adhesion. Methods for diagnosing the presence of the bacterial choline binding protein, or of the bacteria, are also provided. In a specific embodiment, a streptococcal choline binding protein is an enolase, which demonstrates strong affinity for fibronectin.

The invention relates to a quantitative detection kit for NSE and a preparation method and application of the quantitative detection kit. The kit comprises a calibrator, a magnetic separation reagent, an enzyme reactant, a stable reinforcing agent and a chemiluminiscent substrate, wherein the calibrator is obtained by treating NSE antigen through a reducing agent solution and diluting the NSE antigen to a buffer solution containing a nonionic surfactant; the magnetic separation reagent is obtained by immunofixation of a biotinylation antibody and streptavidin magnetic particles; the enzyme reactant comprises a NSE tracing antibody marked by alkaline phosphatase; and the stable reinforcing agent comprises a multicomponent immune compound interfered by an anti-heterophilic antibody. The invention further relates to a preparation method of the kit and a method of applying the kit to quantitatively detect a tumor marker NSE. The kit is reliable in performance, high in flexibility, and wide in linear range, and matched up with an automatic instrument for use. At present, the kit has already obtained a third registration certificate of a diagnostic reagent in SFDA (State Food and Drug Administration).

Embodiments of the invention provides methods, devices and kits for determining the likelihood of an individual having an active fibrotic condition and/or early diagnosis, and subsequently prognosis evaluation of an individual having an active fibrotic condition such as systemic sclerosis (SSc) and/or nephrogenic systemic fibrosis (NSF) by measuring the levels of several biomarkers: α-enolase (ENO1), reticulocalbin 3 (RCN-3), alpha smooth muscle actin (α-SMA), reticulocalbin 1 (RCN-1) and pigment epithelium-derived factor (PEDF) in the individual.

Disclosed are methods for detecting a neoplasm and/or chemotherapeutic drug resistance or angiogenic potential in neoplastic cells by detecting an increase in the expression of α-enolase in such cells, or in the case of metastatic potential on the surface of such cells, as compared to the level of expression of α-enolase protein in a normal or non-MDR neoplastic cell or on the surface of a non-metastatic neoplastic cell. In addition, methods and a composition are disclosed for increasing the sensitivity of a neoplasm to a chemotherapeutic drug treatment regime, for inhibiting angiogenesis and metastatic potential in chemotherapeutic drug resistant or neoplastic cells, and for inducing apoptosis in chemotherapeutic drug resistant or neoplastic cells.

The invention discloses a marker joint detection model for diagnosis of the lung cancer. According to the invention, a carcinoembryonic antigen, a cancer antigen 125, an annexin A1 antibody and an alpha-enolase antibody are used as markers and are applied to preparation of a product for the diagnosis or assisted diagnosis of the lung cancer. According to the invention, the good optimization modelbeing optimal diagnosis combination of four kinds of biomarkers (the CEA, CA125, annexin A1 antibody and and alpha-enolase antibody) is established, wherein the e good optimization model has the better diagnosis capability superior to the single traditional marker diagnosis. The marker joint detection model as a novel method for lung cancer diagnosis has advantages of high sensitivity and specificity and has the important significance in accurate diagnosis of the lung cancer.

A method for determining whether a subject has had a stroke and, if so, the type of stroke which includes analyzing the subject's body fluid for at least four selected markers of stroke, namely, myelin basic protein, S100 protein, neuronal specific enolase and a brain endothelial membrane protein such as thrombomodulin or a similar molecule. The data obtained from the analyses provide information as to the type of stroke, the onset of occurrence and the extent of brain damage and allow a physician to determine quickly the type of treatment required by the subject.

The invention discloses a double-antibody sandwich enzymelinked immunosorbent detection kit and a preparation method of the double-antibody sandwich enzymelinked immunosorbent detection kit. The detection kit comprises a perforated plate coated with neuron-specific enolase (NSE) monoclonal antibodies and CK 19 (creatine kinase 19) monoclonal antibodies, NSE labeled by horse radish peroxidase (HRP), CK 19 labeled by alkaline phosphatase, chromogenic substrate tetramethylbenzidine (TMB), p-nitrophenyl disodium phosphate (PNPP), disodium hydrogen phosphate and substrate stop solution. The double-antibody sandwich enzymelinked immunosorbent detection kit has the advantages of being simple and convenient, sensitive, stable, good in repeatability and the like, and has practical value.

A method for determining whether a subject has had a stroke and, if so, the type of stroke which includes analyzing the subject's body fluid for at least four selected markers of stroke, namely myelin basic protein, S100 protein, neuronal specific enolase and a brain endothelial membrane protein such as thrombomodulin or a similar molecule. The data obtained from the analyses provide information as to the type of stroke, the onset of occurrence and the extent of brain damage and allow a physician to determine quickly the type of treatment required by the subject.

The invention belongs to the field of blood testing, and relates to a homogeneous phase multi-index fluorescence/chemiluminescence measuring method and application thereof. The method comprises the steps of: fixing a plurality of specificity recognition monoclonal antibodies on a polystyrene microsphere, and reacting with a plurality of labeled antibody cores modified by haptens after combining with carcinoembryonic antigens, cell keratin fragments 19, neuron specificity enolase and other multiple markers; reacting with horse radish peroxidase modified by corresponding hapten antibodies, alkaline phosphatase and quantum dots, and measuring a plurality of markers on the same instrument by combining with detections of chemiluminescence, fluorescence and the like. The method has the advantages of small blood serum consumption, rapid detection time, low diagnosis cost and simple operation, is suitable for detecting a plurality of popularized indexes simultaneously, and can provide auxiliary judgment basis for the early diagnosis of critical illnesses of tumor and the like.

The invention provides a chemiluminescence immunoassay kit for detecting neuronspecific enolase, and belongs to the technical field of in vitro diagnosis. The kit A comprises a calibrator, a magneticparticle reaction liquid Ra1, a trace binder reaction liquid Ra2 and a capture antibody reaction liquid Ra3. The kit B comprises a calibrator, a magnetic particle reaction liquid Rb1, a trace binder reaction liquid Rb2 and a project dilution reaction liquid Rb3. The kit 3 comprises a calibrator, a magnetic particle reaction liquid Rc1 and a trace binder reaction liquid Rc2. The kit has wide detection linear range, high sensitivity and good specificity, is an automatic chemiluminescence instrument mated reagent, and is applicable to quantitative detection of NSE in serum and plasma. According to the kit, consistent detection on serum and EDTA plasma samples can be realized by adding a proper amount of Mg<2+> ions, and the serum and plasma measured value related coefficient r is more than 0.975.

The invention discloses an extraction method and an application of a component group of a traditional Chinese medicine extract for treating hemorrhagic stroke. The extraction method comprises the following steps: after crushing rheum officinale, cortex moutan, red peony roots, dried radix rehmanniae and pseudo-ginseng in a certain weight ratio, adding water to decoct and extract; after extracting an extraction liquid by using dichloromethane, extracting a water layer by using n-butyl alcohol; and recovering and drying the n-butyl alcohol extraction liquid to obtain the component group of the traditional Chinese medicine extract. Analyzed by HPLC-MS, the traditional Chinese medicine extract is prepared from 33 components: paeoniflorins, tannins, paeonosides, anthraquinones, stilbenes, saponins and iridoid glycosides and can be used for remarkably reducing levels of neuronspecific enolase of essential hypertension male rats, S-100beta proteins, IL-1, TNF-alpha and plasma fibrinogen and can be used for preparing the medicine for treating hemorrhagic stroke.

The invention relates to a method for determining the presence of renal damage in an individual and also to a method for detecting one or several proteins selected from the list comprising Reg3B, fetuin B, Ras-related GTP-binding protein A serine protease inhibitor A3L, subunit 1 of COP9, gamma subunit of ATP synthase, gelsolin, ribonuclease UK114, aminoacylase 1A, alpha-enolase, keratin 5, parvalbumin alpha, ribonuclease 4 or serine protease inhibitor A3K. The renal damage may be acute renal failure. Said renal pathologies may be caused by the administration of a nephrotoxic agent, wherein the nephrotoxic agent may be an aminoglycoside antibiotic such as gentamicin, or cisplatin. The invention also provides means to differentiate the renal damage or renal failure induced by gentamicin from that induced by cisplatin, through the biochemical analysis of the urinary level of Reg3B and/or gelsolin, or fragments thereof.

This invention involves a cis-dihydrojasmone acid methyl ester synthetic method. The existing multi-step synthesis method is low yield and product content. This invention takes ordinary dihydrojasmone acid methyl ester as raw material, by enolase reaction and halogenation reaction, to dehalogenate to prepare double bonds jasmonate methyl ester; finally, under the solvent-free state through hydrogenation to obtain cis-dihydrojasmone acid methyl ester.

The invention relates to the medical field of immunoassay, more specially, the invention provides a chemiluminescent immunoassay detection kit for nerve specific enolase and a preparation method thereof. The kit of the invention comprises 1) nerve specific enolase calibrators, 2) solid-phase vectors which are coated by nerve specific enolase monoclonal antibodies, 3) nerve specific enolase monoclonal antibody antigens which are marked by enzyme and 4) chemiluminescent substrates which are acted by the enzyme. The invention also provides a preparation method of the kit, which comprises the steps: preparing the calibrators, coating the vectors, marking the antibodies, packaging, and the like. The kit of the invention has the advantages of convenience, rapidness, sensitivity, stability, and the like. The invention can provide good technical support for the early detection, the detection of disease progress and the judgment of healing effect of some diseases such as small cell lung carcinoma (SCLC), neuroblastoma, brain damage, and the like.

The invention discloses mycoplasma bovis enolase and new applications of a coding gene thereof. Specifically, the invention discloses the mycoplasma bovis enolase and applications of the coding gene of the mycoplasma bovis enolase in preparing reagents for detecting plasminogen and detecting or diagnosing infections caused by mycoplasma bovis. According to the mycoplasma bovis enolase disclosed by the invention, an enolase protein is obtained through prokaryotic expression, and soluble expression is successfully performed on the protein through regulating induction conditions, and further, through recombinant enolase protein and plasminogen ligand experiments and enolase protein and mycoplasma bovis antibody Western blot experiments, the protein is finally proved to have the binding activity and the immunogenicity of the plasminogen, therefore, the protein can be used for detecting the plasminogen and detecting or diagnosing the infections or diseases caused by the mycoplasma bovis. Moreover, a new path is also opened up for researching the adhesion mechanism and the pathogenicity of the mycoplasma bovis and developing vaccines in future.

The invention belongs to a method for identification and purification of intracellular enolase. The method comprises the method for identifying and purifying the intracellular enolase. The method comprises the following steps that: 0.5 g (wet weight) of collected bacteria or cells are suspended by using 0.5 ml of a PBS buffer; treatments of electrophoresis and gel stripping are performed; the resulting gel sheet is subjected to activity staining; a small amount purification treatment is performed by a dialysis method to obtain the enolase; and the like. With the present invention, the intracellular enolase can be intuitionally identified and purified, and the method has characteristics of simple operation, short experimental period, low price, economy and practicality.

The present invention relates to methods and pharmaceutical compositions for the treatment of autoimmune diseases, especially rheumatoid arthritis. More particularly, the present invention relates to an a-enolase polypeptide for use in the prophylactic treatment of an autoimmune disease in a subject in need thereof.

The invention discloses an anti-alpha-enolase antibody chromatography test strip, and a preparation method and application thereof. The test strip includes a sample pad (1), a combination pad (2), an analysis membrane (3), a water absorption pad (6), a base plate (7), a test zone T line (4) and a quality control C line (5). The upper part of the base plate (7) is adhered to the analysis membrane (3), and the the upper two ends of the analysis membrane (3) are respectively adhered to the combination pad (2) and the water absorption pad (6); one upper end of the combination pad (2) is adhered to the sample pad (1); the test zone T line (4) and the quality control C line (5) are arranged on the analysis membrane (3). The invention employs indirect immunodetection and alpha-enolase antigen protein to realize high sensitivity, high specificity and rapid detection of alpha-enolase antibody protein and provide the basis for auxiliary diagnosis of clinical autoimmune diseases such as inflammatory bowel disease.

The invention relates to a hybridoma for secreting an anti-recombinant schistosoma japonica enolase specific monoclonal antibody as well as a preparation method and application of the hybridoma, and belongs to the technical fields of gene engineering, preparation of monoclonal antibodies and immunologic diagnosis. The hybridoma JYG-1 capable of secreting the anti-recombinant schistosoma japonica enolase (Sj Enolase protein) specific monoclonal antibody is preserved in the Common Microbiology Center of CCCCM (China Committee for Culture Collection of Microorganisms) and has the preservation number of CGMCC NO. 9910. The hybridoma JYG-1 can secrete the anti-recombinant schistosoma japonica enolase (Sj Enolase) specific monoclonal antibody JYGENmb-1. The monoclonal antibody JYGENmb-1 can be combined with Sj Enolase protein specificity of schistosoma japonica, a sandwich ELISA method for detecting Sj Enolase protein is provided, and the monoclonal antibody can be applied to establishing various immunological methods for detecting Sj Enolase protein in schistosoma japonica infected human and animal blood and excreting secretion.