15 results about "Glyceraldehyde" patented technology

The present invention relates to a use of an enzyme belonging to the aldolase group and comprising a polypeptide having at least 30% identity with an amino acid sequence selected from SEQ ID NO: 1 to SEQ ID NO: 4, for stereoselectively preparing D-tagatose-1,6-bisphosphate (TBP) or D-tagatose-1-phosphate. The present invention also relates to a method for preparing D-tagatose-1,6-bisphosphate (TBP) or D-tagatose-1-phosphate, comprising a step of reacting, in a suitable reaction medium, dihydroxyacetone phosphate (DHAP) or at least an isomer thereof or analog thereof, and optionally D-glyceraldehyde, with an enzyme belonging to the aldolase group, said enzyme comprising a polypeptide having at least 30% identity with an amino acid sequence selected from SEQ ID NO: 1 to SEQ ID NO: 4. Finally, the present invention relates to a process for preparing D-tagatose.

This invention relates to the field of chemical synthesis technology, specifically disclosing a method for preparing vinyl ethylene carbonate. The method uses glycidyl as a starting material, first converting it to glycidaldehyde via a mild oxidation reaction with high selectivity. Then, using a cross-linked imidazole polymeric ionic liquid as a catalyst, glycidaldehyde undergoes an atom-economical cycloaddition reaction with carbon dioxide to construct a cyclic carbonate intermediate. Finally, under catalytic conditions, a condensation reaction introduces a vinyl functional group to obtain the vinyl ethylene carbonate product. This invention uses widely available, low-toxicity, and inexpensive glycidyl instead of highly toxic raw materials, and utilizes greenhouse gas carbon dioxide as a carbonyl source for resource utilization. The process route is highly atom-economical, the reaction conditions are mild, and the catalyst is easy to separate, recover, and recycle, effectively solving the prominent problems of high raw material toxicity, expensive catalysts, and difficult byproduct treatment in traditional preparation techniques.

The invention relates to the technical field of microorganisms, and particularly discloses a construction method and application of a recombinant microorganism. According to the construction method of the recombinant microorganism, compared with an original strain, the expression quantity of threonine operon leader peptide of the recombinant microorganism is reduced, and the expression quantities of glyceraldehyde-3-phosphate dehydrogenase mutant, aspartate kinase / homoserine dehydrogenase I mutant, homoserine kinase and threonine synthase are increased; the glyceraldehyde-3-phosphate dehydrogenase mutant is characterized in that glycine at the 188th site in a glyceraldehyde-3-phosphate dehydrogenase protein sequence is mutated into serine; the aspartate kinase / homoserine dehydrogenase I mutant is characterized in that glycine at the 433rd site in an aspartate kinase / homoserine dehydrogenase I protein sequence is mutated into arginine; the starting strain is escherichia coli. According to the construction method disclosed by the invention, a novel recombinant microorganism with improved threonine yield can be obtained, and a novel method is provided for fermentation production of threonine.

The application discloses an immunogen for preparing testis-specific glyceraldehyde-3-phosphate dehydrogenase lysine malonylation site-specific antibody, and belongs to the technical field of biotechnology.The immunogen is a malonylation modified polypeptide, and the amino acid sequence is IIPASTGAAKAVT, corresponding to the 278th-290th amino acid of a human GAPDHS protein, wherein a lysine at the 287th position (the 10th position in the sequence) is subjected to malonylation modification.A specific antibody designed according to the immunogen has the characteristics of high titer and high specificity. By screening a human sperm sample by using the antibody, it is found that the antibody can not only specifically recognize the GAPDHS with the lysine at the 287th position subjected to malonylation modification in vitro and in human sperm, but also is closely related to sperm forward movement and asthenozoospermia. The application has great significance in the field of preparation of reagents for precision diagnosis and treatment of idiopathic male infertility.

The application discloses a method for fermenting 3-hydroxypropionic acid and acrylic acid. The application provides a recombinant Corynebacterium glutamicum, wherein the ald gene of the aldehyde dehydrogenase of the microorganism is up-regulated, the expression of the glycerolaldehyde-3-phosphate dehydrogenase gapA is down-regulated, the heterologous diol dehydratase gene pduCDEGH is expressed, the heterologous 3-phospho dehydrogenase gdp and glycerol 3-phosphatase gpp are expressed. The recombinant microorganism is fermented in a shake flask or a fermenter with glucose or other organic carbon sources as the substrate to obtain 3-hydroxypropionic acid. The 3-hydroxypropionic acid in the fermentation broth is acidified and heated to further obtain acrylic acid. The recombinant microorganism can efficiently produce 3-hydroxypropionic acid and acrylic acid by using cheap glucose, sucrose, molasses and the like as raw materials, the production process is green, safe and simple, and has a good market application prospect.

The present application relates to the field of bioengineering technology, and more particularly to a kind of tiger frog environment detection sample storage reagent and its preparation method, consisting of the following components by mass fraction: preservative 95-99 parts and microcapsule 1-5 parts;The present application is composed of the composite system of environmental protection type preservative matrix and temperature-sensitive microcapsule encapsulating glycerol aldehyde-3-phosphate dehydrogenase inhibitor by design, so that the reagent has the double-stage temperature change response function of "in situ slow release under normal temperature transportation to stabilize sample microbial metabolic state" and "incubation triggered rapid release in laboratory", can maximize the preservation of tiger frog environmental sample collection instant microbial activity and community structure information, thereby significantly improving the authenticity and reliability of subsequent molecular detection results, providing an efficient and convenient tool for tiger frog habitat health assessment, biodiversity monitoring and ecological environment research, and has broad application prospect.

The application belongs to the technical field of biological medicine, and discloses application of oridonin in preparation of a medicine for treating rheumatoid arthritis synovitis. For the first time, it is confirmed that oridonin takes glyceraldehyde-3-phosphate dehydrogenase as a direct action target, covalently combines through an α, β-unsaturated ketone, inhibits enzyme activity, blocks abnormal glycolysis of rheumatoid arthritis synovial cells, activates a KEAP1-NRF2 / HO-1 antioxidant anti-inflammatory pathway, inhibits an NF-κB inflammatory signal, and intervenes in a pathological process of rheumatoid arthritis synovitis from multiple dimensions, thereby providing a scientific basis for clinical application and deep development of oridonin against RA synovitis. A medicine composition containing oridonin and application of the medicine composition in preparation of an anti-rheumatoid arthritis synovitis medicine are disclosed. The application has advantages of target point precision, remarkable curative effect, high safety, mechanism innovation and the like, and provides a brand-new scheme for clinical treatment of rheumatoid arthritis synovitis.

The present disclosure concerns recombinant yeast host cells having a first genetic modification for downregulating a first metabolic pathway that converts NADP+ to NADPH, as well as a second genetic modification for upregulating a second metabolic pathway that converts NADP+ to NADPH. The second genetic modification allows the expression of a glyceraldehyde-3-phosphate dehydrogenase lacking phosphorylating activity, which can, in some embodiments, be from enzyme commission 1.2.1.9 or 1.2.1.90. The second pathway is distinct from the first metabolic pathway. The present disclosure also concerns a process for making and improving the yield of a fermented product, such as ethanol, using the recombinant yeast host cell.

The invention discloses an antibacterial adhesive and a preparation method thereof, and relates to the field of adhesives. When the antibacterial adhesive is prepared, a flame retardant precursor and glycidyl aldehyde react to prepare a flame retardant monomer; reacting the flame retardant monomer with 5-aminoresorcinol to prepare a flame-retardant cross-linking agent; the preparation method comprises the following steps: reacting 4-(4-hydroxyphenyl)-1-(2H)-phthalazinone with 2-bromoethanol to prepare a thermal stable monomer; the preparation method comprises the following steps: reacting 5, 5-dimethyl hydantoin with 3, 3-dimethyl-1-chlorobutane to obtain an antibacterial precursor; reacting an antibacterial precursor with the pre-modified montmorillonite, and adding sodium hypochlorite for chlorination to prepare modified montmorillonite; polymerizing a thermal stable monomer, polyethylene glycol and isophorone diisocyanate, and adding the modified montmorillonite and the flame-retardant cross-linking agent, so as to obtain the antibacterial adhesive. The antibacterial adhesive prepared by the invention has excellent antibacterial, flame-retardant and high-temperature-resistant properties.

This invention provides a glyceraldehyde-3-phosphate dehydrogenase mutant and its application, the mutant being based on Streptococcus mutans Source gapN The original sequence was used to obtain information about rational mutation design. gapN The effective site mutation of the amino acid coding sequence was obtained, and through gene editing, the coding genes of the wild-type sequence, the mutant coding gene, and the mutant combination were integrated into the genome of *Corynebacterium glutamicum* to obtain a new strain. The lysine production of the new strain was significantly increased, further demonstrating that... gapN Mutations at three sites in the gene have a significant effect on lysine production, providing important gene targets and engineering strategies for constructing industrial strains that produce L-lysine efficiently.

The present invention relates to a use of an enzyme belonging to the aldolase group and comprising a polypeptide having at least 30% identity with an amino acid sequence selected from SEQ ID NO: 1 to SEQ ID NO: 4, for stereoselectively preparing D-tagatose-1,6-bisphosphate (TBP) or D-tagatose-1-phosphate. The present invention also relates to a method for preparing D-tagatose-1,6-bisphosphate (TBP) or D-tagatose-1-phosphate, comprising a step of reacting, in a suitable reaction medium, dihydroxyacetone phosphate (DHAP) or at least an isomer thereof or analog thereof, and optionally D-glyceraldehyde, with an enzyme belonging to the aldolase group, said enzyme comprising a polypeptide having at least 30% identity with an amino acid sequence selected from SEQ ID NO: 1 to SEQ ID NO: 4. Finally, the present invention relates to a process for preparing D-tagatose.

An integrated structure of cocatalyst / heterogeneous structure / tungsten trioxide photoanode, its preparation method, and its application are disclosed, belonging to the fields of biomass conversion and photoelectrocatalysis. First, a zinc oxide / tungsten trioxide heterostructure is synthesized via hydrothermal, spin-coating, and calcination methods. Then, a noble metal-modified nickel phosphide cocatalyst is synthesized via phosphating and loaded onto the surface of the heterostructure to successfully construct an integrated system of cocatalyst / heterogeneous structure / photoanode. The zinc oxide and tungsten trioxide heterostructure can suppress the recombination of photogenerated carriers, while the noble metal-modified cocatalyst can reduce the interfacial resistance between the heterostructure and the electrolyte, accelerate the migration of photogenerated holes, significantly reduce the surface energy barrier, improve surface reaction kinetics, and prolong the lifetime of photogenerated charges. This results in excellent photoelectrocatalytic glycerol oxidation performance, producing only two C3 products: glyceraldehyde and 1,3-dihydroxyacetone. The limited product diversity provides a feasible pathway for improving the high-value coupling of photoelectrocatalytic biomass to hydrogen production.

The present invention relates to a process for producing a foam, which comprises foaming a mixture, comprising one or more poly (amino acid) (A), one or more components (B) capable of reacting with said poly (amino acid) (A), wherein component (B) is selected from reducing sugars, 1,3-dihydroxyacetone, glycolaldehyde, glyceraldehyde or any mixture thereof and one or more blowing agents (F) and the foam obtainable by this process.

The invention relates to an environment-friendly corrosion inhibitor based on water-soluble cardanol, and relates to the field of oil field corrosion inhibitor preparation. The problems that an existing corrosion inhibitor is poor in environmental protection property and inconvenient to operate are mainly solved. The anacardol composition comprises the following raw material components in percentage by mass: 5-20% of anacardol extract; 1%-10% of glycidyl aldehyde; 2%-15% of acetic acid; 0.05%-0.5% of a catalyst; 2%-10% of a water-soluble auxiliary agent; the preparation method comprises the following steps: (1) preparing the cardanol extract; and (2) carrying out condensation polymerization on the cardanol extract, glycidyl aldehyde, acetic acid and a catalyst in proportion under reaction conditions to obtain the cardanol-based corrosion inhibitor, (3) adding a water-soluble additive in the polycondensation reaction process; and (4) separating, washing, drying and grinding the obtained corrosion inhibitor to obtain purified corrosion inhibitor powder based on water-soluble cardanol. The environment-friendly corrosion inhibitor meets the dual requirements of oil field application on environmental protection and corrosion inhibition performance.

The invention discloses a composition for treating alopecia and a preparation method thereof.The composition is prepared from, by mass, 0.3%-0.5% of nano-particle mixture, 3%-5% of glyceraldehyde, 0.3%-0.5% of tanshinone IIA, 0.3%-10% of water-soluble vitamin E and the balance castor oil, the nano particle mixture is a mixture of anatase type TiO nano powder and monoclinic zinc type ZnO nano powder. Through the synergistic effect of multiple components, the effects of improving scalp blood circulation, supplementing hair follicle nutrition and activating hair follicle cells are synchronously achieved, and therefore alopecia is efficiently treated.