66 results about "Glyceraldehyde" patented technology

The invention discloses a light-driven synthetic flora and application thereof. The light-driven synthetic flora comprises phototrophic bacteria and heterotrophic bacteria, the phototrophic bacteria are genetically modified synechococcus slender, and the gene modification comprises construction and expression of heterologous sucrose permease, glyceraldehyde dehydrogenase and sucrose synthase in the synechococcus slender; and the heterotrophic bacteria are the P.adami. According to the light-driven synthetic flora disclosed by the invention, carbon dioxide is converted into cane sugar through photosynthesis of phototrophic bacteria, and the cane sugar is secreted out of cells to provide a carbon source for heterotrophic bacteria; carrying out cell growth and polyhydroxyalkanoate synthesis on the heterotrophic bacteria by using cane sugar; therefore, the light-driven synthetic flora provided by the invention can realize direct conversion of carbon dioxide into polyhydroxyalkanoate without additional carbon sources, has the advantage of environmental friendliness, and provides a new economical and feasible scheme and approach for production of polyhydroxyalkanoate.

The invention provides human trichomonad antigen gene protein glyceraldehyde-3-phosphate dehydrogenase, and particularly relates to application of the protein in immunological detection of human trichomonad infection, the protein can be used for immunological detection of human trichomonad infection, and a human trichomonad detection kit is prepared. The GAPDH protein is used as a detection antigen for indirect ELISA detection, and the method has the characteristics of high sensitivity and specificity.

The invention provides a bimetallic hierarchical pore catalyst as well as a preparation method and application thereof, and belongs to the technical field of catalyst materials. The preparation method comprises the following steps: firstly, preparing a hierarchical pore ZSM-5 molecular sieve by taking organic alkali, a ZSM-5 molecular sieve and ammonia water as raw materials, improving the selectivity of a target product glyceraldehyde and prolonging the service life of a catalyst by virtue of a rich pore structure of the hierarchical pore ZSM-5 molecular sieve, and then loading metal M1 and metal M2 on the surface of the hierarchical pore ZSM-5 molecular sieve, the reaction activity and selectivity of the prepared catalyst for catalyzing polyol oxidation are enhanced by utilizing the synergistic effect of bimetals, the polyol oxidation reaction can be efficiently catalyzed under mild conditions to generate glyceraldehyde, and finally the bimetal hierarchical pore catalyst with excellent glyceraldehyde selectivity and catalytic stability is obtained. The defects of low glyceraldehyde selectivity, short catalyst life and the like in the prior art are overcome.

The application discloses a duck Riemerella anatipestifer (RA) surface adhesion factor 3-glyceraldehyde-3-phosphate dehydrogenase protein (GAPDH) conservative B cell epitope peptide, a nucleic acid molecule, a recombinant carrier and application thereof. The application inserts the conservative B cell epitope TG12 into chicken white dysentery Salmonella Peg fimbria, introduces the inert carrier bacteria, and obtains the recombinant bacteria which can functionally express and present the RaGAPDH protein surface conservative B cell epitope peptide. The expression and presentation of the conservative B cell epitope can specifically recognize and combine duck and goose RA infection serum, and the naked-eye visible agglutination reaction particles are observed, and there is no cross agglutination reaction with other pathogen infection positive serum. The agglutination detection method has the advantages of specificity and convenience, and is expected to provide a new idea and method for detection and prevention and control of RA infection.

The application discloses a preparation method of an octene aldehyde liquid-phase hydrogenation catalyst, and the preparation method comprises the following steps: (1) adding a weak acid into a salt solution containing silver, magnesium and aluminum, uniformly stirring, then adding a glycerol aldehyde, continuously stirring until a colloid is formed, grinding the colloid to obtain a powder, sieving, and roasting to obtain a carrier; and (2) preparing a precursor solution containing nickel and zinc, placing the precursor solution and the carrier in an ultrasonic container to shake, pouring the shaken solution into a well-sealed ball mill tank, ball milling, and then drying and roasting to obtain the catalyst. The catalyst is prepared by adding a silver-containing compound when the carrier is prepared and the ball milling method, so that the selectivity and stability of the catalyst are improved.

The present invention relates to a use of an enzyme belonging to the aldolase group and comprising a polypeptide having at least 30% identity with an amino acid sequence selected from SEQ ID NO: 1 to SEQ ID NO: 4, for stereoselectively preparing D-tagatose-1,6-bisphosphate (TBP) or D-tagatose-1-phosphate. The present invention also relates to a method for preparing D-tagatose-1,6-bisphosphate (TBP) or D-tagatose-1-phosphate, comprising a step of reacting, in a suitable reaction medium, dihydroxyacetone phosphate (DHAP) or at least an isomer thereof or analog thereof, and optionally D-glyceraldehyde, with an enzyme belonging to the aldolase group, said enzyme comprising a polypeptide having at least 30% identity with an amino acid sequence selected from SEQ ID NO: 1 to SEQ ID NO: 4. Finally, the present invention relates to a process for preparing D-tagatose.

Disclosed herein are genetically engineered yeast cells capable of producing xylitol and comprising a glyceraldehyde-3-phosphate dehydrogenase enzyme at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% identical to SEQ ID NO:100. The genetically engineered yeast cell may additionally have a deletion or disruption of a native gene encoding an erythrose reductase and may be engineered to overexpress a native RPE enzyme, express an exogenous XPDH enzyme, express an exogenous XKS enzyme, express an exogenous XDH enzyme, overexpress a native X5PP enzyme, and / or express an exogenous X5PP enzyme.

The present invention relates to a process for producing 4-hydroxymethylfurfural (HMF) comprising the steps of: converting 4-hydroxymethylfurfural-phosphate to 4-hydroxmethylfurfural using a phosphatase; and a process for producing 4-HMF from a feedstock comprising a carbon source, comprising the steps of converting 4-hydroxymethylfurfural-phosphate to 4-hydroxmethylfurfural using a phosphatase and an enzyme cascade comprising i.a. synthases to convert fructose-6-phosphate to glyceraldehyde-3-phosphate; wherein the carbon source is preferably selected from the group consisting of starch, dextrines, cellulose, sucrose and / or combinations thereof.

The present invention discloses a glyceraldehyde 3-phosphate dehydrogenase mutant and its application in increasing the yield of O-succinyl-L-homoserine, wherein the mutant is obtained by single mutation of the arginine at position 233 of the amino acid sequence shown in SEQ ID NO.2. The present invention uses recombinant Escherichia coli producing O-succinyl-L-homoserine as the target strain, introduces exogenous glyceraldehyde 3-phosphate dehydrogenase gapC and its mutant to provide more NADPH supply for the metabolic pathway to increase the yield of OSH. The wild-type gapC provided by the present invention is expressed in recombinant Escherichia coli and can increase the yield of OSH in a 5L fermentor from the original 87g / L to 102g / L, and after the gapC mutant is expressed in recombinant Escherichia coli, the yield of OSH in a 5L fermentor increases to 135g / L, and the supply of NADPH is increased by 50% over the original strain.

This invention relates to the field of chemical synthesis technology, specifically disclosing a method for preparing vinyl ethylene carbonate. The method uses glycidyl as a starting material, first converting it to glycidaldehyde via a mild oxidation reaction with high selectivity. Then, using a cross-linked imidazole polymeric ionic liquid as a catalyst, glycidaldehyde undergoes an atom-economical cycloaddition reaction with carbon dioxide to construct a cyclic carbonate intermediate. Finally, under catalytic conditions, a condensation reaction introduces a vinyl functional group to obtain the vinyl ethylene carbonate product. This invention uses widely available, low-toxicity, and inexpensive glycidyl instead of highly toxic raw materials, and utilizes greenhouse gas carbon dioxide as a carbonyl source for resource utilization. The process route is highly atom-economical, the reaction conditions are mild, and the catalyst is easy to separate, recover, and recycle, effectively solving the prominent problems of high raw material toxicity, expensive catalysts, and difficult byproduct treatment in traditional preparation techniques.

The invention relates to the technical field of microorganisms, and particularly discloses a construction method and application of a recombinant microorganism. According to the construction method of the recombinant microorganism, compared with an original strain, the expression quantity of threonine operon leader peptide of the recombinant microorganism is reduced, and the expression quantities of glyceraldehyde-3-phosphate dehydrogenase mutant, aspartate kinase / homoserine dehydrogenase I mutant, homoserine kinase and threonine synthase are increased; the glyceraldehyde-3-phosphate dehydrogenase mutant is characterized in that glycine at the 188th site in a glyceraldehyde-3-phosphate dehydrogenase protein sequence is mutated into serine; the aspartate kinase / homoserine dehydrogenase I mutant is characterized in that glycine at the 433rd site in an aspartate kinase / homoserine dehydrogenase I protein sequence is mutated into arginine; the starting strain is escherichia coli. According to the construction method disclosed by the invention, a novel recombinant microorganism with improved threonine yield can be obtained, and a novel method is provided for fermentation production of threonine.

The invention discloses an application of a zeolite imidazole framework material in catalysis of a carbon-carbon condensation reaction of formaldehyde. The zeolite imidazole framework material is selected from ZIF-Co / IM, ZIF-Co / 2-MIM, ZIF-Co / 2-EIM, ZIF-Co / 2-PIM, ZIF-Co / 2-BIM, ZIF-Co / 4-MIM, ZIF-Co / dClIM, ZIF-Co / Pur, ZIF-Co / BzIM, ZIF-Co / 2-NIM or ZIF-Zn / 2-MIM. The zeolite imidazole skeleton nano-enzyme material can catalyze formaldehyde FA to generate carbon-carbon condensation so as to generate high value-added saccharide small molecules. Through comparison with a high performance liquid chromatogram of a standard substance, catalytic products are mainly C3 compounds glyceraldehyde (GCA) and dihydroxyacetone (DHA), and a small amount of C4 compounds comprise erythritol, threonose and ketulose. Compared with the traditional formaldehyde enzyme, the formaldehyde enzyme has the advantages of high-concentration substrate tolerance, high-temperature resistance and the like.

The invention discloses a TiO2 (at) cMOF heterojunction photo-anode material as well as a preparation method and application of the TiO2 (at) cMOF heterojunction photo-anode material. The TiO2 nanorod array photo-anode material TiO2 (at) cMOF coated with a single-metal conductive metal organic framework (cMOF) and a double-metal conductive metal organic framework (cMOF) is prepared by adopting a solvothermal method, and the selectivity for synthesizing glyceraldehyde through photoelectrocatalytic oxidation of glycerol in a neutral medium is high; the photo-anode has a unique core-shell structure, the separation efficiency of photon-generated carriers is enhanced, further the photoelectrocatalytic activity of glycerin is remarkably improved, biomass oriented upgrading of converting glycerin into glyceraldehyde is achieved, meanwhile, the photoelectrocatalytic reaction condition is mild, the problem of C-C bond breakage in the oxidation process of multi-carbon organic matter can be effectively solved, and the photo-anode has a good application prospect. A new thought is provided for high-selectivity preparation of a multi-carbon product.

The invention discloses a chromium-free and formaldehyde-free synergistic tanning method for high-flexibility ecological Tan sheep skin, which comprises the following steps: firstly, pre-tanning the Tan sheep skin by using a 6-10g / L biomass-based aldehyde tanning agent, and then carrying out zirconium tanning and synergistic tanning to obtain chromium-free and formaldehyde-free tanned Tan sheep skin. The bio-based aldehyde tanning agent is prepared through selective oxidation of glycerin, the structure of the bio-based aldehyde tanning agent contains aldehyde groups and hydroxyl groups, polyglyceraldehyde with a long-chain structure can be formed through polymerization by adjusting the pH value, and then the polyglyceraldehyde is covalently cross-linked with collagen amino groups; the introduction of the zirconium tanning agent not only can be complexed with collagen carboxyl, but also can be coordinated with hydroxyl on the structure of the biomass-based aldehyde tanning agent to form a multi-point cross-linked network, so that the flexibility and damp-heat resistance stability of the Tibet lamb skin are remarkably improved, and meanwhile, the health and environmental risks caused by chromium salt and formaldehyde are thoroughly eliminated.

The invention discloses an application of a substance for detecting a marker group. The application comprises one or more of the following applications: A1) an application in preparation of a product for predicting sensitivity of bicitinib combined with narrow-spectrum medium-wave ultraviolet rays in treatment of leucoderma, and A2) an application in preparation of a product for predicting sensitivity of bicitinib combined with narrow-spectrum medium-wave ultraviolet rays in treatment of leucoderma; a2) is applied to preparation of a product for screening sensitivity of bicitinib combined with narrow-spectrum medium-wave ultraviolet rays in treatment of leucoderma; a3) in preparation of a product for evaluating sensitivity of bicitinib combined with narrow-spectrum medium-wave ultraviolet rays in treatment of leucoderma; the marker group is composed of CCL5, FGL1, EZR and GAPDH (glyceraldehyde-phosphate dehydrogenase). The invention screens and verifies the plasma and urine biomarker group capable of predicting the sensitivity of treating leucoderma by combining baricitinib with narrow-spectrum medium-wave ultraviolet rays of a subject through ELISA (Enzyme-Linked Immunosorbent Assay), and the plasma and urine biomarker group provided by the invention has higher prediction accuracy, sensitivity and specificity; the individualized treatment of vitiligo can be improved, and good clinical application and popularization prospects are achieved.

The present invention relates to a system for producing an in-situ foam comprising one or more poly (amino acids) (A), one or more components (B) capable of reacting with said poly (amino acids) (A) and one or more amphoteric polymers (C) wherein component (B) is selected from the group consisting of reducing sugars, 1, 2, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, 3, and 2, 3-dihydroxyacetone, glycolaldehyde, glyceraldehyde, or any mixture thereof.

The application discloses an immunogen for preparing testis-specific glyceraldehyde-3-phosphate dehydrogenase lysine malonylation site-specific antibody, and belongs to the technical field of biotechnology.The immunogen is a malonylation modified polypeptide, and the amino acid sequence is IIPASTGAAKAVT, corresponding to the 278th-290th amino acid of a human GAPDHS protein, wherein a lysine at the 287th position (the 10th position in the sequence) is subjected to malonylation modification.A specific antibody designed according to the immunogen has the characteristics of high titer and high specificity. By screening a human sperm sample by using the antibody, it is found that the antibody can not only specifically recognize the GAPDHS with the lysine at the 287th position subjected to malonylation modification in vitro and in human sperm, but also is closely related to sperm forward movement and asthenozoospermia. The application has great significance in the field of preparation of reagents for precision diagnosis and treatment of idiopathic male infertility.

A set of serum metabolic biomarkers and detection kit for detecting drug-resistant tuberculosis are provided. The set of the serum metabolic biomarkers includes 17 serum metabolic biomarkers. The 17 serum metabolic biomarkers are verified to be associated with tuberculosis based on the level changes of these biomarkers. The 17 metabolites includes taurine, homocysteine, uric acid, ascorbic acid, suberylglycine, uridine, dopamine 4-sulfate, inosinic acid, glyceraldehyde phosphate, [3-methoxy-4-(phosphoryl)phenyl]carbonyl sulfonic acid, nuclomedone, n4-cyclopropyl-6-(2,3-dichlorophenyl)-1,2,3,4-tetrahydropyrimidine-2,4-diimine, 1-[2-chlorine-2-(2,4-dichlorophenyl)ethenyl]-1,2,4-triazole, tetrachloro-phthalic anhydride, malotilate, fulvic acid, and L-neopterin. The serum metabolic biomarkers and detection kit for detecting drug-resistant tuberculosis can assist doctors in accurately diagnosing the disease, which is of great significance for the diagnosis and mass screening for drug-resistant tuberculosis.

The invention relates to the technical field of biologic detection of metabolomics, in particular to an OSAHS metabolism marker, a diagnosis model and application of the OSAHS metabolism marker and the diagnosis model. The metabolic marker comprises a red blood cell metabolite and / or a plasma metabolite; erythrocyte metabolites comprise L-aspartic acid, D-ribose-5-diphosphoric acid, cadaverine, 12, 13-epoxy octadecenoic acid, L-arginine, N-acetylmethionine, 5-hydroxyeicosatetraenoic acid, 13-oxo-9Z, 9, 10-tetrahydroxy-9Z, 9, 10-tetrahydroxy-9Z, 9, 10-tetrahydroxy-9Z, 9, 10-tetrahydroxy-9Z, 9, 10-tetrahydroxy-9Z, 10-tetrahydroxy-9Z, the component A is one or more of 11E-octadecadienoic acid, inosine monophosphate, nicotinamide, alpha-lactose, sphingosine, hydroxy octadecadienoyl or glyceraldehyde-3-phosphoric acid; the plasma metabolite is prepared from one or more of fumaric acid, cadaverine, L-aspartic acid, malic acid, sphingosine-1-phosphoric acid, L-arginine, cysteine-S-sulfate, thiosulfate, gamma-glutamyl alanine, galacturonic acid or vitamin B2.

The present invention is directed to providing a monoclonal antibody having high selectivity and affinity for AGEs, particularly, AGEs derived from glyceraldehyde or AGEs derived from glycolaldehyde, and an analysis method utilizing the same. Another object of the present invention is to provide methods for diagnosing, treating and preventing a disease using the monoclonal antibody.

The present invention discloses a B cell epitope on the surface of a glyceraldehyde-3-phosphate dehydrogenase protein, its nucleic acid molecule, and its application in detecting Mycoplasma hyopneumoniae infection. The B cell epitope of the present invention can be functionally expressed on the surface of an inert carrier S9 bacterium. Based on the LN7-antibody direct-mediated agglutination test detection method established by LN7 functionally expressed on the surface of the S9 bacterium, LN7 specifically recognizes only Mycoplasma hyopneumoniae infection serum antibodies and vaccine immune serum antibodies containing the GAPDH antigen, generating a specific binding reaction, while having no cross-reaction or non-specific binding reaction with other mycoplasma pathogens, porcine respiratory disease pathogen infection serum, and vaccine immune serum. This diagnostic method is expected to become an important technical means for the accurate diagnosis of Mycoplasma hyopneumoniae infection and the monitoring and evaluation of the immune effect of Mycoplasma hyopneumoniae vaccine.

This invention provides a non-naturally occurring genetically modified microbial organism that has been engineered to produce glyceraldehyde, glycerol, 3-hydroxypropionaldehyde or 1,3-propanediol using a pathway which enables the condensation of formyl-CoA with glycolaldehyde. This invention also provides a method for the production of glyceraldehyde, glycerol, 3-hydroxypropionaldehyde or 1,3-propanediol using a pathway which enables the condensation of formyl-CoA with glycolaldehyde. This invention additionally provides a method for the production of glycolaldehyde via the condensation of formyl-CoA and formaldehyde. Further, this invention provides a method for the formation of formyl-CoA from methane, methanol, formaldehyde or formate.

The invention relates to application of a bimetal organic framework material in production of high-added-value organic acid through hydrothermal catalysis of synergistic conversion of biomass and carbon dioxide. Transition metal Cu is introduced through an element substitution method to synthesize the bimetal organic framework material MIL-53 (Fe, Cu), and the bimetal organic framework material MIL-53 (Fe, Cu) is applied to hydrothermal catalytic biomass and CO2 synergistic conversion to produce high-added-value organic acid. On one hand, the bimetal organic framework material MIL-53 (Fe, Cu) accelerates hydrolysis of biomass derived carbohydrate-cellulose into glucose, and then promotes isomerization of glucose into fructose, reverse aldol condensation of fructose to glyceraldehyde and glyceraldehyde bond breaking to produce formaldehyde and glyceraldehyde in the cascade reaction process; further carrying out oxidation reduction with a CO2 solid source, namely NaHCO3 to produce high-added-value organic acid; on the other hand, NaHCO3 is reduced into formic acid through hydrogen production by splitting water from glucose; and finally, synergistic catalytic conversion of biomass and CO2 to high-added-value organic acid is realized.

The present invention relates to a binder composition comprising a) component A comprising polymer(s) A1 having primary and / or secondary amino groups, wherein polymer(s) A1 has(have) a primary and secondary amine group nitrogen content of at least 1 wt.-% and b) component B comprising component B1 which is 1,3-dihydroxyacetone, glycolaldehyde, glyceraldehyde or any mixture thereof, wherein the polymer(s) A1 has(have) a total weight average molecular weight Mw,total of at least 800 g / mol.

The application discloses a method for fermenting 3-hydroxypropionic acid and acrylic acid. The application provides a recombinant Corynebacterium glutamicum, wherein the ald gene of the aldehyde dehydrogenase of the microorganism is up-regulated, the expression of the glycerolaldehyde-3-phosphate dehydrogenase gapA is down-regulated, the heterologous diol dehydratase gene pduCDEGH is expressed, the heterologous 3-phospho dehydrogenase gdp and glycerol 3-phosphatase gpp are expressed. The recombinant microorganism is fermented in a shake flask or a fermenter with glucose or other organic carbon sources as the substrate to obtain 3-hydroxypropionic acid. The 3-hydroxypropionic acid in the fermentation broth is acidified and heated to further obtain acrylic acid. The recombinant microorganism can efficiently produce 3-hydroxypropionic acid and acrylic acid by using cheap glucose, sucrose, molasses and the like as raw materials, the production process is green, safe and simple, and has a good market application prospect.

The present application relates to the field of bioengineering technology, and more particularly to a kind of tiger frog environment detection sample storage reagent and its preparation method, consisting of the following components by mass fraction: preservative 95-99 parts and microcapsule 1-5 parts;The present application is composed of the composite system of environmental protection type preservative matrix and temperature-sensitive microcapsule encapsulating glycerol aldehyde-3-phosphate dehydrogenase inhibitor by design, so that the reagent has the double-stage temperature change response function of "in situ slow release under normal temperature transportation to stabilize sample microbial metabolic state" and "incubation triggered rapid release in laboratory", can maximize the preservation of tiger frog environmental sample collection instant microbial activity and community structure information, thereby significantly improving the authenticity and reliability of subsequent molecular detection results, providing an efficient and convenient tool for tiger frog habitat health assessment, biodiversity monitoring and ecological environment research, and has broad application prospect.

The present invention relates to a process for producing a foam, which comprises foaming a mixture, comprising one or more amino acid copolymers (A), one or more components (B) capable of reacting with said amino acid copolymers (A) and one or more blowing agents (F), wherein component (B) is selected from alpha-hydroxy ketones and alpha-aldehydes such as reducing sugars, 1,3-dihydroxyacetone, glycolaldehyde, glyceraldehyde or any mixture thereof and the foam obtainable by this process.

The invention discloses a biomarker for predicting prognosis of BPA-induced intrahepatic cholangiocarcinoma, a scoring model and application, and belongs to the technical field of biomedicine. The biomarker is composed of eight genes, namely, GAPDH (Glyceraldehyde-3-phosphate Dehydrogenase), HSP90AA1 (Hepatocyte Specific Protein 90AA1), CTNNB1 (Cytotoxic Nuclear Nuclear Nuclear Nuclear B1), NFKBIA (Nuclear Factor Kinase Kinase Institute), HSPA5 (Hepatocyte A risk scoring model is constructed by utilizing the biomarkers, and by calculating risk scores, clinical recognition of intrahepatic cholangiocarcinoma prognosis high-risk patients can be assisted in advance, so that an effective strategy can be adopted in an early stage of a disease, the recurrence probability is reduced, the lifetime of the patients is prolonged, and an accurate basis is provided for formulating an individualized treatment scheme.

The application belongs to the technical field of biological medicine, and discloses application of oridonin in preparation of a medicine for treating rheumatoid arthritis synovitis. For the first time, it is confirmed that oridonin takes glyceraldehyde-3-phosphate dehydrogenase as a direct action target, covalently combines through an α, β-unsaturated ketone, inhibits enzyme activity, blocks abnormal glycolysis of rheumatoid arthritis synovial cells, activates a KEAP1-NRF2 / HO-1 antioxidant anti-inflammatory pathway, inhibits an NF-κB inflammatory signal, and intervenes in a pathological process of rheumatoid arthritis synovitis from multiple dimensions, thereby providing a scientific basis for clinical application and deep development of oridonin against RA synovitis. A medicine composition containing oridonin and application of the medicine composition in preparation of an anti-rheumatoid arthritis synovitis medicine are disclosed. The application has advantages of target point precision, remarkable curative effect, high safety, mechanism innovation and the like, and provides a brand-new scheme for clinical treatment of rheumatoid arthritis synovitis.

The present disclosure concerns recombinant yeast host cells having a first genetic modification for downregulating a first metabolic pathway that converts NADP+ to NADPH, as well as a second genetic modification for upregulating a second metabolic pathway that converts NADP+ to NADPH. The second genetic modification allows the expression of a glyceraldehyde-3-phosphate dehydrogenase lacking phosphorylating activity, which can, in some embodiments, be from enzyme commission 1.2.1.9 or 1.2.1.90. The second pathway is distinct from the first metabolic pathway. The present disclosure also concerns a process for making and improving the yield of a fermented product, such as ethanol, using the recombinant yeast host cell.