The invention discloses a
lactic dehydrogenase detection kit and a preparation method of the kit. The kit disclosed by the invention consists of a
reagent I and a
reagent II which are independent each other, wherein the components of the
reagent I comprise a biobuffer, a
metal ion complex, a
lactic dehydrogenase reactive substrate, a surfactant, a
lactic dehydrogenase activity activating agent, a
preservative and water; and the components of the reagent II comprise a biobuffer, a
nicotinamide adenine dinucleotide
oxidation state, a surfactant, a
preservative and water. The detection kit disclosed by the invention adopts a dual reagent mode to effectively avoid the interference of the nonspecific reaction and synchronously furthest reduce the interference of the sample
turbidity, thereby guaranteeing the stable and reliable measurement result; and the detection kit has the advantages of good stability, high precision, wide linear testing range, good
repeatability, and strong anti-interference performance and the like. In addition, the detection kit disclosed by the invention does not need to pre-dilute the sample in the detecting process, thereby being convenient for clinical use, simple and fast to operate, and suitable for an automatic biochemical analyzer.