Aspartate transaminase mitochondrial isozyme detection kit

A mitochondrial isoenzyme, aspartate amino technology, applied in the field of medical testing, can solve problems such as the reduction of antibody biological activity, achieve the effect of improving stability, meeting the needs of clinical detection and diagnosis, and ensuring stability

Active Publication Date: 2015-03-25
NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, according to common knowledge in the field, when antibodies are stored in a solution state for a long time, chemical decomposition products, insoluble aggregates, and soluble associates will occur, which will greatly reduce the biological...

Method used

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  • Aspartate transaminase mitochondrial isozyme detection kit
  • Aspartate transaminase mitochondrial isozyme detection kit
  • Aspartate transaminase mitochondrial isozyme detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A mAST detection kit, comprising reagent 1 and reagent 2, wherein:

[0023] Reagent 1:

[0024]

[0025] Reagent 2:

[0026]

Embodiment 2

[0028] A mAST detection kit, comprising reagent 1 and reagent 2, wherein:

[0029] Reagent 1:

[0030]

[0031]

[0032] Reagent 2:

[0033]

[0034] The stability performance of the reagent obtained in Example 1 of the present invention will be described below.

[0035] 1. Antibody stability:

[0036] Configure the following three groups of reagents as follows:

[0037] Group ①: Prepare the reagent according to the formula described in Example 1, and store it at 2-8°C for 18 months;

[0038] Group ②: Except that no antibody stabilizer was added, the reagents were prepared according to the formula described in Example 1, and stored at 2-8°C for 18 months;

[0039] Group ③: After 18 months, the reagents were newly prepared according to the formula described in Example 1 (the antibodies added were the same batch of antibodies frozen at -20°C).

[0040] Put the above three groups of reagents on the automatic biochemical analyzer, set the measurement parameters accor...

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Abstract

The invention discloses an aspartate transaminase mitochondrial isozyme detection kit, comprising: reagents 1: 0.02-0.5mol/L of buffer solution of which pH value is 6.5-8.0, 0.15-0.3mol/L of L-potassium aspartate, 0.3-5KU/L of malic dehydrogenase, 0.3-5KU/L of lactic dehydrogenase, 5-20mL/L of human cAST antibody, 0.01-3% of antibody stabilizer, 0.01-10% of enzyme stabilizer and 0.01-1% of preservative; and reagents 2: 0.02-0.5mol/L of buffer solution of which pH value is 7.0-9.0, 0.005-0.02mol/L of alpha-ketoglutarate, 0.1-0.5mmol/L of reduced coenzyme I, 0.01-1% of preservative and 0.01-10% of reduced coenzyme I stabilizer. The kit has the advantage of good stability.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to a detection kit for aspartate aminotransferase mitochondrial isozyme (m-AST or mAST mitochondrial aspartate aminotransferase). Background technique [0002] Aspartate aminotransferase (Aspartate aminotransferase, AST) widely exists in various tissue cells of the human body, especially in liver and heart tissue cells, while the activity of AST in normal human serum is very low. There are two isoenzymes of AST in the human body, one is cAST, which is mainly derived from the cytoplasm, and the other is mAST, which exists in the mitochondria. The two isozymes differ in genetic composition, amino acid composition, immunological properties, and half-life in vivo. [0003] When the body is mildly diseased, the cell membrane permeability of tissue cells increases, cAST enters the blood system through the cell membrane, but mAST is protected by two layers of mitochondrial membran...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/68
Inventor 邹炳德邹继华陆慧贤黄辛雷
Owner NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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