Aspartate transaminase mitochondrial isozyme detection kit

Abstract

The invention discloses an aspartate transaminase mitochondrial isozyme detection kit, comprising: reagents 1: 0.02-0.5mol / L of buffer solution of which pH value is 6.5-8.0, 0.15-0.3mol / L of L-potassium aspartate, 0.3-5KU / L of malic dehydrogenase, 0.3-5KU / L of lactic dehydrogenase, 5-20mL / L of human cAST antibody, 0.01-3% of antibody stabilizer, 0.01-10% of enzyme stabilizer and 0.01-1% of preservative; and reagents 2: 0.02-0.5mol / L of buffer solution of which pH value is 7.0-9.0, 0.005-0.02mol / L of alpha-ketoglutarate, 0.1-0.5mmol / L of reduced coenzyme I, 0.01-1% of preservative and 0.01-10% of reduced coenzyme I stabilizer. The kit has the advantage of good stability.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to a detection kit for aspartate aminotransferase mitochondrial isozyme (m-AST or mAST mitochondrial aspartate aminotransferase). Background technique [0002] Aspartate aminotransferase (Aspartate aminotransferase, AST) widely exists in various tissue cells of the human body, especially in liver and heart tissue cells, while the activity of AST in normal human serum is very low. There are two isoenzymes of AST in the human body, one is cAST, which is mainly derived from the cytoplasm, and the other is mAST, which exists in the mitochondria. The two isozymes differ in genetic composition, amino acid composition, immunological properties, and half-life in vivo. [0003] When the body is mildly diseased, the cell membrane permeability of tissue cells increases, cAST enters the blood system through the cell membrane, but mAST is protected by two layers of mitochondrial membran...

AI Technical Summary

Problems solved by technology

However, according to common knowledge in the field, when antibodies are stored in a solution state for a long time, chemical decomposition products, insoluble aggregates, and soluble associates will occur, which will greatly reduce the biological activity of the antibody. Therefore, most antibodies It is best to store it at -20°C. In order to maintain the activity of the antibody, the kit can only be temporarily configured during use, which is obviously not suitable for commercial liquid kits.

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